MD06G1209000.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005153
Physical Location & Seq
Forward (+)
34255417 .. 34255899
483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1209000.v1.1.491

Sequence Viewer

Length: 483 bp
ATGCCTGCATTACGAGTTGATCAACTCAACCAGCTACGAGACATCTTTGCCAGATTCGACATGGACTCTGACGGCAGCCTCACCATCTTGGAGCTTGCCGCTCTCCTCCGCTCGCTCGGCCTTAAGCCCTCGGGGGACCAAATCCACGTCCTCCTAGCCAACATGGACGCAAACGGCAATGGTTCCGTGGAGTTCGACGAGTTGGTCAGTGCCATATTGCCCGATATGAACGAGGAGATTTTGGTAAACCAAGAGCAACTCTTGGAGGTTTTTCGCTCTTTTGATCGTGACGGTAATGGGTACATAACTGCGGCAGAGCTGGCAGGGTCCATGGCGAAAATGGGACAACCGTTGACGTATAAGGAGCTGACGGAGATGATCAAAGAGGCTGATACGGATGGAGATGGTGTCATTAGCTTCAATGAATTTTCTACAATAATGGCGAAATCGGCATCGAGTTTTCTAGGCCTTGCTCCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

161

Amino Acids

17.39

Weight (kDa)

4.18

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 10 - 72 4.4e-12 EF-hand domain pair
EF-hand_6 PF13405 11 - 40 5.9e-06 EF-hand domain
AIF-1 PF21008 77 - 155 5.8e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 85 - 147 1.5e-16 EF-hand domain pair
EF-hand_1 PF00036 87 - 113 5.5e-06 EF hand domain
EF-hand_6 PF13405 87 - 114 1.9e-06 EF-hand domain
EF-hand_8 PF13833 102 - 147 8.2e-10 EF-hand domain pair
EF-hand_1 PF00036 122 - 149 9.7e-09 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
AccBSI CCGCTC 2 cut(s) 101, 111
AciI CCGC 3 cut(s) 99, 109, 311
AcsI RAATTY 1 cut(s) 425
AfaI GTAC 1 cut(s) 302
AflII CTTAAG 1 cut(s) 122
AgsI TTSAA 1 cut(s) 421
AjiI CACGTC 1 cut(s) 148
AluBI AGCT 5 cut(s) 34, 94, 319, 367, 417
AluI AGCT 5 cut(s) 34, 94, 319, 367, 417
Alw26I GTCTC 1 cut(s) 33
Ama87I CYCGRG 1 cut(s) 130
AoxI GGCC 2 cut(s) 118, 466
ApeKI GCWGC 1 cut(s) 75
ApoI RAATTY 1 cut(s) 425
AspS9I GGNCC 2 cut(s) 136, 327
AsuHPI GGTGA 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 130
AvaII GGWCC 2 cut(s) 136, 327
BbvI GCAGC 1 cut(s) 87
BccI CCATC 3 cut(s) 92, 392, 398
BceAI ACGGC 2 cut(s) 88, 190
BclI TGATCA 2 cut(s) 19, 378
BcoDI GTCTC 1 cut(s) 33
BfaI CTAG 2 cut(s) 155, 464
BfrI CTTAAG 1 cut(s) 122
BisI GCNGC 3 cut(s) 76, 99, 312
BlsI GCNGC 3 cut(s) 77, 100, 313
Bme18I GGWCC 2 cut(s) 136, 327
BmeT110I CYCGRG 1 cut(s) 130
BmgBI CACGTC 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 136, 327
BmiI GGNNCC 3 cut(s) 137, 184, 328
BmsI GCATC 1 cut(s) 461
BsaJI CCNNGG 3 cut(s) 129, 186, 330
BsaXI ACNNNNNCTCC 2 cut(s) 393, 423
Bse3DI GCAATG 1 cut(s) 184
BseDI CCNNGG 3 cut(s) 129, 186, 330
BseGI GGATG 1 cut(s) 403
BseMI GCAATG 1 cut(s) 184
BseRI GAGGAG 2 cut(s) 95, 248
BseXI GCAGC 1 cut(s) 87
BshFI GGCC 2 cut(s) 120, 468
BsiHKCI CYCGRG 1 cut(s) 130
BslFI GGGAC 2 cut(s) 149, 357
BsmAI GTCTC 1 cut(s) 33
BsmFI GGGAC 2 cut(s) 149, 357
BsnI GGCC 2 cut(s) 120, 468
BsoBI CYCGRG 1 cut(s) 130
Bsp143I GATC 3 cut(s) 19, 283, 378
Bsp19I CCATGG 1 cut(s) 330
BspACI CCGC 3 cut(s) 99, 109, 311
BspANI GGCC 2 cut(s) 120, 468
BspHI TCATGA 1 cut(s) 479
BspLI GGNNCC 3 cut(s) 137, 184, 328
BspTI CTTAAG 1 cut(s) 122
BsrBI CCGCTC 2 cut(s) 101, 111
BsrDI GCAATG 1 cut(s) 184
BssECI CCNNGG 3 cut(s) 129, 186, 330
BssMI GATC 3 cut(s) 19, 283, 378
BssT1I CCWWGG 1 cut(s) 330
Bst4CI ACNGT 2 cut(s) 293, 351
BstAFI CTTAAG 1 cut(s) 122
BstC8I GCNNGC 4 cut(s) 6, 96, 113, 321
BstDSI CCRYGG 2 cut(s) 186, 330
BstF5I GGATG 1 cut(s) 403
BstKTI GATC 3 cut(s) 22, 286, 381
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 3 cut(s) 19, 283, 378
BstMWI GCNNNNNNNGC 3 cut(s) 117, 320, 449
BstV1I GCAGC 1 cut(s) 87
BsuRI GGCC 2 cut(s) 120, 468
BtgI CCRYGG 2 cut(s) 186, 330
BtrI CACGTC 1 cut(s) 148
BtsCI GGATG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 214
Cac8I GCNNGC 4 cut(s) 6, 96, 113, 321
CciI TCATGA 1 cut(s) 479
Cfr13I GGNCC 2 cut(s) 136, 327
CseI GACGC 1 cut(s) 176
Csp6I GTAC 1 cut(s) 301
CviAII CATG 4 cut(s) 61, 163, 331, 480
CviQI GTAC 1 cut(s) 301
DpnI GATC 3 cut(s) 21, 285, 380
DpnII GATC 3 cut(s) 19, 283, 378
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
Eco130I CCWWGG 1 cut(s) 330
Eco147I AGGCCT 1 cut(s) 468
Eco47I GGWCC 2 cut(s) 136, 327
Eco88I CYCGRG 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 4 cut(s) 64, 166, 334, 483
FaiI YATR 8 cut(s) 62, 164, 215, 227, 305, 332, 360, 481
FaqI GGGAC 2 cut(s) 149, 357
FatI CATG 4 cut(s) 60, 162, 330, 479
FbaI TGATCA 2 cut(s) 19, 378
Fnu4HI GCNGC 3 cut(s) 76, 99, 312
FokI GGATG 1 cut(s) 410
Fsp4HI GCNGC 3 cut(s) 76, 99, 312
FspBI CTAG 2 cut(s) 155, 464
GluI GCNGC 3 cut(s) 76, 99, 312
HaeIII GGCC 2 cut(s) 120, 468
HgaI GACGC 1 cut(s) 176
Hin1II CATG 4 cut(s) 64, 166, 334, 483
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HinfI GANTC 2 cut(s) 54, 65
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 247, 354
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 2 cut(s) 287, 480
Hpy8I GTNNAC 2 cut(s) 247, 354
Hpy99I CGWCG 1 cut(s) 200
HpyCH4III ACNGT 2 cut(s) 293, 351
HpyCH4IV ACGT 2 cut(s) 147, 356
HpyCH4V TGCA 1 cut(s) 8
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 320, 449
HpySE526I ACGT 2 cut(s) 147, 356
Hsp92II CATG 4 cut(s) 64, 166, 334, 483
Ksp22I TGATCA 2 cut(s) 19, 378
Kzo9I GATC 3 cut(s) 19, 283, 378
LmnI GCTCC 3 cut(s) 91, 364, 478
LpnPI CCDG 5 cut(s) 18, 44, 64, 305, 309
Lsp1109I GCAGC 1 cut(s) 87
LweI GCATC 1 cut(s) 461
MaeI CTAG 2 cut(s) 155, 464
MaeII ACGT 2 cut(s) 147, 356
MaeIII GTNAC 1 cut(s) 287
MalI GATC 3 cut(s) 21, 285, 380
MbiI CCGCTC 2 cut(s) 101, 111
MboI GATC 3 cut(s) 19, 283, 378
MluCI AATT 1 cut(s) 425
MlyI GAGTC 1 cut(s) 59
MnlI CCTC 7 cut(s) 89, 116, 139, 161, 226, 259, 379
MseI TTAA 1 cut(s) 123
MspCI CTTAAG 1 cut(s) 122
MwoI GCNNNNNNNGC 3 cut(s) 117, 320, 449
NcoI CCATGG 1 cut(s) 330
NdeII GATC 3 cut(s) 19, 283, 378
NlaIII CATG 4 cut(s) 64, 166, 334, 483
NlaIV GGNNCC 3 cut(s) 137, 184, 328
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 287
PagI TCATGA 1 cut(s) 479
PceI AGGCCT 1 cut(s) 468
PfeI GAWTC 1 cut(s) 54
PkrI GCNGC 3 cut(s) 77, 100, 313
PleI GAGTC 1 cut(s) 59
PpsI GAGTC 1 cut(s) 59
PspN4I GGNNCC 3 cut(s) 137, 184, 328
PspPI GGNCC 2 cut(s) 136, 327
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 3 cut(s) 76, 99, 312
Sau3AI GATC 3 cut(s) 19, 283, 378
Sau96I GGNCC 2 cut(s) 136, 327
SchI GAGTC 1 cut(s) 59
SetI ASST 8 cut(s) 36, 96, 150, 270, 321, 359, 369, 419
SfaNI GCATC 1 cut(s) 461
SinI GGWCC 2 cut(s) 136, 327
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 425
SseBI AGGCCT 1 cut(s) 468
SsiI CCGC 3 cut(s) 99, 109, 311
SspMI CTAG 2 cut(s) 155, 464
StuI AGGCCT 1 cut(s) 468
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 2 cut(s) 293, 351
TaiI ACGT 2 cut(s) 150, 359
TaqI TCGA 3 cut(s) 57, 195, 455
TasI AATT 1 cut(s) 425
TauI GCSGC 2 cut(s) 101, 314
TfiI GAWTC 1 cut(s) 54
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 1 cut(s) 75
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 2 cut(s) 242, 438
TspGWI ACGGA 3 cut(s) 175, 386, 410
TspRI CASTG 1 cut(s) 214
Vha464I CTTAAG 1 cut(s) 122
VpaK11BI GGWCC 2 cut(s) 136, 327
XapI RAATTY 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 2 cut(s) 58, 337
XspI CTAG 2 cut(s) 155, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.