RLG00000032912

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012769
Physical Location & Seq
Forward (+)
20721196 .. 20722721
1526 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032912

Sequence Viewer

Length: 786 bp
ATGCCGCCTAGCCCTGCAGCTTATTCCATTTCCAGTCCGGGACTCCGAGGAGCCACCACTCTTCGCCGGGTCACTCGCCCCGACCCGGCACCGATTTTCATTAGCTATCTCTCTCTCATTTTCAATTGCAGAGCCTCAAGTGTTAGGATAAGGTCGAGCAGGAAAAAGGAAATGGTGAGAGGCAGGAGCAGGAAGGACGAGTCGGTGGTGCTGAACAGCATGAACGTGTTTGTGGCTCAGGGGAGCTTGAAGAAAAAGGAAGTTGCACTTGAGTACCTTAAAACATCAATGCATATGGTAAAGAAAATAGTGAAAACTTTGATTGAGAAGCTTGGAGTGACACTTGAGGACTCAAAACTAGATGGACTCGTTGGGTTAAAGATGGTGAACATGAACTTTTACCCAACATGCCCTAATCTGGAGCTCAATGTCAGAGTTGGGCGTCACTCGGACATGGGCACCTTAACCGGTGAAGAAGACATGAATGCTGGAAAGAAAGGAGAGTGGATCGAGATCCCTCCTATCCCTGGAGCTTTGATACTTAGTAATGGGAGATATAAAAGTGCCGAACACAAAGTTCGAACTACAAACGCTCAATCAAGAGTGTCTATTCCGATATTTACCATTCCAAATCCAACAGAAATAATTGGGCCGCTGTCGGAGGTGGTGGTGCGTGATGGAGTGGCTCGTTACCGTGACATGGTTTTTGGAGATTATATGAACAACTTTTTCTGCAATGCACATGAGGGCAAGAAGTCTCTTGATTATGCCCATATTAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

262

Amino Acids

29.07

Weight (kDa)

9.73

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 128 - 208 3.1e-11 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12900
fragaria_vesca FvH4_3g15580
malus_domestica MD00G1188900.v1.1 MD05G1221300.v1.1
prunus_persica Prupe.4G138300_v2.0.a1
pyrus_communis pycom05g20470
rosa_chinensis RchiOBHm_Chr5g0026211
rosa_laevigata RLG00000032912
rosa_multiflora Rmu_co8420971.1_g000001 Rmu_sc0002909.1_g000002 Rmu_sc0005061.1_g000001
rosa_roxburghii Rroxscaffold_1G00053100
rosa_rugosa Rorug05G0091600
rosa_samantha Rh5AG184500 Rh5BG181400 Rh5CG200500 Rh5DG183100
rosa_wichuraiana Rw5G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 88, 458
AciI CCGC 2 cut(s) 5, 653
AclWI GGATC 2 cut(s) 508, 515
AcyI GRCGYC 1 cut(s) 442
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 4 cut(s) 85, 418, 527, 700
AflIII ACRYGT 1 cut(s) 225
AgeI ACCGGT 1 cut(s) 467
AgsI TTSAA 2 cut(s) 124, 250
AjnI CCWGG 1 cut(s) 526
AluBI AGCT 6 cut(s) 20, 105, 246, 331, 424, 533
AluI AGCT 6 cut(s) 20, 105, 246, 331, 424, 533
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 1 cut(s) 762
AlwI GGATC 2 cut(s) 508, 515
AoxI GGCC 1 cut(s) 650
ApeKI GCWGC 1 cut(s) 17
ArsI GACNNNNNNTTYG 2 cut(s) 689, 721
AseI ATTAAT 1 cut(s) 777
AsiGI ACCGGT 1 cut(s) 467
AspS9I GGNCC 1 cut(s) 650
AsuC2I CCSGG 3 cut(s) 39, 68, 86
AsuHPI GGTGA 3 cut(s) 187, 397, 482
AsuII TTCGAA 1 cut(s) 580
BaeGI GKGCMC 1 cut(s) 461
BaeI ACNNNNGTAYC 2 cut(s) 257, 290
BanI GGYRCC 2 cut(s) 88, 458
BanII GRGCYC 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 483
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 1 cut(s) 29
BccI CCATC 3 cut(s) 356, 376, 671
BciT130I CCWGG 1 cut(s) 528
BcnI CCSGG 3 cut(s) 39, 68, 86
BcoDI GTCTC 1 cut(s) 762
BfaI CTAG 2 cut(s) 9, 359
BfmI CTRYAG 1 cut(s) 15
BisI GCNGC 3 cut(s) 5, 18, 653
BlsI GCNGC 3 cut(s) 6, 19, 654
Bme1390I CCNGG 4 cut(s) 39, 68, 86, 528
BmgT120I GGNCC 1 cut(s) 650
BmiI GGNNCC 3 cut(s) 52, 90, 460
BmrFI CCNGG 4 cut(s) 39, 68, 86, 528
BpiI GAAGAC 1 cut(s) 483
BpmI CTGGAG 2 cut(s) 440, 549
Bpu10I CCTNAGC 1 cut(s) 237
Bpu14I TTCGAA 1 cut(s) 580
BpuEI CTTGAG 3 cut(s) 121, 290, 365
BpuMI CCSGG 3 cut(s) 39, 68, 86
BsaBI GATNNNNATC 1 cut(s) 512
BsaHI GRCGYC 1 cut(s) 442
BsaJI CCNNGG 2 cut(s) 46, 526
BsaWI WCCGGW 1 cut(s) 467
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 418, 527, 700
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 1 cut(s) 33
Bse3DI GCAATG 1 cut(s) 742
Bse8I GATNNNNATC 1 cut(s) 512
BseBI CCWGG 1 cut(s) 528
BseDI CCNNGG 2 cut(s) 46, 526
BseJI GATNNNNATC 1 cut(s) 512
BseLI CCNNNNNNNGG 4 cut(s) 85, 418, 527, 700
BseMI GCAATG 1 cut(s) 742
BseMII CTCAG 1 cut(s) 251
BseNI ACTGG 1 cut(s) 33
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 461
BseXI GCAGC 1 cut(s) 29
BshFI GGCC 1 cut(s) 652
BshNI GGYRCC 2 cut(s) 88, 458
BshTI ACCGGT 1 cut(s) 467
BsiHKAI GWGCWC 1 cut(s) 426
BsiSI CCGG 4 cut(s) 38, 67, 86, 468
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 4 cut(s) 85, 418, 527, 700
BsmAI GTCTC 1 cut(s) 762
BsmFI GGGAC 1 cut(s) 54
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 1 cut(s) 652
Bsp119I TTCGAA 1 cut(s) 580
Bsp1286I GDGCHC 2 cut(s) 426, 461
Bsp143I GATC 2 cut(s) 507, 513
BspACI CCGC 2 cut(s) 5, 653
BspANI GGCC 1 cut(s) 652
BspCNI CTCAG 1 cut(s) 250
BspLI GGNNCC 3 cut(s) 52, 90, 460
BspMAI CTGCAG 1 cut(s) 19
BspPI GGATC 2 cut(s) 508, 515
BspT104I TTCGAA 1 cut(s) 580
BspT107I GGYRCC 2 cut(s) 88, 458
BsrDI GCAATG 1 cut(s) 742
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 1 cut(s) 33
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 2 cut(s) 46, 526
BssMI GATC 2 cut(s) 507, 513
BssNI GRCGYC 1 cut(s) 442
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 1 cut(s) 695
Bst6I CTCTTC 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 442
BstBI TTCGAA 1 cut(s) 580
BstDEI CTNAG 2 cut(s) 237, 542
BstKTI GATC 2 cut(s) 510, 516
BstMAI GTCTC 1 cut(s) 762
BstMBI GATC 2 cut(s) 507, 513
BstNI CCWGG 1 cut(s) 528
BstNSI RCATGY 1 cut(s) 411
BstSCI CCNGG 4 cut(s) 37, 66, 84, 526
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 1 cut(s) 461
BstV1I GCAGC 1 cut(s) 29
BstV2I GAAGAC 1 cut(s) 483
BstX2I RGATCY 1 cut(s) 513
BstYI RGATCY 1 cut(s) 513
BsuRI GGCC 1 cut(s) 652
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 1 cut(s) 650
CseI GACGC 1 cut(s) 431
Csp6I GTAC 1 cut(s) 274
CspAI ACCGGT 1 cut(s) 467
CviAII CATG 7 cut(s) 220, 391, 408, 454, 481, 700, 743
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 2 cut(s) 237, 542
DpnI GATC 2 cut(s) 509, 515
DpnII GATC 2 cut(s) 507, 513
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
Ecl136II GAGCTC 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 426
Eco53kI GAGCTC 1 cut(s) 424
EcoICRI GAGCTC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 526
EcoT22I ATGCAT 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 426
FaeI CATG 7 cut(s) 223, 394, 411, 457, 484, 703, 746
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FaqI GGGAC 1 cut(s) 54
FatI CATG 7 cut(s) 219, 390, 407, 453, 480, 699, 742
FauNDI CATATG 1 cut(s) 294
Fnu4HI GCNGC 3 cut(s) 5, 18, 653
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 3 cut(s) 5, 18, 653
FspBI CTAG 2 cut(s) 9, 359
GluI GCNGC 3 cut(s) 5, 18, 653
GsuI CTGGAG 2 cut(s) 440, 549
HaeIII GGCC 1 cut(s) 652
HapII CCGG 4 cut(s) 38, 67, 86, 468
HgaI GACGC 1 cut(s) 431
Hin1I GRCGYC 1 cut(s) 442
Hin1II CATG 7 cut(s) 223, 394, 411, 457, 484, 703, 746
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 4 cut(s) 42, 200, 350, 366
HpaII CCGG 4 cut(s) 38, 67, 86, 468
HphI GGTGA 3 cut(s) 187, 397, 482
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 5 cut(s) 47, 434, 451, 615, 661
Hpy188III TCNNGA 4 cut(s) 419, 511, 600, 761
Hpy8I GTNNAC 1 cut(s) 388
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 1 cut(s) 695
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 6 cut(s) 17, 129, 266, 292, 735, 740
HpyF3I CTNAG 2 cut(s) 237, 542
HpySE526I ACGT 1 cut(s) 225
Hsp92I GRCGYC 1 cut(s) 442
Hsp92II CATG 7 cut(s) 223, 394, 411, 457, 484, 703, 746
Kzo9I GATC 2 cut(s) 507, 513
LmnI GCTCC 5 cut(s) 50, 186, 243, 421, 530
Lsp1109I GCAGC 1 cut(s) 29
MaeI CTAG 2 cut(s) 9, 359
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 5 cut(s) 70, 337, 443, 689, 695
MalI GATC 2 cut(s) 509, 515
MboI GATC 2 cut(s) 507, 513
MboII GAAGA 4 cut(s) 53, 262, 485, 488
MfeI CAATTG 1 cut(s) 124
MflI RGATCY 1 cut(s) 513
MhlI GDGCHC 2 cut(s) 426, 461
MluCI AATT 3 cut(s) 124, 645, 781
MlyI GAGTC 4 cut(s) 36, 209, 344, 360
MmeI TCCRAC 2 cut(s) 639, 659
MnlI CCTC 7 cut(s) 41, 145, 173, 340, 528, 655, 739
Mph1103I ATGCAT 1 cut(s) 294
MseI TTAA 4 cut(s) 279, 377, 464, 777
MslI CAYNNNNRTG 1 cut(s) 224
MspA1I CMGCKG 1 cut(s) 655
MspI CCGG 4 cut(s) 38, 67, 86, 468
MspR9I CCNGG 4 cut(s) 39, 68, 86, 528
MunI CAATTG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 1 cut(s) 528
NciI CCSGG 3 cut(s) 39, 68, 86
NdeI CATATG 1 cut(s) 294
NdeII GATC 2 cut(s) 507, 513
NlaIII CATG 7 cut(s) 223, 394, 411, 457, 484, 703, 746
NlaIV GGNNCC 3 cut(s) 52, 90, 460
NmuCI GTSAC 4 cut(s) 70, 337, 443, 695
NsiI ATGCAT 1 cut(s) 294
NspI RCATGY 1 cut(s) 411
NspV TTCGAA 1 cut(s) 580
PctI GAATGC 1 cut(s) 490
PfoI TCCNGGA 1 cut(s) 37
PinAI ACCGGT 1 cut(s) 467
PkrI GCNGC 3 cut(s) 6, 19, 654
PleI GAGTC 4 cut(s) 36, 208, 344, 360
PpsI GAGTC 4 cut(s) 36, 208, 344, 360
PshBI ATTAAT 1 cut(s) 777
Psp124BI GAGCTC 1 cut(s) 426
Psp6I CCWGG 1 cut(s) 526
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 3 cut(s) 52, 90, 460
PspPI GGNCC 1 cut(s) 650
PstI CTGCAG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 513
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 224
SacI GAGCTC 1 cut(s) 426
SaqAI TTAA 4 cut(s) 279, 377, 464, 777
SatI GCNGC 3 cut(s) 5, 18, 653
Sau3AI GATC 2 cut(s) 507, 513
Sau96I GGNCC 1 cut(s) 650
SchI GAGTC 4 cut(s) 36, 209, 344, 360
ScrFI CCNGG 4 cut(s) 39, 68, 86, 528
SduI GDGCHC 2 cut(s) 426, 461
SfcI CTRYAG 1 cut(s) 15
SfuI TTCGAA 1 cut(s) 580
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 3 cut(s) 136, 269, 344
SmoI CTYRAG 3 cut(s) 136, 269, 344
Sse9I AATT 3 cut(s) 124, 645, 781
SsiI CCGC 2 cut(s) 5, 653
SspMI CTAG 2 cut(s) 9, 359
SstI GAGCTC 1 cut(s) 426
StyD4I CCNGG 4 cut(s) 37, 66, 84, 526
TaaI ACNGT 1 cut(s) 695
TaiI ACGT 1 cut(s) 228
TaqI TCGA 3 cut(s) 155, 510, 580
TasI AATT 3 cut(s) 124, 645, 781
TauI GCSGC 2 cut(s) 7, 655
Tru1I TTAA 4 cut(s) 279, 377, 464, 777
Tru9I TTAA 4 cut(s) 279, 377, 464, 777
TseFI GTSAC 4 cut(s) 70, 337, 443, 695
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 4 cut(s) 70, 337, 443, 695
TspDTI ATGAA 5 cut(s) 88, 236, 407, 497, 734
VspI ATTAAT 1 cut(s) 777
XceI RCATGY 1 cut(s) 411
XspI CTAG 2 cut(s) 9, 359
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.