MD14G1229900.v1.1

Mitochondrial ribosomal subunit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006027
Physical Location & Seq
Reverse (-)
31085623 .. 31088205
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1229900.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGAGACGAACTCTGCTGAGTAATCTCTCTCTCTGCACTCGCAATCGCCTTCTCTCTCCTCAAACATTCAACCCAAATCCTACCCCCTCACTCGTCCCACTCGCCCGTTCGACTCGGTCCCTGCTCAGCCGGTTCTTCTCTTCGGACGGCGACTCCTCCGCCGAAGGCCCCAATCCGGCTGCTGATTCGAGCTTGATCCCAACCAAAAAGAAAGATGCCTCCGTCGAAGTCAAGGACGTCAATAACAAAGAGCTGAAGGAGCGGATAGATAAATATTTTAAGGGTGATGAGGAGGCGCTCCCGGAGATACTCGAAGCAATTCTGCAGAGGAGATTGGCTGGGAAGCACGAGGAAACAGATGATGAGCTGATTGATGAATTGCAAATGCAGCCATTGGATGATGTTAAGGACCAAGAGTTTGAATCGGATTTTGAGGAATTGTACGACACGGATGAAGAGATTGATGACTTGTATAACGCAAAGGATATAGTCATGAATAGGATGTCAAAAGATGAGTACTTCAACATGGATGCCAAGAAGTGGGATGATATTGTTGAGGATGCAATTAAGCACGGGATTATGAAGGATACAAAGGAGTGTGAGGCGATTCTCGAGGATATGCTTAGCTGGGATAAGCTCCTGCCAGATGACATGAAAAGGAAGGTAGAAGAGAAGTTCAATGACTTGGGGGATAAGTGTGAAGGAGGGGAGCTTCAACCTGAAGAAGCTTATGAGTTGTTCAAGGAGTTTGAGGATGAGATTGTCATGGAGTATTTGCAGAAGATGGAAGCCGACGGCCCCCCACAATTTGATGAGGATGTGTCTGACAAGAAGGATCTGGCTGACCCACCAGGCGAAGGGCCAATCCTTAGGTGGCAAACACGGGTAGTCTTTGCTCCTGGTGGTGATTCCTGGCATCCCAAGAATAGAAAAGTGAAACTGTCTGTTACAGTGAAAGAACTCGGGCTTTCAAAGCATCAATTCCGTCGGCTCAGAGACTTGGTTGGAAAGCGGTACAATCCAGGGAAAGATGAGCTTACAATTACTAGTGAGAGGTTTGAACACCGAGAAGAAAATAGAAAGGATTGCCTTAGGACACTTCTCTCCCTCATTGAGGAAGCTGGGAAGGCTAACAAACTGGCTGAGGATGCTCGAGTTTCGTATGTCAAGGAGAGATTAAGAGCAAATCCCGCATTCATGGAGAGGCTGCACAACAAGACCATGAGATTGCAAGGAAAGAGTGCAGTACCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S28

Protein Analysis

419

Amino Acids

48.19

Weight (kDa)

4.88

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S28 PF10213 304 - 383 3.4e-17 Mitochondrial ribosomal subunit protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 240
AccB7I CCANNNNNTGG 1 cut(s) 540
AccBSI CCGCTC 1 cut(s) 262
AciI CCGC 4 cut(s) 159, 262, 1012, 1191
AclWI GGATC 2 cut(s) 190, 843
AcuI CTGAAG 2 cut(s) 275, 741
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 4 cut(s) 443, 518, 1016, 1248
AfiI CCNNNNNNNGG 4 cut(s) 175, 540, 857, 1114
AgsI TTSAA 8 cut(s) 70, 422, 523, 679, 716, 742, 972, 1061
AhlI ACTAGT 1 cut(s) 1046
AjnI CCWGG 4 cut(s) 850, 898, 911, 1021
AluBI AGCT 9 cut(s) 192, 253, 367, 627, 637, 712, 728, 1036, 1121
AluI AGCT 9 cut(s) 192, 253, 367, 627, 637, 712, 728, 1036, 1121
Alw26I GTCTC 1 cut(s) 990
AlwI GGATC 2 cut(s) 190, 843
AlwNI CAGNNNCTG 1 cut(s) 1250
Ama87I CYCGRG 3 cut(s) 611, 962, 1152
AoxI GGCC 3 cut(s) 166, 796, 860
ApeKI GCWGC 3 cut(s) 179, 388, 1207
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
Asp700I GAANNNNTTC 1 cut(s) 318
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 5 cut(s) 117, 167, 409, 797, 860
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 2 cut(s) 296, 917
AvaI CYCGRG 3 cut(s) 611, 962, 1152
AvaII GGWCC 2 cut(s) 117, 409
AxyI CCTNAGG 2 cut(s) 869, 1091
BauI CACGAG 1 cut(s) 347
BbvCI CCTCAGC 1 cut(s) 1143
BbvI GCAGC 3 cut(s) 166, 400, 1194
BccI CCATC 1 cut(s) 778
BceAI ACGGC 2 cut(s) 163, 811
BciT130I CCWGG 4 cut(s) 852, 900, 913, 1023
BciVI GTATCC 1 cut(s) 580
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 990
BcuI ACTAGT 1 cut(s) 1046
BfaI CTAG 1 cut(s) 1047
BfmI CTRYAG 1 cut(s) 323
BfoI RGCGCY 1 cut(s) 299
BfuI GTATCC 1 cut(s) 580
BisI GCNGC 3 cut(s) 180, 389, 1208
BlpI GCTNAGC 2 cut(s) 125, 623
BlsI GCNGC 3 cut(s) 181, 390, 1209
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 5 cut(s) 302, 852, 900, 913, 1023
Bme18I GGWCC 2 cut(s) 117, 409
BmeT110I CYCGRG 3 cut(s) 611, 962, 1152
BmgT120I GGNCC 5 cut(s) 117, 167, 409, 797, 860
BmiI GGNNCC 3 cut(s) 119, 169, 799
BmrFI CCNGG 5 cut(s) 302, 852, 900, 913, 1023
BmsI GCATC 6 cut(s) 205, 520, 550, 925, 985, 1138
BplI GAGNNNNNCTC 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 1143
Bpu1102I GCTNAGC 2 cut(s) 125, 623
BpuMI CCSGG 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 1022
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 540, 857, 1114
Bse118I RCCGGY 1 cut(s) 129
Bse1I ACTGG 1 cut(s) 1143
Bse21I CCTNAGG 2 cut(s) 869, 1091
BseBI CCWGG 4 cut(s) 852, 900, 913, 1023
BseDI CCNNGG 1 cut(s) 1022
BseLI CCNNNNNNNGG 4 cut(s) 175, 540, 857, 1114
BseMII CTCAG 4 cut(s) 8, 139, 1006, 1134
BseNI ACTGG 1 cut(s) 1143
BseRI GAGGAG 4 cut(s) 48, 145, 305, 343
BseXI GCAGC 3 cut(s) 166, 400, 1194
BseYI CCCAGC 3 cut(s) 338, 627, 1121
BsgI GTGCAG 2 cut(s) 19, 1193
BshFI GGCC 3 cut(s) 168, 798, 862
BsiHKCI CYCGRG 3 cut(s) 611, 962, 1152
BsiSI CCGG 3 cut(s) 130, 176, 302
BslFI GGGAC 2 cut(s) 80, 103
BslI CCNNNNNNNGG 4 cut(s) 175, 540, 857, 1114
BsmAI GTCTC 1 cut(s) 990
BsmFI GGGAC 2 cut(s) 80, 103
BsmI GAATGC 1 cut(s) 1193
BsnI GGCC 3 cut(s) 168, 798, 862
BsoBI CYCGRG 3 cut(s) 611, 962, 1152
Bsp143I GATC 2 cut(s) 195, 835
Bsp1720I GCTNAGC 2 cut(s) 125, 623
BspACI CCGC 4 cut(s) 159, 262, 1012, 1191
BspANI GGCC 3 cut(s) 168, 798, 862
BspCNI CTCAG 4 cut(s) 9, 138, 1005, 1135
BspHI TCATGA 1 cut(s) 492
BspLI GGNNCC 3 cut(s) 119, 169, 799
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 2 cut(s) 190, 843
BsrBI CCGCTC 1 cut(s) 262
BsrFI RCCGGY 1 cut(s) 129
BsrI ACTGG 1 cut(s) 1143
BssAI RCCGGY 1 cut(s) 129
BssECI CCNNGG 1 cut(s) 1022
BssMI GATC 2 cut(s) 195, 835
BssNI GRCGYC 1 cut(s) 237
BssSI CACGAG 1 cut(s) 347
Bst2BI CACGAG 1 cut(s) 347
Bst2UI CCWGG 4 cut(s) 852, 900, 913, 1023
Bst4CI ACNGT 2 cut(s) 942, 952
Bst6I CTCTTC 3 cut(s) 145, 450, 663
BstACI GRCGYC 1 cut(s) 237
BstAPI GCANNNNNTGC 1 cut(s) 1250
BstDEI CTNAG 7 cut(s) 17, 125, 623, 869, 992, 1091, 1143
BstENI CCTNNNNNAGG 1 cut(s) 1112
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 2 cut(s) 198, 838
BstMAI GTCTC 1 cut(s) 990
BstMBI GATC 2 cut(s) 195, 835
BstMWI GCNNNNNNNGC 7 cut(s) 259, 388, 973, 1127, 1148, 1190, 1250
BstNI CCWGG 4 cut(s) 852, 900, 913, 1023
BstSCI CCNGG 5 cut(s) 300, 850, 898, 911, 1021
BstSFI CTRYAG 1 cut(s) 323
BstV1I GCAGC 3 cut(s) 166, 400, 1194
BstX2I RGATCY 1 cut(s) 835
BstYI RGATCY 1 cut(s) 835
Bsu36I CCTNAGG 2 cut(s) 869, 1091
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 3 cut(s) 168, 798, 862
BtsIMutI CAGTG 1 cut(s) 957
CaiI CAGNNNCTG 1 cut(s) 1250
CciI TCATGA 1 cut(s) 492
CfoI GCGC 1 cut(s) 298
Cfr10I RCCGGY 1 cut(s) 129
Cfr13I GGNCC 5 cut(s) 117, 167, 409, 797, 860
Csp6I GTAC 4 cut(s) 442, 517, 1015, 1247
CviAII CATG 6 cut(s) 493, 526, 652, 766, 1198, 1222
CviQI GTAC 4 cut(s) 442, 517, 1015, 1247
DdeI CTNAG 7 cut(s) 17, 125, 623, 869, 992, 1091, 1143
DpnI GATC 2 cut(s) 197, 837
DpnII GATC 2 cut(s) 195, 835
Eam1104I CTCTTC 3 cut(s) 145, 450, 663
EarI CTCTTC 3 cut(s) 145, 450, 663
EciI GGCGGA 1 cut(s) 148
Eco47I GGWCC 2 cut(s) 117, 409
Eco57I CTGAAG 2 cut(s) 275, 741
Eco81I CCTNAGG 2 cut(s) 869, 1091
Eco88I CYCGRG 3 cut(s) 611, 962, 1152
EcoNI CCTNNNNNAGG 1 cut(s) 1112
EcoO109I RGGNCCY 1 cut(s) 167
EcoRII CCWGG 4 cut(s) 850, 898, 911, 1021
FaeI CATG 6 cut(s) 496, 529, 655, 769, 1201, 1225
FalI AAGNNNNNCTT 2 cut(s) 1020, 1052
FaqI GGGAC 2 cut(s) 80, 103
FatI CATG 6 cut(s) 492, 525, 651, 765, 1197, 1221
FauI CCCGC 1 cut(s) 1198
Fnu4HI GCNGC 3 cut(s) 180, 389, 1208
Fsp4HI GCNGC 3 cut(s) 180, 389, 1208
FspBI CTAG 1 cut(s) 1047
GlaI GCGC 1 cut(s) 297
GluI GCNGC 3 cut(s) 180, 389, 1208
GsaI CCCAGC 3 cut(s) 342, 631, 1125
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 3 cut(s) 168, 798, 862
HapII CCGG 3 cut(s) 130, 176, 302
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 6 cut(s) 496, 529, 655, 769, 1201, 1225
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HindIII AAGCTT 1 cut(s) 726
HinfI GANTC 6 cut(s) 112, 152, 185, 422, 607, 908
HpaII CCGG 3 cut(s) 130, 176, 302
HphI GGTGA 2 cut(s) 296, 917
Hpy188I TCNGA 4 cut(s) 145, 427, 826, 995
Hpy188III TCNNGA 2 cut(s) 493, 611
Hpy99I CGWCG 3 cut(s) 227, 797, 990
HpyAV CCTTC 9 cut(s) 59, 158, 250, 577, 655, 695, 826, 851, 1120
HpyCH4III ACNGT 2 cut(s) 942, 952
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 9 cut(s) 36, 325, 382, 388, 563, 778, 1210, 1231, 1244
HpyF10VI GCNNNNNNNGC 7 cut(s) 259, 388, 973, 1127, 1148, 1190, 1250
HpyF3I CTNAG 7 cut(s) 17, 125, 623, 869, 992, 1091, 1143
HpySE526I ACGT 1 cut(s) 237
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 6 cut(s) 496, 529, 655, 769, 1201, 1225
HspAI GCGC 1 cut(s) 296
Kzo9I GATC 2 cut(s) 195, 835
LmnI GCTCC 5 cut(s) 259, 303, 642, 709, 901
Lsp1109I GCAGC 3 cut(s) 166, 400, 1194
LweI GCATC 6 cut(s) 205, 520, 550, 925, 985, 1138
MaeI CTAG 1 cut(s) 1047
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 946
MalI GATC 2 cut(s) 197, 837
MbiI CCGCTC 1 cut(s) 262
MboI GATC 2 cut(s) 195, 835
MboII GAAGA 7 cut(s) 127, 132, 467, 680, 734, 793, 1082
MflI RGATCY 1 cut(s) 835
MluCI AATT 7 cut(s) 318, 377, 437, 564, 806, 980, 1041
MlyI GAGTC 2 cut(s) 106, 146
MmeI TCCRAC 1 cut(s) 985
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 4 cut(s) 279, 405, 567, 1178
MspI CCGG 3 cut(s) 130, 176, 302
MspR9I CCNGG 5 cut(s) 302, 852, 900, 913, 1023
Mva1269I GAATGC 1 cut(s) 1193
MvaI CCWGG 4 cut(s) 852, 900, 913, 1023
MwoI GCNNNNNNNGC 7 cut(s) 259, 388, 973, 1127, 1148, 1190, 1250
NciI CCSGG 1 cut(s) 302
NdeII GATC 2 cut(s) 195, 835
NlaIII CATG 6 cut(s) 496, 529, 655, 769, 1201, 1225
NlaIV GGNNCC 3 cut(s) 119, 169, 799
PaeR7I CTCGAG 2 cut(s) 611, 1152
PagI TCATGA 1 cut(s) 492
PctI GAATGC 1 cut(s) 1193
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 4 cut(s) 185, 422, 607, 908
PflFI GACNNNGTC 1 cut(s) 115
PflMI CCANNNNNTGG 1 cut(s) 540
PfoI TCCNGGA 1 cut(s) 300
PkrI GCNGC 3 cut(s) 181, 390, 1209
PleI GAGTC 2 cut(s) 106, 146
PpsI GAGTC 2 cut(s) 106, 146
Psp6I CCWGG 4 cut(s) 850, 898, 911, 1021
PspFI CCCAGC 3 cut(s) 338, 627, 1121
PspGI CCWGG 4 cut(s) 850, 898, 911, 1021
PspN4I GGNNCC 3 cut(s) 119, 169, 799
PspPI GGNCC 5 cut(s) 117, 167, 409, 797, 860
PspXI VCTCGAGB 1 cut(s) 1152
PstI CTGCAG 1 cut(s) 327
PstNI CAGNNNCTG 1 cut(s) 1250
PsuI RGATCY 1 cut(s) 835
PsyI GACNNNGTC 1 cut(s) 115
RsaI GTAC 4 cut(s) 443, 518, 1016, 1248
RsaNI GTAC 4 cut(s) 442, 517, 1015, 1247
SaqAI TTAA 4 cut(s) 279, 405, 567, 1178
SatI GCNGC 3 cut(s) 180, 389, 1208
Sau3AI GATC 2 cut(s) 195, 835
Sau96I GGNCC 5 cut(s) 117, 167, 409, 797, 860
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 2 cut(s) 106, 146
ScrFI CCNGG 5 cut(s) 302, 852, 900, 913, 1023
SfaNI GCATC 6 cut(s) 205, 520, 550, 925, 985, 1138
SfcI CTRYAG 1 cut(s) 323
Sfr274I CTCGAG 2 cut(s) 611, 1152
SinI GGWCC 2 cut(s) 117, 409
SlaI CTCGAG 2 cut(s) 611, 1152
SmlI CTYRAG 2 cut(s) 611, 1152
SmoI CTYRAG 2 cut(s) 611, 1152
SpeI ACTAGT 1 cut(s) 1046
Sse9I AATT 7 cut(s) 318, 377, 437, 564, 806, 980, 1041
SsiI CCGC 4 cut(s) 159, 262, 1012, 1191
SspI AATATT 1 cut(s) 275
SspMI CTAG 1 cut(s) 1047
StyD4I CCNGG 5 cut(s) 300, 850, 898, 911, 1021
TaaI ACNGT 2 cut(s) 942, 952
TaiI ACGT 1 cut(s) 240
TaqI TCGA 6 cut(s) 110, 188, 225, 312, 612, 1153
TaqII GACCGA 1 cut(s) 105
TasI AATT 7 cut(s) 318, 377, 437, 564, 806, 980, 1041
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 4 cut(s) 185, 422, 607, 908
Tru1I TTAA 4 cut(s) 279, 405, 567, 1178
Tru9I TTAA 4 cut(s) 279, 405, 567, 1178
TscAI CASTG 1 cut(s) 957
TseI GCWGC 3 cut(s) 179, 388, 1207
TspDTI ATGAA 6 cut(s) 390, 468, 509, 596, 668, 1186
TspGWI ACGGA 3 cut(s) 211, 464, 974
TspRI CASTG 1 cut(s) 957
Tth111I GACNNNGTC 1 cut(s) 115
Van91I CCANNNNNTGG 1 cut(s) 540
VpaK11BI GGWCC 2 cut(s) 117, 409
XagI CCTNNNNNAGG 1 cut(s) 1112
XcmI CCANNNNNNNNNTGG 1 cut(s) 870
XhoI CTCGAG 2 cut(s) 611, 1152
XmnI GAANNNNTTC 1 cut(s) 318
XspI CTAG 1 cut(s) 1047
ZraI GACGTC 1 cut(s) 238
ZrmI AGTACT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.