RchiOBHm_Chr6g0253741
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007961
Physical Location & Seq
Forward (+)
8860697 .. 8863593
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22755

Sequence Viewer

Length: 1362 bp
ATGAAATTCAATAGGAAAAACCTTTACTTTCCAAGTAGATGTGGTTTTTTTTTTTTTTTTTTCTATAGAAACAAGATTTTCTTTTTTGTGCCAAAACAAAAAATTTACTTCGACTCGGTTACGACGTCGTTTCCTTTTAGAAGTGGTCTTCCCTCTTTGCATAGGATTTCACAGCGAAGTCATAACTCATCCGCCTCCGTCACCGCAACCCCCAAAACGCCCAAAATCATGCAAAGTCCTCGCAAAACGACGCCGTTCCGGTCAATTTTCGACGCCGGAGCTCTGAAATCAGAGCTGGAAAACTCCGGAATCAAGCCCAGCTTCATGCCCTACATATGGAAGCAGGTGATCCAAAACCCCTCCGCCCAACTCGACCAAATCCCCTCCTTGCCCTCCGCCGCATACCCTCTTCTCAACTCCAAATTCAAAACCCTAACCTCCTCCGTCCACTCCGCCACCGACTCCTCCGACCACCTCACCACCAAGCTCCTCATCAAGCTCCAGAATGGCGCTCTGGTGGAGGCCGTGATCATGAGGTACGATAGCAGCTTGGGGAAGCACAATGGCAAGTCCCACCACGGCGGCGCAAGGTCCACCTTGTGCGTGTCGTCTCAGGTTGGTTGTAAAATGGGTTGCACGTTCTGTGCCACTGGGAGTATGGGGTTTAAGAGCAATTTGACCTCTGGGGAGATTGTGGAGCAGTTGGTTCATGCTTCTCGCATTTCGCCTATACGCAATGTTGTTTTCATGGGAATGGGGGAACCGTTGAACAACTATAATGCCTTAGTGGAAGCAATTCATGTCATGACTGGATTACCATTTCAGCTGTCGCCAAAGAGAATCACAGTGTCAACGGTTGGTATTATTCATGCTATCAACAAGTTCCATAATGATCTCCCTGGACTGAATTTAGCAGTTTCACTGCATGCACCTGTCCAAGAAATTCGTTGTCAGATAATGCCTGCAGCTCGGGCCTTTCCCTTAGTAAAACTTATGGATTCACTGCAACAATATCAAAAGAACAGTCAGCAGAAAATTTTCATTGAATACATAATGCTTGACGGAGTGAACGATGAAGAAGAACATGCGCACCAGCTTGGGAAATTGCTAGATACATTTCAAGTGGTAATTAATTTAATACCTTTTAACCCAATTGGTAGTCTGAGCAAATTCAAAACCACTGCCGAAGAAAAAGTATCTGTTTTTCAGAAGATTCTAAGAGGTACGTATGGCATCCGAACAACCGTTCGCAAGGAAATGGGTCAGGACATAAGTGGAGCGTGTGGTCAGTTGGTGGTGAACCTACCCAATAAGAGGTCAAGTGACCCTCTAACTGATATAGAAGATCTTCAGGTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

453

Amino Acids

50.47

Weight (kDa)

9.67

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 203 - 363 5.1e-11 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 334
AatII GACGTC 1 cut(s) 128
Acc16I TGCGCA 1 cut(s) 1089
Acc36I ACCTGC 1 cut(s) 334
AccIII TCCGGA 1 cut(s) 305
AciI CCGC 7 cut(s) 192, 204, 363, 396, 399, 453, 582
AclWI GGATC 1 cut(s) 343
AcsI RAATTY 7 cut(s) 5, 102, 422, 907, 942, 1035, 1169
AcuI CTGAAG 1 cut(s) 1334
AcyI GRCGYC 3 cut(s) 125, 251, 273
AdeI CACNNNGTG 1 cut(s) 600
AfaI GTAC 2 cut(s) 539, 1225
AfiI CCNNNNNNNGG 2 cut(s) 336, 1314
AgsI TTSAA 6 cut(s) 10, 427, 769, 1046, 1121, 1174
AjnI CCWGG 1 cut(s) 898
AluBI AGCT 9 cut(s) 281, 295, 321, 487, 499, 549, 826, 968, 1096
AluI AGCT 9 cut(s) 281, 295, 321, 487, 499, 549, 826, 968, 1096
Alw21I GWGCWC 1 cut(s) 283
Alw26I GTCTC 1 cut(s) 615
AlwI GGATC 1 cut(s) 343
Ama87I CYCGRG 1 cut(s) 969
Aor13HI TCCGGA 1 cut(s) 305
AoxI GGCC 2 cut(s) 522, 972
ApeKI GCWGC 2 cut(s) 546, 965
ApoI RAATTY 7 cut(s) 5, 102, 422, 907, 942, 1035, 1169
ArsI GACNNNNNNTTYG 2 cut(s) 1009, 1041
AseI ATTAAT 1 cut(s) 1131
Asp700I GAANNNNTTC 3 cut(s) 795, 1037, 1347
AspLEI GCGC 3 cut(s) 512, 587, 1090
AspS9I GGNCC 2 cut(s) 591, 972
AsuHPI GGTGA 4 cut(s) 193, 358, 469, 1309
AvaI CYCGRG 1 cut(s) 969
AvaII GGWCC 1 cut(s) 591
BanII GRGCYC 1 cut(s) 283
BbsI GAAGAC 1 cut(s) 140
Bbv12I GWGCWC 1 cut(s) 283
BbvI GCAGC 2 cut(s) 558, 977
BceAI ACGGC 3 cut(s) 238, 509, 595
BcgI CGANNNNNNTGC 2 cut(s) 221, 255
BciT130I CCWGG 1 cut(s) 900
BclI TGATCA 1 cut(s) 528
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 1 cut(s) 1109
BfmI CTRYAG 2 cut(s) 64, 963
BfoI RGCGCY 1 cut(s) 513
BfuAI ACCTGC 1 cut(s) 334
BglII AGATCT 1 cut(s) 1345
BisI GCNGC 4 cut(s) 399, 547, 583, 966
BlsI GCNGC 4 cut(s) 400, 548, 584, 967
Bme1390I CCNGG 1 cut(s) 900
Bme18I GGWCC 1 cut(s) 591
BmeT110I CYCGRG 1 cut(s) 969
BmgT120I GGNCC 2 cut(s) 591, 972
BmiI GGNNCC 1 cut(s) 762
BmrFI CCNGG 1 cut(s) 900
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 1 cut(s) 1242
BmuI ACTGGG 1 cut(s) 660
BpiI GAAGAC 1 cut(s) 140
BpmI CTGGAG 1 cut(s) 485
BsaAI YACGTR 1 cut(s) 1227
BsaHI GRCGYC 3 cut(s) 125, 251, 273
BsaJI CCNNGG 2 cut(s) 577, 898
BsaWI WCCGGW 2 cut(s) 258, 305
Bsc4I CCNNNNNNNGG 2 cut(s) 336, 1314
Bse1I ACTGG 2 cut(s) 655, 814
Bse3DI GCAATG 1 cut(s) 742
BseAI TCCGGA 1 cut(s) 305
BseBI CCWGG 1 cut(s) 900
BseDI CCNNGG 2 cut(s) 577, 898
BseGI GGATG 2 cut(s) 188, 1233
BseLI CCNNNNNNNGG 2 cut(s) 336, 1314
BseMI GCAATG 1 cut(s) 742
BseMII CTCAG 2 cut(s) 626, 1154
BseNI ACTGG 2 cut(s) 655, 814
BseRI GAGGAG 3 cut(s) 430, 454, 479
BseXI GCAGC 2 cut(s) 558, 977
BseYI CCCAGC 1 cut(s) 317
BshFI GGCC 2 cut(s) 524, 974
BsiHKAI GWGCWC 1 cut(s) 283
BsiHKCI CYCGRG 1 cut(s) 969
BsiSI CCGG 3 cut(s) 259, 276, 306
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 2 cut(s) 336, 1314
BsmAI GTCTC 1 cut(s) 615
BsmBI CGTCTC 1 cut(s) 615
BsmFI GGGAC 1 cut(s) 556
BsnI GGCC 2 cut(s) 524, 974
BsoBI CYCGRG 1 cut(s) 969
Bsp1286I GDGCHC 1 cut(s) 283
Bsp13I TCCGGA 1 cut(s) 305
Bsp143I GATC 4 cut(s) 348, 528, 892, 1345
BspACI CCGC 7 cut(s) 192, 204, 363, 396, 399, 453, 582
BspANI GGCC 2 cut(s) 524, 974
BspCNI CTCAG 2 cut(s) 625, 1155
BspEI TCCGGA 1 cut(s) 305
BspHI TCATGA 2 cut(s) 531, 804
BspLI GGNNCC 1 cut(s) 762
BspMAI CTGCAG 1 cut(s) 967
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 742
BsrI ACTGG 2 cut(s) 655, 814
BssECI CCNNGG 2 cut(s) 577, 898
BssMI GATC 4 cut(s) 348, 528, 892, 1345
BssNI GRCGYC 3 cut(s) 125, 251, 273
Bst2UI CCWGG 1 cut(s) 900
Bst4CI ACNGT 5 cut(s) 765, 847, 856, 1025, 1246
Bst6I CTCTTC 1 cut(s) 414
BstACI GRCGYC 3 cut(s) 125, 251, 273
BstBAI YACGTR 1 cut(s) 1227
BstC8I GCNNGC 2 cut(s) 927, 963
BstDEI CTNAG 5 cut(s) 612, 784, 982, 1163, 1217
BstDSI CCRYGG 1 cut(s) 577
BstF5I GGATG 2 cut(s) 188, 1233
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 3 cut(s) 512, 587, 1090
BstKTI GATC 4 cut(s) 351, 531, 895, 1348
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 4 cut(s) 348, 528, 892, 1345
BstMWI GCNNNNNNNGC 1 cut(s) 971
BstNI CCWGG 1 cut(s) 900
BstNSI RCATGY 2 cut(s) 929, 1088
BstSCI CCNGG 1 cut(s) 898
BstSFI CTRYAG 2 cut(s) 64, 963
BstSNI TACGTA 1 cut(s) 1227
BstV1I GCAGC 2 cut(s) 558, 977
BstV2I GAAGAC 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 1345
BstYI RGATCY 1 cut(s) 1345
BsuRI GGCC 2 cut(s) 524, 974
BtgI CCRYGG 1 cut(s) 577
BtsCI GGATG 2 cut(s) 188, 1233
BtsI GCAGTG 3 cut(s) 920, 1001, 1179
BtsIMutI CAGTG 5 cut(s) 648, 852, 920, 1001, 1179
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 2 cut(s) 927, 963
CciI TCATGA 2 cut(s) 531, 804
CfoI GCGC 3 cut(s) 512, 587, 1090
Cfr13I GGNCC 2 cut(s) 591, 972
CseI GACGC 2 cut(s) 259, 281
Csp6I GTAC 2 cut(s) 538, 1224
CviQI GTAC 2 cut(s) 538, 1224
DdeI CTNAG 5 cut(s) 612, 784, 982, 1163, 1217
DpnI GATC 4 cut(s) 350, 530, 894, 1347
DpnII GATC 4 cut(s) 348, 528, 892, 1345
DraIII CACNNNGTG 1 cut(s) 600
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
EciI GGCGGA 4 cut(s) 181, 352, 385, 442
Ecl136II GAGCTC 1 cut(s) 281
Eco105I TACGTA 1 cut(s) 1227
Eco24I GRGCYC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 591
Eco53kI GAGCTC 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 1334
Eco88I CYCGRG 1 cut(s) 969
EcoICRI GAGCTC 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 283
Esp3I CGTCTC 1 cut(s) 615
FalI AAGNNNNNCTT 4 cut(s) 65, 97, 305, 337
FaqI GGGAC 1 cut(s) 556
FauNDI CATATG 1 cut(s) 335
FbaI TGATCA 1 cut(s) 528
Fnu4HI GCNGC 4 cut(s) 399, 547, 583, 966
FokI GGATG 2 cut(s) 175, 1220
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 4 cut(s) 399, 547, 583, 966
FspAI RTGCGCAY 1 cut(s) 1089
FspBI CTAG 1 cut(s) 1109
FspI TGCGCA 1 cut(s) 1089
GlaI GCGC 3 cut(s) 511, 586, 1089
GluI GCNGC 4 cut(s) 399, 547, 583, 966
GsaI CCCAGC 1 cut(s) 321
GsuI CTGGAG 1 cut(s) 485
HaeII RGCGCY 1 cut(s) 513
HaeIII GGCC 2 cut(s) 524, 974
HapII CCGG 3 cut(s) 259, 276, 306
HgaI GACGC 2 cut(s) 259, 281
HhaI GCGC 3 cut(s) 512, 587, 1090
Hin1I GRCGYC 3 cut(s) 125, 251, 273
Hin6I GCGC 3 cut(s) 510, 585, 1088
HinP1I GCGC 3 cut(s) 510, 585, 1088
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HinfI GANTC 6 cut(s) 113, 309, 461, 840, 998, 1213
HpaII CCGG 3 cut(s) 259, 276, 306
HphI GGTGA 4 cut(s) 193, 358, 469, 1309
Hpy166II GTNNAC 5 cut(s) 448, 594, 852, 1069, 1300
Hpy188I TCNGA 8 cut(s) 285, 292, 469, 954, 1164, 1209, 1238, 1358
Hpy188III TCNNGA 5 cut(s) 306, 502, 532, 805, 1265
Hpy8I GTNNAC 5 cut(s) 448, 594, 852, 1069, 1300
Hpy99I CGWCG 4 cut(s) 127, 130, 253, 275
HpyCH4III ACNGT 5 cut(s) 765, 847, 856, 1025, 1246
HpyCH4IV ACGT 3 cut(s) 125, 638, 1226
HpyCH4V TGCA 7 cut(s) 160, 232, 636, 925, 929, 965, 1006
HpyF10VI GCNNNNNNNGC 1 cut(s) 971
HpyF3I CTNAG 5 cut(s) 612, 784, 982, 1163, 1217
HpySE526I ACGT 3 cut(s) 125, 638, 1226
Hsp92I GRCGYC 3 cut(s) 125, 251, 273
HspAI GCGC 3 cut(s) 510, 585, 1088
Kpn2I TCCGGA 1 cut(s) 305
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 4 cut(s) 348, 528, 892, 1345
LmnI GCTCC 5 cut(s) 278, 492, 504, 697, 1277
Lsp1109I GCAGC 2 cut(s) 558, 977
LweI GCATC 1 cut(s) 1242
MaeI CTAG 1 cut(s) 1109
MaeII ACGT 3 cut(s) 125, 638, 1226
MaeIII GTNAC 3 cut(s) 118, 199, 1322
MalI GATC 4 cut(s) 350, 530, 894, 1347
MboI GATC 4 cut(s) 348, 528, 892, 1345
MboII GAAGA 8 cut(s) 140, 401, 1088, 1091, 1199, 1222, 1340, 1355
MfeI CAATTG 1 cut(s) 1152
MflI RGATCY 1 cut(s) 1345
MhlI GDGCHC 1 cut(s) 283
MlyI GAGTC 2 cut(s) 107, 455
MmeI TCCRAC 1 cut(s) 492
MroI TCCGGA 1 cut(s) 305
MroXI GAANNNNTTC 3 cut(s) 795, 1037, 1347
MseI TTAA 4 cut(s) 666, 1131, 1136, 1146
MslI CAYNNNNRTG 1 cut(s) 752
MspA1I CMGCKG 1 cut(s) 826
MspI CCGG 3 cut(s) 259, 276, 306
MspR9I CCNGG 1 cut(s) 900
MunI CAATTG 1 cut(s) 1152
MvaI CCWGG 1 cut(s) 900
MwoI GCNNNNNNNGC 1 cut(s) 971
NdeI CATATG 1 cut(s) 335
NdeII GATC 4 cut(s) 348, 528, 892, 1345
NlaIV GGNNCC 1 cut(s) 762
NmuCI GTSAC 2 cut(s) 199, 1322
NsbI TGCGCA 1 cut(s) 1089
NspI RCATGY 2 cut(s) 929, 1088
PaeI GCATGC 1 cut(s) 929
PagI TCATGA 2 cut(s) 531, 804
PaqCI CACCTGC 1 cut(s) 334
PcsI WCGNNNNNNNCGW 2 cut(s) 122, 1068
PdmI GAANNNNTTC 3 cut(s) 795, 1037, 1347
PfeI GAWTC 4 cut(s) 309, 840, 998, 1213
PkrI GCNGC 4 cut(s) 400, 548, 584, 967
PleI GAGTC 2 cut(s) 107, 455
PpsI GAGTC 2 cut(s) 107, 455
Ppu21I YACGTR 1 cut(s) 1227
PshBI ATTAAT 1 cut(s) 1131
Psp124BI GAGCTC 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 898
PspFI CCCAGC 1 cut(s) 317
PspGI CCWGG 1 cut(s) 898
PspN4I GGNNCC 1 cut(s) 762
PspPI GGNCC 2 cut(s) 591, 972
PstI CTGCAG 1 cut(s) 967
PsuI RGATCY 1 cut(s) 1345
PvuII CAGCTG 1 cut(s) 826
RsaI GTAC 2 cut(s) 539, 1225
RsaNI GTAC 2 cut(s) 538, 1224
RseI CAYNNNNRTG 1 cut(s) 752
SacI GAGCTC 1 cut(s) 283
SaqAI TTAA 4 cut(s) 666, 1131, 1136, 1146
SatI GCNGC 4 cut(s) 399, 547, 583, 966
Sau3AI GATC 4 cut(s) 348, 528, 892, 1345
Sau96I GGNCC 2 cut(s) 591, 972
SchI GAGTC 2 cut(s) 107, 455
ScrFI CCNGG 1 cut(s) 900
SduI GDGCHC 1 cut(s) 283
SfaNI GCATC 1 cut(s) 1242
SfcI CTRYAG 2 cut(s) 64, 963
SinI GGWCC 1 cut(s) 591
SmiMI CAYNNNNRTG 1 cut(s) 752
SnaBI TACGTA 1 cut(s) 1227
SphI GCATGC 1 cut(s) 929
SsiI CCGC 7 cut(s) 192, 204, 363, 396, 399, 453, 582
SspMI CTAG 1 cut(s) 1109
SstI GAGCTC 1 cut(s) 283
StyD4I CCNGG 1 cut(s) 898
TaaI ACNGT 5 cut(s) 765, 847, 856, 1025, 1246
TaiI ACGT 3 cut(s) 128, 641, 1229
TaqI TCGA 3 cut(s) 111, 270, 372
TauI GCSGC 2 cut(s) 401, 585
TfiI GAWTC 4 cut(s) 309, 840, 998, 1213
Tru1I TTAA 4 cut(s) 666, 1131, 1136, 1146
Tru9I TTAA 4 cut(s) 666, 1131, 1136, 1146
TscAI CASTG 5 cut(s) 655, 852, 927, 1008, 1186
TseFI GTSAC 2 cut(s) 199, 1322
TseI GCWGC 2 cut(s) 546, 965
Tsp45I GTSAC 2 cut(s) 199, 1322
TspDTI ATGAA 8 cut(s) 17, 313, 698, 736, 788, 857, 1030, 1089
TspGWI ACGGA 3 cut(s) 187, 433, 1077
TspRI CASTG 5 cut(s) 655, 852, 927, 1008, 1186
VpaK11BI GGWCC 1 cut(s) 591
VspI ATTAAT 1 cut(s) 1131
XapI RAATTY 7 cut(s) 5, 102, 422, 907, 942, 1035, 1169
XceI RCATGY 2 cut(s) 929, 1088
XcmI CCANNNNNNNNNTGG 1 cut(s) 655
XmnI GAANNNNTTC 3 cut(s) 795, 1037, 1347
XspI CTAG 1 cut(s) 1109
ZraI GACGTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.