AT3G18240

Mitochondrial ribosomal subunit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006027
Physical Location & Seq
Forward (+)
6254842 .. 6258090
3249 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G18240.2

Sequence Viewer

Length: 1260 bp
ATGAGAGGAGCTCTCTTGAGAAATGCATCTCTCTGCGCTCGCAGAATCATTCTTTCTCCTCGTATAACCCATCAGATCTCCCCCAATGTTCCCTTTCTCGCTCCAATCGCCGCTCCTGCTGCTCCCAAGTTCAGATTTTTCTCCTCCGAATCCGGTGAGAACTCGACTACGGCCCCTGAGTCTTCGCCGACTGATTCACCCGAGAAGAAAGATCTCGTTGTAGAAGATGTAAGCAACAAAGAGCTTAAAAGCCGTATAGAGAAGTATTTCAATGAGGGTAATGAAGATGCTTTACCCGGAGTTATTGAAGCACTTCTACAGAGAAGACTTGTAGACAAACACGCAGAGACTGATGATGAATTGTTGGAAAAAATCGAGTCTCTACCTTTCAAAGATGATGTCAAGGATGAAGATTTTGAATCCGATTTCGAGGAAGCACATTCTACTGACGAAGAACTTGAGGATTTGTACAATTCTCCCGAGTATGTAGCTGAAAAGATGAGGAAGAATGAGTTTTTCAACATGGATGATAAGAAGTGGGATCACATGATTAGAGAAGGTATCCAACATGGGTGTCTTACTGATACTAAAGAATGCGAGGAGATTCTTGAGGATATGCTCAAATGGGACCAGCTTCTTCCTGATGACTTGAAGAAAAAGGTTGAAGCAAAGTTTAATGAGCTTGGAGATATGTGTGAAAGAGGTGAAATTGAGGCTGAAGCAGCTTATGAGCTTTTTAAGGAATTTGAAGATGAGATGGTAATTCAGTATGGGGATCAGATGGAAGCTGAGGGTCCTCCCCAATTTGGTGAAACAGATGCTTCCGATAGGAATACTGACTTGGATGATCCCCCTGGTAAAGGCCCAATCCTCAGGTGGCAATCAAGGATAGTTTTTGCTCCTGGAGGTGATGCGTGGCATCCAAAGAACAGGAAAGTTAAAATGTCTGTTACAGTGAAAGAGCTTGGGCTCTCAAAGCATCAGGCTAAAAGGCTAAGGGAACTTGTAGGGAAAAGATACCATTCGGGGAAAGACGAGCTTACAATCACCAGCGAGAGGTTTGAACACCGAGAGGAAAACAGGAAAGACTGCTTAAGAACTCTTTACGGGTTGATAGAAGAAGCTGGGAAAGCCAACAAGATCGCTGAAGACATAAGAACTTCATATGTAAAACAAAGACTCCGTGCCAATCCAGCTTTTATGCAGAAACTACAAGCCAAGATCATAAGGTCTAAAGAATCAGATGCCATCAACGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S28

Protein Analysis

419

Amino Acids

48.05

Weight (kDa)

4.98

Isoelectric Point (pI)

52.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S28 PF10213 291 - 385 2.2e-16 Mitochondrial ribosomal subunit protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 113
AccI GTMKAC 1 cut(s) 333
AciI CCGC 1 cut(s) 111
AclWI GGATC 3 cut(s) 549, 783, 842
AcsI RAATTY 1 cut(s) 743
AcuI CTGAAG 2 cut(s) 738, 1167
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 3 cut(s) 806, 860, 1056
AflII CTTAAG 1 cut(s) 1093
AgsI TTSAA 9 cut(s) 271, 308, 391, 419, 520, 652, 665, 749, 1064
AjnI CCWGG 2 cut(s) 853, 901
AjuI GAANNNNNNNTTGG 2 cut(s) 824, 856
Alw21I GWGCWC 1 cut(s) 13
Alw26I GTCTC 2 cut(s) 341, 384
AlwI GGATC 3 cut(s) 549, 783, 842
AlwNI CAGNNNCTG 1 cut(s) 350
Ama87I CYCGRG 2 cut(s) 200, 479
AoxI GGCC 2 cut(s) 171, 862
ApeKI GCWGC 2 cut(s) 119, 722
ApoI RAATTY 1 cut(s) 743
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
Asp700I GAANNNNTTC 2 cut(s) 266, 312
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 4 cut(s) 172, 628, 794, 863
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 6 cut(s) 167, 189, 716, 821, 920, 1039
AvaI CYCGRG 2 cut(s) 200, 479
AvaII GGWCC 2 cut(s) 628, 794
AxyI CCTNAGG 1 cut(s) 872
BanII GRGCYC 2 cut(s) 13, 972
BarI GAAGNNNNNNTAC 4 cut(s) 276, 300, 308, 332
BbsI GAAGAC 3 cut(s) 174, 331, 1155
Bbv12I GWGCWC 1 cut(s) 13
BbvCI CCTCAGC 1 cut(s) 789
BbvI GCAGC 2 cut(s) 106, 734
BccI CCATC 4 cut(s) 78, 751, 775, 1256
BceAI ACGGC 2 cut(s) 186, 237
BciT130I CCWGG 2 cut(s) 855, 903
BciVI GTATCC 1 cut(s) 572
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 2 cut(s) 341, 384
BfmI CTRYAG 1 cut(s) 317
BfrI CTTAAG 1 cut(s) 1093
BfuI GTATCC 1 cut(s) 572
BglII AGATCT 2 cut(s) 75, 211
BisI GCNGC 3 cut(s) 111, 120, 723
BlsI GCNGC 3 cut(s) 112, 121, 724
Bme1390I CCNGG 3 cut(s) 297, 855, 903
Bme18I GGWCC 2 cut(s) 628, 794
BmeT110I CYCGRG 2 cut(s) 200, 479
BmgT120I GGNCC 4 cut(s) 172, 628, 794, 863
BmiI GGNNCC 3 cut(s) 174, 629, 795
BmrFI CCNGG 3 cut(s) 297, 855, 903
BmsI GCATC 7 cut(s) 35, 277, 808, 901, 928, 988, 1234
BpiI GAAGAC 3 cut(s) 174, 331, 1155
BplI GAGNNNNNCTC 2 cut(s) 27, 29
BpmI CTGGAG 1 cut(s) 924
Bpu10I CCTNAGC 2 cut(s) 789, 995
BpuEI CTTGAG 3 cut(s) 37, 479, 629
BpuMI CCSGG 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 853
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 2 cut(s) 1164, 1194
Bsc4I CCNNNNNNNGG 3 cut(s) 806, 860, 1056
Bse21I CCTNAGG 1 cut(s) 872
BseBI CCWGG 2 cut(s) 855, 903
BseDI CCNNGG 1 cut(s) 853
BseGI GGATG 4 cut(s) 412, 532, 850, 919
BseLI CCNNNNNNNGG 3 cut(s) 806, 860, 1056
BseMII CTCAG 3 cut(s) 168, 780, 886
BseRI GAGGAG 4 cut(s) 21, 48, 133, 614
BseXI GCAGC 2 cut(s) 106, 734
BseYI CCCAGC 1 cut(s) 1124
BshFI GGCC 2 cut(s) 173, 864
BsiHKAI GWGCWC 1 cut(s) 13
BsiHKCI CYCGRG 2 cut(s) 200, 479
BsiSI CCGG 2 cut(s) 153, 297
BslFI GGGAC 1 cut(s) 641
BslI CCNNNNNNNGG 3 cut(s) 806, 860, 1056
BsmAI GTCTC 2 cut(s) 341, 384
BsmFI GGGAC 1 cut(s) 641
BsmI GAATGC 1 cut(s) 599
BsnI GGCC 2 cut(s) 173, 864
BsoBI CYCGRG 2 cut(s) 200, 479
Bsp1286I GDGCHC 2 cut(s) 13, 972
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 7 cut(s) 75, 211, 541, 775, 847, 1140, 1221
BspACI CCGC 1 cut(s) 111
BspANI GGCC 2 cut(s) 173, 864
BspCNI CTCAG 3 cut(s) 169, 781, 885
BspLI GGNNCC 3 cut(s) 174, 629, 795
BspPI GGATC 3 cut(s) 549, 783, 842
BspTI CTTAAG 1 cut(s) 1093
BsrBI CCGCTC 1 cut(s) 113
BsrGI TGTACA 1 cut(s) 468
BssECI CCNNGG 1 cut(s) 853
BssMI GATC 7 cut(s) 75, 211, 541, 775, 847, 1140, 1221
Bst2UI CCWGG 2 cut(s) 855, 903
Bst4CI ACNGT 1 cut(s) 955
BstAFI CTTAAG 1 cut(s) 1093
BstAUI TGTACA 1 cut(s) 468
BstC8I GCNNGC 1 cut(s) 40
BstDEI CTNAG 4 cut(s) 177, 789, 872, 995
BstENI CCTNNNNNAGG 1 cut(s) 858
BstF5I GGATG 4 cut(s) 412, 532, 850, 919
BstHHI GCGC 1 cut(s) 38
BstKTI GATC 7 cut(s) 78, 214, 544, 778, 850, 1143, 1224
BstMAI GTCTC 2 cut(s) 341, 384
BstMBI GATC 7 cut(s) 75, 211, 541, 775, 847, 1140, 1221
BstMWI GCNNNNNNNGC 8 cut(s) 107, 116, 119, 722, 976, 1130, 1193, 1253
BstNI CCWGG 2 cut(s) 855, 903
BstSCI CCNGG 3 cut(s) 295, 853, 901
BstSFI CTRYAG 1 cut(s) 317
BstV1I GCAGC 2 cut(s) 106, 734
BstV2I GAAGAC 3 cut(s) 174, 331, 1155
BstX2I RGATCY 2 cut(s) 75, 211
BstYI RGATCY 2 cut(s) 75, 211
Bsu36I CCTNAGG 1 cut(s) 872
BsuI GTATCC 1 cut(s) 572
BsuRI GGCC 2 cut(s) 173, 864
BtsCI GGATG 4 cut(s) 412, 532, 850, 919
BtsIMutI CAGTG 1 cut(s) 960
Cac8I GCNNGC 1 cut(s) 40
CaiI CAGNNNCTG 1 cut(s) 350
CfoI GCGC 1 cut(s) 38
Cfr13I GGNCC 4 cut(s) 172, 628, 794, 863
Csp6I GTAC 1 cut(s) 469
CviAII CATG 3 cut(s) 523, 547, 569
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 4 cut(s) 177, 789, 872, 995
DpnI GATC 7 cut(s) 77, 213, 543, 777, 849, 1142, 1223
DpnII GATC 7 cut(s) 75, 211, 541, 775, 847, 1140, 1221
Ecl136II GAGCTC 1 cut(s) 11
Eco24I GRGCYC 2 cut(s) 13, 972
Eco47I GGWCC 2 cut(s) 628, 794
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 2 cut(s) 738, 1167
Eco81I CCTNAGG 1 cut(s) 872
Eco88I CYCGRG 2 cut(s) 200, 479
EcoICRI GAGCTC 1 cut(s) 11
EcoNI CCTNNNNNAGG 1 cut(s) 858
EcoO109I RGGNCCY 1 cut(s) 794
EcoRII CCWGG 2 cut(s) 853, 901
EcoT22I ATGCAT 1 cut(s) 28
EcoT38I GRGCYC 2 cut(s) 13, 972
FaeI CATG 3 cut(s) 526, 550, 572
FalI AAGNNNNNCTT 2 cut(s) 1023, 1055
FaqI GGGAC 1 cut(s) 641
FatI CATG 3 cut(s) 522, 546, 568
FauNDI CATATG 1 cut(s) 1165
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 3 cut(s) 111, 120, 723
FokI GGATG 4 cut(s) 419, 539, 857, 906
FriOI GRGCYC 2 cut(s) 13, 972
Fsp4HI GCNGC 3 cut(s) 111, 120, 723
GlaI GCGC 1 cut(s) 37
GluI GCNGC 3 cut(s) 111, 120, 723
GsaI CCCAGC 1 cut(s) 1128
GsuI CTGGAG 1 cut(s) 924
HaeIII GGCC 2 cut(s) 173, 864
HapII CCGG 2 cut(s) 153, 297
HhaI GCGC 1 cut(s) 38
Hin1II CATG 3 cut(s) 526, 550, 572
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HinfI GANTC 9 cut(s) 45, 149, 179, 194, 377, 419, 604, 1179, 1238
HpaII CCGG 2 cut(s) 153, 297
HphI GGTGA 6 cut(s) 167, 189, 716, 821, 920, 1039
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 7 cut(s) 75, 134, 148, 424, 780, 826, 1243
Hpy188III TCNNGA 4 cut(s) 16, 479, 608, 641
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 1 cut(s) 551
HpyCH4III ACNGT 1 cut(s) 955
HpyCH4V TGCA 2 cut(s) 26, 1204
HpyF10VI GCNNNNNNNGC 8 cut(s) 107, 116, 119, 722, 976, 1130, 1193, 1253
HpyF3I CTNAG 4 cut(s) 177, 789, 872, 995
Hsp92II CATG 3 cut(s) 526, 550, 572
HspAI GCGC 1 cut(s) 36
Kzo9I GATC 7 cut(s) 75, 211, 541, 775, 847, 1140, 1221
LmnI GCTCC 5 cut(s) 8, 106, 118, 127, 904
Lsp1109I GCAGC 2 cut(s) 106, 734
LweI GCATC 7 cut(s) 35, 277, 808, 901, 928, 988, 1234
MaeIII GTNAC 1 cut(s) 949
MalI GATC 7 cut(s) 77, 213, 543, 777, 849, 1142, 1223
MbiI CCGCTC 1 cut(s) 113
MboI GATC 7 cut(s) 75, 211, 541, 775, 847, 1140, 1221
MflI RGATCY 2 cut(s) 75, 211
MhlI GDGCHC 2 cut(s) 13, 972
MluCI AATT 6 cut(s) 359, 472, 708, 743, 762, 803
MlyI GAGTC 3 cut(s) 188, 386, 1173
MmeI TCCRAC 2 cut(s) 345, 589
Mph1103I ATGCAT 1 cut(s) 28
MroXI GAANNNNTTC 2 cut(s) 266, 312
MseI TTAA 5 cut(s) 246, 675, 738, 939, 1094
MspCI CTTAAG 1 cut(s) 1093
MspI CCGG 2 cut(s) 153, 297
MspR9I CCNGG 3 cut(s) 297, 855, 903
Mva1269I GAATGC 1 cut(s) 599
MvaI CCWGG 2 cut(s) 855, 903
MwoI GCNNNNNNNGC 8 cut(s) 107, 116, 119, 722, 976, 1130, 1193, 1253
NciI CCSGG 1 cut(s) 297
NdeI CATATG 1 cut(s) 1165
NdeII GATC 7 cut(s) 75, 211, 541, 775, 847, 1140, 1221
NlaIII CATG 3 cut(s) 526, 550, 572
NlaIV GGNNCC 3 cut(s) 174, 629, 795
NsiI ATGCAT 1 cut(s) 28
PctI GAATGC 1 cut(s) 599
PdmI GAANNNNTTC 2 cut(s) 266, 312
PfeI GAWTC 6 cut(s) 45, 149, 194, 419, 604, 1238
PfoI TCCNGGA 1 cut(s) 901
PkrI GCNGC 3 cut(s) 112, 121, 724
PleI GAGTC 3 cut(s) 187, 385, 1173
PpsI GAGTC 3 cut(s) 187, 385, 1173
PpuMI RGGWCCY 1 cut(s) 794
Psp124BI GAGCTC 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 794
Psp6I CCWGG 2 cut(s) 853, 901
PspFI CCCAGC 1 cut(s) 1124
PspGI CCWGG 2 cut(s) 853, 901
PspN4I GGNNCC 3 cut(s) 174, 629, 795
PspPI GGNCC 4 cut(s) 172, 628, 794, 863
PspPPI RGGWCCY 1 cut(s) 794
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 2 cut(s) 75, 211
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
SacI GAGCTC 1 cut(s) 13
SaqAI TTAA 5 cut(s) 246, 675, 738, 939, 1094
SatI GCNGC 3 cut(s) 111, 120, 723
Sau3AI GATC 7 cut(s) 75, 211, 541, 775, 847, 1140, 1221
Sau96I GGNCC 4 cut(s) 172, 628, 794, 863
SchI GAGTC 3 cut(s) 188, 386, 1173
ScrFI CCNGG 3 cut(s) 297, 855, 903
SduI GDGCHC 2 cut(s) 13, 972
SfaNI GCATC 7 cut(s) 35, 277, 808, 901, 928, 988, 1234
SfcI CTRYAG 1 cut(s) 317
SinI GGWCC 2 cut(s) 628, 794
SmlI CTYRAG 4 cut(s) 16, 458, 608, 1093
SmoI CTYRAG 4 cut(s) 16, 458, 608, 1093
Sse9I AATT 6 cut(s) 359, 472, 708, 743, 762, 803
SsiI CCGC 1 cut(s) 111
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 3 cut(s) 295, 853, 901
TaaI ACNGT 1 cut(s) 955
TaqI TCGA 3 cut(s) 164, 375, 429
TasI AATT 6 cut(s) 359, 472, 708, 743, 762, 803
TatI WGTACW 1 cut(s) 468
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 6 cut(s) 45, 149, 194, 419, 604, 1238
Tru1I TTAA 5 cut(s) 246, 675, 738, 939, 1094
Tru9I TTAA 5 cut(s) 246, 675, 738, 939, 1094
TscAI CASTG 1 cut(s) 960
TseI GCWGC 2 cut(s) 119, 722
TspDTI ATGAA 4 cut(s) 297, 372, 423, 1152
TspGWI ACGGA 1 cut(s) 1172
TspRI CASTG 1 cut(s) 960
Vha464I CTTAAG 1 cut(s) 1093
VpaK11BI GGWCC 2 cut(s) 628, 794
XagI CCTNNNNNAGG 1 cut(s) 858
XapI RAATTY 1 cut(s) 743
XcmI CCANNNNNNNNNTGG 1 cut(s) 873
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 2 cut(s) 266, 312
Zsp2I ATGCAT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.