Rroxscaffold_7G00211690
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007961
Physical Location & Seq
Reverse (-)
62123066 .. 62125693
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00211690.1

Sequence Viewer

Length: 1143 bp
ATGCAGAGTGCTCGCAAAACGACGCCGTTCCGGTCAATTTTCGACGCCGGAGCTCTGAAATCAGAGCTGGAGAACTCCGGGATCAAGCCCAGCTTCATGCCCTACATATGGAAGCAGGTGATCCAAAACCCCTCCGCCCAACTCGACCAAATCCCCTCCTTGCCCTCCGCCGCATACCCTCTTCTCAACTCCAAATTCAAAACCCTAACCTCCTCCGTCCACTCCGCCACCGACTCCTCCGACCACCTCACCACCAAGCTCCTCATCAAGCTCCAGAGCGGCGCTCTGGTGGAGTCCGTGATCATGAGGTACGATAGCAGCTTGGGGAAGCACAATGGCAAGTCCCACCACGGCGGCGCAAGGTCCACATTGTGCGTTTCGTCTCAGGTTGGTTGTAAAATGGGTTGCACGTTCTGCGCCACTGGGAGCATGGGGTTTAAGAGCAATTTGACCTCTGGGGAGATTGTGGAGCAGTTGGTTCATGCTTCTCGCATTTCGCCTATACGCAATGTAGTATTCATGGGAATGGGGGAACCGTTGAATAACTATAATGCCTTAGTGGAAGCGATTCGTGTCATGACTGGATTACCATTTCAGCTGTCGCCAAAGAGAATCACAGTGTCAACGGTTGGTATTATTCATGCTATCAACAAGTTCCATAATGATCTCCCTGGACTGAATTTAGCAGTTTCACTGCACGCACCTGTCCAAGAAATTCGTTGTCAGATAATGCCTGCAGCTAGGGCCTTTCCCTTAGTAAAACTTATGGATTCACTGCAACAATATCAAAAGAACAGTCAGCAGAAAATTTTCATTGAATACATAATGCTTGACGGAGTGAACGATGAAGAAGAACATGCGCACCAGCTTGGGAAATTGCTAGATACATTTCAAGTGGTAATTAATTTAATACCTTTTAACCCAATTGGTAGTCTGAGCAAATTCAAAACCACTGCCGAAGAAAAAGTATCTGTTTTTCAGAAGATTCTAAGAGGTACGTATGGCATCCGAACAACCGTTCGCAAGGAAATGGGTCAGGACATAAGTGGAGCATGTGGTCAGTTGGTGGTGAACCTACCCAGTAAGAGGTCAAGTGACAACGCAGTTCCTCTAACTGATATAGAAGATCTTCAGGTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

380

Amino Acids

41.68

Weight (kDa)

9.21

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 127 - 287 1.9e-11 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 106
Acc16I TGCGCA 1 cut(s) 861
Acc36I ACCTGC 1 cut(s) 106
AccBSI CCGCTC 1 cut(s) 279
AciI CCGC 6 cut(s) 135, 168, 171, 225, 279, 354
AclWI GGATC 2 cut(s) 89, 115
AcsI RAATTY 5 cut(s) 194, 679, 714, 807, 941
AcuI CTGAAG 1 cut(s) 1115
AcyI GRCGYC 2 cut(s) 23, 45
AdeI CACNNNGTG 1 cut(s) 372
AfaI GTAC 2 cut(s) 311, 997
AfiI CCNNNNNNNGG 2 cut(s) 108, 1086
AgsI TTSAA 5 cut(s) 199, 541, 818, 893, 946
AjnI CCWGG 1 cut(s) 670
AluBI AGCT 9 cut(s) 53, 67, 93, 259, 271, 321, 598, 740, 868
AluI AGCT 9 cut(s) 53, 67, 93, 259, 271, 321, 598, 740, 868
Alw21I GWGCWC 2 cut(s) 13, 55
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 2 cut(s) 89, 115
AoxI GGCC 1 cut(s) 744
ApeKI GCWGC 2 cut(s) 318, 737
ApoI RAATTY 5 cut(s) 194, 679, 714, 807, 941
ArsI GACNNNNNNTTYG 2 cut(s) 781, 813
AseI ATTAAT 1 cut(s) 903
Asp700I GAANNNNTTC 3 cut(s) 567, 809, 1128
AspLEI GCGC 4 cut(s) 284, 359, 419, 862
AspS9I GGNCC 2 cut(s) 363, 744
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 3 cut(s) 130, 241, 1081
AvaII GGWCC 1 cut(s) 363
BanII GRGCYC 1 cut(s) 55
Bbv12I GWGCWC 2 cut(s) 13, 55
BbvI GCAGC 2 cut(s) 330, 749
BceAI ACGGC 2 cut(s) 10, 367
BcgI CGANNNNNNTGC 1 cut(s) 27
BciT130I CCWGG 1 cut(s) 672
BclI TGATCA 1 cut(s) 300
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 387
BfaI CTAG 2 cut(s) 741, 881
BfmI CTRYAG 1 cut(s) 735
BfoI RGCGCY 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 106
BglII AGATCT 1 cut(s) 1126
BisI GCNGC 5 cut(s) 171, 280, 319, 355, 738
BlsI GCNGC 5 cut(s) 172, 281, 320, 356, 739
Bme1390I CCNGG 2 cut(s) 79, 672
Bme18I GGWCC 1 cut(s) 363
BmgT120I GGNCC 2 cut(s) 363, 744
BmiI GGNNCC 1 cut(s) 534
BmrFI CCNGG 2 cut(s) 79, 672
BmrI ACTGGG 2 cut(s) 432, 1074
BmsI GCATC 1 cut(s) 1014
BmuI ACTGGG 2 cut(s) 432, 1074
BplI GAGNNNNNCTC 2 cut(s) 268, 300
BpmI CTGGAG 2 cut(s) 89, 257
BpuMI CCSGG 1 cut(s) 79
BsaAI YACGTR 1 cut(s) 999
BsaHI GRCGYC 2 cut(s) 23, 45
BsaJI CCNNGG 2 cut(s) 349, 670
BsaWI WCCGGW 1 cut(s) 30
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 1086
Bse1I ACTGG 3 cut(s) 427, 586, 1080
Bse3DI GCAATG 1 cut(s) 514
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 2 cut(s) 349, 670
BseGI GGATG 1 cut(s) 1005
BseLI CCNNNNNNNGG 2 cut(s) 108, 1086
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 2 cut(s) 398, 926
BseNI ACTGG 3 cut(s) 427, 586, 1080
BseRI GAGGAG 3 cut(s) 202, 226, 251
BseXI GCAGC 2 cut(s) 330, 749
BseYI CCCAGC 1 cut(s) 89
BsgI GTGCAG 1 cut(s) 680
BshFI GGCC 1 cut(s) 746
BsiHKAI GWGCWC 2 cut(s) 13, 55
BsiSI CCGG 3 cut(s) 31, 48, 78
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 2 cut(s) 108, 1086
BsmAI GTCTC 1 cut(s) 387
BsmBI CGTCTC 1 cut(s) 387
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 1 cut(s) 746
Bsp1286I GDGCHC 2 cut(s) 13, 55
Bsp143I GATC 5 cut(s) 81, 120, 300, 664, 1126
BspACI CCGC 6 cut(s) 135, 168, 171, 225, 279, 354
BspANI GGCC 1 cut(s) 746
BspCNI CTCAG 2 cut(s) 397, 927
BspHI TCATGA 2 cut(s) 303, 576
BspLI GGNNCC 1 cut(s) 534
BspMAI CTGCAG 1 cut(s) 739
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 2 cut(s) 89, 115
BsrBI CCGCTC 1 cut(s) 279
BsrDI GCAATG 1 cut(s) 514
BsrI ACTGG 3 cut(s) 427, 586, 1080
BssECI CCNNGG 2 cut(s) 349, 670
BssMI GATC 5 cut(s) 81, 120, 300, 664, 1126
BssNI GRCGYC 2 cut(s) 23, 45
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 5 cut(s) 537, 619, 628, 797, 1018
Bst6I CTCTTC 1 cut(s) 186
BstACI GRCGYC 2 cut(s) 23, 45
BstAPI GCANNNNNTGC 1 cut(s) 414
BstBAI YACGTR 1 cut(s) 999
BstC8I GCNNGC 3 cut(s) 13, 699, 735
BstDEI CTNAG 5 cut(s) 384, 556, 754, 935, 989
BstDSI CCRYGG 1 cut(s) 349
BstF5I GGATG 1 cut(s) 1005
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 4 cut(s) 284, 359, 419, 862
BstKTI GATC 5 cut(s) 84, 123, 303, 667, 1129
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 5 cut(s) 81, 120, 300, 664, 1126
BstMWI GCNNNNNNNGC 2 cut(s) 414, 743
BstNI CCWGG 1 cut(s) 672
BstNSI RCATGY 2 cut(s) 860, 1056
BstSCI CCNGG 2 cut(s) 77, 670
BstSFI CTRYAG 1 cut(s) 735
BstSNI TACGTA 1 cut(s) 999
BstV1I GCAGC 2 cut(s) 330, 749
BstX2I RGATCY 1 cut(s) 1126
BstYI RGATCY 1 cut(s) 1126
BsuRI GGCC 1 cut(s) 746
BtgI CCRYGG 1 cut(s) 349
BtsCI GGATG 1 cut(s) 1005
BtsI GCAGTG 3 cut(s) 692, 773, 951
BtsIMutI CAGTG 5 cut(s) 420, 624, 692, 773, 951
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 3 cut(s) 13, 699, 735
CciI TCATGA 2 cut(s) 303, 576
CfoI GCGC 4 cut(s) 284, 359, 419, 862
Cfr13I GGNCC 2 cut(s) 363, 744
CseI GACGC 2 cut(s) 31, 53
Csp6I GTAC 2 cut(s) 310, 996
CviAII CATG 9 cut(s) 97, 304, 430, 482, 520, 577, 641, 857, 1053
CviQI GTAC 2 cut(s) 310, 996
DdeI CTNAG 5 cut(s) 384, 556, 754, 935, 989
DpnI GATC 5 cut(s) 83, 122, 302, 666, 1128
DpnII GATC 5 cut(s) 81, 120, 300, 664, 1126
DraIII CACNNNGTG 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
EciI GGCGGA 3 cut(s) 124, 157, 214
Ecl136II GAGCTC 1 cut(s) 53
Eco105I TACGTA 1 cut(s) 999
Eco24I GRGCYC 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 363
Eco53kI GAGCTC 1 cut(s) 53
Eco57I CTGAAG 1 cut(s) 1115
EcoICRI GAGCTC 1 cut(s) 53
EcoO109I RGGNCCY 1 cut(s) 744
EcoRII CCWGG 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 55
Esp3I CGTCTC 1 cut(s) 387
FaeI CATG 9 cut(s) 100, 307, 433, 485, 523, 580, 644, 860, 1056
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FaqI GGGAC 1 cut(s) 328
FatI CATG 9 cut(s) 96, 303, 429, 481, 519, 576, 640, 856, 1052
FauNDI CATATG 1 cut(s) 107
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 5 cut(s) 171, 280, 319, 355, 738
FokI GGATG 1 cut(s) 992
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 5 cut(s) 171, 280, 319, 355, 738
FspAI RTGCGCAY 1 cut(s) 861
FspBI CTAG 2 cut(s) 741, 881
FspI TGCGCA 1 cut(s) 861
GlaI GCGC 4 cut(s) 283, 358, 418, 861
GluI GCNGC 5 cut(s) 171, 280, 319, 355, 738
GsaI CCCAGC 1 cut(s) 93
GsuI CTGGAG 2 cut(s) 89, 257
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 1 cut(s) 746
HapII CCGG 3 cut(s) 31, 48, 78
HgaI GACGC 2 cut(s) 31, 53
HhaI GCGC 4 cut(s) 284, 359, 419, 862
Hin1I GRCGYC 2 cut(s) 23, 45
Hin1II CATG 9 cut(s) 100, 307, 433, 485, 523, 580, 644, 860, 1056
Hin6I GCGC 4 cut(s) 282, 357, 417, 860
HinP1I GCGC 4 cut(s) 282, 357, 417, 860
HincII GTYRAC 1 cut(s) 624
HindII GTYRAC 1 cut(s) 624
HinfI GANTC 6 cut(s) 233, 293, 568, 612, 770, 985
HpaII CCGG 3 cut(s) 31, 48, 78
HphI GGTGA 3 cut(s) 130, 241, 1081
Hpy166II GTNNAC 5 cut(s) 220, 366, 624, 841, 1072
Hpy188I TCNGA 8 cut(s) 57, 64, 241, 726, 936, 981, 1010, 1139
Hpy188III TCNNGA 4 cut(s) 274, 304, 577, 1037
Hpy8I GTNNAC 5 cut(s) 220, 366, 624, 841, 1072
Hpy99I CGWCG 2 cut(s) 25, 47
HpyCH4III ACNGT 5 cut(s) 537, 619, 628, 797, 1018
HpyCH4IV ACGT 2 cut(s) 410, 998
HpyCH4V TGCA 5 cut(s) 4, 408, 697, 737, 778
HpyF10VI GCNNNNNNNGC 2 cut(s) 414, 743
HpyF3I CTNAG 5 cut(s) 384, 556, 754, 935, 989
HpySE526I ACGT 2 cut(s) 410, 998
Hsp92I GRCGYC 2 cut(s) 23, 45
Hsp92II CATG 9 cut(s) 100, 307, 433, 485, 523, 580, 644, 860, 1056
HspAI GCGC 4 cut(s) 282, 357, 417, 860
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 5 cut(s) 81, 120, 300, 664, 1126
LmnI GCTCC 6 cut(s) 50, 264, 276, 426, 469, 1049
Lsp1109I GCAGC 2 cut(s) 330, 749
LweI GCATC 1 cut(s) 1014
MaeI CTAG 2 cut(s) 741, 881
MaeII ACGT 2 cut(s) 410, 998
MaeIII GTNAC 1 cut(s) 1094
MalI GATC 5 cut(s) 83, 122, 302, 666, 1128
MbiI CCGCTC 1 cut(s) 279
MboI GATC 5 cut(s) 81, 120, 300, 664, 1126
MboII GAAGA 7 cut(s) 173, 860, 863, 971, 994, 1121, 1136
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 1 cut(s) 1126
MhlI GDGCHC 2 cut(s) 13, 55
MlyI GAGTC 2 cut(s) 227, 302
MmeI TCCRAC 1 cut(s) 264
MroXI GAANNNNTTC 3 cut(s) 567, 809, 1128
MseI TTAA 4 cut(s) 438, 903, 908, 918
MslI CAYNNNNRTG 1 cut(s) 524
MspA1I CMGCKG 1 cut(s) 598
MspI CCGG 3 cut(s) 31, 48, 78
MspR9I CCNGG 2 cut(s) 79, 672
MunI CAATTG 1 cut(s) 924
MvaI CCWGG 1 cut(s) 672
MwoI GCNNNNNNNGC 2 cut(s) 414, 743
NciI CCSGG 1 cut(s) 79
NdeI CATATG 1 cut(s) 107
NdeII GATC 5 cut(s) 81, 120, 300, 664, 1126
NlaIII CATG 9 cut(s) 100, 307, 433, 485, 523, 580, 644, 860, 1056
NlaIV GGNNCC 1 cut(s) 534
NmuCI GTSAC 1 cut(s) 1094
NsbI TGCGCA 1 cut(s) 861
NspI RCATGY 2 cut(s) 860, 1056
PagI TCATGA 2 cut(s) 303, 576
PaqCI CACCTGC 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 840
PdmI GAANNNNTTC 3 cut(s) 567, 809, 1128
PfeI GAWTC 4 cut(s) 568, 612, 770, 985
PfoI TCCNGGA 1 cut(s) 77
PkrI GCNGC 5 cut(s) 172, 281, 320, 356, 739
PleI GAGTC 2 cut(s) 227, 301
PpsI GAGTC 2 cut(s) 227, 301
Ppu21I YACGTR 1 cut(s) 999
PshBI ATTAAT 1 cut(s) 903
Psp124BI GAGCTC 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 670
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 1 cut(s) 534
PspPI GGNCC 2 cut(s) 363, 744
PstI CTGCAG 1 cut(s) 739
PsuI RGATCY 1 cut(s) 1126
PvuII CAGCTG 1 cut(s) 598
RsaI GTAC 2 cut(s) 311, 997
RsaNI GTAC 2 cut(s) 310, 996
RseI CAYNNNNRTG 1 cut(s) 524
SacI GAGCTC 1 cut(s) 55
SaqAI TTAA 4 cut(s) 438, 903, 908, 918
SatI GCNGC 5 cut(s) 171, 280, 319, 355, 738
Sau3AI GATC 5 cut(s) 81, 120, 300, 664, 1126
Sau96I GGNCC 2 cut(s) 363, 744
SchI GAGTC 2 cut(s) 227, 302
ScrFI CCNGG 2 cut(s) 79, 672
SduI GDGCHC 2 cut(s) 13, 55
SfaNI GCATC 1 cut(s) 1014
SfcI CTRYAG 1 cut(s) 735
SinI GGWCC 1 cut(s) 363
SmiMI CAYNNNNRTG 1 cut(s) 524
SnaBI TACGTA 1 cut(s) 999
SsiI CCGC 6 cut(s) 135, 168, 171, 225, 279, 354
SspMI CTAG 2 cut(s) 741, 881
SstI GAGCTC 1 cut(s) 55
StyD4I CCNGG 2 cut(s) 77, 670
TaaI ACNGT 5 cut(s) 537, 619, 628, 797, 1018
TaiI ACGT 2 cut(s) 413, 1001
TaqI TCGA 2 cut(s) 42, 144
TauI GCSGC 3 cut(s) 173, 282, 357
TfiI GAWTC 4 cut(s) 568, 612, 770, 985
Tru1I TTAA 4 cut(s) 438, 903, 908, 918
Tru9I TTAA 4 cut(s) 438, 903, 908, 918
TscAI CASTG 5 cut(s) 427, 624, 699, 780, 958
TseFI GTSAC 1 cut(s) 1094
TseI GCWGC 2 cut(s) 318, 737
Tsp45I GTSAC 1 cut(s) 1094
TspDTI ATGAA 6 cut(s) 85, 470, 508, 629, 802, 861
TspGWI ACGGA 3 cut(s) 205, 286, 849
TspRI CASTG 5 cut(s) 427, 624, 699, 780, 958
VpaK11BI GGWCC 1 cut(s) 363
VspI ATTAAT 1 cut(s) 903
XapI RAATTY 5 cut(s) 194, 679, 714, 807, 941
XceI RCATGY 2 cut(s) 860, 1056
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XmnI GAANNNNTTC 3 cut(s) 567, 809, 1128
XspI CTAG 2 cut(s) 741, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.