MD05G1221300.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012769
Physical Location & Seq
Reverse (-)
35411620 .. 35413610
1991 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1221300.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGGCTCCTAGTTTTGAAGATGGAAAATCACTATTCAACTTTGTTGTTCGAGATGGCAATGGTGTGAAGGGCATGGTGGACTTAGGCTTGTCCAAAGTCCCTGAGCAATACATACAGCCACCACATGAGCGCATAGTGAAGCTGAATGCATCAGTTCATGAGCTACCACCAATTGATTTGTCAAAGCTGGATGGACCTGATCATGACCAAGTGGCAAATGACATTGCCAATGCTGCTGAGGTTCTTGGGTTCTTCCAAGTTTTGAACCATGGCATACCTCTCGAGTTGCTAGAATCCCTTAAAAGTTCTGCTAATGATTTCTTCGGACAAGCGCCTGAGAAGAAGGCTGCGTTTCAGAAAGGTTTGAGCCCTAGCCCGCACGTAAAGTATGGGACGAGCTTTGTGCCGGAGAAGGAGAAGGCATTGGAGTGGAAGGACTATGTGAGCATGGTGTATACTAGTGATGCTGATGCTCTCAGTTATTGGCCCAATGAATGCAAGAACGTTGCACTCGAGTATCTGAAAACCTCAACAGATATGGTAAAGAAAATAGTGAAAGTTTTGATAGCAAAGCTTGGAGTGACACTAGAAGACTCAAAATTTGAGGCACTCATTGGGTTAAGGATGGTTAACATGAATTATTACCCAACATGCCCTGATCCTGAGCTCACTGTTGGTGTCGGGCGTCACTCAGACATGGGCACCCTAACTGTGTTGCTGCAAGATGGAATTGGAGGTTTGTATGTGAAACTTGAAGAAGACATGGATGGTGAAAAGAAAGGAGAATGGATTGAGATCACTCCTGTCCCTGGTGCTCTGGTCATCAACATTGGTGATACGTTACAGATACTTAGTAATGGAAGGTACAAAAGTGCTGAGCACAGAGTGCGAACTACAAGCAGCCAATCAAGAGTGTCTGTCCCAATATTTAGCATCCCAAAGCCAACAGAAAGAATTGGGCCATTGCCTGAGGTGGTGGAAAGCGATGGAGTTGCTCGTTACCGTGAGGTGGTGTTTGAGGATTATATGAACAACTTCTTTAGCAATGCGCATGACGGGAAGAAGTCTCTTGATTACGCCACTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

363

Amino Acids

40.03

Weight (kDa)

5.17

Isoelectric Point (pI)

28.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 55 - 165 8.3e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 209 - 311 9.2e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12900
fragaria_vesca FvH4_3g15580
malus_domestica MD00G1188900.v1.1 MD05G1221300.v1.1
prunus_persica Prupe.4G138300_v2.0.a1
pyrus_communis pycom05g20470
rosa_chinensis RchiOBHm_Chr5g0026211
rosa_laevigata RLG00000032912
rosa_multiflora Rmu_co8420971.1_g000001 Rmu_sc0002909.1_g000002 Rmu_sc0005061.1_g000001
rosa_roxburghii Rroxscaffold_1G00053100
rosa_rugosa Rorug05G0091600
rosa_samantha Rh5AG184500 Rh5BG181400 Rh5CG200500 Rh5DG183100
rosa_wichuraiana Rw5G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1050
AccB1I GGYRCC 1 cut(s) 701
AccI GTMKAC 1 cut(s) 455
AciI CCGC 1 cut(s) 377
AclI AACGTT 1 cut(s) 504
AclWI GGATC 1 cut(s) 653
AcsI RAATTY 1 cut(s) 599
AcyI GRCGYC 1 cut(s) 685
AdeI CACNNNGTG 1 cut(s) 886
AfaI GTAC 1 cut(s) 866
AfiI CCNNNNNNNGG 2 cut(s) 809, 1009
AgsI TTSAA 4 cut(s) 17, 37, 265, 755
AhlI ACTAGT 1 cut(s) 458
AjnI CCWGG 1 cut(s) 808
AluBI AGCT 6 cut(s) 142, 163, 187, 399, 574, 667
AluI AGCT 6 cut(s) 142, 163, 187, 399, 574, 667
Alw21I GWGCWC 3 cut(s) 669, 817, 882
Alw26I GTCTC 1 cut(s) 1071
AlwI GGATC 1 cut(s) 653
Ama87I CYCGRG 2 cut(s) 281, 512
AoxI GGCC 2 cut(s) 485, 959
ApeKI GCWGC 4 cut(s) 233, 347, 718, 900
ApoI RAATTY 1 cut(s) 599
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
Asp700I GAANNNNTTC 1 cut(s) 1034
AspLEI GCGC 3 cut(s) 132, 334, 1051
AspS9I GGNCC 3 cut(s) 194, 486, 959
AsuHPI GGTGA 2 cut(s) 782, 845
AvaI CYCGRG 2 cut(s) 281, 512
AvaII GGWCC 1 cut(s) 194
AxyI CCTNAGG 1 cut(s) 969
BaeGI GKGCMC 1 cut(s) 704
BaeI ACNNNNGTAYC 4 cut(s) 500, 533, 856, 889
BanI GGYRCC 1 cut(s) 701
BanII GRGCYC 2 cut(s) 371, 669
BbsI GAAGAC 2 cut(s) 597, 765
Bbv12I GWGCWC 3 cut(s) 669, 817, 882
BbvCI CCTCAGC 1 cut(s) 237
BbvI GCAGC 4 cut(s) 220, 334, 705, 912
BccI CCATC 7 cut(s) 14, 47, 185, 619, 719, 761, 980
BcgI CGANNNNNNTGC 6 cut(s) 262, 296, 385, 419, 974, 1008
BciT130I CCWGG 1 cut(s) 810
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 1071
BcuI ACTAGT 1 cut(s) 458
BfaI CTAG 5 cut(s) 9, 290, 372, 459, 587
BfoI RGCGCY 1 cut(s) 335
BisI GCNGC 4 cut(s) 234, 348, 719, 901
BlpI GCTNAGC 1 cut(s) 876
BlsI GCNGC 4 cut(s) 235, 349, 720, 902
Bme1390I CCNGG 1 cut(s) 810
Bme18I GGWCC 1 cut(s) 194
BmeT110I CYCGRG 2 cut(s) 281, 512
BmgT120I GGNCC 3 cut(s) 194, 486, 959
BmiI GGNNCC 2 cut(s) 6, 703
BmrFI CCNGG 1 cut(s) 810
BmsI GCATC 4 cut(s) 158, 454, 460, 942
BpiI GAAGAC 2 cut(s) 597, 765
Bpu10I CCTNAGC 3 cut(s) 102, 237, 663
Bpu1102I GCTNAGC 1 cut(s) 876
BsaAI YACGTR 1 cut(s) 382
BsaBI GATNNNNATC 1 cut(s) 794
BsaHI GRCGYC 1 cut(s) 685
BsaJI CCNNGG 2 cut(s) 268, 808
Bsc4I CCNNNNNNNGG 2 cut(s) 809, 1009
Bse21I CCTNAGG 1 cut(s) 969
Bse3DI GCAATG 4 cut(s) 64, 222, 962, 1051
Bse8I GATNNNNATC 1 cut(s) 794
BseBI CCWGG 1 cut(s) 810
BseDI CCNNGG 2 cut(s) 268, 808
BseGI GGATG 4 cut(s) 196, 630, 772, 933
BseJI GATNNNNATC 1 cut(s) 794
BseLI CCNNNNNNNGG 2 cut(s) 809, 1009
BseMI GCAATG 4 cut(s) 64, 222, 962, 1051
BseMII CTCAG 8 cut(s) 93, 228, 327, 490, 654, 705, 867, 960
BseSI GKGCMC 1 cut(s) 704
BseXI GCAGC 4 cut(s) 220, 334, 705, 912
BshFI GGCC 2 cut(s) 487, 961
BshNI GGYRCC 1 cut(s) 701
BsiHKAI GWGCWC 3 cut(s) 669, 817, 882
BsiHKCI CYCGRG 2 cut(s) 281, 512
BsiSI CCGG 1 cut(s) 407
BslFI GGGAC 4 cut(s) 83, 406, 791, 905
BslI CCNNNNNNNGG 2 cut(s) 809, 1009
BsmAI GTCTC 1 cut(s) 1071
BsmFI GGGAC 4 cut(s) 83, 406, 791, 905
BsmI GAATGC 2 cut(s) 151, 500
BsnI GGCC 2 cut(s) 487, 961
BsoBI CYCGRG 2 cut(s) 281, 512
Bsp1286I GDGCHC 5 cut(s) 371, 669, 704, 817, 882
Bsp143I GATC 3 cut(s) 199, 658, 795
Bsp1720I GCTNAGC 1 cut(s) 876
Bsp19I CCATGG 1 cut(s) 268
BspACI CCGC 1 cut(s) 377
BspANI GGCC 2 cut(s) 487, 961
BspCNI CTCAG 8 cut(s) 94, 229, 328, 489, 655, 704, 868, 961
BspHI TCATGA 2 cut(s) 157, 202
BspLI GGNNCC 2 cut(s) 6, 703
BspPI GGATC 1 cut(s) 653
BspT107I GGYRCC 1 cut(s) 701
BsrDI GCAATG 4 cut(s) 64, 222, 962, 1051
BssECI CCNNGG 2 cut(s) 268, 808
BssMI GATC 3 cut(s) 199, 658, 795
BssNAI GTATAC 1 cut(s) 456
BssNI GRCGYC 1 cut(s) 685
BssT1I CCWWGG 1 cut(s) 268
Bst1107I GTATAC 1 cut(s) 456
Bst2UI CCWGG 1 cut(s) 810
Bst4CI ACNGT 3 cut(s) 673, 712, 1004
BstACI GRCGYC 1 cut(s) 685
BstAPI GCANNNNNTGC 1 cut(s) 886
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 1 cut(s) 377
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 4 cut(s) 196, 630, 772, 933
BstH2I RGCGCY 1 cut(s) 335
BstHHI GCGC 3 cut(s) 132, 334, 1051
BstKTI GATC 3 cut(s) 202, 661, 798
BstMAI GTCTC 1 cut(s) 1071
BstMBI GATC 3 cut(s) 199, 658, 795
BstMWI GCNNNNNNNGC 2 cut(s) 233, 886
BstNI CCWGG 1 cut(s) 810
BstNSI RCATGY 1 cut(s) 654
BstSCI CCNGG 1 cut(s) 808
BstSLI GKGCMC 1 cut(s) 704
BstV1I GCAGC 4 cut(s) 220, 334, 705, 912
BstV2I GAAGAC 2 cut(s) 597, 765
BstZ17I GTATAC 1 cut(s) 456
Bsu36I CCTNAGG 1 cut(s) 969
BsuRI GGCC 2 cut(s) 487, 961
BtgI CCRYGG 1 cut(s) 268
BtgZI GCGATG 1 cut(s) 999
BtsCI GGATG 4 cut(s) 196, 630, 772, 933
BtsIMutI CAGTG 1 cut(s) 669
Cac8I GCNNGC 1 cut(s) 377
CciI TCATGA 2 cut(s) 157, 202
CfoI GCGC 3 cut(s) 132, 334, 1051
Cfr13I GGNCC 3 cut(s) 194, 486, 959
CseI GACGC 1 cut(s) 674
Csp6I GTAC 1 cut(s) 865
CviQI GTAC 1 cut(s) 865
DpnI GATC 3 cut(s) 201, 660, 797
DpnII GATC 3 cut(s) 199, 658, 795
DraIII CACNNNGTG 1 cut(s) 886
Ecl136II GAGCTC 1 cut(s) 667
Eco130I CCWWGG 1 cut(s) 268
Eco24I GRGCYC 2 cut(s) 371, 669
Eco47I GGWCC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 667
Eco81I CCTNAGG 1 cut(s) 969
Eco88I CYCGRG 2 cut(s) 281, 512
EcoICRI GAGCTC 1 cut(s) 667
EcoRII CCWGG 1 cut(s) 808
EcoT14I CCWWGG 1 cut(s) 268
EcoT22I ATGCAT 1 cut(s) 151
EcoT38I GRGCYC 2 cut(s) 371, 669
ErhI CCWWGG 1 cut(s) 268
FaqI GGGAC 4 cut(s) 83, 406, 791, 905
FauI CCCGC 1 cut(s) 384
FbaI TGATCA 1 cut(s) 199
FblI GTMKAC 1 cut(s) 455
Fnu4HI GCNGC 4 cut(s) 234, 348, 719, 901
FokI GGATG 4 cut(s) 203, 637, 779, 920
FriOI GRGCYC 2 cut(s) 371, 669
Fsp4HI GCNGC 4 cut(s) 234, 348, 719, 901
FspAI RTGCGCAY 1 cut(s) 1050
FspBI CTAG 5 cut(s) 9, 290, 372, 459, 587
FspI TGCGCA 1 cut(s) 1050
GlaI GCGC 3 cut(s) 131, 333, 1050
GluI GCNGC 4 cut(s) 234, 348, 719, 901
HaeII RGCGCY 1 cut(s) 335
HaeIII GGCC 2 cut(s) 487, 961
HapII CCGG 1 cut(s) 407
HgaI GACGC 1 cut(s) 674
HhaI GCGC 3 cut(s) 132, 334, 1051
Hin1I GRCGYC 1 cut(s) 685
Hin6I GCGC 3 cut(s) 130, 332, 1049
HinP1I GCGC 3 cut(s) 130, 332, 1049
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 2 cut(s) 293, 593
HpaI GTTAAC 1 cut(s) 631
HpaII CCGG 1 cut(s) 407
HphI GGTGA 2 cut(s) 782, 845
Hpy166II GTNNAC 3 cut(s) 79, 456, 631
Hpy188I TCNGA 4 cut(s) 326, 357, 522, 694
Hpy188III TCNNGA 7 cut(s) 50, 158, 203, 281, 662, 909, 1070
Hpy8I GTNNAC 3 cut(s) 79, 456, 631
HpyAV CCTTC 6 cut(s) 61, 337, 406, 412, 427, 855
HpyCH4III ACNGT 3 cut(s) 673, 712, 1004
HpyCH4IV ACGT 3 cut(s) 381, 504, 839
HpyCH4V TGCA 4 cut(s) 149, 498, 509, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 886
HpySE526I ACGT 3 cut(s) 381, 504, 839
Hsp92I GRCGYC 1 cut(s) 685
HspAI GCGC 3 cut(s) 130, 332, 1049
Ksp22I TGATCA 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 631
Kzo9I GATC 3 cut(s) 199, 658, 795
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 4 cut(s) 220, 334, 705, 912
LweI GCATC 4 cut(s) 158, 454, 460, 942
MaeI CTAG 5 cut(s) 9, 290, 372, 459, 587
MaeII ACGT 3 cut(s) 381, 504, 839
MaeIII GTNAC 4 cut(s) 580, 686, 840, 998
MalI GATC 3 cut(s) 201, 660, 797
MboI GATC 3 cut(s) 199, 658, 795
MboII GAAGA 8 cut(s) 29, 244, 313, 352, 602, 767, 770, 1072
MfeI CAATTG 1 cut(s) 171
MhlI GDGCHC 5 cut(s) 371, 669, 704, 817, 882
MluCI AATT 5 cut(s) 171, 599, 637, 729, 954
MlyI GAGTC 1 cut(s) 587
MnlI CCTC 8 cut(s) 232, 288, 538, 598, 728, 964, 1000, 1012
Mph1103I ATGCAT 1 cut(s) 151
MroXI GAANNNNTTC 1 cut(s) 1034
MseI TTAA 3 cut(s) 300, 620, 630
MslI CAYNNNNRTG 1 cut(s) 427
MspI CCGG 1 cut(s) 407
MspR9I CCNGG 1 cut(s) 810
MunI CAATTG 1 cut(s) 171
Mva1269I GAATGC 2 cut(s) 151, 500
MvaI CCWGG 1 cut(s) 810
MwoI GCNNNNNNNGC 2 cut(s) 233, 886
NcoI CCATGG 1 cut(s) 268
NdeII GATC 3 cut(s) 199, 658, 795
NlaIV GGNNCC 2 cut(s) 6, 703
NmuCI GTSAC 2 cut(s) 580, 686
NsbI TGCGCA 1 cut(s) 1050
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 1 cut(s) 654
PaeR7I CTCGAG 2 cut(s) 281, 512
PagI TCATGA 2 cut(s) 157, 202
PcsI WCGNNNNNNNCGW 1 cut(s) 510
PctI GAATGC 2 cut(s) 151, 500
PdmI GAANNNNTTC 1 cut(s) 1034
PfeI GAWTC 1 cut(s) 293
PkrI GCNGC 4 cut(s) 235, 349, 720, 902
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
Ppu21I YACGTR 1 cut(s) 382
Psp124BI GAGCTC 1 cut(s) 669
Psp1406I AACGTT 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 808
PspGI CCWGG 1 cut(s) 808
PspN4I GGNNCC 2 cut(s) 6, 703
PspPI GGNCC 3 cut(s) 194, 486, 959
PspXI VCTCGAGB 1 cut(s) 512
RsaI GTAC 1 cut(s) 866
RsaNI GTAC 1 cut(s) 865
RseI CAYNNNNRTG 1 cut(s) 427
SacI GAGCTC 1 cut(s) 669
SaqAI TTAA 3 cut(s) 300, 620, 630
SatI GCNGC 4 cut(s) 234, 348, 719, 901
Sau3AI GATC 3 cut(s) 199, 658, 795
Sau96I GGNCC 3 cut(s) 194, 486, 959
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 1 cut(s) 810
SduI GDGCHC 5 cut(s) 371, 669, 704, 817, 882
SfaNI GCATC 4 cut(s) 158, 454, 460, 942
Sfr274I CTCGAG 2 cut(s) 281, 512
SinI GGWCC 1 cut(s) 194
SlaI CTCGAG 2 cut(s) 281, 512
SmiMI CAYNNNNRTG 1 cut(s) 427
SmlI CTYRAG 2 cut(s) 281, 512
SmoI CTYRAG 2 cut(s) 281, 512
SpeI ACTAGT 1 cut(s) 458
Sse9I AATT 5 cut(s) 171, 599, 637, 729, 954
SsiI CCGC 1 cut(s) 377
SspI AATATT 1 cut(s) 927
SspMI CTAG 5 cut(s) 9, 290, 372, 459, 587
SstI GAGCTC 1 cut(s) 669
StyD4I CCNGG 1 cut(s) 808
StyI CCWWGG 1 cut(s) 268
TaaI ACNGT 3 cut(s) 673, 712, 1004
TaiI ACGT 3 cut(s) 384, 507, 842
TaqI TCGA 3 cut(s) 49, 282, 513
TasI AATT 5 cut(s) 171, 599, 637, 729, 954
TfiI GAWTC 1 cut(s) 293
Tru1I TTAA 3 cut(s) 300, 620, 630
Tru9I TTAA 3 cut(s) 300, 620, 630
TscAI CASTG 1 cut(s) 676
TseFI GTSAC 2 cut(s) 580, 686
TseI GCWGC 4 cut(s) 233, 347, 718, 900
Tsp45I GTSAC 2 cut(s) 580, 686
TspDTI ATGAA 4 cut(s) 146, 507, 650, 1043
TspRI CASTG 1 cut(s) 676
VpaK11BI GGWCC 1 cut(s) 194
XapI RAATTY 1 cut(s) 599
XceI RCATGY 1 cut(s) 654
XhoI CTCGAG 2 cut(s) 281, 512
XmiI GTMKAC 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 1034
XspI CTAG 5 cut(s) 9, 290, 372, 459, 587
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.