Rh5DG183100

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012769
Physical Location & Seq
Forward (+)
20294526 .. 20295533
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG183100.1

Sequence Viewer

Length: 714 bp
ATGGAACGAGTTTTGTGCCGGAGAAAGAGAAGGCTTTGGGGTGGAAGGACTATGTTAGCATGGTGTACTCCAGTGATGCTGATGCTCTCAGTCACTGGCCTAACGAGTGCAAGGAAGTTGCACTTGAGTACCTTAAAAGAATCAATGCATATGGTAAAGAAAATAGTGAAAACTTTGATTGAGAAGCTCGGAGTGACACTTGAGGACTCAAAACTAGATGGACTCGTTGGGTTAAAGATGGTGAACATGGTATATCCAAAAAAAATAAAGATGGTGAGCATGAACTTTTACCCAACATGCCCTAATCCGGAGCTCACTGTCGGAGTTGGGCGTCACTCGGACATGGGCACCTTAACCGTTTTACTGCAAGATGGAATTGGTGGTTTGTATGTGAAGGTTGAAGAAGACATGAATGCTGGAAAGAAAGGAGAGTGGATCGAGATCCCTCCTATCCCTGGAGCCTTGATACTTGGTAATGGGAGATATAAAAGTGCCGAATACAGAGTTCGAACTACAAACGCTCAATCAAGAGTGTCTGTTCCGATATTTACCATTCCAAATCCAACAGAAATAATTGGGCCGCTGCCGGAGGTGGTGGCGCGTGATGGAGTGGCTCGTTACAGTGACATGGTTTTTGGAGATTATATGAACAACTTTTTCGGCAATGCACATGAGGGCAAGAAGTCTCATGATTATGCCCAGATTAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

237

Amino Acids

26.46

Weight (kDa)

9.53

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 91 - 184 1.1e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12900
fragaria_vesca FvH4_3g15580
malus_domestica MD00G1188900.v1.1 MD05G1221300.v1.1
prunus_persica Prupe.4G138300_v2.0.a1
pyrus_communis pycom05g20470
rosa_chinensis RchiOBHm_Chr5g0026211
rosa_laevigata RLG00000032912
rosa_multiflora Rmu_co8420971.1_g000001 Rmu_sc0002909.1_g000002 Rmu_sc0005061.1_g000001
rosa_roxburghii Rroxscaffold_1G00053100
rosa_rugosa Rorug05G0091600
rosa_samantha Rh5AG184500 Rh5BG181400 Rh5CG200500 Rh5DG183100
rosa_wichuraiana Rw5G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 347
AccII CGCG 1 cut(s) 601
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 1 cut(s) 581
AclWI GGATC 2 cut(s) 436, 443
AcyI GRCGYC 1 cut(s) 331
AfaI GTAC 2 cut(s) 67, 130
AfiI CCNNNNNNNGG 2 cut(s) 307, 455
AgsI TTSAA 1 cut(s) 401
AjnI CCWGG 1 cut(s) 454
AluBI AGCT 2 cut(s) 187, 313
AluI AGCT 2 cut(s) 187, 313
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 690
AlwI GGATC 2 cut(s) 436, 443
AlwNI CAGNNNCTG 1 cut(s) 95
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 2 cut(s) 97, 578
ApeKI GCWGC 1 cut(s) 583
ArsI GACNNNNNNTTYG 2 cut(s) 617, 649
AseI ATTAAT 1 cut(s) 705
AspLEI GCGC 1 cut(s) 601
AspS9I GGNCC 1 cut(s) 578
AsuHPI GGTGA 2 cut(s) 253, 286
AsuII TTCGAA 1 cut(s) 508
BaeGI GKGCMC 1 cut(s) 350
BaeI ACNNNNGTAYC 2 cut(s) 112, 145
BanI GGYRCC 1 cut(s) 347
BanII GRGCYC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 570
BccI CCATC 5 cut(s) 212, 232, 265, 365, 599
BcgI CGANNNNNNTGC 1 cut(s) 31
BciT130I CCWGG 1 cut(s) 456
BcoDI GTCTC 1 cut(s) 690
BfaI CTAG 1 cut(s) 215
BisI GCNGC 2 cut(s) 581, 584
BlsI GCNGC 2 cut(s) 582, 585
Bme1390I CCNGG 1 cut(s) 456
BmgT120I GGNCC 1 cut(s) 578
BmiI GGNNCC 2 cut(s) 349, 460
BmrFI CCNGG 1 cut(s) 456
BmsI GCATC 2 cut(s) 66, 72
BpiI GAAGAC 1 cut(s) 411
BpmI CTGGAG 2 cut(s) 54, 477
Bpu14I TTCGAA 1 cut(s) 508
BpuEI CTTGAG 2 cut(s) 145, 221
BsaBI GATNNNNATC 1 cut(s) 440
BsaHI GRCGYC 1 cut(s) 331
BsaJI CCNNGG 1 cut(s) 454
BsaWI WCCGGW 1 cut(s) 307
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 455
Bse1I ACTGG 2 cut(s) 71, 100
Bse3DI GCAATG 1 cut(s) 670
Bse8I GATNNNNATC 1 cut(s) 440
BseAI TCCGGA 1 cut(s) 307
BseBI CCWGG 1 cut(s) 456
BseDI CCNNGG 1 cut(s) 454
BseJI GATNNNNATC 1 cut(s) 440
BseLI CCNNNNNNNGG 2 cut(s) 307, 455
BseMI GCAATG 1 cut(s) 670
BseMII CTCAG 1 cut(s) 102
BseNI ACTGG 2 cut(s) 71, 100
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 601
BshFI GGCC 2 cut(s) 99, 580
BshNI GGYRCC 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 3 cut(s) 19, 308, 587
BslI CCNNNNNNNGG 2 cut(s) 307, 455
BsmAI GTCTC 1 cut(s) 690
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 2 cut(s) 99, 580
Bsp119I TTCGAA 1 cut(s) 508
Bsp1286I GDGCHC 2 cut(s) 315, 350
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 2 cut(s) 435, 441
BspACI CCGC 1 cut(s) 581
BspANI GGCC 2 cut(s) 99, 580
BspCNI CTCAG 1 cut(s) 101
BspEI TCCGGA 1 cut(s) 307
BspFNI CGCG 1 cut(s) 601
BspHI TCATGA 1 cut(s) 688
BspLI GGNNCC 2 cut(s) 349, 460
BspPI GGATC 2 cut(s) 436, 443
BspT104I TTCGAA 1 cut(s) 508
BspT107I GGYRCC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 670
BsrI ACTGG 2 cut(s) 71, 100
BssECI CCNNGG 1 cut(s) 454
BssMI GATC 2 cut(s) 435, 441
BssNI GRCGYC 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 456
Bst4CI ACNGT 3 cut(s) 319, 358, 623
BstACI GRCGYC 1 cut(s) 331
BstBI TTCGAA 1 cut(s) 508
BstDEI CTNAG 1 cut(s) 88
BstFNI CGCG 1 cut(s) 601
BstHHI GCGC 1 cut(s) 601
BstKTI GATC 2 cut(s) 438, 444
BstMAI GTCTC 1 cut(s) 690
BstMBI GATC 2 cut(s) 435, 441
BstNI CCWGG 1 cut(s) 456
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 454
BstSLI GKGCMC 1 cut(s) 350
BstUI CGCG 1 cut(s) 601
BstV1I GCAGC 1 cut(s) 570
BstV2I GAAGAC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 441
BstYI RGATCY 1 cut(s) 441
BsuRI GGCC 2 cut(s) 99, 580
BtsIMutI CAGTG 4 cut(s) 78, 93, 315, 628
CaiI CAGNNNCTG 1 cut(s) 95
CciI TCATGA 1 cut(s) 688
CfoI GCGC 1 cut(s) 601
Cfr13I GGNCC 1 cut(s) 578
CseI GACGC 1 cut(s) 320
Csp6I GTAC 2 cut(s) 66, 129
CviAII CATG 9 cut(s) 60, 247, 280, 297, 343, 409, 628, 671, 689
CviJI RGCY 7 cut(s) 34, 99, 187, 313, 461, 580, 614
CviKI_1 RGCY 7 cut(s) 34, 99, 187, 313, 461, 580, 614
CviQI GTAC 2 cut(s) 66, 129
DdeI CTNAG 1 cut(s) 88
DpnI GATC 2 cut(s) 437, 443
DpnII GATC 2 cut(s) 435, 441
Ecl136II GAGCTC 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 315
Eco53kI GAGCTC 1 cut(s) 313
EcoICRI GAGCTC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 454
EcoT22I ATGCAT 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 315
FaeI CATG 9 cut(s) 63, 250, 283, 300, 346, 412, 631, 674, 692
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 9 cut(s) 59, 246, 279, 296, 342, 408, 627, 670, 688
FauNDI CATATG 1 cut(s) 150
Fnu4HI GCNGC 2 cut(s) 581, 584
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 2 cut(s) 581, 584
FspBI CTAG 1 cut(s) 215
GlaI GCGC 1 cut(s) 600
GluI GCNGC 2 cut(s) 581, 584
GsuI CTGGAG 2 cut(s) 54, 477
HaeIII GGCC 2 cut(s) 99, 580
HapII CCGG 3 cut(s) 19, 308, 587
HgaI GACGC 1 cut(s) 320
HhaI GCGC 1 cut(s) 601
Hin1I GRCGYC 1 cut(s) 331
Hin1II CATG 9 cut(s) 63, 250, 283, 300, 346, 412, 631, 674, 692
Hin6I GCGC 1 cut(s) 599
HinP1I GCGC 1 cut(s) 599
HinfI GANTC 3 cut(s) 140, 206, 222
HpaII CCGG 3 cut(s) 19, 308, 587
HphI GGTGA 2 cut(s) 253, 286
Hpy166II GTNNAC 2 cut(s) 66, 244
Hpy188I TCNGA 4 cut(s) 191, 323, 340, 543
Hpy188III TCNNGA 4 cut(s) 308, 439, 528, 689
Hpy8I GTNNAC 2 cut(s) 66, 244
HpyAV CCTTC 3 cut(s) 24, 39, 388
HpyCH4III ACNGT 3 cut(s) 319, 358, 623
HpyCH4V TGCA 5 cut(s) 110, 121, 148, 367, 668
HpyF3I CTNAG 1 cut(s) 88
Hsp92I GRCGYC 1 cut(s) 331
Hsp92II CATG 9 cut(s) 63, 250, 283, 300, 346, 412, 631, 674, 692
HspAI GCGC 1 cut(s) 599
Kpn2I TCCGGA 1 cut(s) 307
Kzo9I GATC 2 cut(s) 435, 441
LmnI GCTCC 2 cut(s) 310, 458
LpnPI CCDG 8 cut(s) 32, 81, 84, 321, 402, 441, 468, 600
Lsp1109I GCAGC 1 cut(s) 570
LweI GCATC 2 cut(s) 66, 72
MaeI CTAG 1 cut(s) 215
MaeIII GTNAC 5 cut(s) 91, 193, 332, 617, 623
MalI GATC 2 cut(s) 437, 443
MboI GATC 2 cut(s) 435, 441
MboII GAAGA 2 cut(s) 413, 416
MflI RGATCY 1 cut(s) 441
MhlI GDGCHC 2 cut(s) 315, 350
MluCI AATT 3 cut(s) 375, 573, 709
MlyI GAGTC 2 cut(s) 200, 216
MmeI TCCRAC 2 cut(s) 301, 587
MnlI CCTC 4 cut(s) 196, 456, 583, 667
Mph1103I ATGCAT 1 cut(s) 150
MroI TCCGGA 1 cut(s) 307
MseI TTAA 4 cut(s) 134, 233, 353, 705
MslI CAYNNNNRTG 1 cut(s) 693
MspA1I CMGCKG 1 cut(s) 583
MspI CCGG 3 cut(s) 19, 308, 587
MspR9I CCNGG 1 cut(s) 456
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 1 cut(s) 456
MvnI CGCG 1 cut(s) 601
NdeI CATATG 1 cut(s) 150
NdeII GATC 2 cut(s) 435, 441
NlaIII CATG 9 cut(s) 63, 250, 283, 300, 346, 412, 631, 674, 692
NlaIV GGNNCC 2 cut(s) 349, 460
NmuCI GTSAC 4 cut(s) 91, 193, 332, 623
NsiI ATGCAT 1 cut(s) 150
NspI RCATGY 1 cut(s) 300
NspV TTCGAA 1 cut(s) 508
PagI TCATGA 1 cut(s) 688
PctI GAATGC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 582, 585
PleI GAGTC 2 cut(s) 200, 216
PpsI GAGTC 2 cut(s) 200, 216
PshBI ATTAAT 1 cut(s) 705
Psp124BI GAGCTC 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 454
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 2 cut(s) 349, 460
PspPI GGNCC 1 cut(s) 578
PstNI CAGNNNCTG 1 cut(s) 95
PsuI RGATCY 1 cut(s) 441
RsaI GTAC 2 cut(s) 67, 130
RsaNI GTAC 2 cut(s) 66, 129
RseI CAYNNNNRTG 1 cut(s) 693
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 4 cut(s) 134, 233, 353, 705
SatI GCNGC 2 cut(s) 581, 584
Sau3AI GATC 2 cut(s) 435, 441
Sau96I GGNCC 1 cut(s) 578
SchI GAGTC 2 cut(s) 200, 216
ScrFI CCNGG 1 cut(s) 456
SduI GDGCHC 2 cut(s) 315, 350
SetI ASST 6 cut(s) 134, 189, 315, 353, 399, 594
SfaNI GCATC 2 cut(s) 66, 72
SfuI TTCGAA 1 cut(s) 508
SmiMI CAYNNNNRTG 1 cut(s) 693
SmlI CTYRAG 2 cut(s) 124, 200
SmoI CTYRAG 2 cut(s) 124, 200
Sse9I AATT 3 cut(s) 375, 573, 709
SsiI CCGC 1 cut(s) 581
SspMI CTAG 1 cut(s) 215
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 454
TaaI ACNGT 3 cut(s) 319, 358, 623
TaqI TCGA 2 cut(s) 438, 508
TasI AATT 3 cut(s) 375, 573, 709
TatI WGTACW 1 cut(s) 65
TauI GCSGC 1 cut(s) 583
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 4 cut(s) 134, 233, 353, 705
Tru9I TTAA 4 cut(s) 134, 233, 353, 705
TscAI CASTG 4 cut(s) 78, 100, 322, 628
TseFI GTSAC 4 cut(s) 91, 193, 332, 623
TseI GCWGC 1 cut(s) 583
Tsp45I GTSAC 4 cut(s) 91, 193, 332, 623
TspDTI ATGAA 3 cut(s) 296, 425, 662
TspRI CASTG 4 cut(s) 78, 100, 322, 628
VspI ATTAAT 1 cut(s) 705
XceI RCATGY 1 cut(s) 300
XspI CTAG 1 cut(s) 215
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.