AT3G20790

Oxidoreductase family, NAD-binding Rossmann fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011989
Physical Location & Seq
Forward (+)
7268714 .. 7271287
2574 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20790.1

Sequence Viewer

Length: 1068 bp
ATGGTGAATCCTTCTTCACCTGGAATTGCCATACTCGGAGCTGGGATCTTCGTCAAAACTCAATACATTCCGAGATTAGCTGAGATCTCCGATCTCGTCGACCTGAAAGCGATTTGGAGCCGTACTGAGGAATCTGCAAAAGGAGCTGTGGAGATAGCAAGAAAGCATTTTCCTGAAGTGAAGTGCAAATGGGGAGATGAGGGTCTCAATGAGATCATTCAAGACAGTTCAATAGTTGGTGTTGCTGTAGTTGTAGCTGCAGAAACCATGGTTGAATTGTCACTGAAGATGCTCAAGGCAGGAAAGCACGTCCTTCAAGAGAAGCCAGCTGCTGCTTCAATCAGTGAGATAGAAACTGCAATGTCAAGCTATAGAAATATTTCTGCTGATTCCCCATGCCGTCCAATTTGGGCTGTGGCTGAAAATTATCGTTTTGAGCCAGCTTTTGTTGAGTTGAAGAAACTGATAGCAGAAATTGGAGATATGATGAACGTCCAGCTTATTATTGAGGGATCAATGAACAGTTCTAATCCTTATTTCTCAAGCTCCTGGAGGCGAAATTTGAGTGGTGGTTTCATCTTGGATATGGGTGTACATTACATTGCAGGATTAAGAATGCTTGTTGGATGTGAGGTAACATCAGTCTCAGCCACCACTACCCATGTGGATAAGACTTTACCTGGACCAGACAATATAACATCAAACTTTCAGCTCGAGAACGGATGCTCTGGGGTTTTTGTTATGGTTGTGTCTTCAAGGTCTCCAAAGATACTATGGAGAATCGTAGGACTAAAGGGCACTGTTCAACTAGAGCGTGGAGTGGAAGGTGGCCGACATGGTTACATGGCCACGATTTACGGGGAAGGAGGGACATCAAGGACCATCTTTTACCCATTCAGCGGAGTGACTGAAGAGTTAAAAGCGTTCTTCAATGATATTTCAGAAACTTCAAAGGAGCAAGAGCCTCGTCTATCATATGTGGAAGGTGCTCGTGACGTTGCAGTTCTTGAAGCAATGCTCGAGTCTGGTGCGAAAAATGGAGCCGTAGTCCCTGTCAACAAATTCTAG

Protein Analysis

355

Amino Acids

38.58

Weight (kDa)

5.57

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 9 - 120 3.7e-12 Oxidoreductase family, NAD-binding Rossmann fold
GFO_IDH_MocA_C3 PF22725 150 - 268 4.7e-15 GFO/IDH/MocA C-terminal domain
GFO_IDH_MocA_C PF02894 153 - 347 2.2e-12 Oxidoreductase family, C-terminal alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20790
fragaria_vesca FvH4_3g43551 FvH4_3g43551
malus_domestica MD11G1022800.v1.1
prunus_persica Prupe.6G018500_v2.0.a1
pyrus_communis pycom11g01860
rosa_chinensis RchiOBHm_Chr5g0078111
rosa_laevigata RLG00000031428 RLG00000036752
rosa_multiflora Rmu_sc0019972.1_g000004
rosa_roxburghii Rroxscaffold_1G00000370 Rroxscaffold_1G00004110
rosa_rugosa Rorug05G0458500 Rorug05G0458600
rosa_samantha Rh5AG052300 Rh5AG513200 Rh5CG559000 Rh5DG119600
rosa_wichuraiana Rw5G047600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 99
AciI CCGC 1 cut(s) 900
AclWI GGATC 2 cut(s) 53, 520
AcoI YGGCCR 2 cut(s) 829, 846
AcsI RAATTY 2 cut(s) 559, 1061
AcuI CTGAAG 3 cut(s) 195, 305, 930
AfaI GTAC 2 cut(s) 124, 594
AfiI CCNNNNNNNGG 2 cut(s) 127, 899
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 3 cut(s) 19, 548, 679
Alw21I GWGCWC 1 cut(s) 991
Alw26I GTCTC 3 cut(s) 209, 649, 765
AlwI GGATC 2 cut(s) 53, 520
AlwNI CAGNNNCTG 1 cut(s) 332
Ama87I CYCGRG 2 cut(s) 713, 1019
AoxI GGCC 2 cut(s) 829, 846
ApeKI GCWGC 3 cut(s) 257, 329, 332
ApoI RAATTY 2 cut(s) 559, 1061
Asp700I GAANNNNTTC 1 cut(s) 379
AspS9I GGNCC 2 cut(s) 683, 879
AsuHPI GGTGA 2 cut(s) 9, 16
AvaI CYCGRG 2 cut(s) 713, 1019
AvaII GGWCC 2 cut(s) 683, 879
BaeGI GKGCMC 1 cut(s) 800
BalI TGGCCA 1 cut(s) 848
BauI CACGAG 1 cut(s) 990
BbsI GAAGAC 1 cut(s) 744
Bbv12I GWGCWC 1 cut(s) 991
BbvI GCAGC 3 cut(s) 244, 316, 319
BccI CCATC 1 cut(s) 890
BceAI ACGGC 3 cut(s) 105, 384, 1028
BcgI CGANNNNNNTGC 4 cut(s) 947, 981, 1010, 1044
BciT130I CCWGG 3 cut(s) 21, 550, 681
BcoDI GTCTC 3 cut(s) 209, 649, 765
BfaI CTAG 2 cut(s) 809, 1066
BfmI CTRYAG 3 cut(s) 246, 258, 370
BglII AGATCT 1 cut(s) 84
BisI GCNGC 3 cut(s) 258, 330, 333
BlsI GCNGC 3 cut(s) 259, 331, 334
Bme1390I CCNGG 3 cut(s) 21, 550, 681
Bme18I GGWCC 2 cut(s) 683, 879
BmeT110I CYCGRG 2 cut(s) 713, 1019
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 2 cut(s) 683, 879
BmiI GGNNCC 2 cut(s) 119, 1042
BmrFI CCNGG 3 cut(s) 21, 550, 681
BmsI GCATC 2 cut(s) 279, 713
BpiI GAAGAC 1 cut(s) 744
BpmI CTGGAG 1 cut(s) 571
BpuEI CTTGAG 2 cut(s) 278, 526
BsaI GGTCTC 2 cut(s) 209, 765
BsaJI CCNNGG 1 cut(s) 267
BsaXI ACNNNNNCTCC 2 cut(s) 1032, 1062
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 899
Bse3DI GCAATG 3 cut(s) 366, 600, 1020
BseBI CCWGG 3 cut(s) 21, 550, 681
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 2 cut(s) 632, 728
BseLI CCNNNNNNNGG 2 cut(s) 127, 899
BseMI GCAATG 3 cut(s) 366, 600, 1020
BseMII CTCAG 3 cut(s) 72, 117, 660
BseSI GKGCMC 1 cut(s) 800
BseXI GCAGC 3 cut(s) 244, 316, 319
BseYI CCCAGC 1 cut(s) 41
BshFI GGCC 2 cut(s) 831, 848
BsiHKAI GWGCWC 1 cut(s) 991
BsiHKCI CYCGRG 2 cut(s) 713, 1019
BslFI GGGAC 2 cut(s) 883, 1034
BslI CCNNNNNNNGG 2 cut(s) 127, 899
BsmAI GTCTC 3 cut(s) 209, 649, 765
BsmFI GGGAC 2 cut(s) 883, 1034
BsmI GAATGC 1 cut(s) 621
BsnI GGCC 2 cut(s) 831, 848
Bso31I GGTCTC 2 cut(s) 209, 765
BsoBI CYCGRG 2 cut(s) 713, 1019
Bsp1286I GDGCHC 2 cut(s) 800, 991
Bsp1407I TGTACA 1 cut(s) 592
Bsp143I GATC 5 cut(s) 45, 84, 91, 213, 512
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 1 cut(s) 900
BspANI GGCC 2 cut(s) 831, 848
BspCNI CTCAG 3 cut(s) 73, 118, 659
BspLI GGNNCC 2 cut(s) 119, 1042
BspMAI CTGCAG 1 cut(s) 262
BspPI GGATC 2 cut(s) 53, 520
BspTNI GGTCTC 2 cut(s) 209, 765
BsrDI GCAATG 3 cut(s) 366, 600, 1020
BsrGI TGTACA 1 cut(s) 592
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 5 cut(s) 45, 84, 91, 213, 512
BssSI CACGAG 1 cut(s) 990
BssT1I CCWWGG 1 cut(s) 267
Bst2BI CACGAG 1 cut(s) 990
Bst2UI CCWGG 3 cut(s) 21, 550, 681
Bst4CI ACNGT 3 cut(s) 227, 524, 802
Bst6I CTCTTC 1 cut(s) 906
BstAUI TGTACA 1 cut(s) 592
BstC8I GCNNGC 2 cut(s) 327, 441
BstDEI CTNAG 3 cut(s) 81, 126, 646
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 2 cut(s) 632, 728
BstKTI GATC 5 cut(s) 48, 87, 94, 216, 515
BstMAI GTCTC 3 cut(s) 209, 649, 765
BstMBI GATC 5 cut(s) 45, 84, 91, 213, 512
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNI CCWGG 3 cut(s) 21, 550, 681
BstSCI CCNGG 3 cut(s) 19, 548, 679
BstSFI CTRYAG 3 cut(s) 246, 258, 370
BstSLI GKGCMC 1 cut(s) 800
BstV1I GCAGC 3 cut(s) 244, 316, 319
BstV2I GAAGAC 1 cut(s) 744
BstX2I RGATCY 2 cut(s) 45, 84
BstYI RGATCY 2 cut(s) 45, 84
BsuRI GGCC 2 cut(s) 831, 848
BtgI CCRYGG 1 cut(s) 267
BtrI CACGTC 1 cut(s) 310
BtsCI GGATG 2 cut(s) 632, 728
BtsIMutI CAGTG 3 cut(s) 281, 349, 798
Cac8I GCNNGC 2 cut(s) 327, 441
CaiI CAGNNNCTG 1 cut(s) 332
Cfr13I GGNCC 2 cut(s) 683, 879
Csp6I GTAC 2 cut(s) 123, 593
CviAII CATG 5 cut(s) 268, 396, 662, 836, 844
CviQI GTAC 2 cut(s) 123, 593
DdeI CTNAG 3 cut(s) 81, 126, 646
DpnI GATC 5 cut(s) 47, 86, 93, 215, 514
DpnII GATC 5 cut(s) 45, 84, 91, 213, 512
EaeI YGGCCR 2 cut(s) 829, 846
Eam1104I CTCTTC 1 cut(s) 906
EarI CTCTTC 1 cut(s) 906
Eco130I CCWWGG 1 cut(s) 267
Eco31I GGTCTC 2 cut(s) 209, 765
Eco47I GGWCC 2 cut(s) 683, 879
Eco57I CTGAAG 3 cut(s) 195, 305, 930
Eco88I CYCGRG 2 cut(s) 713, 1019
EcoRII CCWGG 3 cut(s) 19, 548, 679
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 5 cut(s) 271, 399, 665, 839, 847
FaqI GGGAC 2 cut(s) 883, 1034
FatI CATG 5 cut(s) 267, 395, 661, 835, 843
FauNDI CATATG 1 cut(s) 976
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 3 cut(s) 258, 330, 333
FokI GGATG 2 cut(s) 639, 735
Fsp4HI GCNGC 3 cut(s) 258, 330, 333
FspBI CTAG 2 cut(s) 809, 1066
GluI GCNGC 3 cut(s) 258, 330, 333
GsaI CCCAGC 1 cut(s) 45
GsuI CTGGAG 1 cut(s) 571
HaeIII GGCC 2 cut(s) 831, 848
Hin1II CATG 5 cut(s) 271, 399, 665, 839, 847
HincII GTYRAC 2 cut(s) 100, 1057
HindII GTYRAC 2 cut(s) 100, 1057
HinfI GANTC 5 cut(s) 7, 131, 389, 780, 1022
HphI GGTGA 2 cut(s) 9, 16
Hpy166II GTNNAC 3 cut(s) 100, 593, 1057
Hpy188I TCNGA 4 cut(s) 38, 72, 91, 943
Hpy188III TCNNGA 6 cut(s) 173, 221, 317, 715, 992, 1007
Hpy8I GTNNAC 3 cut(s) 100, 593, 1057
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 5 cut(s) 21, 323, 818, 857, 977
HpyCH4III ACNGT 3 cut(s) 227, 524, 802
HpyCH4IV ACGT 3 cut(s) 309, 492, 996
HpyCH4V TGCA 6 cut(s) 137, 186, 260, 359, 605, 1001
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 3 cut(s) 81, 126, 646
HpySE526I ACGT 3 cut(s) 309, 492, 996
Hsp92II CATG 5 cut(s) 271, 399, 665, 839, 847
Kzo9I GATC 5 cut(s) 45, 84, 91, 213, 512
LmnI GCTCC 6 cut(s) 38, 117, 143, 551, 955, 1040
Lsp1109I GCAGC 3 cut(s) 244, 316, 319
LweI GCATC 2 cut(s) 279, 713
MaeI CTAG 2 cut(s) 809, 1066
MaeII ACGT 3 cut(s) 309, 492, 996
MaeIII GTNAC 5 cut(s) 279, 634, 839, 904, 992
MalI GATC 5 cut(s) 47, 86, 93, 215, 514
MboI GATC 5 cut(s) 45, 84, 91, 213, 512
MboII GAAGA 7 cut(s) 6, 40, 298, 469, 744, 919, 923
MflI RGATCY 2 cut(s) 45, 84
MhlI GDGCHC 2 cut(s) 800, 991
MlsI TGGCCA 1 cut(s) 848
MluCI AATT 7 cut(s) 24, 275, 405, 424, 474, 559, 1061
MluNI TGGCCA 1 cut(s) 848
MlyI GAGTC 1 cut(s) 1031
MmeI TCCRAC 1 cut(s) 604
MnlI CCTC 7 cut(s) 121, 193, 502, 546, 625, 860, 975
Mox20I TGGCCA 1 cut(s) 848
MroXI GAANNNNTTC 1 cut(s) 379
MscI TGGCCA 1 cut(s) 848
MseI TTAA 2 cut(s) 611, 917
Msp20I TGGCCA 1 cut(s) 848
MspA1I CMGCKG 2 cut(s) 329, 900
MspR9I CCNGG 3 cut(s) 21, 550, 681
Mva1269I GAATGC 1 cut(s) 621
MvaI CCWGG 3 cut(s) 21, 550, 681
MwoI GCNNNNNNNGC 1 cut(s) 143
NcoI CCATGG 1 cut(s) 267
NdeI CATATG 1 cut(s) 976
NdeII GATC 5 cut(s) 45, 84, 91, 213, 512
NlaIII CATG 5 cut(s) 271, 399, 665, 839, 847
NlaIV GGNNCC 2 cut(s) 119, 1042
NmuCI GTSAC 3 cut(s) 279, 904, 992
PaeR7I CTCGAG 2 cut(s) 713, 1019
PctI GAATGC 1 cut(s) 621
PdmI GAANNNNTTC 1 cut(s) 379
PfeI GAWTC 4 cut(s) 7, 131, 389, 780
PfoI TCCNGGA 1 cut(s) 548
PkrI GCNGC 3 cut(s) 259, 331, 334
PleI GAGTC 1 cut(s) 1030
PpsI GAGTC 1 cut(s) 1030
Psp6I CCWGG 3 cut(s) 19, 548, 679
PspFI CCCAGC 1 cut(s) 41
PspGI CCWGG 3 cut(s) 19, 548, 679
PspN4I GGNNCC 2 cut(s) 119, 1042
PspPI GGNCC 2 cut(s) 683, 879
PspXI VCTCGAGB 1 cut(s) 1019
PstI CTGCAG 1 cut(s) 262
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 2 cut(s) 45, 84
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 2 cut(s) 124, 594
RsaNI GTAC 2 cut(s) 123, 593
SalI GTCGAC 1 cut(s) 98
SaqAI TTAA 2 cut(s) 611, 917
SatI GCNGC 3 cut(s) 258, 330, 333
Sau3AI GATC 5 cut(s) 45, 84, 91, 213, 512
Sau96I GGNCC 2 cut(s) 683, 879
SchI GAGTC 1 cut(s) 1031
ScrFI CCNGG 3 cut(s) 21, 550, 681
SduI GDGCHC 2 cut(s) 800, 991
SfaNI GCATC 2 cut(s) 279, 713
SfcI CTRYAG 3 cut(s) 246, 258, 370
Sfr274I CTCGAG 2 cut(s) 713, 1019
SinI GGWCC 2 cut(s) 683, 879
SlaI CTCGAG 2 cut(s) 713, 1019
SmlI CTYRAG 4 cut(s) 293, 541, 713, 1019
SmoI CTYRAG 4 cut(s) 293, 541, 713, 1019
Sse9I AATT 7 cut(s) 24, 275, 405, 424, 474, 559, 1061
SsiI CCGC 1 cut(s) 900
SspI AATATT 1 cut(s) 379
SspMI CTAG 2 cut(s) 809, 1066
StyD4I CCNGG 3 cut(s) 19, 548, 679
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 3 cut(s) 227, 524, 802
TaiI ACGT 3 cut(s) 312, 495, 999
TaqI TCGA 3 cut(s) 99, 714, 1020
TasI AATT 7 cut(s) 24, 275, 405, 424, 474, 559, 1061
TatI WGTACW 1 cut(s) 592
TfiI GAWTC 4 cut(s) 7, 131, 389, 780
Tru1I TTAA 2 cut(s) 611, 917
Tru9I TTAA 2 cut(s) 611, 917
TscAI CASTG 3 cut(s) 288, 349, 805
TseFI GTSAC 3 cut(s) 279, 904, 992
TseI GCWGC 3 cut(s) 257, 329, 332
Tsp45I GTSAC 3 cut(s) 279, 904, 992
TspDTI ATGAA 3 cut(s) 503, 533, 565
TspGWI ACGGA 1 cut(s) 735
TspRI CASTG 3 cut(s) 288, 349, 805
VpaK11BI GGWCC 2 cut(s) 683, 879
XapI RAATTY 2 cut(s) 559, 1061
XcmI CCANNNNNNNNNTGG 2 cut(s) 661, 771
XhoI CTCGAG 2 cut(s) 713, 1019
XmiI GTMKAC 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 379
XspI CTAG 2 cut(s) 809, 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.