AT3G12640

PWI domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006139
Physical Location & Seq
Forward (+)
4014047 .. 4017869
3823 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12640.2

Sequence Viewer

Length: 1917 bp
ATGGGAAGCGCCGATCTAGTCGATGATCGGACCTTTAACGGTGATTTCAGTGTGGAAGGAGTTGCTAAGTTGAAAGAGATGGTCAAGGATAAACTCAAGGAGTATATGGGCGATTACACGGATGACATTCTTGTGGAATATGTGATCGTCTTGCTTAGAAATGGGAGAAGAAAAGAGGAAGCAAATAATGAATTGAAGATATTTCTTGGAGATGATAGCGACTCTTTTGTGGCCTGGTTGTGGGATCATCTGGCTGAAAGTGTAGATGAGTACTTTAGTTCTAATGTGGAGGGAACTACCATAAAAAGCTCATTGATAAGCAGTCATGAGGATAAAGCACTTGTTCCTCTGGATTCTGAATATGATAAGGGACGATCTGATAAATCAAATGGTGCACGTCGTGGTAGACAATGGAGAAGCCAGCCGACTAATGTCTCTGAGATCCCTCCTCTTCTGAGCTCTGAGGCTCAATCTCAGAGGAAAAGGTCTAGAACTGACGACTCACGGAATGAACAGAGGGAAGCTAAACCTGATGTCTCTCGCCGGCTGCTTCAGTTTGCTGTGCGAGATGCTCTAGCTATATCAAGGCCGGCAAATAGTTCGACTGAATCCTCATTAAAACGTCTTCGTTCAGTGGTGTCCACATCTACTCAAAACTCATCTGACCCTGATCCAGCTCGGAAAATTCGATCTGTTGCTAGGGTTGTGAATCCCATGGCTACTGTTATGAAAGCTGTCGCCGAAGCAGCCGAAGATGCCAAGAAACCAAAATCTGGGAGAAGTGTCTTTGACAGAATTAGTCATTCTACTGGATCAGAGACCCTGGACCAGAATATGGTACTTGGTGAAGTTTCCCCCAAGGATGAAGAATCTAGGAATTTTAGCCAAGGTCAGGAAGCGGTACAGCTTCAATACACACAGAGTCTTGCCAATAATGGAGTGTATTTTGAGAACATGACAACTAATGACACTGGCTTGAATCCAGATTTTTCGTCTGATCGAGGCAGACTCGGTTCAAGTGTTAATGTGTCTCACCCAAGTACTTATCTTGGGAACAGAATTAACAATCCAAATAGTCTGCAGCATCGTTTAGTCGATGACTCCAAACGAGTTAAAGGGACAAATTATCAAAATCGCCTTCCTGAAGTTGCAACCAAGCATAAGACTGCAAGTTTCTCAGGCAATATAGATACAGGGAAAACAGTAAAGTTAGAGGAGCAGAGGAAAATACCAGATGTGGGTCTGCAAAGATATATGGACGAAGGCAGATTAGTTTCTAGTGAAGCTACCACTCAATTATCGACGCAGAAAATCTTGGGTGATACAATAGGGAATGGAAATGTAAAACCTGCTGCCAGTGTGAAAGAAGAATCAACGACCAATAAATCTGTTCCTGGGACATTATCCACTACCCGCCCTTTGGAAGATGCTAGTTCTCGAACAATCTTTGTCGCCAATGTTCATTTTGGTGCCACCAAGGACAGCCTTTCAAGGCATTTTAACAAGTTTGGTGAAGTGCTTAAAGCTTTCATAGTTACTGATCCAGCAACAGGACAACCAAGTGGATCAGCGTACATTGAGTTCACACGCAAAGAAGCTGCAGAGAATGCATTGTCTCTGGATGGTACCTCTTTCATGTCTCGGATTCTCAAGATTGTGAAAGGAAGCAATGGACAAAATCAAGAGGCTGCATCGAGCATGTCATGGTCTCGAGGTGGAAGGTTCACAAGAGCCCCATCATACTTTAGAGGTGGTGCAGTTAGGGGTCGTTCTGTTGTTAGAGGTGGAGGCAGGAGTATGCAATGGAAGCGTGATTCAGCTGACACAGGAAACAACAACAATGTAGCTCCCAACAATGCTCGTAGTCTCACGTATGTCAGAGCAGAATCAAAATCAGACGGAGTTGCCAACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008143 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009892 GO:0009894 GO:0009987 GO:0010033 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016234 GO:0016310 GO:0016604 GO:0016607 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023052 GO:0031323 GO:0031324 GO:0031329 GO:0031440 GO:0031441 GO:0031974 GO:0031981 GO:0032496 GO:0032991 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042405 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043487 GO:0043488 GO:0043621 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045934 GO:0048471 GO:0048519 GO:0048523 GO:0050684 GO:0050686 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061013 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070063 GO:0070717 GO:0070887 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071396 GO:0071704 GO:0080090 GO:0097159 GO:1900363 GO:1900364 GO:1901363 GO:1901564 GO:1901700 GO:1901701 GO:1903311 GO:1903312 GO:1904245 GO:1904247 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWI RRM_1

Protein Analysis

638

Amino Acids

70.1

Weight (kDa)

9.11

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWI PF01480 23 - 86 3.5e-08 PWI domain
RRM_1 PF00076 506 - 574 1.2e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1357
Acc65I GGTACC 1 cut(s) 1625
AccB1I GGYRCC 2 cut(s) 1469, 1625
AccB7I CCANNNNNTGG 2 cut(s) 773, 835
AccI GTMKAC 1 cut(s) 406
AciI CCGC 2 cut(s) 899, 1414
AclWI GGATC 6 cut(s) 252, 436, 665, 820, 1535, 1573
AcsI RAATTY 2 cut(s) 684, 877
AcuI CTGAAG 2 cut(s) 536, 1164
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 6 cut(s) 272, 840, 903, 1042, 1574, 1627
AfiI CCNNNNNNNGG 7 cut(s) 240, 773, 835, 892, 1238, 1420, 1550
AgsI TTSAA 6 cut(s) 73, 196, 911, 979, 1017, 1491
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 3 cut(s) 233, 822, 1393
Alw21I GWGCWC 2 cut(s) 397, 461
Alw26I GTCTC 8 cut(s) 439, 541, 812, 1035, 1620, 1644, 1713, 1871
Alw44I GTGCAC 1 cut(s) 393
AlwI GGATC 6 cut(s) 252, 436, 665, 820, 1535, 1573
Ama87I CYCGRG 1 cut(s) 1710
AoxI GGCC 2 cut(s) 231, 587
ApaLI GTGCAC 1 cut(s) 393
ApeKI GCWGC 6 cut(s) 547, 746, 1081, 1352, 1598, 1688
ApoI RAATTY 2 cut(s) 684, 877
Asp718I GGTACC 1 cut(s) 1625
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 30, 826
AsuHPI GGTGA 5 cut(s) 53, 857, 1025, 1331, 1523
AvaI CYCGRG 1 cut(s) 1710
AvaII GGWCC 2 cut(s) 30, 826
BaeGI GKGCMC 1 cut(s) 397
BanI GGYRCC 2 cut(s) 1469, 1625
BanII GRGCYC 2 cut(s) 461, 1735
BbsI GAAGAC 1 cut(s) 617
Bbv12I GWGCWC 2 cut(s) 397, 461
BbvI GCAGC 6 cut(s) 534, 758, 1093, 1339, 1585, 1675
BccI CCATC 3 cut(s) 73, 1616, 1744
BcgI CGANNNNNNTGC 2 cut(s) 582, 616
BciT130I CCWGG 3 cut(s) 235, 824, 1395
BcoDI GTCTC 8 cut(s) 439, 541, 812, 1035, 1620, 1644, 1713, 1871
BfaI CTAG 7 cut(s) 17, 489, 575, 699, 873, 1278, 1431
BfmI CTRYAG 2 cut(s) 1079, 1599
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 1357
BisI GCNGC 6 cut(s) 548, 747, 1082, 1353, 1599, 1689
BlsI GCNGC 6 cut(s) 549, 748, 1083, 1354, 1600, 1690
BmcAI AGTACT 2 cut(s) 272, 1042
Bme1390I CCNGG 3 cut(s) 235, 824, 1395
Bme18I GGWCC 2 cut(s) 30, 826
BmeT110I CYCGRG 1 cut(s) 1710
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 2 cut(s) 30, 826
BmiI GGNNCC 2 cut(s) 1471, 1627
BmrFI CCNGG 3 cut(s) 235, 824, 1395
BmsI GCATC 5 cut(s) 559, 745, 1093, 1417, 1700
BoxI GACNNNNGTC 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 617
BplI GAGNNNNNCTC 2 cut(s) 993, 1025
BpuEI CTTGAG 2 cut(s) 80, 1634
BsaAI YACGTR 1 cut(s) 1872
BsaI GGTCTC 2 cut(s) 812, 1713
BsaJI CCNNGG 6 cut(s) 714, 822, 858, 886, 1394, 1476
Bsc4I CCNNNNNNNGG 7 cut(s) 240, 773, 835, 892, 1238, 1420, 1550
Bse118I RCCGGY 2 cut(s) 543, 589
Bse1I ACTGG 3 cut(s) 814, 976, 1356
Bse3DI GCAATG 2 cut(s) 1675, 1808
BseBI CCWGG 3 cut(s) 235, 824, 1395
BseDI CCNNGG 6 cut(s) 714, 822, 858, 886, 1394, 1476
BseGI GGATG 3 cut(s) 127, 868, 1627
BseLI CCNNNNNNNGG 7 cut(s) 240, 773, 835, 892, 1238, 1420, 1550
BseMI GCAATG 2 cut(s) 1675, 1808
BseMII CTCAG 5 cut(s) 429, 446, 453, 488, 1191
BseNI ACTGG 3 cut(s) 814, 976, 1356
BseRI GAGGAG 2 cut(s) 438, 1229
BseSI GKGCMC 1 cut(s) 397
BseXI GCAGC 6 cut(s) 534, 758, 1093, 1339, 1585, 1675
BsgI GTGCAG 1 cut(s) 1776
BshFI GGCC 2 cut(s) 233, 589
BshNI GGYRCC 2 cut(s) 1469, 1625
BsiHKAI GWGCWC 2 cut(s) 397, 461
BsiHKCI CYCGRG 1 cut(s) 1710
BsiSI CCGG 2 cut(s) 544, 590
BslFI GGGAC 3 cut(s) 384, 1132, 1411
BslI CCNNNNNNNGG 7 cut(s) 240, 773, 835, 892, 1238, 1420, 1550
BsmAI GTCTC 8 cut(s) 439, 541, 812, 1035, 1620, 1644, 1713, 1871
BsmFI GGGAC 3 cut(s) 384, 1132, 1411
BsmI GAATGC 1 cut(s) 1612
BsnI GGCC 2 cut(s) 233, 589
Bso31I GGTCTC 2 cut(s) 812, 1713
BsoBI CYCGRG 1 cut(s) 1710
Bsp1286I GDGCHC 3 cut(s) 397, 461, 1735
Bsp19I CCATGG 1 cut(s) 714
BspACI CCGC 2 cut(s) 899, 1414
BspANI GGCC 2 cut(s) 233, 589
BspCNI CTCAG 5 cut(s) 430, 447, 454, 487, 1190
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 2 cut(s) 1471, 1627
BspMAI CTGCAG 2 cut(s) 1083, 1603
BspMI ACCTGC 1 cut(s) 1357
BspPI GGATC 6 cut(s) 252, 436, 665, 820, 1535, 1573
BspT107I GGYRCC 2 cut(s) 1469, 1625
BspTNI GGTCTC 2 cut(s) 812, 1713
BsrDI GCAATG 2 cut(s) 1675, 1808
BsrFI RCCGGY 2 cut(s) 543, 589
BsrI ACTGG 3 cut(s) 814, 976, 1356
BssAI RCCGGY 2 cut(s) 543, 589
BssECI CCNNGG 6 cut(s) 714, 822, 858, 886, 1394, 1476
BssT1I CCWWGG 4 cut(s) 714, 858, 886, 1476
Bst2UI CCWGG 3 cut(s) 235, 824, 1395
Bst4CI ACNGT 3 cut(s) 41, 724, 1204
Bst6I CTCTTC 1 cut(s) 456
BstAPI GCANNNNNTGC 1 cut(s) 1607
BstBAI YACGTR 1 cut(s) 1872
BstC8I GCNNGC 3 cut(s) 422, 545, 591
BstDEI CTNAG 7 cut(s) 66, 155, 438, 455, 462, 474, 1177
BstDSI CCRYGG 1 cut(s) 714
BstF5I GGATG 3 cut(s) 127, 868, 1627
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstMAI GTCTC 8 cut(s) 439, 541, 812, 1035, 1620, 1644, 1713, 1871
BstMWI GCNNNNNNNGC 4 cut(s) 746, 755, 1607, 1807
BstNI CCWGG 3 cut(s) 235, 824, 1395
BstNSI RCATGY 1 cut(s) 1702
BstPAI GACNNNNGTC 1 cut(s) 431
BstSCI CCNGG 3 cut(s) 233, 822, 1393
BstSFI CTRYAG 2 cut(s) 1079, 1599
BstSLI GKGCMC 1 cut(s) 397
BstV1I GCAGC 6 cut(s) 534, 758, 1093, 1339, 1585, 1675
BstV2I GAAGAC 1 cut(s) 617
BstX2I RGATCY 1 cut(s) 441
BstYI RGATCY 1 cut(s) 441
BsuRI GGCC 2 cut(s) 233, 589
BtgI CCRYGG 1 cut(s) 714
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 3 cut(s) 127, 868, 1627
BtsIMutI CAGTG 4 cut(s) 55, 639, 969, 1363
BveI ACCTGC 1 cut(s) 1357
Cac8I GCNNGC 3 cut(s) 422, 545, 591
CciI TCATGA 1 cut(s) 325
CfoI GCGC 1 cut(s) 11
Cfr10I RCCGGY 2 cut(s) 543, 589
Cfr13I GGNCC 2 cut(s) 30, 826
CseI GACGC 1 cut(s) 1312
Csp6I GTAC 6 cut(s) 271, 839, 902, 1041, 1573, 1626
CviAII CATG 6 cut(s) 326, 715, 955, 1636, 1699, 1704
CviQI GTAC 6 cut(s) 271, 839, 902, 1041, 1573, 1626
DdeI CTNAG 7 cut(s) 66, 155, 438, 455, 462, 474, 1177
DraIII CACNNNGTG 1 cut(s) 401
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Ecl136II GAGCTC 1 cut(s) 459
Eco130I CCWWGG 4 cut(s) 714, 858, 886, 1476
Eco24I GRGCYC 2 cut(s) 461, 1735
Eco31I GGTCTC 2 cut(s) 812, 1713
Eco47I GGWCC 2 cut(s) 30, 826
Eco53kI GAGCTC 1 cut(s) 459
Eco57I CTGAAG 2 cut(s) 536, 1164
Eco88I CYCGRG 1 cut(s) 1710
EcoICRI GAGCTC 1 cut(s) 459
EcoRII CCWGG 3 cut(s) 233, 822, 1393
EcoT14I CCWWGG 4 cut(s) 714, 858, 886, 1476
EcoT22I ATGCAT 1 cut(s) 1612
EcoT38I GRGCYC 2 cut(s) 461, 1735
ErhI CCWWGG 4 cut(s) 714, 858, 886, 1476
FaeI CATG 6 cut(s) 329, 718, 958, 1639, 1702, 1707
FaqI GGGAC 3 cut(s) 384, 1132, 1411
FatI CATG 6 cut(s) 325, 714, 954, 1635, 1698, 1703
FauI CCCGC 1 cut(s) 1421
FblI GTMKAC 1 cut(s) 406
Fnu4HI GCNGC 6 cut(s) 548, 747, 1082, 1353, 1599, 1689
FokI GGATG 3 cut(s) 134, 875, 1634
FriOI GRGCYC 2 cut(s) 461, 1735
Fsp4HI GCNGC 6 cut(s) 548, 747, 1082, 1353, 1599, 1689
FspBI CTAG 7 cut(s) 17, 489, 575, 699, 873, 1278, 1431
GlaI GCGC 1 cut(s) 10
GluI GCNGC 6 cut(s) 548, 747, 1082, 1353, 1599, 1689
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 233, 589
HapII CCGG 2 cut(s) 544, 590
HgaI GACGC 1 cut(s) 1312
HhaI GCGC 1 cut(s) 11
Hin1II CATG 6 cut(s) 329, 718, 958, 1639, 1702, 1707
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HindIII AAGCTT 1 cut(s) 1524
HpaII CCGG 2 cut(s) 544, 590
HphI GGTGA 5 cut(s) 53, 857, 1025, 1331, 1523
Hpy166II GTNNAC 5 cut(s) 395, 407, 642, 1584, 1725
Hpy8I GTNNAC 5 cut(s) 395, 407, 642, 1584, 1725
Hpy99I CGWCG 2 cut(s) 402, 1306
HpyAV CCTTC 4 cut(s) 50, 1148, 1256, 1713
HpyCH4III ACNGT 3 cut(s) 41, 724, 1204
HpyCH4IV ACGT 3 cut(s) 397, 622, 1871
HpyF10VI GCNNNNNNNGC 4 cut(s) 746, 755, 1607, 1807
HpyF3I CTNAG 7 cut(s) 66, 155, 438, 455, 462, 474, 1177
HpySE526I ACGT 3 cut(s) 397, 622, 1871
Hsp92II CATG 6 cut(s) 329, 718, 958, 1639, 1702, 1707
HspAI GCGC 1 cut(s) 9
KpnI GGTACC 1 cut(s) 1629
KroI GCCGGC 2 cut(s) 543, 589
KroNI GCCGGC 2 cut(s) 545, 591
LmnI GCTCC 2 cut(s) 1216, 1852
Lsp1109I GCAGC 6 cut(s) 534, 758, 1093, 1339, 1585, 1675
LweI GCATC 5 cut(s) 559, 745, 1093, 1417, 1700
MaeI CTAG 7 cut(s) 17, 489, 575, 699, 873, 1278, 1431
MaeII ACGT 3 cut(s) 397, 622, 1871
MaeIII GTNAC 1 cut(s) 1534
MboII GAAGA 8 cut(s) 180, 208, 443, 617, 764, 878, 1379, 1436
MflI RGATCY 1 cut(s) 441
MhlI GDGCHC 3 cut(s) 397, 461, 1735
MluCI AATT 7 cut(s) 191, 684, 795, 877, 1059, 1123, 1295
MlyI GAGTC 5 cut(s) 215, 494, 931, 1002, 1094
Mph1103I ATGCAT 1 cut(s) 1612
MroNI GCCGGC 2 cut(s) 543, 589
MseI TTAA 7 cut(s) 36, 617, 1023, 1062, 1113, 1500, 1521
MslI CAYNNNNRTG 2 cut(s) 131, 1467
MspA1I CMGCKG 1 cut(s) 1820
MspI CCGG 2 cut(s) 544, 590
MspR9I CCNGG 3 cut(s) 235, 824, 1395
Mva1269I GAATGC 1 cut(s) 1612
MvaI CCWGG 3 cut(s) 235, 824, 1395
MwoI GCNNNNNNNGC 4 cut(s) 746, 755, 1607, 1807
NaeI GCCGGC 2 cut(s) 545, 591
NcoI CCATGG 1 cut(s) 714
NgoMIV GCCGGC 2 cut(s) 543, 589
NlaIII CATG 6 cut(s) 329, 718, 958, 1639, 1702, 1707
NlaIV GGNNCC 2 cut(s) 1471, 1627
NsiI ATGCAT 1 cut(s) 1612
NspI RCATGY 1 cut(s) 1702
PaeR7I CTCGAG 1 cut(s) 1710
PagI TCATGA 1 cut(s) 325
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PctI GAATGC 1 cut(s) 1612
PdiI GCCGGC 2 cut(s) 545, 591
PfeI GAWTC 9 cut(s) 353, 608, 709, 869, 979, 1370, 1645, 1814, 1886
PflMI CCANNNNNTGG 2 cut(s) 773, 835
PkrI GCNGC 6 cut(s) 549, 748, 1083, 1354, 1600, 1690
PleI GAGTC 5 cut(s) 215, 494, 930, 1002, 1094
PpsI GAGTC 5 cut(s) 215, 494, 930, 1002, 1094
Ppu21I YACGTR 1 cut(s) 1872
PshAI GACNNNNGTC 1 cut(s) 431
Psp124BI GAGCTC 1 cut(s) 461
Psp6I CCWGG 3 cut(s) 233, 822, 1393
PspGI CCWGG 3 cut(s) 233, 822, 1393
PspN4I GGNNCC 2 cut(s) 1471, 1627
PspPI GGNCC 2 cut(s) 30, 826
PsrI GAACNNNNNNTAC 2 cut(s) 1565, 1597
PstI CTGCAG 2 cut(s) 1083, 1603
PsuI RGATCY 1 cut(s) 441
PvuII CAGCTG 1 cut(s) 1820
RsaI GTAC 6 cut(s) 272, 840, 903, 1042, 1574, 1627
RsaNI GTAC 6 cut(s) 271, 839, 902, 1041, 1573, 1626
RseI CAYNNNNRTG 2 cut(s) 131, 1467
SacI GAGCTC 1 cut(s) 461
SaqAI TTAA 7 cut(s) 36, 617, 1023, 1062, 1113, 1500, 1521
SatI GCNGC 6 cut(s) 548, 747, 1082, 1353, 1599, 1689
Sau96I GGNCC 2 cut(s) 30, 826
ScaI AGTACT 2 cut(s) 272, 1042
SchI GAGTC 5 cut(s) 215, 494, 931, 1002, 1094
ScrFI CCNGG 3 cut(s) 235, 824, 1395
SduI GDGCHC 3 cut(s) 397, 461, 1735
SfaNI GCATC 5 cut(s) 559, 745, 1093, 1417, 1700
SfcI CTRYAG 2 cut(s) 1079, 1599
Sfr274I CTCGAG 1 cut(s) 1710
SinI GGWCC 2 cut(s) 30, 826
SlaI CTCGAG 1 cut(s) 1710
SmiMI CAYNNNNRTG 2 cut(s) 131, 1467
SmlI CTYRAG 3 cut(s) 95, 1649, 1710
SmoI CTYRAG 3 cut(s) 95, 1649, 1710
Sse9I AATT 7 cut(s) 191, 684, 795, 877, 1059, 1123, 1295
SsiI CCGC 2 cut(s) 899, 1414
SspMI CTAG 7 cut(s) 17, 489, 575, 699, 873, 1278, 1431
SstI GAGCTC 1 cut(s) 461
StyD4I CCNGG 3 cut(s) 233, 822, 1393
StyI CCWWGG 4 cut(s) 714, 858, 886, 1476
TaaI ACNGT 3 cut(s) 41, 724, 1204
TaiI ACGT 3 cut(s) 400, 625, 1874
TaqI TCGA 9 cut(s) 21, 602, 688, 1000, 1095, 1301, 1438, 1694, 1711
TasI AATT 7 cut(s) 191, 684, 795, 877, 1059, 1123, 1295
TatI WGTACW 2 cut(s) 270, 1040
TfiI GAWTC 9 cut(s) 353, 608, 709, 869, 979, 1370, 1645, 1814, 1886
Tru1I TTAA 7 cut(s) 36, 617, 1023, 1062, 1113, 1500, 1521
Tru9I TTAA 7 cut(s) 36, 617, 1023, 1062, 1113, 1500, 1521
TscAI CASTG 4 cut(s) 55, 639, 976, 1363
TseI GCWGC 6 cut(s) 547, 746, 1081, 1352, 1598, 1688
TspDTI ATGAA 7 cut(s) 204, 525, 743, 879, 1451, 1519, 1624
TspGWI ACGGA 3 cut(s) 134, 520, 1914
TspRI CASTG 4 cut(s) 55, 639, 976, 1363
Van91I CCANNNNNTGG 2 cut(s) 773, 835
VneI GTGCAC 1 cut(s) 393
VpaK11BI GGWCC 2 cut(s) 30, 826
XapI RAATTY 2 cut(s) 684, 877
XbaI TCTAGA 1 cut(s) 488
XceI RCATGY 1 cut(s) 1702
XhoI CTCGAG 1 cut(s) 1710
XmiI GTMKAC 1 cut(s) 406
XspI CTAG 7 cut(s) 17, 489, 575, 699, 873, 1278, 1431
ZrmI AGTACT 2 cut(s) 272, 1042
Zsp2I ATGCAT 1 cut(s) 1612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.