Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005153

Physical Location & Seq

Genomic Coordinates Forward (+)

30192335 .. 30192817

Gene Structure 483 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1219400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 483 bp

ATGCCTGCACTACGAGTTGATCAAATCAACCAGCTAAGAGACATATTTGCAAGATTCGACATGGACTCCGACGGCAGCCTCACCATCTTGGAGCTCACCACCCTCCTCCGCTCGCTCGGCCTCAAGTCCTCCGGTGACCAAGTCCACGTCCTCCTAGCCAACATGGATGCAAACGGCAATGGCTCCGTGGAATTCGACGGGCTGGTCAGTGCCATATTGCCCGACATGGACGAGGAGATTATGGTAAACCAAAAGCAACTCTTGGAGGTTTTTCGCTCTTTTGAACATGACGGCAACGGTTACATTACTGCGGCAGAGCTGGCATGGTCCATGGCGAAAATGGGGCAACCGTTGACGTATAAGGAGGTGACGGAGACGATCAAAGAGGCTGATACGGATGGAGATGGTGTCATTAGTTTCAATGAATTTGCTACAATAATGGCGAAATCGGCGTCGAGTTTTCTAGGCCTTGCTGCCTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

161

Amino Acids

17.41

Weight (kDa)

4.27

Isoelectric Point (pI)

31.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	10 - 71	3.4e-10	EF-hand domain pair
EF-hand_7	PF13499	85 - 147	2.3e-15	EF-hand domain pair
EF-hand_8	PF13833	99 - 147	8.2e-10	EF-hand domain pair
EF-hand_1	PF00036	122 - 149	9.1e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005153)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25600
fragaria_vesca	FvH4_4g28451
malus_domestica	MD06G1209000.v1.1 MD13G1050700.v1.1 MD14G1219400.v1.1 MD16G1051200.v1.1
prunus_persica	Prupe.1G301400_v2.0.a1 Prupe.5G214100_v2.0.a1
pyrus_communis	pycom13g04480 pycom14g18180 pycom16g04500
rosa_chinensis	RchiOBHm_Chr4g0436421 RchiOBHm_Chr7g0184291
rosa_laevigata	RLG00000004989 RLG00000006520
rosa_multiflora	Rmu_co8043624.1_g000001 Rmu_co8189102.1_g000001 Rmu_sc0013812.1_g000006
rosa_roxburghii	Rroxscaffold_3G00269760 Rroxscaffold_5G00377580
rosa_rugosa	Rorug04G0293700
rosa_samantha	Rh4AG348200 Rh4BG356800 Rh4DG350600 Rh7AG081400 Rh7BG065900 Rh7CG066200 Rh7DG066000
rosa_wichuraiana	Rw4G030430 Rw7G005400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	203
AccBSI	CCGCTC	1 cut(s)	111
AciI	CCGC	2 cut(s)	109, 311
AcsI	RAATTY	2 cut(s)	191, 425
AcyI	GRCGYC	1 cut(s)	452
AgsI	TTSAA	2 cut(s)	284, 421
AjiI	CACGTC	1 cut(s)	148
AluBI	AGCT	3 cut(s)	34, 94, 319
AluI	AGCT	3 cut(s)	34, 94, 319
Alw21I	GWGCWC	1 cut(s)	96
Alw26I	GTCTC	2 cut(s)	33, 368
AoxI	GGCC	2 cut(s)	118, 466
ApeKI	GCWGC	2 cut(s)	75, 473
ApoI	RAATTY	2 cut(s)	191, 425
ArsI	GACNNNNNNTTYG	2 cut(s)	437, 469
AspS9I	GGNCC	1 cut(s)	327
AsuHPI	GGTGA	4 cut(s)	73, 88, 146, 379
AvaII	GGWCC	1 cut(s)	327
BanII	GRGCYC	1 cut(s)	96
Bbv12I	GWGCWC	1 cut(s)	96
BbvI	GCAGC	2 cut(s)	87, 460
BccI	CCATC	3 cut(s)	92, 392, 398
BceAI	ACGGC	3 cut(s)	88, 190, 307
BclI	TGATCA	1 cut(s)	19
BcoDI	GTCTC	2 cut(s)	33, 368
BfaI	CTAG	2 cut(s)	155, 464
BisI	GCNGC	3 cut(s)	76, 312, 474
BlsI	GCNGC	3 cut(s)	77, 313, 475
Bme18I	GGWCC	1 cut(s)	327
BmgBI	CACGTC	1 cut(s)	148
BmgT120I	GGNCC	1 cut(s)	327
BmiI	GGNNCC	1 cut(s)	184
BmsI	GCATC	1 cut(s)	157
BpuEI	CTTGAG	1 cut(s)	107
BsaHI	GRCGYC	1 cut(s)	452
BsaJI	CCNNGG	2 cut(s)	186, 330
BsaWI	WCCGGW	1 cut(s)	131
BsaXI	ACNNNNNCTCC	4 cut(s)	50, 80, 393, 423
Bse3DI	GCAATG	1 cut(s)	184
BseDI	CCNNGG	2 cut(s)	186, 330
BseGI	GGATG	2 cut(s)	172, 403
BseMI	GCAATG	1 cut(s)	184
BseRI	GAGGAG	2 cut(s)	95, 248
BseXI	GCAGC	2 cut(s)	87, 460
BshFI	GGCC	2 cut(s)	120, 468
BsiHKAI	GWGCWC	1 cut(s)	96
BsiSI	CCGG	1 cut(s)	132
BsmAI	GTCTC	2 cut(s)	33, 368
BsmBI	CGTCTC	1 cut(s)	368
BsnI	GGCC	2 cut(s)	120, 468
Bsp1286I	GDGCHC	1 cut(s)	96
Bsp143I	GATC	2 cut(s)	19, 378
Bsp19I	CCATGG	1 cut(s)	330
BspACI	CCGC	2 cut(s)	109, 311
BspANI	GGCC	2 cut(s)	120, 468
BspHI	TCATGA	1 cut(s)	479
BspLI	GGNNCC	1 cut(s)	184
BsrBI	CCGCTC	1 cut(s)	111
BsrDI	GCAATG	1 cut(s)	184
BssECI	CCNNGG	2 cut(s)	186, 330
BssMI	GATC	2 cut(s)	19, 378
BssNI	GRCGYC	1 cut(s)	452
BssT1I	CCWWGG	1 cut(s)	330
Bst4CI	ACNGT	2 cut(s)	299, 351
BstACI	GRCGYC	1 cut(s)	452
BstC8I	GCNNGC	3 cut(s)	6, 113, 321
BstDEI	CTNAG	1 cut(s)	35
BstDSI	CCRYGG	2 cut(s)	186, 330
BstEII	GGTNACC	1 cut(s)	134
BstF5I	GGATG	2 cut(s)	172, 403
BstKTI	GATC	2 cut(s)	22, 381
BstMAI	GTCTC	2 cut(s)	33, 368
BstMBI	GATC	2 cut(s)	19, 378
BstMWI	GCNNNNNNNGC	3 cut(s)	117, 320, 449
BstPI	GGTNACC	1 cut(s)	134
BstV1I	GCAGC	2 cut(s)	87, 460
BsuRI	GGCC	2 cut(s)	120, 468
BtgI	CCRYGG	2 cut(s)	186, 330
BtrI	CACGTC	1 cut(s)	148
BtsCI	GGATG	2 cut(s)	172, 403
BtsIMutI	CAGTG	1 cut(s)	214
Cac8I	GCNNGC	3 cut(s)	6, 113, 321
CciI	TCATGA	1 cut(s)	479
Cfr13I	GGNCC	1 cut(s)	327
CseI	GACGC	1 cut(s)	441
CviAII	CATG	7 cut(s)	61, 163, 226, 287, 324, 331, 480
DdeI	CTNAG	1 cut(s)	35
DpnI	GATC	2 cut(s)	21, 380
DpnII	GATC	2 cut(s)	19, 378
DrdI	GACNNNNNNGTC	1 cut(s)	203
DseDI	GACNNNNNNGTC	1 cut(s)	203
Ecl136II	GAGCTC	1 cut(s)	94
Eco130I	CCWWGG	1 cut(s)	330
Eco147I	AGGCCT	1 cut(s)	468
Eco24I	GRGCYC	1 cut(s)	96
Eco47I	GGWCC	1 cut(s)	327
Eco53kI	GAGCTC	1 cut(s)	94
Eco91I	GGTNACC	1 cut(s)	134
EcoICRI	GAGCTC	1 cut(s)	94
EcoO65I	GGTNACC	1 cut(s)	134
EcoRI	GAATTC	1 cut(s)	191
EcoT14I	CCWWGG	1 cut(s)	330
EcoT38I	GRGCYC	1 cut(s)	96
ErhI	CCWWGG	1 cut(s)	330
Esp3I	CGTCTC	1 cut(s)	368
FaeI	CATG	7 cut(s)	64, 166, 229, 290, 327, 334, 483
FalI	AAGNNNNNCTT	2 cut(s)	245, 277
FatI	CATG	7 cut(s)	60, 162, 225, 286, 323, 330, 479
FbaI	TGATCA	1 cut(s)	19
Fnu4HI	GCNGC	3 cut(s)	76, 312, 474
FokI	GGATG	2 cut(s)	179, 410
FriOI	GRGCYC	1 cut(s)	96
Fsp4HI	GCNGC	3 cut(s)	76, 312, 474
FspBI	CTAG	2 cut(s)	155, 464
GluI	GCNGC	3 cut(s)	76, 312, 474
HaeIII	GGCC	2 cut(s)	120, 468
HapII	CCGG	1 cut(s)	132
HgaI	GACGC	1 cut(s)	441
Hin1I	GRCGYC	1 cut(s)	452
Hin1II	CATG	7 cut(s)	64, 166, 229, 290, 327, 334, 483
HincII	GTYRAC	1 cut(s)	354
HindII	GTYRAC	1 cut(s)	354
HinfI	GANTC	2 cut(s)	54, 65
HpaII	CCGG	1 cut(s)	132
HphI	GGTGA	4 cut(s)	73, 88, 146, 379
Hpy166II	GTNNAC	3 cut(s)	145, 247, 354
Hpy188I	TCNGA	1 cut(s)	70
Hpy188III	TCNNGA	1 cut(s)	480
Hpy8I	GTNNAC	3 cut(s)	145, 247, 354
Hpy99I	CGWCG	3 cut(s)	74, 200, 457
HpyCH4III	ACNGT	2 cut(s)	299, 351
HpyCH4IV	ACGT	2 cut(s)	147, 356
HpyCH4V	TGCA	3 cut(s)	8, 50, 170
HpyF10VI	GCNNNNNNNGC	3 cut(s)	117, 320, 449
HpyF3I	CTNAG	1 cut(s)	35
HpySE526I	ACGT	2 cut(s)	147, 356
Hsp92I	GRCGYC	1 cut(s)	452
Hsp92II	CATG	7 cut(s)	64, 166, 229, 290, 327, 334, 483
Ksp22I	TGATCA	1 cut(s)	19
Kzo9I	GATC	2 cut(s)	19, 378
LmnI	GCTCC	2 cut(s)	91, 188
LpnPI	CCDG	5 cut(s)	18, 44, 145, 188, 305
Lsp1109I	GCAGC	2 cut(s)	87, 460
LweI	GCATC	1 cut(s)	157
MaeI	CTAG	2 cut(s)	155, 464
MaeII	ACGT	2 cut(s)	147, 356
MaeIII	GTNAC	3 cut(s)	134, 299, 367
MalI	GATC	2 cut(s)	21, 380
MbiI	CCGCTC	1 cut(s)	111
MboI	GATC	2 cut(s)	19, 378
MhlI	GDGCHC	1 cut(s)	96
MluCI	AATT	2 cut(s)	191, 425
MlyI	GAGTC	1 cut(s)	59
MmeI	TCCRAC	1 cut(s)	93
MspI	CCGG	1 cut(s)	132
MwoI	GCNNNNNNNGC	3 cut(s)	117, 320, 449
NcoI	CCATGG	1 cut(s)	330
NdeII	GATC	2 cut(s)	19, 378
NlaIII	CATG	7 cut(s)	64, 166, 229, 290, 327, 334, 483
NlaIV	GGNNCC	1 cut(s)	184
NmeAIII	GCCGAG	1 cut(s)	96
NmuCI	GTSAC	2 cut(s)	134, 367
PagI	TCATGA	1 cut(s)	479
PceI	AGGCCT	1 cut(s)	468
PfeI	GAWTC	1 cut(s)	54
PflFI	GACNNNGTC	1 cut(s)	140
PkrI	GCNGC	3 cut(s)	77, 313, 475
PleI	GAGTC	1 cut(s)	59
PpsI	GAGTC	1 cut(s)	59
Psp124BI	GAGCTC	1 cut(s)	96
PspEI	GGTNACC	1 cut(s)	134
PspN4I	GGNNCC	1 cut(s)	184
PspPI	GGNCC	1 cut(s)	327
PsyI	GACNNNGTC	1 cut(s)	140
SacI	GAGCTC	1 cut(s)	96
SatI	GCNGC	3 cut(s)	76, 312, 474
Sau3AI	GATC	2 cut(s)	19, 378
Sau96I	GGNCC	1 cut(s)	327
SchI	GAGTC	1 cut(s)	59
SduI	GDGCHC	1 cut(s)	96
SetI	ASST	7 cut(s)	36, 96, 150, 270, 321, 359, 369
SfaNI	GCATC	1 cut(s)	157
SinI	GGWCC	1 cut(s)	327
SmlI	CTYRAG	1 cut(s)	122
SmoI	CTYRAG	1 cut(s)	122
Sse9I	AATT	2 cut(s)	191, 425
SseBI	AGGCCT	1 cut(s)	468
SsiI	CCGC	2 cut(s)	109, 311
SspMI	CTAG	2 cut(s)	155, 464
SstI	GAGCTC	1 cut(s)	96
StuI	AGGCCT	1 cut(s)	468
StyI	CCWWGG	1 cut(s)	330
TaaI	ACNGT	2 cut(s)	299, 351
TaiI	ACGT	2 cut(s)	150, 359
TaqI	TCGA	3 cut(s)	57, 195, 455
TasI	AATT	2 cut(s)	191, 425
TauI	GCSGC	1 cut(s)	314
TfiI	GAWTC	1 cut(s)	54
TscAI	CASTG	1 cut(s)	214
TseFI	GTSAC	2 cut(s)	134, 367
TseI	GCWGC	2 cut(s)	75, 473
Tsp45I	GTSAC	2 cut(s)	134, 367
TspDTI	ATGAA	1 cut(s)	438
TspGWI	ACGGA	3 cut(s)	175, 386, 410
TspRI	CASTG	1 cut(s)	214
Tth111I	GACNNNGTC	1 cut(s)	140
VpaK11BI	GGWCC	1 cut(s)	327
XapI	RAATTY	2 cut(s)	191, 425
XcmI	CCANNNNNNNNNTGG	1 cut(s)	337
XspI	CTAG	2 cut(s)	155, 464

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.