MD06G1220500.v1.1

Mitochondrial ribosomal subunit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006027
Physical Location & Seq
Reverse (-)
35234109 .. 35236754
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1220500.v1.1.491

Sequence Viewer

Length: 1260 bp
ATGAGACGAACTCTGCTGAGCAATCTCTCTCTCTACACTCGCAATCGCCTTCTCTCTCCTCAAACATTCAACCCAAATCCTACCCCCTCACACGTCCCACTCGCCCGTTCGACTCAGTCCCTGCTCAGCCGGTTCTTCTCCTCGGAGGGCGACTCCTCCGCCGAAAGCCCCAATCCGGCTGCTGATTCGAGCTTGATCCCAACCAAAAAGAAAGATGCTTCCGTCGAAGTTAAGGACGTCAATAACAAAGAGCTGAAGGAGCGGATAGATAAATATTTTAAGGGTGACGAGGAGGCGCTTCCGGCCATACTCGAAGCGATTCTGCAGAGGAGATTGGCTGGGAAGCATGAGGAAACGGATGATGAGCTGATGGATGAATTGCAAATGCAGCCGTTGGATGATGTTAAGGACCAAGAGTTTGAATCGGATTTTGAGGAATTGCATGACACGGATGAAGAGATTGATGACTTGTATAACGCAAAGGATATAGTCATGAAGAGGATGTCAAACGATGAGTACTTCAACATGGATCCCAAGAAGTGGGATGATATTGTTGAGGATGCAATTAAGCACGGGATCATGAAAAATACGAAGGAGTGTGAGGAGATTCTCGAGGATATGCTTAGCTGGGATAAGCTCCTACCAGATGACATGAAAAGGAAGGTAGAAGAGAAGTTCAATGACTTAGGGGATAAGTGTGAAAGAGGGGAGCTTCAACCTGAAGAAGCTTATGAGTTGTTCAAGGAGTTTGAGGATGAGATTGTCATGGACTATTTGCAGAAGATGGAAGCCAACGGCCCCCCACAATTTGATGAGGCTGATGTGTATGACAAGAAGGATCTAGCTGACCCACCAGGCGAAGGGCCAATCCTTAGGTGGCAGACACGGGTAGTCTTTGCTCCTGGTGGTGATTCCTGGCATCCCAAGAATAGAAAAGTGAAACTGTCTGTTACAGTGAAAGAACTCGGGCTTTCAAAGCATCAATTCCGTCGGCTCAGAGACTTGGTTGGAAAGCGGTACCATCCAGGGAAAGATGAGCTTACAATTACTAGTGAGAGATTTGAACACCGAGAAGAAAATAGAAAGGACTGCCTCAGGACACTTCTTTCCCTCATTGAGGAAGCTGGGAAAGCAAACAAACTGGCTGAGGATGCTCGACTTTCGTATGTCAAGAAGAGATTAAGAGCAAATCCTGCATTCATGGAGAGGCTGCAGAACAAGACCATGAAATTGCAAGGAAAGAGCGCAGTACCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S28

Protein Analysis

420

Amino Acids

48.54

Weight (kDa)

5.06

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S28 PF10213 305 - 388 4e-17 Mitochondrial ribosomal subunit protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 240
Acc65I GGTACC 1 cut(s) 1017
AccB1I GGYRCC 1 cut(s) 1017
AccB7I CCANNNNNTGG 1 cut(s) 540
AccBSI CCGCTC 1 cut(s) 262
AciI CCGC 3 cut(s) 159, 262, 1015
AclWI GGATC 5 cut(s) 190, 524, 537, 584, 846
AcoI YGGCCR 1 cut(s) 303
AcuI CTGAAG 2 cut(s) 275, 741
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 3 cut(s) 518, 1019, 1251
AfiI CCNNNNNNNGG 4 cut(s) 175, 540, 860, 1117
AflIII ACRYGT 1 cut(s) 91
AgsI TTSAA 8 cut(s) 70, 422, 523, 679, 716, 742, 975, 1064
AhlI ACTAGT 1 cut(s) 1049
AjiI CACGTC 1 cut(s) 94
AjnI CCWGG 4 cut(s) 853, 901, 914, 1024
Alw26I GTCTC 1 cut(s) 993
AlwI GGATC 5 cut(s) 190, 524, 537, 584, 846
AlwNI CAGNNNCTG 2 cut(s) 121, 1253
Ama87I CYCGRG 2 cut(s) 611, 965
AoxI GGCC 3 cut(s) 303, 796, 863
ApeKI GCWGC 3 cut(s) 179, 388, 1210
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
Asp700I GAANNNNTTC 1 cut(s) 318
Asp718I GGTACC 1 cut(s) 1017
AspLEI GCGC 2 cut(s) 298, 1247
AspS9I GGNCC 3 cut(s) 409, 797, 863
AsuHPI GGTGA 2 cut(s) 296, 920
AvaI CYCGRG 2 cut(s) 611, 965
AvaII GGWCC 1 cut(s) 409
AxyI CCTNAGG 2 cut(s) 872, 1094
BamHI GGATCC 1 cut(s) 529
BanI GGYRCC 1 cut(s) 1017
BbvCI CCTCAGC 1 cut(s) 1146
BbvI GCAGC 3 cut(s) 166, 400, 1197
BccI CCATC 3 cut(s) 364, 778, 1029
BceAI ACGGC 2 cut(s) 376, 811
BciT130I CCWGG 4 cut(s) 855, 903, 916, 1026
BcoDI GTCTC 1 cut(s) 993
BcuI ACTAGT 1 cut(s) 1049
BfaI CTAG 2 cut(s) 842, 1050
BfmI CTRYAG 2 cut(s) 323, 1211
BfoI RGCGCY 1 cut(s) 299
BisI GCNGC 3 cut(s) 180, 389, 1211
BlpI GCTNAGC 3 cut(s) 17, 125, 623
BlsI GCNGC 3 cut(s) 181, 390, 1212
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 4 cut(s) 855, 903, 916, 1026
Bme18I GGWCC 1 cut(s) 409
BmeT110I CYCGRG 2 cut(s) 611, 965
BmgBI CACGTC 1 cut(s) 94
BmgT120I GGNCC 3 cut(s) 409, 797, 863
BmiI GGNNCC 3 cut(s) 531, 799, 1019
BmrFI CCNGG 4 cut(s) 855, 903, 916, 1026
BmsI GCATC 5 cut(s) 205, 550, 928, 988, 1141
BplI GAGNNNNNCTC 3 cut(s) 27, 137, 169
Bpu10I CCTNAGC 1 cut(s) 1146
Bpu1102I GCTNAGC 3 cut(s) 17, 125, 623
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 2 cut(s) 141, 1025
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 540, 860, 1117
Bse118I RCCGGY 1 cut(s) 129
Bse1I ACTGG 1 cut(s) 1146
Bse21I CCTNAGG 2 cut(s) 872, 1094
BseBI CCWGG 4 cut(s) 855, 903, 916, 1026
BseDI CCNNGG 2 cut(s) 141, 1025
BseLI CCNNNNNNNGG 4 cut(s) 175, 540, 860, 1117
BseMII CTCAG 6 cut(s) 8, 128, 139, 1009, 1108, 1137
BseNI ACTGG 1 cut(s) 1146
BseRI GAGGAG 6 cut(s) 48, 130, 145, 305, 343, 617
BseXI GCAGC 3 cut(s) 166, 400, 1197
BseYI CCCAGC 3 cut(s) 338, 627, 1124
BshFI GGCC 3 cut(s) 305, 798, 865
BshNI GGYRCC 1 cut(s) 1017
BsiHKCI CYCGRG 2 cut(s) 611, 965
BsiSI CCGG 3 cut(s) 130, 176, 302
BslFI GGGAC 2 cut(s) 80, 103
BslI CCNNNNNNNGG 4 cut(s) 175, 540, 860, 1117
BsmAI GTCTC 1 cut(s) 993
BsmFI GGGAC 2 cut(s) 80, 103
BsmI GAATGC 1 cut(s) 1196
BsnI GGCC 3 cut(s) 305, 798, 865
BsoBI CYCGRG 2 cut(s) 611, 965
Bsp143I GATC 4 cut(s) 195, 529, 576, 838
Bsp1720I GCTNAGC 3 cut(s) 17, 125, 623
BspACI CCGC 3 cut(s) 159, 262, 1015
BspANI GGCC 3 cut(s) 305, 798, 865
BspCNI CTCAG 6 cut(s) 9, 127, 138, 1008, 1107, 1138
BspHI TCATGA 2 cut(s) 492, 579
BspLI GGNNCC 3 cut(s) 531, 799, 1019
BspMAI CTGCAG 2 cut(s) 327, 1215
BspPI GGATC 5 cut(s) 190, 524, 537, 584, 846
BspT107I GGYRCC 1 cut(s) 1017
BsrBI CCGCTC 1 cut(s) 262
BsrFI RCCGGY 1 cut(s) 129
BsrI ACTGG 1 cut(s) 1146
BssAI RCCGGY 1 cut(s) 129
BssECI CCNNGG 2 cut(s) 141, 1025
BssMI GATC 4 cut(s) 195, 529, 576, 838
BssNI GRCGYC 1 cut(s) 237
Bst2UI CCWGG 4 cut(s) 855, 903, 916, 1026
Bst4CI ACNGT 2 cut(s) 945, 955
Bst6I CTCTTC 4 cut(s) 450, 491, 663, 1169
BstACI GRCGYC 1 cut(s) 237
BstAPI GCANNNNNTGC 2 cut(s) 1193, 1253
BstDEI CTNAG 9 cut(s) 17, 114, 125, 623, 685, 872, 995, 1094, 1146
BstENI CCTNNNNNAGG 1 cut(s) 1115
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 2 cut(s) 298, 1247
BstKTI GATC 4 cut(s) 198, 532, 579, 841
BstMAI GTCTC 1 cut(s) 993
BstMBI GATC 4 cut(s) 195, 529, 576, 838
BstMWI GCNNNNNNNGC 8 cut(s) 259, 302, 388, 976, 1130, 1151, 1193, 1253
BstNI CCWGG 4 cut(s) 855, 903, 916, 1026
BstSCI CCNGG 4 cut(s) 853, 901, 914, 1024
BstSFI CTRYAG 2 cut(s) 323, 1211
BstV1I GCAGC 3 cut(s) 166, 400, 1197
BstX2I RGATCY 2 cut(s) 529, 838
BstYI RGATCY 2 cut(s) 529, 838
Bsu36I CCTNAGG 2 cut(s) 872, 1094
BsuRI GGCC 3 cut(s) 305, 798, 865
BtrI CACGTC 1 cut(s) 94
BtsIMutI CAGTG 1 cut(s) 960
CaiI CAGNNNCTG 2 cut(s) 121, 1253
CciI TCATGA 2 cut(s) 492, 579
CfoI GCGC 2 cut(s) 298, 1247
Cfr10I RCCGGY 1 cut(s) 129
Cfr13I GGNCC 3 cut(s) 409, 797, 863
Csp6I GTAC 3 cut(s) 517, 1018, 1250
CviAII CATG 9 cut(s) 347, 443, 493, 526, 580, 652, 766, 1201, 1225
CviQI GTAC 3 cut(s) 517, 1018, 1250
DdeI CTNAG 9 cut(s) 17, 114, 125, 623, 685, 872, 995, 1094, 1146
DpnI GATC 4 cut(s) 197, 531, 578, 840
DpnII GATC 4 cut(s) 195, 529, 576, 838
EaeI YGGCCR 1 cut(s) 303
Eam1104I CTCTTC 4 cut(s) 450, 491, 663, 1169
EarI CTCTTC 4 cut(s) 450, 491, 663, 1169
EciI GGCGGA 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 409
Eco57I CTGAAG 2 cut(s) 275, 741
Eco81I CCTNAGG 2 cut(s) 872, 1094
Eco88I CYCGRG 2 cut(s) 611, 965
EcoNI CCTNNNNNAGG 1 cut(s) 1115
EcoRII CCWGG 4 cut(s) 853, 901, 914, 1024
FaeI CATG 9 cut(s) 350, 446, 496, 529, 583, 655, 769, 1204, 1228
FalI AAGNNNNNCTT 2 cut(s) 1023, 1055
FaqI GGGAC 2 cut(s) 80, 103
FatI CATG 9 cut(s) 346, 442, 492, 525, 579, 651, 765, 1200, 1224
Fnu4HI GCNGC 3 cut(s) 180, 389, 1211
Fsp4HI GCNGC 3 cut(s) 180, 389, 1211
FspBI CTAG 2 cut(s) 842, 1050
GlaI GCGC 2 cut(s) 297, 1246
GluI GCNGC 3 cut(s) 180, 389, 1211
GsaI CCCAGC 3 cut(s) 342, 631, 1128
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 3 cut(s) 305, 798, 865
HapII CCGG 3 cut(s) 130, 176, 302
HhaI GCGC 2 cut(s) 298, 1247
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 9 cut(s) 350, 446, 496, 529, 583, 655, 769, 1204, 1228
Hin6I GCGC 2 cut(s) 296, 1245
HinP1I GCGC 2 cut(s) 296, 1245
HindIII AAGCTT 1 cut(s) 726
HinfI GANTC 7 cut(s) 112, 152, 185, 319, 422, 607, 911
HpaII CCGG 3 cut(s) 130, 176, 302
HphI GGTGA 2 cut(s) 296, 920
Hpy188I TCNGA 3 cut(s) 145, 427, 998
Hpy188III TCNNGA 5 cut(s) 493, 580, 611, 1096, 1171
Hpy99I CGWCG 2 cut(s) 227, 993
HpyAV CCTTC 6 cut(s) 59, 250, 586, 655, 829, 854
HpyCH4III ACNGT 2 cut(s) 945, 955
HpyCH4IV ACGT 2 cut(s) 93, 237
HpyCH4V TGCA 9 cut(s) 325, 382, 388, 442, 563, 778, 1196, 1213, 1234
HpyF10VI GCNNNNNNNGC 8 cut(s) 259, 302, 388, 976, 1130, 1151, 1193, 1253
HpyF3I CTNAG 9 cut(s) 17, 114, 125, 623, 685, 872, 995, 1094, 1146
HpySE526I ACGT 2 cut(s) 93, 237
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 9 cut(s) 350, 446, 496, 529, 583, 655, 769, 1204, 1228
HspAI GCGC 2 cut(s) 296, 1245
KpnI GGTACC 1 cut(s) 1021
Kzo9I GATC 4 cut(s) 195, 529, 576, 838
LmnI GCTCC 4 cut(s) 259, 642, 709, 904
Lsp1109I GCAGC 3 cut(s) 166, 400, 1197
LweI GCATC 5 cut(s) 205, 550, 928, 988, 1141
MaeI CTAG 2 cut(s) 842, 1050
MaeII ACGT 2 cut(s) 93, 237
MaeIII GTNAC 2 cut(s) 284, 949
MalI GATC 4 cut(s) 197, 531, 578, 840
MbiI CCGCTC 1 cut(s) 262
MboI GATC 4 cut(s) 195, 529, 576, 838
MboII GAAGA 8 cut(s) 127, 467, 508, 680, 734, 793, 1085, 1186
MflI RGATCY 2 cut(s) 529, 838
MluCI AATT 7 cut(s) 377, 437, 564, 806, 983, 1044, 1229
MlyI GAGTC 2 cut(s) 106, 146
MmeI TCCRAC 2 cut(s) 375, 988
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 5 cut(s) 231, 279, 405, 567, 1181
MspI CCGG 3 cut(s) 130, 176, 302
MspR9I CCNGG 4 cut(s) 855, 903, 916, 1026
Mva1269I GAATGC 1 cut(s) 1196
MvaI CCWGG 4 cut(s) 855, 903, 916, 1026
MwoI GCNNNNNNNGC 8 cut(s) 259, 302, 388, 976, 1130, 1151, 1193, 1253
NdeII GATC 4 cut(s) 195, 529, 576, 838
NlaIII CATG 9 cut(s) 350, 446, 496, 529, 583, 655, 769, 1204, 1228
NlaIV GGNNCC 3 cut(s) 531, 799, 1019
NmuCI GTSAC 1 cut(s) 284
PaeR7I CTCGAG 1 cut(s) 611
PagI TCATGA 2 cut(s) 492, 579
PctI GAATGC 1 cut(s) 1196
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 5 cut(s) 185, 319, 422, 607, 911
PflFI GACNNNGTC 1 cut(s) 115
PflMI CCANNNNNTGG 1 cut(s) 540
PkrI GCNGC 3 cut(s) 181, 390, 1212
PleI GAGTC 2 cut(s) 106, 146
PpsI GAGTC 2 cut(s) 106, 146
Psp6I CCWGG 4 cut(s) 853, 901, 914, 1024
PspFI CCCAGC 3 cut(s) 338, 627, 1124
PspGI CCWGG 4 cut(s) 853, 901, 914, 1024
PspN4I GGNNCC 3 cut(s) 531, 799, 1019
PspPI GGNCC 3 cut(s) 409, 797, 863
PstI CTGCAG 2 cut(s) 327, 1215
PstNI CAGNNNCTG 2 cut(s) 121, 1253
PsuI RGATCY 2 cut(s) 529, 838
PsyI GACNNNGTC 1 cut(s) 115
RsaI GTAC 3 cut(s) 518, 1019, 1251
RsaNI GTAC 3 cut(s) 517, 1018, 1250
SaqAI TTAA 5 cut(s) 231, 279, 405, 567, 1181
SatI GCNGC 3 cut(s) 180, 389, 1211
Sau3AI GATC 4 cut(s) 195, 529, 576, 838
Sau96I GGNCC 3 cut(s) 409, 797, 863
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 2 cut(s) 106, 146
ScrFI CCNGG 4 cut(s) 855, 903, 916, 1026
SfaNI GCATC 5 cut(s) 205, 550, 928, 988, 1141
SfcI CTRYAG 2 cut(s) 323, 1211
Sfr274I CTCGAG 1 cut(s) 611
SinI GGWCC 1 cut(s) 409
SlaI CTCGAG 1 cut(s) 611
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
SpeI ACTAGT 1 cut(s) 1049
Sse9I AATT 7 cut(s) 377, 437, 564, 806, 983, 1044, 1229
SsiI CCGC 3 cut(s) 159, 262, 1015
SspI AATATT 1 cut(s) 275
SspMI CTAG 2 cut(s) 842, 1050
StyD4I CCNGG 4 cut(s) 853, 901, 914, 1024
TaaI ACNGT 2 cut(s) 945, 955
TaiI ACGT 2 cut(s) 96, 240
TaqI TCGA 6 cut(s) 110, 188, 225, 312, 612, 1156
TasI AATT 7 cut(s) 377, 437, 564, 806, 983, 1044, 1229
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 5 cut(s) 185, 319, 422, 607, 911
Tru1I TTAA 5 cut(s) 231, 279, 405, 567, 1181
Tru9I TTAA 5 cut(s) 231, 279, 405, 567, 1181
TscAI CASTG 1 cut(s) 960
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 3 cut(s) 179, 388, 1210
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 7 cut(s) 390, 468, 509, 596, 668, 1189, 1241
TspGWI ACGGA 4 cut(s) 211, 371, 464, 977
TspRI CASTG 1 cut(s) 960
Tth111I GACNNNGTC 1 cut(s) 115
Van91I CCANNNNNTGG 1 cut(s) 540
VpaK11BI GGWCC 1 cut(s) 409
XagI CCTNNNNNAGG 1 cut(s) 1115
XcmI CCANNNNNNNNNTGG 1 cut(s) 873
XhoI CTCGAG 1 cut(s) 611
XmnI GAANNNNTTC 1 cut(s) 318
XspI CTAG 2 cut(s) 842, 1050
ZraI GACGTC 1 cut(s) 238
ZrmI AGTACT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.