pycom05g09850
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007961
Physical Location & Seq
Forward (+)
13133402 .. 13135420
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g09850.1

Sequence Viewer

Length: 1209 bp
ATGATTTCCCAACAGAGCCATAAGCCACACTCCGATTCCGTTGCGGCAACACCCAAACCAGCATTCAAAACTATGCAAAGCGCGCGAAATACGACGGCGTTTCGATCAATCTTCGACGCCGGAGAGCTGAAATCGGAATTGGAAAATTCCGGGGTCAAGCCCAGCTTGATCCCCTACATATGGAAGCGGGTGATCCAAAACCCCGATGCCCAATTCGACCAAATTCCATCTTTGCCCTCGGCAGCGTACCCTCTCCTGACCTCCAAATTCAAAACCCTAACCTCCTCCGTCCACAAGGTCATGGACTCCTCAGACCAACTCACCACCAAGCTCCTCATAAAACTCCAGAATGGGGCTCTTGTGGAGGCTGTGATTATGCGATATGACAGCCGCTTGGGAAAGTATGACGGGAAGCCCCGCCCTGGTGGTCCTAGGTCCACCTTGTGTGTATCATCTCAGGTTGGTTGCAAAATGGGTTGTACTTTCTGTGCTACCGGGAGTATGGGGTTTAAGAGCAATCTGACATCGGGGGAGATTGTGGAGCAATTGGTTCATGCTTCTCGCATTTCGCCTATACGCAATATTGTGTTCATGGGAATGGGAGAACCGTTGAATAACTATGGTTCCTTAGTGGAAGCGATTCATGTCATGACTGGATCGCCATTTCACTTGTCGCCAAAGAAAATTACTGTGTCGACGGTCGGCATTATTCATGCTATCAAAAAGCTTCATAGTGATCTTCCAGGTCTGAACTTAGCAGTTTCACTTCATGCTCCAGTCCAAGAAATTCGTTGTCAGATAATGCCTGCGGCTCGGGCTTTTCCTTTAGTAAAGCTTATGGATACACTGCAAGAATATCAAAAGAGCACTCAGCAGAAAATCTTCATTGAGTACATAATGCTTGATGGAGTCAATGATGAAGAGCAACATGCACACCAACTCGGAAAATTGCTAGAGACATTTCAAGTGGTCATTAATTTAATACCTTTTAATCCAATTGGTATTCTGAGTAAATTCACAACCAGTAATGAAAACAAAGTAACGAACTTCCAGAAAATTCTACGAGGCACATACGGCATTCGAACAACAGTTCGCAAGGAAATGGGTCAAGATATAAGTGGAGCATGTGGTCAGTTGGTGGTGAACTTACCGAACAAGAGGTCAACTGAGAACACTCTAACTGATATAGAAGATATTCAGGTAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

403

Amino Acids

44.31

Weight (kDa)

9.3

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 151 - 309 2.6e-11 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 695
AccII CGCG 2 cut(s) 83, 85
AciI CCGC 5 cut(s) 44, 187, 391, 418, 809
AclWI GGATC 3 cut(s) 163, 187, 664
AcsI RAATTY 6 cut(s) 145, 222, 266, 786, 1013, 1056
AcyI GRCGYC 1 cut(s) 117
AdeI CACNNNGTG 1 cut(s) 444
AfaI GTAC 3 cut(s) 248, 481, 893
AfiI CCNNNNNNNGG 3 cut(s) 180, 352, 422
AgsI TTSAA 4 cut(s) 67, 271, 613, 965
AjnI CCWGG 2 cut(s) 421, 742
AjuI GAANNNNNNNTTGG 4 cut(s) 47, 79, 122, 154
AluBI AGCT 5 cut(s) 127, 165, 331, 727, 835
AluI AGCT 5 cut(s) 127, 165, 331, 727, 835
Alw21I GWGCWC 1 cut(s) 869
Alw26I GTCTC 1 cut(s) 950
AlwI GGATC 3 cut(s) 163, 187, 664
Ama87I CYCGRG 1 cut(s) 813
ApeKI GCWGC 1 cut(s) 242
ApoI RAATTY 6 cut(s) 145, 222, 266, 786, 1013, 1056
ArsI GACNNNNNNTTYG 2 cut(s) 1145, 1177
AseI ATTAAT 1 cut(s) 975
Asp700I GAANNNNTTC 3 cut(s) 639, 881, 1194
AspA2I CCTAGG 1 cut(s) 431
AspLEI GCGC 2 cut(s) 83, 85
AspS9I GGNCC 2 cut(s) 428, 435
AsuC2I CCSGG 2 cut(s) 151, 496
AsuHPI GGTGA 3 cut(s) 202, 313, 1153
AsuII TTCGAA 1 cut(s) 1081
AvaI CYCGRG 1 cut(s) 813
AvaII GGWCC 2 cut(s) 428, 435
AvrII CCTAGG 1 cut(s) 431
BanII GRGCYC 1 cut(s) 358
Bbv12I GWGCWC 1 cut(s) 869
BbvI GCAGC 1 cut(s) 254
BccI CCATC 2 cut(s) 235, 899
BceAI ACGGC 2 cut(s) 111, 1090
BcgI CGANNNNNNTGC 2 cut(s) 23, 57
BciT130I CCWGG 2 cut(s) 423, 744
BciVI GTATCC 1 cut(s) 835
BcnI CCSGG 2 cut(s) 151, 496
BcoDI GTCTC 1 cut(s) 950
BfaI CTAG 2 cut(s) 432, 953
BfuI GTATCC 1 cut(s) 835
BisI GCNGC 4 cut(s) 45, 243, 391, 810
BlnI CCTAGG 1 cut(s) 431
BlsI GCNGC 4 cut(s) 46, 244, 392, 811
Bme1390I CCNGG 4 cut(s) 151, 423, 496, 744
Bme18I GGWCC 2 cut(s) 428, 435
BmeT110I CYCGRG 1 cut(s) 813
BmgT120I GGNCC 2 cut(s) 428, 435
BmiI GGNNCC 1 cut(s) 625
BmrFI CCNGG 4 cut(s) 151, 423, 496, 744
BmsI GCATC 1 cut(s) 196
BpmI CTGGAG 2 cut(s) 329, 759
Bpu14I TTCGAA 1 cut(s) 1081
BpuMI CCSGG 2 cut(s) 151, 496
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 4 cut(s) 150, 237, 421, 431
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 352, 422
Bse1I ACTGG 3 cut(s) 658, 776, 1023
BseBI CCWGG 2 cut(s) 423, 744
BseDI CCNNGG 4 cut(s) 150, 237, 421, 431
BseLI CCNNNNNNNGG 3 cut(s) 180, 352, 422
BseMII CTCAG 5 cut(s) 324, 470, 884, 998, 1158
BseNI ACTGG 3 cut(s) 658, 776, 1023
BsePI GCGCGC 1 cut(s) 81
BseRI GAGGAG 3 cut(s) 274, 298, 323
BseXI GCAGC 1 cut(s) 254
BseYI CCCAGC 1 cut(s) 161
Bsh1236I CGCG 2 cut(s) 83, 85
Bsh1285I CGRYCG 1 cut(s) 702
BsiEI CGRYCG 1 cut(s) 702
BsiHKAI GWGCWC 1 cut(s) 869
BsiHKCI CYCGRG 1 cut(s) 813
BsiSI CCGG 3 cut(s) 120, 150, 495
BslI CCNNNNNNNGG 3 cut(s) 180, 352, 422
BsmAI GTCTC 1 cut(s) 950
BsmI GAATGC 2 cut(s) 62, 1077
BsoBI CYCGRG 1 cut(s) 813
Bsp119I TTCGAA 1 cut(s) 1081
Bsp1286I GDGCHC 2 cut(s) 358, 869
Bsp143I GATC 5 cut(s) 104, 168, 192, 656, 736
BspACI CCGC 5 cut(s) 44, 187, 391, 418, 809
BspCNI CTCAG 5 cut(s) 323, 469, 883, 999, 1159
BspFNI CGCG 2 cut(s) 83, 85
BspHI TCATGA 1 cut(s) 648
BspLI GGNNCC 1 cut(s) 625
BspPI GGATC 3 cut(s) 163, 187, 664
BspQI GCTCTTC 1 cut(s) 915
BspT104I TTCGAA 1 cut(s) 1081
BsrI ACTGG 3 cut(s) 658, 776, 1023
BssECI CCNNGG 4 cut(s) 150, 237, 421, 431
BssHII GCGCGC 1 cut(s) 81
BssMI GATC 5 cut(s) 104, 168, 192, 656, 736
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 1 cut(s) 431
Bst2UI CCWGG 2 cut(s) 423, 744
Bst4CI ACNGT 4 cut(s) 609, 691, 700, 1090
Bst6I CTCTTC 1 cut(s) 915
BstACI GRCGYC 1 cut(s) 117
BstBI TTCGAA 1 cut(s) 1081
BstC8I GCNNGC 2 cut(s) 83, 807
BstDEI CTNAG 7 cut(s) 310, 456, 628, 754, 870, 1007, 1167
BstFNI CGCG 2 cut(s) 83, 85
BstHHI GCGC 2 cut(s) 83, 85
BstKTI GATC 5 cut(s) 107, 171, 195, 659, 739
BstMAI GTCTC 1 cut(s) 950
BstMBI GATC 5 cut(s) 104, 168, 192, 656, 736
BstMCI CGRYCG 1 cut(s) 702
BstMWI GCNNNNNNNGC 3 cut(s) 82, 815, 1074
BstNI CCWGG 2 cut(s) 423, 744
BstNSI RCATGY 2 cut(s) 932, 1128
BstSCI CCNGG 4 cut(s) 149, 421, 494, 742
BstUI CGCG 2 cut(s) 83, 85
BstV1I GCAGC 1 cut(s) 254
BsuI GTATCC 1 cut(s) 835
BtsI GCAGTG 1 cut(s) 845
BtsIMutI CAGTG 1 cut(s) 845
Cac8I GCNNGC 2 cut(s) 83, 807
CciI TCATGA 1 cut(s) 648
CfoI GCGC 2 cut(s) 83, 85
Cfr13I GGNCC 2 cut(s) 428, 435
CseI GACGC 1 cut(s) 125
Csp6I GTAC 3 cut(s) 247, 480, 892
CviAII CATG 9 cut(s) 301, 554, 592, 644, 649, 713, 770, 929, 1125
CviQI GTAC 3 cut(s) 247, 480, 892
DdeI CTNAG 7 cut(s) 310, 456, 628, 754, 870, 1007, 1167
DpnI GATC 5 cut(s) 106, 170, 194, 658, 738
DpnII GATC 5 cut(s) 104, 168, 192, 656, 736
DraIII CACNNNGTG 1 cut(s) 444
Eam1104I CTCTTC 1 cut(s) 915
EarI CTCTTC 1 cut(s) 915
Eco130I CCWWGG 1 cut(s) 431
Eco24I GRGCYC 1 cut(s) 358
Eco47I GGWCC 2 cut(s) 428, 435
Eco88I CYCGRG 1 cut(s) 813
EcoRII CCWGG 2 cut(s) 421, 742
EcoT14I CCWWGG 1 cut(s) 431
EcoT38I GRGCYC 1 cut(s) 358
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 9 cut(s) 304, 557, 595, 647, 652, 716, 773, 932, 1128
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FatI CATG 9 cut(s) 300, 553, 591, 643, 648, 712, 769, 928, 1124
FauI CCCGC 2 cut(s) 180, 425
FauNDI CATATG 1 cut(s) 179
FblI GTMKAC 1 cut(s) 695
Fnu4HI GCNGC 4 cut(s) 45, 243, 391, 810
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 4 cut(s) 45, 243, 391, 810
FspBI CTAG 2 cut(s) 432, 953
GlaI GCGC 2 cut(s) 82, 84
GluI GCNGC 4 cut(s) 45, 243, 391, 810
GsaI CCCAGC 1 cut(s) 165
GsuI CTGGAG 2 cut(s) 329, 759
HapII CCGG 3 cut(s) 120, 150, 495
HgaI GACGC 1 cut(s) 125
HhaI GCGC 2 cut(s) 83, 85
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 9 cut(s) 304, 557, 595, 647, 652, 716, 773, 932, 1128
Hin6I GCGC 2 cut(s) 81, 83
HinP1I GCGC 2 cut(s) 81, 83
HincII GTYRAC 2 cut(s) 696, 1164
HindII GTYRAC 2 cut(s) 696, 1164
HindIII AAGCTT 2 cut(s) 725, 833
HinfI GANTC 4 cut(s) 35, 305, 640, 909
HpaII CCGG 3 cut(s) 120, 150, 495
HphI GGTGA 3 cut(s) 202, 313, 1153
Hpy166II GTNNAC 5 cut(s) 292, 438, 696, 1144, 1164
Hpy188I TCNGA 8 cut(s) 34, 136, 313, 522, 750, 798, 944, 1008
Hpy188III TCNNGA 5 cut(s) 256, 346, 649, 1051, 1109
Hpy8I GTNNAC 5 cut(s) 292, 438, 696, 1144, 1164
Hpy99I CGWCG 3 cut(s) 97, 119, 700
HpyCH4III ACNGT 4 cut(s) 609, 691, 700, 1090
HpyCH4V TGCA 4 cut(s) 76, 468, 850, 932
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 815, 1074
HpyF3I CTNAG 7 cut(s) 310, 456, 628, 754, 870, 1007, 1167
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 9 cut(s) 304, 557, 595, 647, 652, 716, 773, 932, 1128
HspAI GCGC 2 cut(s) 81, 83
Kzo9I GATC 5 cut(s) 104, 168, 192, 656, 736
LguI GCTCTTC 1 cut(s) 915
LmnI GCTCC 4 cut(s) 336, 541, 778, 1121
Lsp1109I GCAGC 1 cut(s) 254
LweI GCATC 1 cut(s) 196
MaeI CTAG 2 cut(s) 432, 953
MaeIII GTNAC 1 cut(s) 1039
MalI GATC 5 cut(s) 106, 170, 194, 658, 738
MboI GATC 5 cut(s) 104, 168, 192, 656, 736
MboII GAAGA 5 cut(s) 103, 731, 874, 932, 1202
MfeI CAATTG 2 cut(s) 545, 996
MhlI GDGCHC 2 cut(s) 358, 869
MlyI GAGTC 2 cut(s) 299, 918
MroXI GAANNNNTTC 3 cut(s) 639, 881, 1194
MseI TTAA 4 cut(s) 510, 975, 980, 990
MslI CAYNNNNRTG 1 cut(s) 596
MspI CCGG 3 cut(s) 120, 150, 495
MspR9I CCNGG 4 cut(s) 151, 423, 496, 744
MunI CAATTG 2 cut(s) 545, 996
Mva1269I GAATGC 2 cut(s) 62, 1077
MvaI CCWGG 2 cut(s) 423, 744
MvnI CGCG 2 cut(s) 83, 85
MwoI GCNNNNNNNGC 3 cut(s) 82, 815, 1074
NciI CCSGG 2 cut(s) 151, 496
NdeI CATATG 1 cut(s) 179
NdeII GATC 5 cut(s) 104, 168, 192, 656, 736
NlaIII CATG 9 cut(s) 304, 557, 595, 647, 652, 716, 773, 932, 1128
NlaIV GGNNCC 1 cut(s) 625
NmeAIII GCCGAG 1 cut(s) 218
NspI RCATGY 2 cut(s) 932, 1128
NspV TTCGAA 1 cut(s) 1081
PagI TCATGA 1 cut(s) 648
PauI GCGCGC 1 cut(s) 81
PciSI GCTCTTC 1 cut(s) 915
PctI GAATGC 2 cut(s) 62, 1077
PdmI GAANNNNTTC 3 cut(s) 639, 881, 1194
PfeI GAWTC 2 cut(s) 35, 640
PkrI GCNGC 4 cut(s) 46, 244, 392, 811
PleI GAGTC 2 cut(s) 299, 917
PpsI GAGTC 2 cut(s) 299, 917
PshBI ATTAAT 1 cut(s) 975
Psp6I CCWGG 2 cut(s) 421, 742
PspFI CCCAGC 1 cut(s) 161
PspGI CCWGG 2 cut(s) 421, 742
PspN4I GGNNCC 1 cut(s) 625
PspPI GGNCC 2 cut(s) 428, 435
PteI GCGCGC 1 cut(s) 81
RsaI GTAC 3 cut(s) 248, 481, 893
RsaNI GTAC 3 cut(s) 247, 480, 892
RseI CAYNNNNRTG 1 cut(s) 596
SalI GTCGAC 1 cut(s) 694
SapI GCTCTTC 1 cut(s) 915
SaqAI TTAA 4 cut(s) 510, 975, 980, 990
SatI GCNGC 4 cut(s) 45, 243, 391, 810
Sau3AI GATC 5 cut(s) 104, 168, 192, 656, 736
Sau96I GGNCC 2 cut(s) 428, 435
SchI GAGTC 2 cut(s) 299, 918
ScrFI CCNGG 4 cut(s) 151, 423, 496, 744
SduI GDGCHC 2 cut(s) 358, 869
SfaNI GCATC 1 cut(s) 196
SfuI TTCGAA 1 cut(s) 1081
SinI GGWCC 2 cut(s) 428, 435
SmiMI CAYNNNNRTG 1 cut(s) 596
SsiI CCGC 5 cut(s) 44, 187, 391, 418, 809
SspI AATATT 1 cut(s) 583
SspMI CTAG 2 cut(s) 432, 953
StyD4I CCNGG 4 cut(s) 149, 421, 494, 742
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 4 cut(s) 609, 691, 700, 1090
TaqI TCGA 5 cut(s) 103, 114, 216, 695, 1081
TatI WGTACW 2 cut(s) 479, 891
TauI GCSGC 3 cut(s) 47, 393, 812
TfiI GAWTC 2 cut(s) 35, 640
Tru1I TTAA 4 cut(s) 510, 975, 980, 990
Tru9I TTAA 4 cut(s) 510, 975, 980, 990
TscAI CASTG 1 cut(s) 852
TseI GCWGC 1 cut(s) 242
TspDTI ATGAA 9 cut(s) 542, 580, 632, 701, 719, 758, 874, 933, 1044
TspGWI ACGGA 2 cut(s) 28, 277
TspRI CASTG 1 cut(s) 852
VpaK11BI GGWCC 2 cut(s) 428, 435
VspI ATTAAT 1 cut(s) 975
XapI RAATTY 6 cut(s) 145, 222, 266, 786, 1013, 1056
XceI RCATGY 2 cut(s) 932, 1128
XmaJI CCTAGG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 695
XmnI GAANNNNTTC 3 cut(s) 639, 881, 1194
XspI CTAG 2 cut(s) 432, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.