RLG00000014990
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007961
Physical Location & Seq
Reverse (-)
60369676 .. 60373120
3445 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014990

Sequence Viewer

Length: 1494 bp
ATGGAAGGGTTGCTTCCCCTTCTATACCATGCCATCAAGAAGCGAAGATCAAAACACAAGCATAGATGCTTTTTGGAGAGCAATTCGAAAAGTAGCTACCGTCTCCTCGGGTGTGGCCATGACCTAACCTTGGAAGGCTTGTCTTATTGTCAAAAAGGATCCGAGTTCCAACCTCTGGTCACTGCTGAATTATTAGAGCAGCGATCCGCACTTGACTTTAATCATTCGTGTAGCTACGAGGAAGAAGATGGTGTTTCTGCGGGGGTTCCTTTTGTGTCAAGTTCCTCAAAAGATAGCGAAGTCATAACTCATAAGCCACCACTCCGCCTCCGTCACCGCAACCCCCCAATCATGCAAAGTGCTCGCAAAACGACGCCGTTCCGGTCAATTTTCGACGCTGGAGCTCTGAAATCAGAGCTGGAAAACTCCGGGATCAAGCCCAGCTTCATGCCCTACATATGGAAGCAGGTGATCCAAAACCCCTCCGCCCAACTCGACCAAATCCCCTCCTTGCCCTCCGCCGCATACCCTCTTCTAAACTCCAAATTCAAAACCCTAACCTCCTCCGTCCACTCCGCCACCGACTCCTCCGACCACCTCACCACCAAGCTCCTCATCAAGCTCCAGAACGGCGCTCTGGTGGAGGCCGTGATCATGAGGTACGATAGCAGCTTGGGGAAGCACAATGGCAAGTCCCACCACGGCGGCGCAAGGTCCACATTGTGCGTATCGTCTCAGGTTGGTTGTAAAATGGGTTGCACGTTCTGTGCCACTGGGAGCATGGGGTTTAAGAGCAATTTGACCTCTGGGGAGATTGTGGAGCAGTTGGTTCATGCTTCTCGCATTTCGCCTATACGCAATGTTGTTTTCATGGGAATGGGGGAACCGTTGAATAACTATAATGCCTTAGTGGAAGCGATTCGTGTCATGACTGGATTACCATTTCAGCTGTCGCCAAAGAGAATCACAGTGTCAACGGTTGGTATTATTCATGCTATCAACAAGTTCCATAATGATCTCCCTGGACTGAATTTAGCAGTTTCACTGCATGCACCTGTCCAAGAAATTCGTTGTCAGATAATGCCTGCAGCTCGGGCCTTTCCCTTAGTAAAACTTATGGATTCACTGCAACAATATCAAAAGAACAGTCAGCAGAAAATTTTCATTGAATACATAATGCTTGACGGAGTGAACGATGAAGAAGAACATGCGCACCAACTTGGGAAATTGCTAGATACATTTCAAGTGGTAATTAATTTAATACCTTTTAACCCAATTGGTAGTCTGAGCAAATTCAAAACCACTGCCGAAGAAAAAGTATCTGTTTTTCAGAAGATTCTAAGAGGTACGTATGGCATCCGAACAACCGTTCGCAAGGAAATGGGTCAGGACATAAGTGGAGCATGTGGTCAGTTGGTGGTGAACCTACCCAATAAGAGGTCAAGTGACAACGCAGTTCCTCTAACTGATATAGAAGATCTTCAGGTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

498

Amino Acids

54.92

Weight (kDa)

9.07

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 244 - 404 3.5e-11 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 457
Acc16I TGCGCA 1 cut(s) 1212
Acc36I ACCTGC 1 cut(s) 457
AccB7I CCANNNNNTGG 1 cut(s) 175
AciI CCGC 9 cut(s) 207, 260, 325, 337, 486, 519, 522, 576, 705
AclWI GGATC 5 cut(s) 153, 166, 198, 440, 466
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 5 cut(s) 545, 1030, 1065, 1158, 1292
AcuI CTGAAG 1 cut(s) 1466
AcyI GRCGYC 1 cut(s) 374
AdeI CACNNNGTG 1 cut(s) 723
AfaI GTAC 2 cut(s) 662, 1348
AfiI CCNNNNNNNGG 4 cut(s) 130, 175, 459, 1437
AgsI TTSAA 5 cut(s) 550, 892, 1169, 1244, 1297
AjnI CCWGG 1 cut(s) 1021
Alw21I GWGCWC 2 cut(s) 364, 406
Alw26I GTCTC 2 cut(s) 107, 738
AlwI GGATC 5 cut(s) 153, 166, 198, 440, 466
Ama87I CYCGRG 2 cut(s) 107, 1092
AoxI GGCC 3 cut(s) 115, 645, 1095
ApeKI GCWGC 3 cut(s) 199, 669, 1088
ApoI RAATTY 5 cut(s) 545, 1030, 1065, 1158, 1292
ArsI GACNNNNNNTTYG 2 cut(s) 1132, 1164
AseI ATTAAT 1 cut(s) 1254
Asp700I GAANNNNTTC 3 cut(s) 918, 1160, 1479
AspLEI GCGC 3 cut(s) 635, 710, 1213
AspS9I GGNCC 2 cut(s) 714, 1095
AsuC2I CCSGG 1 cut(s) 430
AsuHPI GGTGA 4 cut(s) 326, 481, 592, 1432
AsuII TTCGAA 1 cut(s) 86
AvaI CYCGRG 2 cut(s) 107, 1092
AvaII GGWCC 1 cut(s) 714
BalI TGGCCA 1 cut(s) 117
BamHI GGATCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 406
Bbv12I GWGCWC 2 cut(s) 364, 406
BbvI GCAGC 3 cut(s) 211, 681, 1100
BccI CCATC 2 cut(s) 41, 242
BceAI ACGGC 4 cut(s) 361, 632, 646, 718
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciT130I CCWGG 1 cut(s) 1023
BclI TGATCA 1 cut(s) 651
BcnI CCSGG 1 cut(s) 430
BcoDI GTCTC 2 cut(s) 107, 738
BfaI CTAG 1 cut(s) 1232
BfmI CTRYAG 1 cut(s) 1086
BfoI RGCGCY 1 cut(s) 636
BfuAI ACCTGC 1 cut(s) 457
BglII AGATCT 1 cut(s) 1477
BisI GCNGC 5 cut(s) 200, 522, 670, 706, 1089
BlsI GCNGC 5 cut(s) 201, 523, 671, 707, 1090
Bme1390I CCNGG 2 cut(s) 430, 1023
Bme18I GGWCC 1 cut(s) 714
BmeT110I CYCGRG 2 cut(s) 107, 1092
BmgT120I GGNCC 2 cut(s) 714, 1095
BmiI GGNNCC 3 cut(s) 160, 267, 885
BmrFI CCNGG 2 cut(s) 430, 1023
BmrI ACTGGG 1 cut(s) 783
BmsI GCATC 2 cut(s) 56, 1365
BmuI ACTGGG 1 cut(s) 783
BpmI CTGGAG 2 cut(s) 420, 608
Bpu14I TTCGAA 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 430
BsaAI YACGTR 1 cut(s) 1350
BsaHI GRCGYC 1 cut(s) 374
BsaJI CCNNGG 4 cut(s) 106, 129, 700, 1021
BsaWI WCCGGW 1 cut(s) 381
BsaXI ACNNNNNCTCC 2 cut(s) 312, 342
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 175, 459, 1437
Bse1I ACTGG 2 cut(s) 778, 937
Bse3DI GCAATG 1 cut(s) 865
BseBI CCWGG 1 cut(s) 1023
BseDI CCNNGG 4 cut(s) 106, 129, 700, 1021
BseGI GGATG 1 cut(s) 1356
BseLI CCNNNNNNNGG 4 cut(s) 130, 175, 459, 1437
BseMI GCAATG 1 cut(s) 865
BseMII CTCAG 2 cut(s) 749, 1277
BseNI ACTGG 2 cut(s) 778, 937
BseRI GAGGAG 4 cut(s) 95, 553, 577, 602
BseXI GCAGC 3 cut(s) 211, 681, 1100
BseYI CCCAGC 1 cut(s) 440
BshFI GGCC 3 cut(s) 117, 647, 1097
BsiHKAI GWGCWC 2 cut(s) 364, 406
BsiHKCI CYCGRG 2 cut(s) 107, 1092
BsiSI CCGG 2 cut(s) 382, 429
BslFI GGGAC 1 cut(s) 679
BslI CCNNNNNNNGG 4 cut(s) 130, 175, 459, 1437
BsmAI GTCTC 2 cut(s) 107, 738
BsmBI CGTCTC 2 cut(s) 107, 738
BsmFI GGGAC 1 cut(s) 679
BsnI GGCC 3 cut(s) 117, 647, 1097
BsoBI CYCGRG 2 cut(s) 107, 1092
Bsp119I TTCGAA 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 364, 406
Bsp143I GATC 8 cut(s) 47, 158, 203, 432, 471, 651, 1015, 1477
BspACI CCGC 9 cut(s) 207, 260, 325, 337, 486, 519, 522, 576, 705
BspANI GGCC 3 cut(s) 117, 647, 1097
BspCNI CTCAG 2 cut(s) 748, 1278
BspHI TCATGA 2 cut(s) 654, 927
BspLI GGNNCC 3 cut(s) 160, 267, 885
BspMAI CTGCAG 1 cut(s) 1090
BspMI ACCTGC 1 cut(s) 457
BspPI GGATC 5 cut(s) 153, 166, 198, 440, 466
BspT104I TTCGAA 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 865
BsrI ACTGG 2 cut(s) 778, 937
BssECI CCNNGG 4 cut(s) 106, 129, 700, 1021
BssMI GATC 8 cut(s) 47, 158, 203, 432, 471, 651, 1015, 1477
BssNI GRCGYC 1 cut(s) 374
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 1023
Bst4CI ACNGT 6 cut(s) 101, 888, 970, 979, 1148, 1369
Bst6I CTCTTC 1 cut(s) 537
BstACI GRCGYC 1 cut(s) 374
BstBAI YACGTR 1 cut(s) 1350
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 3 cut(s) 364, 1050, 1086
BstDEI CTNAG 5 cut(s) 735, 907, 1105, 1286, 1340
BstDSI CCRYGG 1 cut(s) 700
BstF5I GGATG 1 cut(s) 1356
BstH2I RGCGCY 1 cut(s) 636
BstHHI GCGC 3 cut(s) 635, 710, 1213
BstKTI GATC 8 cut(s) 50, 161, 206, 435, 474, 654, 1018, 1480
BstMAI GTCTC 2 cut(s) 107, 738
BstMBI GATC 8 cut(s) 47, 158, 203, 432, 471, 651, 1015, 1477
BstMWI GCNNNNNNNGC 1 cut(s) 1094
BstNI CCWGG 1 cut(s) 1023
BstNSI RCATGY 3 cut(s) 1052, 1211, 1407
BstSCI CCNGG 2 cut(s) 428, 1021
BstSFI CTRYAG 1 cut(s) 1086
BstSNI TACGTA 1 cut(s) 1350
BstV1I GCAGC 3 cut(s) 211, 681, 1100
BstX2I RGATCY 2 cut(s) 158, 1477
BstYI RGATCY 2 cut(s) 158, 1477
BsuRI GGCC 3 cut(s) 117, 647, 1097
BtgI CCRYGG 1 cut(s) 700
BtsCI GGATG 1 cut(s) 1356
BtsI GCAGTG 4 cut(s) 180, 1043, 1124, 1302
BtsIMutI CAGTG 6 cut(s) 180, 771, 975, 1043, 1124, 1302
BveI ACCTGC 1 cut(s) 457
Cac8I GCNNGC 3 cut(s) 364, 1050, 1086
CciI TCATGA 2 cut(s) 654, 927
CfoI GCGC 3 cut(s) 635, 710, 1213
Cfr13I GGNCC 2 cut(s) 714, 1095
CseI GACGC 2 cut(s) 382, 404
Csp6I GTAC 2 cut(s) 661, 1347
CviQI GTAC 2 cut(s) 661, 1347
DdeI CTNAG 5 cut(s) 735, 907, 1105, 1286, 1340
DpnI GATC 8 cut(s) 49, 160, 205, 434, 473, 653, 1017, 1479
DpnII GATC 8 cut(s) 47, 158, 203, 432, 471, 651, 1015, 1477
DraIII CACNNNGTG 1 cut(s) 723
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
EciI GGCGGA 4 cut(s) 314, 475, 508, 565
Ecl136II GAGCTC 1 cut(s) 404
Eco105I TACGTA 1 cut(s) 1350
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 714
Eco53kI GAGCTC 1 cut(s) 404
Eco57I CTGAAG 1 cut(s) 1466
Eco88I CYCGRG 2 cut(s) 107, 1092
EcoICRI GAGCTC 1 cut(s) 404
EcoRII CCWGG 1 cut(s) 1021
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 2 cut(s) 107, 738
FalI AAGNNNNNCTT 3 cut(s) 29, 428, 460
FaqI GGGAC 1 cut(s) 679
FauI CCCGC 1 cut(s) 253
FauNDI CATATG 1 cut(s) 458
FbaI TGATCA 1 cut(s) 651
Fnu4HI GCNGC 5 cut(s) 200, 522, 670, 706, 1089
FokI GGATG 1 cut(s) 1343
FriOI GRGCYC 1 cut(s) 406
Fsp4HI GCNGC 5 cut(s) 200, 522, 670, 706, 1089
FspAI RTGCGCAY 1 cut(s) 1212
FspBI CTAG 1 cut(s) 1232
FspI TGCGCA 1 cut(s) 1212
GlaI GCGC 3 cut(s) 634, 709, 1212
GluI GCNGC 5 cut(s) 200, 522, 670, 706, 1089
GsaI CCCAGC 1 cut(s) 444
GsuI CTGGAG 2 cut(s) 420, 608
HaeII RGCGCY 1 cut(s) 636
HaeIII GGCC 3 cut(s) 117, 647, 1097
HapII CCGG 2 cut(s) 382, 429
HgaI GACGC 2 cut(s) 382, 404
HhaI GCGC 3 cut(s) 635, 710, 1213
Hin1I GRCGYC 1 cut(s) 374
Hin6I GCGC 3 cut(s) 633, 708, 1211
HinP1I GCGC 3 cut(s) 633, 708, 1211
HincII GTYRAC 1 cut(s) 975
HindII GTYRAC 1 cut(s) 975
HinfI GANTC 5 cut(s) 584, 919, 963, 1121, 1336
HpaII CCGG 2 cut(s) 382, 429
HphI GGTGA 4 cut(s) 326, 481, 592, 1432
Hpy166II GTNNAC 5 cut(s) 571, 717, 975, 1192, 1423
Hpy188I TCNGA 9 cut(s) 163, 408, 415, 592, 1077, 1287, 1332, 1361, 1490
Hpy188III TCNNGA 5 cut(s) 37, 625, 655, 928, 1388
Hpy8I GTNNAC 5 cut(s) 571, 717, 975, 1192, 1423
Hpy99I CGWCG 2 cut(s) 376, 398
HpyAV CCTTC 2 cut(s) 29, 128
HpyCH4III ACNGT 6 cut(s) 101, 888, 970, 979, 1148, 1369
HpyCH4IV ACGT 2 cut(s) 761, 1349
HpyCH4V TGCA 6 cut(s) 355, 759, 1048, 1052, 1088, 1129
HpyF10VI GCNNNNNNNGC 1 cut(s) 1094
HpyF3I CTNAG 5 cut(s) 735, 907, 1105, 1286, 1340
HpySE526I ACGT 2 cut(s) 761, 1349
Hsp92I GRCGYC 1 cut(s) 374
HspAI GCGC 3 cut(s) 633, 708, 1211
Ksp22I TGATCA 1 cut(s) 651
Kzo9I GATC 8 cut(s) 47, 158, 203, 432, 471, 651, 1015, 1477
LmnI GCTCC 6 cut(s) 401, 615, 627, 777, 820, 1400
Lsp1109I GCAGC 3 cut(s) 211, 681, 1100
LweI GCATC 2 cut(s) 56, 1365
MaeI CTAG 1 cut(s) 1232
MaeII ACGT 2 cut(s) 761, 1349
MaeIII GTNAC 3 cut(s) 178, 332, 1445
MalI GATC 8 cut(s) 49, 160, 205, 434, 473, 653, 1017, 1479
MboI GATC 8 cut(s) 47, 158, 203, 432, 471, 651, 1015, 1477
MfeI CAATTG 1 cut(s) 1275
MflI RGATCY 2 cut(s) 158, 1477
MhlI GDGCHC 2 cut(s) 364, 406
MlsI TGGCCA 1 cut(s) 117
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 578
MmeI TCCRAC 2 cut(s) 193, 615
Mox20I TGGCCA 1 cut(s) 117
MroXI GAANNNNTTC 3 cut(s) 918, 1160, 1479
MscI TGGCCA 1 cut(s) 117
MseI TTAA 5 cut(s) 219, 789, 1254, 1259, 1269
MslI CAYNNNNRTG 1 cut(s) 875
Msp20I TGGCCA 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 949
MspI CCGG 2 cut(s) 382, 429
MspR9I CCNGG 2 cut(s) 430, 1023
MunI CAATTG 1 cut(s) 1275
MvaI CCWGG 1 cut(s) 1023
MwoI GCNNNNNNNGC 1 cut(s) 1094
NciI CCSGG 1 cut(s) 430
NdeI CATATG 1 cut(s) 458
NdeII GATC 8 cut(s) 47, 158, 203, 432, 471, 651, 1015, 1477
NlaIV GGNNCC 3 cut(s) 160, 267, 885
NmuCI GTSAC 3 cut(s) 178, 332, 1445
NsbI TGCGCA 1 cut(s) 1212
NspI RCATGY 3 cut(s) 1052, 1211, 1407
NspV TTCGAA 1 cut(s) 86
PaeI GCATGC 1 cut(s) 1052
PagI TCATGA 2 cut(s) 654, 927
PaqCI CACCTGC 1 cut(s) 457
PcsI WCGNNNNNNNCGW 1 cut(s) 1191
PdmI GAANNNNTTC 3 cut(s) 918, 1160, 1479
PfeI GAWTC 4 cut(s) 919, 963, 1121, 1336
PflMI CCANNNNNTGG 1 cut(s) 175
PfoI TCCNGGA 1 cut(s) 428
PkrI GCNGC 5 cut(s) 201, 523, 671, 707, 1090
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
Ppu21I YACGTR 1 cut(s) 1350
PshBI ATTAAT 1 cut(s) 1254
Psp124BI GAGCTC 1 cut(s) 406
Psp6I CCWGG 1 cut(s) 1021
PspFI CCCAGC 1 cut(s) 440
PspGI CCWGG 1 cut(s) 1021
PspN4I GGNNCC 3 cut(s) 160, 267, 885
PspPI GGNCC 2 cut(s) 714, 1095
PstI CTGCAG 1 cut(s) 1090
PsuI RGATCY 2 cut(s) 158, 1477
PvuII CAGCTG 1 cut(s) 949
RsaI GTAC 2 cut(s) 662, 1348
RsaNI GTAC 2 cut(s) 661, 1347
RseI CAYNNNNRTG 1 cut(s) 875
SacI GAGCTC 1 cut(s) 406
SaqAI TTAA 5 cut(s) 219, 789, 1254, 1259, 1269
SatI GCNGC 5 cut(s) 200, 522, 670, 706, 1089
Sau3AI GATC 8 cut(s) 47, 158, 203, 432, 471, 651, 1015, 1477
Sau96I GGNCC 2 cut(s) 714, 1095
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 2 cut(s) 430, 1023
SduI GDGCHC 2 cut(s) 364, 406
SfaNI GCATC 2 cut(s) 56, 1365
SfcI CTRYAG 1 cut(s) 1086
SfuI TTCGAA 1 cut(s) 86
SinI GGWCC 1 cut(s) 714
SmiMI CAYNNNNRTG 1 cut(s) 875
SnaBI TACGTA 1 cut(s) 1350
SphI GCATGC 1 cut(s) 1052
SsiI CCGC 9 cut(s) 207, 260, 325, 337, 486, 519, 522, 576, 705
SspMI CTAG 1 cut(s) 1232
SstI GAGCTC 1 cut(s) 406
StyD4I CCNGG 2 cut(s) 428, 1021
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 6 cut(s) 101, 888, 970, 979, 1148, 1369
TaiI ACGT 2 cut(s) 764, 1352
TaqI TCGA 3 cut(s) 86, 393, 495
TauI GCSGC 2 cut(s) 524, 708
TfiI GAWTC 4 cut(s) 919, 963, 1121, 1336
Tru1I TTAA 5 cut(s) 219, 789, 1254, 1259, 1269
Tru9I TTAA 5 cut(s) 219, 789, 1254, 1259, 1269
TscAI CASTG 6 cut(s) 187, 778, 975, 1050, 1131, 1309
TseFI GTSAC 3 cut(s) 178, 332, 1445
TseI GCWGC 3 cut(s) 199, 669, 1088
Tsp45I GTSAC 3 cut(s) 178, 332, 1445
TspDTI ATGAA 6 cut(s) 436, 821, 859, 980, 1153, 1212
TspGWI ACGGA 3 cut(s) 320, 556, 1200
TspRI CASTG 6 cut(s) 187, 778, 975, 1050, 1131, 1309
Van91I CCANNNNNTGG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 714
VspI ATTAAT 1 cut(s) 1254
XapI RAATTY 5 cut(s) 545, 1030, 1065, 1158, 1292
XceI RCATGY 3 cut(s) 1052, 1211, 1407
XcmI CCANNNNNNNNNTGG 1 cut(s) 778
XmnI GAANNNNTTC 3 cut(s) 918, 1160, 1479
XspI CTAG 1 cut(s) 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.