Prupe.8G144200_v2.0.a1
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0007961
Physical Location & Seq
Forward (+)
16076681 .. 16079536
2856 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G144200.1

Sequence Viewer

Length: 1134 bp
ATGCAAAGTGGAAAAAATACGATGTCGTTTCGATCAATCTTCGACGCGGGAGAGCTGAAATCAGAACTGGAAAAGTCCGGGGTCAAACCCAGCTTCATGCCTTACATATGGAAGCAGGTGATCCAAAACCCCGACGCCCAATTGGACCAAATTCCGTCTTTACCCTCCGCCGCGTACCCTCTTCTAACCTCAAAGTTCAAAACCCTAACCTCCTGCGTCCACTCGGCCATGGATTCCTCAGACCAACTTACCACCAAGCTCCTTATAAAGCTACAGAATGGGGCTCTTGTGGAGGCTGTGATTATGAGGTACGATACCCGCTTGGGAAAGTACAATGGGAAGCCCCGCCCTGGTGGAATGAGGTCCACCTTGTGCGTATCATCTCAGGTTGGTTGCAAAATGGGTTGCACATTCTGTGCCACTGGGAGTATGGGGTTTAAGAGCAATCTGACCTCGGGGGAGATTGTGGAGCAACTGGTTCATGCTTCTCGTATTTCGCCTATACGCAATGTTGTTTTCATGGGAATGGGGGAACCATTGAATAACTATGGTGCCTTGGTGGAAGCGATTCATGTCATGACTGGACCACCATTTCATTTGTCGCCAAAGAAAATTACTGTGTCCACGGTTGGCATTATTCATGCTATCAAAAAGCTTCATATTGACCTTCCAGGACTGAATTTAGCAGTTTCACTACATGCACCTGTACAAGAAATTCGTTGTCAGATAATGCCTGCAGCTCGAGCTTTTCCTTTAGTAAAGCTTATGGATACACTACAAGAATATCAAAAGAACACTCAGCAAAAAATCTTCATTGAGTACATAATGCTTGATGGAGTGAATGATGAAGAGCAAAATGCACACCAGCTCGGGAAATTGCTAGAGACATTTCAAGTGGTGGTTAATCTAATACCCTTTAATCCAATTGGTGATCTGAGTAAGTTTAGCACCAGTAACGAAGAGAAAGTATCAAGTTTCCAGAAAATCTTACGAGGTACTTATGGCATTCGAACAACAGTTCGTAAGGAAATGGGTCAGGACATAAGTGGAGCATGTGGGCAGTTGGTTGTGAACAAGAGTTCAATTGAGAACACAGTTCTCTTAACTGATATAGAAGATCTTCAGGTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

378

Amino Acids

41.52

Weight (kDa)

8.83

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 266
AarI CACCTGC 1 cut(s) 106
Acc36I ACCTGC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 551
AccII CGCG 2 cut(s) 47, 173
AciI CCGC 5 cut(s) 47, 168, 171, 319, 346
AclWI GGATC 1 cut(s) 115
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 3 cut(s) 150, 679, 714
AcuI CTGAAG 1 cut(s) 1106
AcyI GRCGYC 1 cut(s) 135
AdeI CACNNNGTG 1 cut(s) 372
AfaI GTAC 6 cut(s) 176, 311, 332, 708, 821, 997
AfiI CCNNNNNNNGG 1 cut(s) 350
AgsI TTSAA 4 cut(s) 199, 541, 893, 1083
AjnI CCWGG 2 cut(s) 349, 670
AluBI AGCT 9 cut(s) 55, 93, 259, 271, 655, 740, 746, 763, 868
AluI AGCT 9 cut(s) 55, 93, 259, 271, 655, 740, 746, 763, 868
Alw26I GTCTC 1 cut(s) 878
AlwI GGATC 1 cut(s) 115
Ama87I CYCGRG 3 cut(s) 454, 741, 869
AoxI GGCC 1 cut(s) 225
ApeKI GCWGC 1 cut(s) 737
ApoI RAATTY 3 cut(s) 150, 679, 714
Asp700I GAANNNNTTC 2 cut(s) 567, 1119
AspS9I GGNCC 3 cut(s) 145, 363, 584
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 2 cut(s) 130, 941
AsuII TTCGAA 1 cut(s) 1009
AvaI CYCGRG 3 cut(s) 454, 741, 869
AvaII GGWCC 3 cut(s) 145, 363, 584
BanI GGYRCC 1 cut(s) 551
BanII GRGCYC 1 cut(s) 286
BbvI GCAGC 1 cut(s) 749
BccI CCATC 1 cut(s) 827
BciT130I CCWGG 2 cut(s) 351, 672
BciVI GTATCC 1 cut(s) 763
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 878
BfaI CTAG 1 cut(s) 881
BfmI CTRYAG 2 cut(s) 272, 735
BfuAI ACCTGC 1 cut(s) 106
BfuI GTATCC 1 cut(s) 763
BglII AGATCT 1 cut(s) 1117
BisI GCNGC 2 cut(s) 171, 738
BlsI GCNGC 2 cut(s) 172, 739
Bme1390I CCNGG 3 cut(s) 79, 351, 672
Bme18I GGWCC 3 cut(s) 145, 363, 584
BmeT110I CYCGRG 3 cut(s) 454, 741, 869
BmgT120I GGNCC 3 cut(s) 145, 363, 584
BmiI GGNNCC 2 cut(s) 534, 553
BmrFI CCNGG 3 cut(s) 79, 351, 672
BmrI ACTGGG 1 cut(s) 432
BmuI ACTGGG 1 cut(s) 432
Bpu14I TTCGAA 1 cut(s) 1009
BpuMI CCSGG 1 cut(s) 79
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 6 cut(s) 78, 228, 349, 453, 555, 624
Bsc4I CCNNNNNNNGG 1 cut(s) 350
Bse1I ACTGG 5 cut(s) 72, 427, 480, 586, 951
Bse3DI GCAATG 1 cut(s) 514
BseBI CCWGG 2 cut(s) 351, 672
BseDI CCNNGG 6 cut(s) 78, 228, 349, 453, 555, 624
BseLI CCNNNNNNNGG 1 cut(s) 350
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 4 cut(s) 252, 398, 812, 926
BseNI ACTGG 5 cut(s) 72, 427, 480, 586, 951
BseXI GCAGC 1 cut(s) 749
BseYI CCCAGC 1 cut(s) 89
Bsh1236I CGCG 2 cut(s) 47, 173
BshFI GGCC 1 cut(s) 227
BshNI GGYRCC 1 cut(s) 551
BsiHKCI CYCGRG 3 cut(s) 454, 741, 869
BsiSI CCGG 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 350
BsmAI GTCTC 1 cut(s) 878
BsmI GAATGC 1 cut(s) 1005
BsnI GGCC 1 cut(s) 227
BsoBI CYCGRG 3 cut(s) 454, 741, 869
Bsp119I TTCGAA 1 cut(s) 1009
Bsp1286I GDGCHC 1 cut(s) 286
Bsp1407I TGTACA 1 cut(s) 706
Bsp143I GATC 4 cut(s) 32, 120, 931, 1117
Bsp19I CCATGG 1 cut(s) 228
BspACI CCGC 5 cut(s) 47, 168, 171, 319, 346
BspANI GGCC 1 cut(s) 227
BspCNI CTCAG 4 cut(s) 251, 397, 811, 927
BspFNI CGCG 2 cut(s) 47, 173
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 2 cut(s) 534, 553
BspMAI CTGCAG 1 cut(s) 739
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 1 cut(s) 115
BspQI GCTCTTC 1 cut(s) 843
BspT104I TTCGAA 1 cut(s) 1009
BspT107I GGYRCC 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 514
BsrGI TGTACA 1 cut(s) 706
BsrI ACTGG 5 cut(s) 72, 427, 480, 586, 951
BssECI CCNNGG 6 cut(s) 78, 228, 349, 453, 555, 624
BssMI GATC 4 cut(s) 32, 120, 931, 1117
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 2 cut(s) 228, 555
Bst2UI CCWGG 2 cut(s) 351, 672
Bst4CI ACNGT 4 cut(s) 619, 628, 1018, 1096
Bst6I CTCTTC 3 cut(s) 186, 843, 954
BstACI GRCGYC 1 cut(s) 135
BstAUI TGTACA 1 cut(s) 706
BstBI TTCGAA 1 cut(s) 1009
BstC8I GCNNGC 1 cut(s) 735
BstDEI CTNAG 4 cut(s) 238, 384, 798, 935
BstDSI CCRYGG 2 cut(s) 228, 624
BstFNI CGCG 2 cut(s) 47, 173
BstKTI GATC 4 cut(s) 35, 123, 934, 1120
BstMAI GTCTC 1 cut(s) 878
BstMBI GATC 4 cut(s) 32, 120, 931, 1117
BstMWI GCNNNNNNNGC 1 cut(s) 743
BstNI CCWGG 2 cut(s) 351, 672
BstNSI RCATGY 2 cut(s) 701, 1056
BstSCI CCNGG 3 cut(s) 77, 349, 670
BstSFI CTRYAG 2 cut(s) 272, 735
BstUI CGCG 2 cut(s) 47, 173
BstV1I GCAGC 1 cut(s) 749
BstX2I RGATCY 1 cut(s) 1117
BstYI RGATCY 1 cut(s) 1117
BsuI GTATCC 1 cut(s) 763
BsuRI GGCC 1 cut(s) 227
BtgI CCRYGG 2 cut(s) 228, 624
BtsIMutI CAGTG 1 cut(s) 420
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 1 cut(s) 735
CciI TCATGA 1 cut(s) 576
Cfr13I GGNCC 3 cut(s) 145, 363, 584
CseI GACGC 3 cut(s) 53, 143, 205
Csp6I GTAC 6 cut(s) 175, 310, 331, 707, 820, 996
CviAII CATG 9 cut(s) 97, 229, 482, 520, 572, 577, 641, 698, 1053
CviQI GTAC 6 cut(s) 175, 310, 331, 707, 820, 996
DdeI CTNAG 4 cut(s) 238, 384, 798, 935
DpnI GATC 4 cut(s) 34, 122, 933, 1119
DpnII GATC 4 cut(s) 32, 120, 931, 1117
DraIII CACNNNGTG 1 cut(s) 372
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 3 cut(s) 186, 843, 954
EarI CTCTTC 3 cut(s) 186, 843, 954
EciI GGCGGA 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 228, 555
Eco24I GRGCYC 1 cut(s) 286
Eco47I GGWCC 3 cut(s) 145, 363, 584
Eco57I CTGAAG 1 cut(s) 1106
Eco88I CYCGRG 3 cut(s) 454, 741, 869
EcoRII CCWGG 2 cut(s) 349, 670
EcoT14I CCWWGG 2 cut(s) 228, 555
EcoT38I GRGCYC 1 cut(s) 286
ErhI CCWWGG 2 cut(s) 228, 555
FaeI CATG 9 cut(s) 100, 232, 485, 523, 575, 580, 644, 701, 1056
FatI CATG 9 cut(s) 96, 228, 481, 519, 571, 576, 640, 697, 1052
FauI CCCGC 3 cut(s) 40, 326, 353
FauNDI CATATG 1 cut(s) 107
Fnu4HI GCNGC 2 cut(s) 171, 738
FriOI GRGCYC 1 cut(s) 286
Fsp4HI GCNGC 2 cut(s) 171, 738
FspBI CTAG 1 cut(s) 881
GluI GCNGC 2 cut(s) 171, 738
GsaI CCCAGC 1 cut(s) 93
HaeIII GGCC 1 cut(s) 227
HapII CCGG 1 cut(s) 78
HgaI GACGC 3 cut(s) 53, 143, 205
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 9 cut(s) 100, 232, 485, 523, 575, 580, 644, 701, 1056
HindIII AAGCTT 2 cut(s) 653, 761
HinfI GANTC 2 cut(s) 233, 568
HpaII CCGG 1 cut(s) 78
HphI GGTGA 2 cut(s) 130, 941
Hpy166II GTNNAC 4 cut(s) 220, 366, 624, 1072
Hpy188I TCNGA 6 cut(s) 64, 241, 450, 726, 936, 1130
Hpy188III TCNNGA 4 cut(s) 577, 871, 979, 1037
Hpy8I GTNNAC 4 cut(s) 220, 366, 624, 1072
Hpy99I CGWCG 2 cut(s) 47, 137
HpyAV CCTTC 1 cut(s) 677
HpyCH4III ACNGT 4 cut(s) 619, 628, 1018, 1096
HpyCH4V TGCA 6 cut(s) 4, 396, 408, 701, 737, 860
HpyF10VI GCNNNNNNNGC 1 cut(s) 743
HpyF3I CTNAG 4 cut(s) 238, 384, 798, 935
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 9 cut(s) 100, 232, 485, 523, 575, 580, 644, 701, 1056
Kzo9I GATC 4 cut(s) 32, 120, 931, 1117
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 3 cut(s) 264, 469, 1049
Lsp1109I GCAGC 1 cut(s) 749
MaeI CTAG 1 cut(s) 881
MaeIII GTNAC 1 cut(s) 953
MalI GATC 4 cut(s) 34, 122, 933, 1119
MboI GATC 4 cut(s) 32, 120, 931, 1117
MboII GAAGA 7 cut(s) 31, 173, 802, 860, 971, 1112, 1127
MfeI CAATTG 3 cut(s) 140, 924, 1083
MflI RGATCY 1 cut(s) 1117
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 8 cut(s) 140, 150, 612, 679, 714, 875, 924, 1083
MroXI GAANNNNTTC 2 cut(s) 567, 1119
MseI TTAA 4 cut(s) 438, 903, 918, 1103
MslI CAYNNNNRTG 1 cut(s) 524
MspI CCGG 1 cut(s) 78
MspR9I CCNGG 3 cut(s) 79, 351, 672
MunI CAATTG 3 cut(s) 140, 924, 1083
Mva1269I GAATGC 1 cut(s) 1005
MvaI CCWGG 2 cut(s) 351, 672
MvnI CGCG 2 cut(s) 47, 173
MwoI GCNNNNNNNGC 1 cut(s) 743
NciI CCSGG 1 cut(s) 79
NcoI CCATGG 1 cut(s) 228
NdeI CATATG 1 cut(s) 107
NdeII GATC 4 cut(s) 32, 120, 931, 1117
NlaIII CATG 9 cut(s) 100, 232, 485, 523, 575, 580, 644, 701, 1056
NlaIV GGNNCC 2 cut(s) 534, 553
NmeAIII GCCGAG 1 cut(s) 203
NspI RCATGY 2 cut(s) 701, 1056
NspV TTCGAA 1 cut(s) 1009
PaeR7I CTCGAG 1 cut(s) 741
PagI TCATGA 1 cut(s) 576
PaqCI CACCTGC 1 cut(s) 106
PciSI GCTCTTC 1 cut(s) 843
PctI GAATGC 1 cut(s) 1005
PdmI GAANNNNTTC 2 cut(s) 567, 1119
PfeI GAWTC 2 cut(s) 233, 568
PfoI TCCNGGA 1 cut(s) 670
PkrI GCNGC 2 cut(s) 172, 739
PsiI TTATAA 1 cut(s) 266
Psp6I CCWGG 2 cut(s) 349, 670
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 2 cut(s) 349, 670
PspN4I GGNNCC 2 cut(s) 534, 553
PspPI GGNCC 3 cut(s) 145, 363, 584
PspXI VCTCGAGB 1 cut(s) 741
PstI CTGCAG 1 cut(s) 739
PsuI RGATCY 1 cut(s) 1117
RsaI GTAC 6 cut(s) 176, 311, 332, 708, 821, 997
RsaNI GTAC 6 cut(s) 175, 310, 331, 707, 820, 996
RseI CAYNNNNRTG 1 cut(s) 524
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 4 cut(s) 438, 903, 918, 1103
SatI GCNGC 2 cut(s) 171, 738
Sau3AI GATC 4 cut(s) 32, 120, 931, 1117
Sau96I GGNCC 3 cut(s) 145, 363, 584
ScrFI CCNGG 3 cut(s) 79, 351, 672
SduI GDGCHC 1 cut(s) 286
SfcI CTRYAG 2 cut(s) 272, 735
Sfr274I CTCGAG 1 cut(s) 741
SfuI TTCGAA 1 cut(s) 1009
SinI GGWCC 3 cut(s) 145, 363, 584
SlaI CTCGAG 1 cut(s) 741
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 741
SmoI CTYRAG 1 cut(s) 741
Sse9I AATT 8 cut(s) 140, 150, 612, 679, 714, 875, 924, 1083
SsiI CCGC 5 cut(s) 47, 168, 171, 319, 346
SspMI CTAG 1 cut(s) 881
StyD4I CCNGG 3 cut(s) 77, 349, 670
StyI CCWWGG 2 cut(s) 228, 555
TaaI ACNGT 4 cut(s) 619, 628, 1018, 1096
TaqI TCGA 4 cut(s) 31, 42, 742, 1009
TasI AATT 8 cut(s) 140, 150, 612, 679, 714, 875, 924, 1083
TatI WGTACW 3 cut(s) 330, 706, 819
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 2 cut(s) 233, 568
Tru1I TTAA 4 cut(s) 438, 903, 918, 1103
Tru9I TTAA 4 cut(s) 438, 903, 918, 1103
TscAI CASTG 1 cut(s) 427
TseI GCWGC 1 cut(s) 737
TspDTI ATGAA 9 cut(s) 85, 470, 508, 560, 584, 629, 647, 802, 861
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 1 cut(s) 427
VpaK11BI GGWCC 3 cut(s) 145, 363, 584
XapI RAATTY 3 cut(s) 150, 679, 714
XceI RCATGY 2 cut(s) 701, 1056
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XhoI CTCGAG 1 cut(s) 741
XmnI GAANNNNTTC 2 cut(s) 567, 1119
XspI CTAG 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.