MD13G1050700.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005153
Physical Location & Seq
Reverse (-)
3620676 .. 3621152
477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1050700.v1.1.491

Sequence Viewer

Length: 477 bp
ATGATGGCCGCGCTCGGATCCGATCAACTGAAGCAGCTGAAGGACATCTTCATGCGATTCGACATGGACTCCGACGGCAGCCTCACCCAGCTCGAACTCGCCGCCCTCCTCCGCTCCCTCGGCCTCAAGCCCACCGGCGACCAGCTCCACGTTTTCCTCTCCAACATCGACGCCAACGGCAACGGTGCCATCGAGTTCGACGAGCTCGTCACCGCCATCCTCCCAGACATGAACGAGGAGATTTTGATCAACCAGGAGCAGCTCATGGAGGTCTTCCGGTCGTTCGACCGCGACGGCAACGGCTACATCACAGCCGCCGAGCTCGCCGGATCCATGGCCAAAATGGGCCACCCCTTGACGTACCGGGAGCTCTCCGATATGATGAGGGAGGCCGACACGAACGGCGACGGCGTCATTAGCTTCAACGAATTCGCGACCATCATGTCTCGGTCCGCCACTGATTTTCTCGGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

159

Amino Acids

17.41

Weight (kDa)

4.23

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 11 - 73 3.1e-11 EF-hand domain pair
EF-hand_6 PF13405 12 - 41 9e-06 EF-hand domain
EF-hand_7 PF13499 86 - 149 2.7e-16 EF-hand domain pair
EF-hand_6 PF13405 87 - 115 9.5e-07 EF-hand domain
EF-hand_1 PF00036 88 - 114 3.1e-06 EF hand domain
EF-hand_8 PF13833 112 - 148 5.9e-08 EF-hand domain pair
EF-hand_1 PF00036 123 - 150 1.3e-08 EF hand domain
EF-hand_5 PF13202 126 - 148 6.5e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 206, 442
AccB1I GGYRCC 1 cut(s) 185
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 3 cut(s) 11, 291, 434
AciI CCGC 7 cut(s) 9, 102, 112, 213, 289, 315, 453
AclWI GGATC 4 cut(s) 12, 25, 324, 337
AcoI YGGCCR 2 cut(s) 6, 336
AcsI RAATTY 1 cut(s) 428
AcuI CTGAAG 2 cut(s) 50, 59
AcyI GRCGYC 2 cut(s) 171, 411
AfaI GTAC 1 cut(s) 362
AgsI TTSAA 1 cut(s) 424
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 8 cut(s) 37, 91, 145, 205, 262, 322, 370, 420
AluI AGCT 8 cut(s) 37, 91, 145, 205, 262, 322, 370, 420
Alw21I GWGCWC 3 cut(s) 207, 324, 372
Alw26I GTCTC 1 cut(s) 450
AlwI GGATC 4 cut(s) 12, 25, 324, 337
Ama87I CYCGRG 1 cut(s) 467
AoxI GGCC 5 cut(s) 6, 121, 336, 346, 390
ApeKI GCWGC 3 cut(s) 34, 78, 259
ApoI RAATTY 1 cut(s) 428
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 2 cut(s) 346, 450
AsuC2I CCSGG 1 cut(s) 365
AsuHPI GGTGA 2 cut(s) 76, 202
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 1 cut(s) 450
BalI TGGCCA 1 cut(s) 338
BamHI GGATCC 2 cut(s) 17, 329
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 3 cut(s) 207, 324, 372
BbsI GAAGAC 1 cut(s) 265
Bbv12I GWGCWC 3 cut(s) 207, 324, 372
BbvI GCAGC 3 cut(s) 46, 90, 271
BccI CCATC 3 cut(s) 197, 224, 446
BceAI ACGGC 6 cut(s) 91, 193, 310, 316, 418, 424
BciT130I CCWGG 1 cut(s) 254
BclI TGATCA 1 cut(s) 246
BcnI CCSGG 1 cut(s) 365
BcoDI GTCTC 1 cut(s) 450
BisI GCNGC 6 cut(s) 9, 35, 79, 102, 260, 315
BlsI GCNGC 6 cut(s) 10, 36, 80, 103, 261, 316
Bme1390I CCNGG 2 cut(s) 254, 365
Bme18I GGWCC 1 cut(s) 450
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 2 cut(s) 346, 450
BmiI GGNNCC 3 cut(s) 19, 187, 331
BmrFI CCNGG 2 cut(s) 254, 365
BpiI GAAGAC 1 cut(s) 265
BpuEI CTTGAG 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 365
BsaBI GATNNNNATC 1 cut(s) 245
BsaHI GRCGYC 2 cut(s) 171, 411
BsaJI CCNNGG 2 cut(s) 118, 333
BsaWI WCCGGW 1 cut(s) 276
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bse118I RCCGGY 1 cut(s) 134
Bse8I GATNNNNATC 1 cut(s) 245
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 2 cut(s) 118, 333
BseGI GGATG 1 cut(s) 216
BseJI GATNNNNATC 1 cut(s) 245
BseRI GAGGAG 2 cut(s) 98, 251
BseXI GCAGC 3 cut(s) 46, 90, 271
BseYI CCCAGC 1 cut(s) 87
Bsh1236I CGCG 3 cut(s) 11, 291, 434
Bsh1285I CGRYCG 2 cut(s) 281, 289
BshFI GGCC 5 cut(s) 8, 123, 338, 348, 392
BshNI GGYRCC 1 cut(s) 185
BsiEI CGRYCG 2 cut(s) 281, 289
BsiHKAI GWGCWC 3 cut(s) 207, 324, 372
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 4 cut(s) 135, 277, 327, 364
BsmAI GTCTC 1 cut(s) 450
BsnI GGCC 5 cut(s) 8, 123, 338, 348, 392
BsoBI CYCGRG 1 cut(s) 467
Bsp1286I GDGCHC 3 cut(s) 207, 324, 372
Bsp143I GATC 4 cut(s) 17, 22, 246, 329
Bsp19I CCATGG 1 cut(s) 333
Bsp68I TCGCGA 1 cut(s) 434
BspACI CCGC 7 cut(s) 9, 102, 112, 213, 289, 315, 453
BspANI GGCC 5 cut(s) 8, 123, 338, 348, 392
BspFNI CGCG 3 cut(s) 11, 291, 434
BspLI GGNNCC 3 cut(s) 19, 187, 331
BspPI GGATC 4 cut(s) 12, 25, 324, 337
BspT107I GGYRCC 1 cut(s) 185
BsrBI CCGCTC 1 cut(s) 114
BsrFI RCCGGY 1 cut(s) 134
BssAI RCCGGY 1 cut(s) 134
BssECI CCNNGG 2 cut(s) 118, 333
BssMI GATC 4 cut(s) 17, 22, 246, 329
BssNI GRCGYC 2 cut(s) 171, 411
BssT1I CCWWGG 1 cut(s) 333
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 185
BstACI GRCGYC 2 cut(s) 171, 411
BstC8I GCNNGC 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 216
BstFNI CGCG 3 cut(s) 11, 291, 434
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 4 cut(s) 20, 25, 249, 332
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 4 cut(s) 17, 22, 246, 329
BstMCI CGRYCG 2 cut(s) 281, 289
BstMWI GCNNNNNNNGC 3 cut(s) 120, 323, 417
BstNI CCWGG 1 cut(s) 254
BstSCI CCNGG 2 cut(s) 252, 363
BstUI CGCG 3 cut(s) 11, 291, 434
BstV1I GCAGC 3 cut(s) 46, 90, 271
BstV2I GAAGAC 1 cut(s) 265
BstX2I RGATCY 2 cut(s) 17, 329
BstYI RGATCY 2 cut(s) 17, 329
BsuRI GGCC 5 cut(s) 8, 123, 338, 348, 392
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 456
BtuMI TCGCGA 1 cut(s) 434
Cac8I GCNNGC 1 cut(s) 324
CfoI GCGC 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 134
Cfr13I GGNCC 2 cut(s) 346, 450
CpoI CGGWCCG 1 cut(s) 450
CseI GACGC 2 cut(s) 179, 400
Csp6I GTAC 1 cut(s) 361
CspI CGGWCCG 1 cut(s) 450
CviAII CATG 6 cut(s) 52, 64, 229, 265, 334, 442
CviQI GTAC 1 cut(s) 361
DpnI GATC 4 cut(s) 19, 24, 248, 331
DpnII GATC 4 cut(s) 17, 22, 246, 329
DrdI GACNNNNNNGTC 2 cut(s) 206, 442
DseDI GACNNNNNNGTC 2 cut(s) 206, 442
EaeI YGGCCR 2 cut(s) 6, 336
EciI GGCGGA 1 cut(s) 442
Ecl136II GAGCTC 3 cut(s) 205, 322, 370
Eco130I CCWWGG 1 cut(s) 333
Eco24I GRGCYC 3 cut(s) 207, 324, 372
Eco47I GGWCC 1 cut(s) 450
Eco53kI GAGCTC 3 cut(s) 205, 322, 370
Eco57I CTGAAG 2 cut(s) 50, 59
Eco88I CYCGRG 1 cut(s) 467
EcoICRI GAGCTC 3 cut(s) 205, 322, 370
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 333
EcoT38I GRGCYC 3 cut(s) 207, 324, 372
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 6 cut(s) 55, 67, 232, 268, 337, 445
FaiI YATR 7 cut(s) 53, 65, 230, 266, 335, 380, 443
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FatI CATG 6 cut(s) 51, 63, 228, 264, 333, 441
FbaI TGATCA 1 cut(s) 246
Fnu4HI GCNGC 6 cut(s) 9, 35, 79, 102, 260, 315
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 3 cut(s) 207, 324, 372
Fsp4HI GCNGC 6 cut(s) 9, 35, 79, 102, 260, 315
GlaI GCGC 1 cut(s) 12
GluI GCNGC 6 cut(s) 9, 35, 79, 102, 260, 315
GsaI CCCAGC 1 cut(s) 91
HaeIII GGCC 5 cut(s) 8, 123, 338, 348, 392
HapII CCGG 4 cut(s) 135, 277, 327, 364
HgaI GACGC 2 cut(s) 179, 400
HhaI GCGC 1 cut(s) 13
Hin1I GRCGYC 2 cut(s) 171, 411
Hin1II CATG 6 cut(s) 55, 67, 232, 268, 337, 445
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HinfI GANTC 2 cut(s) 57, 68
HpaII CCGG 4 cut(s) 135, 277, 327, 364
HphI GGTGA 2 cut(s) 76, 202
Hpy188I TCNGA 4 cut(s) 17, 22, 73, 376
Hpy188III TCNNGA 2 cut(s) 433, 469
Hpy99I CGWCG 5 cut(s) 77, 173, 203, 296, 410
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 1 cut(s) 185
HpyCH4IV ACGT 2 cut(s) 150, 359
HpyF10VI GCNNNNNNNGC 3 cut(s) 120, 323, 417
HpySE526I ACGT 2 cut(s) 150, 359
Hsp92I GRCGYC 2 cut(s) 171, 411
Hsp92II CATG 6 cut(s) 55, 67, 232, 268, 337, 445
HspAI GCGC 1 cut(s) 11
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 4 cut(s) 17, 22, 246, 329
LmnI GCTCC 4 cut(s) 119, 150, 256, 367
LpnPI CCDG 9 cut(s) 101, 148, 155, 237, 239, 266, 290, 340, 377
Lsp1109I GCAGC 3 cut(s) 46, 90, 271
MaeII ACGT 2 cut(s) 150, 359
MaeIII GTNAC 1 cut(s) 208
MalI GATC 4 cut(s) 19, 24, 248, 331
MbiI CCGCTC 1 cut(s) 114
MboI GATC 4 cut(s) 17, 22, 246, 329
MboII GAAGA 2 cut(s) 40, 265
MflI RGATCY 2 cut(s) 17, 329
MhlI GDGCHC 3 cut(s) 207, 324, 372
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 338
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 2 cut(s) 96, 186
Mox20I TGGCCA 1 cut(s) 338
MscI TGGCCA 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 4 cut(s) 135, 277, 327, 364
MspR9I CCNGG 2 cut(s) 254, 365
MvaI CCWGG 1 cut(s) 254
MvnI CGCG 3 cut(s) 11, 291, 434
MwoI GCNNNNNNNGC 3 cut(s) 120, 323, 417
NciI CCSGG 1 cut(s) 365
NcoI CCATGG 1 cut(s) 333
NdeII GATC 4 cut(s) 17, 22, 246, 329
NlaIII CATG 6 cut(s) 55, 67, 232, 268, 337, 445
NlaIV GGNNCC 3 cut(s) 19, 187, 331
NmeAIII GCCGAG 2 cut(s) 99, 343
NmuCI GTSAC 1 cut(s) 208
NruI TCGCGA 1 cut(s) 434
PcsI WCGNNNNNNNCGW 4 cut(s) 189, 198, 204, 408
PfeI GAWTC 1 cut(s) 57
PflFI GACNNNGTC 1 cut(s) 410
PkrI GCNGC 6 cut(s) 10, 36, 80, 103, 261, 316
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Psp124BI GAGCTC 3 cut(s) 207, 324, 372
Psp6I CCWGG 1 cut(s) 252
PspFI CCCAGC 1 cut(s) 87
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 3 cut(s) 19, 187, 331
PspPI GGNCC 2 cut(s) 346, 450
PsuI RGATCY 2 cut(s) 17, 329
PsyI GACNNNGTC 1 cut(s) 410
PvuII CAGCTG 1 cut(s) 37
RruI TCGCGA 1 cut(s) 434
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 1 cut(s) 50
Rsr2I CGGWCCG 1 cut(s) 450
RsrII CGGWCCG 1 cut(s) 450
SacI GAGCTC 3 cut(s) 207, 324, 372
SatI GCNGC 6 cut(s) 9, 35, 79, 102, 260, 315
Sau3AI GATC 4 cut(s) 17, 22, 246, 329
Sau96I GGNCC 2 cut(s) 346, 450
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 254, 365
SduI GDGCHC 3 cut(s) 207, 324, 372
SgrAI CRCCGGYG 1 cut(s) 134
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 1 cut(s) 428
SsiI CCGC 7 cut(s) 9, 102, 112, 213, 289, 315, 453
SstI GAGCTC 3 cut(s) 207, 324, 372
StyD4I CCNGG 2 cut(s) 252, 363
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 1 cut(s) 185
TaiI ACGT 2 cut(s) 153, 362
TaqI TCGA 6 cut(s) 60, 93, 168, 192, 198, 285
TaqII GACCGA 1 cut(s) 438
TasI AATT 1 cut(s) 428
TauI GCSGC 3 cut(s) 11, 104, 317
TfiI GAWTC 1 cut(s) 57
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 3 cut(s) 34, 78, 259
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 40, 245
TspRI CASTG 1 cut(s) 463
Tth111I GACNNNGTC 1 cut(s) 410
VpaK11BI GGWCC 1 cut(s) 450
XapI RAATTY 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.