MD05G1099600.v1.1
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007961
Physical Location & Seq
Forward (+)
20715806 .. 20716692
887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1099600.v1.1.491

Sequence Viewer

Length: 417 bp
ATGGACTCCTCAGACCAACTCACCACCAAGCTCCTCATAAAACTGCAGAATGGGGCTCTTGTGGAGGCTGTGATTATGCGATATGACAGCCGCTTGGGAAGGTATGACGGGAAGCCCCGCCCTGGTGGTCCTAGGTCCACCTTGTGTGTTGGTTGCAAAATGGGTTGTACTTTCTGTGCTACCGAGAGTATGGGGTTTAAGAGCAATCTGACATCGGGGGAGATTGTGGAGCAATTGGTTCATGCTTCTCGCATTTCGCCTATACGCAATATTGTGTTCATGGGAATGGGAGAACAGTTGAATAACTATGGTTCCTTAGTGGAAGCGATTCAAGTCATGACTGGATCACCATTTCATCTGTCACGAAAGAAAATTACTGTGTCGACGGTGGGCATCGAAAATTACCATTATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

139

Amino Acids

15.15

Weight (kDa)

8.98

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 49 - 132 1.3e-06 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 383
AciI CCGC 2 cut(s) 91, 118
AclWI GGATC 1 cut(s) 352
AdeI CACNNNGTG 1 cut(s) 144
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 122
AgsI TTSAA 2 cut(s) 301, 332
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 1 cut(s) 31
AluI AGCT 1 cut(s) 31
AlwI GGATC 1 cut(s) 352
Asp700I GAANNNNTTC 1 cut(s) 327
AspA2I CCTAGG 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 128, 135
AsuHPI GGTGA 2 cut(s) 13, 339
AvaII GGWCC 2 cut(s) 128, 135
AvrII CCTAGG 1 cut(s) 131
BanII GRGCYC 1 cut(s) 58
BciT130I CCWGG 1 cut(s) 123
BfaI CTAG 1 cut(s) 132
BfmI CTRYAG 1 cut(s) 44
BisI GCNGC 1 cut(s) 91
BlnI CCTAGG 1 cut(s) 131
BlsI GCNGC 1 cut(s) 92
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 2 cut(s) 128, 135
BmgT120I GGNCC 2 cut(s) 128, 135
BmiI GGNNCC 1 cut(s) 313
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 1 cut(s) 402
BsaJI CCNNGG 2 cut(s) 121, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 346
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 2 cut(s) 121, 131
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 346
BseRI GAGGAG 1 cut(s) 23
BslI CCNNNNNNNGG 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 58
Bsp143I GATC 1 cut(s) 344
BspACI CCGC 2 cut(s) 91, 118
BspCNI CTCAG 1 cut(s) 23
BspHI TCATGA 1 cut(s) 336
BspLI GGNNCC 1 cut(s) 313
BspMAI CTGCAG 1 cut(s) 48
BspPI GGATC 1 cut(s) 352
BsrI ACTGG 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 121, 131
BssMI GATC 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 131
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 3 cut(s) 297, 379, 388
BstDEI CTNAG 2 cut(s) 10, 316
BstKTI GATC 1 cut(s) 347
BstMBI GATC 1 cut(s) 344
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 1 cut(s) 44
CciI TCATGA 1 cut(s) 336
Cfr13I GGNCC 2 cut(s) 128, 135
Csp6I GTAC 1 cut(s) 168
CviAII CATG 3 cut(s) 242, 280, 337
CviJI RGCY 5 cut(s) 31, 56, 68, 90, 115
CviKI_1 RGCY 5 cut(s) 31, 56, 68, 90, 115
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 2 cut(s) 10, 316
DpnI GATC 1 cut(s) 346
DpnII GATC 1 cut(s) 344
DraIII CACNNNGTG 1 cut(s) 144
Eco130I CCWWGG 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 58
Eco47I GGWCC 2 cut(s) 128, 135
EcoRII CCWGG 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 131
EcoT38I GRGCYC 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 131
FaeI CATG 3 cut(s) 245, 283, 340
FatI CATG 3 cut(s) 241, 279, 336
FauI CCCGC 1 cut(s) 125
FblI GTMKAC 1 cut(s) 383
Fnu4HI GCNGC 1 cut(s) 91
FriOI GRGCYC 1 cut(s) 58
Fsp4HI GCNGC 1 cut(s) 91
FspBI CTAG 1 cut(s) 132
GluI GCNGC 1 cut(s) 91
Hin1II CATG 3 cut(s) 245, 283, 340
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HinfI GANTC 2 cut(s) 5, 328
HphI GGTGA 2 cut(s) 13, 339
Hpy166II GTNNAC 2 cut(s) 138, 384
Hpy188I TCNGA 2 cut(s) 13, 210
Hpy188III TCNNGA 2 cut(s) 337, 363
Hpy8I GTNNAC 2 cut(s) 138, 384
Hpy99I CGWCG 1 cut(s) 388
HpyAV CCTTC 1 cut(s) 93
HpyCH4III ACNGT 3 cut(s) 297, 379, 388
HpyCH4V TGCA 2 cut(s) 46, 156
HpyF3I CTNAG 2 cut(s) 10, 316
Hsp92II CATG 3 cut(s) 245, 283, 340
Kzo9I GATC 1 cut(s) 344
LmnI GCTCC 2 cut(s) 36, 229
LpnPI CCDG 3 cut(s) 108, 135, 327
LweI GCATC 1 cut(s) 402
MaeI CTAG 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 360
MalI GATC 1 cut(s) 346
MboI GATC 1 cut(s) 344
MfeI CAATTG 1 cut(s) 233
MhlI GDGCHC 1 cut(s) 58
MluCI AATT 3 cut(s) 233, 372, 400
MnlI CCTC 3 cut(s) 19, 44, 58
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 123
MunI CAATTG 1 cut(s) 233
MvaI CCWGG 1 cut(s) 123
NdeII GATC 1 cut(s) 344
NlaIII CATG 3 cut(s) 245, 283, 340
NlaIV GGNNCC 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 360
PagI TCATGA 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 328
PkrI GCNGC 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 1 cut(s) 313
PspPI GGNCC 2 cut(s) 128, 135
PstI CTGCAG 1 cut(s) 48
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
RseI CAYNNNNRTG 1 cut(s) 284
SalI GTCGAC 1 cut(s) 382
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 1 cut(s) 91
Sau3AI GATC 1 cut(s) 344
Sau96I GGNCC 2 cut(s) 128, 135
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 58
SetI ASST 4 cut(s) 33, 104, 137, 143
SfaNI GCATC 1 cut(s) 402
SfcI CTRYAG 1 cut(s) 44
SinI GGWCC 2 cut(s) 128, 135
SmiMI CAYNNNNRTG 1 cut(s) 284
Sse9I AATT 3 cut(s) 233, 372, 400
SsiI CCGC 2 cut(s) 91, 118
SspI AATATT 1 cut(s) 271
SspMI CTAG 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 1 cut(s) 131
TaaI ACNGT 3 cut(s) 297, 379, 388
TaqI TCGA 2 cut(s) 383, 396
TasI AATT 3 cut(s) 233, 372, 400
TatI WGTACW 1 cut(s) 167
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TseFI GTSAC 1 cut(s) 360
Tsp45I GTSAC 1 cut(s) 360
TspDTI ATGAA 3 cut(s) 230, 268, 344
VpaK11BI GGWCC 2 cut(s) 128, 135
XmaJI CCTAGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 383
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.