AT3G19630

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007961
Physical Location & Seq
Reverse (-)
6818387 .. 6820762
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G19630.1

Sequence Viewer

Length: 1119 bp
ATGAAGTTGAAATCGGTGTTCGATGCTTCGGAAATCAAATCGGAATTTGAATCAGCGGGAATAAACCCTAAATTCGCGATTCAAATCTGGAAGTATGTAATTCAGAATCCTGATTGCGTTTGGGACGAGATTCCTTCATTGCCTTCCGCTGCATACTCTCTTCTCCATTCCAAGTTCAAGACTCTTACTTCGTCTCTTCACTCGCTATTTCATTCCTCCGATGGCACCACCTCAAAGCTTCTCATCAAGCTCCAGAATGGAGCTTTTGTGGAAGCTGTGGTAATGAGATACGATACTCGGTTGGGGATGTTAGGAGGGAAACCACGTCCTGGAGGTATAAGATCTACGTTATGTATTTCATCTCAGGTTGGTTGTAAGATGGGTTGCACATTCTGTGCTACTGGTACCATGGGATTTAAAAGCAATTTAACATCTGGAGAAATTGTGGAGCAGCTTGTTCACGCCTCTCGCATTGCTGATATACGCAACATCGTTTTCATGGGAATGGGAGAACCTTTAAATAACTACAATGCTGTTGTTGAAGCTGTTCGTGTCATGTTAAACCAACCATTTCAGCTGTCGCCCAAAAGAATTACCATATCAACTGTCGGAATTGTTCACGCAATTAACAAGCTACACAATGATCTACCCGGTGTAAGTTTAGCAGTATCCCTCCATGCACCAGTTCAAGAAATCCGCTGCCAGATCATGCCAGCAGCTAGAGCCTTTCCTTTACAAAAGCTTATGGATGCACTTCAAACTTTCCAAAAGAACAGTCAACAAAAGATCTTCATTGAGTACATAATGCTTGATGGAGTAAATGATCAAGAGCAGCACGCTCATCTACTAGGCGAATTGCTAAAGACATTTCAAGTGGTCATAAATTTGATACCATTTAATCCAATTGGATCCACAAGCCAATTCGAAACAAGCAGCATACAAGGCGTGTCAAGATTCCAGAAAATCCTGAGGGAAACATACAAGATCCGGACTACAATTCGCAAAGAAATGGGTCAGGATATTAGCGGCGCTTGCGGTCAGCTAGTGGTGAACCAACCAGACATCAAAAAGACTCCTGGAACTGTGGAACTTAGAGACATAGAAGATCTGCTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

372

Amino Acids

41.18

Weight (kDa)

8.87

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 120 - 279 3.8e-14 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 404
AccB1I GGYRCC 2 cut(s) 224, 404
AccB7I CCANNNNNTGG 1 cut(s) 329
AccII CGCG 1 cut(s) 77
AccIII TCCGGA 1 cut(s) 987
AciI CCGC 5 cut(s) 56, 147, 697, 1026, 1035
AclWI GGATC 3 cut(s) 903, 916, 979
AcsI RAATTY 3 cut(s) 44, 71, 883
AfaI GTAC 2 cut(s) 406, 800
AfiI CCNNNNNNNGG 1 cut(s) 329
AgsI TTSAA 8 cut(s) 10, 50, 83, 178, 542, 689, 758, 872
AjiI CACGTC 1 cut(s) 326
AjnI CCWGG 2 cut(s) 328, 1075
Alw26I GTCTC 2 cut(s) 198, 1089
AlwI GGATC 3 cut(s) 903, 916, 979
Aor13HI TCCGGA 1 cut(s) 987
ApeKI GCWGC 6 cut(s) 149, 451, 699, 716, 832, 933
ApoI RAATTY 3 cut(s) 44, 71, 883
ArsI GACNNNNNNTTYG 4 cut(s) 172, 204, 760, 792
Asp700I GAANNNNTTC 1 cut(s) 546
Asp718I GGTACC 1 cut(s) 404
AspLEI GCGC 1 cut(s) 1031
AsuC2I CCSGG 1 cut(s) 651
AsuHPI GGTGA 1 cut(s) 1060
AsuII TTCGAA 1 cut(s) 924
AxyI CCTNAGG 1 cut(s) 968
BamHI GGATCC 1 cut(s) 908
BanI GGYRCC 2 cut(s) 224, 404
BbvI GCAGC 6 cut(s) 136, 463, 686, 728, 844, 945
BccI CCATC 3 cut(s) 215, 373, 806
BciT130I CCWGG 2 cut(s) 330, 1077
BciVI GTATCC 1 cut(s) 679
BclI TGATCA 1 cut(s) 823
BcnI CCSGG 1 cut(s) 651
BcoDI GTCTC 2 cut(s) 198, 1089
BfaI CTAG 3 cut(s) 720, 848, 1043
BfoI RGCGCY 1 cut(s) 1032
BfuI GTATCC 1 cut(s) 679
BglII AGATCT 3 cut(s) 341, 786, 1105
BisI GCNGC 7 cut(s) 150, 452, 700, 717, 833, 934, 1027
BlsI GCNGC 7 cut(s) 151, 453, 701, 718, 834, 935, 1028
Bme1390I CCNGG 3 cut(s) 330, 651, 1077
BmgBI CACGTC 1 cut(s) 326
BmiI GGNNCC 3 cut(s) 226, 406, 910
BmrFI CCNGG 3 cut(s) 330, 651, 1077
BmsI GCATC 2 cut(s) 13, 739
BpmI CTGGAG 3 cut(s) 236, 351, 456
Bpu14I TTCGAA 1 cut(s) 924
BpuMI CCSGG 1 cut(s) 651
BsaBI GATNNNNATC 1 cut(s) 83
BsaJI CCNNGG 1 cut(s) 408
BsaWI WCCGGW 1 cut(s) 987
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 429, 459
Bsc4I CCNNNNNNNGG 1 cut(s) 329
Bse1I ACTGG 2 cut(s) 406, 683
Bse21I CCTNAGG 1 cut(s) 968
Bse3DI GCAATG 2 cut(s) 137, 471
Bse8I GATNNNNATC 1 cut(s) 83
BseAI TCCGGA 1 cut(s) 987
BseBI CCWGG 2 cut(s) 330, 1077
BseDI CCNNGG 1 cut(s) 408
BseGI GGATG 2 cut(s) 312, 754
BseJI GATNNNNATC 1 cut(s) 83
BseLI CCNNNNNNNGG 1 cut(s) 329
BseMI GCAATG 2 cut(s) 137, 471
BseMII CTCAG 2 cut(s) 377, 959
BseNI ACTGG 2 cut(s) 406, 683
BseXI GCAGC 6 cut(s) 136, 463, 686, 728, 844, 945
Bsh1236I CGCG 1 cut(s) 77
BshNI GGYRCC 2 cut(s) 224, 404
BsiSI CCGG 2 cut(s) 651, 988
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 1 cut(s) 329
BsmAI GTCTC 2 cut(s) 198, 1089
BsmBI CGTCTC 1 cut(s) 198
BsmFI GGGAC 1 cut(s) 137
Bsp119I TTCGAA 1 cut(s) 924
Bsp13I TCCGGA 1 cut(s) 987
Bsp143I GATC 8 cut(s) 341, 643, 705, 786, 823, 908, 984, 1105
Bsp19I CCATGG 1 cut(s) 408
Bsp68I TCGCGA 1 cut(s) 77
BspACI CCGC 5 cut(s) 56, 147, 697, 1026, 1035
BspCNI CTCAG 2 cut(s) 376, 960
BspEI TCCGGA 1 cut(s) 987
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 3 cut(s) 226, 406, 910
BspPI GGATC 3 cut(s) 903, 916, 979
BspT104I TTCGAA 1 cut(s) 924
BspT107I GGYRCC 2 cut(s) 224, 404
BsrDI GCAATG 2 cut(s) 137, 471
BsrI ACTGG 2 cut(s) 406, 683
BssECI CCNNGG 1 cut(s) 408
BssMI GATC 8 cut(s) 341, 643, 705, 786, 823, 908, 984, 1105
BssT1I CCWWGG 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 330, 1077
Bst4CI ACNGT 3 cut(s) 607, 776, 1084
Bst6I CTCTTC 2 cut(s) 165, 201
BstBI TTCGAA 1 cut(s) 924
BstC8I GCNNGC 3 cut(s) 714, 837, 1033
BstDEI CTNAG 3 cut(s) 363, 968, 1091
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 2 cut(s) 312, 754
BstFNI CGCG 1 cut(s) 77
BstH2I RGCGCY 1 cut(s) 1032
BstHHI GCGC 1 cut(s) 1031
BstKTI GATC 8 cut(s) 344, 646, 708, 789, 826, 911, 987, 1108
BstMAI GTCTC 2 cut(s) 198, 1089
BstMBI GATC 8 cut(s) 341, 643, 705, 786, 823, 908, 984, 1105
BstMWI GCNNNNNNNGC 3 cut(s) 722, 942, 1032
BstNI CCWGG 2 cut(s) 330, 1077
BstSCI CCNGG 3 cut(s) 328, 649, 1075
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 6 cut(s) 136, 463, 686, 728, 844, 945
BstX2I RGATCY 5 cut(s) 341, 786, 908, 984, 1105
BstYI RGATCY 5 cut(s) 341, 786, 908, 984, 1105
Bsu36I CCTNAGG 1 cut(s) 968
BsuI GTATCC 1 cut(s) 679
BtgI CCRYGG 1 cut(s) 408
BtrI CACGTC 1 cut(s) 326
BtsCI GGATG 2 cut(s) 312, 754
BtuMI TCGCGA 1 cut(s) 77
Cac8I GCNNGC 3 cut(s) 714, 837, 1033
CfoI GCGC 1 cut(s) 1031
Csp6I GTAC 2 cut(s) 405, 799
CviAII CATG 5 cut(s) 409, 499, 556, 677, 709
CviQI GTAC 2 cut(s) 405, 799
DdeI CTNAG 3 cut(s) 363, 968, 1091
DpnI GATC 8 cut(s) 343, 645, 707, 788, 825, 910, 986, 1107
DpnII GATC 8 cut(s) 341, 643, 705, 786, 823, 908, 984, 1105
DraI TTTAAA 2 cut(s) 418, 519
Eam1104I CTCTTC 2 cut(s) 165, 201
EarI CTCTTC 2 cut(s) 165, 201
Eco130I CCWWGG 1 cut(s) 408
Eco81I CCTNAGG 1 cut(s) 968
EcoRII CCWGG 2 cut(s) 328, 1075
EcoT14I CCWWGG 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 408
Esp3I CGTCTC 1 cut(s) 198
FaeI CATG 5 cut(s) 412, 502, 559, 680, 712
FalI AAGNNNNNCTT 1 cut(s) 1095
FaqI GGGAC 1 cut(s) 137
FatI CATG 5 cut(s) 408, 498, 555, 676, 708
FauI CCCGC 1 cut(s) 49
FbaI TGATCA 1 cut(s) 823
Fnu4HI GCNGC 7 cut(s) 150, 452, 700, 717, 833, 934, 1027
FokI GGATG 2 cut(s) 319, 761
Fsp4HI GCNGC 7 cut(s) 150, 452, 700, 717, 833, 934, 1027
FspBI CTAG 3 cut(s) 720, 848, 1043
GlaI GCGC 1 cut(s) 1030
GluI GCNGC 7 cut(s) 150, 452, 700, 717, 833, 934, 1027
GsuI CTGGAG 3 cut(s) 236, 351, 456
HaeII RGCGCY 1 cut(s) 1032
HapII CCGG 2 cut(s) 651, 988
HhaI GCGC 1 cut(s) 1031
Hin1II CATG 5 cut(s) 412, 502, 559, 680, 712
Hin6I GCGC 1 cut(s) 1029
HinP1I GCGC 1 cut(s) 1029
HincII GTYRAC 1 cut(s) 779
HindII GTYRAC 1 cut(s) 779
HindIII AAGCTT 2 cut(s) 236, 740
HinfI GANTC 7 cut(s) 50, 79, 106, 130, 181, 954, 1072
HpaII CCGG 2 cut(s) 651, 988
HphI GGTGA 1 cut(s) 1060
Hpy166II GTNNAC 4 cut(s) 460, 619, 779, 1051
Hpy188I TCNGA 5 cut(s) 31, 43, 105, 220, 611
Hpy8I GTNNAC 4 cut(s) 460, 619, 779, 1051
HpyAV CCTTC 2 cut(s) 144, 153
HpyCH4III ACNGT 3 cut(s) 607, 776, 1084
HpyCH4IV ACGT 2 cut(s) 325, 347
HpyCH4V TGCA 4 cut(s) 152, 387, 680, 752
HpyF10VI GCNNNNNNNGC 3 cut(s) 722, 942, 1032
HpyF3I CTNAG 3 cut(s) 363, 968, 1091
HpySE526I ACGT 2 cut(s) 325, 347
Hsp92II CATG 5 cut(s) 412, 502, 559, 680, 712
HspAI GCGC 1 cut(s) 1029
Kpn2I TCCGGA 1 cut(s) 987
KpnI GGTACC 1 cut(s) 408
Ksp22I TGATCA 1 cut(s) 823
Kzo9I GATC 8 cut(s) 341, 643, 705, 786, 823, 908, 984, 1105
LmnI GCTCC 3 cut(s) 255, 260, 448
Lsp1109I GCAGC 6 cut(s) 136, 463, 686, 728, 844, 945
LweI GCATC 2 cut(s) 13, 739
MaeI CTAG 3 cut(s) 720, 848, 1043
MaeII ACGT 2 cut(s) 325, 347
MalI GATC 8 cut(s) 343, 645, 707, 788, 825, 910, 986, 1107
MboI GATC 8 cut(s) 341, 643, 705, 786, 823, 908, 984, 1105
MboII GAAGA 4 cut(s) 152, 188, 781, 1115
MfeI CAATTG 1 cut(s) 903
MflI RGATCY 5 cut(s) 341, 786, 908, 984, 1105
MlyI GAGTC 2 cut(s) 175, 1066
MmeI TCCRAC 1 cut(s) 589
MnlI CCTC 7 cut(s) 226, 241, 308, 326, 475, 683, 963
MroI TCCGGA 1 cut(s) 987
MroXI GAANNNNTTC 1 cut(s) 546
MseI TTAA 6 cut(s) 417, 428, 518, 560, 627, 897
MslI CAYNNNNRTG 1 cut(s) 503
MspA1I CMGCKG 4 cut(s) 56, 149, 577, 699
MspI CCGG 2 cut(s) 651, 988
MspR9I CCNGG 3 cut(s) 330, 651, 1077
MunI CAATTG 1 cut(s) 903
MvaI CCWGG 2 cut(s) 330, 1077
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 3 cut(s) 722, 942, 1032
NciI CCSGG 1 cut(s) 651
NcoI CCATGG 1 cut(s) 408
NdeII GATC 8 cut(s) 341, 643, 705, 786, 823, 908, 984, 1105
NlaIII CATG 5 cut(s) 412, 502, 559, 680, 712
NlaIV GGNNCC 3 cut(s) 226, 406, 910
NruI TCGCGA 1 cut(s) 77
NspV TTCGAA 1 cut(s) 924
PdmI GAANNNNTTC 1 cut(s) 546
PfeI GAWTC 5 cut(s) 50, 79, 106, 130, 954
PflMI CCANNNNNTGG 1 cut(s) 329
PfoI TCCNGGA 2 cut(s) 328, 1075
PkrI GCNGC 7 cut(s) 151, 453, 701, 718, 834, 935, 1028
PleI GAGTC 2 cut(s) 175, 1066
PpsI GAGTC 2 cut(s) 175, 1066
Psp6I CCWGG 2 cut(s) 328, 1075
PspGI CCWGG 2 cut(s) 328, 1075
PspN4I GGNNCC 3 cut(s) 226, 406, 910
PsuI RGATCY 5 cut(s) 341, 786, 908, 984, 1105
PvuII CAGCTG 1 cut(s) 577
RruI TCGCGA 1 cut(s) 77
RsaI GTAC 2 cut(s) 406, 800
RsaNI GTAC 2 cut(s) 405, 799
RseI CAYNNNNRTG 1 cut(s) 503
SaqAI TTAA 6 cut(s) 417, 428, 518, 560, 627, 897
SatI GCNGC 7 cut(s) 150, 452, 700, 717, 833, 934, 1027
Sau3AI GATC 8 cut(s) 341, 643, 705, 786, 823, 908, 984, 1105
SchI GAGTC 2 cut(s) 175, 1066
ScrFI CCNGG 3 cut(s) 330, 651, 1077
SfaNI GCATC 2 cut(s) 13, 739
SfuI TTCGAA 1 cut(s) 924
SmiMI CAYNNNNRTG 1 cut(s) 503
SsiI CCGC 5 cut(s) 56, 147, 697, 1026, 1035
SspMI CTAG 3 cut(s) 720, 848, 1043
StyD4I CCNGG 3 cut(s) 328, 649, 1075
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 3 cut(s) 607, 776, 1084
TaiI ACGT 2 cut(s) 328, 350
TaqI TCGA 2 cut(s) 21, 924
TatI WGTACW 1 cut(s) 798
TauI GCSGC 1 cut(s) 1029
TfiI GAWTC 5 cut(s) 50, 79, 106, 130, 954
Tru1I TTAA 6 cut(s) 417, 428, 518, 560, 627, 897
Tru9I TTAA 6 cut(s) 417, 428, 518, 560, 627, 897
TseI GCWGC 6 cut(s) 149, 451, 699, 716, 832, 933
TspDTI ATGAA 6 cut(s) 17, 126, 200, 348, 487, 781
Van91I CCANNNNNTGG 1 cut(s) 329
XapI RAATTY 3 cut(s) 44, 71, 883
XmnI GAANNNNTTC 1 cut(s) 546
XspI CTAG 3 cut(s) 720, 848, 1043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.