RLG00000018997

Mitochondrial ribosomal subunit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006027
Physical Location & Seq
Reverse (-)
44406612 .. 44409271
2660 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018997

Sequence Viewer

Length: 1254 bp
ATGAGGCGGACTCTGCTGAGGAATGTCTCTCTCTTCGCTCGCAACCGCCTCGTCTCCCCAAAACCTAGCCCTGCTTCTTCACTCGCCGCTTCCGCCAGACTCTTCTCCTCCGACAAAAACGACTCGTCGTCTCCCAATGACTCGCCCCCAGAAAGCTCCAATCCCTCTCCTGAGTCGCGCCTCACCCAAGTCTTGAAGAAGGACTCCTCCATTCAACTCGAGGACATCAGTAACAAAGAACTGAAAACAAGAATGGAGGAATACTTGAAGGAAGGTAAGGAGGAGGCGCTCCCATCGATCCTCGAAGCGATTCTGCAGAGGAAATTGGCTGGGAAGCATGAGGACACGGATGATGAACTGGTGGATGATCTGCAAATGCAGCCATTGGAGAATGTTAAGGACCAAGAGTTTGAATCTGATTTCGAAGAAATCCACGACACTGATGAAGAGATTGATGACTTGTATGATGCCAAGGATGTAGTGGTGAAGAAGATGGTGAAGGATGAGTTCTTCAATATGGATGACAAAAAGTGGGATGGTATTGTTCAGGATGCGATCAAGCACGGGATTATGAAGGATATGAGAGAGTGTGAGGAAGTTCTTGAGGATATGCTTAGTTGGGACAAGCTCTTGCCAGATGACATGAAGAAGAAGGTAGAAGAGAAGTTCAATGACCTAGGGGATAGGTGTGAAAGAGGGGAGCTTGAACCTGAAGATGCTTACAAGTTGTTTAAGGAGTTTGAGGACGAGATTGTCATGGAATATACGAAGAAGGTGGAAGCTCAGGATTCTTCACAGTTTGATGAGATAACTGTACCAGACATGAAAAGAGACACAGATGACCCACCAGGTGAAGGGCCAATCCTTAGGTGGCAAACACGAGTGGTCTTTGCTCCTGGTGGTGATGCTTGGCACCCAAAGAATAGAAAAGTGAAGCTGTCTGTTACGGTGAAAGAACTTGGGCTTTCAAAGCATCAATTCCGTAGGCTCAGAGAATTGGTTGGAAAGCGATACAATCCAGGGAAAGATGAGCTCACTATTACTAGTGAGAGGTTTGAACACCGAGAGGAAAACAGAAAGGATTGCCTTAGGACTCTTCTTTCCCTCATTGAGGAAGCTGAGAAAGCTAACAAGCTGGCCGAGGATGCTCGAGTTTTGTATGTCAAGGAGAGATTAAGAGCAAATCCTGCATTCATGGAGAGACTGCGTAACAAGACAATGAGATTGCAAGGACACACCACAGTGACGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S28

Protein Analysis

418

Amino Acids

48.36

Weight (kDa)

5.02

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S28 PF10213 303 - 382 1e-17 Mitochondrial ribosomal subunit protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 752
AccB1I GGYRCC 1 cut(s) 912
AccII CGCG 1 cut(s) 178
AciI CCGC 4 cut(s) 7, 46, 87, 93
AclWI GGATC 1 cut(s) 292
AcoI YGGCCR 1 cut(s) 1137
AcuI CTGAAG 1 cut(s) 732
AdeI CACNNNGTG 1 cut(s) 851
AfaI GTAC 1 cut(s) 816
AfiI CCNNNNNNNGG 2 cut(s) 854, 1111
AgsI TTSAA 9 cut(s) 196, 215, 268, 413, 514, 670, 707, 969, 1058
AhdI GACNNNNNGTC 1 cut(s) 127
AhlI ACTAGT 1 cut(s) 1043
AjnI CCWGG 3 cut(s) 847, 895, 1018
AleI CACNNNNGTG 1 cut(s) 1241
AluBI AGCT 9 cut(s) 156, 628, 703, 782, 937, 1033, 1118, 1127, 1135
AluI AGCT 9 cut(s) 156, 628, 703, 782, 937, 1033, 1118, 1127, 1135
Alw21I GWGCWC 1 cut(s) 1035
Alw26I GTCTC 5 cut(s) 31, 58, 135, 825, 1195
AlwI GGATC 1 cut(s) 292
Ama87I CYCGRG 2 cut(s) 218, 1149
AoxI GGCC 2 cut(s) 857, 1137
ApeKI GCWGC 1 cut(s) 379
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
Asp700I GAANNNNTTC 1 cut(s) 309
AspA2I CCTAGG 1 cut(s) 676
AspLEI GCGC 2 cut(s) 180, 289
AspS9I GGNCC 2 cut(s) 400, 857
AsuHPI GGTGA 6 cut(s) 175, 496, 508, 863, 914, 961
AsuII TTCGAA 1 cut(s) 423
AvaI CYCGRG 2 cut(s) 218, 1149
AvaII GGWCC 1 cut(s) 400
AvrII CCTAGG 1 cut(s) 676
AxyI CCTNAGG 2 cut(s) 866, 1088
BanI GGYRCC 1 cut(s) 912
BanII GRGCYC 1 cut(s) 1035
BauI CACGAG 1 cut(s) 879
Bbv12I GWGCWC 1 cut(s) 1035
BbvCI CCTCAGC 1 cut(s) 17
BbvI GCAGC 1 cut(s) 391
BccI CCATC 3 cut(s) 301, 487, 530
BcgI CGANNNNNNTGC 2 cut(s) 31, 65
BciT130I CCWGG 3 cut(s) 849, 897, 1020
BcoDI GTCTC 5 cut(s) 31, 58, 135, 825, 1195
BcuI ACTAGT 1 cut(s) 1043
BfaI CTAG 3 cut(s) 66, 677, 1044
BfmI CTRYAG 1 cut(s) 314
BfoI RGCGCY 1 cut(s) 290
BisI GCNGC 2 cut(s) 87, 380
BlnI CCTAGG 1 cut(s) 676
BlsI GCNGC 2 cut(s) 88, 381
Bme1390I CCNGG 3 cut(s) 849, 897, 1020
Bme18I GGWCC 1 cut(s) 400
BmeRI GACNNNNNGTC 1 cut(s) 127
BmeT110I CYCGRG 2 cut(s) 218, 1149
BmgT120I GGNCC 2 cut(s) 400, 857
BmiI GGNNCC 1 cut(s) 914
BmrFI CCNGG 3 cut(s) 849, 897, 1020
BmsI GCATC 6 cut(s) 457, 541, 706, 895, 982, 1135
BplI GAGNNNNNCTC 1 cut(s) 27
Bpu10I CCTNAGC 2 cut(s) 17, 783
Bpu14I TTCGAA 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 623
Bsa29I ATCGAT 1 cut(s) 296
BsaJI CCNNGG 4 cut(s) 471, 676, 1019, 1140
Bsc4I CCNNNNNNNGG 2 cut(s) 854, 1111
Bse1I ACTGG 1 cut(s) 363
Bse21I CCTNAGG 2 cut(s) 866, 1088
BseBI CCWGG 3 cut(s) 849, 897, 1020
BseCI ATCGAT 1 cut(s) 296
BseDI CCNNGG 4 cut(s) 471, 676, 1019, 1140
BseGI GGATG 8 cut(s) 355, 370, 481, 508, 526, 541, 556, 1150
BseLI CCNNNNNNNGG 2 cut(s) 854, 1111
BseMII CTCAG 5 cut(s) 8, 162, 797, 1003, 1110
BseNI ACTGG 1 cut(s) 363
BseRI GAGGAG 3 cut(s) 97, 196, 296
BseXI GCAGC 1 cut(s) 391
BseYI CCCAGC 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 178
BshFI GGCC 2 cut(s) 859, 1139
BshNI GGYRCC 1 cut(s) 912
BshVI ATCGAT 1 cut(s) 296
BsiHKAI GWGCWC 1 cut(s) 1035
BsiHKCI CYCGRG 2 cut(s) 218, 1149
BslFI GGGAC 1 cut(s) 635
BslI CCNNNNNNNGG 2 cut(s) 854, 1111
BsmAI GTCTC 5 cut(s) 31, 58, 135, 825, 1195
BsmBI CGTCTC 2 cut(s) 58, 135
BsmFI GGGAC 1 cut(s) 635
BsmI GAATGC 1 cut(s) 1190
BsnI GGCC 2 cut(s) 859, 1139
BsoBI CYCGRG 2 cut(s) 218, 1149
Bsp119I TTCGAA 1 cut(s) 423
Bsp1286I GDGCHC 1 cut(s) 1035
Bsp143I GATC 3 cut(s) 297, 367, 555
BspACI CCGC 4 cut(s) 7, 46, 87, 93
BspANI GGCC 2 cut(s) 859, 1139
BspCNI CTCAG 5 cut(s) 9, 163, 796, 1002, 1111
BspDI ATCGAT 1 cut(s) 296
BspFNI CGCG 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 914
BspMAI CTGCAG 1 cut(s) 318
BspPI GGATC 1 cut(s) 292
BspT104I TTCGAA 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 912
BsrI ACTGG 1 cut(s) 363
BssECI CCNNGG 4 cut(s) 471, 676, 1019, 1140
BssMI GATC 3 cut(s) 297, 367, 555
BssSI CACGAG 1 cut(s) 879
BssT1I CCWWGG 2 cut(s) 471, 676
Bst2BI CACGAG 1 cut(s) 879
Bst2UI CCWGG 3 cut(s) 849, 897, 1020
Bst4CI ACNGT 4 cut(s) 798, 814, 949, 1243
Bst6I CTCTTC 5 cut(s) 38, 107, 441, 654, 1101
BstAPI GCANNNNNTGC 1 cut(s) 1187
BstBI TTCGAA 1 cut(s) 423
BstC8I GCNNGC 2 cut(s) 40, 1137
BstDEI CTNAG 8 cut(s) 17, 171, 614, 783, 866, 989, 1088, 1119
BstENI CCTNNNNNAGG 1 cut(s) 1109
BstF5I GGATG 8 cut(s) 355, 370, 481, 508, 526, 541, 556, 1150
BstFNI CGCG 1 cut(s) 178
BstH2I RGCGCY 1 cut(s) 290
BstHHI GCGC 2 cut(s) 180, 289
BstKTI GATC 3 cut(s) 300, 370, 558
BstMAI GTCTC 5 cut(s) 31, 58, 135, 825, 1195
BstMBI GATC 3 cut(s) 297, 367, 555
BstMWI GCNNNNNNNGC 7 cut(s) 13, 92, 379, 970, 1124, 1145, 1187
BstNI CCWGG 3 cut(s) 849, 897, 1020
BstSCI CCNGG 3 cut(s) 847, 895, 1018
BstSFI CTRYAG 1 cut(s) 314
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 1 cut(s) 391
Bsu15I ATCGAT 1 cut(s) 296
Bsu36I CCTNAGG 2 cut(s) 866, 1088
BsuRI GGCC 2 cut(s) 859, 1139
BsuTUI ATCGAT 1 cut(s) 296
BtsCI GGATG 8 cut(s) 355, 370, 481, 508, 526, 541, 556, 1150
BtsIMutI CAGTG 2 cut(s) 438, 1248
Cac8I GCNNGC 2 cut(s) 40, 1137
CfoI GCGC 2 cut(s) 180, 289
Cfr13I GGNCC 2 cut(s) 400, 857
ClaI ATCGAT 1 cut(s) 296
CsiI ACCWGGT 1 cut(s) 847
Csp6I GTAC 1 cut(s) 815
CviAII CATG 5 cut(s) 338, 643, 757, 823, 1195
CviQI GTAC 1 cut(s) 815
DdeI CTNAG 8 cut(s) 17, 171, 614, 783, 866, 989, 1088, 1119
DpnI GATC 3 cut(s) 299, 369, 557
DpnII GATC 3 cut(s) 297, 367, 555
DraIII CACNNNGTG 1 cut(s) 851
DrdI GACNNNNNNGTC 1 cut(s) 752
DriI GACNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 752
EaeI YGGCCR 1 cut(s) 1137
Eam1104I CTCTTC 5 cut(s) 38, 107, 441, 654, 1101
Eam1105I GACNNNNNGTC 1 cut(s) 127
EarI CTCTTC 5 cut(s) 38, 107, 441, 654, 1101
EciI GGCGGA 2 cut(s) 22, 82
Ecl136II GAGCTC 1 cut(s) 1033
Eco130I CCWWGG 2 cut(s) 471, 676
Eco24I GRGCYC 1 cut(s) 1035
Eco47I GGWCC 1 cut(s) 400
Eco53kI GAGCTC 1 cut(s) 1033
Eco57I CTGAAG 1 cut(s) 732
Eco81I CCTNAGG 2 cut(s) 866, 1088
Eco88I CYCGRG 2 cut(s) 218, 1149
EcoICRI GAGCTC 1 cut(s) 1033
EcoNI CCTNNNNNAGG 1 cut(s) 1109
EcoRII CCWGG 3 cut(s) 847, 895, 1018
EcoT14I CCWWGG 2 cut(s) 471, 676
EcoT38I GRGCYC 1 cut(s) 1035
ErhI CCWWGG 2 cut(s) 471, 676
Esp3I CGTCTC 2 cut(s) 58, 135
FaeI CATG 5 cut(s) 341, 646, 760, 826, 1198
FaqI GGGAC 1 cut(s) 635
FatI CATG 5 cut(s) 337, 642, 756, 822, 1194
Fnu4HI GCNGC 2 cut(s) 87, 380
FokI GGATG 8 cut(s) 362, 377, 488, 515, 533, 548, 563, 1157
FriOI GRGCYC 1 cut(s) 1035
Fsp4HI GCNGC 2 cut(s) 87, 380
FspBI CTAG 3 cut(s) 66, 677, 1044
GlaI GCGC 2 cut(s) 179, 288
GluI GCNGC 2 cut(s) 87, 380
GsaI CCCAGC 1 cut(s) 333
HaeII RGCGCY 1 cut(s) 290
HaeIII GGCC 2 cut(s) 859, 1139
HhaI GCGC 2 cut(s) 180, 289
Hin1II CATG 5 cut(s) 341, 646, 760, 826, 1198
Hin6I GCGC 2 cut(s) 178, 287
HinP1I GCGC 2 cut(s) 178, 287
HphI GGTGA 6 cut(s) 175, 496, 508, 863, 914, 961
Hpy188I TCNGA 3 cut(s) 112, 418, 992
Hpy188III TCNNGA 5 cut(s) 170, 193, 548, 602, 785
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 8 cut(s) 193, 262, 266, 493, 568, 646, 766, 848
HpyCH4III ACNGT 4 cut(s) 798, 814, 949, 1243
HpyCH4V TGCA 5 cut(s) 316, 373, 379, 1190, 1228
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 92, 379, 970, 1124, 1145, 1187
HpyF3I CTNAG 8 cut(s) 17, 171, 614, 783, 866, 989, 1088, 1119
Hsp92II CATG 5 cut(s) 341, 646, 760, 826, 1198
HspAI GCGC 2 cut(s) 178, 287
Kzo9I GATC 3 cut(s) 297, 367, 555
LmnI GCTCC 4 cut(s) 161, 294, 700, 898
Lsp1109I GCAGC 1 cut(s) 391
LweI GCATC 6 cut(s) 457, 541, 706, 895, 982, 1135
MabI ACCWGGT 1 cut(s) 847
MaeI CTAG 3 cut(s) 66, 677, 1044
MaeIII GTNAC 4 cut(s) 230, 943, 1208, 1243
MalI GATC 3 cut(s) 299, 369, 557
MboI GATC 3 cut(s) 297, 367, 555
MhlI GDGCHC 1 cut(s) 1035
MluCI AATT 3 cut(s) 323, 977, 995
MlyI GAGTC 7 cut(s) 4, 93, 116, 134, 182, 197, 1087
MmeI TCCRAC 2 cut(s) 135, 982
MroXI GAANNNNTTC 1 cut(s) 309
MseI TTAA 3 cut(s) 396, 732, 1175
MslI CAYNNNNRTG 1 cut(s) 1241
MspR9I CCNGG 3 cut(s) 849, 897, 1020
Mva1269I GAATGC 1 cut(s) 1190
MvaI CCWGG 3 cut(s) 849, 897, 1020
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 7 cut(s) 13, 92, 379, 970, 1124, 1145, 1187
NdeII GATC 3 cut(s) 297, 367, 555
NlaIII CATG 5 cut(s) 341, 646, 760, 826, 1198
NlaIV GGNNCC 1 cut(s) 914
NmeAIII GCCGAG 1 cut(s) 1165
NmuCI GTSAC 1 cut(s) 1243
NspV TTCGAA 1 cut(s) 423
OliI CACNNNNGTG 1 cut(s) 1241
PaeR7I CTCGAG 2 cut(s) 218, 1149
PctI GAATGC 1 cut(s) 1190
PdmI GAANNNNTTC 1 cut(s) 309
PfeI GAWTC 3 cut(s) 310, 413, 788
PkrI GCNGC 2 cut(s) 88, 381
PleI GAGTC 7 cut(s) 4, 93, 116, 134, 181, 197, 1087
PpsI GAGTC 7 cut(s) 4, 93, 116, 134, 181, 197, 1087
Psp124BI GAGCTC 1 cut(s) 1035
Psp6I CCWGG 3 cut(s) 847, 895, 1018
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 3 cut(s) 847, 895, 1018
PspN4I GGNNCC 1 cut(s) 914
PspPI GGNCC 2 cut(s) 400, 857
PspXI VCTCGAGB 2 cut(s) 218, 1149
PstI CTGCAG 1 cut(s) 318
RsaI GTAC 1 cut(s) 816
RsaNI GTAC 1 cut(s) 815
RseI CAYNNNNRTG 1 cut(s) 1241
SacI GAGCTC 1 cut(s) 1035
SaqAI TTAA 3 cut(s) 396, 732, 1175
SatI GCNGC 2 cut(s) 87, 380
Sau3AI GATC 3 cut(s) 297, 367, 555
Sau96I GGNCC 2 cut(s) 400, 857
SchI GAGTC 7 cut(s) 4, 93, 116, 134, 182, 197, 1087
ScrFI CCNGG 3 cut(s) 849, 897, 1020
SduI GDGCHC 1 cut(s) 1035
SexAI ACCWGGT 1 cut(s) 847
SfaNI GCATC 6 cut(s) 457, 541, 706, 895, 982, 1135
SfcI CTRYAG 1 cut(s) 314
Sfr274I CTCGAG 2 cut(s) 218, 1149
SfuI TTCGAA 1 cut(s) 423
SinI GGWCC 1 cut(s) 400
SlaI CTCGAG 2 cut(s) 218, 1149
SmiMI CAYNNNNRTG 1 cut(s) 1241
SmlI CTYRAG 3 cut(s) 218, 602, 1149
SmoI CTYRAG 3 cut(s) 218, 602, 1149
SpeI ACTAGT 1 cut(s) 1043
Sse9I AATT 3 cut(s) 323, 977, 995
SsiI CCGC 4 cut(s) 7, 46, 87, 93
SspMI CTAG 3 cut(s) 66, 677, 1044
SstI GAGCTC 1 cut(s) 1035
StyD4I CCNGG 3 cut(s) 847, 895, 1018
StyI CCWWGG 2 cut(s) 471, 676
TaaI ACNGT 4 cut(s) 798, 814, 949, 1243
TaqI TCGA 5 cut(s) 219, 296, 303, 423, 1150
TasI AATT 3 cut(s) 323, 977, 995
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 3 cut(s) 310, 413, 788
Tru1I TTAA 3 cut(s) 396, 732, 1175
Tru9I TTAA 3 cut(s) 396, 732, 1175
TscAI CASTG 2 cut(s) 445, 1248
TseFI GTSAC 1 cut(s) 1243
TseI GCWGC 1 cut(s) 379
Tsp45I GTSAC 1 cut(s) 1243
TspDTI ATGAA 6 cut(s) 369, 459, 587, 659, 839, 1183
TspGWI ACGGA 2 cut(s) 362, 971
TspRI CASTG 2 cut(s) 445, 1248
VpaK11BI GGWCC 1 cut(s) 400
XagI CCTNNNNNAGG 1 cut(s) 1109
XcmI CCANNNNNNNNNTGG 2 cut(s) 478, 867
XhoI CTCGAG 2 cut(s) 218, 1149
XmaJI CCTAGG 1 cut(s) 676
XmnI GAANNNNTTC 1 cut(s) 309
XspI CTAG 3 cut(s) 66, 677, 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.