Rh5AG513200

Oxidoreductase family, C-terminal alpha/beta domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011989
Physical Location & Seq
Forward (+)
86777332 .. 86781270
3939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG513200.1

Sequence Viewer

Length: 1029 bp
ATGGCAGAAGCACCCCAGATTGCCATTCTTGGAGCTGGTACTTTTGTGAAGACCCAATACATTCCAAGGCTGGCTGAGATCTCTAACCTTGTCAACCTTAAAGCCATTTGGAGCCGTTCTGAGGAATCTGCTAGAGGTGCAGTTGAGATTGCTGGGAAACATTTTCCAGGAGTAGAATGGAAATGGGGTGATCATGGTCTTGAGGAAATTATTGCAGATAGTTCAATTCTTGGTGTTGCTGTGGTTTTAGCTGGCCAAGCTCAGGTTGATTTCTCACTTAAGCTTCTCAAAGCAGGGAAGAATGTCCTTCAAGAGAAACCAGCAGCAGCTACTACAAGTGAATTGGAAACTGCAGTGTCAAGTTATAAATCTATTGTTGCTAATACTCCCAATAAACCAATCTGGGCTGTAGCAGAAAATTATCGATTTGAACCTGCTTTTGTTGAGGGCAAGAAACTAATGACTGAAATTGGAGATGTGATGAGCATCCAAGTTATTGTTGAAGGATCAATGAACAGTTCAAACCCTTACTTCTCAAGCTCTTGGAGGCGAGACTTTAATCTTGCTGGATGTGAGATAGTCTCGGTGTCAGCTACAACATCTCATGTCGACAAGACTTTACCTCCCCCAGATAACATATCCTCTCTCTTTCAACTGGAGAATGGCTGTTCTGGGATTTTTGTGATGGTGGTCTCCTCAAGGTCTCCCAAGATAGTTTGGCGATTTGTTGGCTTGAAGGGAACACTGGAAGTTGAGCGTGGAAACCAAGATGGACGACACGGCTATGTGGTCACATTTTATAGTGCTGATGGACAAAGGAAAAGCTCCTTCTACCAATTTAGAGGAGTGACTGAAGAATTCATTGCTTTTATTAATGACATAAAGCAAGCCACTTTAAAGAAGGGAGCTGGCTATGAAGCTGAGCCTCGCATGTCATTTCTGGAAGGTGCTAGAGATGTTGCTGTTCTAGAGGCAATGCTCGAATCTGGAGGAAAGCAAGGGGCACCAGTCCATGTGAAGAAATTTTGA

Protein Analysis

342

Amino Acids

37.25

Weight (kDa)

6.27

Isoelectric Point (pI)

33.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 7 - 120 1.8e-11 Oxidoreductase family, NAD-binding Rossmann fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20790
fragaria_vesca FvH4_3g43551 FvH4_3g43551
malus_domestica MD11G1022800.v1.1
prunus_persica Prupe.6G018500_v2.0.a1
pyrus_communis pycom11g01860
rosa_chinensis RchiOBHm_Chr5g0078111
rosa_laevigata RLG00000031428 RLG00000036752
rosa_multiflora Rmu_sc0019972.1_g000004
rosa_roxburghii Rroxscaffold_1G00000370 Rroxscaffold_1G00004110
rosa_rugosa Rorug05G0458500 Rorug05G0458600
rosa_samantha Rh5AG052300 Rh5AG513200 Rh5CG559000 Rh5DG119600
rosa_wichuraiana Rw5G047600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
Acc36I ACCTGC 1 cut(s) 442
AccB1I GGYRCC 1 cut(s) 1003
AccI GTMKAC 1 cut(s) 609
AclWI GGATC 1 cut(s) 514
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 2 cut(s) 857, 1022
AcuI CTGAAG 1 cut(s) 873
AfaI GTAC 1 cut(s) 40
AfiI CCNNNNNNNGG 2 cut(s) 121, 262
AflII CTTAAG 1 cut(s) 278
AgsI TTSAA 7 cut(s) 225, 311, 431, 503, 522, 653, 736
AjnI CCWGG 1 cut(s) 166
Alw26I GTCTC 4 cut(s) 546, 586, 697, 708
AlwI GGATC 1 cut(s) 514
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 2 cut(s) 323, 326
ApoI RAATTY 2 cut(s) 857, 1022
AseI ATTAAT 1 cut(s) 873
AsuHPI GGTGA 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 1006
BaeI ACNNNNGTAYC 2 cut(s) 30, 63
BalI TGGCCA 1 cut(s) 255
BanI GGYRCC 1 cut(s) 1003
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
BbsI GAAGAC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 335, 338
BccI CCATC 3 cut(s) 679, 764, 803
BceAI ACGGC 2 cut(s) 99, 796
BciT130I CCWGG 1 cut(s) 168
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 4 cut(s) 546, 586, 697, 708
BfaI CTAG 3 cut(s) 132, 951, 968
BfmI CTRYAG 2 cut(s) 351, 408
BfrI CTTAAG 1 cut(s) 278
BfuAI ACCTGC 1 cut(s) 442
BglII AGATCT 1 cut(s) 78
BisI GCNGC 2 cut(s) 324, 327
BlpI GCTNAGC 1 cut(s) 921
BlsI GCNGC 2 cut(s) 325, 328
Bme1390I CCNGG 1 cut(s) 168
BmiI GGNNCC 2 cut(s) 113, 1005
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 495
BpiI GAAGAC 1 cut(s) 56
BplI GAGNNNNNCTC 2 cut(s) 566, 598
BpmI CTGGAG 2 cut(s) 677, 1008
Bpu10I CCTNAGC 1 cut(s) 261
Bpu1102I GCTNAGC 1 cut(s) 921
BpuEI CTTGAG 3 cut(s) 221, 520, 682
Bsa29I ATCGAT 1 cut(s) 424
BsaBI GATNNNNATC 1 cut(s) 485
BsaI GGTCTC 2 cut(s) 697, 708
BsaJI CCNNGG 1 cut(s) 65
BsaXI ACNNNNNCTCC 2 cut(s) 607, 637
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 262
Bse1I ACTGG 3 cut(s) 660, 750, 1007
Bse3DI GCAATG 2 cut(s) 861, 981
Bse8I GATNNNNATC 1 cut(s) 485
BseBI CCWGG 1 cut(s) 168
BseCI ATCGAT 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 65
BseGI GGATG 2 cut(s) 486, 575
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 2 cut(s) 121, 262
BseMI GCAATG 2 cut(s) 861, 981
BseMII CTCAG 4 cut(s) 66, 111, 275, 912
BseNI ACTGG 3 cut(s) 660, 750, 1007
BseRI GAGGAG 2 cut(s) 685, 858
BseSI GKGCMC 1 cut(s) 1006
BseXI GCAGC 2 cut(s) 335, 338
BseYI CCCAGC 1 cut(s) 152
BsgI GTGCAG 1 cut(s) 159
BshFI GGCC 1 cut(s) 255
BshNI GGYRCC 1 cut(s) 1003
BshVI ATCGAT 1 cut(s) 424
BslI CCNNNNNNNGG 2 cut(s) 121, 262
BsmAI GTCTC 4 cut(s) 546, 586, 697, 708
BsnI GGCC 1 cut(s) 255
Bso31I GGTCTC 2 cut(s) 697, 708
Bsp1286I GDGCHC 1 cut(s) 1006
Bsp143I GATC 3 cut(s) 78, 190, 506
Bsp1720I GCTNAGC 1 cut(s) 921
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 4 cut(s) 67, 112, 274, 913
BspDI ATCGAT 1 cut(s) 424
BspLI GGNNCC 2 cut(s) 113, 1005
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 1003
BspTI CTTAAG 1 cut(s) 278
BspTNI GGTCTC 2 cut(s) 697, 708
BsrDI GCAATG 2 cut(s) 861, 981
BsrI ACTGG 3 cut(s) 660, 750, 1007
BssECI CCNNGG 1 cut(s) 65
BssMI GATC 3 cut(s) 78, 190, 506
BssT1I CCWWGG 1 cut(s) 65
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 518
BstAFI CTTAAG 1 cut(s) 278
BstC8I GCNNGC 4 cut(s) 72, 253, 888, 910
BstDEI CTNAG 4 cut(s) 75, 120, 261, 921
BstF5I GGATG 2 cut(s) 486, 575
BstKTI GATC 3 cut(s) 81, 193, 509
BstMAI GTCTC 4 cut(s) 546, 586, 697, 708
BstMBI GATC 3 cut(s) 78, 190, 506
BstMWI GCNNNNNNNGC 2 cut(s) 137, 257
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 934
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 2 cut(s) 351, 408
BstSLI GKGCMC 1 cut(s) 1006
BstV1I GCAGC 2 cut(s) 335, 338
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
Bsu15I ATCGAT 1 cut(s) 424
BsuRI GGCC 1 cut(s) 255
BsuTUI ATCGAT 1 cut(s) 424
BtsCI GGATG 2 cut(s) 486, 575
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 360, 743
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 4 cut(s) 72, 253, 888, 910
ClaI ATCGAT 1 cut(s) 424
Csp6I GTAC 1 cut(s) 39
CviAII CATG 4 cut(s) 194, 605, 931, 1013
CviQI GTAC 1 cut(s) 39
DdeI CTNAG 4 cut(s) 75, 120, 261, 921
DpnI GATC 3 cut(s) 80, 192, 508
DpnII GATC 3 cut(s) 78, 190, 506
DraI TTTAAA 1 cut(s) 897
EaeI YGGCCR 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 65
Eco31I GGTCTC 2 cut(s) 697, 708
Eco57I CTGAAG 1 cut(s) 873
EcoRI GAATTC 1 cut(s) 857
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 65
ErhI CCWWGG 1 cut(s) 65
FaeI CATG 4 cut(s) 197, 608, 934, 1016
FatI CATG 4 cut(s) 193, 604, 930, 1012
FbaI TGATCA 1 cut(s) 190
FblI GTMKAC 1 cut(s) 609
Fnu4HI GCNGC 2 cut(s) 324, 327
FokI GGATG 2 cut(s) 473, 582
Fsp4HI GCNGC 2 cut(s) 324, 327
FspBI CTAG 3 cut(s) 132, 951, 968
GluI GCNGC 2 cut(s) 324, 327
GsaI CCCAGC 1 cut(s) 156
GsuI CTGGAG 2 cut(s) 677, 1008
HaeIII GGCC 1 cut(s) 255
Hin1II CATG 4 cut(s) 197, 608, 934, 1016
HincII GTYRAC 2 cut(s) 94, 610
HindII GTYRAC 2 cut(s) 94, 610
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 2 cut(s) 125, 983
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 2 cut(s) 94, 610
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 5 cut(s) 200, 311, 941, 968, 987
Hpy8I GTNNAC 2 cut(s) 94, 610
HpyAV CCTTC 6 cut(s) 317, 497, 730, 838, 895, 938
HpyCH4III ACNGT 1 cut(s) 518
HpyCH4V TGCA 3 cut(s) 140, 215, 353
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 257
HpyF3I CTNAG 4 cut(s) 75, 120, 261, 921
Hsp92II CATG 4 cut(s) 197, 608, 934, 1016
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 3 cut(s) 78, 190, 506
LmnI GCTCC 4 cut(s) 32, 111, 830, 905
Lsp1109I GCAGC 2 cut(s) 335, 338
LweI GCATC 1 cut(s) 495
MaeI CTAG 3 cut(s) 132, 951, 968
MaeIII GTNAC 2 cut(s) 790, 847
MalI GATC 3 cut(s) 80, 192, 508
MboI GATC 3 cut(s) 78, 190, 506
MboII GAAGA 3 cut(s) 61, 310, 866
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 1 cut(s) 1006
MlsI TGGCCA 1 cut(s) 255
MluCI AATT 8 cut(s) 207, 225, 341, 418, 468, 836, 857, 1022
MluNI TGGCCA 1 cut(s) 255
Mox20I TGGCCA 1 cut(s) 255
MscI TGGCCA 1 cut(s) 255
MseI TTAA 5 cut(s) 99, 279, 558, 873, 896
MslI CAYNNNNRTG 1 cut(s) 783
Msp20I TGGCCA 1 cut(s) 255
MspCI CTTAAG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 168
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 137, 257
NdeII GATC 3 cut(s) 78, 190, 506
NlaIII CATG 4 cut(s) 197, 608, 934, 1016
NlaIV GGNNCC 2 cut(s) 113, 1005
NmuCI GTSAC 2 cut(s) 790, 847
NspI RCATGY 1 cut(s) 934
PfeI GAWTC 2 cut(s) 125, 983
PfoI TCCNGGA 1 cut(s) 166
PkrI GCNGC 2 cut(s) 325, 328
PshBI ATTAAT 1 cut(s) 873
PsiI TTATAA 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 2 cut(s) 113, 1005
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 78
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
RseI CAYNNNNRTG 1 cut(s) 783
SalI GTCGAC 1 cut(s) 608
SaqAI TTAA 5 cut(s) 99, 279, 558, 873, 896
SatI GCNGC 2 cut(s) 324, 327
Sau3AI GATC 3 cut(s) 78, 190, 506
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 1006
SfaNI GCATC 1 cut(s) 495
SfcI CTRYAG 2 cut(s) 351, 408
SmiMI CAYNNNNRTG 1 cut(s) 783
SmlI CTYRAG 4 cut(s) 200, 278, 535, 697
SmoI CTYRAG 4 cut(s) 200, 278, 535, 697
Sse9I AATT 8 cut(s) 207, 225, 341, 418, 468, 836, 857, 1022
SspMI CTAG 3 cut(s) 132, 951, 968
StyD4I CCNGG 1 cut(s) 166
StyI CCWWGG 1 cut(s) 65
TaaI ACNGT 1 cut(s) 518
TaqI TCGA 3 cut(s) 424, 609, 981
TasI AATT 8 cut(s) 207, 225, 341, 418, 468, 836, 857, 1022
TfiI GAWTC 2 cut(s) 125, 983
Tru1I TTAA 5 cut(s) 99, 279, 558, 873, 896
Tru9I TTAA 5 cut(s) 99, 279, 558, 873, 896
TscAI CASTG 2 cut(s) 360, 750
TseFI GTSAC 2 cut(s) 790, 847
TseI GCWGC 2 cut(s) 323, 326
Tsp45I GTSAC 2 cut(s) 790, 847
TspDTI ATGAA 3 cut(s) 527, 850, 930
TspRI CASTG 2 cut(s) 360, 750
Vha464I CTTAAG 1 cut(s) 278
VspI ATTAAT 1 cut(s) 873
XapI RAATTY 2 cut(s) 857, 1022
XbaI TCTAGA 1 cut(s) 967
XceI RCATGY 1 cut(s) 934
XcmI CCANNNNNNNNNTGG 1 cut(s) 174
XmiI GTMKAC 1 cut(s) 609
XspI CTAG 3 cut(s) 132, 951, 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.