FvH4_2g04300
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007961
Physical Location & Seq
Forward (+)
3353473 .. 3356118
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04300.t1

Sequence Viewer

Length: 837 bp
ATGAGGTACGATAGCAGCTTGGGGAAGCACAATGGCAAGTCCCACCACGGCGGCTCAAGGTCCACCTTGTGCGTTTCGTCTCAGGTTGGTTGTAAAATGGGGTGTACTTTCTGTGCCACTGGGACTATGGGGTTTAAGAGCAATTTGACCTCTGGGGAGATTGTGGAGCAGTTGGTTCATGCGTCCCGAATTTCGCCTATACGCAATGTTGTTTTCATGGGAATGGGAGAACCGTTGAATAACTATAATGCATTAGTGGAAGCGATTCGTGTCATGACTGGTTTACCATTTCAGTTGTCGCCAAAGAGGATTACTGTGTCAACGGTTGGTATTATCCATGCTATCAACAAGTTCCATAATGATCTACCTGGACTGAATTTAGCAGTTTCACTGCATGCACCTGTCCAAGAAATTCGTTGTCAGATAATGCCTGCAGCTCGGGCCTTTCCCTTAGTAAAGCTTATGGATTCATTGCAACAATATCAAAAGAACAGTCAGCAAAAAATTTTCATTGAATACATAATGCTTGATGGAGTGAATGATGAAGAGGAACACGCACACCAGCTTGGAAAATTGTTAGATACGTTTCAAGTGGTTATTAATTTAATACCCTTTAACCCAATTGGTAGTCTGAGCAAATTCAAAACCACTGCCGAAGAGAAAGTATCTACTTTTCAGAAGATTCTAAGAGGTACATATGGCATCCGAACAACTGTTCGCAAGGAAATGGGGCAGGACATAAGTGGAGCATGTGGTCAGTTGGTGGTGAACCCCAATCAGAGGTCAAGTGACAACGCAGTTCCTCCAACTGATATAGAAGATCTTCAGGTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

279

Amino Acids

30.61

Weight (kDa)

8.88

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 26 - 186 9.6e-12 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 51
AcsI RAATTY 5 cut(s) 189, 376, 411, 504, 638
AcuI CTGAAG 1 cut(s) 809
AdeI CACNNNGTG 1 cut(s) 69
AfaI GTAC 3 cut(s) 8, 106, 694
AfiI CCNNNNNNNGG 1 cut(s) 780
AgsI TTSAA 4 cut(s) 238, 515, 590, 643
AjnI CCWGG 1 cut(s) 367
AluBI AGCT 4 cut(s) 18, 437, 460, 565
AluI AGCT 4 cut(s) 18, 437, 460, 565
Alw26I GTCTC 1 cut(s) 84
Ama87I CYCGRG 1 cut(s) 438
AoxI GGCC 1 cut(s) 441
ApeKI GCWGC 2 cut(s) 15, 434
ApoI RAATTY 5 cut(s) 189, 376, 411, 504, 638
ArsI GACNNNNNNTTYG 2 cut(s) 478, 510
AseI ATTAAT 1 cut(s) 600
Asp700I GAANNNNTTC 2 cut(s) 264, 822
AspS9I GGNCC 2 cut(s) 60, 441
AsuHPI GGTGA 1 cut(s) 778
AvaI CYCGRG 1 cut(s) 438
AvaII GGWCC 1 cut(s) 60
BbvI GCAGC 2 cut(s) 27, 446
BccI CCATC 1 cut(s) 524
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 369
BcoDI GTCTC 1 cut(s) 84
BfmI CTRYAG 1 cut(s) 432
BglII AGATCT 1 cut(s) 820
BisI GCNGC 3 cut(s) 16, 52, 435
BlsI GCNGC 3 cut(s) 17, 53, 436
Bme1390I CCNGG 1 cut(s) 369
Bme18I GGWCC 1 cut(s) 60
BmeT110I CYCGRG 1 cut(s) 438
BmgT120I GGNCC 2 cut(s) 60, 441
BmrFI CCNGG 1 cut(s) 369
BmrI ACTGGG 1 cut(s) 129
BmsI GCATC 1 cut(s) 711
BmuI ACTGGG 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 40
BsaJI CCNNGG 1 cut(s) 46
Bsc4I CCNNNNNNNGG 1 cut(s) 780
Bse1I ACTGG 2 cut(s) 124, 283
Bse3DI GCAATG 2 cut(s) 211, 470
BseBI CCWGG 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 46
BseGI GGATG 1 cut(s) 702
BseLI CCNNNNNNNGG 1 cut(s) 780
BseMI GCAATG 2 cut(s) 211, 470
BseMII CTCAG 2 cut(s) 95, 623
BseNI ACTGG 2 cut(s) 124, 283
BseXI GCAGC 2 cut(s) 27, 446
BshFI GGCC 1 cut(s) 443
BsiHKCI CYCGRG 1 cut(s) 438
BslFI GGGAC 3 cut(s) 25, 136, 169
BslI CCNNNNNNNGG 1 cut(s) 780
BsmAI GTCTC 1 cut(s) 84
BsmBI CGTCTC 1 cut(s) 84
BsmFI GGGAC 3 cut(s) 25, 136, 169
BsnI GGCC 1 cut(s) 443
BsoBI CYCGRG 1 cut(s) 438
Bsp143I GATC 2 cut(s) 361, 820
BspACI CCGC 1 cut(s) 51
BspANI GGCC 1 cut(s) 443
BspCNI CTCAG 2 cut(s) 94, 624
BspHI TCATGA 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 436
BsrDI GCAATG 2 cut(s) 211, 470
BsrI ACTGG 2 cut(s) 124, 283
BssECI CCNNGG 1 cut(s) 46
BssMI GATC 2 cut(s) 361, 820
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 5 cut(s) 234, 316, 325, 494, 715
Bst6I CTCTTC 2 cut(s) 540, 651
BstC8I GCNNGC 2 cut(s) 396, 432
BstDEI CTNAG 4 cut(s) 81, 451, 632, 686
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 1 cut(s) 702
BstKTI GATC 2 cut(s) 364, 823
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 2 cut(s) 361, 820
BstMWI GCNNNNNNNGC 1 cut(s) 440
BstNI CCWGG 1 cut(s) 369
BstNSI RCATGY 2 cut(s) 398, 753
BstSCI CCNGG 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 432
BstV1I GCAGC 2 cut(s) 27, 446
BstX2I RGATCY 1 cut(s) 820
BstYI RGATCY 1 cut(s) 820
BsuRI GGCC 1 cut(s) 443
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 1 cut(s) 702
BtsI GCAGTG 2 cut(s) 389, 648
BtsIMutI CAGTG 3 cut(s) 117, 389, 648
Cac8I GCNNGC 2 cut(s) 396, 432
CciI TCATGA 1 cut(s) 273
Cfr13I GGNCC 2 cut(s) 60, 441
CseI GACGC 1 cut(s) 171
Csp6I GTAC 3 cut(s) 7, 105, 693
CviAII CATG 6 cut(s) 179, 217, 274, 338, 395, 750
CviJI RGCY 6 cut(s) 18, 54, 437, 443, 460, 565
CviKI_1 RGCY 6 cut(s) 18, 54, 437, 443, 460, 565
CviQI GTAC 3 cut(s) 7, 105, 693
DdeI CTNAG 4 cut(s) 81, 451, 632, 686
DpnI GATC 2 cut(s) 363, 822
DpnII GATC 2 cut(s) 361, 820
DraIII CACNNNGTG 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 540, 651
EarI CTCTTC 2 cut(s) 540, 651
Eco47I GGWCC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 809
Eco88I CYCGRG 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 367
EcoT22I ATGCAT 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 6 cut(s) 182, 220, 277, 341, 398, 753
FaqI GGGAC 3 cut(s) 25, 136, 169
FatI CATG 6 cut(s) 178, 216, 273, 337, 394, 749
FauNDI CATATG 1 cut(s) 697
Fnu4HI GCNGC 3 cut(s) 16, 52, 435
FokI GGATG 1 cut(s) 689
Fsp4HI GCNGC 3 cut(s) 16, 52, 435
GluI GCNGC 3 cut(s) 16, 52, 435
HaeIII GGCC 1 cut(s) 443
HgaI GACGC 1 cut(s) 171
Hin1II CATG 6 cut(s) 182, 220, 277, 341, 398, 753
HincII GTYRAC 1 cut(s) 321
HindII GTYRAC 1 cut(s) 321
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 3 cut(s) 265, 467, 682
HphI GGTGA 1 cut(s) 778
Hpy166II GTNNAC 5 cut(s) 63, 105, 284, 321, 769
Hpy188I TCNGA 6 cut(s) 423, 633, 678, 707, 780, 833
Hpy188III TCNNGA 2 cut(s) 186, 274
Hpy8I GTNNAC 5 cut(s) 63, 105, 284, 321, 769
HpyCH4III ACNGT 5 cut(s) 234, 316, 325, 494, 715
HpyCH4IV ACGT 1 cut(s) 584
HpyCH4V TGCA 5 cut(s) 251, 394, 398, 434, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 440
HpyF3I CTNAG 4 cut(s) 81, 451, 632, 686
HpySE526I ACGT 1 cut(s) 584
Hsp92II CATG 6 cut(s) 182, 220, 277, 341, 398, 753
Kzo9I GATC 2 cut(s) 361, 820
LmnI GCTCC 2 cut(s) 166, 746
Lsp1109I GCAGC 2 cut(s) 27, 446
LweI GCATC 1 cut(s) 711
MaeII ACGT 1 cut(s) 584
MaeIII GTNAC 1 cut(s) 788
MalI GATC 2 cut(s) 363, 822
MboI GATC 2 cut(s) 361, 820
MboII GAAGA 5 cut(s) 557, 668, 691, 815, 830
MfeI CAATTG 1 cut(s) 621
MflI RGATCY 1 cut(s) 820
MluCI AATT 9 cut(s) 142, 189, 376, 411, 504, 572, 601, 621, 638
MmeI TCCRAC 1 cut(s) 830
MnlI CCTC 6 cut(s) 160, 300, 541, 683, 774, 813
Mph1103I ATGCAT 1 cut(s) 253
MroXI GAANNNNTTC 2 cut(s) 264, 822
MseI TTAA 4 cut(s) 135, 600, 605, 615
MslI CAYNNNNRTG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 369
MunI CAATTG 1 cut(s) 621
MvaI CCWGG 1 cut(s) 369
MwoI GCNNNNNNNGC 1 cut(s) 440
NdeI CATATG 1 cut(s) 697
NdeII GATC 2 cut(s) 361, 820
NlaIII CATG 6 cut(s) 182, 220, 277, 341, 398, 753
NmuCI GTSAC 1 cut(s) 788
NsiI ATGCAT 1 cut(s) 253
NspI RCATGY 2 cut(s) 398, 753
PaeI GCATGC 1 cut(s) 398
PagI TCATGA 1 cut(s) 273
PdmI GAANNNNTTC 2 cut(s) 264, 822
PfeI GAWTC 3 cut(s) 265, 467, 682
PkrI GCNGC 3 cut(s) 17, 53, 436
PshBI ATTAAT 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 367
PspPI GGNCC 2 cut(s) 60, 441
PstI CTGCAG 1 cut(s) 436
PsuI RGATCY 1 cut(s) 820
RsaI GTAC 3 cut(s) 8, 106, 694
RsaNI GTAC 3 cut(s) 7, 105, 693
RseI CAYNNNNRTG 1 cut(s) 221
SaqAI TTAA 4 cut(s) 135, 600, 605, 615
SatI GCNGC 3 cut(s) 16, 52, 435
Sau3AI GATC 2 cut(s) 361, 820
Sau96I GGNCC 2 cut(s) 60, 441
ScrFI CCNGG 1 cut(s) 369
SfaNI GCATC 1 cut(s) 711
SfcI CTRYAG 1 cut(s) 432
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
SphI GCATGC 1 cut(s) 398
Sse9I AATT 9 cut(s) 142, 189, 376, 411, 504, 572, 601, 621, 638
SsiI CCGC 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 367
TaaI ACNGT 5 cut(s) 234, 316, 325, 494, 715
TaiI ACGT 1 cut(s) 587
TasI AATT 9 cut(s) 142, 189, 376, 411, 504, 572, 601, 621, 638
TatI WGTACW 1 cut(s) 104
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 3 cut(s) 265, 467, 682
Tru1I TTAA 4 cut(s) 135, 600, 605, 615
Tru9I TTAA 4 cut(s) 135, 600, 605, 615
TscAI CASTG 3 cut(s) 124, 396, 655
TseFI GTSAC 1 cut(s) 788
TseI GCWGC 2 cut(s) 15, 434
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 5 cut(s) 167, 205, 459, 499, 558
TspRI CASTG 3 cut(s) 124, 396, 655
VpaK11BI GGWCC 1 cut(s) 60
VspI ATTAAT 1 cut(s) 600
XapI RAATTY 5 cut(s) 189, 376, 411, 504, 638
XceI RCATGY 2 cut(s) 398, 753
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XmnI GAANNNNTTC 2 cut(s) 264, 822
Zsp2I ATGCAT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.