Rh5CG559000

Oxidoreductase family, C-terminal alpha/beta domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011989
Physical Location & Seq
Forward (+)
78264083 .. 78268794
4712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG559000.1

Sequence Viewer

Length: 1080 bp
ATGGCAGAAGCACCCCAGATTGCCATTCTTGGAGCTGGTACTTTTGTGAAGACCCAATACATTCCAAGGCTGGCTGAGATCTCTAACCTTGTCAATCTTAAAGCCATTTGGAGCCGTTCTGAGGAATCTGCCAGAGGTGCAGTTGAGATTGCTGGGAAACATTTTCCAGGAGTAGAATGCAAATGGGGTGATCATGGTCTTGAGGAAATTATTGCAGATAGTTCAATTCTTGGTGTTGCTGTGGTTTTAGCTGGCCAAGCTCAGGTTGATTTCTCACTTAAGCTTCTCAAAGCAGGGAAGAATGTCCTTCAAGAGAAACCAGCAGCAGCTACTACAAGTGAATTGGAAACTGCAGTGTCAAGTTATAAATCTATTGTTGCTAATACTCCCAATAAACCAATCTGGGCTGTAGCAGAAAATTATCGATTTGAACCTGCTTTTGTTGAGGGCAAGAAACTAATGACTGAAATTGGAGATGTGATGAGCATCCAAGTTATTGTTGAAGGATCAATGAACAGTTCAAACCCTTACTTCTCAAGCTCTTGGAGGCGAGACTTTAATGGTGGTTTCATTCTGGACATGGGAGTACATTTCATTGCAGGACTGAGGATGCTTGCTGGATGTGAGATAGTCTCGGTGTCAGCTACAACGTCTCATGTCGACAAGACTTTACCTCCCCCAGATAACATATCCTCTCTCTTTCAACTGGAGAATGGCTGTTCTGGGATTTTTGTGATGGTGGTCTCCTCAAGGTCTCCCAAGATAGTTTGGCGATTTGTTGGCTTGAAGGGAACACTGGAAGTTGAGCGTGGAAACCAAGATGGACGACACGGCTATGTGGTCACATTTTATAGTGCTGATGGACAAAGGAAAAGCTCCTTCTACCAATTTAGAGGAGTGACTGAAGAATTCATTGCTTTTATTAATGACATAAAGCAAGCCACTTTAAAGAAGGGAGCTGGCTATGAAGCTGAGCCTCGCATGTCGTTACTGGAAGGTGTTAGAGATGTTGCTGTTCTAGAGGCAATGCTCGAATCTGGAGGAAGGCAAGGGGCACCAGTCCATGTGAAGAAATTTTGA

Protein Analysis

359

Amino Acids

39.0

Weight (kDa)

6.35

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 7 - 120 1.9e-11 Oxidoreductase family, NAD-binding Rossmann fold
GFO_IDH_MocA_C3 PF22725 156 - 267 7.4e-14 GFO/IDH/MocA C-terminal domain
GFO_IDH_MocA_C PF02894 156 - 347 1.2e-11 Oxidoreductase family, C-terminal alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20790
fragaria_vesca FvH4_3g43551 FvH4_3g43551
malus_domestica MD11G1022800.v1.1
prunus_persica Prupe.6G018500_v2.0.a1
pyrus_communis pycom11g01860
rosa_chinensis RchiOBHm_Chr5g0078111
rosa_laevigata RLG00000031428 RLG00000036752
rosa_multiflora Rmu_sc0019972.1_g000004
rosa_roxburghii Rroxscaffold_1G00000370 Rroxscaffold_1G00004110
rosa_rugosa Rorug05G0458500 Rorug05G0458600
rosa_samantha Rh5AG052300 Rh5AG513200 Rh5CG559000 Rh5DG119600
rosa_wichuraiana Rw5G047600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
Acc36I ACCTGC 1 cut(s) 442
AccB1I GGYRCC 1 cut(s) 1054
AccI GTMKAC 1 cut(s) 660
AclWI GGATC 1 cut(s) 514
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 2 cut(s) 908, 1073
AcuI CTGAAG 1 cut(s) 924
AfaI GTAC 2 cut(s) 40, 588
AfiI CCNNNNNNNGG 2 cut(s) 121, 262
AflII CTTAAG 1 cut(s) 278
AgsI TTSAA 7 cut(s) 225, 311, 431, 503, 522, 704, 787
AjnI CCWGG 1 cut(s) 166
Alw26I GTCTC 5 cut(s) 546, 637, 657, 748, 759
AlwI GGATC 1 cut(s) 514
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 2 cut(s) 323, 326
ApoI RAATTY 2 cut(s) 908, 1073
AseI ATTAAT 1 cut(s) 924
AsuHPI GGTGA 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 1057
BaeI ACNNNNGTAYC 2 cut(s) 30, 63
BalI TGGCCA 1 cut(s) 255
BanI GGYRCC 1 cut(s) 1054
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
BbsI GAAGAC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 335, 338
BccI CCATC 3 cut(s) 730, 815, 854
BceAI ACGGC 2 cut(s) 99, 847
BciT130I CCWGG 1 cut(s) 168
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 5 cut(s) 546, 637, 657, 748, 759
BfaI CTAG 1 cut(s) 1019
BfmI CTRYAG 2 cut(s) 351, 408
BfrI CTTAAG 1 cut(s) 278
BfuAI ACCTGC 1 cut(s) 442
BglII AGATCT 1 cut(s) 78
BisI GCNGC 2 cut(s) 324, 327
BlpI GCTNAGC 1 cut(s) 972
BlsI GCNGC 2 cut(s) 325, 328
Bme1390I CCNGG 1 cut(s) 168
BmiI GGNNCC 2 cut(s) 113, 1056
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 2 cut(s) 495, 600
BpiI GAAGAC 1 cut(s) 56
BplI GAGNNNNNCTC 2 cut(s) 617, 649
BpmI CTGGAG 2 cut(s) 728, 1059
Bpu10I CCTNAGC 1 cut(s) 261
Bpu1102I GCTNAGC 1 cut(s) 972
BpuEI CTTGAG 3 cut(s) 221, 520, 733
Bsa29I ATCGAT 1 cut(s) 424
BsaBI GATNNNNATC 1 cut(s) 485
BsaI GGTCTC 2 cut(s) 748, 759
BsaJI CCNNGG 1 cut(s) 65
BsaXI ACNNNNNCTCC 2 cut(s) 658, 688
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 262
Bse1I ACTGG 4 cut(s) 711, 801, 996, 1058
Bse3DI GCAATG 3 cut(s) 594, 912, 1032
Bse8I GATNNNNATC 1 cut(s) 485
BseBI CCWGG 1 cut(s) 168
BseCI ATCGAT 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 65
BseGI GGATG 3 cut(s) 486, 615, 626
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 2 cut(s) 121, 262
BseMI GCAATG 3 cut(s) 594, 912, 1032
BseMII CTCAG 5 cut(s) 66, 111, 275, 596, 963
BseNI ACTGG 4 cut(s) 711, 801, 996, 1058
BseRI GAGGAG 2 cut(s) 736, 909
BseSI GKGCMC 1 cut(s) 1057
BseXI GCAGC 2 cut(s) 335, 338
BseYI CCCAGC 1 cut(s) 152
BsgI GTGCAG 1 cut(s) 159
BshFI GGCC 1 cut(s) 255
BshNI GGYRCC 1 cut(s) 1054
BshVI ATCGAT 1 cut(s) 424
BslI CCNNNNNNNGG 2 cut(s) 121, 262
BsmAI GTCTC 5 cut(s) 546, 637, 657, 748, 759
BsmBI CGTCTC 1 cut(s) 657
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 1 cut(s) 255
Bso31I GGTCTC 2 cut(s) 748, 759
Bsp1286I GDGCHC 1 cut(s) 1057
Bsp143I GATC 3 cut(s) 78, 190, 506
Bsp1720I GCTNAGC 1 cut(s) 972
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 5 cut(s) 67, 112, 274, 597, 964
BspDI ATCGAT 1 cut(s) 424
BspLI GGNNCC 2 cut(s) 113, 1056
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 1054
BspTI CTTAAG 1 cut(s) 278
BspTNI GGTCTC 2 cut(s) 748, 759
BsrDI GCAATG 3 cut(s) 594, 912, 1032
BsrI ACTGG 4 cut(s) 711, 801, 996, 1058
BssECI CCNNGG 1 cut(s) 65
BssMI GATC 3 cut(s) 78, 190, 506
BssT1I CCWWGG 1 cut(s) 65
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 518
BstAFI CTTAAG 1 cut(s) 278
BstC8I GCNNGC 5 cut(s) 72, 253, 615, 939, 961
BstDEI CTNAG 5 cut(s) 75, 120, 261, 605, 972
BstF5I GGATG 3 cut(s) 486, 615, 626
BstKTI GATC 3 cut(s) 81, 193, 509
BstMAI GTCTC 5 cut(s) 546, 637, 657, 748, 759
BstMBI GATC 3 cut(s) 78, 190, 506
BstMWI GCNNNNNNNGC 2 cut(s) 137, 257
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 985
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 2 cut(s) 351, 408
BstSLI GKGCMC 1 cut(s) 1057
BstV1I GCAGC 2 cut(s) 335, 338
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
Bsu15I ATCGAT 1 cut(s) 424
BsuRI GGCC 1 cut(s) 255
BsuTUI ATCGAT 1 cut(s) 424
BtsCI GGATG 3 cut(s) 486, 615, 626
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 360, 794
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 5 cut(s) 72, 253, 615, 939, 961
ClaI ATCGAT 1 cut(s) 424
Csp6I GTAC 2 cut(s) 39, 587
CviAII CATG 5 cut(s) 194, 580, 656, 982, 1064
CviQI GTAC 2 cut(s) 39, 587
DdeI CTNAG 5 cut(s) 75, 120, 261, 605, 972
DpnI GATC 3 cut(s) 80, 192, 508
DpnII GATC 3 cut(s) 78, 190, 506
DraI TTTAAA 1 cut(s) 948
EaeI YGGCCR 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 65
Eco31I GGTCTC 2 cut(s) 748, 759
Eco57I CTGAAG 1 cut(s) 924
EcoRI GAATTC 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 65
ErhI CCWWGG 1 cut(s) 65
Esp3I CGTCTC 1 cut(s) 657
FaeI CATG 5 cut(s) 197, 583, 659, 985, 1067
FatI CATG 5 cut(s) 193, 579, 655, 981, 1063
FbaI TGATCA 1 cut(s) 190
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 2 cut(s) 324, 327
FokI GGATG 3 cut(s) 473, 622, 633
Fsp4HI GCNGC 2 cut(s) 324, 327
FspBI CTAG 1 cut(s) 1019
GluI GCNGC 2 cut(s) 324, 327
GsaI CCCAGC 1 cut(s) 156
GsuI CTGGAG 2 cut(s) 728, 1059
HaeIII GGCC 1 cut(s) 255
Hin1II CATG 5 cut(s) 197, 583, 659, 985, 1067
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 2 cut(s) 125, 1034
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 1 cut(s) 661
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 5 cut(s) 200, 311, 575, 1019, 1038
Hpy8I GTNNAC 1 cut(s) 661
HpyAV CCTTC 7 cut(s) 317, 497, 781, 889, 946, 989, 1038
HpyCH4III ACNGT 1 cut(s) 518
HpyCH4IV ACGT 1 cut(s) 650
HpyCH4V TGCA 5 cut(s) 140, 180, 215, 353, 599
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 257
HpyF3I CTNAG 5 cut(s) 75, 120, 261, 605, 972
HpySE526I ACGT 1 cut(s) 650
Hsp92II CATG 5 cut(s) 197, 583, 659, 985, 1067
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 3 cut(s) 78, 190, 506
LmnI GCTCC 4 cut(s) 32, 111, 881, 956
Lsp1109I GCAGC 2 cut(s) 335, 338
LweI GCATC 2 cut(s) 495, 600
MaeI CTAG 1 cut(s) 1019
MaeII ACGT 1 cut(s) 650
MaeIII GTNAC 3 cut(s) 841, 898, 987
MalI GATC 3 cut(s) 80, 192, 508
MboI GATC 3 cut(s) 78, 190, 506
MboII GAAGA 3 cut(s) 61, 310, 917
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 1 cut(s) 1057
MlsI TGGCCA 1 cut(s) 255
MluCI AATT 8 cut(s) 207, 225, 341, 418, 468, 887, 908, 1073
MluNI TGGCCA 1 cut(s) 255
Mox20I TGGCCA 1 cut(s) 255
MscI TGGCCA 1 cut(s) 255
MseI TTAA 5 cut(s) 99, 279, 558, 924, 947
MslI CAYNNNNRTG 1 cut(s) 834
Msp20I TGGCCA 1 cut(s) 255
MspCI CTTAAG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 168
Mva1269I GAATGC 1 cut(s) 182
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 137, 257
NdeII GATC 3 cut(s) 78, 190, 506
NlaIII CATG 5 cut(s) 197, 583, 659, 985, 1067
NlaIV GGNNCC 2 cut(s) 113, 1056
NmuCI GTSAC 2 cut(s) 841, 898
NspI RCATGY 1 cut(s) 985
PctI GAATGC 1 cut(s) 182
PfeI GAWTC 2 cut(s) 125, 1034
PfoI TCCNGGA 1 cut(s) 166
PkrI GCNGC 2 cut(s) 325, 328
PshBI ATTAAT 1 cut(s) 924
PsiI TTATAA 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 2 cut(s) 113, 1056
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 78
RsaI GTAC 2 cut(s) 40, 588
RsaNI GTAC 2 cut(s) 39, 587
RseI CAYNNNNRTG 1 cut(s) 834
SalI GTCGAC 1 cut(s) 659
SaqAI TTAA 5 cut(s) 99, 279, 558, 924, 947
SatI GCNGC 2 cut(s) 324, 327
Sau3AI GATC 3 cut(s) 78, 190, 506
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 1057
SfaNI GCATC 2 cut(s) 495, 600
SfcI CTRYAG 2 cut(s) 351, 408
SmiMI CAYNNNNRTG 1 cut(s) 834
SmlI CTYRAG 4 cut(s) 200, 278, 535, 748
SmoI CTYRAG 4 cut(s) 200, 278, 535, 748
Sse9I AATT 8 cut(s) 207, 225, 341, 418, 468, 887, 908, 1073
SspMI CTAG 1 cut(s) 1019
StyD4I CCNGG 1 cut(s) 166
StyI CCWWGG 1 cut(s) 65
TaaI ACNGT 1 cut(s) 518
TaiI ACGT 1 cut(s) 653
TaqI TCGA 3 cut(s) 424, 660, 1032
TasI AATT 8 cut(s) 207, 225, 341, 418, 468, 887, 908, 1073
TatI WGTACW 1 cut(s) 586
TfiI GAWTC 2 cut(s) 125, 1034
Tru1I TTAA 5 cut(s) 99, 279, 558, 924, 947
Tru9I TTAA 5 cut(s) 99, 279, 558, 924, 947
TscAI CASTG 2 cut(s) 360, 801
TseFI GTSAC 2 cut(s) 841, 898
TseI GCWGC 2 cut(s) 323, 326
Tsp45I GTSAC 2 cut(s) 841, 898
TspDTI ATGAA 5 cut(s) 527, 559, 583, 901, 981
TspRI CASTG 2 cut(s) 360, 801
Vha464I CTTAAG 1 cut(s) 278
VspI ATTAAT 1 cut(s) 924
XapI RAATTY 2 cut(s) 908, 1073
XbaI TCTAGA 1 cut(s) 1018
XceI RCATGY 1 cut(s) 985
XmiI GTMKAC 1 cut(s) 660
XspI CTAG 1 cut(s) 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.