FvH4_7g18750

Lysine-specific histone demethylase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005668
Physical Location & Seq
Reverse (-)
15568125 .. 15573276
5152 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18750.t4

Sequence Viewer

Length: 2736 bp
ATGAATCCTCCGAACGAATTCCCCGACGACTTCTCGTCTTTCCCCCCTATCCCATTCGCCCTCGTCGTCCCTCCTGAAAACCCTAACCCTAACCCTAACCCCAACCCTAATCCAACCCTGCCGCCAAGTCAGACCCCACTCGAAGACCCTAGCTCGTCTCATCCACTCTCATTCACGGTCCCGAAGAAGCGGAGGCGCGGCCGGCCTCATCGTCCCGCGACGTCGTTTCACCTCCCCCAATTCCTCAACGGCGGCGTCAATAGTAACGGTAACAGTCTCGCTTCTTCTTCTGGTATCTCAATTCCAGCTAATTCGATTAGACACAATGTGGAAAACCCTAGCTCGAAACCGGCGCCGGATATGTCCGACGAGATCATCGTGATCAATAAGGAGTCCACGGCCGAGGCCCTGATCGCGCTCTCGGCCGGGTTTCCGGCCGATTCCCTCACCGAGGAGGAAATCGATTTCGGAATTATTAGAGTGATTGGGGGAATAGAGCAGGTGAACTACATTCTTATTCGGAACCATATTATTGCGAAATGGCGCGAAAATGTGTCCAATTGGGTTGCCAAGGACATTTTTGTGAACTCTATACCCAAGCATTGCCATGGTCTTTTGGATTCTACTTATAACTATCTTGTTTCGCATGGATATATTAACTTTGGGATTGCGCCAGCTATTAAAGAGAAGATTCCTGTTGAGGCTAGTAAGCCGAGTGTGATCATCATTGGTGCAGGGCTGGCTGGTCTTGCTGCTGCGAGGCAGATGATGCGGTTCGGGTTTAAGGTGACGGTTTTGGAGGGTAGGAAGCGTGCGGGCGGGCGTGTGTATACTAAGAAAATGGAGGGAGGGATTAGGGTGACACAAGCAGCAGCGGATTTAGGTGGTAGTGTTTTGACTGGTACGTTAGGGAACCCGCTTGGCATTGTTGCGCGGCAGTTGGGGTACTCGCTTCATAAGATTAGAGACAAGTGCCCGCTTTATAATGTAGAGGGGCAGCCTGTGGATCATGATATGGATATGAAGGTGGAAACTGTTTTTAACCAACTTTTAGATAAGGCAAGTAGGCTTAGGCAGTCAATGGGTGGGGTTTCGGTTGATGTGTCTCTTGGTTCGGCATTGGAAACGTTCTGGCAGGTTTCTGCGAATGCTGTGAATGCTGAGGAGATGAACTTATTCAACTGGCATCTTGCAAATTTGGAGTATGCTAATGCCGGTTTACTATCAAAACTTTCGCTTGCATTTTGGGACCAAGATGATCCGTATGATATGGGAGGGGACCATTGCTTCTTACCTGGTGGTAATGGCCGGCTGGTGCAGGCTTTGGCAGAGAATGTGCCGATCCTGTATGAGAAAACTGTTCACACCATTAGGTATGGTAGTGACGGGGTACAGATTCTCGCTGGAAGCCAGGTTTTTGAGGGCGATATGGTTTTGTGCACTGTTCCGCTAGGTGTTTTGAAGAGTGGGTCCATCAAGTTTATTCCAGAGTTGCCTCAGCGAAAGCTTGATGGAATTAAGAGGCTGGGGTTTGGGCTGTTGAATAAGGTTGCAATGCTTTTTCCTCATGTGTTTTGGGGCACAGATCTTGATACCTTCGGCCACCTCTCTGATGATTCAAGCCGTCGAGGGGAATTCTTCCTGTTTTATAGCTATGCAACAGTTGCTGGTGGTCCTCTCTTGCTTGCTTTAGTAGCAGGTGAAGCTGCACATAAGTTTGAGAGCATGCCCCCTACCGATGCTGTGACCAGGGTTCTGCAGATTCTTAAGGGTATCTATGAACCACAAGGTATTAGTGTCCCAGAGCCCATCCAAACTGTGTGTACCAGATGGGGAAGTGACCCCTTCAGCCTGGGTTCTTACTCTAATGTTGCAGTTGGGGCATCAGGAGATGATTATGATATTTTAGCAGAAAGTGTTGGAGATGGGCGTCTTTTCTTTGCCGGTGAGGCTACCAATAGGAGATATCCCGCAACTATGCATGGAGCTTTCCTTAGTGGTCTCAGAGAAGCCGCCAATATGGCTCACTATGCTAATGCTCGAGCTCTGAGGATGAAGGTTAACAGGAACCCATCAAAGAATGCTCATTCTTGTGCTTCCCTTCTTGCAGATTTATTCAGAGAACCTGATTTAGAATTTGGAAGTTTTTCTGTTATTTTTGGTCAAAGAAATGCTGATCCCAAGTCTACAGCAATTTTAAGGGTGACATTTAATGACCCTCGGAAGAAGAGTCATGAAGGATCAAGACCTGATCAACAGCACTCAAATAAGTTACTTTTTCAGCAGCTTCAGTCACATTTTAATCAGCAGCAGCAGCTTCATGTTTACACTTTGTTATCTAGGCAACAGGCTCTTGAGCTCCGCGAAGTACGAGGTGGTGATGAGATGAGGTTGAATTACCTCTGTGAAAAGCTTGGGGTGAAGCTTGTGGGGAGAAAGGGTCTGGGGCCTTCAGCTGATTCTGTGATTGCCTTAATAAAAGCTGAGAGGGGCAATCGTAAACCAGCTTCAACTTTATCATCTTTGAAGGCTGGGACATCGAAGCTAAAACCTGGCACTTTCAAACGAAAAATGGTCAGAAGGGCTAAAATACTGCGTAGCATTAATGCGTCTCCCCTTTCTGGTAATTCAAACTTGGCTATTGGTAAACTCCCAGAGGAAAATAAGAACAGTGTTCAAGCAGTCTCCAGCACACTCGGTTCAGGCCAAAACAATACTGACGTGTTGAAGCCATGA

Protein Analysis

912

Amino Acids

99.16

Weight (kDa)

9.02

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIRM PF04433 147 - 221 3.5e-13 SWIRM domain
Pyr_redox_2 PF07992 239 - 292 3.7e-06 Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8 PF13450 242 - 299 2.9e-10 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 247 - 674 1.7e-84 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 630, 984
AarI CACCTGC 2 cut(s) 490, 1688
AatII GACGTC 1 cut(s) 224
Acc36I ACCTGC 3 cut(s) 490, 1126, 1688
AccB1I GGYRCC 1 cut(s) 352
AccI GTMKAC 2 cut(s) 830, 2186
AccII CGCG 6 cut(s) 198, 218, 416, 546, 934, 2364
AclI AACGTT 1 cut(s) 1127
AclWI GGATC 5 cut(s) 1014, 1253, 1336, 2171, 2248
AcoI YGGCCR 6 cut(s) 199, 399, 423, 435, 1306, 1600
AcsI RAATTY 4 cut(s) 17, 1195, 1634, 2135
AcuI CTGAAG 3 cut(s) 1831, 2273, 2436
AcyI GRCGYC 4 cut(s) 221, 255, 353, 1930
AdeI CACNNNGTG 1 cut(s) 328
AfaI GTAC 5 cut(s) 904, 947, 1392, 1825, 2370
AfiI CCNNNNNNNGG 3 cut(s) 451, 1630, 2621
AflII CTTAAG 1 cut(s) 1766
AflIII ACRYGT 1 cut(s) 2721
AhdI GACNNNNNGTC 1 cut(s) 34
AjiI CACGTC 1 cut(s) 2722
AjnI CCWGG 5 cut(s) 1294, 1410, 1748, 1851, 2551
Alw21I GWGCWC 3 cut(s) 1442, 2047, 2361
Alw26I GTCTC 7 cut(s) 162, 281, 960, 1110, 2006, 2616, 2689
Alw44I GTGCAC 1 cut(s) 1438
AlwI GGATC 5 cut(s) 1014, 1253, 1336, 2171, 2248
AlwNI CAGNNNCTG 1 cut(s) 1667
Ama87I CYCGRG 1 cut(s) 2040
ApaLI GTGCAC 1 cut(s) 1438
ApoI RAATTY 4 cut(s) 17, 1195, 1634, 2135
AseI ATTAAT 1 cut(s) 2604
Asp700I GAANNNNTTC 3 cut(s) 17, 1175, 2146
AspLEI GCGC 6 cut(s) 198, 355, 418, 546, 673, 934
AspS9I GGNCC 7 cut(s) 178, 406, 1249, 1279, 1470, 1673, 2447
AsuC2I CCSGG 1 cut(s) 427
AvaI CYCGRG 1 cut(s) 2040
AvaII GGWCC 5 cut(s) 178, 1249, 1279, 1470, 1673
BaeGI GKGCMC 3 cut(s) 977, 1442, 1583
BanI GGYRCC 1 cut(s) 352
BanII GRGCYC 3 cut(s) 1809, 2047, 2361
BarI GAAGNNNNNNTAC 2 cut(s) 2491, 2523
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 3 cut(s) 1442, 2047, 2361
BbvCI CCTCAGC 2 cut(s) 1161, 1497
BccI CCATC 6 cut(s) 1481, 1505, 1817, 1824, 1919, 2080
BceAI ACGGC 3 cut(s) 265, 414, 1608
BciT130I CCWGG 5 cut(s) 1296, 1412, 1750, 1853, 2553
BclI TGATCA 3 cut(s) 381, 720, 2251
BcnI CCSGG 1 cut(s) 427
BcoDI GTCTC 7 cut(s) 162, 281, 960, 1110, 2006, 2616, 2689
BfaI CTAG 5 cut(s) 150, 339, 705, 1451, 2340
BfmI CTRYAG 2 cut(s) 1757, 2187
BfoI RGCGCY 1 cut(s) 356
BfrI CTTAAG 1 cut(s) 1766
BfuAI ACCTGC 3 cut(s) 490, 1126, 1688
BglI GCCNNNNNGGC 2 cut(s) 1949, 2021
BglII AGATCT 1 cut(s) 1585
Bme1390I CCNGG 6 cut(s) 427, 1296, 1412, 1750, 1853, 2553
Bme18I GGWCC 5 cut(s) 178, 1249, 1279, 1470, 1673
BmeRI GACNNNNNGTC 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 2040
BmgBI CACGTC 1 cut(s) 2722
BmgT120I GGNCC 7 cut(s) 178, 406, 1249, 1279, 1470, 1673, 2447
BmiI GGNNCC 9 cut(s) 180, 354, 524, 914, 1250, 1280, 1471, 2069, 2448
BmrFI CCNGG 6 cut(s) 427, 1296, 1412, 1750, 1853, 2553
BmsI GCATC 4 cut(s) 759, 1195, 1729, 1892
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 2671
Bpu10I CCTNAGC 3 cut(s) 1070, 1161, 1497
BpuEI CTTGAG 1 cut(s) 2375
BpuMI CCSGG 1 cut(s) 427
Bsa29I ATCGAT 1 cut(s) 462
BsaHI GRCGYC 4 cut(s) 221, 255, 353, 1930
BsaI GGTCTC 1 cut(s) 2006
BsaJI CCNNGG 8 cut(s) 396, 402, 450, 570, 607, 1749, 1852, 2219
BsaXI ACNNNNNCTCC 2 cut(s) 2425, 2455
Bsc4I CCNNNNNNNGG 3 cut(s) 451, 1630, 2621
Bse118I RCCGGY 5 cut(s) 201, 349, 1214, 1308, 1943
Bse1I ACTGG 2 cut(s) 904, 1187
Bse3DI GCAATG 3 cut(s) 601, 1282, 1560
BseBI CCWGG 5 cut(s) 1296, 1412, 1750, 1853, 2553
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 8 cut(s) 396, 402, 450, 570, 607, 1749, 1852, 2219
BseGI GGATG 3 cut(s) 160, 1809, 2058
BseLI CCNNNNNNNGG 3 cut(s) 451, 1630, 2621
BseMI GCAATG 3 cut(s) 601, 1282, 1560
BseMII CTCAG 5 cut(s) 1152, 1511, 2017, 2039, 2475
BseNI ACTGG 2 cut(s) 904, 1187
BseRI GAGGAG 2 cut(s) 467, 1178
BseSI GKGCMC 3 cut(s) 977, 1442, 1583
BseX3I CGGCCG 4 cut(s) 199, 399, 423, 435
BseYI CCCAGC 2 cut(s) 1525, 2531
BsgI GTGCAG 3 cut(s) 753, 1337, 1692
Bsh1236I CGCG 6 cut(s) 198, 218, 416, 546, 934, 2364
Bsh1285I CGRYCG 4 cut(s) 202, 402, 426, 438
BshNI GGYRCC 1 cut(s) 352
BshVI ATCGAT 1 cut(s) 462
BsiEI CGRYCG 4 cut(s) 202, 402, 426, 438
BsiHKAI GWGCWC 3 cut(s) 1442, 2047, 2361
BsiHKCI CYCGRG 1 cut(s) 2040
BsiSI CCGG 8 cut(s) 202, 350, 356, 426, 434, 1215, 1309, 1944
BslFI GGGAC 7 cut(s) 53, 164, 198, 1262, 1292, 1784, 2548
BslI CCNNNNNNNGG 3 cut(s) 451, 1630, 2621
BsmAI GTCTC 7 cut(s) 162, 281, 960, 1110, 2006, 2616, 2689
BsmBI CGTCTC 2 cut(s) 162, 2616
BsmFI GGGAC 7 cut(s) 53, 164, 198, 1262, 1292, 1784, 2548
BsmI GAATGC 3 cut(s) 1153, 1162, 2086
Bso31I GGTCTC 1 cut(s) 2006
BsoBI CYCGRG 1 cut(s) 2040
Bsp1286I GDGCHC 6 cut(s) 977, 1442, 1583, 1809, 2047, 2361
Bsp19I CCATGG 1 cut(s) 607
BspCNI CTCAG 5 cut(s) 1153, 1510, 2016, 2040, 2476
BspDI ATCGAT 1 cut(s) 462
BspFNI CGCG 6 cut(s) 198, 218, 416, 546, 934, 2364
BspHI TCATGA 2 cut(s) 1009, 2233
BspLI GGNNCC 9 cut(s) 180, 354, 524, 914, 1250, 1280, 1471, 2069, 2448
BspMAI CTGCAG 1 cut(s) 1761
BspMI ACCTGC 3 cut(s) 490, 1126, 1688
BspPI GGATC 5 cut(s) 1014, 1253, 1336, 2171, 2248
BspT107I GGYRCC 1 cut(s) 352
BspTI CTTAAG 1 cut(s) 1766
BspTNI GGTCTC 1 cut(s) 2006
BsrDI GCAATG 3 cut(s) 601, 1282, 1560
BsrFI RCCGGY 5 cut(s) 201, 349, 1214, 1308, 1943
BsrI ACTGG 2 cut(s) 904, 1187
BssAI RCCGGY 5 cut(s) 201, 349, 1214, 1308, 1943
BssECI CCNNGG 8 cut(s) 396, 402, 450, 570, 607, 1749, 1852, 2219
BssNAI GTATAC 1 cut(s) 831
BssNI GRCGYC 4 cut(s) 221, 255, 353, 1930
BssT1I CCWWGG 2 cut(s) 570, 607
Bst1107I GTATAC 1 cut(s) 831
Bst2UI CCWGG 5 cut(s) 1296, 1412, 1750, 1853, 2553
Bst6I CTCTTC 2 cut(s) 1457, 2222
BstACI GRCGYC 4 cut(s) 221, 255, 353, 1930
BstAFI CTTAAG 1 cut(s) 1766
BstAPI GCANNNNNTGC 2 cut(s) 769, 1664
BstDEI CTNAG 8 cut(s) 834, 1070, 1161, 1497, 1994, 2003, 2048, 2484
BstDSI CCRYGG 2 cut(s) 396, 607
BstENI CCTNNNNNAGG 1 cut(s) 449
BstF5I GGATG 3 cut(s) 160, 1809, 2058
BstFNI CGCG 6 cut(s) 198, 218, 416, 546, 934, 2364
BstH2I RGCGCY 1 cut(s) 356
BstHHI GCGC 6 cut(s) 198, 355, 418, 546, 673, 934
BstMAI GTCTC 7 cut(s) 162, 281, 960, 1110, 2006, 2616, 2689
BstMCI CGRYCG 4 cut(s) 202, 402, 426, 438
BstNI CCWGG 5 cut(s) 1296, 1412, 1750, 1853, 2553
BstNSI RCATGY 1 cut(s) 1729
BstSCI CCNGG 6 cut(s) 425, 1294, 1410, 1748, 1851, 2551
BstSFI CTRYAG 2 cut(s) 1757, 2187
BstSLI GKGCMC 3 cut(s) 977, 1442, 1583
BstUI CGCG 6 cut(s) 198, 218, 416, 546, 934, 2364
BstV2I GAAGAC 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 1585
BstYI RGATCY 1 cut(s) 1585
BstZ17I GTATAC 1 cut(s) 831
BstZI CGGCCG 4 cut(s) 199, 399, 423, 435
Bsu15I ATCGAT 1 cut(s) 462
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 2 cut(s) 396, 607
BtrI CACGTC 1 cut(s) 2722
BtsCI GGATG 3 cut(s) 160, 1809, 2058
BtsIMutI CAGTG 2 cut(s) 1440, 2677
BveI ACCTGC 3 cut(s) 490, 1126, 1688
CaiI CAGNNNCTG 1 cut(s) 1667
CciI TCATGA 2 cut(s) 1009, 2233
CfoI GCGC 6 cut(s) 198, 355, 418, 546, 673, 934
Cfr10I RCCGGY 5 cut(s) 201, 349, 1214, 1308, 1943
Cfr13I GGNCC 7 cut(s) 178, 406, 1249, 1279, 1470, 1673, 2447
ClaI ATCGAT 1 cut(s) 462
CseI GACGC 3 cut(s) 244, 1919, 2598
CsiI ACCWGGT 1 cut(s) 1294
Csp6I GTAC 5 cut(s) 903, 946, 1391, 1824, 2369
CviAII CATG 9 cut(s) 608, 647, 1010, 1568, 1726, 1982, 2234, 2321, 2733
CviQI GTAC 5 cut(s) 903, 946, 1391, 1824, 2369
DdeI CTNAG 8 cut(s) 834, 1070, 1161, 1497, 1994, 2003, 2048, 2484
DinI GGCGCC 1 cut(s) 354
DraIII CACNNNGTG 1 cut(s) 328
DriI GACNNNNNGTC 1 cut(s) 34
EaeI YGGCCR 6 cut(s) 199, 399, 423, 435, 1306, 1600
EagI CGGCCG 4 cut(s) 199, 399, 423, 435
Eam1104I CTCTTC 2 cut(s) 1457, 2222
Eam1105I GACNNNNNGTC 1 cut(s) 34
EarI CTCTTC 2 cut(s) 1457, 2222
Ecl136II GAGCTC 2 cut(s) 2045, 2359
EclXI CGGCCG 4 cut(s) 199, 399, 423, 435
Eco130I CCWWGG 2 cut(s) 570, 607
Eco24I GRGCYC 3 cut(s) 1809, 2047, 2361
Eco31I GGTCTC 1 cut(s) 2006
Eco32I GATATC 1 cut(s) 1967
Eco47I GGWCC 5 cut(s) 178, 1249, 1279, 1470, 1673
Eco52I CGGCCG 4 cut(s) 199, 399, 423, 435
Eco53kI GAGCTC 2 cut(s) 2045, 2359
Eco57I CTGAAG 3 cut(s) 1831, 2273, 2436
Eco88I CYCGRG 1 cut(s) 2040
EcoICRI GAGCTC 2 cut(s) 2045, 2359
EcoNI CCTNNNNNAGG 1 cut(s) 449
EcoO109I RGGNCCY 2 cut(s) 406, 2447
EcoRI GAATTC 2 cut(s) 17, 1634
EcoRII CCWGG 5 cut(s) 1294, 1410, 1748, 1851, 2551
EcoRV GATATC 1 cut(s) 1967
EcoT14I CCWWGG 2 cut(s) 570, 607
EcoT22I ATGCAT 1 cut(s) 1983
EcoT38I GRGCYC 3 cut(s) 1809, 2047, 2361
EgeI GGCGCC 1 cut(s) 354
EheI GGCGCC 1 cut(s) 354
ErhI CCWWGG 2 cut(s) 570, 607
Esp3I CGTCTC 2 cut(s) 162, 2616
FaeI CATG 9 cut(s) 611, 650, 1013, 1571, 1729, 1985, 2237, 2324, 2736
FaqI GGGAC 7 cut(s) 53, 164, 198, 1262, 1292, 1784, 2548
FatI CATG 9 cut(s) 607, 646, 1009, 1567, 1725, 1981, 2233, 2320, 2732
FauI CCCGC 6 cut(s) 223, 808, 812, 924, 984, 1978
FbaI TGATCA 3 cut(s) 381, 720, 2251
FblI GTMKAC 2 cut(s) 830, 2186
FokI GGATG 3 cut(s) 147, 1796, 2065
FriOI GRGCYC 3 cut(s) 1809, 2047, 2361
FseI GGCCGGCC 1 cut(s) 205
FspBI CTAG 5 cut(s) 150, 339, 705, 1451, 2340
GlaI GCGC 6 cut(s) 197, 354, 417, 545, 672, 933
GsaI CCCAGC 2 cut(s) 1529, 2535
GsuI CTGGAG 1 cut(s) 2671
HaeII RGCGCY 1 cut(s) 356
HapII CCGG 8 cut(s) 202, 350, 356, 426, 434, 1215, 1309, 1944
HgaI GACGC 3 cut(s) 244, 1919, 2598
HhaI GCGC 6 cut(s) 198, 355, 418, 546, 673, 934
Hin1I GRCGYC 4 cut(s) 221, 255, 353, 1930
Hin1II CATG 9 cut(s) 611, 650, 1013, 1571, 1729, 1985, 2237, 2324, 2736
Hin6I GCGC 6 cut(s) 196, 353, 416, 544, 671, 932
HinP1I GCGC 6 cut(s) 196, 353, 416, 544, 671, 932
HincII GTYRAC 1 cut(s) 2062
HindII GTYRAC 1 cut(s) 2062
HindIII AAGCTT 3 cut(s) 1505, 2411, 2423
HpaI GTTAAC 1 cut(s) 2062
HpaII CCGG 8 cut(s) 202, 350, 356, 426, 434, 1215, 1309, 1944
Hpy99I CGWCG 6 cut(s) 29, 68, 223, 226, 371, 1629
HpyAV CCTTC 9 cut(s) 1018, 1606, 1855, 2050, 2111, 2231, 2460, 2521, 2574
HpyCH4IV ACGT 4 cut(s) 221, 905, 1127, 2721
HpyF3I CTNAG 8 cut(s) 834, 1070, 1161, 1497, 1994, 2003, 2048, 2484
HpySE526I ACGT 4 cut(s) 221, 905, 1127, 2721
Hsp92I GRCGYC 4 cut(s) 221, 255, 353, 1930
Hsp92II CATG 9 cut(s) 611, 650, 1013, 1571, 1729, 1985, 2237, 2324, 2736
HspAI GCGC 6 cut(s) 196, 353, 416, 544, 671, 932
KasI GGCGCC 1 cut(s) 352
KroI GCCGGC 2 cut(s) 201, 1308
KroNI GCCGGC 2 cut(s) 203, 1310
Ksp22I TGATCA 3 cut(s) 381, 720, 2251
KspAI GTTAAC 1 cut(s) 2062
LmnI GCTCC 2 cut(s) 1985, 2364
LweI GCATC 4 cut(s) 759, 1195, 1729, 1892
MabI ACCWGGT 1 cut(s) 1294
MaeI CTAG 5 cut(s) 150, 339, 705, 1451, 2340
MaeII ACGT 4 cut(s) 221, 905, 1127, 2721
MboII GAAGA 9 cut(s) 155, 196, 276, 279, 700, 1474, 1630, 2236, 2239
MfeI CAATTG 1 cut(s) 559
MflI RGATCY 1 cut(s) 1585
MhlI GDGCHC 6 cut(s) 977, 1442, 1583, 1809, 2047, 2361
Mly113I GGCGCC 1 cut(s) 353
MlyI GAGTC 2 cut(s) 401, 2239
MmeI TCCRAC 3 cut(s) 137, 390, 1900
Mph1103I ATGCAT 1 cut(s) 1983
MroNI GCCGGC 2 cut(s) 201, 1308
MroXI GAANNNNTTC 3 cut(s) 17, 1175, 2146
MslI CAYNNNNRTG 3 cut(s) 581, 606, 2091
MspA1I CMGCKG 2 cut(s) 875, 2456
MspCI CTTAAG 1 cut(s) 1766
MspI CCGG 8 cut(s) 202, 350, 356, 426, 434, 1215, 1309, 1944
MspR9I CCNGG 6 cut(s) 427, 1296, 1412, 1750, 1853, 2553
MunI CAATTG 1 cut(s) 559
Mva1269I GAATGC 3 cut(s) 1153, 1162, 2086
MvaI CCWGG 5 cut(s) 1296, 1412, 1750, 1853, 2553
MvnI CGCG 6 cut(s) 198, 218, 416, 546, 934, 2364
NaeI GCCGGC 2 cut(s) 203, 1310
NarI GGCGCC 1 cut(s) 353
NciI CCSGG 1 cut(s) 427
NcoI CCATGG 1 cut(s) 607
NgoMIV GCCGGC 2 cut(s) 201, 1308
NlaIII CATG 9 cut(s) 611, 650, 1013, 1571, 1729, 1985, 2237, 2324, 2736
NlaIV GGNNCC 9 cut(s) 180, 354, 524, 914, 1250, 1280, 1471, 2069, 2448
NmeAIII GCCGAG 3 cut(s) 401, 427, 738
NmuCI GTSAC 7 cut(s) 787, 859, 1382, 1744, 1838, 2203, 2292
NsiI ATGCAT 1 cut(s) 1983
NspI RCATGY 1 cut(s) 1729
PaeI GCATGC 1 cut(s) 1729
PaeR7I CTCGAG 1 cut(s) 2040
PagI TCATGA 2 cut(s) 1009, 2233
PaqCI CACCTGC 2 cut(s) 490, 1688
PcsI WCGNNNNNNNCGW 3 cut(s) 21, 63, 375
PctI GAATGC 3 cut(s) 1153, 1162, 2086
PdiI GCCGGC 2 cut(s) 203, 1310
PdmI GAANNNNTTC 3 cut(s) 17, 1175, 2146
PfeI GAWTC 8 cut(s) 4, 440, 620, 691, 1396, 1616, 1762, 2459
PleI GAGTC 2 cut(s) 400, 2238
PluTI GGCGCC 1 cut(s) 356
PpsI GAGTC 2 cut(s) 400, 2238
PshBI ATTAAT 1 cut(s) 2604
PsiI TTATAA 2 cut(s) 630, 984
Psp124BI GAGCTC 2 cut(s) 2047, 2361
Psp1406I AACGTT 1 cut(s) 1127
Psp6I CCWGG 5 cut(s) 1294, 1410, 1748, 1851, 2551
PspFI CCCAGC 2 cut(s) 1525, 2531
PspGI CCWGG 5 cut(s) 1294, 1410, 1748, 1851, 2551
PspN4I GGNNCC 9 cut(s) 180, 354, 524, 914, 1250, 1280, 1471, 2069, 2448
PspPI GGNCC 7 cut(s) 178, 406, 1249, 1279, 1470, 1673, 2447
PspXI VCTCGAGB 1 cut(s) 2040
PstI CTGCAG 1 cut(s) 1761
PstNI CAGNNNCTG 1 cut(s) 1667
PsuI RGATCY 1 cut(s) 1585
PvuII CAGCTG 1 cut(s) 2456
RigI GGCCGGCC 1 cut(s) 205
RsaI GTAC 5 cut(s) 904, 947, 1392, 1825, 2370
RsaNI GTAC 5 cut(s) 903, 946, 1391, 1824, 2369
RseI CAYNNNNRTG 3 cut(s) 581, 606, 2091
SacI GAGCTC 2 cut(s) 2047, 2361
Sau96I GGNCC 7 cut(s) 178, 406, 1249, 1279, 1470, 1673, 2447
SchI GAGTC 2 cut(s) 401, 2239
ScrFI CCNGG 6 cut(s) 427, 1296, 1412, 1750, 1853, 2553
SduI GDGCHC 6 cut(s) 977, 1442, 1583, 1809, 2047, 2361
SexAI ACCWGGT 1 cut(s) 1294
SfaNI GCATC 4 cut(s) 759, 1195, 1729, 1892
SfcI CTRYAG 2 cut(s) 1757, 2187
SfoI GGCGCC 1 cut(s) 354
Sfr274I CTCGAG 1 cut(s) 2040
SinI GGWCC 5 cut(s) 178, 1249, 1279, 1470, 1673
SlaI CTCGAG 1 cut(s) 2040
SmiMI CAYNNNNRTG 3 cut(s) 581, 606, 2091
SmlI CTYRAG 3 cut(s) 1766, 2040, 2354
SmoI CTYRAG 3 cut(s) 1766, 2040, 2354
SphI GCATGC 1 cut(s) 1729
SspDI GGCGCC 1 cut(s) 352
SspMI CTAG 5 cut(s) 150, 339, 705, 1451, 2340
SstI GAGCTC 2 cut(s) 2047, 2361
StyD4I CCNGG 6 cut(s) 425, 1294, 1410, 1748, 1851, 2551
StyI CCWWGG 2 cut(s) 570, 607
TaiI ACGT 4 cut(s) 224, 908, 1130, 2724
TaqI TCGA 7 cut(s) 141, 314, 344, 462, 1627, 2041, 2540
TauI GCSGC 5 cut(s) 124, 201, 255, 937, 2015
TfiI GAWTC 8 cut(s) 4, 440, 620, 691, 1396, 1616, 1762, 2459
TscAI CASTG 2 cut(s) 1447, 2677
TseFI GTSAC 7 cut(s) 787, 859, 1382, 1744, 1838, 2203, 2292
Tsp45I GTSAC 7 cut(s) 787, 859, 1382, 1744, 1838, 2203, 2292
TspDTI ATGAA 8 cut(s) 17, 944, 1037, 1184, 1794, 2069, 2250, 2309
TspGWI ACGGA 1 cut(s) 1251
TspRI CASTG 2 cut(s) 1447, 2677
Vha464I CTTAAG 1 cut(s) 1766
VneI GTGCAC 1 cut(s) 1438
VpaK11BI GGWCC 5 cut(s) 178, 1249, 1279, 1470, 1673
VspI ATTAAT 1 cut(s) 2604
XagI CCTNNNNNAGG 1 cut(s) 449
XapI RAATTY 4 cut(s) 17, 1195, 1634, 2135
XceI RCATGY 1 cut(s) 1729
XhoI CTCGAG 1 cut(s) 2040
XmiI GTMKAC 2 cut(s) 830, 2186
XmnI GAANNNNTTC 3 cut(s) 17, 1175, 2146
XspI CTAG 5 cut(s) 150, 339, 705, 1451, 2340
ZraI GACGTC 1 cut(s) 222
Zsp2I ATGCAT 1 cut(s) 1983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.