Rorug02G0278300

Mitochondrial ribosomal subunit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006027
Physical Location & Seq
Forward (+)
30014119 .. 30014739
621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0278300.1

Sequence Viewer

Length: 621 bp
ATGGATCAATGGCTTCTTAGGCAAACAAGACATCTTCCCAGTAAGAGTGAGAAAGAAAGGATTCTTACCTTTATTAGCTTCTTGGTTTGGAACATCTGGAAGGCGAGGTGTGCTTTTGTTTATGAGCATAAGCCCCTCTCTCCAAGCTATACCATCAGTACCACCATCAAGCTAGTAACTGAGTTTCTTGATGCAACAAACCATTCCCTGGTCAATCTGATCCATGACACTTATGAATCGGAACCTCATCCAGCTTGGAGTGCTCCCCCGGTTGAGCATGTCAAAGTCAATTGTGATGCGTCTTGGCTAGCCCCAAATTCAGCCGGACTCGGAGTAGTCATCAGGGATCATACTGGCTATCTCCTTGGGGGTTCTGCTACCCAGGCCATATGCTCTTCAGTGGAAATTGCAGAATCTGAAGCATTACTATCTGGGGTAAATCTTGCAGCATCATTGAATTTGAAAAAGGTAAAATTTGAATCAGATGCAAGGGAGGTCATCACTAACCTGAAGAATCCAAGGGCTCGGAGCTGGAGATTGTTTCTGATCATCGATCAAATCCGGAAACGTTGCTCGATGTTTGATTACTGCACTTGGGAGTTGGGACTGGGTTCCCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S28

Protein Analysis

206

Amino Acids

23.25

Weight (kDa)

7.69

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 97 - 201 1.7e-18 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 208
AccIII TCCGGA 1 cut(s) 561
AclI AACGTT 1 cut(s) 568
AclWI GGATC 3 cut(s) 12, 214, 354
AcsI RAATTY 3 cut(s) 316, 457, 473
AcuI CTGAAG 3 cut(s) 381, 438, 530
AfaI GTAC 1 cut(s) 160
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 3 cut(s) 457, 463, 479
AjnI CCWGG 2 cut(s) 207, 381
AluBI AGCT 5 cut(s) 78, 147, 172, 254, 531
AluI AGCT 5 cut(s) 78, 147, 172, 254, 531
Alw21I GWGCWC 1 cut(s) 265
AlwI GGATC 3 cut(s) 12, 214, 354
AlwNI CAGNNNCTG 1 cut(s) 416
Aor13HI TCCGGA 1 cut(s) 561
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 3 cut(s) 316, 457, 473
Asp700I GAANNNNTTC 1 cut(s) 60
AsuC2I CCSGG 1 cut(s) 269
AsuNHI GCTAGC 1 cut(s) 307
BanII GRGCYC 1 cut(s) 526
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 1 cut(s) 458
BccI CCATC 2 cut(s) 161, 173
BciT130I CCWGG 2 cut(s) 209, 383
BclI TGATCA 1 cut(s) 546
BcnI CCSGG 1 cut(s) 269
BfaI CTAG 2 cut(s) 173, 308
BisI GCNGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 448
Bme1390I CCNGG 3 cut(s) 209, 269, 383
BmiI GGNNCC 2 cut(s) 243, 613
BmrFI CCNGG 3 cut(s) 209, 269, 383
BmrI ACTGGG 2 cut(s) 33, 617
BmsI GCATC 4 cut(s) 181, 286, 458, 475
BmtI GCTAGC 1 cut(s) 311
BmuI ACTGGG 2 cut(s) 33, 617
BpmI CTGGAG 1 cut(s) 553
BpuMI CCSGG 1 cut(s) 269
Bsa29I ATCGAT 1 cut(s) 552
BsaJI CCNNGG 5 cut(s) 207, 267, 364, 381, 518
BsaWI WCCGGW 1 cut(s) 561
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse1I ACTGG 3 cut(s) 39, 358, 612
BseAI TCCGGA 1 cut(s) 561
BseBI CCWGG 2 cut(s) 209, 383
BseCI ATCGAT 1 cut(s) 552
BseDI CCNNGG 5 cut(s) 207, 267, 364, 381, 518
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 3 cut(s) 39, 358, 612
BseXI GCAGC 1 cut(s) 458
BsgI GTGCAG 1 cut(s) 574
BshFI GGCC 1 cut(s) 386
BshVI ATCGAT 1 cut(s) 552
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 3 cut(s) 269, 324, 562
BslI CCNNNNNNNGG 1 cut(s) 208
BsnI GGCC 1 cut(s) 386
Bsp1286I GDGCHC 2 cut(s) 265, 526
Bsp13I TCCGGA 1 cut(s) 561
Bsp143I GATC 5 cut(s) 4, 219, 346, 546, 553
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 1 cut(s) 172
BspDI ATCGAT 1 cut(s) 552
BspEI TCCGGA 1 cut(s) 561
BspLI GGNNCC 2 cut(s) 243, 613
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 3 cut(s) 12, 214, 354
BspQI GCTCTTC 1 cut(s) 400
BsrI ACTGG 3 cut(s) 39, 358, 612
BssECI CCNNGG 5 cut(s) 207, 267, 364, 381, 518
BssMI GATC 5 cut(s) 4, 219, 346, 546, 553
BssT1I CCWWGG 2 cut(s) 364, 518
Bst2UI CCWGG 2 cut(s) 209, 383
Bst6I CTCTTC 1 cut(s) 400
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 2 cut(s) 17, 180
BstF5I GGATG 1 cut(s) 247
BstKTI GATC 5 cut(s) 7, 222, 349, 549, 556
BstMBI GATC 5 cut(s) 4, 219, 346, 546, 553
BstMWI GCNNNNNNNGC 4 cut(s) 19, 110, 260, 383
BstNI CCWGG 2 cut(s) 209, 383
BstNSI RCATGY 1 cut(s) 281
BstSCI CCNGG 3 cut(s) 207, 267, 381
BstV1I GCAGC 1 cut(s) 458
Bsu15I ATCGAT 1 cut(s) 552
BsuRI GGCC 1 cut(s) 386
BsuTUI ATCGAT 1 cut(s) 552
BtsCI GGATG 1 cut(s) 247
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 1 cut(s) 309
CaiI CAGNNNCTG 1 cut(s) 416
ClaI ATCGAT 1 cut(s) 552
CseI GACGC 1 cut(s) 288
Csp6I GTAC 1 cut(s) 159
CviAII CATG 2 cut(s) 224, 278
CviQI GTAC 1 cut(s) 159
DdeI CTNAG 2 cut(s) 17, 180
DpnI GATC 5 cut(s) 6, 221, 348, 548, 555
DpnII GATC 5 cut(s) 4, 219, 346, 546, 553
Eam1104I CTCTTC 1 cut(s) 400
EarI CTCTTC 1 cut(s) 400
Eco130I CCWWGG 2 cut(s) 364, 518
Eco24I GRGCYC 1 cut(s) 526
Eco57I CTGAAG 3 cut(s) 381, 438, 530
EcoRII CCWGG 2 cut(s) 207, 381
EcoT14I CCWWGG 2 cut(s) 364, 518
EcoT38I GRGCYC 1 cut(s) 526
ErhI CCWWGG 2 cut(s) 364, 518
FaeI CATG 2 cut(s) 227, 281
FaiI YATR 9 cut(s) 123, 129, 150, 225, 234, 279, 351, 389, 391
FatI CATG 2 cut(s) 223, 277
FauNDI CATATG 1 cut(s) 389
FbaI TGATCA 1 cut(s) 546
Fnu4HI GCNGC 1 cut(s) 447
FokI GGATG 1 cut(s) 234
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 1 cut(s) 447
FspBI CTAG 2 cut(s) 173, 308
GluI GCNGC 1 cut(s) 447
GsuI CTGGAG 1 cut(s) 553
HaeIII GGCC 1 cut(s) 386
HapII CCGG 3 cut(s) 269, 324, 562
HgaI GACGC 1 cut(s) 288
Hin1II CATG 2 cut(s) 227, 281
HinfI GANTC 6 cut(s) 61, 236, 327, 413, 479, 514
HpaII CCGG 3 cut(s) 269, 324, 562
Hpy188I TCNGA 7 cut(s) 219, 241, 332, 418, 484, 528, 546
Hpy188III TCNNGA 3 cut(s) 97, 188, 562
HpyAV CCTTC 1 cut(s) 94
HpyCH4IV ACGT 1 cut(s) 568
HpyCH4V TGCA 5 cut(s) 194, 410, 446, 488, 591
HpyF10VI GCNNNNNNNGC 4 cut(s) 19, 110, 260, 383
HpyF3I CTNAG 2 cut(s) 17, 180
HpySE526I ACGT 1 cut(s) 568
Hsp92II CATG 2 cut(s) 227, 281
Kpn2I TCCGGA 1 cut(s) 561
Ksp22I TGATCA 1 cut(s) 546
Kzo9I GATC 5 cut(s) 4, 219, 346, 546, 553
LguI GCTCTTC 1 cut(s) 400
LmnI GCTCC 2 cut(s) 268, 528
Lsp1109I GCAGC 1 cut(s) 458
LweI GCATC 4 cut(s) 181, 286, 458, 475
MaeI CTAG 2 cut(s) 173, 308
MaeII ACGT 1 cut(s) 568
MaeIII GTNAC 1 cut(s) 175
MalI GATC 5 cut(s) 6, 221, 348, 548, 555
MboI GATC 5 cut(s) 4, 219, 346, 546, 553
MboII GAAGA 3 cut(s) 26, 387, 523
MfeI CAATTG 1 cut(s) 289
MhlI GDGCHC 2 cut(s) 265, 526
MluCI AATT 5 cut(s) 289, 316, 405, 457, 473
MlyI GAGTC 1 cut(s) 321
MnlI CCTC 4 cut(s) 99, 146, 255, 487
MroI TCCGGA 1 cut(s) 561
MroXI GAANNNNTTC 1 cut(s) 60
MspI CCGG 3 cut(s) 269, 324, 562
MspR9I CCNGG 3 cut(s) 209, 269, 383
MunI CAATTG 1 cut(s) 289
MvaI CCWGG 2 cut(s) 209, 383
MwoI GCNNNNNNNGC 4 cut(s) 19, 110, 260, 383
NciI CCSGG 1 cut(s) 269
NdeI CATATG 1 cut(s) 389
NdeII GATC 5 cut(s) 4, 219, 346, 546, 553
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 2 cut(s) 227, 281
NlaIV GGNNCC 2 cut(s) 243, 613
NspI RCATGY 1 cut(s) 281
PciSI GCTCTTC 1 cut(s) 400
PdmI GAANNNNTTC 1 cut(s) 60
PfeI GAWTC 5 cut(s) 61, 236, 413, 479, 514
PflMI CCANNNNNTGG 1 cut(s) 208
PkrI GCNGC 1 cut(s) 448
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
Psp1406I AACGTT 1 cut(s) 568
Psp6I CCWGG 2 cut(s) 207, 381
PspGI CCWGG 2 cut(s) 207, 381
PspN4I GGNNCC 2 cut(s) 243, 613
PstNI CAGNNNCTG 1 cut(s) 416
RsaI GTAC 1 cut(s) 160
RsaNI GTAC 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 400
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 5 cut(s) 4, 219, 346, 546, 553
SchI GAGTC 1 cut(s) 321
ScrFI CCNGG 3 cut(s) 209, 269, 383
SduI GDGCHC 2 cut(s) 265, 526
SfaNI GCATC 4 cut(s) 181, 286, 458, 475
Sse9I AATT 5 cut(s) 289, 316, 405, 457, 473
SspMI CTAG 2 cut(s) 173, 308
StyD4I CCNGG 3 cut(s) 207, 267, 381
StyI CCWWGG 2 cut(s) 364, 518
TaiI ACGT 1 cut(s) 571
TaqI TCGA 2 cut(s) 552, 575
TasI AATT 5 cut(s) 289, 316, 405, 457, 473
TfiI GAWTC 5 cut(s) 61, 236, 413, 479, 514
TscAI CASTG 1 cut(s) 405
TseI GCWGC 1 cut(s) 446
TspDTI ATGAA 1 cut(s) 249
TspRI CASTG 1 cut(s) 405
Van91I CCANNNNNTGG 1 cut(s) 208
XapI RAATTY 3 cut(s) 316, 457, 473
XceI RCATGY 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 60
XspI CTAG 2 cut(s) 173, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.