Rw5G016730

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012769
Physical Location & Seq
Forward (+)
21296387 .. 21298074
1688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016730.1

Sequence Viewer

Length: 1020 bp
ATGGCTCCAAGTTTCAATGATGAAAACTCACTATTCAACTTTGTTGTCAGGGATGGCAATGGTGTGAAGGGCATGGTGGACTTAGGCCTATCCAAAGTACCTGAACAATACATACAACCACCTCATGAGCGTATACTGAAGCTTAATGCAAGCTCACTAAATGAGCTCAGACCAATCGATCTGTCTAAGCTTGATGCGGTTGATCATGAACAAGTGGCTAATGAGATCGTTAATGCTGCCGAGGTTCTTGGGTTCTTCCAATTGCTCGAGTCACTGAAAGATGCTGCAAACAATTTCTTCAAGCAAGCGTCGAAGAAGAAAGCTGCTTACCGAAAAGGTGTGAGCCCGAGCCGATACGTGAAGTATGGAACGATTTTTGTGCCGGAGAAAGAGAAGGCTTTGGAGTGGAAGGACTATGTTAGCATGGTGTACTCTAGTGATGCTGATGCTCTCAGGCCTAACGAGTGCAAGGAAGTTGCACTTGAGTACCTTAAAACATCAATGCATATGGTAAAGCAAATAGTGAAAACTTTGATTGAGAAGCTCGGAGTGACACTTGAGGACTCAAAACTAGATGGACTCGTTGGGTTAAAGATGCTTACTGTCGGAGTTGGGCGTCACTCGGACATGGGCACCTTAACCGTTTTACTGCAAGATGGAATTGGTGGTTTGTATGTGAAGGTTGAAGAAGACATGAATGCAGGAAAGAAAGGAGAGTGGATCGAGATCCGTCCTATCCCTGGGGCTTTGGTCATCAACATTGGTGATACATTACAGATACTTAGTAATGGGAGATATAAAAGTGCCGAACACAGAGTTCGAACTACAAACGCTCAAGTGTCTGTTTCGATATTTACCATTCCAAATCCAACAGAAATAATTGGGCCGCTGCCGGAGGTGGTGGCGCCTGATGGAGAGGCTCGTTACCGTGACATGGTTTTTGGAGATTATATGAACAACTTTTTCGGCAATGCACATGAGGGCAAGAAGTCTCTGGATTATGCCCAGATTAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

339

Amino Acids

37.62

Weight (kDa)

5.8

Isoelectric Point (pI)

28.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 59 - 149 1.3e-08 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 200 - 285 1.5e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12900
fragaria_vesca FvH4_3g15580
malus_domestica MD00G1188900.v1.1 MD05G1221300.v1.1
prunus_persica Prupe.4G138300_v2.0.a1
pyrus_communis pycom05g20470
rosa_chinensis RchiOBHm_Chr5g0026211
rosa_laevigata RLG00000032912
rosa_multiflora Rmu_co8420971.1_g000001 Rmu_sc0002909.1_g000002 Rmu_sc0005061.1_g000001
rosa_roxburghii Rroxscaffold_1G00053100
rosa_rugosa Rorug05G0091600
rosa_samantha Rh5AG184500 Rh5BG181400 Rh5CG200500 Rh5DG183100
rosa_wichuraiana Rw5G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 632, 904
AccI GTMKAC 1 cut(s) 133
AciI CCGC 2 cut(s) 197, 887
AclWI GGATC 2 cut(s) 721, 728
AcuI CTGAAG 1 cut(s) 158
AcyI GRCGYC 2 cut(s) 616, 905
AfaI GTAC 3 cut(s) 99, 431, 488
AfiI CCNNNNNNNGG 2 cut(s) 740, 934
AgsI TTSAA 4 cut(s) 16, 37, 301, 686
AjnI CCWGG 1 cut(s) 739
AluBI AGCT 6 cut(s) 142, 153, 166, 190, 323, 544
AluI AGCT 6 cut(s) 142, 153, 166, 190, 323, 544
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 1 cut(s) 996
AlwI GGATC 2 cut(s) 721, 728
Ama87I CYCGRG 2 cut(s) 266, 346
AoxI GGCC 3 cut(s) 85, 455, 884
ApeKI GCWGC 4 cut(s) 236, 284, 323, 889
ArsI GACNNNNNNTTYG 2 cut(s) 923, 955
AseI ATTAAT 1 cut(s) 1011
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 1 cut(s) 884
AsuHPI GGTGA 1 cut(s) 776
AsuII TTCGAA 1 cut(s) 820
AvaI CYCGRG 2 cut(s) 266, 346
BaeGI GKGCMC 1 cut(s) 635
BaeI ACNNNNGTAYC 2 cut(s) 470, 503
BanI GGYRCC 2 cut(s) 632, 904
BanII GRGCYC 2 cut(s) 168, 347
BbsI GAAGAC 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 4 cut(s) 223, 271, 310, 876
BccI CCATC 4 cut(s) 47, 569, 650, 905
BcgI CGANNNNNNTGC 2 cut(s) 361, 395
BciT130I CCWGG 1 cut(s) 741
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 996
BfaI CTAG 2 cut(s) 435, 572
BfoI RGCGCY 1 cut(s) 908
BisI GCNGC 5 cut(s) 237, 285, 324, 887, 890
BlsI GCNGC 5 cut(s) 238, 286, 325, 888, 891
Bme1390I CCNGG 1 cut(s) 741
BmeT110I CYCGRG 2 cut(s) 266, 346
BmgT120I GGNCC 1 cut(s) 884
BmiI GGNNCC 3 cut(s) 6, 634, 906
BmrFI CCNGG 1 cut(s) 741
BmsI GCATC 5 cut(s) 184, 271, 430, 436, 585
BpiI GAAGAC 1 cut(s) 696
Bpu14I TTCGAA 1 cut(s) 820
BpuEI CTTGAG 3 cut(s) 503, 578, 819
Bsa29I ATCGAT 1 cut(s) 177
BsaAI YACGTR 1 cut(s) 358
BsaBI GATNNNNATC 1 cut(s) 725
BsaHI GRCGYC 2 cut(s) 616, 905
BsaJI CCNNGG 3 cut(s) 240, 739, 740
Bsc4I CCNNNNNNNGG 2 cut(s) 740, 934
Bse3DI GCAATG 2 cut(s) 64, 976
Bse8I GATNNNNATC 1 cut(s) 725
BseBI CCWGG 1 cut(s) 741
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 240, 739, 740
BseGI GGATG 1 cut(s) 58
BseJI GATNNNNATC 1 cut(s) 725
BseLI CCNNNNNNNGG 2 cut(s) 740, 934
BseMI GCAATG 2 cut(s) 64, 976
BseMII CTCAG 2 cut(s) 181, 466
BseSI GKGCMC 1 cut(s) 635
BseXI GCAGC 4 cut(s) 223, 271, 310, 876
BshFI GGCC 3 cut(s) 87, 457, 886
BshNI GGYRCC 2 cut(s) 632, 904
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 2 cut(s) 266, 346
BsiSI CCGG 2 cut(s) 383, 893
BslI CCNNNNNNNGG 2 cut(s) 740, 934
BsmAI GTCTC 1 cut(s) 996
BsmI GAATGC 1 cut(s) 703
BsnI GGCC 3 cut(s) 87, 457, 886
BsoBI CYCGRG 2 cut(s) 266, 346
Bsp119I TTCGAA 1 cut(s) 820
Bsp1286I GDGCHC 3 cut(s) 168, 347, 635
Bsp143I GATC 5 cut(s) 178, 202, 225, 720, 726
BspACI CCGC 2 cut(s) 197, 887
BspANI GGCC 3 cut(s) 87, 457, 886
BspCNI CTCAG 2 cut(s) 180, 465
BspDI ATCGAT 1 cut(s) 177
BspHI TCATGA 2 cut(s) 124, 205
BspLI GGNNCC 3 cut(s) 6, 634, 906
BspPI GGATC 2 cut(s) 721, 728
BspT104I TTCGAA 1 cut(s) 820
BspT107I GGYRCC 2 cut(s) 632, 904
BsrDI GCAATG 2 cut(s) 64, 976
BssECI CCNNGG 3 cut(s) 240, 739, 740
BssMI GATC 5 cut(s) 178, 202, 225, 720, 726
BssNAI GTATAC 1 cut(s) 134
BssNI GRCGYC 2 cut(s) 616, 905
Bst1107I GTATAC 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 3 cut(s) 604, 643, 929
BstACI GRCGYC 2 cut(s) 616, 905
BstBAI YACGTR 1 cut(s) 358
BstBI TTCGAA 1 cut(s) 820
BstC8I GCNNGC 2 cut(s) 151, 306
BstDEI CTNAG 5 cut(s) 82, 167, 186, 452, 782
BstF5I GGATG 1 cut(s) 58
BstH2I RGCGCY 1 cut(s) 908
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 5 cut(s) 181, 205, 228, 723, 729
BstMAI GTCTC 1 cut(s) 996
BstMBI GATC 5 cut(s) 178, 202, 225, 720, 726
BstNI CCWGG 1 cut(s) 741
BstSCI CCNGG 1 cut(s) 739
BstSLI GKGCMC 1 cut(s) 635
BstV1I GCAGC 4 cut(s) 223, 271, 310, 876
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 1 cut(s) 726
BstYI RGATCY 1 cut(s) 726
BstZ17I GTATAC 1 cut(s) 134
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 3 cut(s) 87, 457, 886
BsuTUI ATCGAT 1 cut(s) 177
BtsCI GGATG 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 272
Cac8I GCNNGC 2 cut(s) 151, 306
CciI TCATGA 2 cut(s) 124, 205
CfoI GCGC 1 cut(s) 907
Cfr13I GGNCC 1 cut(s) 884
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 2 cut(s) 297, 605
Csp6I GTAC 3 cut(s) 98, 430, 487
CviAII CATG 8 cut(s) 73, 125, 206, 424, 628, 694, 934, 977
CviQI GTAC 3 cut(s) 98, 430, 487
DdeI CTNAG 5 cut(s) 82, 167, 186, 452, 782
DinI GGCGCC 1 cut(s) 906
DpnI GATC 5 cut(s) 180, 204, 227, 722, 728
DpnII GATC 5 cut(s) 178, 202, 225, 720, 726
Ecl136II GAGCTC 1 cut(s) 166
Eco147I AGGCCT 2 cut(s) 87, 457
Eco24I GRGCYC 2 cut(s) 168, 347
Eco53kI GAGCTC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 158
Eco88I CYCGRG 2 cut(s) 266, 346
EcoICRI GAGCTC 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 739
EcoT22I ATGCAT 1 cut(s) 507
EcoT38I GRGCYC 2 cut(s) 168, 347
EgeI GGCGCC 1 cut(s) 906
EheI GGCGCC 1 cut(s) 906
FaeI CATG 8 cut(s) 76, 128, 209, 427, 631, 697, 937, 980
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FatI CATG 8 cut(s) 72, 124, 205, 423, 627, 693, 933, 976
FauNDI CATATG 1 cut(s) 507
FbaI TGATCA 1 cut(s) 202
FblI GTMKAC 1 cut(s) 133
Fnu4HI GCNGC 5 cut(s) 237, 285, 324, 887, 890
FokI GGATG 1 cut(s) 65
FriOI GRGCYC 2 cut(s) 168, 347
Fsp4HI GCNGC 5 cut(s) 237, 285, 324, 887, 890
FspBI CTAG 2 cut(s) 435, 572
GlaI GCGC 1 cut(s) 906
GluI GCNGC 5 cut(s) 237, 285, 324, 887, 890
HaeII RGCGCY 1 cut(s) 908
HaeIII GGCC 3 cut(s) 87, 457, 886
HapII CCGG 2 cut(s) 383, 893
HgaI GACGC 2 cut(s) 297, 605
HhaI GCGC 1 cut(s) 907
Hin1I GRCGYC 2 cut(s) 616, 905
Hin1II CATG 8 cut(s) 76, 128, 209, 427, 631, 697, 937, 980
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HindIII AAGCTT 2 cut(s) 140, 188
HinfI GANTC 3 cut(s) 269, 563, 579
HpaII CCGG 2 cut(s) 383, 893
HphI GGTGA 1 cut(s) 776
Hpy166II GTNNAC 3 cut(s) 79, 134, 430
Hpy188I TCNGA 4 cut(s) 170, 548, 608, 625
Hpy188III TCNNGA 4 cut(s) 125, 206, 724, 995
Hpy8I GTNNAC 3 cut(s) 79, 134, 430
Hpy99I CGWCG 1 cut(s) 313
HpyAV CCTTC 4 cut(s) 61, 388, 403, 673
HpyCH4III ACNGT 3 cut(s) 604, 643, 929
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 8 cut(s) 149, 287, 468, 479, 505, 652, 701, 974
HpyF3I CTNAG 5 cut(s) 82, 167, 186, 452, 782
HpySE526I ACGT 1 cut(s) 357
Hsp92I GRCGYC 2 cut(s) 616, 905
Hsp92II CATG 8 cut(s) 76, 128, 209, 427, 631, 697, 937, 980
HspAI GCGC 1 cut(s) 905
KasI GGCGCC 1 cut(s) 904
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 5 cut(s) 178, 202, 225, 720, 726
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 4 cut(s) 223, 271, 310, 876
LweI GCATC 5 cut(s) 184, 271, 430, 436, 585
MaeI CTAG 2 cut(s) 435, 572
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 5 cut(s) 270, 550, 617, 923, 929
MalI GATC 5 cut(s) 180, 204, 227, 722, 728
MboI GATC 5 cut(s) 178, 202, 225, 720, 726
MboII GAAGA 6 cut(s) 247, 289, 325, 328, 698, 701
MfeI CAATTG 1 cut(s) 260
MflI RGATCY 1 cut(s) 726
MhlI GDGCHC 3 cut(s) 168, 347, 635
MluCI AATT 5 cut(s) 260, 292, 660, 879, 1015
Mly113I GGCGCC 1 cut(s) 905
MlyI GAGTC 3 cut(s) 278, 557, 573
MmeI TCCRAC 2 cut(s) 586, 893
MnlI CCTC 6 cut(s) 132, 235, 553, 889, 910, 973
Mph1103I ATGCAT 1 cut(s) 507
MseI TTAA 6 cut(s) 144, 231, 492, 590, 638, 1011
MspA1I CMGCKG 1 cut(s) 889
MspI CCGG 2 cut(s) 383, 893
MspR9I CCNGG 1 cut(s) 741
MunI CAATTG 1 cut(s) 260
Mva1269I GAATGC 1 cut(s) 703
MvaI CCWGG 1 cut(s) 741
NarI GGCGCC 1 cut(s) 905
NdeI CATATG 1 cut(s) 507
NdeII GATC 5 cut(s) 178, 202, 225, 720, 726
NlaIII CATG 8 cut(s) 76, 128, 209, 427, 631, 697, 937, 980
NlaIV GGNNCC 3 cut(s) 6, 634, 906
NmeAIII GCCGAG 1 cut(s) 265
NmuCI GTSAC 4 cut(s) 270, 550, 617, 929
NsiI ATGCAT 1 cut(s) 507
NspV TTCGAA 1 cut(s) 820
PaeR7I CTCGAG 1 cut(s) 266
PagI TCATGA 2 cut(s) 124, 205
PasI CCCWGGG 1 cut(s) 740
PceI AGGCCT 2 cut(s) 87, 457
PctI GAATGC 1 cut(s) 703
PkrI GCNGC 5 cut(s) 238, 286, 325, 888, 891
PleI GAGTC 3 cut(s) 277, 557, 573
PluTI GGCGCC 1 cut(s) 908
PpsI GAGTC 3 cut(s) 277, 557, 573
Ppu21I YACGTR 1 cut(s) 358
PshBI ATTAAT 1 cut(s) 1011
Psp124BI GAGCTC 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 739
PspGI CCWGG 1 cut(s) 739
PspN4I GGNNCC 3 cut(s) 6, 634, 906
PspPI GGNCC 1 cut(s) 884
PspXI VCTCGAGB 1 cut(s) 266
PsrI GAACNNNNNNTAC 2 cut(s) 96, 128
PsuI RGATCY 1 cut(s) 726
RsaI GTAC 3 cut(s) 99, 431, 488
RsaNI GTAC 3 cut(s) 98, 430, 487
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 6 cut(s) 144, 231, 492, 590, 638, 1011
SatI GCNGC 5 cut(s) 237, 285, 324, 887, 890
Sau3AI GATC 5 cut(s) 178, 202, 225, 720, 726
Sau96I GGNCC 1 cut(s) 884
SchI GAGTC 3 cut(s) 278, 557, 573
ScrFI CCNGG 1 cut(s) 741
SduI GDGCHC 3 cut(s) 168, 347, 635
SfaNI GCATC 5 cut(s) 184, 271, 430, 436, 585
SfoI GGCGCC 1 cut(s) 906
Sfr274I CTCGAG 1 cut(s) 266
SfuI TTCGAA 1 cut(s) 820
SlaI CTCGAG 1 cut(s) 266
SmlI CTYRAG 4 cut(s) 266, 482, 557, 834
SmoI CTYRAG 4 cut(s) 266, 482, 557, 834
Sse9I AATT 5 cut(s) 260, 292, 660, 879, 1015
SseBI AGGCCT 2 cut(s) 87, 457
SsiI CCGC 2 cut(s) 197, 887
SspDI GGCGCC 1 cut(s) 904
SspMI CTAG 2 cut(s) 435, 572
SstI GAGCTC 1 cut(s) 168
StuI AGGCCT 2 cut(s) 87, 457
StyD4I CCNGG 1 cut(s) 739
TaaI ACNGT 3 cut(s) 604, 643, 929
TaiI ACGT 1 cut(s) 360
TaqI TCGA 6 cut(s) 177, 267, 311, 723, 820, 848
TasI AATT 5 cut(s) 260, 292, 660, 879, 1015
TatI WGTACW 1 cut(s) 429
TauI GCSGC 1 cut(s) 889
Tru1I TTAA 6 cut(s) 144, 231, 492, 590, 638, 1011
Tru9I TTAA 6 cut(s) 144, 231, 492, 590, 638, 1011
TscAI CASTG 1 cut(s) 279
TseFI GTSAC 4 cut(s) 270, 550, 617, 929
TseI GCWGC 4 cut(s) 236, 284, 323, 889
Tsp45I GTSAC 4 cut(s) 270, 550, 617, 929
TspDTI ATGAA 4 cut(s) 36, 222, 710, 968
TspGWI ACGGA 1 cut(s) 719
TspRI CASTG 1 cut(s) 279
VspI ATTAAT 1 cut(s) 1011
XhoI CTCGAG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 133
XspI CTAG 2 cut(s) 435, 572
Zsp2I ATGCAT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.