RchiOBHm_Chr4g0436421

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005153
Physical Location & Seq
Reverse (-)
59730746 .. 59731222
477 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40475

Sequence Viewer

Length: 477 bp
ATGATGGCTAGGCTCGAGACAGACCAGCTCAAACAGCTTAAAGACATCTTCATGCGGTTCGACATGGACTCCGACGGCAGCCTAACCCACCTGGAGCTGGCCGCCCTGCTCCGCTCCCTAGGCGTCAAGCCCCAGGGAGACCAGCTCCACATGTTAATGGCCAACATGGACGCCAATGGAAACGGCGCCGTTGAGTTCGAGGAGTTGGTAAGCGCCATCATGCCGAACATGAACGACGAGATTCTTGTCAACCAAGAGCAGCTCATGGAGGTGTTCCGGTCGTTCGACCGCGACGGTAACGGCTACATAACGGCGGCAGAGCTGGCCGGGTCGATGGCCAAGATGGGCCACCCCTTGACGTACAGGGAGTTGTCAGATATGATGAGGGAGGCGGACACCAACGGTGACGGCGTCATCAGCTTCAACGAGTTCGCCACCATCATGTCTCGGTCCGCCGCTAACTTTCTCGGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

158

Amino Acids

17.56

Weight (kDa)

4.43

Isoelectric Point (pI)

27.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 11 - 73 6.7e-11 EF-hand domain pair
EF-hand_7 PF13499 86 - 149 2.7e-16 EF-hand domain pair
EF-hand_6 PF13405 87 - 115 9.5e-07 EF-hand domain
EF-hand_1 PF00036 88 - 114 3.1e-06 EF hand domain
EF-hand_8 PF13833 103 - 148 1.6e-08 EF-hand domain pair
EF-hand_1 PF00036 123 - 150 1.3e-08 EF hand domain
EF-hand_5 PF13202 126 - 148 6.5e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 185
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 1 cut(s) 291
AciI CCGC 8 cut(s) 55, 102, 112, 289, 314, 392, 453, 456
AcoI YGGCCR 4 cut(s) 99, 159, 324, 336
AcyI GRCGYC 4 cut(s) 123, 171, 186, 411
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 1 cut(s) 97
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 1 cut(s) 424
AjnI CCWGG 2 cut(s) 90, 132
AluBI AGCT 7 cut(s) 28, 37, 97, 145, 262, 322, 420
AluI AGCT 7 cut(s) 28, 37, 97, 145, 262, 322, 420
Alw26I GTCTC 3 cut(s) 11, 132, 450
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 5 cut(s) 99, 159, 324, 336, 346
ApeKI GCWGC 2 cut(s) 78, 259
AspA2I CCTAGG 1 cut(s) 118
AspLEI GCGC 2 cut(s) 188, 215
AspS9I GGNCC 2 cut(s) 346, 450
AsuC2I CCSGG 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 1 cut(s) 450
AvrII CCTAGG 1 cut(s) 118
BalI TGGCCA 2 cut(s) 161, 338
BanI GGYRCC 1 cut(s) 185
BbvI GCAGC 2 cut(s) 90, 271
BccI CCATC 4 cut(s) 224, 328, 337, 446
BceAI ACGGC 6 cut(s) 91, 173, 199, 316, 327, 424
BciT130I CCWGG 2 cut(s) 92, 134
BcnI CCSGG 1 cut(s) 328
BcoDI GTCTC 3 cut(s) 11, 132, 450
BfaI CTAG 2 cut(s) 9, 119
BfoI RGCGCY 2 cut(s) 189, 216
BisI GCNGC 5 cut(s) 79, 102, 260, 315, 456
BlnI CCTAGG 1 cut(s) 118
BlsI GCNGC 5 cut(s) 80, 103, 261, 316, 457
Bme1390I CCNGG 3 cut(s) 92, 134, 328
Bme18I GGWCC 1 cut(s) 450
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 2 cut(s) 346, 450
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 3 cut(s) 92, 134, 328
BplI GAGNNNNNCTC 2 cut(s) 129, 161
BpmI CTGGAG 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 328
BsaHI GRCGYC 4 cut(s) 123, 171, 186, 411
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 3 cut(s) 118, 132, 133
BsaWI WCCGGW 1 cut(s) 276
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bsc4I CCNNNNNNNGG 1 cut(s) 97
BseBI CCWGG 2 cut(s) 92, 134
BseDI CCNNGG 3 cut(s) 118, 132, 133
BseLI CCNNNNNNNGG 1 cut(s) 97
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 2 cut(s) 90, 271
Bsh1236I CGCG 1 cut(s) 291
Bsh1285I CGRYCG 2 cut(s) 281, 289
BshFI GGCC 5 cut(s) 101, 161, 326, 338, 348
BshNI GGYRCC 1 cut(s) 185
BsiEI CGRYCG 2 cut(s) 281, 289
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 2 cut(s) 277, 327
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 3 cut(s) 11, 132, 450
BsnI GGCC 5 cut(s) 101, 161, 326, 338, 348
Bso31I GGTCTC 1 cut(s) 132
BsoBI CYCGRG 1 cut(s) 14
BspACI CCGC 8 cut(s) 55, 102, 112, 289, 314, 392, 453, 456
BspANI GGCC 5 cut(s) 101, 161, 326, 338, 348
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 187
BspT107I GGYRCC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 132
BsrBI CCGCTC 1 cut(s) 114
BssECI CCNNGG 3 cut(s) 118, 132, 133
BssNI GRCGYC 4 cut(s) 123, 171, 186, 411
BssT1I CCWWGG 1 cut(s) 118
Bst2UI CCWGG 2 cut(s) 92, 134
Bst4CI ACNGT 2 cut(s) 296, 404
BstACI GRCGYC 4 cut(s) 123, 171, 186, 411
BstC8I GCNNGC 2 cut(s) 99, 324
BstFNI CGCG 1 cut(s) 291
BstH2I RGCGCY 2 cut(s) 189, 216
BstHHI GCGC 2 cut(s) 188, 215
BstMAI GTCTC 3 cut(s) 11, 132, 450
BstMCI CGRYCG 2 cut(s) 281, 289
BstMWI GCNNNNNNNGC 4 cut(s) 34, 120, 323, 417
BstNI CCWGG 2 cut(s) 92, 134
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 3 cut(s) 90, 132, 326
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 90, 271
BsuRI GGCC 5 cut(s) 101, 161, 326, 338, 348
Cac8I GCNNGC 2 cut(s) 99, 324
CfoI GCGC 2 cut(s) 188, 215
Cfr13I GGNCC 2 cut(s) 346, 450
CpoI CGGWCCG 1 cut(s) 450
CseI GACGC 3 cut(s) 112, 179, 400
Csp6I GTAC 1 cut(s) 361
CspI CGGWCCG 1 cut(s) 450
CviAII CATG 8 cut(s) 52, 64, 151, 166, 220, 229, 265, 442
CviQI GTAC 1 cut(s) 361
DinI GGCGCC 1 cut(s) 187
EaeI YGGCCR 4 cut(s) 99, 159, 324, 336
EciI GGCGGA 2 cut(s) 407, 442
Eco130I CCWWGG 1 cut(s) 118
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 450
Eco88I CYCGRG 1 cut(s) 14
EcoRII CCWGG 2 cut(s) 90, 132
EcoT14I CCWWGG 1 cut(s) 118
EgeI GGCGCC 1 cut(s) 187
EheI GGCGCC 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 8 cut(s) 55, 67, 154, 169, 223, 232, 268, 445
FatI CATG 8 cut(s) 51, 63, 150, 165, 219, 228, 264, 441
Fnu4HI GCNGC 5 cut(s) 79, 102, 260, 315, 456
Fsp4HI GCNGC 5 cut(s) 79, 102, 260, 315, 456
FspBI CTAG 2 cut(s) 9, 119
GlaI GCGC 2 cut(s) 187, 214
GluI GCNGC 5 cut(s) 79, 102, 260, 315, 456
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 2 cut(s) 189, 216
HaeIII GGCC 5 cut(s) 101, 161, 326, 338, 348
HapII CCGG 2 cut(s) 277, 327
HgaI GACGC 3 cut(s) 112, 179, 400
HhaI GCGC 2 cut(s) 188, 215
Hin1I GRCGYC 4 cut(s) 123, 171, 186, 411
Hin1II CATG 8 cut(s) 55, 67, 154, 169, 223, 232, 268, 445
Hin6I GCGC 2 cut(s) 186, 213
HinP1I GCGC 2 cut(s) 186, 213
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 3 cut(s) 68, 241, 471
HpaII CCGG 2 cut(s) 277, 327
HphI GGTGA 1 cut(s) 416
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 4 cut(s) 73, 376, 470, 476
Hpy188III TCNNGA 1 cut(s) 16
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 3 cut(s) 77, 239, 296
HpyCH4III ACNGT 2 cut(s) 296, 404
HpyCH4IV ACGT 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 120, 323, 417
HpySE526I ACGT 1 cut(s) 359
Hsp92I GRCGYC 4 cut(s) 123, 171, 186, 411
Hsp92II CATG 8 cut(s) 55, 67, 154, 169, 223, 232, 268, 445
HspAI GCGC 2 cut(s) 186, 213
KasI GGCGCC 1 cut(s) 185
LmnI GCTCC 4 cut(s) 94, 114, 119, 150
Lsp1109I GCAGC 2 cut(s) 90, 271
MaeI CTAG 2 cut(s) 9, 119
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 2 cut(s) 296, 404
MbiI CCGCTC 1 cut(s) 114
MboII GAAGA 1 cut(s) 40
MlsI TGGCCA 2 cut(s) 161, 338
MluNI TGGCCA 2 cut(s) 161, 338
Mly113I GGCGCC 1 cut(s) 186
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 4 cut(s) 193, 262, 378, 382
Mox20I TGGCCA 2 cut(s) 161, 338
MscI TGGCCA 2 cut(s) 161, 338
MseI TTAA 2 cut(s) 39, 155
MslI CAYNNNNRTG 4 cut(s) 50, 155, 269, 440
Msp20I TGGCCA 2 cut(s) 161, 338
MspI CCGG 2 cut(s) 277, 327
MspR9I CCNGG 3 cut(s) 92, 134, 328
MvaI CCWGG 2 cut(s) 92, 134
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 4 cut(s) 34, 120, 323, 417
NarI GGCGCC 1 cut(s) 186
NciI CCSGG 1 cut(s) 328
NlaIII CATG 8 cut(s) 55, 67, 154, 169, 223, 232, 268, 445
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 404
NspI RCATGY 1 cut(s) 154
PaeR7I CTCGAG 1 cut(s) 14
PasI CCCWGGG 1 cut(s) 133
PciI ACATGT 1 cut(s) 150
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PfeI GAWTC 2 cut(s) 241, 471
PflFI GACNNNGTC 1 cut(s) 410
PkrI GCNGC 5 cut(s) 80, 103, 261, 316, 457
PleI GAGTC 1 cut(s) 62
PluTI GGCGCC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 62
PscI ACATGT 1 cut(s) 150
Psp6I CCWGG 2 cut(s) 90, 132
PspGI CCWGG 2 cut(s) 90, 132
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 2 cut(s) 346, 450
PsyI GACNNNGTC 1 cut(s) 410
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 4 cut(s) 50, 155, 269, 440
Rsr2I CGGWCCG 1 cut(s) 450
RsrII CGGWCCG 1 cut(s) 450
SaqAI TTAA 2 cut(s) 39, 155
SatI GCNGC 5 cut(s) 79, 102, 260, 315, 456
Sau96I GGNCC 2 cut(s) 346, 450
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 3 cut(s) 92, 134, 328
SfoI GGCGCC 1 cut(s) 187
Sfr274I CTCGAG 1 cut(s) 14
SinI GGWCC 1 cut(s) 450
SlaI CTCGAG 1 cut(s) 14
SmiMI CAYNNNNRTG 4 cut(s) 50, 155, 269, 440
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
SsiI CCGC 8 cut(s) 55, 102, 112, 289, 314, 392, 453, 456
SspDI GGCGCC 1 cut(s) 185
SspMI CTAG 2 cut(s) 9, 119
StyD4I CCNGG 3 cut(s) 90, 132, 326
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 2 cut(s) 296, 404
TaiI ACGT 1 cut(s) 362
TaqI TCGA 5 cut(s) 15, 60, 198, 285, 332
TaqII GACCGA 1 cut(s) 438
TauI GCSGC 3 cut(s) 104, 317, 458
TfiI GAWTC 2 cut(s) 241, 471
Tru1I TTAA 2 cut(s) 39, 155
Tru9I TTAA 2 cut(s) 39, 155
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 2 cut(s) 78, 259
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 2 cut(s) 40, 245
Tth111I GACNNNGTC 1 cut(s) 410
VpaK11BI GGWCC 1 cut(s) 450
XceI RCATGY 1 cut(s) 154
XhoI CTCGAG 1 cut(s) 14
XmaJI CCTAGG 1 cut(s) 118
XspI CTAG 2 cut(s) 9, 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.