Rroxscaffold_1G00053100

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012769
Physical Location & Seq
Reverse (-)
73941266 .. 73942812
1547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00053100.1

Sequence Viewer

Length: 1098 bp
ATGGCTCCAAGTTTCAATGATGAAAACTCACTATTCAACTTTGTTGTCAGGGATGGCAATGGCGTGAAGGGCATGGTGGACTTAGGCCTATCCAAAGTACCTGAACAATACATACAACCACCTCATGAGCGTATACTGAAGCTTAATGCAAGCTCACTAAATGAGCTCAGACCAATCGATCTGTCTAAGCTTGATGCGGTTGATCATGAACAAGTGGCTAATGAGATCGTTAATGCTGCCGAGGTTCTTGGGTTCTTCCAAGTTGTGAACCATGGCCTGCCTGTGGAGTTGCTGGAGTCTCTGAAAGATGCTGCAAACAAGTTCTTCGAGCAAGCGCCGGAGAAGAAAGCTGCTTACCGGAAAGGTGTGAGTCCGAGCCCGTACGTGAAGTATGGAACGAGTTTTGTGCCGGAGAAAGAGAAGGCTTTGGAGTGGAAGGACTATGTTAGCATGGTGTACTCCAGTGATGCTGATGCTCTCAGTCACTGGCCTAACGAGTGCAAGGAAGTTGCACTTGAATACCTTAAAACATCAATGCATATGGTAAAGAAAATAGTGAAAACTTTGATTGAGAAGCTCGGAGTGACACTTGAGGACTCAAAACTAGATGGACTCCTTGGGTTAAAGATGGTGAACATGAACTTTTACCCAACATGCCCTAATCCGGAGCTCACTGTCGGAGTTGGGCGCCACTCGGACATGGGCACCTTAACCGTTTTACTGCAAGATGGAATTGGTGGTTTGTATGTGAAGGTTGAAGAAGACATGAATGCTGGAAAGAAAGGAGAGTGGATCGATATCCCTCCTATCCCTGGAGCTTTGGTCATCAACATTGGTGATACATTACAGATACTTAGTAATGGAAGATATAAAAGTGCCGAACACAGAGTTCGAACTACAAACGCTCAATCAAGAGTGTCTGTTCCGATATTTACCATTCCAAATCCAACAGAAATAATTGGGCCGCTGCCGGAGGTGGTGGCCCGTGATGGAGTGGCTCGTTACCGTGACATGGTTTTTGGAGATTATATGAACAACTTTTTCGGCAATGCACATGAGGGCAAGAAGTCTCTTGATTATGCCCAGATTAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

365

Amino Acids

40.5

Weight (kDa)

5.49

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 59 - 165 2.7e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 210 - 312 1.4e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12900
fragaria_vesca FvH4_3g15580
malus_domestica MD00G1188900.v1.1 MD05G1221300.v1.1
prunus_persica Prupe.4G138300_v2.0.a1
pyrus_communis pycom05g20470
rosa_chinensis RchiOBHm_Chr5g0026211
rosa_laevigata RLG00000032912
rosa_multiflora Rmu_co8420971.1_g000001 Rmu_sc0002909.1_g000002 Rmu_sc0005061.1_g000001
rosa_roxburghii Rroxscaffold_1G00053100
rosa_rugosa Rorug05G0091600
rosa_samantha Rh5AG184500 Rh5BG181400 Rh5CG200500 Rh5DG183100
rosa_wichuraiana Rw5G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 687, 704
AccI GTMKAC 1 cut(s) 133
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 2 cut(s) 197, 965
AclWI GGATC 1 cut(s) 800
AcuI CTGAAG 1 cut(s) 158
AcyI GRCGYC 1 cut(s) 688
AfaI GTAC 3 cut(s) 99, 383, 458
AfiI CCNNNNNNNGG 4 cut(s) 283, 664, 812, 1012
AgsI TTSAA 4 cut(s) 16, 37, 518, 758
AjnI CCWGG 1 cut(s) 811
AluBI AGCT 8 cut(s) 142, 153, 166, 190, 350, 577, 670, 818
AluI AGCT 8 cut(s) 142, 153, 166, 190, 350, 577, 670, 818
Alw21I GWGCWC 2 cut(s) 168, 672
Alw26I GTCTC 2 cut(s) 303, 1074
AlwI GGATC 1 cut(s) 800
AlwNI CAGNNNCTG 1 cut(s) 486
Aor13HI TCCGGA 1 cut(s) 664
AoxI GGCC 5 cut(s) 85, 274, 488, 962, 981
ApeKI GCWGC 4 cut(s) 236, 311, 350, 967
ArsI GACNNNNNNTTYG 2 cut(s) 1001, 1033
AseI ATTAAT 1 cut(s) 1089
AspLEI GCGC 2 cut(s) 337, 690
AspS9I GGNCC 2 cut(s) 962, 982
AsuHPI GGTGA 2 cut(s) 643, 848
AsuII TTCGAA 1 cut(s) 892
BaeGI GKGCMC 1 cut(s) 707
BanI GGYRCC 2 cut(s) 687, 704
BanII GRGCYC 3 cut(s) 168, 380, 672
BbsI GAAGAC 1 cut(s) 768
Bbv12I GWGCWC 2 cut(s) 168, 672
BbvI GCAGC 4 cut(s) 223, 298, 337, 954
BccI CCATC 5 cut(s) 47, 602, 622, 722, 983
BcgI CGANNNNNNTGC 2 cut(s) 388, 422
BciT130I CCWGG 1 cut(s) 813
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 2 cut(s) 303, 1074
BfaI CTAG 1 cut(s) 605
BfoI RGCGCY 2 cut(s) 338, 691
BisI GCNGC 5 cut(s) 237, 312, 351, 965, 968
BlsI GCNGC 5 cut(s) 238, 313, 352, 966, 969
Bme1390I CCNGG 1 cut(s) 813
BmgT120I GGNCC 2 cut(s) 962, 982
BmiI GGNNCC 3 cut(s) 6, 689, 706
BmrFI CCNGG 1 cut(s) 813
BmsI GCATC 4 cut(s) 184, 298, 457, 463
BpiI GAAGAC 1 cut(s) 768
BpmI CTGGAG 3 cut(s) 314, 445, 834
Bpu14I TTCGAA 1 cut(s) 892
BpuEI CTTGAG 1 cut(s) 611
Bsa29I ATCGAT 2 cut(s) 177, 795
BsaAI YACGTR 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 797
BsaHI GRCGYC 1 cut(s) 688
BsaJI CCNNGG 4 cut(s) 240, 271, 616, 811
BsaWI WCCGGW 2 cut(s) 357, 664
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 664, 812, 1012
Bse1I ACTGG 2 cut(s) 462, 491
Bse3DI GCAATG 2 cut(s) 64, 1054
Bse8I GATNNNNATC 1 cut(s) 797
BseAI TCCGGA 1 cut(s) 664
BseBI CCWGG 1 cut(s) 813
BseCI ATCGAT 2 cut(s) 177, 795
BseDI CCNNGG 4 cut(s) 240, 271, 616, 811
BseGI GGATG 1 cut(s) 58
BseJI GATNNNNATC 1 cut(s) 797
BseLI CCNNNNNNNGG 4 cut(s) 283, 664, 812, 1012
BseMI GCAATG 2 cut(s) 64, 1054
BseMII CTCAG 2 cut(s) 181, 493
BseNI ACTGG 2 cut(s) 462, 491
BseSI GKGCMC 1 cut(s) 707
BseXI GCAGC 4 cut(s) 223, 298, 337, 954
BshFI GGCC 5 cut(s) 87, 276, 490, 964, 983
BshNI GGYRCC 2 cut(s) 687, 704
BshVI ATCGAT 2 cut(s) 177, 795
BsiHKAI GWGCWC 2 cut(s) 168, 672
BsiSI CCGG 5 cut(s) 338, 358, 410, 665, 971
BsiWI CGTACG 1 cut(s) 381
BslI CCNNNNNNNGG 4 cut(s) 283, 664, 812, 1012
BsmAI GTCTC 2 cut(s) 303, 1074
BsmI GAATGC 1 cut(s) 775
BsnI GGCC 5 cut(s) 87, 276, 490, 964, 983
Bsp119I TTCGAA 1 cut(s) 892
Bsp1286I GDGCHC 4 cut(s) 168, 380, 672, 707
Bsp13I TCCGGA 1 cut(s) 664
Bsp143I GATC 4 cut(s) 178, 202, 225, 792
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 2 cut(s) 197, 965
BspANI GGCC 5 cut(s) 87, 276, 490, 964, 983
BspCNI CTCAG 2 cut(s) 180, 492
BspDI ATCGAT 2 cut(s) 177, 795
BspEI TCCGGA 1 cut(s) 664
BspHI TCATGA 2 cut(s) 124, 205
BspLI GGNNCC 3 cut(s) 6, 689, 706
BspPI GGATC 1 cut(s) 800
BspT104I TTCGAA 1 cut(s) 892
BspT107I GGYRCC 2 cut(s) 687, 704
BsrDI GCAATG 2 cut(s) 64, 1054
BsrI ACTGG 2 cut(s) 462, 491
BssECI CCNNGG 4 cut(s) 240, 271, 616, 811
BssMI GATC 4 cut(s) 178, 202, 225, 792
BssNAI GTATAC 1 cut(s) 134
BssNI GRCGYC 1 cut(s) 688
BssT1I CCWWGG 2 cut(s) 271, 616
Bst1107I GTATAC 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 813
Bst4CI ACNGT 3 cut(s) 676, 715, 1007
BstACI GRCGYC 1 cut(s) 688
BstBAI YACGTR 1 cut(s) 385
BstBI TTCGAA 1 cut(s) 892
BstC8I GCNNGC 3 cut(s) 151, 278, 333
BstDEI CTNAG 5 cut(s) 82, 167, 186, 479, 854
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 1 cut(s) 58
BstH2I RGCGCY 2 cut(s) 338, 691
BstHHI GCGC 2 cut(s) 337, 690
BstKTI GATC 4 cut(s) 181, 205, 228, 795
BstMAI GTCTC 2 cut(s) 303, 1074
BstMBI GATC 4 cut(s) 178, 202, 225, 792
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 1 cut(s) 813
BstNSI RCATGY 1 cut(s) 657
BstSCI CCNGG 1 cut(s) 811
BstSLI GKGCMC 1 cut(s) 707
BstV1I GCAGC 4 cut(s) 223, 298, 337, 954
BstV2I GAAGAC 1 cut(s) 768
BstZ17I GTATAC 1 cut(s) 134
Bsu15I ATCGAT 2 cut(s) 177, 795
BsuRI GGCC 5 cut(s) 87, 276, 490, 964, 983
BsuTUI ATCGAT 2 cut(s) 177, 795
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 1 cut(s) 58
BtsIMutI CAGTG 3 cut(s) 469, 484, 672
Cac8I GCNNGC 3 cut(s) 151, 278, 333
CaiI CAGNNNCTG 1 cut(s) 486
CciI TCATGA 2 cut(s) 124, 205
CfoI GCGC 2 cut(s) 337, 690
Cfr13I GGNCC 2 cut(s) 962, 982
ClaI ATCGAT 2 cut(s) 177, 795
Csp6I GTAC 3 cut(s) 98, 382, 457
CviQI GTAC 3 cut(s) 98, 382, 457
DdeI CTNAG 5 cut(s) 82, 167, 186, 479, 854
DinI GGCGCC 1 cut(s) 689
DpnI GATC 4 cut(s) 180, 204, 227, 794
DpnII GATC 4 cut(s) 178, 202, 225, 792
Ecl136II GAGCTC 2 cut(s) 166, 670
Eco130I CCWWGG 2 cut(s) 271, 616
Eco147I AGGCCT 1 cut(s) 87
Eco24I GRGCYC 3 cut(s) 168, 380, 672
Eco32I GATATC 1 cut(s) 799
Eco53kI GAGCTC 2 cut(s) 166, 670
Eco57I CTGAAG 1 cut(s) 158
EcoICRI GAGCTC 2 cut(s) 166, 670
EcoRII CCWGG 1 cut(s) 811
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 2 cut(s) 271, 616
EcoT22I ATGCAT 1 cut(s) 540
EcoT38I GRGCYC 3 cut(s) 168, 380, 672
EgeI GGCGCC 1 cut(s) 689
EheI GGCGCC 1 cut(s) 689
ErhI CCWWGG 2 cut(s) 271, 616
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FauNDI CATATG 1 cut(s) 540
FbaI TGATCA 1 cut(s) 202
FblI GTMKAC 1 cut(s) 133
Fnu4HI GCNGC 5 cut(s) 237, 312, 351, 965, 968
FokI GGATG 1 cut(s) 65
FriOI GRGCYC 3 cut(s) 168, 380, 672
Fsp4HI GCNGC 5 cut(s) 237, 312, 351, 965, 968
FspBI CTAG 1 cut(s) 605
GlaI GCGC 2 cut(s) 336, 689
GluI GCNGC 5 cut(s) 237, 312, 351, 965, 968
GsuI CTGGAG 3 cut(s) 314, 445, 834
HaeII RGCGCY 2 cut(s) 338, 691
HaeIII GGCC 5 cut(s) 87, 276, 490, 964, 983
HapII CCGG 5 cut(s) 338, 358, 410, 665, 971
HhaI GCGC 2 cut(s) 337, 690
Hin1I GRCGYC 1 cut(s) 688
Hin6I GCGC 2 cut(s) 335, 688
HinP1I GCGC 2 cut(s) 335, 688
HindIII AAGCTT 2 cut(s) 140, 188
HinfI GANTC 4 cut(s) 296, 370, 596, 612
HpaII CCGG 5 cut(s) 338, 358, 410, 665, 971
HphI GGTGA 2 cut(s) 643, 848
Hpy166II GTNNAC 5 cut(s) 79, 134, 268, 457, 634
Hpy188I TCNGA 7 cut(s) 170, 303, 375, 581, 680, 697, 927
Hpy188III TCNNGA 5 cut(s) 125, 206, 665, 912, 1073
Hpy8I GTNNAC 5 cut(s) 79, 134, 268, 457, 634
HpyAV CCTTC 4 cut(s) 61, 415, 430, 745
HpyCH4III ACNGT 3 cut(s) 676, 715, 1007
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 7 cut(s) 149, 314, 501, 512, 538, 724, 1052
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 5 cut(s) 82, 167, 186, 479, 854
HpySE526I ACGT 1 cut(s) 384
Hsp92I GRCGYC 1 cut(s) 688
HspAI GCGC 2 cut(s) 335, 688
KasI GGCGCC 1 cut(s) 687
Kpn2I TCCGGA 1 cut(s) 664
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 4 cut(s) 178, 202, 225, 792
LmnI GCTCC 3 cut(s) 10, 667, 815
Lsp1109I GCAGC 4 cut(s) 223, 298, 337, 954
LweI GCATC 4 cut(s) 184, 298, 457, 463
MaeI CTAG 1 cut(s) 605
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 4 cut(s) 482, 583, 1001, 1007
MalI GATC 4 cut(s) 180, 204, 227, 794
MboI GATC 4 cut(s) 178, 202, 225, 792
MboII GAAGA 6 cut(s) 247, 316, 355, 770, 773, 876
MhlI GDGCHC 4 cut(s) 168, 380, 672, 707
MluCI AATT 3 cut(s) 732, 957, 1093
Mly113I GGCGCC 1 cut(s) 688
MlyI GAGTC 4 cut(s) 305, 379, 590, 606
MmeI TCCRAC 2 cut(s) 658, 971
MnlI CCTC 6 cut(s) 132, 235, 586, 813, 967, 1051
Mph1103I ATGCAT 1 cut(s) 540
MroI TCCGGA 1 cut(s) 664
MseI TTAA 6 cut(s) 144, 231, 525, 623, 710, 1089
MspA1I CMGCKG 1 cut(s) 967
MspI CCGG 5 cut(s) 338, 358, 410, 665, 971
MspR9I CCNGG 1 cut(s) 813
Mva1269I GAATGC 1 cut(s) 775
MvaI CCWGG 1 cut(s) 813
MwoI GCNNNNNNNGC 1 cut(s) 69
NarI GGCGCC 1 cut(s) 688
NcoI CCATGG 1 cut(s) 271
NdeI CATATG 1 cut(s) 540
NdeII GATC 4 cut(s) 178, 202, 225, 792
NlaIV GGNNCC 3 cut(s) 6, 689, 706
NmeAIII GCCGAG 1 cut(s) 265
NmuCI GTSAC 3 cut(s) 482, 583, 1007
NsiI ATGCAT 1 cut(s) 540
NspI RCATGY 1 cut(s) 657
NspV TTCGAA 1 cut(s) 892
PagI TCATGA 2 cut(s) 124, 205
PceI AGGCCT 1 cut(s) 87
PctI GAATGC 1 cut(s) 775
Pfl23II CGTACG 1 cut(s) 381
PkrI GCNGC 5 cut(s) 238, 313, 352, 966, 969
PleI GAGTC 4 cut(s) 304, 378, 590, 606
PluTI GGCGCC 1 cut(s) 691
PpsI GAGTC 4 cut(s) 304, 378, 590, 606
Ppu21I YACGTR 1 cut(s) 385
PshBI ATTAAT 1 cut(s) 1089
Psp124BI GAGCTC 2 cut(s) 168, 672
Psp6I CCWGG 1 cut(s) 811
PspGI CCWGG 1 cut(s) 811
PspLI CGTACG 1 cut(s) 381
PspN4I GGNNCC 3 cut(s) 6, 689, 706
PspPI GGNCC 2 cut(s) 962, 982
PsrI GAACNNNNNNTAC 2 cut(s) 96, 128
PstNI CAGNNNCTG 1 cut(s) 486
RsaI GTAC 3 cut(s) 99, 383, 458
RsaNI GTAC 3 cut(s) 98, 382, 457
SacI GAGCTC 2 cut(s) 168, 672
SaqAI TTAA 6 cut(s) 144, 231, 525, 623, 710, 1089
SatI GCNGC 5 cut(s) 237, 312, 351, 965, 968
Sau3AI GATC 4 cut(s) 178, 202, 225, 792
Sau96I GGNCC 2 cut(s) 962, 982
SchI GAGTC 4 cut(s) 305, 379, 590, 606
ScrFI CCNGG 1 cut(s) 813
SduI GDGCHC 4 cut(s) 168, 380, 672, 707
SfaNI GCATC 4 cut(s) 184, 298, 457, 463
SfoI GGCGCC 1 cut(s) 689
SfuI TTCGAA 1 cut(s) 892
SmlI CTYRAG 1 cut(s) 590
SmoI CTYRAG 1 cut(s) 590
Sse9I AATT 3 cut(s) 732, 957, 1093
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 2 cut(s) 197, 965
SspDI GGCGCC 1 cut(s) 687
SspMI CTAG 1 cut(s) 605
SstI GAGCTC 2 cut(s) 168, 672
StuI AGGCCT 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 811
StyI CCWWGG 2 cut(s) 271, 616
TaaI ACNGT 3 cut(s) 676, 715, 1007
TaiI ACGT 1 cut(s) 387
TaqI TCGA 4 cut(s) 177, 327, 795, 892
TasI AATT 3 cut(s) 732, 957, 1093
TatI WGTACW 1 cut(s) 456
TauI GCSGC 1 cut(s) 967
Tru1I TTAA 6 cut(s) 144, 231, 525, 623, 710, 1089
Tru9I TTAA 6 cut(s) 144, 231, 525, 623, 710, 1089
TscAI CASTG 3 cut(s) 469, 491, 679
TseFI GTSAC 3 cut(s) 482, 583, 1007
TseI GCWGC 4 cut(s) 236, 311, 350, 967
Tsp45I GTSAC 3 cut(s) 482, 583, 1007
TspDTI ATGAA 5 cut(s) 36, 222, 653, 782, 1046
TspRI CASTG 3 cut(s) 469, 491, 679
VspI ATTAAT 1 cut(s) 1089
XceI RCATGY 1 cut(s) 657
XmiI GTMKAC 1 cut(s) 133
XspI CTAG 1 cut(s) 605
Zsp2I ATGCAT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.