MD10G1105300.v1.1
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007961
Physical Location & Seq
Forward (+)
17231635 .. 17234043
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1105300.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGACTTCACAGCAGAGCCATAAGCCACACGCCGACTCCATTGCTGCAACACCCAAACCAACATTCAAAACTATGCAAGGTGCGCAAAATACGACGGCGTTTCGATCAATCTTCGACGCCGGAGAGCTGAATTCGGAACTGGAAAAGTCCGGGGTCAAGCCCAGCTTCATCCCCAACTTATGGAAGCAGGTGATCCAAAACCCTGATGCCCAATTCGACCAAATTCCATCTCTGCCCTCGGCAGCGTATCCTCTCCTAACCTCCAAATTCAAAACCCTAACCTCCTCTGTCCACAAGGTCATGGACTCCTCAGACCAACTCACCACCAAGCTCCTCATAAAACTGCAGAATGGGGCTCTTGTGGAAGCTGTGATTATGCGATACGACAGCCGCTTGGGGAGGTATGATGGGAAGCCCCGCCCTGGTGGTCCTAGGTCCACCTTGTGTGTATCATCTCAGGTTGGTTGCAAAATGGGTTGTACTTTCTGTGCTACTGGGAGTATGGGGTTTAAGAGCAATCTGACCTCGGGGGAGATTGTGGAGCAGTTGGTTCATGCTTCTCGCATTGCGCCTATACGCAATGTTGTGTTCATGGGAATGGGGGAACCTTTGAATAATTATGGTTCCTTGGTGGAAGCGGTTCATGTCATGACTGGATCACCATTTCACTTGTCACCTAAGAAAATTACTGTATCGACGGTTGGTATTATTCATGCTATCAAAAAGCTTCATAGTGATCTTCCAGGTCTGAATTTAGCAGTTTCACTGCATGCACCAGTCCAAGAAATCCGTTGTCAGATAATGCCTGCAGCTCGGGCTTTTCCTTTAGTAAAGCTTATGGATGCACTGCAAGAATATCAAAAGAACACTCAGCAGAAAATCTTCATTGAGTACATAATGCTTGATGGAGTGAATGATGAAGAGCACCATGCACACCAACTCGGAAAATTGCTAGAGACATTTCAAGTGGTAATTAATTTAATACCTTTTAATCCAATCGGTATTCTGAGTAAATTCACAACCAGTAACGAAAACAAAGTAACAAGATTCCAGAAAATTCTACGAGGCACTTATGGTATCCGAACGACAGTACGTAAGGAAATGGGTCAGGATATAAGTGGAGCATGTGGTCAGTTGGTAGTGAACTTACCCAACAAGAGGTCAACTGAGAACACTCTAACTGATATAGAAGATCTTCAGGTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

403

Amino Acids

44.25

Weight (kDa)

9.25

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 151 - 309 1.1e-11 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 178
Acc16I TGCGCA 1 cut(s) 84
Acc36I ACCTGC 1 cut(s) 178
AccB7I CCANNNNNTGG 1 cut(s) 180
AciI CCGC 3 cut(s) 391, 418, 638
AclWI GGATC 2 cut(s) 187, 664
AcsI RAATTY 6 cut(s) 130, 222, 266, 751, 1013, 1056
AcuI CTGAAG 1 cut(s) 1181
AcyI GRCGYC 1 cut(s) 117
AdeI CACNNNGTG 1 cut(s) 444
AfaI GTAC 3 cut(s) 481, 893, 1092
AfiI CCNNNNNNNGG 3 cut(s) 180, 422, 1158
AgsI TTSAA 4 cut(s) 67, 271, 613, 965
AjnI CCWGG 2 cut(s) 421, 742
AjuI GAANNNNNNNTTGG 2 cut(s) 47, 79
AluBI AGCT 7 cut(s) 127, 165, 331, 368, 727, 812, 835
AluI AGCT 7 cut(s) 127, 165, 331, 368, 727, 812, 835
Alw21I GWGCWC 1 cut(s) 927
AlwI GGATC 2 cut(s) 187, 664
Ama87I CYCGRG 2 cut(s) 526, 813
ApeKI GCWGC 3 cut(s) 44, 242, 809
ApoI RAATTY 6 cut(s) 130, 222, 266, 751, 1013, 1056
AseI ATTAAT 1 cut(s) 975
Asp700I GAANNNNTTC 3 cut(s) 639, 881, 1194
AspA2I CCTAGG 1 cut(s) 431
AspLEI GCGC 2 cut(s) 85, 571
AspS9I GGNCC 2 cut(s) 428, 435
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 4 cut(s) 202, 313, 651, 666
AvaI CYCGRG 2 cut(s) 526, 813
AvaII GGWCC 2 cut(s) 428, 435
AvrII CCTAGG 1 cut(s) 431
BanII GRGCYC 1 cut(s) 358
Bbv12I GWGCWC 1 cut(s) 927
BbvI GCAGC 3 cut(s) 31, 254, 821
BccI CCATC 3 cut(s) 235, 401, 899
BceAI ACGGC 1 cut(s) 111
BcgI CGANNNNNNTGC 2 cut(s) 23, 57
BciT130I CCWGG 2 cut(s) 423, 744
BciVI GTATCC 2 cut(s) 258, 1088
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 950
BfaI CTAG 2 cut(s) 432, 953
BfmI CTRYAG 2 cut(s) 344, 807
BfuAI ACCTGC 1 cut(s) 178
BfuI GTATCC 2 cut(s) 258, 1088
BglII AGATCT 1 cut(s) 1192
BisI GCNGC 4 cut(s) 45, 243, 391, 810
BlnI CCTAGG 1 cut(s) 431
BlsI GCNGC 4 cut(s) 46, 244, 392, 811
Bme1390I CCNGG 3 cut(s) 151, 423, 744
Bme18I GGWCC 2 cut(s) 428, 435
BmeT110I CYCGRG 2 cut(s) 526, 813
BmgT120I GGNCC 2 cut(s) 428, 435
BmiI GGNNCC 2 cut(s) 606, 625
BmrFI CCNGG 3 cut(s) 151, 423, 744
BmrI ACTGGG 1 cut(s) 504
BmsI GCATC 2 cut(s) 196, 832
BmuI ACTGGG 1 cut(s) 504
BpuMI CCSGG 1 cut(s) 151
BsaAI YACGTR 1 cut(s) 1094
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 6 cut(s) 150, 237, 421, 431, 525, 627
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 422, 1158
Bse1I ACTGG 5 cut(s) 144, 499, 658, 776, 1023
Bse3DI GCAATG 3 cut(s) 39, 564, 586
BseBI CCWGG 2 cut(s) 423, 744
BseDI CCNNGG 6 cut(s) 150, 237, 421, 431, 525, 627
BseGI GGATG 2 cut(s) 168, 847
BseLI CCNNNNNNNGG 3 cut(s) 180, 422, 1158
BseMI GCAATG 3 cut(s) 39, 564, 586
BseMII CTCAG 5 cut(s) 324, 470, 884, 998, 1158
BseNI ACTGG 5 cut(s) 144, 499, 658, 776, 1023
BseRI GAGGAG 3 cut(s) 274, 298, 323
BseXI GCAGC 3 cut(s) 31, 254, 821
BseYI CCCAGC 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 927
BsiHKCI CYCGRG 2 cut(s) 526, 813
BsiSI CCGG 2 cut(s) 120, 150
BslI CCNNNNNNNGG 3 cut(s) 180, 422, 1158
BsmAI GTCTC 1 cut(s) 950
BsoBI CYCGRG 2 cut(s) 526, 813
Bsp1286I GDGCHC 2 cut(s) 358, 927
Bsp143I GATC 5 cut(s) 104, 192, 656, 736, 1192
BspACI CCGC 3 cut(s) 391, 418, 638
BspCNI CTCAG 5 cut(s) 323, 469, 883, 999, 1159
BspHI TCATGA 1 cut(s) 648
BspLI GGNNCC 2 cut(s) 606, 625
BspMAI CTGCAG 2 cut(s) 348, 811
BspMI ACCTGC 1 cut(s) 178
BspPI GGATC 2 cut(s) 187, 664
BspQI GCTCTTC 1 cut(s) 915
BsrDI GCAATG 3 cut(s) 39, 564, 586
BsrI ACTGG 5 cut(s) 144, 499, 658, 776, 1023
BssECI CCNNGG 6 cut(s) 150, 237, 421, 431, 525, 627
BssMI GATC 5 cut(s) 104, 192, 656, 736, 1192
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 2 cut(s) 431, 627
Bst2UI CCWGG 2 cut(s) 423, 744
Bst4CI ACNGT 3 cut(s) 691, 700, 1090
Bst6I CTCTTC 1 cut(s) 915
BstACI GRCGYC 1 cut(s) 117
BstBAI YACGTR 1 cut(s) 1094
BstC8I GCNNGC 2 cut(s) 771, 807
BstDEI CTNAG 6 cut(s) 310, 456, 678, 870, 1007, 1167
BstF5I GGATG 2 cut(s) 168, 847
BstHHI GCGC 2 cut(s) 85, 571
BstKTI GATC 5 cut(s) 107, 195, 659, 739, 1195
BstMAI GTCTC 1 cut(s) 950
BstMBI GATC 5 cut(s) 104, 192, 656, 736, 1192
BstMWI GCNNNNNNNGC 2 cut(s) 82, 815
BstNI CCWGG 2 cut(s) 423, 744
BstNSI RCATGY 2 cut(s) 773, 1128
BstSCI CCNGG 3 cut(s) 149, 421, 742
BstSFI CTRYAG 2 cut(s) 344, 807
BstSNI TACGTA 1 cut(s) 1094
BstV1I GCAGC 3 cut(s) 31, 254, 821
BstX2I RGATCY 1 cut(s) 1192
BstYI RGATCY 1 cut(s) 1192
BsuI GTATCC 2 cut(s) 258, 1088
BtsCI GGATG 2 cut(s) 168, 847
BtsI GCAGTG 2 cut(s) 764, 845
BtsIMutI CAGTG 2 cut(s) 764, 845
BveI ACCTGC 1 cut(s) 178
Cac8I GCNNGC 2 cut(s) 771, 807
CciI TCATGA 1 cut(s) 648
CfoI GCGC 2 cut(s) 85, 571
Cfr13I GGNCC 2 cut(s) 428, 435
CseI GACGC 1 cut(s) 125
Csp6I GTAC 3 cut(s) 480, 892, 1091
CviAII CATG 9 cut(s) 301, 554, 592, 644, 649, 713, 770, 929, 1125
CviQI GTAC 3 cut(s) 480, 892, 1091
DdeI CTNAG 6 cut(s) 310, 456, 678, 870, 1007, 1167
DpnI GATC 5 cut(s) 106, 194, 658, 738, 1194
DpnII GATC 5 cut(s) 104, 192, 656, 736, 1192
DraIII CACNNNGTG 1 cut(s) 444
Eam1104I CTCTTC 1 cut(s) 915
EarI CTCTTC 1 cut(s) 915
Eco105I TACGTA 1 cut(s) 1094
Eco130I CCWWGG 2 cut(s) 431, 627
Eco24I GRGCYC 1 cut(s) 358
Eco47I GGWCC 2 cut(s) 428, 435
Eco57I CTGAAG 1 cut(s) 1181
Eco88I CYCGRG 2 cut(s) 526, 813
EcoRI GAATTC 1 cut(s) 130
EcoRII CCWGG 2 cut(s) 421, 742
EcoT14I CCWWGG 2 cut(s) 431, 627
EcoT38I GRGCYC 1 cut(s) 358
ErhI CCWWGG 2 cut(s) 431, 627
FaeI CATG 9 cut(s) 304, 557, 595, 647, 652, 716, 773, 932, 1128
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FatI CATG 9 cut(s) 300, 553, 591, 643, 648, 712, 769, 928, 1124
FauI CCCGC 1 cut(s) 425
Fnu4HI GCNGC 4 cut(s) 45, 243, 391, 810
FokI GGATG 2 cut(s) 155, 854
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 4 cut(s) 45, 243, 391, 810
FspBI CTAG 2 cut(s) 432, 953
FspI TGCGCA 1 cut(s) 84
GlaI GCGC 2 cut(s) 84, 570
GluI GCNGC 4 cut(s) 45, 243, 391, 810
GsaI CCCAGC 1 cut(s) 165
HapII CCGG 2 cut(s) 120, 150
HgaI GACGC 1 cut(s) 125
HhaI GCGC 2 cut(s) 85, 571
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 9 cut(s) 304, 557, 595, 647, 652, 716, 773, 932, 1128
Hin6I GCGC 2 cut(s) 83, 569
HinP1I GCGC 2 cut(s) 83, 569
HincII GTYRAC 1 cut(s) 1164
HindII GTYRAC 1 cut(s) 1164
HindIII AAGCTT 2 cut(s) 725, 833
HinfI GANTC 3 cut(s) 35, 305, 1047
HpaII CCGG 2 cut(s) 120, 150
HphI GGTGA 4 cut(s) 202, 313, 651, 666
Hpy166II GTNNAC 4 cut(s) 292, 438, 1144, 1164
Hpy188I TCNGA 9 cut(s) 136, 313, 522, 750, 798, 944, 1008, 1082, 1205
Hpy188III TCNNGA 3 cut(s) 649, 1051, 1109
Hpy8I GTNNAC 4 cut(s) 292, 438, 1144, 1164
Hpy99I CGWCG 3 cut(s) 97, 119, 700
HpyCH4III ACNGT 3 cut(s) 691, 700, 1090
HpyCH4IV ACGT 1 cut(s) 1093
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 815
HpyF3I CTNAG 6 cut(s) 310, 456, 678, 870, 1007, 1167
HpySE526I ACGT 1 cut(s) 1093
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 9 cut(s) 304, 557, 595, 647, 652, 716, 773, 932, 1128
HspAI GCGC 2 cut(s) 83, 569
Kzo9I GATC 5 cut(s) 104, 192, 656, 736, 1192
LguI GCTCTTC 1 cut(s) 915
LmnI GCTCC 3 cut(s) 336, 541, 1121
Lsp1109I GCAGC 3 cut(s) 31, 254, 821
LweI GCATC 2 cut(s) 196, 832
MaeI CTAG 2 cut(s) 432, 953
MaeII ACGT 1 cut(s) 1093
MaeIII GTNAC 3 cut(s) 672, 1025, 1039
MalI GATC 5 cut(s) 106, 194, 658, 738, 1194
MboI GATC 5 cut(s) 104, 192, 656, 736, 1192
MboII GAAGA 6 cut(s) 103, 731, 874, 932, 1187, 1202
MflI RGATCY 1 cut(s) 1192
MhlI GDGCHC 2 cut(s) 358, 927
MlyI GAGTC 2 cut(s) 29, 299
MroXI GAANNNNTTC 3 cut(s) 639, 881, 1194
MseI TTAA 4 cut(s) 510, 975, 980, 990
MslI CAYNNNNRTG 1 cut(s) 596
MspI CCGG 2 cut(s) 120, 150
MspR9I CCNGG 3 cut(s) 151, 423, 744
MvaI CCWGG 2 cut(s) 423, 744
MwoI GCNNNNNNNGC 2 cut(s) 82, 815
NciI CCSGG 1 cut(s) 151
NdeII GATC 5 cut(s) 104, 192, 656, 736, 1192
NlaIII CATG 9 cut(s) 304, 557, 595, 647, 652, 716, 773, 932, 1128
NlaIV GGNNCC 2 cut(s) 606, 625
NmeAIII GCCGAG 1 cut(s) 218
NmuCI GTSAC 1 cut(s) 672
NsbI TGCGCA 1 cut(s) 84
NspI RCATGY 2 cut(s) 773, 1128
PaeI GCATGC 1 cut(s) 773
PagI TCATGA 1 cut(s) 648
PaqCI CACCTGC 1 cut(s) 178
PciSI GCTCTTC 1 cut(s) 915
PdmI GAANNNNTTC 3 cut(s) 639, 881, 1194
PfeI GAWTC 1 cut(s) 1047
PflMI CCANNNNNTGG 1 cut(s) 180
PkrI GCNGC 4 cut(s) 46, 244, 392, 811
PleI GAGTC 2 cut(s) 29, 299
PpsI GAGTC 2 cut(s) 29, 299
Ppu21I YACGTR 1 cut(s) 1094
PshBI ATTAAT 1 cut(s) 975
Psp6I CCWGG 2 cut(s) 421, 742
PspFI CCCAGC 1 cut(s) 161
PspGI CCWGG 2 cut(s) 421, 742
PspN4I GGNNCC 2 cut(s) 606, 625
PspPI GGNCC 2 cut(s) 428, 435
PstI CTGCAG 2 cut(s) 348, 811
PsuI RGATCY 1 cut(s) 1192
RsaI GTAC 3 cut(s) 481, 893, 1092
RsaNI GTAC 3 cut(s) 480, 892, 1091
RseI CAYNNNNRTG 1 cut(s) 596
SapI GCTCTTC 1 cut(s) 915
SaqAI TTAA 4 cut(s) 510, 975, 980, 990
SatI GCNGC 4 cut(s) 45, 243, 391, 810
Sau3AI GATC 5 cut(s) 104, 192, 656, 736, 1192
Sau96I GGNCC 2 cut(s) 428, 435
SchI GAGTC 2 cut(s) 29, 299
ScrFI CCNGG 3 cut(s) 151, 423, 744
SduI GDGCHC 2 cut(s) 358, 927
SfaNI GCATC 2 cut(s) 196, 832
SfcI CTRYAG 2 cut(s) 344, 807
SinI GGWCC 2 cut(s) 428, 435
SmiMI CAYNNNNRTG 1 cut(s) 596
SnaBI TACGTA 1 cut(s) 1094
SphI GCATGC 1 cut(s) 773
SsiI CCGC 3 cut(s) 391, 418, 638
SspMI CTAG 2 cut(s) 432, 953
StyD4I CCNGG 3 cut(s) 149, 421, 742
StyI CCWWGG 2 cut(s) 431, 627
TaaI ACNGT 3 cut(s) 691, 700, 1090
TaiI ACGT 1 cut(s) 1096
TaqI TCGA 4 cut(s) 103, 114, 216, 695
TatI WGTACW 2 cut(s) 479, 891
TauI GCSGC 1 cut(s) 393
TfiI GAWTC 1 cut(s) 1047
Tru1I TTAA 4 cut(s) 510, 975, 980, 990
Tru9I TTAA 4 cut(s) 510, 975, 980, 990
TscAI CASTG 2 cut(s) 771, 852
TseFI GTSAC 1 cut(s) 672
TseI GCWGC 3 cut(s) 44, 242, 809
Tsp45I GTSAC 1 cut(s) 672
TspDTI ATGAA 8 cut(s) 157, 542, 580, 632, 701, 719, 874, 933
TspGWI ACGGA 1 cut(s) 779
TspRI CASTG 2 cut(s) 771, 852
Van91I CCANNNNNTGG 1 cut(s) 180
VpaK11BI GGWCC 2 cut(s) 428, 435
VspI ATTAAT 1 cut(s) 975
XapI RAATTY 6 cut(s) 130, 222, 266, 751, 1013, 1056
XceI RCATGY 2 cut(s) 773, 1128
XmaJI CCTAGG 1 cut(s) 431
XmnI GAANNNNTTC 3 cut(s) 639, 881, 1194
XspI CTAG 2 cut(s) 432, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.