Rh5BG181400

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012769
Physical Location & Seq
Forward (+)
19906633 .. 19907999
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG181400.1

Sequence Viewer

Length: 1071 bp
ATGGCTCCAAGTTTCAATGATGAAAACTCACTATTCAACTTTGTTGTCAGGGATGGCAATGGCGTGAAGGGCATGGTGGACTTAGGCCTATCCAAAGTACCTGAACAATACATACAACCACCTCATGAGCGTATACTGAAGCTTAATGCAAGCTCACTAAATGAGCTCAGACCAATCGATCTGTCTAAGCTTGATGCGGTTGATCATGAACAAGTGGCTAATGAGATCGTTAATGCTGCCGAGGTTCTTGGGTTCTTCCAAGTTATGAACCATGGCCTACCTGTGGAGTTGCTGGAGTCTCTGAAAGATGCCGCAAACAAGTTCTTCGAGCAAGTGCCGGAGAAGAAAGCTGCTTACTGGAAAGGTGTGAGCCCGAGCCTGTACGTGAAGTATGGAACGAGTTTTGTGCCGGAGAAAGAGAAGGCTTTGGAGTGGAAGGACTATGTTAGCATGGTGTACTCCAGTGATGCTGATGCTCTCAGTCACTGGCCTAACGAGTGCAAGGAAGTTGCACTTGAGTACCTTAAAACATCAATGCATATGGTAAAGAAAATAGTGAAAACTTTGATTGAGAAGCTCGGAGTGACACTTGAGGACTCAAAACTAGATGGACTCGTTGGGTTAAAGATGGTGAACATGAACTTTTACCCAACATGCCCTAATCCGGAGCTCACTGTCGGAGTTGGGCGTCACTCGGACATGGGTACCTTAACCGTTTTACTGCAAGATGGAATTGGTGGTTTGTATGTGAAGGTTGAAGAAGACATGAATGCTGGAAAGAAAGGAGAGTGGATCGAGATCCCTCCTATCCCTGGAGCTTTGATACTTAGTAATGGGAGATATAAAAGTGCCGAACACAGAGTTCGAACTACAAACGCTCAATCAAGAGTGTCTGTTCCGATATTTACCATTCCAAATCCAACAGAAATAATTGGGCCGCTGCCGGTGGTGGTGGCGCGTGATGGAGTGGCTCGTTACCGTGACATGGTTTTTGGAGATTATATGAACAACTTTTTCGGCAATGCACATGAGGGCAAGAAGTCTCTTGATTATGCCCAGATTAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

356

Amino Acids

39.62

Weight (kDa)

5.59

Isoelectric Point (pI)

30.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 59 - 165 9.4e-20 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 210 - 303 7e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12900
fragaria_vesca FvH4_3g15580
malus_domestica MD00G1188900.v1.1 MD05G1221300.v1.1
prunus_persica Prupe.4G138300_v2.0.a1
pyrus_communis pycom05g20470
rosa_chinensis RchiOBHm_Chr5g0026211
rosa_laevigata RLG00000032912
rosa_multiflora Rmu_co8420971.1_g000001 Rmu_sc0002909.1_g000002 Rmu_sc0005061.1_g000001
rosa_roxburghii Rroxscaffold_1G00053100
rosa_rugosa Rorug05G0091600
rosa_samantha Rh5AG184500 Rh5BG181400 Rh5CG200500 Rh5DG183100
rosa_wichuraiana Rw5G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 704
AccB1I GGYRCC 1 cut(s) 704
AccI GTMKAC 1 cut(s) 133
AccII CGCG 1 cut(s) 958
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 3 cut(s) 197, 312, 938
AclWI GGATC 2 cut(s) 793, 800
AcuI CTGAAG 1 cut(s) 158
AcyI GRCGYC 1 cut(s) 688
AfaI GTAC 5 cut(s) 99, 383, 458, 521, 706
AfiI CCNNNNNNNGG 4 cut(s) 283, 664, 812, 985
AgsI TTSAA 3 cut(s) 16, 37, 758
AjnI CCWGG 1 cut(s) 811
AluBI AGCT 8 cut(s) 142, 153, 166, 190, 350, 577, 670, 818
AluI AGCT 8 cut(s) 142, 153, 166, 190, 350, 577, 670, 818
Alw21I GWGCWC 2 cut(s) 168, 672
Alw26I GTCTC 2 cut(s) 303, 1047
AlwI GGATC 2 cut(s) 793, 800
AlwNI CAGNNNCTG 1 cut(s) 486
Ama87I CYCGRG 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 664
AoxI GGCC 4 cut(s) 85, 274, 488, 935
ApeKI GCWGC 3 cut(s) 236, 350, 940
ArsI GACNNNNNNTTYG 2 cut(s) 974, 1006
AseI ATTAAT 1 cut(s) 1062
Asp718I GGTACC 1 cut(s) 704
AspLEI GCGC 1 cut(s) 958
AspS9I GGNCC 1 cut(s) 935
AsuHPI GGTGA 1 cut(s) 643
AsuII TTCGAA 1 cut(s) 865
AvaI CYCGRG 1 cut(s) 373
BaeI ACNNNNGTAYC 4 cut(s) 503, 536, 688, 721
BanI GGYRCC 1 cut(s) 704
BanII GRGCYC 3 cut(s) 168, 374, 672
BbsI GAAGAC 1 cut(s) 768
Bbv12I GWGCWC 2 cut(s) 168, 672
BbvI GCAGC 3 cut(s) 223, 337, 927
BccI CCATC 5 cut(s) 47, 602, 622, 722, 956
BcgI CGANNNNNNTGC 2 cut(s) 388, 422
BciT130I CCWGG 1 cut(s) 813
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 2 cut(s) 303, 1047
BfaI CTAG 1 cut(s) 605
BisI GCNGC 5 cut(s) 237, 312, 351, 938, 941
BlsI GCNGC 5 cut(s) 238, 313, 352, 939, 942
Bme1390I CCNGG 1 cut(s) 813
BmeT110I CYCGRG 1 cut(s) 373
BmgT120I GGNCC 1 cut(s) 935
BmiI GGNNCC 2 cut(s) 6, 706
BmrFI CCNGG 1 cut(s) 813
BmsI GCATC 4 cut(s) 184, 298, 457, 463
BpiI GAAGAC 1 cut(s) 768
BpmI CTGGAG 3 cut(s) 314, 445, 834
Bpu14I TTCGAA 1 cut(s) 865
BpuEI CTTGAG 2 cut(s) 536, 611
Bsa29I ATCGAT 1 cut(s) 177
BsaAI YACGTR 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 797
BsaHI GRCGYC 1 cut(s) 688
BsaJI CCNNGG 3 cut(s) 240, 271, 811
BsaWI WCCGGW 1 cut(s) 664
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 664, 812, 985
Bse118I RCCGGY 1 cut(s) 943
Bse1I ACTGG 3 cut(s) 362, 462, 491
Bse3DI GCAATG 2 cut(s) 64, 1027
Bse8I GATNNNNATC 1 cut(s) 797
BseAI TCCGGA 1 cut(s) 664
BseBI CCWGG 1 cut(s) 813
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 240, 271, 811
BseGI GGATG 1 cut(s) 58
BseJI GATNNNNATC 1 cut(s) 797
BseLI CCNNNNNNNGG 4 cut(s) 283, 664, 812, 985
BseMI GCAATG 2 cut(s) 64, 1027
BseMII CTCAG 2 cut(s) 181, 493
BseNI ACTGG 3 cut(s) 362, 462, 491
BseXI GCAGC 3 cut(s) 223, 337, 927
Bsh1236I CGCG 1 cut(s) 958
BshFI GGCC 4 cut(s) 87, 276, 490, 937
BshNI GGYRCC 1 cut(s) 704
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 2 cut(s) 168, 672
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 4 cut(s) 338, 410, 665, 944
BslI CCNNNNNNNGG 4 cut(s) 283, 664, 812, 985
BsmAI GTCTC 2 cut(s) 303, 1047
BsmI GAATGC 1 cut(s) 775
BsnI GGCC 4 cut(s) 87, 276, 490, 937
BsoBI CYCGRG 1 cut(s) 373
Bsp119I TTCGAA 1 cut(s) 865
Bsp1286I GDGCHC 3 cut(s) 168, 374, 672
Bsp13I TCCGGA 1 cut(s) 664
Bsp143I GATC 5 cut(s) 178, 202, 225, 792, 798
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 3 cut(s) 197, 312, 938
BspANI GGCC 4 cut(s) 87, 276, 490, 937
BspCNI CTCAG 2 cut(s) 180, 492
BspDI ATCGAT 1 cut(s) 177
BspEI TCCGGA 1 cut(s) 664
BspFNI CGCG 1 cut(s) 958
BspHI TCATGA 2 cut(s) 124, 205
BspLI GGNNCC 2 cut(s) 6, 706
BspPI GGATC 2 cut(s) 793, 800
BspT104I TTCGAA 1 cut(s) 865
BspT107I GGYRCC 1 cut(s) 704
BsrDI GCAATG 2 cut(s) 64, 1027
BsrFI RCCGGY 1 cut(s) 943
BsrI ACTGG 3 cut(s) 362, 462, 491
BssAI RCCGGY 1 cut(s) 943
BssECI CCNNGG 3 cut(s) 240, 271, 811
BssMI GATC 5 cut(s) 178, 202, 225, 792, 798
BssNAI GTATAC 1 cut(s) 134
BssNI GRCGYC 1 cut(s) 688
BssT1I CCWWGG 1 cut(s) 271
Bst1107I GTATAC 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 813
Bst4CI ACNGT 3 cut(s) 676, 715, 980
BstACI GRCGYC 1 cut(s) 688
BstBAI YACGTR 1 cut(s) 385
BstBI TTCGAA 1 cut(s) 865
BstC8I GCNNGC 1 cut(s) 151
BstDEI CTNAG 5 cut(s) 82, 167, 186, 479, 827
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 1 cut(s) 58
BstFNI CGCG 1 cut(s) 958
BstHHI GCGC 1 cut(s) 958
BstKTI GATC 5 cut(s) 181, 205, 228, 795, 801
BstMAI GTCTC 2 cut(s) 303, 1047
BstMBI GATC 5 cut(s) 178, 202, 225, 792, 798
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 1 cut(s) 813
BstNSI RCATGY 1 cut(s) 657
BstSCI CCNGG 1 cut(s) 811
BstUI CGCG 1 cut(s) 958
BstV1I GCAGC 3 cut(s) 223, 337, 927
BstV2I GAAGAC 1 cut(s) 768
BstX2I RGATCY 1 cut(s) 798
BstYI RGATCY 1 cut(s) 798
BstZ17I GTATAC 1 cut(s) 134
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 4 cut(s) 87, 276, 490, 937
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 1 cut(s) 58
BtsIMutI CAGTG 3 cut(s) 469, 484, 672
Cac8I GCNNGC 1 cut(s) 151
CaiI CAGNNNCTG 1 cut(s) 486
CciI TCATGA 2 cut(s) 124, 205
CfoI GCGC 1 cut(s) 958
Cfr10I RCCGGY 1 cut(s) 943
Cfr13I GGNCC 1 cut(s) 935
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 1 cut(s) 677
Csp6I GTAC 5 cut(s) 98, 382, 457, 520, 705
CviQI GTAC 5 cut(s) 98, 382, 457, 520, 705
DdeI CTNAG 5 cut(s) 82, 167, 186, 479, 827
DpnI GATC 5 cut(s) 180, 204, 227, 794, 800
DpnII GATC 5 cut(s) 178, 202, 225, 792, 798
Ecl136II GAGCTC 2 cut(s) 166, 670
Eco130I CCWWGG 1 cut(s) 271
Eco147I AGGCCT 1 cut(s) 87
Eco24I GRGCYC 3 cut(s) 168, 374, 672
Eco53kI GAGCTC 2 cut(s) 166, 670
Eco57I CTGAAG 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 373
EcoICRI GAGCTC 2 cut(s) 166, 670
EcoRII CCWGG 1 cut(s) 811
EcoT14I CCWWGG 1 cut(s) 271
EcoT22I ATGCAT 1 cut(s) 540
EcoT38I GRGCYC 3 cut(s) 168, 374, 672
ErhI CCWWGG 1 cut(s) 271
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FauNDI CATATG 1 cut(s) 540
FbaI TGATCA 1 cut(s) 202
FblI GTMKAC 1 cut(s) 133
Fnu4HI GCNGC 5 cut(s) 237, 312, 351, 938, 941
FokI GGATG 1 cut(s) 65
FriOI GRGCYC 3 cut(s) 168, 374, 672
Fsp4HI GCNGC 5 cut(s) 237, 312, 351, 938, 941
FspBI CTAG 1 cut(s) 605
GlaI GCGC 1 cut(s) 957
GluI GCNGC 5 cut(s) 237, 312, 351, 938, 941
GsuI CTGGAG 3 cut(s) 314, 445, 834
HaeIII GGCC 4 cut(s) 87, 276, 490, 937
HapII CCGG 4 cut(s) 338, 410, 665, 944
HgaI GACGC 1 cut(s) 677
HhaI GCGC 1 cut(s) 958
Hin1I GRCGYC 1 cut(s) 688
Hin6I GCGC 1 cut(s) 956
HinP1I GCGC 1 cut(s) 956
HindIII AAGCTT 2 cut(s) 140, 188
HinfI GANTC 3 cut(s) 296, 596, 612
HpaII CCGG 4 cut(s) 338, 410, 665, 944
HphI GGTGA 1 cut(s) 643
Hpy166II GTNNAC 4 cut(s) 79, 134, 457, 634
Hpy188I TCNGA 6 cut(s) 170, 303, 581, 680, 697, 900
Hpy188III TCNNGA 6 cut(s) 125, 206, 665, 796, 885, 1046
Hpy8I GTNNAC 4 cut(s) 79, 134, 457, 634
HpyAV CCTTC 4 cut(s) 61, 415, 430, 745
HpyCH4III ACNGT 3 cut(s) 676, 715, 980
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 6 cut(s) 149, 501, 512, 538, 724, 1025
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 5 cut(s) 82, 167, 186, 479, 827
HpySE526I ACGT 1 cut(s) 384
Hsp92I GRCGYC 1 cut(s) 688
HspAI GCGC 1 cut(s) 956
Kpn2I TCCGGA 1 cut(s) 664
KpnI GGTACC 1 cut(s) 708
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 5 cut(s) 178, 202, 225, 792, 798
LmnI GCTCC 3 cut(s) 10, 667, 815
Lsp1109I GCAGC 3 cut(s) 223, 337, 927
LweI GCATC 4 cut(s) 184, 298, 457, 463
MaeI CTAG 1 cut(s) 605
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 5 cut(s) 482, 583, 689, 974, 980
MalI GATC 5 cut(s) 180, 204, 227, 794, 800
MboI GATC 5 cut(s) 178, 202, 225, 792, 798
MboII GAAGA 5 cut(s) 247, 316, 355, 770, 773
MflI RGATCY 1 cut(s) 798
MhlI GDGCHC 3 cut(s) 168, 374, 672
MluCI AATT 3 cut(s) 732, 930, 1066
MlyI GAGTC 3 cut(s) 305, 590, 606
MmeI TCCRAC 2 cut(s) 658, 944
MnlI CCTC 5 cut(s) 132, 235, 586, 813, 1024
Mph1103I ATGCAT 1 cut(s) 540
MroI TCCGGA 1 cut(s) 664
MseI TTAA 6 cut(s) 144, 231, 525, 623, 710, 1062
MspA1I CMGCKG 1 cut(s) 940
MspI CCGG 4 cut(s) 338, 410, 665, 944
MspR9I CCNGG 1 cut(s) 813
Mva1269I GAATGC 1 cut(s) 775
MvaI CCWGG 1 cut(s) 813
MvnI CGCG 1 cut(s) 958
MwoI GCNNNNNNNGC 1 cut(s) 69
NcoI CCATGG 1 cut(s) 271
NdeI CATATG 1 cut(s) 540
NdeII GATC 5 cut(s) 178, 202, 225, 792, 798
NlaIV GGNNCC 2 cut(s) 6, 706
NmeAIII GCCGAG 1 cut(s) 265
NmuCI GTSAC 4 cut(s) 482, 583, 689, 980
NsiI ATGCAT 1 cut(s) 540
NspI RCATGY 1 cut(s) 657
NspV TTCGAA 1 cut(s) 865
PagI TCATGA 2 cut(s) 124, 205
PceI AGGCCT 1 cut(s) 87
PctI GAATGC 1 cut(s) 775
PkrI GCNGC 5 cut(s) 238, 313, 352, 939, 942
PleI GAGTC 3 cut(s) 304, 590, 606
PpsI GAGTC 3 cut(s) 304, 590, 606
Ppu21I YACGTR 1 cut(s) 385
PshBI ATTAAT 1 cut(s) 1062
Psp124BI GAGCTC 2 cut(s) 168, 672
Psp6I CCWGG 1 cut(s) 811
PspGI CCWGG 1 cut(s) 811
PspN4I GGNNCC 2 cut(s) 6, 706
PspPI GGNCC 1 cut(s) 935
PsrI GAACNNNNNNTAC 2 cut(s) 96, 128
PstNI CAGNNNCTG 1 cut(s) 486
PsuI RGATCY 1 cut(s) 798
RsaI GTAC 5 cut(s) 99, 383, 458, 521, 706
RsaNI GTAC 5 cut(s) 98, 382, 457, 520, 705
SacI GAGCTC 2 cut(s) 168, 672
SaqAI TTAA 6 cut(s) 144, 231, 525, 623, 710, 1062
SatI GCNGC 5 cut(s) 237, 312, 351, 938, 941
Sau3AI GATC 5 cut(s) 178, 202, 225, 792, 798
Sau96I GGNCC 1 cut(s) 935
SchI GAGTC 3 cut(s) 305, 590, 606
ScrFI CCNGG 1 cut(s) 813
SduI GDGCHC 3 cut(s) 168, 374, 672
SfaNI GCATC 4 cut(s) 184, 298, 457, 463
SfuI TTCGAA 1 cut(s) 865
SmlI CTYRAG 2 cut(s) 515, 590
SmoI CTYRAG 2 cut(s) 515, 590
Sse9I AATT 3 cut(s) 732, 930, 1066
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 3 cut(s) 197, 312, 938
SspMI CTAG 1 cut(s) 605
SstI GAGCTC 2 cut(s) 168, 672
StuI AGGCCT 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 811
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 3 cut(s) 676, 715, 980
TaiI ACGT 1 cut(s) 387
TaqI TCGA 4 cut(s) 177, 327, 795, 865
TasI AATT 3 cut(s) 732, 930, 1066
TatI WGTACW 1 cut(s) 456
TauI GCSGC 2 cut(s) 314, 940
Tru1I TTAA 6 cut(s) 144, 231, 525, 623, 710, 1062
Tru9I TTAA 6 cut(s) 144, 231, 525, 623, 710, 1062
TscAI CASTG 3 cut(s) 469, 491, 679
TseFI GTSAC 4 cut(s) 482, 583, 689, 980
TseI GCWGC 3 cut(s) 236, 350, 940
Tsp45I GTSAC 4 cut(s) 482, 583, 689, 980
TspDTI ATGAA 6 cut(s) 36, 222, 281, 653, 782, 1019
TspRI CASTG 3 cut(s) 469, 491, 679
VspI ATTAAT 1 cut(s) 1062
XceI RCATGY 1 cut(s) 657
XmiI GTMKAC 1 cut(s) 133
XspI CTAG 1 cut(s) 605
Zsp2I ATGCAT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.