RLG00000006520

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005153
Physical Location & Seq
Forward (+)
7992074 .. 7992893
820 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006520

Sequence Viewer

Length: 462 bp
ATGGCTAGGCTCGAGACAGACCATCTCAAACAGCTTAAAGACATCTTCATGCGGTTCGACATGGACTCCGATGGTAGCCTAACCCACCTGGAGCTGGCCGCCCTGCTCCGCTCCCTAGGGGTCAAGCCCCAGGGCGACCAGCTCCACAAGTTAATGGCCAACATGGACGCCAATGGAAACGGCGCCGTTGAGTTCGAGGAGTTGGTAAACGCCATCATGCCTAACATGAACGACGAGATTCTTGTCAACCAAGAGCAGCTCATGGAGGTGTTCCGGTCGTTCGACCGCGACGGTAACGGCTACATAACGGCGGCAGAGCTGGCAGGGTCGATGGCCAAGATGGGCCACCCCTTGACGTACAGGGAGTTGTCGGACATGATGAGGGAGGCGGACACCAACGGTGACGGTGTCATCAGCTTCAACGAGTTCGCCACCATCATGTCTCGATTTACTTTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

154

Amino Acids

17.2

Weight (kDa)

4.58

Isoelectric Point (pI)

29.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 10 - 72 1.6e-11 EF-hand domain pair
EF-hand_7 PF13499 85 - 148 2.5e-16 EF-hand domain pair
EF-hand_6 PF13405 86 - 114 9e-07 EF-hand domain
EF-hand_1 PF00036 87 - 113 3e-06 EF hand domain
EF-hand_8 PF13833 102 - 147 1.8e-08 EF-hand domain pair
EF-hand_1 PF00036 122 - 149 9.6e-09 EF hand domain
EF-hand_5 PF13202 125 - 147 6.2e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 182
AccBSI CCGCTC 1 cut(s) 111
AccII CGCG 1 cut(s) 288
AciI CCGC 6 cut(s) 52, 99, 109, 286, 311, 389
AcoI YGGCCR 3 cut(s) 96, 156, 333
AcyI GRCGYC 2 cut(s) 168, 183
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 1 cut(s) 421
AjnI CCWGG 2 cut(s) 87, 129
AluBI AGCT 6 cut(s) 34, 94, 142, 259, 319, 417
AluI AGCT 6 cut(s) 34, 94, 142, 259, 319, 417
Alw26I GTCTC 2 cut(s) 8, 447
Ama87I CYCGRG 1 cut(s) 11
AoxI GGCC 4 cut(s) 96, 156, 333, 343
ApeKI GCWGC 1 cut(s) 256
AspA2I CCTAGG 1 cut(s) 115
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 343
AsuHPI GGTGA 1 cut(s) 413
AvaI CYCGRG 1 cut(s) 11
AvrII CCTAGG 1 cut(s) 115
BalI TGGCCA 2 cut(s) 158, 335
BanI GGYRCC 1 cut(s) 182
BbvI GCAGC 1 cut(s) 268
BccI CCATC 6 cut(s) 30, 65, 221, 325, 334, 443
BceAI ACGGC 4 cut(s) 170, 196, 313, 324
BciT130I CCWGG 2 cut(s) 89, 131
BcoDI GTCTC 2 cut(s) 8, 447
BfaI CTAG 2 cut(s) 6, 116
BfoI RGCGCY 1 cut(s) 186
BisI GCNGC 3 cut(s) 99, 257, 312
BlnI CCTAGG 1 cut(s) 115
BlsI GCNGC 3 cut(s) 100, 258, 313
Bme1390I CCNGG 2 cut(s) 89, 131
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 343
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 2 cut(s) 89, 131
BpmI CTGGAG 1 cut(s) 110
BsaHI GRCGYC 2 cut(s) 168, 183
BsaJI CCNNGG 3 cut(s) 115, 129, 130
BsaWI WCCGGW 1 cut(s) 273
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 1 cut(s) 94
BseBI CCWGG 2 cut(s) 89, 131
BseDI CCNNGG 3 cut(s) 115, 129, 130
BseLI CCNNNNNNNGG 1 cut(s) 94
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 1 cut(s) 268
Bsh1236I CGCG 1 cut(s) 288
Bsh1285I CGRYCG 2 cut(s) 278, 286
BshFI GGCC 4 cut(s) 98, 158, 335, 345
BshNI GGYRCC 1 cut(s) 182
BsiEI CGRYCG 2 cut(s) 278, 286
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 2 cut(s) 8, 447
BsnI GGCC 4 cut(s) 98, 158, 335, 345
BsoBI CYCGRG 1 cut(s) 11
BspACI CCGC 6 cut(s) 52, 99, 109, 286, 311, 389
BspANI GGCC 4 cut(s) 98, 158, 335, 345
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 1 cut(s) 184
BspT107I GGYRCC 1 cut(s) 182
BsrBI CCGCTC 1 cut(s) 111
BssECI CCNNGG 3 cut(s) 115, 129, 130
BssNI GRCGYC 2 cut(s) 168, 183
BssT1I CCWWGG 1 cut(s) 115
Bst2UI CCWGG 2 cut(s) 89, 131
Bst4CI ACNGT 3 cut(s) 293, 401, 407
BstACI GRCGYC 2 cut(s) 168, 183
BstC8I GCNNGC 2 cut(s) 96, 321
BstFNI CGCG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 1 cut(s) 185
BstMAI GTCTC 2 cut(s) 8, 447
BstMCI CGRYCG 2 cut(s) 278, 286
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstNI CCWGG 2 cut(s) 89, 131
BstSCI CCNGG 2 cut(s) 87, 129
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 1 cut(s) 268
BsuRI GGCC 4 cut(s) 98, 158, 335, 345
Cac8I GCNNGC 2 cut(s) 96, 321
CfoI GCGC 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 343
CseI GACGC 1 cut(s) 176
Csp6I GTAC 1 cut(s) 358
CviAII CATG 8 cut(s) 49, 61, 163, 217, 226, 262, 376, 439
CviQI GTAC 1 cut(s) 358
DinI GGCGCC 1 cut(s) 184
EaeI YGGCCR 3 cut(s) 96, 156, 333
EciI GGCGGA 1 cut(s) 404
Eco130I CCWWGG 1 cut(s) 115
Eco88I CYCGRG 1 cut(s) 11
EcoRII CCWGG 2 cut(s) 87, 129
EcoT14I CCWWGG 1 cut(s) 115
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 115
FaeI CATG 8 cut(s) 52, 64, 166, 220, 229, 265, 379, 442
FaiI YATR 9 cut(s) 50, 62, 164, 218, 227, 263, 305, 377, 440
FatI CATG 8 cut(s) 48, 60, 162, 216, 225, 261, 375, 438
Fnu4HI GCNGC 3 cut(s) 99, 257, 312
Fsp4HI GCNGC 3 cut(s) 99, 257, 312
FspBI CTAG 2 cut(s) 6, 116
GlaI GCGC 1 cut(s) 184
GluI GCNGC 3 cut(s) 99, 257, 312
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 4 cut(s) 98, 158, 335, 345
HapII CCGG 1 cut(s) 274
HgaI GACGC 1 cut(s) 176
HhaI GCGC 1 cut(s) 185
Hin1I GRCGYC 2 cut(s) 168, 183
Hin1II CATG 8 cut(s) 52, 64, 166, 220, 229, 265, 379, 442
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 2 cut(s) 65, 238
HpaII CCGG 1 cut(s) 274
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 2 cut(s) 208, 247
Hpy188I TCNGA 2 cut(s) 70, 373
Hpy188III TCNNGA 2 cut(s) 13, 444
Hpy8I GTNNAC 2 cut(s) 208, 247
Hpy99I CGWCG 2 cut(s) 236, 293
HpyCH4III ACNGT 3 cut(s) 293, 401, 407
HpyCH4IV ACGT 1 cut(s) 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpySE526I ACGT 1 cut(s) 356
Hsp92I GRCGYC 2 cut(s) 168, 183
Hsp92II CATG 8 cut(s) 52, 64, 166, 220, 229, 265, 379, 442
HspAI GCGC 1 cut(s) 183
KasI GGCGCC 1 cut(s) 182
LmnI GCTCC 4 cut(s) 91, 111, 116, 147
Lsp1109I GCAGC 1 cut(s) 268
MaeI CTAG 2 cut(s) 6, 116
MaeII ACGT 1 cut(s) 356
MaeIII GTNAC 2 cut(s) 293, 401
MbiI CCGCTC 1 cut(s) 111
MboII GAAGA 1 cut(s) 37
MlsI TGGCCA 2 cut(s) 158, 335
MluNI TGGCCA 2 cut(s) 158, 335
Mly113I GGCGCC 1 cut(s) 183
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 4 cut(s) 190, 259, 375, 379
Mox20I TGGCCA 2 cut(s) 158, 335
MscI TGGCCA 2 cut(s) 158, 335
MseI TTAA 3 cut(s) 36, 152, 460
MslI CAYNNNNRTG 3 cut(s) 47, 266, 437
Msp20I TGGCCA 2 cut(s) 158, 335
MspI CCGG 1 cut(s) 274
MspR9I CCNGG 2 cut(s) 89, 131
MvaI CCWGG 2 cut(s) 89, 131
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 320
NarI GGCGCC 1 cut(s) 183
NlaIII CATG 8 cut(s) 52, 64, 166, 220, 229, 265, 379, 442
NlaIV GGNNCC 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 11
PasI CCCWGGG 1 cut(s) 130
PfeI GAWTC 1 cut(s) 238
PflFI GACNNNGTC 1 cut(s) 407
PkrI GCNGC 3 cut(s) 100, 258, 313
PleI GAGTC 1 cut(s) 59
PluTI GGCGCC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 59
Psp6I CCWGG 2 cut(s) 87, 129
PspGI CCWGG 2 cut(s) 87, 129
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 1 cut(s) 343
PsyI GACNNNGTC 1 cut(s) 407
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
RseI CAYNNNNRTG 3 cut(s) 47, 266, 437
SaqAI TTAA 3 cut(s) 36, 152, 460
SatI GCNGC 3 cut(s) 99, 257, 312
Sau96I GGNCC 1 cut(s) 343
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 2 cut(s) 89, 131
SetI ASST 9 cut(s) 36, 90, 96, 144, 261, 270, 321, 359, 419
SfoI GGCGCC 1 cut(s) 184
Sfr274I CTCGAG 1 cut(s) 11
SlaI CTCGAG 1 cut(s) 11
SmiMI CAYNNNNRTG 3 cut(s) 47, 266, 437
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SsiI CCGC 6 cut(s) 52, 99, 109, 286, 311, 389
SspDI GGCGCC 1 cut(s) 182
SspMI CTAG 2 cut(s) 6, 116
StyD4I CCNGG 2 cut(s) 87, 129
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 3 cut(s) 293, 401, 407
TaiI ACGT 1 cut(s) 359
TaqI TCGA 6 cut(s) 12, 57, 195, 282, 329, 445
TauI GCSGC 2 cut(s) 101, 314
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 3 cut(s) 36, 152, 460
Tru9I TTAA 3 cut(s) 36, 152, 460
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 1 cut(s) 256
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 2 cut(s) 37, 242
Tth111I GACNNNGTC 1 cut(s) 407
XhoI CTCGAG 1 cut(s) 11
XmaJI CCTAGG 1 cut(s) 115
XspI CTAG 2 cut(s) 6, 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.