Rw6G005470
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0007961
Physical Location & Seq
Forward (+)
8748681 .. 8751497
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G005470.1

Sequence Viewer

Length: 831 bp
ATGAGGTACGATAGCAGCTTGGGGAAGCACAATGGCAAGTCCCACCACGGCGGCGCAAGGTCCACCTTGTGCGTATCGTCTCAGGTTGGTTGTAAAATGGGTTGCACGTTCTGTGCCACTGGGAGTATGGGGTTTAAGAGCAATTTGACCTCTGGGGAGATTGTGGAGCAGTTGGTTCATGCTTCTCGCATTTCGCCTATACGCAATGTTGTTTTCATGGGAATGGGGGAACCGTTGAATAACTATAATGCCTTAGTGGAAGCAATTCATGTCATGACTGGATTACCATTTCAGCTGTCGCCAAAGAGAATCACAGTGTCAACGGTTGGTATTATTCATGCTATCAACAAGTTCCATAATGATCTCCCTGGACTGAATTTAGCAGTTTCACTGCATGCACCTGTCCAAGAAATTCGTTGTCAGATAATGCCTGCAGCTCGGGCCTTTCCCTTAGTAAAACTTATGGATTCACTGCAACAATATCAAAAGAACAGTCAGCAGAAAATTTTCATTGAATACATAATGCTTGACGGAGTGAACGATGAAGAAGAACATGCGCACCAGCTTGGGAAATTGCTAGATACATTTCAAGTGGTAATTAATTTAATACCTTTTAACCCAATTGGTAGTCTGAGCAAATTCAAAACCACTGCCGAAGAAAAAGTATCTGTTTTTCAGAAGATTCTAAGAGGTACGTATGGCATCCGAACAACCGTTCGCAAGGAAATGGGTCAGGACATAAGTGGAGCGTGTGGTCAGTTGGTGGTGAACATACCCAATAAGAGGTCAAGTGACCCTCTAACTGATATAGAAGATCTTCAGGTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

276

Amino Acids

30.4

Weight (kDa)

8.87

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 26 - 186 1.5e-11 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 558
AciI CCGC 1 cut(s) 51
AcsI RAATTY 4 cut(s) 376, 411, 504, 638
AcuI CTGAAG 1 cut(s) 803
AdeI CACNNNGTG 1 cut(s) 69
AfaI GTAC 2 cut(s) 8, 694
AfiI CCNNNNNNNGG 1 cut(s) 783
AgsI TTSAA 4 cut(s) 238, 515, 590, 643
AjnI CCWGG 1 cut(s) 367
AluBI AGCT 4 cut(s) 18, 295, 437, 565
AluI AGCT 4 cut(s) 18, 295, 437, 565
Alw26I GTCTC 1 cut(s) 84
Ama87I CYCGRG 1 cut(s) 438
AoxI GGCC 1 cut(s) 441
ApeKI GCWGC 2 cut(s) 15, 434
ApoI RAATTY 4 cut(s) 376, 411, 504, 638
ArsI GACNNNNNNTTYG 2 cut(s) 478, 510
AseI ATTAAT 1 cut(s) 600
Asp700I GAANNNNTTC 3 cut(s) 264, 506, 816
AspLEI GCGC 2 cut(s) 56, 559
AspS9I GGNCC 2 cut(s) 60, 441
AsuHPI GGTGA 1 cut(s) 778
AvaI CYCGRG 1 cut(s) 438
AvaII GGWCC 1 cut(s) 60
BbvI GCAGC 2 cut(s) 27, 446
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 369
BcoDI GTCTC 1 cut(s) 84
BfaI CTAG 1 cut(s) 578
BfmI CTRYAG 1 cut(s) 432
BglII AGATCT 1 cut(s) 814
BisI GCNGC 3 cut(s) 16, 52, 435
BlsI GCNGC 3 cut(s) 17, 53, 436
Bme1390I CCNGG 1 cut(s) 369
Bme18I GGWCC 1 cut(s) 60
BmeT110I CYCGRG 1 cut(s) 438
BmgT120I GGNCC 2 cut(s) 60, 441
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 1 cut(s) 369
BmrI ACTGGG 1 cut(s) 129
BmsI GCATC 1 cut(s) 711
BmuI ACTGGG 1 cut(s) 129
BsaAI YACGTR 1 cut(s) 696
BsaJI CCNNGG 2 cut(s) 46, 367
Bsc4I CCNNNNNNNGG 1 cut(s) 783
Bse1I ACTGG 2 cut(s) 124, 283
Bse3DI GCAATG 1 cut(s) 211
BseBI CCWGG 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 46, 367
BseGI GGATG 1 cut(s) 702
BseLI CCNNNNNNNGG 1 cut(s) 783
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 95, 623
BseNI ACTGG 2 cut(s) 124, 283
BseXI GCAGC 2 cut(s) 27, 446
BshFI GGCC 1 cut(s) 443
BsiHKCI CYCGRG 1 cut(s) 438
BslFI GGGAC 1 cut(s) 25
BslI CCNNNNNNNGG 1 cut(s) 783
BsmAI GTCTC 1 cut(s) 84
BsmBI CGTCTC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 25
BsnI GGCC 1 cut(s) 443
BsoBI CYCGRG 1 cut(s) 438
Bsp143I GATC 2 cut(s) 361, 814
BspACI CCGC 1 cut(s) 51
BspANI GGCC 1 cut(s) 443
BspCNI CTCAG 2 cut(s) 94, 624
BspHI TCATGA 1 cut(s) 273
BspLI GGNNCC 1 cut(s) 231
BspMAI CTGCAG 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 211
BsrI ACTGG 2 cut(s) 124, 283
BssECI CCNNGG 2 cut(s) 46, 367
BssMI GATC 2 cut(s) 361, 814
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 5 cut(s) 234, 316, 325, 494, 715
BstBAI YACGTR 1 cut(s) 696
BstC8I GCNNGC 2 cut(s) 396, 432
BstDEI CTNAG 5 cut(s) 81, 253, 451, 632, 686
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 1 cut(s) 702
BstHHI GCGC 2 cut(s) 56, 559
BstKTI GATC 2 cut(s) 364, 817
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 2 cut(s) 361, 814
BstMWI GCNNNNNNNGC 1 cut(s) 440
BstNI CCWGG 1 cut(s) 369
BstNSI RCATGY 2 cut(s) 398, 557
BstSCI CCNGG 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 432
BstSNI TACGTA 1 cut(s) 696
BstV1I GCAGC 2 cut(s) 27, 446
BstX2I RGATCY 1 cut(s) 814
BstYI RGATCY 1 cut(s) 814
BsuRI GGCC 1 cut(s) 443
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 1 cut(s) 702
BtsI GCAGTG 3 cut(s) 389, 470, 648
BtsIMutI CAGTG 5 cut(s) 117, 321, 389, 470, 648
Cac8I GCNNGC 2 cut(s) 396, 432
CciI TCATGA 1 cut(s) 273
CfoI GCGC 2 cut(s) 56, 559
Cfr13I GGNCC 2 cut(s) 60, 441
Csp6I GTAC 2 cut(s) 7, 693
CviAII CATG 7 cut(s) 179, 217, 269, 274, 338, 395, 554
CviJI RGCY 5 cut(s) 18, 295, 437, 443, 565
CviKI_1 RGCY 5 cut(s) 18, 295, 437, 443, 565
CviQI GTAC 2 cut(s) 7, 693
DdeI CTNAG 5 cut(s) 81, 253, 451, 632, 686
DpnI GATC 2 cut(s) 363, 816
DpnII GATC 2 cut(s) 361, 814
DraIII CACNNNGTG 1 cut(s) 69
Eco105I TACGTA 1 cut(s) 696
Eco47I GGWCC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 803
Eco88I CYCGRG 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 367
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 7 cut(s) 182, 220, 272, 277, 341, 398, 557
FaqI GGGAC 1 cut(s) 25
FatI CATG 7 cut(s) 178, 216, 268, 273, 337, 394, 553
Fnu4HI GCNGC 3 cut(s) 16, 52, 435
FokI GGATG 1 cut(s) 689
Fsp4HI GCNGC 3 cut(s) 16, 52, 435
FspAI RTGCGCAY 1 cut(s) 558
FspBI CTAG 1 cut(s) 578
FspI TGCGCA 1 cut(s) 558
GlaI GCGC 2 cut(s) 55, 558
GluI GCNGC 3 cut(s) 16, 52, 435
HaeIII GGCC 1 cut(s) 443
HhaI GCGC 2 cut(s) 56, 559
Hin1II CATG 7 cut(s) 182, 220, 272, 277, 341, 398, 557
Hin6I GCGC 2 cut(s) 54, 557
HinP1I GCGC 2 cut(s) 54, 557
HincII GTYRAC 1 cut(s) 321
HindII GTYRAC 1 cut(s) 321
HinfI GANTC 3 cut(s) 309, 467, 682
HphI GGTGA 1 cut(s) 778
Hpy166II GTNNAC 4 cut(s) 63, 321, 538, 769
Hpy188I TCNGA 5 cut(s) 423, 633, 678, 707, 827
Hpy188III TCNNGA 2 cut(s) 274, 734
Hpy8I GTNNAC 4 cut(s) 63, 321, 538, 769
HpyCH4III ACNGT 5 cut(s) 234, 316, 325, 494, 715
HpyCH4IV ACGT 2 cut(s) 107, 695
HpyCH4V TGCA 5 cut(s) 105, 394, 398, 434, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 440
HpyF3I CTNAG 5 cut(s) 81, 253, 451, 632, 686
HpySE526I ACGT 2 cut(s) 107, 695
Hsp92II CATG 7 cut(s) 182, 220, 272, 277, 341, 398, 557
HspAI GCGC 2 cut(s) 54, 557
Kzo9I GATC 2 cut(s) 361, 814
LmnI GCTCC 2 cut(s) 166, 746
Lsp1109I GCAGC 2 cut(s) 27, 446
LweI GCATC 1 cut(s) 711
MaeI CTAG 1 cut(s) 578
MaeII ACGT 2 cut(s) 107, 695
MaeIII GTNAC 1 cut(s) 791
MalI GATC 2 cut(s) 363, 816
MboI GATC 2 cut(s) 361, 814
MboII GAAGA 6 cut(s) 557, 560, 668, 691, 809, 824
MfeI CAATTG 1 cut(s) 621
MflI RGATCY 1 cut(s) 814
MnlI CCTC 4 cut(s) 160, 683, 777, 807
MroXI GAANNNNTTC 3 cut(s) 264, 506, 816
MseI TTAA 4 cut(s) 135, 600, 605, 615
MslI CAYNNNNRTG 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 295
MspR9I CCNGG 1 cut(s) 369
MunI CAATTG 1 cut(s) 621
MvaI CCWGG 1 cut(s) 369
MwoI GCNNNNNNNGC 1 cut(s) 440
NdeII GATC 2 cut(s) 361, 814
NlaIII CATG 7 cut(s) 182, 220, 272, 277, 341, 398, 557
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 791
NsbI TGCGCA 1 cut(s) 558
NspI RCATGY 2 cut(s) 398, 557
PaeI GCATGC 1 cut(s) 398
PagI TCATGA 1 cut(s) 273
PcsI WCGNNNNNNNCGW 1 cut(s) 537
PdmI GAANNNNTTC 3 cut(s) 264, 506, 816
PfeI GAWTC 3 cut(s) 309, 467, 682
PkrI GCNGC 3 cut(s) 17, 53, 436
Ppu21I YACGTR 1 cut(s) 696
PshBI ATTAAT 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 367
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 2 cut(s) 60, 441
PstI CTGCAG 1 cut(s) 436
PsuI RGATCY 1 cut(s) 814
PvuII CAGCTG 1 cut(s) 295
RsaI GTAC 2 cut(s) 8, 694
RsaNI GTAC 2 cut(s) 7, 693
RseI CAYNNNNRTG 1 cut(s) 221
SaqAI TTAA 4 cut(s) 135, 600, 605, 615
SatI GCNGC 3 cut(s) 16, 52, 435
Sau3AI GATC 2 cut(s) 361, 814
Sau96I GGNCC 2 cut(s) 60, 441
ScrFI CCNGG 1 cut(s) 369
SfaNI GCATC 1 cut(s) 711
SfcI CTRYAG 1 cut(s) 432
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 221
SnaBI TACGTA 1 cut(s) 696
SphI GCATGC 1 cut(s) 398
SsiI CCGC 1 cut(s) 51
SspMI CTAG 1 cut(s) 578
StyD4I CCNGG 1 cut(s) 367
TaaI ACNGT 5 cut(s) 234, 316, 325, 494, 715
TaiI ACGT 2 cut(s) 110, 698
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 3 cut(s) 309, 467, 682
Tru1I TTAA 4 cut(s) 135, 600, 605, 615
Tru9I TTAA 4 cut(s) 135, 600, 605, 615
TscAI CASTG 5 cut(s) 124, 321, 396, 477, 655
TseFI GTSAC 1 cut(s) 791
TseI GCWGC 2 cut(s) 15, 434
Tsp45I GTSAC 1 cut(s) 791
TspDTI ATGAA 6 cut(s) 167, 205, 257, 326, 499, 558
TspGWI ACGGA 1 cut(s) 546
TspRI CASTG 5 cut(s) 124, 321, 396, 477, 655
VpaK11BI GGWCC 1 cut(s) 60
VspI ATTAAT 1 cut(s) 600
XapI RAATTY 4 cut(s) 376, 411, 504, 638
XceI RCATGY 2 cut(s) 398, 557
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XmnI GAANNNNTTC 3 cut(s) 264, 506, 816
XspI CTAG 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.