Rorug01G0299400

Lysine-specific histone demethylase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005668
Physical Location & Seq
Forward (+)
41590154 .. 41592025
1872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0299400.1

Sequence Viewer

Length: 834 bp
ATGAAGAGAATAGGAGGAGTAGCCGAGAAAATGCACGTGCTGGAGATCAACCTGATCTCCGGTCAGGGCTTGAAAGTCCCGTCCGGGAAGTTACGGCGGATGAAGACCTACGCAATCGCCTGGGTCGACTCGGACCACAAGATCCGGACCCGGGTCGACAAAGTCGGAGCCGAGAACCCGACTTGGAACGACCGGTTCCTCTTCAAGGTCTCCTCCGAATTCCTCGCCAGGGAGACGTCCGGGATCACGATCGAGATCTACGCCGTCGGTGTCATCCGCGACCATCTCGTCGGCACCGTCCGATTGATCCTCAGCAACTTTCTTGACCTGGAATCCAAGATCCCGTCGTTCACGGCGCTGCAGATTCGCCGGCCTTCTGGAAGATTCCACGGCGTGTTGAACGTCGCCGCGTTGGTCAATCACGAGTCGGAGTGGTCCAAGGAGCTCGACGACGTCTCGGCGATCGGGTACCAAGATCTGATGGGCAAGGGAGAGAGCTTCCGGCGCCGGCGCCGGAGGGACAGCTTGACGAGGTCTACCGAGAATTTCTCCGGCAGCGAGTCGAGGGAGAATTCTTGCGCTGAGTCGGTGGAGAACTCCGACTGCGGCGACTCGTTCAAATCGGCGTCTCCGCCGTATAAATCGCCGTCTAAACCGACGTCTCCGGCGGCGGAGAAGAGAGCGCTGAAGGAGGCGAACGGCGTGAGGGATCTAGCGGCCGGAACCAGGGCTCTGAAGACAGCTGTCGCATCGGACGGCTCGCGATTCTTGTGCTGCTTGCTCACGACGCAGAGGAAGGTTCAGTCATCGTCGGATAAGAACGCAGAAAGGTGA

Protein Analysis

277

Amino Acids

30.66

Weight (kDa)

9.61

Isoelectric Point (pI)

53.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 13 - 111 8.3e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 287
AatII GACGTC 3 cut(s) 239, 456, 662
Acc65I GGTACC 1 cut(s) 468
AccB1I GGYRCC 4 cut(s) 293, 468, 504, 510
AccI GTMKAC 3 cut(s) 126, 156, 536
AccII CGCG 3 cut(s) 279, 410, 763
AccIII TCCGGA 1 cut(s) 144
AciI CCGC 8 cut(s) 97, 277, 408, 606, 632, 668, 671, 716
AclWI GGATC 5 cut(s) 136, 251, 301, 334, 717
AcoI YGGCCR 1 cut(s) 717
AcsI RAATTY 3 cut(s) 218, 544, 571
AcuI CTGAAG 2 cut(s) 707, 755
AcvI CACGTG 1 cut(s) 37
AcyI GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 659
AdeI CACNNNGTG 1 cut(s) 394
AfaI GTAC 1 cut(s) 470
AfeI AGCGCT 1 cut(s) 684
AfiI CCNNNNNNNGG 4 cut(s) 151, 205, 229, 726
AgeI ACCGGT 1 cut(s) 192
AgsI TTSAA 4 cut(s) 73, 205, 400, 619
AjnI CCWGG 4 cut(s) 119, 227, 327, 725
AluBI AGCT 4 cut(s) 445, 498, 525, 743
AluI AGCT 4 cut(s) 445, 498, 525, 743
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 5 cut(s) 214, 227, 460, 633, 666
AlwI GGATC 5 cut(s) 136, 251, 301, 334, 717
Ama87I CYCGRG 1 cut(s) 150
Aor13HI TCCGGA 1 cut(s) 144
Aor51HI AGCGCT 1 cut(s) 684
AoxI GGCC 2 cut(s) 371, 717
ApeKI GCWGC 3 cut(s) 358, 555, 774
ApoI RAATTY 3 cut(s) 218, 544, 571
AsiGI ACCGGT 1 cut(s) 192
Asp718I GGTACC 1 cut(s) 468
AspLEI GCGC 5 cut(s) 358, 507, 513, 581, 685
AspS9I GGNCC 3 cut(s) 133, 147, 435
AsuC2I CCSGG 4 cut(s) 85, 151, 152, 241
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 3 cut(s) 133, 147, 435
BanI GGYRCC 4 cut(s) 293, 468, 504, 510
BanII GRGCYC 2 cut(s) 447, 733
BauI CACGAG 1 cut(s) 422
BbrPI CACGTG 1 cut(s) 37
BbsI GAAGAC 2 cut(s) 110, 743
Bbv12I GWGCWC 1 cut(s) 447
BbvCI CCTCAGC 1 cut(s) 311
BbvI GCAGC 3 cut(s) 345, 567, 761
BccI CCATC 2 cut(s) 291, 475
BceAI ACGGC 8 cut(s) 110, 248, 369, 406, 619, 631, 715, 772
BcgI CGANNNNNNTGC 2 cut(s) 753, 787
BciT130I CCWGG 4 cut(s) 121, 229, 329, 727
BcnI CCSGG 4 cut(s) 85, 151, 152, 241
BcoDI GTCTC 5 cut(s) 214, 227, 460, 633, 666
BfaI CTAG 1 cut(s) 713
BfmI CTRYAG 1 cut(s) 359
BfoI RGCGCY 4 cut(s) 359, 508, 514, 686
BglII AGATCT 2 cut(s) 255, 475
BisI GCNGC 7 cut(s) 359, 408, 556, 607, 669, 717, 775
BlsI GCNGC 7 cut(s) 360, 409, 557, 608, 670, 718, 776
Bme1390I CCNGG 8 cut(s) 85, 121, 151, 152, 229, 241, 329, 727
Bme18I GGWCC 3 cut(s) 133, 147, 435
BmeT110I CYCGRG 1 cut(s) 150
BmgT120I GGNCC 3 cut(s) 133, 147, 435
BmiI GGNNCC 8 cut(s) 149, 169, 197, 295, 470, 506, 512, 724
BmrFI CCNGG 8 cut(s) 85, 121, 151, 152, 229, 241, 329, 727
BmsI GCATC 1 cut(s) 758
BoxI GACNNNNGTC 2 cut(s) 152, 743
BpiI GAAGAC 2 cut(s) 110, 743
BplI GAGNNNNNCTC 2 cut(s) 533, 565
BpmI CTGGAG 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 311
BpuMI CCSGG 4 cut(s) 85, 151, 152, 241
BsaAI YACGTR 1 cut(s) 37
BsaBI GATNNNNATC 2 cut(s) 248, 254
BsaHI GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 659
BsaI GGTCTC 1 cut(s) 214
BsaJI CCNNGG 6 cut(s) 120, 150, 228, 388, 438, 726
BsaWI WCCGGW 3 cut(s) 59, 144, 192
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 4 cut(s) 151, 205, 229, 726
Bse118I RCCGGY 3 cut(s) 192, 369, 507
Bse8I GATNNNNATC 2 cut(s) 248, 254
BseAI TCCGGA 1 cut(s) 144
BseBI CCWGG 4 cut(s) 121, 229, 329, 727
BseDI CCNNGG 6 cut(s) 120, 150, 228, 388, 438, 726
BseGI GGATG 2 cut(s) 105, 273
BseJI GATNNNNATC 2 cut(s) 248, 254
BseLI CCNNNNNNNGG 4 cut(s) 151, 205, 229, 726
BseMII CTCAG 2 cut(s) 325, 573
BseRI GAGGAG 2 cut(s) 30, 202
BseX3I CGGCCG 1 cut(s) 717
BseXI GCAGC 3 cut(s) 345, 567, 761
Bsh1236I CGCG 3 cut(s) 279, 410, 763
Bsh1285I CGRYCG 4 cut(s) 193, 252, 465, 720
BshFI GGCC 2 cut(s) 373, 719
BshNI GGYRCC 4 cut(s) 293, 468, 504, 510
BshTI ACCGGT 1 cut(s) 192
BsiEI CGRYCG 4 cut(s) 193, 252, 465, 720
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 150
BslFI GGGAC 2 cut(s) 62, 533
BslI CCNNNNNNNGG 4 cut(s) 151, 205, 229, 726
BsmAI GTCTC 5 cut(s) 214, 227, 460, 633, 666
BsmBI CGTCTC 4 cut(s) 227, 460, 633, 666
BsmFI GGGAC 2 cut(s) 62, 533
BsnI GGCC 2 cut(s) 373, 719
Bso31I GGTCTC 1 cut(s) 214
BsoBI CYCGRG 1 cut(s) 150
Bsp1286I GDGCHC 2 cut(s) 447, 733
Bsp13I TCCGGA 1 cut(s) 144
Bsp68I TCGCGA 1 cut(s) 763
BspACI CCGC 8 cut(s) 97, 277, 408, 606, 632, 668, 671, 716
BspANI GGCC 2 cut(s) 373, 719
BspCNI CTCAG 2 cut(s) 324, 574
BspEI TCCGGA 1 cut(s) 144
BspFNI CGCG 3 cut(s) 279, 410, 763
BspLI GGNNCC 8 cut(s) 149, 169, 197, 295, 470, 506, 512, 724
BspMAI CTGCAG 1 cut(s) 363
BspPI GGATC 5 cut(s) 136, 251, 301, 334, 717
BspT107I GGYRCC 4 cut(s) 293, 468, 504, 510
BspTNI GGTCTC 1 cut(s) 214
BsrFI RCCGGY 3 cut(s) 192, 369, 507
BssAI RCCGGY 3 cut(s) 192, 369, 507
BssECI CCNNGG 6 cut(s) 120, 150, 228, 388, 438, 726
BssNI GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 659
BssSI CACGAG 1 cut(s) 422
BssT1I CCWWGG 1 cut(s) 438
Bst2BI CACGAG 1 cut(s) 422
Bst2UI CCWGG 4 cut(s) 121, 229, 329, 727
Bst4CI ACNGT 1 cut(s) 298
Bst6I CTCTTC 2 cut(s) 206, 671
BstACI GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 659
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 4 cut(s) 371, 509, 761, 779
BstDEI CTNAG 2 cut(s) 311, 582
BstDSI CCRYGG 1 cut(s) 388
BstENI CCTNNNNNAGG 1 cut(s) 203
BstF5I GGATG 2 cut(s) 105, 273
BstFNI CGCG 3 cut(s) 279, 410, 763
BstH2I RGCGCY 4 cut(s) 359, 508, 514, 686
BstHHI GCGC 5 cut(s) 358, 507, 513, 581, 685
BstMAI GTCTC 5 cut(s) 214, 227, 460, 633, 666
BstMCI CGRYCG 4 cut(s) 193, 252, 465, 720
BstMWI GCNNNNNNNGC 2 cut(s) 504, 787
BstNI CCWGG 4 cut(s) 121, 229, 329, 727
BstPAI GACNNNNGTC 2 cut(s) 152, 743
BstSCI CCNGG 8 cut(s) 83, 119, 149, 150, 227, 239, 327, 725
BstSFI CTRYAG 1 cut(s) 359
BstUI CGCG 3 cut(s) 279, 410, 763
BstV1I GCAGC 3 cut(s) 345, 567, 761
BstV2I GAAGAC 2 cut(s) 110, 743
BstX2I RGATCY 5 cut(s) 141, 255, 339, 475, 709
BstYI RGATCY 5 cut(s) 141, 255, 339, 475, 709
BstZI CGGCCG 1 cut(s) 717
BsuRI GGCC 2 cut(s) 373, 719
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 2 cut(s) 105, 273
BtuMI TCGCGA 1 cut(s) 763
Cac8I GCNNGC 4 cut(s) 371, 509, 761, 779
CfoI GCGC 5 cut(s) 358, 507, 513, 581, 685
Cfr10I RCCGGY 3 cut(s) 192, 369, 507
Cfr13I GGNCC 3 cut(s) 133, 147, 435
Cfr9I CCCGGG 1 cut(s) 150
CseI GACGC 2 cut(s) 615, 796
Csp6I GTAC 1 cut(s) 469
CspAI ACCGGT 1 cut(s) 192
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 2 cut(s) 311, 582
DinI GGCGCC 2 cut(s) 506, 512
DraIII CACNNNGTG 1 cut(s) 394
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 1 cut(s) 717
EagI CGGCCG 1 cut(s) 717
Eam1104I CTCTTC 2 cut(s) 206, 671
EarI CTCTTC 2 cut(s) 206, 671
EciI GGCGGA 3 cut(s) 112, 621, 686
Ecl136II GAGCTC 1 cut(s) 445
EclXI CGGCCG 1 cut(s) 717
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 2 cut(s) 447, 733
Eco31I GGTCTC 1 cut(s) 214
Eco47I GGWCC 3 cut(s) 133, 147, 435
Eco47III AGCGCT 1 cut(s) 684
Eco52I CGGCCG 1 cut(s) 717
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 707, 755
Eco72I CACGTG 1 cut(s) 37
Eco88I CYCGRG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 445
EcoNI CCTNNNNNAGG 1 cut(s) 203
EcoRI GAATTC 2 cut(s) 218, 571
EcoRII CCWGG 4 cut(s) 119, 227, 327, 725
EcoT14I CCWWGG 1 cut(s) 438
EcoT38I GRGCYC 2 cut(s) 447, 733
EgeI GGCGCC 2 cut(s) 506, 512
EheI GGCGCC 2 cut(s) 506, 512
ErhI CCWWGG 1 cut(s) 438
Esp3I CGTCTC 4 cut(s) 227, 460, 633, 666
FaiI YATR 1 cut(s) 639
FaqI GGGAC 2 cut(s) 62, 533
FblI GTMKAC 3 cut(s) 126, 156, 536
Fnu4HI GCNGC 7 cut(s) 359, 408, 556, 607, 669, 717, 775
FokI GGATG 2 cut(s) 112, 260
FriOI GRGCYC 2 cut(s) 447, 733
Fsp4HI GCNGC 7 cut(s) 359, 408, 556, 607, 669, 717, 775
FspBI CTAG 1 cut(s) 713
GlaI GCGC 5 cut(s) 357, 506, 512, 580, 684
GluI GCNGC 7 cut(s) 359, 408, 556, 607, 669, 717, 775
GsuI CTGGAG 1 cut(s) 62
HaeII RGCGCY 4 cut(s) 359, 508, 514, 686
HaeIII GGCC 2 cut(s) 373, 719
HgaI GACGC 2 cut(s) 615, 796
HhaI GCGC 5 cut(s) 358, 507, 513, 581, 685
Hin1I GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 659
Hin6I GCGC 5 cut(s) 356, 505, 511, 579, 683
HinP1I GCGC 5 cut(s) 356, 505, 511, 579, 683
HincII GTYRAC 2 cut(s) 127, 157
HindII GTYRAC 2 cut(s) 127, 157
HinfI GANTC 9 cut(s) 128, 332, 364, 384, 425, 560, 584, 611, 765
Hpy166II GTNNAC 4 cut(s) 127, 157, 351, 537
Hpy188III TCNNGA 8 cut(s) 145, 247, 253, 323, 378, 422, 762, 784
Hpy8I GTNNAC 4 cut(s) 127, 157, 351, 537
Hpy99I CGWCG 9 cut(s) 269, 293, 349, 407, 452, 455, 661, 790, 814
HpyAV CCTTC 3 cut(s) 384, 682, 790
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 5 cut(s) 36, 236, 402, 453, 659
HpyCH4V TGCA 2 cut(s) 34, 361
HpyF10VI GCNNNNNNNGC 2 cut(s) 504, 787
HpyF3I CTNAG 2 cut(s) 311, 582
HpySE526I ACGT 5 cut(s) 36, 236, 402, 453, 659
Hsp92I GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 659
HspAI GCGC 5 cut(s) 356, 505, 511, 579, 683
KasI GGCGCC 2 cut(s) 504, 510
Kpn2I TCCGGA 1 cut(s) 144
KpnI GGTACC 1 cut(s) 472
KroI GCCGGC 2 cut(s) 369, 507
KroNI GCCGGC 2 cut(s) 371, 509
LmnI GCTCC 2 cut(s) 167, 442
Lsp1109I GCAGC 3 cut(s) 345, 567, 761
LweI GCATC 1 cut(s) 758
MaeI CTAG 1 cut(s) 713
MaeII ACGT 5 cut(s) 36, 236, 402, 453, 659
MaeIII GTNAC 1 cut(s) 90
MboII GAAGA 6 cut(s) 16, 115, 193, 393, 688, 748
MflI RGATCY 5 cut(s) 141, 255, 339, 475, 709
MhlI GDGCHC 2 cut(s) 447, 733
MluCI AATT 3 cut(s) 218, 544, 571
Mly113I GGCGCC 2 cut(s) 505, 511
MlyI GAGTC 5 cut(s) 122, 434, 569, 593, 605
MmeI TCCRAC 4 cut(s) 145, 408, 624, 792
MreI CGCCGGCG 1 cut(s) 507
MroI TCCGGA 1 cut(s) 144
MroNI GCCGGC 2 cut(s) 369, 507
MspA1I CMGCKG 1 cut(s) 743
MspR9I CCNGG 8 cut(s) 85, 121, 151, 152, 229, 241, 329, 727
MvaI CCWGG 4 cut(s) 121, 229, 329, 727
MvnI CGCG 3 cut(s) 279, 410, 763
MwoI GCNNNNNNNGC 2 cut(s) 504, 787
NaeI GCCGGC 2 cut(s) 371, 509
NarI GGCGCC 2 cut(s) 505, 511
NciI CCSGG 4 cut(s) 85, 151, 152, 241
NgoMIV GCCGGC 2 cut(s) 369, 507
NlaIV GGNNCC 8 cut(s) 149, 169, 197, 295, 470, 506, 512, 724
NmeAIII GCCGAG 3 cut(s) 49, 196, 437
NruI TCGCGA 1 cut(s) 763
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 294
PdiI GCCGGC 2 cut(s) 371, 509
PfeI GAWTC 4 cut(s) 332, 364, 384, 765
PflFI GACNNNGTC 3 cut(s) 161, 452, 532
PfoI TCCNGGA 2 cut(s) 83, 239
PinAI ACCGGT 1 cut(s) 192
PkrI GCNGC 7 cut(s) 360, 409, 557, 608, 670, 718, 776
Ple19I CGATCG 2 cut(s) 252, 465
PleI GAGTC 5 cut(s) 122, 433, 568, 592, 605
PluTI GGCGCC 2 cut(s) 508, 514
PmaCI CACGTG 1 cut(s) 37
PmlI CACGTG 1 cut(s) 37
PpsI GAGTC 5 cut(s) 122, 433, 568, 592, 605
Ppu21I YACGTR 1 cut(s) 37
PshAI GACNNNNGTC 2 cut(s) 152, 743
Psp124BI GAGCTC 1 cut(s) 447
Psp6I CCWGG 4 cut(s) 119, 227, 327, 725
PspCI CACGTG 1 cut(s) 37
PspGI CCWGG 4 cut(s) 119, 227, 327, 725
PspN4I GGNNCC 8 cut(s) 149, 169, 197, 295, 470, 506, 512, 724
PspPI GGNCC 3 cut(s) 133, 147, 435
PstI CTGCAG 1 cut(s) 363
PsuI RGATCY 5 cut(s) 141, 255, 339, 475, 709
PsyI GACNNNGTC 3 cut(s) 161, 452, 532
PvuI CGATCG 2 cut(s) 252, 465
PvuII CAGCTG 1 cut(s) 743
RruI TCGCGA 1 cut(s) 763
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
SacI GAGCTC 1 cut(s) 447
SalI GTCGAC 2 cut(s) 125, 155
SatI GCNGC 7 cut(s) 359, 408, 556, 607, 669, 717, 775
Sau96I GGNCC 3 cut(s) 133, 147, 435
SchI GAGTC 5 cut(s) 122, 434, 569, 593, 605
ScrFI CCNGG 8 cut(s) 85, 121, 151, 152, 229, 241, 329, 727
SduI GDGCHC 2 cut(s) 447, 733
SfaNI GCATC 1 cut(s) 758
SfcI CTRYAG 1 cut(s) 359
SfoI GGCGCC 2 cut(s) 506, 512
SgrAI CRCCGGYG 1 cut(s) 507
SinI GGWCC 3 cut(s) 133, 147, 435
SmaI CCCGGG 1 cut(s) 152
Sse9I AATT 3 cut(s) 218, 544, 571
SsiI CCGC 8 cut(s) 97, 277, 408, 606, 632, 668, 671, 716
SspDI GGCGCC 2 cut(s) 504, 510
SspMI CTAG 1 cut(s) 713
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 8 cut(s) 83, 119, 149, 150, 227, 239, 327, 725
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 5 cut(s) 39, 239, 405, 456, 662
TaqI TCGA 5 cut(s) 126, 156, 252, 447, 563
TasI AATT 3 cut(s) 218, 544, 571
TauI GCSGC 4 cut(s) 410, 609, 671, 719
TfiI GAWTC 4 cut(s) 332, 364, 384, 765
TseI GCWGC 3 cut(s) 358, 555, 774
TspDTI ATGAA 2 cut(s) 17, 116
TspMI CCCGGG 1 cut(s) 150
Tth111I GACNNNGTC 3 cut(s) 161, 452, 532
VpaK11BI GGWCC 3 cut(s) 133, 147, 435
XagI CCTNNNNNAGG 1 cut(s) 203
XapI RAATTY 3 cut(s) 218, 544, 571
XmaI CCCGGG 1 cut(s) 150
XmiI GTMKAC 3 cut(s) 126, 156, 536
XspI CTAG 1 cut(s) 713
ZraI GACGTC 3 cut(s) 237, 454, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.