Rh6BG055100
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0007961
Physical Location & Seq
Forward (+)
8923905 .. 8927058
3154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG055100.1

Sequence Viewer

Length: 801 bp
ATGCAAAGTGCTCGCAAAACGACGCCGTTCCGGTCAATTTTCGACGCCGGAGCTCTGAAATCAGAGCTGGAAAACTCCGGGATCAAGCCCAGCTTCATGCCCTACATATGGAAGCAGGTGATCCAAAACCCCTCCGCCCAACTCGACCAAATCCCCTCCTTGCCCTCCGCCGCATACCCTCTTCTAAACTCCAAATTCAAAACCCTAACCTCCTCCGTCCACTCCGCCACCGACTCCTCCGACCACCTCACCACCAAGCTCCTCATCAAGCTCCAGAACGGCGCTCTGGTGGAGGCCGTGATCATGAGGTACGATAGCAGCTTGGGGAAGCACAATGGCAAGTCCCACCACGGCGGCGCAAGGTCCACCTTGTGCGTGTCGTCTCAGGTTGGTTGTAAAATGGGTTGCACGTTCTGTGCCACTGGGAGTATGGGGTTTAAGAGCAATTTGACCTCTGGGGAGATTGTGGAGCAGTTGGTTCATGCTTCTCGCATTTCGCCTATACGCAATGTTGTTTTCATGGGAATGGGGGAACCGTTGAATAACTATAATGCCTTAGTGGAAGCGATTCGTGTCATGACTGGATTACCATTTCAGCTGTCGCCAAAGAGAATCACAGTGTCAACGGTTGGTATTATTCATGCTATCAACAAGTTCCATAATGATCTCCCTGGACTGAATTTAGCAGTTTCACTGCATGCACCTGTCCAAGAAATTCGTTGTCAGATAATGCCTGCAGCTCGGGCCTTTCCCTTAGTAAAACTTATGGATTCACTGCAACAATATCAAAAGAACAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

266

Amino Acids

29.01

Weight (kDa)

9.68

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 127 - 214 6.6e-09 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 106
Acc36I ACCTGC 1 cut(s) 106
AciI CCGC 5 cut(s) 135, 168, 171, 225, 354
AclWI GGATC 2 cut(s) 89, 115
AcsI RAATTY 3 cut(s) 194, 679, 714
AcyI GRCGYC 2 cut(s) 23, 45
AdeI CACNNNGTG 1 cut(s) 372
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 1 cut(s) 108
AgsI TTSAA 2 cut(s) 199, 541
AjnI CCWGG 1 cut(s) 670
AluBI AGCT 8 cut(s) 53, 67, 93, 259, 271, 321, 598, 740
AluI AGCT 8 cut(s) 53, 67, 93, 259, 271, 321, 598, 740
Alw21I GWGCWC 2 cut(s) 13, 55
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 2 cut(s) 89, 115
Ama87I CYCGRG 1 cut(s) 741
AoxI GGCC 2 cut(s) 294, 744
ApeKI GCWGC 2 cut(s) 318, 737
ApoI RAATTY 3 cut(s) 194, 679, 714
Asp700I GAANNNNTTC 1 cut(s) 567
AspLEI GCGC 2 cut(s) 284, 359
AspS9I GGNCC 2 cut(s) 363, 744
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 2 cut(s) 130, 241
AvaI CYCGRG 1 cut(s) 741
AvaII GGWCC 1 cut(s) 363
BanII GRGCYC 1 cut(s) 55
Bbv12I GWGCWC 2 cut(s) 13, 55
BbvI GCAGC 2 cut(s) 330, 749
BceAI ACGGC 4 cut(s) 10, 281, 295, 367
BcgI CGANNNNNNTGC 1 cut(s) 27
BciT130I CCWGG 1 cut(s) 672
BclI TGATCA 1 cut(s) 300
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 387
BfmI CTRYAG 1 cut(s) 735
BfoI RGCGCY 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 106
BisI GCNGC 4 cut(s) 171, 319, 355, 738
BlsI GCNGC 4 cut(s) 172, 320, 356, 739
Bme1390I CCNGG 2 cut(s) 79, 672
Bme18I GGWCC 1 cut(s) 363
BmeT110I CYCGRG 1 cut(s) 741
BmgT120I GGNCC 2 cut(s) 363, 744
BmiI GGNNCC 1 cut(s) 534
BmrFI CCNGG 2 cut(s) 79, 672
BmrI ACTGGG 1 cut(s) 432
BmuI ACTGGG 1 cut(s) 432
BpmI CTGGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 79
BsaHI GRCGYC 2 cut(s) 23, 45
BsaJI CCNNGG 2 cut(s) 349, 670
BsaWI WCCGGW 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse1I ACTGG 2 cut(s) 427, 586
Bse3DI GCAATG 1 cut(s) 514
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 2 cut(s) 349, 670
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 1 cut(s) 398
BseNI ACTGG 2 cut(s) 427, 586
BseRI GAGGAG 3 cut(s) 202, 226, 251
BseXI GCAGC 2 cut(s) 330, 749
BseYI CCCAGC 1 cut(s) 89
BshFI GGCC 2 cut(s) 296, 746
BsiHKAI GWGCWC 2 cut(s) 13, 55
BsiHKCI CYCGRG 1 cut(s) 741
BsiSI CCGG 3 cut(s) 31, 48, 78
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 108
BsmAI GTCTC 1 cut(s) 387
BsmBI CGTCTC 1 cut(s) 387
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 2 cut(s) 296, 746
BsoBI CYCGRG 1 cut(s) 741
Bsp1286I GDGCHC 2 cut(s) 13, 55
Bsp143I GATC 4 cut(s) 81, 120, 300, 664
BspACI CCGC 5 cut(s) 135, 168, 171, 225, 354
BspANI GGCC 2 cut(s) 296, 746
BspCNI CTCAG 1 cut(s) 397
BspHI TCATGA 2 cut(s) 303, 576
BspLI GGNNCC 1 cut(s) 534
BspMAI CTGCAG 1 cut(s) 739
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 2 cut(s) 89, 115
BsrDI GCAATG 1 cut(s) 514
BsrI ACTGG 2 cut(s) 427, 586
BssECI CCNNGG 2 cut(s) 349, 670
BssMI GATC 4 cut(s) 81, 120, 300, 664
BssNI GRCGYC 2 cut(s) 23, 45
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 3 cut(s) 537, 619, 628
Bst6I CTCTTC 1 cut(s) 186
BstACI GRCGYC 2 cut(s) 23, 45
BstC8I GCNNGC 3 cut(s) 13, 699, 735
BstDEI CTNAG 3 cut(s) 384, 556, 754
BstDSI CCRYGG 1 cut(s) 349
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 2 cut(s) 284, 359
BstKTI GATC 4 cut(s) 84, 123, 303, 667
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 4 cut(s) 81, 120, 300, 664
BstMWI GCNNNNNNNGC 1 cut(s) 743
BstNI CCWGG 1 cut(s) 672
BstNSI RCATGY 1 cut(s) 701
BstSCI CCNGG 2 cut(s) 77, 670
BstSFI CTRYAG 1 cut(s) 735
BstV1I GCAGC 2 cut(s) 330, 749
BsuRI GGCC 2 cut(s) 296, 746
BtgI CCRYGG 1 cut(s) 349
BtsI GCAGTG 2 cut(s) 692, 773
BtsIMutI CAGTG 4 cut(s) 420, 624, 692, 773
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 3 cut(s) 13, 699, 735
CciI TCATGA 2 cut(s) 303, 576
CfoI GCGC 2 cut(s) 284, 359
Cfr13I GGNCC 2 cut(s) 363, 744
CseI GACGC 2 cut(s) 31, 53
Csp6I GTAC 1 cut(s) 310
CviAII CATG 7 cut(s) 97, 304, 482, 520, 577, 641, 698
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 3 cut(s) 384, 556, 754
DpnI GATC 4 cut(s) 83, 122, 302, 666
DpnII GATC 4 cut(s) 81, 120, 300, 664
DraIII CACNNNGTG 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
EciI GGCGGA 3 cut(s) 124, 157, 214
Ecl136II GAGCTC 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 363
Eco53kI GAGCTC 1 cut(s) 53
Eco88I CYCGRG 1 cut(s) 741
EcoICRI GAGCTC 1 cut(s) 53
EcoRII CCWGG 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 55
Esp3I CGTCTC 1 cut(s) 387
FaeI CATG 7 cut(s) 100, 307, 485, 523, 580, 644, 701
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FaqI GGGAC 1 cut(s) 328
FatI CATG 7 cut(s) 96, 303, 481, 519, 576, 640, 697
FauNDI CATATG 1 cut(s) 107
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 4 cut(s) 171, 319, 355, 738
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 4 cut(s) 171, 319, 355, 738
GlaI GCGC 2 cut(s) 283, 358
GluI GCNGC 4 cut(s) 171, 319, 355, 738
GsaI CCCAGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 2 cut(s) 296, 746
HapII CCGG 3 cut(s) 31, 48, 78
HgaI GACGC 2 cut(s) 31, 53
HhaI GCGC 2 cut(s) 284, 359
Hin1I GRCGYC 2 cut(s) 23, 45
Hin1II CATG 7 cut(s) 100, 307, 485, 523, 580, 644, 701
Hin6I GCGC 2 cut(s) 282, 357
HinP1I GCGC 2 cut(s) 282, 357
HincII GTYRAC 1 cut(s) 624
HindII GTYRAC 1 cut(s) 624
HinfI GANTC 4 cut(s) 233, 568, 612, 770
HpaII CCGG 3 cut(s) 31, 48, 78
HphI GGTGA 2 cut(s) 130, 241
Hpy166II GTNNAC 3 cut(s) 220, 366, 624
Hpy188I TCNGA 4 cut(s) 57, 64, 241, 726
Hpy188III TCNNGA 3 cut(s) 274, 304, 577
Hpy8I GTNNAC 3 cut(s) 220, 366, 624
Hpy99I CGWCG 2 cut(s) 25, 47
HpyCH4III ACNGT 3 cut(s) 537, 619, 628
HpyCH4IV ACGT 1 cut(s) 410
HpyCH4V TGCA 6 cut(s) 4, 408, 697, 701, 737, 778
HpyF10VI GCNNNNNNNGC 1 cut(s) 743
HpyF3I CTNAG 3 cut(s) 384, 556, 754
HpySE526I ACGT 1 cut(s) 410
Hsp92I GRCGYC 2 cut(s) 23, 45
Hsp92II CATG 7 cut(s) 100, 307, 485, 523, 580, 644, 701
HspAI GCGC 2 cut(s) 282, 357
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 4 cut(s) 81, 120, 300, 664
LmnI GCTCC 4 cut(s) 50, 264, 276, 469
Lsp1109I GCAGC 2 cut(s) 330, 749
MaeII ACGT 1 cut(s) 410
MalI GATC 4 cut(s) 83, 122, 302, 666
MboI GATC 4 cut(s) 81, 120, 300, 664
MboII GAAGA 1 cut(s) 173
MhlI GDGCHC 2 cut(s) 13, 55
MluCI AATT 5 cut(s) 36, 194, 445, 679, 714
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 264
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 1 cut(s) 438
MslI CAYNNNNRTG 1 cut(s) 524
MspA1I CMGCKG 1 cut(s) 598
MspI CCGG 3 cut(s) 31, 48, 78
MspR9I CCNGG 2 cut(s) 79, 672
MvaI CCWGG 1 cut(s) 672
MwoI GCNNNNNNNGC 1 cut(s) 743
NciI CCSGG 1 cut(s) 79
NdeI CATATG 1 cut(s) 107
NdeII GATC 4 cut(s) 81, 120, 300, 664
NlaIII CATG 7 cut(s) 100, 307, 485, 523, 580, 644, 701
NlaIV GGNNCC 1 cut(s) 534
NspI RCATGY 1 cut(s) 701
PaeI GCATGC 1 cut(s) 701
PagI TCATGA 2 cut(s) 303, 576
PaqCI CACCTGC 1 cut(s) 106
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 3 cut(s) 568, 612, 770
PfoI TCCNGGA 1 cut(s) 77
PkrI GCNGC 4 cut(s) 172, 320, 356, 739
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
Psp124BI GAGCTC 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 670
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 1 cut(s) 534
PspPI GGNCC 2 cut(s) 363, 744
PstI CTGCAG 1 cut(s) 739
PvuII CAGCTG 1 cut(s) 598
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 524
SacI GAGCTC 1 cut(s) 55
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 4 cut(s) 171, 319, 355, 738
Sau3AI GATC 4 cut(s) 81, 120, 300, 664
Sau96I GGNCC 2 cut(s) 363, 744
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 2 cut(s) 79, 672
SduI GDGCHC 2 cut(s) 13, 55
SfcI CTRYAG 1 cut(s) 735
SinI GGWCC 1 cut(s) 363
SmiMI CAYNNNNRTG 1 cut(s) 524
SphI GCATGC 1 cut(s) 701
Sse9I AATT 5 cut(s) 36, 194, 445, 679, 714
SsiI CCGC 5 cut(s) 135, 168, 171, 225, 354
SstI GAGCTC 1 cut(s) 55
StyD4I CCNGG 2 cut(s) 77, 670
TaaI ACNGT 3 cut(s) 537, 619, 628
TaiI ACGT 1 cut(s) 413
TaqI TCGA 2 cut(s) 42, 144
TasI AATT 5 cut(s) 36, 194, 445, 679, 714
TauI GCSGC 2 cut(s) 173, 357
TfiI GAWTC 3 cut(s) 568, 612, 770
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TscAI CASTG 4 cut(s) 427, 624, 699, 780
TseI GCWGC 2 cut(s) 318, 737
TspDTI ATGAA 4 cut(s) 85, 470, 508, 629
TspGWI ACGGA 1 cut(s) 205
TspRI CASTG 4 cut(s) 427, 624, 699, 780
VpaK11BI GGWCC 1 cut(s) 363
XapI RAATTY 3 cut(s) 194, 679, 714
XceI RCATGY 1 cut(s) 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.