MD00G1188900.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012769
Physical Location & Seq
Reverse (-)
44791638 .. 44793600
1963 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1188900.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGCTCCAAGTTTTGAAGACGGAAAATCACTATTCAATTTTGTTGTTCGAGATGGCAATGGTGTGAAGGGTATGCTGGATGGGCCCGATCATGACCAAGTGGCAAATGACTTTGCCAATGCCGCTGAGGTTCTTGGGTTCTTCCAAGTTTTGAACCATGGCATGCCTCTCGAGTTGCTAGAGTCCCTTAAAAGTTCTGCTAATGATTTCTTTGGACAAGCGCCGGAGAACAAGGCTGCGTATCGGAAAGGTGTGAGCCCTAGCCCATATGTGAAGTACGGAACAAGCTTTGTACCGGAGAACGAAAAGGCATTGGAGTGGAAGGACTATTTGAGCATGGTATATACCACTGATGCTGATGCTCTCAATTATTGGCCTAATGAATGCAAGGAAGTTGCACTTGAGTACCTAAAAACCTCAATAGATATGGTAGAGAAAATAGTGAAAGTCTTGATTACAAAGCTTGGAGTGATACTAGAAGACTCAAAACTTGAGGCACTCATTGGGTTAAGGATGGTTAACATGAATTATTACCCAACATGCCCTGATCCTGAGCTCACTGTGGGCATCGGGCGTCACTCAGACATGGGCAACCTAACTTGGCCTGAGATCACTCCTGTCCCTGGTGCTCTGATACTAAGTAATGGAAGGTACAAAAGTGCTGAACACAGAGCGCGAACTAAAAGCAGCCAATCAAGAGTGTCCGTCCCAATATTTACCATCCCAAAGGCAACAGAAAGAATTGGGCCATTGCCTGAGGTGGTGGAGAGTTATGGAGTTGCTCGTTATCGTGAGGTGGTGTTTGAGGATTATATGAACAACTTCTTTAGCATTGCACATGACGGGAAGAAGTCTCTTGATTATGCCACCAGATCATGCAAAAGATATCCAGTAATGTTAAACAAATTAAGTGACTGTGCAATTCAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

311

Amino Acids

34.65

Weight (kDa)

5.52

Isoelectric Point (pI)

31.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 27 - 127 6.1e-17 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12900
fragaria_vesca FvH4_3g15580
malus_domestica MD00G1188900.v1.1 MD05G1221300.v1.1
prunus_persica Prupe.4G138300_v2.0.a1
pyrus_communis pycom05g20470
rosa_chinensis RchiOBHm_Chr5g0026211
rosa_laevigata RLG00000032912
rosa_multiflora Rmu_co8420971.1_g000001 Rmu_sc0002909.1_g000002 Rmu_sc0005061.1_g000001
rosa_roxburghii Rroxscaffold_1G00053100
rosa_rugosa Rorug05G0091600
rosa_samantha Rh5AG184500 Rh5BG181400 Rh5CG200500 Rh5DG183100
rosa_wichuraiana Rw5G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 676
AciI CCGC 1 cut(s) 123
AclWI GGATC 1 cut(s) 542
AcyI GRCGYC 1 cut(s) 574
AfaI GTAC 4 cut(s) 278, 294, 407, 653
AfiI CCNNNNNNNGG 1 cut(s) 623
AgsI TTSAA 4 cut(s) 17, 37, 154, 926
AjnI CCWGG 1 cut(s) 622
AluBI AGCT 3 cut(s) 288, 463, 556
AluI AGCT 3 cut(s) 288, 463, 556
Alw21I GWGCWC 2 cut(s) 558, 631
Alw26I GTCTC 1 cut(s) 858
AlwI GGATC 1 cut(s) 542
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 4 cut(s) 83, 374, 602, 746
ApaI GGGCCC 1 cut(s) 87
ApeKI GCWGC 2 cut(s) 236, 687
Asp700I GAANNNNTTC 1 cut(s) 821
AspLEI GCGC 2 cut(s) 223, 676
AspS9I GGNCC 3 cut(s) 83, 84, 746
AvaI CYCGRG 1 cut(s) 170
AxyI CCTNAGG 1 cut(s) 756
BaeGI GKGCMC 1 cut(s) 87
BaeI ACNNNNGTAYC 4 cut(s) 389, 422, 643, 676
BanII GRGCYC 3 cut(s) 87, 260, 558
BbsI GAAGAC 2 cut(s) 24, 486
Bbv12I GWGCWC 2 cut(s) 558, 631
BbvCI CCTCAGC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 223, 699
BccI CCATC 4 cut(s) 47, 74, 508, 728
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciT130I CCWGG 1 cut(s) 624
BcoDI GTCTC 1 cut(s) 858
BfaI CTAG 3 cut(s) 179, 261, 476
BfoI RGCGCY 1 cut(s) 224
BisI GCNGC 3 cut(s) 123, 237, 688
BlsI GCNGC 3 cut(s) 124, 238, 689
Bme1390I CCNGG 1 cut(s) 624
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 3 cut(s) 83, 84, 746
BmiI GGNNCC 2 cut(s) 6, 85
BmrFI CCNGG 1 cut(s) 624
BmsI GCATC 3 cut(s) 343, 349, 576
BpiI GAAGAC 2 cut(s) 24, 486
Bpu10I CCTNAGC 2 cut(s) 126, 552
BpuEI CTTGAG 2 cut(s) 422, 512
BsaHI GRCGYC 1 cut(s) 574
BsaJI CCNNGG 2 cut(s) 157, 622
BsaWI WCCGGW 1 cut(s) 295
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse1I ACTGG 1 cut(s) 890
Bse21I CCTNAGG 1 cut(s) 756
Bse3DI GCAATG 3 cut(s) 64, 749, 831
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 2 cut(s) 157, 622
BseGI GGATG 3 cut(s) 85, 519, 720
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMI GCAATG 3 cut(s) 64, 749, 831
BseMII CTCAG 5 cut(s) 117, 543, 594, 597, 747
BseNI ACTGG 1 cut(s) 890
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 2 cut(s) 223, 699
Bsh1236I CGCG 1 cut(s) 676
BshFI GGCC 4 cut(s) 85, 376, 604, 748
BsiHKAI GWGCWC 2 cut(s) 558, 631
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 2 cut(s) 224, 296
BslFI GGGAC 3 cut(s) 169, 605, 692
BslI CCNNNNNNNGG 1 cut(s) 623
BsmAI GTCTC 1 cut(s) 858
BsmFI GGGAC 3 cut(s) 169, 605, 692
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 4 cut(s) 85, 376, 604, 748
BsoBI CYCGRG 1 cut(s) 170
Bsp120I GGGCCC 1 cut(s) 83
Bsp1286I GDGCHC 4 cut(s) 87, 260, 558, 631
Bsp143I GATC 4 cut(s) 88, 547, 609, 872
Bsp19I CCATGG 1 cut(s) 157
BspACI CCGC 1 cut(s) 123
BspANI GGCC 4 cut(s) 85, 376, 604, 748
BspCNI CTCAG 5 cut(s) 118, 544, 593, 598, 748
BspFNI CGCG 1 cut(s) 676
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 2 cut(s) 6, 85
BspPI GGATC 1 cut(s) 542
BsrDI GCAATG 3 cut(s) 64, 749, 831
BsrI ACTGG 1 cut(s) 890
BssECI CCNNGG 2 cut(s) 157, 622
BssMI GATC 4 cut(s) 88, 547, 609, 872
BssNI GRCGYC 1 cut(s) 574
BssT1I CCWWGG 1 cut(s) 157
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 2 cut(s) 562, 917
BstACI GRCGYC 1 cut(s) 574
BstC8I GCNNGC 1 cut(s) 164
BstDEI CTNAG 6 cut(s) 126, 552, 580, 606, 638, 756
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 3 cut(s) 85, 519, 720
BstFNI CGCG 1 cut(s) 676
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 2 cut(s) 223, 676
BstKTI GATC 4 cut(s) 91, 550, 612, 875
BstMAI GTCTC 1 cut(s) 858
BstMBI GATC 4 cut(s) 88, 547, 609, 872
BstMWI GCNNNNNNNGC 2 cut(s) 82, 122
BstNI CCWGG 1 cut(s) 624
BstNSI RCATGY 2 cut(s) 166, 543
BstSCI CCNGG 1 cut(s) 622
BstSLI GKGCMC 1 cut(s) 87
BstUI CGCG 1 cut(s) 676
BstV1I GCAGC 2 cut(s) 223, 699
BstV2I GAAGAC 2 cut(s) 24, 486
Bsu36I CCTNAGG 1 cut(s) 756
BsuRI GGCC 4 cut(s) 85, 376, 604, 748
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 3 cut(s) 85, 519, 720
BtsIMutI CAGTG 2 cut(s) 348, 558
Cac8I GCNNGC 1 cut(s) 164
CciI TCATGA 1 cut(s) 91
CfoI GCGC 2 cut(s) 223, 676
Cfr13I GGNCC 3 cut(s) 83, 84, 746
CseI GACGC 1 cut(s) 563
Csp6I GTAC 4 cut(s) 277, 293, 406, 652
CviAII CATG 9 cut(s) 92, 158, 163, 337, 523, 540, 586, 839, 876
CviQI GTAC 4 cut(s) 277, 293, 406, 652
DdeI CTNAG 6 cut(s) 126, 552, 580, 606, 638, 756
DpnI GATC 4 cut(s) 90, 549, 611, 874
DpnII GATC 4 cut(s) 88, 547, 609, 872
Ecl136II GAGCTC 1 cut(s) 556
Eco130I CCWWGG 1 cut(s) 157
Eco24I GRGCYC 3 cut(s) 87, 260, 558
Eco32I GATATC 1 cut(s) 887
Eco53kI GAGCTC 1 cut(s) 556
Eco81I CCTNAGG 1 cut(s) 756
Eco88I CYCGRG 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 556
EcoRII CCWGG 1 cut(s) 622
EcoRV GATATC 1 cut(s) 887
EcoT14I CCWWGG 1 cut(s) 157
EcoT38I GRGCYC 3 cut(s) 87, 260, 558
ErhI CCWWGG 1 cut(s) 157
FaeI CATG 9 cut(s) 95, 161, 166, 340, 526, 543, 589, 842, 879
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 3 cut(s) 169, 605, 692
FatI CATG 9 cut(s) 91, 157, 162, 336, 522, 539, 585, 838, 875
FauNDI CATATG 1 cut(s) 268
Fnu4HI GCNGC 3 cut(s) 123, 237, 688
FokI GGATG 3 cut(s) 92, 526, 707
FriOI GRGCYC 3 cut(s) 87, 260, 558
Fsp4HI GCNGC 3 cut(s) 123, 237, 688
FspBI CTAG 3 cut(s) 179, 261, 476
GlaI GCGC 2 cut(s) 222, 675
GluI GCNGC 3 cut(s) 123, 237, 688
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 4 cut(s) 85, 376, 604, 748
HapII CCGG 2 cut(s) 224, 296
HgaI GACGC 1 cut(s) 563
HhaI GCGC 2 cut(s) 223, 676
Hin1I GRCGYC 1 cut(s) 574
Hin1II CATG 9 cut(s) 95, 161, 166, 340, 526, 543, 589, 842, 879
Hin6I GCGC 2 cut(s) 221, 674
HinP1I GCGC 2 cut(s) 221, 674
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 2 cut(s) 286, 461
HinfI GANTC 2 cut(s) 182, 482
HpaI GTTAAC 1 cut(s) 520
HpaII CCGG 2 cut(s) 224, 296
Hpy166II GTNNAC 1 cut(s) 520
Hpy188I TCNGA 3 cut(s) 246, 583, 633
Hpy188III TCNNGA 8 cut(s) 50, 92, 170, 451, 551, 696, 791, 857
Hpy8I GTNNAC 1 cut(s) 520
HpyAV CCTTC 3 cut(s) 61, 316, 642
HpyCH4III ACNGT 2 cut(s) 562, 917
HpyCH4V TGCA 5 cut(s) 387, 398, 836, 879, 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 122
HpyF3I CTNAG 6 cut(s) 126, 552, 580, 606, 638, 756
Hsp92I GRCGYC 1 cut(s) 574
Hsp92II CATG 9 cut(s) 95, 161, 166, 340, 526, 543, 589, 842, 879
HspAI GCGC 2 cut(s) 221, 674
KspAI GTTAAC 1 cut(s) 520
Kzo9I GATC 4 cut(s) 88, 547, 609, 872
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 2 cut(s) 223, 699
LweI GCATC 3 cut(s) 343, 349, 576
MaeI CTAG 3 cut(s) 179, 261, 476
MaeIII GTNAC 2 cut(s) 575, 911
MalI GATC 4 cut(s) 90, 549, 611, 874
MboI GATC 4 cut(s) 88, 547, 609, 872
MboII GAAGA 4 cut(s) 29, 133, 491, 859
MhlI GDGCHC 4 cut(s) 87, 260, 558, 631
MluCI AATT 7 cut(s) 37, 367, 526, 741, 905, 921, 928
MlyI GAGTC 2 cut(s) 191, 476
MnlI CCTC 7 cut(s) 121, 177, 427, 487, 751, 787, 799
MroXI GAANNNNTTC 1 cut(s) 821
MseI TTAA 6 cut(s) 189, 509, 519, 899, 908, 931
MslI CAYNNNNRTG 1 cut(s) 316
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 2 cut(s) 224, 296
MspR9I CCNGG 1 cut(s) 624
Mva1269I GAATGC 1 cut(s) 389
MvaI CCWGG 1 cut(s) 624
MvnI CGCG 1 cut(s) 676
MwoI GCNNNNNNNGC 2 cut(s) 82, 122
NcoI CCATGG 1 cut(s) 157
NdeI CATATG 1 cut(s) 268
NdeII GATC 4 cut(s) 88, 547, 609, 872
NlaIII CATG 9 cut(s) 95, 161, 166, 340, 526, 543, 589, 842, 879
NlaIV GGNNCC 2 cut(s) 6, 85
NmuCI GTSAC 2 cut(s) 575, 911
NspI RCATGY 2 cut(s) 166, 543
PaeI GCATGC 1 cut(s) 166
PaeR7I CTCGAG 1 cut(s) 170
PagI TCATGA 1 cut(s) 91
PctI GAATGC 1 cut(s) 389
PdmI GAANNNNTTC 1 cut(s) 821
PkrI GCNGC 3 cut(s) 124, 238, 689
PleI GAGTC 2 cut(s) 190, 476
PpsI GAGTC 2 cut(s) 190, 476
Psp124BI GAGCTC 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
PspN4I GGNNCC 2 cut(s) 6, 85
PspOMI GGGCCC 1 cut(s) 83
PspPI GGNCC 3 cut(s) 83, 84, 746
RsaI GTAC 4 cut(s) 278, 294, 407, 653
RsaNI GTAC 4 cut(s) 277, 293, 406, 652
RseI CAYNNNNRTG 1 cut(s) 316
SacI GAGCTC 1 cut(s) 558
SaqAI TTAA 6 cut(s) 189, 509, 519, 899, 908, 931
SatI GCNGC 3 cut(s) 123, 237, 688
Sau3AI GATC 4 cut(s) 88, 547, 609, 872
Sau96I GGNCC 3 cut(s) 83, 84, 746
SchI GAGTC 2 cut(s) 191, 476
ScrFI CCNGG 1 cut(s) 624
SduI GDGCHC 4 cut(s) 87, 260, 558, 631
SfaNI GCATC 3 cut(s) 343, 349, 576
Sfr274I CTCGAG 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 316
SmlI CTYRAG 3 cut(s) 170, 401, 491
SmoI CTYRAG 3 cut(s) 170, 401, 491
SphI GCATGC 1 cut(s) 166
Sse9I AATT 7 cut(s) 37, 367, 526, 741, 905, 921, 928
SsiI CCGC 1 cut(s) 123
SspI AATATT 1 cut(s) 714
SspMI CTAG 3 cut(s) 179, 261, 476
SstI GAGCTC 1 cut(s) 558
StyD4I CCNGG 1 cut(s) 622
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 2 cut(s) 562, 917
TaqI TCGA 2 cut(s) 49, 171
TasI AATT 7 cut(s) 37, 367, 526, 741, 905, 921, 928
TauI GCSGC 1 cut(s) 125
Tru1I TTAA 6 cut(s) 189, 509, 519, 899, 908, 931
Tru9I TTAA 6 cut(s) 189, 509, 519, 899, 908, 931
TscAI CASTG 2 cut(s) 355, 565
TseFI GTSAC 2 cut(s) 575, 911
TseI GCWGC 2 cut(s) 236, 687
Tsp45I GTSAC 2 cut(s) 575, 911
TspDTI ATGAA 3 cut(s) 396, 539, 830
TspGWI ACGGA 3 cut(s) 36, 294, 694
TspRI CASTG 2 cut(s) 355, 565
XceI RCATGY 2 cut(s) 166, 543
XhoI CTCGAG 1 cut(s) 170
XmnI GAANNNNTTC 1 cut(s) 821
XspI CTAG 3 cut(s) 179, 261, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.