Rh5CG200500

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0012769
Physical Location & Seq
Forward (+)
20144697 .. 20145624
928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG200500.1

Sequence Viewer

Length: 648 bp
ATGGTGTACTCCAGTGATGCTGATGCTCTCAGTCACTGGCCTAACGAGTGCAAGGAAGTTGCACTTGAGTACCTTAAAACATCAATGCGTATGGTAAAGAAAATAGTGAAAACTTTGATTGAGAAGCTCGGAGTGACACTTGAGGACTCAAAACTAGATGGACTCGTTGGGTTAAAGATGGTGAACATGAACTTTTACCCAACATGCCCTAATCCGGAGCTCACTGTCGGAGTTGGGCGTCACTCGGACATGGGCACCTTAACCGTTTTACTGCAAGATGGAATTGGTGGTTTGTATGTGAAGGTTGAAGAAGACATGAATGCTGGAAAGAAAGGAGAGTGGATCGAGATCCCTCCTATCCCTGGAGCTTTGGTCATCAACATTGGTGATACATTACAGATACTTAGTAATGGGAGATATATAAGTGCCGAACACAGAGTTCGAACTACAAACGCTCAATCAAGAGTGTCTGTTCCGATATTTACCATTCCAAATCCAACAGAAATAATTGGGCCGCTGCCGGAGGTGGTGGCGCGTGATGGAGTGGCTCGTTACCGTGACATGGTTTTTGGAGATTATATGAACAACTTTTTCGGCAATGCACATGAGGGCAAGAAGTCTCTTGATTATGCCCAGATTAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

215

Amino Acids

23.77

Weight (kDa)

5.61

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 60 - 162 1.3e-28 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12900
fragaria_vesca FvH4_3g15580
malus_domestica MD00G1188900.v1.1 MD05G1221300.v1.1
prunus_persica Prupe.4G138300_v2.0.a1
pyrus_communis pycom05g20470
rosa_chinensis RchiOBHm_Chr5g0026211
rosa_laevigata RLG00000032912
rosa_multiflora Rmu_co8420971.1_g000001 Rmu_sc0002909.1_g000002 Rmu_sc0005061.1_g000001
rosa_roxburghii Rroxscaffold_1G00053100
rosa_rugosa Rorug05G0091600
rosa_samantha Rh5AG184500 Rh5BG181400 Rh5CG200500 Rh5DG183100
rosa_wichuraiana Rw5G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 254
AccII CGCG 1 cut(s) 535
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 1 cut(s) 515
AclWI GGATC 2 cut(s) 343, 350
AcyI GRCGYC 1 cut(s) 238
AfaI GTAC 2 cut(s) 8, 71
AfiI CCNNNNNNNGG 3 cut(s) 214, 362, 562
AgsI TTSAA 1 cut(s) 308
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 3 cut(s) 127, 220, 368
AluI AGCT 3 cut(s) 127, 220, 368
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 1 cut(s) 624
AlwI GGATC 2 cut(s) 343, 350
AlwNI CAGNNNCTG 1 cut(s) 36
Aor13HI TCCGGA 1 cut(s) 214
AoxI GGCC 2 cut(s) 38, 512
ApeKI GCWGC 1 cut(s) 517
ArsI GACNNNNNNTTYG 2 cut(s) 551, 583
AseI ATTAAT 1 cut(s) 639
AspLEI GCGC 1 cut(s) 535
AspS9I GGNCC 1 cut(s) 512
AsuHPI GGTGA 2 cut(s) 193, 398
AsuII TTCGAA 1 cut(s) 442
BaeGI GKGCMC 1 cut(s) 257
BaeI ACNNNNGTAYC 2 cut(s) 53, 86
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 1 cut(s) 222
BbsI GAAGAC 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 504
BccI CCATC 4 cut(s) 152, 172, 272, 533
BciT130I CCWGG 1 cut(s) 363
BcoDI GTCTC 1 cut(s) 624
BfaI CTAG 1 cut(s) 155
BisI GCNGC 2 cut(s) 515, 518
BlsI GCNGC 2 cut(s) 516, 519
Bme1390I CCNGG 1 cut(s) 363
BmgT120I GGNCC 1 cut(s) 512
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 7, 13
BpiI GAAGAC 1 cut(s) 318
BpmI CTGGAG 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 442
BpuEI CTTGAG 2 cut(s) 86, 161
BsaBI GATNNNNATC 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 238
BsaJI CCNNGG 1 cut(s) 361
BsaWI WCCGGW 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 362, 562
Bse1I ACTGG 2 cut(s) 12, 41
Bse3DI GCAATG 1 cut(s) 604
Bse8I GATNNNNATC 1 cut(s) 347
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 361
BseJI GATNNNNATC 1 cut(s) 347
BseLI CCNNNNNNNGG 3 cut(s) 214, 362, 562
BseMI GCAATG 1 cut(s) 604
BseMII CTCAG 1 cut(s) 43
BseNI ACTGG 2 cut(s) 12, 41
BseSI GKGCMC 1 cut(s) 257
BseXI GCAGC 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 535
BshFI GGCC 2 cut(s) 40, 514
BshNI GGYRCC 1 cut(s) 254
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 2 cut(s) 215, 521
BslI CCNNNNNNNGG 3 cut(s) 214, 362, 562
BsmAI GTCTC 1 cut(s) 624
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 2 cut(s) 40, 514
Bsp119I TTCGAA 1 cut(s) 442
Bsp1286I GDGCHC 2 cut(s) 222, 257
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 2 cut(s) 342, 348
BspACI CCGC 1 cut(s) 515
BspANI GGCC 2 cut(s) 40, 514
BspCNI CTCAG 1 cut(s) 42
BspEI TCCGGA 1 cut(s) 214
BspFNI CGCG 1 cut(s) 535
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 2 cut(s) 343, 350
BspT104I TTCGAA 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 604
BsrI ACTGG 2 cut(s) 12, 41
BssECI CCNNGG 1 cut(s) 361
BssMI GATC 2 cut(s) 342, 348
BssNI GRCGYC 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 3 cut(s) 226, 265, 557
BstACI GRCGYC 1 cut(s) 238
BstBI TTCGAA 1 cut(s) 442
BstDEI CTNAG 2 cut(s) 29, 404
BstFNI CGCG 1 cut(s) 535
BstHHI GCGC 1 cut(s) 535
BstKTI GATC 2 cut(s) 345, 351
BstMAI GTCTC 1 cut(s) 624
BstMBI GATC 2 cut(s) 342, 348
BstNI CCWGG 1 cut(s) 363
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 361
BstSLI GKGCMC 1 cut(s) 257
BstUI CGCG 1 cut(s) 535
BstV1I GCAGC 1 cut(s) 504
BstV2I GAAGAC 1 cut(s) 318
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BsuRI GGCC 2 cut(s) 40, 514
BtsIMutI CAGTG 3 cut(s) 19, 34, 222
CaiI CAGNNNCTG 1 cut(s) 36
CfoI GCGC 1 cut(s) 535
Cfr13I GGNCC 1 cut(s) 512
CseI GACGC 1 cut(s) 227
Csp6I GTAC 2 cut(s) 7, 70
CviAII CATG 6 cut(s) 187, 204, 250, 316, 562, 605
CviJI RGCY 6 cut(s) 40, 127, 220, 368, 514, 548
CviKI_1 RGCY 6 cut(s) 40, 127, 220, 368, 514, 548
CviQI GTAC 2 cut(s) 7, 70
DdeI CTNAG 2 cut(s) 29, 404
DpnI GATC 2 cut(s) 344, 350
DpnII GATC 2 cut(s) 342, 348
Ecl136II GAGCTC 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 222
Eco53kI GAGCTC 1 cut(s) 220
EcoICRI GAGCTC 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 222
FaeI CATG 6 cut(s) 190, 207, 253, 319, 565, 608
FalI AAGNNNNNCTT 2 cut(s) 48, 80
FatI CATG 6 cut(s) 186, 203, 249, 315, 561, 604
Fnu4HI GCNGC 2 cut(s) 515, 518
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 2 cut(s) 515, 518
FspBI CTAG 1 cut(s) 155
GlaI GCGC 1 cut(s) 534
GluI GCNGC 2 cut(s) 515, 518
GsuI CTGGAG 1 cut(s) 384
HaeIII GGCC 2 cut(s) 40, 514
HapII CCGG 2 cut(s) 215, 521
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 535
Hin1I GRCGYC 1 cut(s) 238
Hin1II CATG 6 cut(s) 190, 207, 253, 319, 565, 608
Hin6I GCGC 1 cut(s) 533
HinP1I GCGC 1 cut(s) 533
HinfI GANTC 2 cut(s) 146, 162
HpaII CCGG 2 cut(s) 215, 521
HphI GGTGA 2 cut(s) 193, 398
Hpy166II GTNNAC 2 cut(s) 7, 184
Hpy188I TCNGA 4 cut(s) 131, 230, 247, 477
Hpy188III TCNNGA 4 cut(s) 215, 346, 462, 623
Hpy8I GTNNAC 2 cut(s) 7, 184
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 3 cut(s) 226, 265, 557
HpyCH4V TGCA 4 cut(s) 51, 62, 274, 602
HpyF3I CTNAG 2 cut(s) 29, 404
Hsp92I GRCGYC 1 cut(s) 238
Hsp92II CATG 6 cut(s) 190, 207, 253, 319, 565, 608
HspAI GCGC 1 cut(s) 533
Kpn2I TCCGGA 1 cut(s) 214
Kzo9I GATC 2 cut(s) 342, 348
LmnI GCTCC 2 cut(s) 217, 365
LpnPI CCDG 7 cut(s) 22, 25, 228, 309, 348, 375, 534
Lsp1109I GCAGC 1 cut(s) 504
LweI GCATC 2 cut(s) 7, 13
MaeI CTAG 1 cut(s) 155
MaeIII GTNAC 5 cut(s) 32, 133, 239, 551, 557
MalI GATC 2 cut(s) 344, 350
MboI GATC 2 cut(s) 342, 348
MboII GAAGA 2 cut(s) 320, 323
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 2 cut(s) 222, 257
MluCI AATT 3 cut(s) 282, 507, 643
MlyI GAGTC 2 cut(s) 140, 156
MmeI TCCRAC 2 cut(s) 208, 521
MnlI CCTC 4 cut(s) 136, 363, 517, 601
MroI TCCGGA 1 cut(s) 214
MseI TTAA 4 cut(s) 75, 173, 260, 639
MspA1I CMGCKG 1 cut(s) 517
MspI CCGG 2 cut(s) 215, 521
MspR9I CCNGG 1 cut(s) 363
Mva1269I GAATGC 1 cut(s) 325
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 535
NdeII GATC 2 cut(s) 342, 348
NlaIII CATG 6 cut(s) 190, 207, 253, 319, 565, 608
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 4 cut(s) 32, 133, 239, 557
NspI RCATGY 1 cut(s) 207
NspV TTCGAA 1 cut(s) 442
PctI GAATGC 1 cut(s) 325
PkrI GCNGC 2 cut(s) 516, 519
PleI GAGTC 2 cut(s) 140, 156
PpsI GAGTC 2 cut(s) 140, 156
PshBI ATTAAT 1 cut(s) 639
Psp124BI GAGCTC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 512
PstNI CAGNNNCTG 1 cut(s) 36
PsuI RGATCY 1 cut(s) 348
RsaI GTAC 2 cut(s) 8, 71
RsaNI GTAC 2 cut(s) 7, 70
SacI GAGCTC 1 cut(s) 222
SaqAI TTAA 4 cut(s) 75, 173, 260, 639
SatI GCNGC 2 cut(s) 515, 518
Sau3AI GATC 2 cut(s) 342, 348
Sau96I GGNCC 1 cut(s) 512
SchI GAGTC 2 cut(s) 140, 156
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 2 cut(s) 222, 257
SetI ASST 7 cut(s) 75, 129, 222, 260, 306, 370, 528
SfaNI GCATC 2 cut(s) 7, 13
SfuI TTCGAA 1 cut(s) 442
SmlI CTYRAG 2 cut(s) 65, 140
SmoI CTYRAG 2 cut(s) 65, 140
Sse9I AATT 3 cut(s) 282, 507, 643
SsiI CCGC 1 cut(s) 515
SspMI CTAG 1 cut(s) 155
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 361
TaaI ACNGT 3 cut(s) 226, 265, 557
TaqI TCGA 2 cut(s) 345, 442
TasI AATT 3 cut(s) 282, 507, 643
TatI WGTACW 1 cut(s) 6
TauI GCSGC 1 cut(s) 517
Tru1I TTAA 4 cut(s) 75, 173, 260, 639
Tru9I TTAA 4 cut(s) 75, 173, 260, 639
TscAI CASTG 3 cut(s) 19, 41, 229
TseFI GTSAC 4 cut(s) 32, 133, 239, 557
TseI GCWGC 1 cut(s) 517
Tsp45I GTSAC 4 cut(s) 32, 133, 239, 557
TspDTI ATGAA 3 cut(s) 203, 332, 596
TspRI CASTG 3 cut(s) 19, 41, 229
VspI ATTAAT 1 cut(s) 639
XceI RCATGY 1 cut(s) 207
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.