pycom14g18180

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005153
Physical Location & Seq
Forward (+)
19906612 .. 19907094
483 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g18180.1

Sequence Viewer

Length: 483 bp
ATGCCTGCACTACGAGTTGATCAAATCAACCAGCTAAGAAACATCTTTGCAAGATTCGACATTGTCTCCGACGGCAGCCTCACCGTCTTGGAGCTCGCCACGCTCCTCCGCTCGCTCGACCTCAAGCCCTCCGGTGACCAAGTCCACGTCCTCCTAGCCAATATGGATGCAAACGGCAATGGCTCCGTGGAATTCGACGAGCTGGTCAGTGCCATATTGCCCGGAATGGACGAGGAGATTTTGGTAAACCAAAAGCAACTCTTGGAGGTTTTTCGCTCTTTTGAACATGACGGCAACGGTGAAATAACTGCGGCAGAGCTGGCAGGGTCCATGGCGAAAATGGGGCAACCGTTGACGTATAAGGAGCTGAAGGAGATGATCAAAGAGGCTGATACGGATGGAGATGGTGTCATTAGTTTCAATGAATTTGCTACAATAATGGCAAAATCGGCATCGAGTTTTCTAGGCCTTGCTGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

161

Amino Acids

17.31

Weight (kDa)

4.37

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 10 - 72 4.6e-10 EF-hand domain pair
AIF-1 PF21008 78 - 156 7.4e-07 Allograft inflammatory factor 1
EF-hand_7 PF13499 85 - 147 3.2e-14 EF-hand domain pair
EF-hand_8 PF13833 99 - 147 1.4e-10 EF-hand domain pair
EF-hand_1 PF00036 122 - 149 1e-09 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
AccBSI CCGCTC 1 cut(s) 111
AciI CCGC 2 cut(s) 109, 311
AcsI RAATTY 2 cut(s) 191, 425
AcuI CTGAAG 1 cut(s) 389
AgsI TTSAA 2 cut(s) 284, 421
AjiI CACGTC 1 cut(s) 148
AluBI AGCT 5 cut(s) 34, 94, 202, 319, 367
AluI AGCT 5 cut(s) 34, 94, 202, 319, 367
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 70
AoxI GGCC 1 cut(s) 466
ApeKI GCWGC 2 cut(s) 75, 473
ApoI RAATTY 2 cut(s) 191, 425
AspS9I GGNCC 1 cut(s) 327
AsuC2I CCSGG 1 cut(s) 222
AsuHPI GGTGA 3 cut(s) 73, 146, 311
AvaII GGWCC 1 cut(s) 327
BanII GRGCYC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 87, 460
BccI CCATC 2 cut(s) 392, 398
BceAI ACGGC 3 cut(s) 88, 190, 307
BclI TGATCA 2 cut(s) 19, 378
BcnI CCSGG 1 cut(s) 222
BcoDI GTCTC 1 cut(s) 70
BfaI CTAG 2 cut(s) 155, 464
BisI GCNGC 3 cut(s) 76, 312, 474
BlsI GCNGC 3 cut(s) 77, 313, 475
Bme1390I CCNGG 1 cut(s) 222
Bme18I GGWCC 1 cut(s) 327
BmgBI CACGTC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 327
BmiI GGNNCC 2 cut(s) 184, 328
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 2 cut(s) 157, 461
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 186, 330
BsaWI WCCGGW 1 cut(s) 131
BsaXI ACNNNNNCTCC 4 cut(s) 50, 80, 393, 423
Bse3DI GCAATG 1 cut(s) 184
BseDI CCNNGG 2 cut(s) 186, 330
BseGI GGATG 2 cut(s) 172, 403
BseMI GCAATG 1 cut(s) 184
BseRI GAGGAG 2 cut(s) 95, 248
BseXI GCAGC 2 cut(s) 87, 460
BshFI GGCC 1 cut(s) 468
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 2 cut(s) 132, 222
BsmAI GTCTC 1 cut(s) 70
BsnI GGCC 1 cut(s) 468
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 19, 378
Bsp19I CCATGG 1 cut(s) 330
BspACI CCGC 2 cut(s) 109, 311
BspANI GGCC 1 cut(s) 468
BspHI TCATGA 1 cut(s) 479
BspLI GGNNCC 2 cut(s) 184, 328
BsrBI CCGCTC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 184
BssECI CCNNGG 2 cut(s) 186, 330
BssMI GATC 2 cut(s) 19, 378
BssT1I CCWWGG 1 cut(s) 330
Bst4CI ACNGT 3 cut(s) 85, 299, 351
BstC8I GCNNGC 4 cut(s) 6, 96, 113, 321
BstDEI CTNAG 1 cut(s) 35
BstDSI CCRYGG 2 cut(s) 186, 330
BstEII GGTNACC 1 cut(s) 134
BstF5I GGATG 2 cut(s) 172, 403
BstKTI GATC 2 cut(s) 22, 381
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 2 cut(s) 19, 378
BstMWI GCNNNNNNNGC 3 cut(s) 100, 320, 449
BstPI GGTNACC 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 220
BstV1I GCAGC 2 cut(s) 87, 460
BsuRI GGCC 1 cut(s) 468
BtgI CCRYGG 2 cut(s) 186, 330
BtrI CACGTC 1 cut(s) 148
BtsCI GGATG 2 cut(s) 172, 403
BtsIMutI CAGTG 1 cut(s) 214
Cac8I GCNNGC 4 cut(s) 6, 96, 113, 321
CciI TCATGA 1 cut(s) 479
Cfr13I GGNCC 1 cut(s) 327
CviAII CATG 3 cut(s) 287, 331, 480
DdeI CTNAG 1 cut(s) 35
DpnI GATC 2 cut(s) 21, 380
DpnII GATC 2 cut(s) 19, 378
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 330
Eco147I AGGCCT 1 cut(s) 468
Eco24I GRGCYC 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 389
Eco91I GGTNACC 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 94
EcoO65I GGTNACC 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 3 cut(s) 290, 334, 483
FaiI YATR 6 cut(s) 164, 215, 288, 332, 360, 481
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FatI CATG 3 cut(s) 286, 330, 479
FbaI TGATCA 2 cut(s) 19, 378
Fnu4HI GCNGC 3 cut(s) 76, 312, 474
FokI GGATG 2 cut(s) 179, 410
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 3 cut(s) 76, 312, 474
FspBI CTAG 2 cut(s) 155, 464
GluI GCNGC 3 cut(s) 76, 312, 474
HaeIII GGCC 1 cut(s) 468
HapII CCGG 2 cut(s) 132, 222
Hin1II CATG 3 cut(s) 290, 334, 483
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HinfI GANTC 1 cut(s) 54
HpaII CCGG 2 cut(s) 132, 222
HphI GGTGA 3 cut(s) 73, 146, 311
Hpy166II GTNNAC 3 cut(s) 145, 247, 354
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 1 cut(s) 480
Hpy8I GTNNAC 3 cut(s) 145, 247, 354
Hpy99I CGWCG 2 cut(s) 74, 200
HpyAV CCTTC 1 cut(s) 364
HpyCH4III ACNGT 3 cut(s) 85, 299, 351
HpyCH4IV ACGT 2 cut(s) 147, 356
HpyCH4V TGCA 3 cut(s) 8, 50, 170
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 320, 449
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 2 cut(s) 147, 356
Hsp92II CATG 3 cut(s) 290, 334, 483
Ksp22I TGATCA 2 cut(s) 19, 378
Kzo9I GATC 2 cut(s) 19, 378
LmnI GCTCC 4 cut(s) 91, 108, 188, 364
LpnPI CCDG 7 cut(s) 18, 44, 145, 188, 235, 305, 309
Lsp1109I GCAGC 2 cut(s) 87, 460
LweI GCATC 2 cut(s) 157, 461
MaeI CTAG 2 cut(s) 155, 464
MaeII ACGT 2 cut(s) 147, 356
MaeIII GTNAC 1 cut(s) 134
MalI GATC 2 cut(s) 21, 380
MbiI CCGCTC 1 cut(s) 111
MboI GATC 2 cut(s) 19, 378
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 2 cut(s) 191, 425
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 8 cut(s) 89, 116, 131, 139, 161, 226, 259, 379
MspI CCGG 2 cut(s) 132, 222
MspR9I CCNGG 1 cut(s) 222
MwoI GCNNNNNNNGC 3 cut(s) 100, 320, 449
NciI CCSGG 1 cut(s) 222
NcoI CCATGG 1 cut(s) 330
NdeII GATC 2 cut(s) 19, 378
NlaIII CATG 3 cut(s) 290, 334, 483
NlaIV GGNNCC 2 cut(s) 184, 328
NmuCI GTSAC 1 cut(s) 134
PagI TCATGA 1 cut(s) 479
PceI AGGCCT 1 cut(s) 468
PfeI GAWTC 1 cut(s) 54
PflFI GACNNNGTC 2 cut(s) 62, 140
PkrI GCNGC 3 cut(s) 77, 313, 475
Psp124BI GAGCTC 1 cut(s) 96
PspEI GGTNACC 1 cut(s) 134
PspN4I GGNNCC 2 cut(s) 184, 328
PspPI GGNCC 1 cut(s) 327
PsyI GACNNNGTC 2 cut(s) 62, 140
SacI GAGCTC 1 cut(s) 96
SatI GCNGC 3 cut(s) 76, 312, 474
Sau3AI GATC 2 cut(s) 19, 378
Sau96I GGNCC 1 cut(s) 327
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 1 cut(s) 96
SetI ASST 9 cut(s) 36, 96, 123, 150, 204, 270, 321, 359, 369
SfaNI GCATC 2 cut(s) 157, 461
SinI GGWCC 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 2 cut(s) 191, 425
SseBI AGGCCT 1 cut(s) 468
SsiI CCGC 2 cut(s) 109, 311
SspMI CTAG 2 cut(s) 155, 464
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 1 cut(s) 468
StyD4I CCNGG 1 cut(s) 220
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 3 cut(s) 85, 299, 351
TaiI ACGT 2 cut(s) 150, 359
TaqI TCGA 4 cut(s) 57, 117, 195, 455
TasI AATT 2 cut(s) 191, 425
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 1 cut(s) 54
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 2 cut(s) 75, 473
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 1 cut(s) 438
TspGWI ACGGA 2 cut(s) 175, 410
TspRI CASTG 1 cut(s) 214
Tth111I GACNNNGTC 2 cut(s) 62, 140
VpaK11BI GGWCC 1 cut(s) 327
XapI RAATTY 2 cut(s) 191, 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 337
XspI CTAG 2 cut(s) 155, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.