AT3G25600

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005153
Physical Location & Seq
Forward (+)
9306776 .. 9307967
1192 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25600.1

Sequence Viewer

Length: 486 bp
ATGGCGTCAACAAAACCAACCGATCAAATCAAACAACTCAAAGATATCTTCGCTCGTTTCGACATGGACAAGGACGGAAGCTTAACGCAGCTAGAACTCGCCGCTCTTCTGCGTTCTCTCGGAATCAAACCTCGCGGCGATCAAATCTCTCTTCTGTTGAACCAAATCGACCGTAACGGTAACGGATCCGTAGAGTTCGACGAGCTGGTCGTGGCGATATTGCCGGATATAAACGAAGAGGTTTTGATAAATCAAGAACAGTTGATGGAGGTTTTCCGTTCGTTTGATCGTGACGGAAACGGTTCAATAACGGCGGCGGAACTTGCTGGGTCAATGGCTAAAATGGGACATCCGTTGACTTACCGTGAATTAACGGAAATGATGACGGAAGCTGATTCTAACGGTGACGGTGTTATTAGTTTTAATGAGTTTTCTCATATTATGGCTAAATCGGCTGCTGATTTTCTTGGATTAACCGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

161

Amino Acids

17.64

Weight (kDa)

4.45

Isoelectric Point (pI)

23.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 11 - 70 1.1e-12 EF-hand domain pair
EF-hand_6 PF13405 12 - 41 2e-06 EF-hand domain
AIF-1 PF21008 79 - 150 6.4e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 86 - 148 1.6e-14 EF-hand domain pair
EF-hand_6 PF13405 87 - 115 4.7e-06 EF-hand domain
EF-hand_8 PF13833 103 - 148 2.8e-07 EF-hand domain pair
EF-hand_1 PF00036 123 - 150 8.4e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
AccBSI CCGCTC 1 cut(s) 104
AccII CGCG 1 cut(s) 135
AciI CCGC 5 cut(s) 102, 135, 314, 317, 477
AclWI GGATC 2 cut(s) 180, 193
AcyI GRCGYC 1 cut(s) 5
AgsI TTSAA 2 cut(s) 160, 306
AluBI AGCT 4 cut(s) 81, 91, 205, 392
AluI AGCT 4 cut(s) 81, 91, 205, 392
AlwI GGATC 2 cut(s) 180, 193
ApeKI GCWGC 2 cut(s) 88, 455
Asp700I GAANNNNTTC 1 cut(s) 301
AsuHPI GGTGA 1 cut(s) 416
BamHI GGATCC 1 cut(s) 185
BbvI GCAGC 2 cut(s) 100, 442
BccI CCATC 1 cut(s) 259
BceAI ACGGC 1 cut(s) 327
BfaI CTAG 1 cut(s) 92
BisI GCNGC 5 cut(s) 89, 102, 136, 315, 456
BlsI GCNGC 5 cut(s) 90, 103, 137, 316, 457
BmiI GGNNCC 1 cut(s) 187
BsaHI GRCGYC 1 cut(s) 5
BseGI GGATG 1 cut(s) 349
BseXI GCAGC 2 cut(s) 100, 442
BseYI CCCAGC 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 135
Bsh1285I CGRYCG 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 172
BsiSI CCGG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 360
BsmFI GGGAC 1 cut(s) 360
Bsp143I GATC 4 cut(s) 22, 139, 185, 286
BspACI CCGC 5 cut(s) 102, 135, 314, 317, 477
BspFNI CGCG 1 cut(s) 135
BspLI GGNNCC 1 cut(s) 187
BspPI GGATC 2 cut(s) 180, 193
BspQI GCTCTTC 1 cut(s) 111
BsrBI CCGCTC 1 cut(s) 104
BssMI GATC 4 cut(s) 22, 139, 185, 286
BssNI GRCGYC 1 cut(s) 5
Bst4CI ACNGT 7 cut(s) 173, 179, 261, 302, 365, 404, 410
Bst6I CTCTTC 3 cut(s) 111, 156, 231
BstACI GRCGYC 1 cut(s) 5
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 1 cut(s) 135
BstKTI GATC 4 cut(s) 25, 142, 188, 289
BstMBI GATC 4 cut(s) 22, 139, 185, 286
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 2 cut(s) 323, 452
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 2 cut(s) 100, 442
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BtsCI GGATG 1 cut(s) 349
CviAII CATG 1 cut(s) 64
CviJI RGCY 7 cut(s) 81, 91, 205, 338, 392, 446, 455
CviKI_1 RGCY 7 cut(s) 81, 91, 205, 338, 392, 446, 455
DpnI GATC 4 cut(s) 24, 141, 187, 288
DpnII GATC 4 cut(s) 22, 139, 185, 286
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
Eam1104I CTCTTC 3 cut(s) 111, 156, 231
EarI CTCTTC 3 cut(s) 111, 156, 231
EciI GGCGGA 1 cut(s) 332
Eco32I GATATC 1 cut(s) 46
EcoRV GATATC 1 cut(s) 46
FaeI CATG 1 cut(s) 67
FaiI YATR 4 cut(s) 65, 230, 438, 443
FaqI GGGAC 1 cut(s) 360
FatI CATG 1 cut(s) 63
Fnu4HI GCNGC 5 cut(s) 89, 102, 136, 315, 456
FokI GGATG 1 cut(s) 336
Fsp4HI GCNGC 5 cut(s) 89, 102, 136, 315, 456
FspBI CTAG 1 cut(s) 92
GluI GCNGC 5 cut(s) 89, 102, 136, 315, 456
GsaI CCCAGC 1 cut(s) 330
HapII CCGG 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 67
HincII GTYRAC 2 cut(s) 9, 357
HindII GTYRAC 2 cut(s) 9, 357
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 2 cut(s) 123, 395
HpaII CCGG 1 cut(s) 224
HphI GGTGA 1 cut(s) 416
Hpy166II GTNNAC 2 cut(s) 9, 357
Hpy188I TCNGA 1 cut(s) 122
Hpy188III TCNNGA 3 cut(s) 254, 290, 483
Hpy8I GTNNAC 2 cut(s) 9, 357
Hpy99I CGWCG 1 cut(s) 203
HpyCH4III ACNGT 7 cut(s) 173, 179, 261, 302, 365, 404, 410
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 452
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 67
Kzo9I GATC 4 cut(s) 22, 139, 185, 286
LguI GCTCTTC 1 cut(s) 111
LpnPI CCDG 3 cut(s) 191, 237, 312
Lsp1109I GCAGC 2 cut(s) 100, 442
MaeI CTAG 1 cut(s) 92
MaeIII GTNAC 4 cut(s) 173, 179, 290, 404
MalI GATC 4 cut(s) 24, 141, 187, 288
MbiI CCGCTC 1 cut(s) 104
MboI GATC 4 cut(s) 22, 139, 185, 286
MboII GAAGA 4 cut(s) 40, 98, 143, 248
MflI RGATCY 1 cut(s) 185
MluCI AATT 1 cut(s) 368
MnlI CCTC 3 cut(s) 141, 232, 262
MroXI GAANNNNTTC 1 cut(s) 301
MseI TTAA 4 cut(s) 83, 371, 423, 473
MspI CCGG 1 cut(s) 224
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 2 cut(s) 323, 452
NdeII GATC 4 cut(s) 22, 139, 185, 286
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 2 cut(s) 290, 404
PciSI GCTCTTC 1 cut(s) 111
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 207
PdmI GAANNNNTTC 1 cut(s) 301
PfeI GAWTC 2 cut(s) 123, 395
PkrI GCNGC 5 cut(s) 90, 103, 137, 316, 457
PspFI CCCAGC 1 cut(s) 326
PspN4I GGNNCC 1 cut(s) 187
PsuI RGATCY 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 4 cut(s) 83, 371, 423, 473
SatI GCNGC 5 cut(s) 89, 102, 136, 315, 456
Sau3AI GATC 4 cut(s) 22, 139, 185, 286
SetI ASST 7 cut(s) 83, 93, 133, 207, 243, 273, 394
Sse9I AATT 1 cut(s) 368
SsiI CCGC 5 cut(s) 102, 135, 314, 317, 477
SspMI CTAG 1 cut(s) 92
TaaI ACNGT 7 cut(s) 173, 179, 261, 302, 365, 404, 410
TaqI TCGA 3 cut(s) 60, 168, 198
TasI AATT 1 cut(s) 368
TauI GCSGC 3 cut(s) 104, 138, 317
TfiI GAWTC 2 cut(s) 123, 395
Tru1I TTAA 4 cut(s) 83, 371, 423, 473
Tru9I TTAA 4 cut(s) 83, 371, 423, 473
TseFI GTSAC 2 cut(s) 290, 404
TseI GCWGC 2 cut(s) 88, 455
Tsp45I GTSAC 2 cut(s) 290, 404
TspGWI ACGGA 8 cut(s) 90, 178, 198, 266, 309, 342, 389, 401
XmnI GAANNNNTTC 1 cut(s) 301
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.