AT3G12900

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012769
Physical Location & Seq
Forward (+)
4104463 .. 4106341
1879 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12900.1

Sequence Viewer

Length: 1074 bp
ATGGGTATCAATTTCGAGGACCAAACCACATTGTTCAATTTCGTGGTCAGAGAAGGTAACGGCGTGAAAGGAATGATAGACTCGGGCCTCTCTAGTGTTCCACGGCCCTTCGTCCAGCCTCTCTCTGAGCGAATCCCGACCCAAAAAGCCCTTACGTGTGAGGCTACCCAGCCAATCGATCTTTCCAACCTAGACGGCCCACAACACAAGGAGGTGGCCAAGCAGATTGTCGAAGCTGCCGAGACACTTGGCTTCTTCCAGGTGGTGAACCATGGTGTTTCGGTGGAGCTGCTGGAGTTGCTAAAATCGTCGGCTCATGAGTTTTTTGCACAAGCTCCTGAGGAAAAATCGATGTACCTGAAGGAAGTGAGTCCAAGCAAGCTGGTTAAGTACGGAACGAGCTTTGTGCCAGATAAGGAGAAGGCGATTGAGTGGAAGGATTATGTGAGCATGCTTTACACCAATGACAGTGAGGCTCTTCAACACTGGCCTCAACCATGCAGGGAGGTGGCACTTGAGTTCCTAAATTCATCGATGGAAATGGTGAAAAATGTAGTTAACATTCTGATGGAGAATGTTGGAGTGACACTAGAAGAAGAGAAAATGAATGGTTTGATGGGAACCAAGATGGTCAACATGAACTACTACCCTACTTGTCCGAGCCCCGAGCTGACCGTCGGCGTCGGCCGTCACTCAGACATGGGAATGTTGACCGTTTTATTACAAGACGGCATTGGTGGTCTCTACGTTAAACTAGACAACGGTGAATGGGCCGAGATCCCTCCTGTCCATGGAGCTTTGGTCATCAACATCGGCGACACTTTGCAGATTTTGAGCAATGGGAAGTACAAAAGTGCGGAGCATAGGGTTCGAACCACAAACATCGGATCAAGAGTCTCTGTGCCCATTTTCACGGCACCAAATCCCTCCCAGAAGGTCGGTCCCTTGCCGGAAGTTGTGAAACGCGACGGAGTGGCTCGTTACAAGGAGTTTTTGTTTCAAGACTACATGAATAACTTCTTCGGCCAACCACACGACGGCAAAAAATCTCTCGACTTCGCACGTGCCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

357

Amino Acids

39.72

Weight (kDa)

5.33

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 59 - 165 3.3e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 209 - 307 1.3e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12900
fragaria_vesca FvH4_3g15580
malus_domestica MD00G1188900.v1.1 MD05G1221300.v1.1
prunus_persica Prupe.4G138300_v2.0.a1
pyrus_communis pycom05g20470
rosa_chinensis RchiOBHm_Chr5g0026211
rosa_laevigata RLG00000032912
rosa_multiflora Rmu_co8420971.1_g000001 Rmu_sc0002909.1_g000002 Rmu_sc0005061.1_g000001
rosa_roxburghii Rroxscaffold_1G00053100
rosa_rugosa Rorug05G0091600
rosa_samantha Rh5AG184500 Rh5BG181400 Rh5CG200500 Rh5DG183100
rosa_wichuraiana Rw5G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 916
AccII CGCG 1 cut(s) 966
AciI CCGC 1 cut(s) 857
AclWI GGATC 2 cut(s) 772, 895
AcoI YGGCCR 3 cut(s) 216, 685, 1024
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 380
AcvI CACGTG 1 cut(s) 1064
AcyI GRCGYC 1 cut(s) 681
AfaI GTAC 3 cut(s) 356, 392, 848
AfiI CCNNNNNNNGG 2 cut(s) 791, 1037
AflIII ACRYGT 1 cut(s) 155
AgsI TTSAA 3 cut(s) 37, 482, 1001
AjnI CCWGG 1 cut(s) 258
AjuI GAANNNNNNNTTGG 2 cut(s) 166, 198
AluBI AGCT 7 cut(s) 236, 289, 335, 382, 402, 670, 797
AluI AGCT 7 cut(s) 236, 289, 335, 382, 402, 670, 797
Alw26I GTCTC 3 cut(s) 236, 746, 901
AlwI GGATC 2 cut(s) 772, 895
Ama87I CYCGRG 2 cut(s) 82, 665
AoxI GGCC 8 cut(s) 85, 104, 196, 216, 488, 685, 771, 1024
ApeKI GCWGC 2 cut(s) 236, 289
ApoI RAATTY 1 cut(s) 526
Asp700I GAANNNNTTC 1 cut(s) 1016
AspS9I GGNCC 6 cut(s) 19, 85, 105, 197, 771, 941
AsuHPI GGTGA 3 cut(s) 277, 556, 776
AsuII TTCGAA 1 cut(s) 871
AvaI CYCGRG 2 cut(s) 82, 665
AvaII GGWCC 2 cut(s) 19, 941
AxyI CCTNAGG 1 cut(s) 339
BaeGI GKGCMC 1 cut(s) 906
BalI TGGCCA 1 cut(s) 218
BanI GGYRCC 1 cut(s) 916
BanII GRGCYC 1 cut(s) 665
BbrPI CACGTG 1 cut(s) 1064
BbvI GCAGC 2 cut(s) 223, 276
BccI CCATC 4 cut(s) 529, 562, 610, 622
BceAI ACGGC 7 cut(s) 76, 119, 211, 672, 745, 930, 1054
BcgI CGANNNNNNTGC 4 cut(s) 388, 422, 851, 885
BciT130I CCWGG 1 cut(s) 260
BcoDI GTCTC 3 cut(s) 236, 746, 901
BfaI CTAG 4 cut(s) 93, 191, 590, 755
BisI GCNGC 2 cut(s) 237, 290
BlsI GCNGC 2 cut(s) 238, 291
Bme1390I CCNGG 1 cut(s) 260
Bme18I GGWCC 2 cut(s) 19, 941
BmeT110I CYCGRG 2 cut(s) 82, 665
BmgT120I GGNCC 6 cut(s) 19, 85, 105, 197, 771, 941
BmiI GGNNCC 3 cut(s) 622, 918, 943
BmrFI CCNGG 1 cut(s) 260
BpmI CTGGAG 1 cut(s) 314
Bpu14I TTCGAA 1 cut(s) 871
BpuEI CTTGAG 1 cut(s) 536
Bsa29I ATCGAT 3 cut(s) 177, 350, 533
BsaAI YACGTR 2 cut(s) 156, 1064
BsaHI GRCGYC 1 cut(s) 681
BsaI GGTCTC 1 cut(s) 746
BsaJI CCNNGG 3 cut(s) 101, 271, 790
BsaXI ACNNNNNCTCC 4 cut(s) 786, 816, 980, 1010
Bsc4I CCNNNNNNNGG 2 cut(s) 791, 1037
Bse1I ACTGG 1 cut(s) 491
Bse21I CCTNAGG 1 cut(s) 339
Bse3DI GCAATG 1 cut(s) 844
BseBI CCWGG 1 cut(s) 260
BseCI ATCGAT 3 cut(s) 177, 350, 533
BseDI CCNNGG 3 cut(s) 101, 271, 790
BseLI CCNNNNNNNGG 2 cut(s) 791, 1037
BseMI GCAATG 1 cut(s) 844
BseMII CTCAG 3 cut(s) 117, 330, 708
BseNI ACTGG 1 cut(s) 491
BseSI GKGCMC 1 cut(s) 906
BseX3I CGGCCG 1 cut(s) 685
BseXI GCAGC 2 cut(s) 223, 276
BseYI CCCAGC 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 966
Bsh1285I CGRYCG 1 cut(s) 688
BshFI GGCC 8 cut(s) 87, 106, 198, 218, 490, 687, 773, 1026
BshNI GGYRCC 1 cut(s) 916
BshVI ATCGAT 3 cut(s) 177, 350, 533
BsiEI CGRYCG 1 cut(s) 688
BsiHKCI CYCGRG 2 cut(s) 82, 665
BsiSI CCGG 1 cut(s) 950
BslFI GGGAC 1 cut(s) 927
BslI CCNNNNNNNGG 2 cut(s) 791, 1037
BsmAI GTCTC 3 cut(s) 236, 746, 901
BsmFI GGGAC 1 cut(s) 927
BsnI GGCC 8 cut(s) 87, 106, 198, 218, 490, 687, 773, 1026
Bso31I GGTCTC 1 cut(s) 746
BsoBI CYCGRG 2 cut(s) 82, 665
Bsp119I TTCGAA 1 cut(s) 871
Bsp1286I GDGCHC 2 cut(s) 665, 906
Bsp143I GATC 3 cut(s) 178, 777, 887
Bsp19I CCATGG 2 cut(s) 271, 790
BspACI CCGC 1 cut(s) 857
BspANI GGCC 8 cut(s) 87, 106, 198, 218, 490, 687, 773, 1026
BspCNI CTCAG 3 cut(s) 118, 331, 707
BspDI ATCGAT 3 cut(s) 177, 350, 533
BspFNI CGCG 1 cut(s) 966
BspHI TCATGA 1 cut(s) 316
BspLI GGNNCC 3 cut(s) 622, 918, 943
BspPI GGATC 2 cut(s) 772, 895
BspQI GCTCTTC 1 cut(s) 483
BspT104I TTCGAA 1 cut(s) 871
BspT107I GGYRCC 1 cut(s) 916
BspTNI GGTCTC 1 cut(s) 746
BsrDI GCAATG 1 cut(s) 844
BsrI ACTGG 1 cut(s) 491
BssECI CCNNGG 3 cut(s) 101, 271, 790
BssMI GATC 3 cut(s) 178, 777, 887
BssNI GRCGYC 1 cut(s) 681
BssT1I CCWWGG 2 cut(s) 271, 790
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 4 cut(s) 470, 676, 715, 764
Bst6I CTCTTC 2 cut(s) 483, 591
BstACI GRCGYC 1 cut(s) 681
BstBAI YACGTR 2 cut(s) 156, 1064
BstBI TTCGAA 1 cut(s) 871
BstC8I GCNNGC 2 cut(s) 380, 452
BstDEI CTNAG 3 cut(s) 126, 339, 694
BstDSI CCRYGG 3 cut(s) 101, 271, 790
BstFNI CGCG 1 cut(s) 966
BstKTI GATC 3 cut(s) 181, 780, 890
BstMAI GTCTC 3 cut(s) 236, 746, 901
BstMBI GATC 3 cut(s) 178, 777, 887
BstMCI CGRYCG 1 cut(s) 688
BstMWI GCNNNNNNNGC 1 cut(s) 298
BstNI CCWGG 1 cut(s) 260
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 1 cut(s) 258
BstSLI GKGCMC 1 cut(s) 906
BstUI CGCG 1 cut(s) 966
BstV1I GCAGC 2 cut(s) 223, 276
BstX2I RGATCY 1 cut(s) 777
BstYI RGATCY 1 cut(s) 777
BstZI CGGCCG 1 cut(s) 685
Bsu15I ATCGAT 3 cut(s) 177, 350, 533
Bsu36I CCTNAGG 1 cut(s) 339
BsuRI GGCC 8 cut(s) 87, 106, 198, 218, 490, 687, 773, 1026
BsuTUI ATCGAT 3 cut(s) 177, 350, 533
BtgI CCRYGG 3 cut(s) 101, 271, 790
BtsIMutI CAGTG 2 cut(s) 475, 484
Cac8I GCNNGC 2 cut(s) 380, 452
CciI TCATGA 1 cut(s) 316
Cfr13I GGNCC 6 cut(s) 19, 85, 105, 197, 771, 941
ClaI ATCGAT 3 cut(s) 177, 350, 533
CseI GACGC 1 cut(s) 670
Csp6I GTAC 3 cut(s) 355, 391, 847
CviAII CATG 8 cut(s) 272, 317, 451, 498, 637, 700, 791, 1009
CviQI GTAC 3 cut(s) 355, 391, 847
DdeI CTNAG 3 cut(s) 126, 339, 694
DpnI GATC 3 cut(s) 180, 779, 889
DpnII GATC 3 cut(s) 178, 777, 887
EaeI YGGCCR 3 cut(s) 216, 685, 1024
EagI CGGCCG 1 cut(s) 685
Eam1104I CTCTTC 2 cut(s) 483, 591
EarI CTCTTC 2 cut(s) 483, 591
EclXI CGGCCG 1 cut(s) 685
Eco130I CCWWGG 2 cut(s) 271, 790
Eco24I GRGCYC 1 cut(s) 665
Eco31I GGTCTC 1 cut(s) 746
Eco47I GGWCC 2 cut(s) 19, 941
Eco52I CGGCCG 1 cut(s) 685
Eco57I CTGAAG 1 cut(s) 380
Eco72I CACGTG 1 cut(s) 1064
Eco81I CCTNAGG 1 cut(s) 339
Eco88I CYCGRG 2 cut(s) 82, 665
EcoRII CCWGG 1 cut(s) 258
EcoT14I CCWWGG 2 cut(s) 271, 790
EcoT38I GRGCYC 1 cut(s) 665
ErhI CCWWGG 2 cut(s) 271, 790
FaeI CATG 8 cut(s) 275, 320, 454, 501, 640, 703, 794, 1012
FaqI GGGAC 1 cut(s) 927
FatI CATG 8 cut(s) 271, 316, 450, 497, 636, 699, 790, 1008
Fnu4HI GCNGC 2 cut(s) 237, 290
FriOI GRGCYC 1 cut(s) 665
Fsp4HI GCNGC 2 cut(s) 237, 290
FspBI CTAG 4 cut(s) 93, 191, 590, 755
GluI GCNGC 2 cut(s) 237, 290
GsaI CCCAGC 1 cut(s) 172
GsuI CTGGAG 1 cut(s) 314
HaeIII GGCC 8 cut(s) 87, 106, 198, 218, 490, 687, 773, 1026
HapII CCGG 1 cut(s) 950
HgaI GACGC 1 cut(s) 670
Hin1I GRCGYC 1 cut(s) 681
Hin1II CATG 8 cut(s) 275, 320, 454, 501, 640, 703, 794, 1012
HincII GTYRAC 3 cut(s) 559, 634, 711
HindII GTYRAC 3 cut(s) 559, 634, 711
HinfI GANTC 4 cut(s) 80, 132, 370, 894
HpaI GTTAAC 1 cut(s) 559
HpaII CCGG 1 cut(s) 950
HphI GGTGA 3 cut(s) 277, 556, 776
Hpy166II GTNNAC 4 cut(s) 268, 559, 634, 711
Hpy188I TCNGA 6 cut(s) 50, 127, 567, 660, 697, 887
Hpy188III TCNNGA 6 cut(s) 136, 317, 338, 891, 1001, 1052
Hpy8I GTNNAC 4 cut(s) 268, 559, 634, 711
Hpy99I CGWCG 5 cut(s) 313, 680, 686, 971, 1040
HpyAV CCTTC 6 cut(s) 47, 118, 355, 415, 430, 928
HpyCH4III ACNGT 4 cut(s) 470, 676, 715, 764
HpyCH4IV ACGT 3 cut(s) 155, 747, 1063
HpyCH4V TGCA 3 cut(s) 329, 501, 826
HpyF10VI GCNNNNNNNGC 1 cut(s) 298
HpyF3I CTNAG 3 cut(s) 126, 339, 694
HpySE526I ACGT 3 cut(s) 155, 747, 1063
Hsp92I GRCGYC 1 cut(s) 681
Hsp92II CATG 8 cut(s) 275, 320, 454, 501, 640, 703, 794, 1012
KspAI GTTAAC 1 cut(s) 559
Kzo9I GATC 3 cut(s) 178, 777, 887
LguI GCTCTTC 1 cut(s) 483
LmnI GCTCC 4 cut(s) 286, 340, 794, 859
Lsp1109I GCAGC 2 cut(s) 223, 276
MaeI CTAG 4 cut(s) 93, 191, 590, 755
MaeII ACGT 3 cut(s) 155, 747, 1063
MaeIII GTNAC 4 cut(s) 56, 583, 689, 980
MalI GATC 3 cut(s) 180, 779, 889
MboI GATC 3 cut(s) 178, 777, 887
MboII GAAGA 5 cut(s) 247, 470, 605, 608, 1012
MflI RGATCY 1 cut(s) 777
MhlI GDGCHC 2 cut(s) 665, 906
MlsI TGGCCA 1 cut(s) 218
MluCI AATT 3 cut(s) 10, 37, 526
MluNI TGGCCA 1 cut(s) 218
MlyI GAGTC 3 cut(s) 74, 379, 903
MmeI TCCRAC 2 cut(s) 210, 559
Mox20I TGGCCA 1 cut(s) 218
MroXI GAANNNNTTC 1 cut(s) 1016
MscI TGGCCA 1 cut(s) 218
MseI TTAA 3 cut(s) 387, 558, 750
MslI CAYNNNNRTG 2 cut(s) 566, 704
Msp20I TGGCCA 1 cut(s) 218
MspI CCGG 1 cut(s) 950
MspR9I CCNGG 1 cut(s) 260
MvaI CCWGG 1 cut(s) 260
MvnI CGCG 1 cut(s) 966
MwoI GCNNNNNNNGC 1 cut(s) 298
NcoI CCATGG 2 cut(s) 271, 790
NdeII GATC 3 cut(s) 178, 777, 887
NlaIII CATG 8 cut(s) 275, 320, 454, 501, 640, 703, 794, 1012
NlaIV GGNNCC 3 cut(s) 622, 918, 943
NmeAIII GCCGAG 2 cut(s) 265, 799
NmuCI GTSAC 2 cut(s) 583, 689
NspI RCATGY 1 cut(s) 454
NspV TTCGAA 1 cut(s) 871
PaeI GCATGC 1 cut(s) 454
PagI TCATGA 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 483
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 1065
PdmI GAANNNNTTC 1 cut(s) 1016
PfeI GAWTC 1 cut(s) 132
PkrI GCNGC 2 cut(s) 238, 291
PleI GAGTC 3 cut(s) 74, 378, 902
PmaCI CACGTG 1 cut(s) 1064
PmlI CACGTG 1 cut(s) 1064
PpsI GAGTC 3 cut(s) 74, 378, 902
Ppu21I YACGTR 2 cut(s) 156, 1064
Psp6I CCWGG 1 cut(s) 258
PspCI CACGTG 1 cut(s) 1064
PspFI CCCAGC 1 cut(s) 168
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 3 cut(s) 622, 918, 943
PspPI GGNCC 6 cut(s) 19, 85, 105, 197, 771, 941
PsuI RGATCY 1 cut(s) 777
RsaI GTAC 3 cut(s) 356, 392, 848
RsaNI GTAC 3 cut(s) 355, 391, 847
RseI CAYNNNNRTG 2 cut(s) 566, 704
SapI GCTCTTC 1 cut(s) 483
SaqAI TTAA 3 cut(s) 387, 558, 750
SatI GCNGC 2 cut(s) 237, 290
Sau3AI GATC 3 cut(s) 178, 777, 887
Sau96I GGNCC 6 cut(s) 19, 85, 105, 197, 771, 941
SchI GAGTC 3 cut(s) 74, 379, 903
ScrFI CCNGG 1 cut(s) 260
SduI GDGCHC 2 cut(s) 665, 906
SfuI TTCGAA 1 cut(s) 871
SinI GGWCC 2 cut(s) 19, 941
SmiMI CAYNNNNRTG 2 cut(s) 566, 704
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
SphI GCATGC 1 cut(s) 454
Sse9I AATT 3 cut(s) 10, 37, 526
SsiI CCGC 1 cut(s) 857
SspMI CTAG 4 cut(s) 93, 191, 590, 755
StyD4I CCNGG 1 cut(s) 258
StyI CCWWGG 2 cut(s) 271, 790
TaaI ACNGT 4 cut(s) 470, 676, 715, 764
TaiI ACGT 3 cut(s) 158, 750, 1066
TaqI TCGA 7 cut(s) 15, 177, 231, 350, 533, 871, 1053
TaqII GACCGA 1 cut(s) 929
TasI AATT 3 cut(s) 10, 37, 526
TatI WGTACW 1 cut(s) 846
TfiI GAWTC 1 cut(s) 132
Tru1I TTAA 3 cut(s) 387, 558, 750
Tru9I TTAA 3 cut(s) 387, 558, 750
TscAI CASTG 2 cut(s) 475, 491
TseFI GTSAC 2 cut(s) 583, 689
TseI GCWGC 2 cut(s) 236, 289
Tsp45I GTSAC 2 cut(s) 583, 689
TspDTI ATGAA 4 cut(s) 519, 620, 653, 1025
TspGWI ACGGA 2 cut(s) 408, 984
TspRI CASTG 2 cut(s) 475, 491
VpaK11BI GGWCC 2 cut(s) 19, 941
XapI RAATTY 1 cut(s) 526
XceI RCATGY 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 1016
XspI CTAG 4 cut(s) 93, 191, 590, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.