AT4G21460

Mitochondrial ribosomal subunit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006027
Physical Location & Seq
Forward (+)
11428445 .. 11430768
2324 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21460.2

Sequence Viewer

Length: 1248 bp
ATGAGAGGAGCTCTGTTCAGAAATGCATCTCTCTGCGCTCGCAGAATCATTCTTTCTTCTAGGATCTCCCCCAATGTTCCCTTTCTCACTCCAATCGCCGCTCCGGCTCCTCCCAAATTCAGATTTTTCAGTTCCGAATCCGGTGAGAACTCGACTACTGCCACTGAGTCTTCGCCGACTGATTCATCCGATAAGAAGGATCTCGTTGTAGAGGATGTCAGCAACAAAGAGCTTAAAAGCCGTATAGACAAGTCTTTCAATGAGGGTAATGAAGATGCTTTACCTGGAGTTATTGAAGCACTTCTACAGAGAAGACTTGTAGACAAACACGCAGAGACTGATGATGAATTGATGGAAAAAATCGAGAGTCTACCTTTCAAAGATGATGTCAAGGATGAAGATTTTGAATCCGATTTCGAAGAAGCGCATTCTACTGACGAAGAACTTGAGGATTTGTACAACTCTCCCGAGTATGTAGCTGAAAAGATGAGGAAGAAAGAATTTTTCAACATGGACGATAACAAGTGGGATCACATGATTAGAGAAGGTATCCAACATGGCTGTCTTACCGATACTAAACAATGCGAGGAGATTCTCGAGGATATGCTCAAATGGGACCAGCTTCTTCCTGATGACTTGAAGAAAAAAGTTGAAGCAAAGTTTAATGAGCTCGGGGATATGTGTGAAAGAGGTGAAATTGAGGCCGAAGCAGCTTATGAGCTTTTCAAGGAATTTGAAGATGAGATGGTAATTCAGTATGGGGATCAGATGGAAGCTGAGGGTCCTCCCCAATTTGGTGAAACAGATACTTCTGACAGGAATACTGACTTGGATGATCCCTCCGGTAAAGGCCCAATCCTCAGGTGGCAATCAAGGATAGTTTTTGCTCCTGGAGGTGATGCGTGGCATCCAAAGAACAGGAAAGTTAAAATGTCTGTTACAGTGAAAGAGCTTGGGCTCTCAAATCATCAGGCTAAAAGGCTAAGGGAACTTGTGGGAAAAAGATACCATTCAGGGAAAGATGAGCTTACAATCACCTGCGAGAGGTTTGAACACCGAGAAGAAAACAGGAAAGACTGCTTGAGAACTCTTTACGGGTTGATAGAGGAAGCTGGGAAAGCCAACAAGATCGCTGAAGACATAAGAACTTCATATGTAAAACAGAGACTCCGAGCCAGTCCAGCTTTTATGCAGAAACTACAAGCCAAGATCATAAGGTCTAAAGAATCAAATGCCATCAACGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S28

Protein Analysis

415

Amino Acids

47.55

Weight (kDa)

4.96

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S28 PF10213 314 - 394 1.6e-16 Mitochondrial ribosomal subunit protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1046
Acc36I ACCTGC 1 cut(s) 1046
AccBSI CCGCTC 1 cut(s) 101
AccI GTMKAC 2 cut(s) 321, 370
AciI CCGC 1 cut(s) 99
AclWI GGATC 5 cut(s) 71, 207, 537, 771, 830
AcsI RAATTY 3 cut(s) 116, 500, 731
AcuI CTGAAG 1 cut(s) 1155
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 2 cut(s) 794, 1044
AjnI CCWGG 2 cut(s) 283, 889
AjuI GAANNNNNNNTTGG 2 cut(s) 812, 844
Alw21I GWGCWC 2 cut(s) 13, 672
Alw26I GTCTC 2 cut(s) 329, 1159
AlwI GGATC 5 cut(s) 71, 207, 537, 771, 830
AlwNI CAGNNNCTG 1 cut(s) 338
Ama87I CYCGRG 3 cut(s) 467, 596, 671
AoxI GGCC 2 cut(s) 702, 850
ApeKI GCWGC 1 cut(s) 710
ApoI RAATTY 3 cut(s) 116, 500, 731
ArsI GACNNNNNNTTYG 2 cut(s) 372, 404
Asp700I GAANNNNTTC 1 cut(s) 300
AspLEI GCGC 2 cut(s) 38, 427
AspS9I GGNCC 3 cut(s) 616, 782, 851
AsuHPI GGTGA 5 cut(s) 155, 704, 809, 908, 1027
AsuII TTCGAA 1 cut(s) 417
AvaI CYCGRG 3 cut(s) 467, 596, 671
AvaII GGWCC 2 cut(s) 616, 782
AxyI CCTNAGG 1 cut(s) 860
BanII GRGCYC 3 cut(s) 13, 672, 960
BarI GAAGNNNNNNTAC 4 cut(s) 264, 288, 296, 320
BbsI GAAGAC 3 cut(s) 162, 319, 1143
Bbv12I GWGCWC 2 cut(s) 13, 672
BbvCI CCTCAGC 1 cut(s) 777
BbvI GCAGC 1 cut(s) 722
BccI CCATC 4 cut(s) 346, 739, 763, 1244
BceAI ACGGC 1 cut(s) 225
BciT130I CCWGG 2 cut(s) 285, 891
BciVI GTATCC 1 cut(s) 560
BcoDI GTCTC 2 cut(s) 329, 1159
BfaI CTAG 1 cut(s) 60
BfmI CTRYAG 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 1046
BfuI GTATCC 1 cut(s) 560
BglI GCCNNNNNGGC 1 cut(s) 104
BisI GCNGC 2 cut(s) 99, 711
BlsI GCNGC 2 cut(s) 100, 712
Bme1390I CCNGG 2 cut(s) 285, 891
Bme18I GGWCC 2 cut(s) 616, 782
BmeT110I CYCGRG 3 cut(s) 467, 596, 671
BmgT120I GGNCC 3 cut(s) 616, 782, 851
BmiI GGNNCC 3 cut(s) 108, 617, 783
BmrFI CCNGG 2 cut(s) 285, 891
BmsI GCATC 4 cut(s) 35, 265, 889, 916
BpiI GAAGAC 3 cut(s) 162, 319, 1143
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 2 cut(s) 306, 912
Bpu10I CCTNAGC 2 cut(s) 777, 983
Bpu14I TTCGAA 1 cut(s) 417
BpuEI CTTGAG 2 cut(s) 467, 1102
BsaWI WCCGGW 2 cut(s) 140, 842
BsaXI ACNNNNNCTCC 2 cut(s) 1152, 1182
Bsc4I CCNNNNNNNGG 2 cut(s) 794, 1044
Bse1I ACTGG 1 cut(s) 1176
Bse21I CCTNAGG 1 cut(s) 860
BseBI CCWGG 2 cut(s) 285, 891
BseGI GGATG 5 cut(s) 185, 220, 400, 838, 907
BseLI CCNNNNNNNGG 2 cut(s) 794, 1044
BseMII CTCAG 3 cut(s) 156, 768, 874
BseNI ACTGG 1 cut(s) 1176
BseRI GAGGAG 3 cut(s) 21, 99, 602
BseXI GCAGC 1 cut(s) 722
BseYI CCCAGC 1 cut(s) 1112
BshFI GGCC 2 cut(s) 704, 852
BsiHKAI GWGCWC 2 cut(s) 13, 672
BsiHKCI CYCGRG 3 cut(s) 467, 596, 671
BsiSI CCGG 3 cut(s) 104, 141, 843
BslFI GGGAC 1 cut(s) 629
BslI CCNNNNNNNGG 2 cut(s) 794, 1044
BsmAI GTCTC 2 cut(s) 329, 1159
BsmFI GGGAC 1 cut(s) 629
BsmI GAATGC 1 cut(s) 427
BsnI GGCC 2 cut(s) 704, 852
BsoBI CYCGRG 3 cut(s) 467, 596, 671
Bsp119I TTCGAA 1 cut(s) 417
Bsp1286I GDGCHC 3 cut(s) 13, 672, 960
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 7 cut(s) 63, 199, 529, 763, 835, 1128, 1209
BspACI CCGC 1 cut(s) 99
BspANI GGCC 2 cut(s) 704, 852
BspCNI CTCAG 3 cut(s) 157, 769, 873
BspLI GGNNCC 3 cut(s) 108, 617, 783
BspMI ACCTGC 1 cut(s) 1046
BspPI GGATC 5 cut(s) 71, 207, 537, 771, 830
BspT104I TTCGAA 1 cut(s) 417
BsrBI CCGCTC 1 cut(s) 101
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 1 cut(s) 1176
BssMI GATC 7 cut(s) 63, 199, 529, 763, 835, 1128, 1209
Bst2UI CCWGG 2 cut(s) 285, 891
Bst4CI ACNGT 1 cut(s) 943
BstAUI TGTACA 1 cut(s) 456
BstBI TTCGAA 1 cut(s) 417
BstC8I GCNNGC 1 cut(s) 40
BstDEI CTNAG 4 cut(s) 165, 777, 860, 983
BstENI CCTNNNNNAGG 1 cut(s) 1042
BstF5I GGATG 5 cut(s) 185, 220, 400, 838, 907
BstHHI GCGC 2 cut(s) 38, 427
BstKTI GATC 7 cut(s) 66, 202, 532, 766, 838, 1131, 1212
BstMAI GTCTC 2 cut(s) 329, 1159
BstMBI GATC 7 cut(s) 63, 199, 529, 763, 835, 1128, 1209
BstMWI GCNNNNNNNGC 5 cut(s) 104, 710, 1118, 1181, 1241
BstNI CCWGG 2 cut(s) 285, 891
BstSCI CCNGG 2 cut(s) 283, 889
BstSFI CTRYAG 1 cut(s) 305
BstV1I GCAGC 1 cut(s) 722
BstV2I GAAGAC 3 cut(s) 162, 319, 1143
BstX2I RGATCY 2 cut(s) 63, 199
BstYI RGATCY 2 cut(s) 63, 199
Bsu36I CCTNAGG 1 cut(s) 860
BsuI GTATCC 1 cut(s) 560
BsuRI GGCC 2 cut(s) 704, 852
BtsCI GGATG 5 cut(s) 185, 220, 400, 838, 907
BtsIMutI CAGTG 2 cut(s) 162, 948
BveI ACCTGC 1 cut(s) 1046
Cac8I GCNNGC 1 cut(s) 40
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 2 cut(s) 38, 427
Cfr13I GGNCC 3 cut(s) 616, 782, 851
Csp6I GTAC 1 cut(s) 457
CviAII CATG 3 cut(s) 511, 535, 557
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 4 cut(s) 165, 777, 860, 983
DpnI GATC 7 cut(s) 65, 201, 531, 765, 837, 1130, 1211
DpnII GATC 7 cut(s) 63, 199, 529, 763, 835, 1128, 1209
Ecl136II GAGCTC 2 cut(s) 11, 670
Eco24I GRGCYC 3 cut(s) 13, 672, 960
Eco47I GGWCC 2 cut(s) 616, 782
Eco53kI GAGCTC 2 cut(s) 11, 670
Eco57I CTGAAG 1 cut(s) 1155
Eco81I CCTNAGG 1 cut(s) 860
Eco88I CYCGRG 3 cut(s) 467, 596, 671
EcoICRI GAGCTC 2 cut(s) 11, 670
EcoNI CCTNNNNNAGG 1 cut(s) 1042
EcoO109I RGGNCCY 1 cut(s) 782
EcoRII CCWGG 2 cut(s) 283, 889
EcoT22I ATGCAT 1 cut(s) 28
EcoT38I GRGCYC 3 cut(s) 13, 672, 960
FaeI CATG 3 cut(s) 514, 538, 560
FalI AAGNNNNNCTT 2 cut(s) 1011, 1043
FaqI GGGAC 1 cut(s) 629
FatI CATG 3 cut(s) 510, 534, 556
FauNDI CATATG 1 cut(s) 1153
FblI GTMKAC 2 cut(s) 321, 370
Fnu4HI GCNGC 2 cut(s) 99, 711
FokI GGATG 5 cut(s) 172, 227, 407, 845, 894
FriOI GRGCYC 3 cut(s) 13, 672, 960
Fsp4HI GCNGC 2 cut(s) 99, 711
FspBI CTAG 1 cut(s) 60
GlaI GCGC 2 cut(s) 37, 426
GluI GCNGC 2 cut(s) 99, 711
GsaI CCCAGC 1 cut(s) 1116
GsuI CTGGAG 2 cut(s) 306, 912
HaeIII GGCC 2 cut(s) 704, 852
HapII CCGG 3 cut(s) 104, 141, 843
HhaI GCGC 2 cut(s) 38, 427
Hin1II CATG 3 cut(s) 514, 538, 560
Hin6I GCGC 2 cut(s) 36, 425
HinP1I GCGC 2 cut(s) 36, 425
HinfI GANTC 9 cut(s) 45, 137, 167, 182, 367, 407, 592, 1167, 1226
HpaII CCGG 3 cut(s) 104, 141, 843
HphI GGTGA 5 cut(s) 155, 704, 809, 908, 1027
Hpy166II GTNNAC 2 cut(s) 322, 371
Hpy188I TCNGA 8 cut(s) 20, 122, 136, 190, 412, 768, 814, 1172
Hpy188III TCNNGA 4 cut(s) 364, 467, 596, 629
Hpy8I GTNNAC 2 cut(s) 322, 371
HpyAV CCTTC 2 cut(s) 190, 539
HpyCH4III ACNGT 1 cut(s) 943
HpyCH4V TGCA 2 cut(s) 26, 1192
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 710, 1118, 1181, 1241
HpyF3I CTNAG 4 cut(s) 165, 777, 860, 983
Hsp92II CATG 3 cut(s) 514, 538, 560
HspAI GCGC 2 cut(s) 36, 425
Kzo9I GATC 7 cut(s) 63, 199, 529, 763, 835, 1128, 1209
LmnI GCTCC 4 cut(s) 8, 106, 112, 892
Lsp1109I GCAGC 1 cut(s) 722
LweI GCATC 4 cut(s) 35, 265, 889, 916
MaeI CTAG 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 937
MalI GATC 7 cut(s) 65, 201, 531, 765, 837, 1130, 1211
MbiI CCGCTC 1 cut(s) 101
MboI GATC 7 cut(s) 63, 199, 529, 763, 835, 1128, 1209
MflI RGATCY 2 cut(s) 63, 199
MhlI GDGCHC 3 cut(s) 13, 672, 960
MluCI AATT 7 cut(s) 116, 347, 500, 696, 731, 750, 791
MlyI GAGTC 3 cut(s) 176, 376, 1161
MmeI TCCRAC 1 cut(s) 577
Mph1103I ATGCAT 1 cut(s) 28
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 3 cut(s) 234, 663, 927
MspI CCGG 3 cut(s) 104, 141, 843
MspR9I CCNGG 2 cut(s) 285, 891
Mva1269I GAATGC 1 cut(s) 427
MvaI CCWGG 2 cut(s) 285, 891
MwoI GCNNNNNNNGC 5 cut(s) 104, 710, 1118, 1181, 1241
NdeI CATATG 1 cut(s) 1153
NdeII GATC 7 cut(s) 63, 199, 529, 763, 835, 1128, 1209
NlaIII CATG 3 cut(s) 514, 538, 560
NlaIV GGNNCC 3 cut(s) 108, 617, 783
NsiI ATGCAT 1 cut(s) 28
NspV TTCGAA 1 cut(s) 417
PaeR7I CTCGAG 1 cut(s) 596
PaqCI CACCTGC 1 cut(s) 1046
PctI GAATGC 1 cut(s) 427
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 6 cut(s) 45, 137, 182, 407, 592, 1226
PfoI TCCNGGA 1 cut(s) 889
PkrI GCNGC 2 cut(s) 100, 712
PleI GAGTC 3 cut(s) 175, 375, 1161
PpsI GAGTC 3 cut(s) 175, 375, 1161
PpuMI RGGWCCY 1 cut(s) 782
Psp124BI GAGCTC 2 cut(s) 13, 672
Psp5II RGGWCCY 1 cut(s) 782
Psp6I CCWGG 2 cut(s) 283, 889
PspFI CCCAGC 1 cut(s) 1112
PspGI CCWGG 2 cut(s) 283, 889
PspN4I GGNNCC 3 cut(s) 108, 617, 783
PspPI GGNCC 3 cut(s) 616, 782, 851
PspPPI RGGWCCY 1 cut(s) 782
PstNI CAGNNNCTG 1 cut(s) 338
PsuI RGATCY 2 cut(s) 63, 199
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
SacI GAGCTC 2 cut(s) 13, 672
SaqAI TTAA 3 cut(s) 234, 663, 927
SatI GCNGC 2 cut(s) 99, 711
Sau3AI GATC 7 cut(s) 63, 199, 529, 763, 835, 1128, 1209
Sau96I GGNCC 3 cut(s) 616, 782, 851
SchI GAGTC 3 cut(s) 176, 376, 1161
ScrFI CCNGG 2 cut(s) 285, 891
SduI GDGCHC 3 cut(s) 13, 672, 960
SfaNI GCATC 4 cut(s) 35, 265, 889, 916
SfcI CTRYAG 1 cut(s) 305
Sfr274I CTCGAG 1 cut(s) 596
SfuI TTCGAA 1 cut(s) 417
SinI GGWCC 2 cut(s) 616, 782
SlaI CTCGAG 1 cut(s) 596
SmlI CTYRAG 3 cut(s) 446, 596, 1081
SmoI CTYRAG 3 cut(s) 446, 596, 1081
Sse9I AATT 7 cut(s) 116, 347, 500, 696, 731, 750, 791
SsiI CCGC 1 cut(s) 99
SspMI CTAG 1 cut(s) 60
SstI GAGCTC 2 cut(s) 13, 672
StyD4I CCNGG 2 cut(s) 283, 889
TaaI ACNGT 1 cut(s) 943
TaqI TCGA 4 cut(s) 152, 363, 417, 597
TasI AATT 7 cut(s) 116, 347, 500, 696, 731, 750, 791
TatI WGTACW 1 cut(s) 456
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 6 cut(s) 45, 137, 182, 407, 592, 1226
Tru1I TTAA 3 cut(s) 234, 663, 927
Tru9I TTAA 3 cut(s) 234, 663, 927
TscAI CASTG 2 cut(s) 169, 948
TseI GCWGC 1 cut(s) 710
TspDTI ATGAA 5 cut(s) 174, 285, 360, 411, 1140
TspRI CASTG 2 cut(s) 169, 948
VpaK11BI GGWCC 2 cut(s) 616, 782
XagI CCTNNNNNAGG 1 cut(s) 1042
XapI RAATTY 3 cut(s) 116, 500, 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 861
XhoI CTCGAG 1 cut(s) 596
XmiI GTMKAC 2 cut(s) 321, 370
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 1 cut(s) 60
Zsp2I ATGCAT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.