Rroxscaffold_3G00269760

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005153
Physical Location & Seq
Reverse (-)
62524675 .. 62525157
483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269760.1

Sequence Viewer

Length: 483 bp
ATGCCTGCATTACAAGTAGAGCAACTCAACCAACTAAGGGCCATCTTCGCAAGATTCGACATGGACTCAGATGGCAGCCTCACCATTCTAGAGCTCGGGGCGCTTCTCCGGTCCCTAGGACTCAGGCCCTCAGGTGACCAAATGCATGTTCTGCTAAGCAACATTGACGCAAACGGCAATGGATCTGTTGAATTTGATGAGTTGGTCAAGGCCATTTTGCCTGACATGAATGAAGAGATCTTTATAAACCAGCAGCAACTCATGGAGGTTTTTCAGTCCTTTGATCGCGACGGCAATGGCTACATTACTGCAGCAGAGCTGGCAAAATCAATGGCCAAAATGGGCCAACCGTTGTCGTATAAGGAGCTCACAGAGATGATCAAAGAGGCTGATACAGATGGAGATGGTGTGATCAGCTTTAATGAATTTGCAACTATAATGGCAAAGTCTGCCTCAGGTTATCTAGGCCTTGGTCTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

160

Amino Acids

17.47

Weight (kDa)

4.26

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 10 - 72 1e-10 EF-hand domain pair
EF-hand_6 PF13405 11 - 40 9.2e-06 EF-hand domain
AIF-1 PF21008 81 - 155 1e-05 Allograft inflammatory factor 1
EF-hand_7 PF13499 85 - 147 1e-18 EF-hand domain pair
EF-hand_6 PF13405 86 - 115 4.7e-07 EF-hand domain
EF-hand_1 PF00036 87 - 114 1.9e-06 EF hand domain
EF-hand_8 PF13833 99 - 147 2.1e-12 EF-hand domain pair
EF-hand_1 PF00036 122 - 149 7.7e-09 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 245
AccII CGCG 1 cut(s) 288
AclWI GGATC 1 cut(s) 190
AcoI YGGCCR 1 cut(s) 333
AcsI RAATTY 2 cut(s) 191, 425
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 1 cut(s) 191
AjuI GAANNNNNNNTTGG 2 cut(s) 132, 164
AluBI AGCT 4 cut(s) 94, 319, 367, 417
AluI AGCT 4 cut(s) 94, 319, 367, 417
Alw21I GWGCWC 2 cut(s) 96, 369
Alw26I GTCTC 1 cut(s) 479
AlwI GGATC 1 cut(s) 190
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 6 cut(s) 39, 125, 210, 333, 343, 466
ApeKI GCWGC 3 cut(s) 75, 253, 311
ApoI RAATTY 2 cut(s) 191, 425
AspA2I CCTAGG 1 cut(s) 115
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 4 cut(s) 39, 111, 126, 343
AsuHPI GGTGA 2 cut(s) 73, 146
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 1 cut(s) 111
AvrII CCTAGG 1 cut(s) 115
AxyI CCTNAGG 2 cut(s) 130, 454
BalI TGGCCA 1 cut(s) 335
BanII GRGCYC 2 cut(s) 96, 369
Bbv12I GWGCWC 2 cut(s) 96, 369
BbvI GCAGC 3 cut(s) 87, 265, 323
BccI CCATC 4 cut(s) 50, 65, 392, 398
BceAI ACGGC 2 cut(s) 190, 307
BclI TGATCA 2 cut(s) 378, 411
BcoDI GTCTC 1 cut(s) 479
BfaI CTAG 3 cut(s) 89, 116, 464
BfmI CTRYAG 1 cut(s) 309
BfoI RGCGCY 1 cut(s) 104
BglII AGATCT 1 cut(s) 237
BisI GCNGC 3 cut(s) 76, 254, 312
BlnI CCTAGG 1 cut(s) 115
BlpI GCTNAGC 1 cut(s) 155
BlsI GCNGC 3 cut(s) 77, 255, 313
Bme18I GGWCC 1 cut(s) 111
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 4 cut(s) 39, 111, 126, 343
BmiI GGNNCC 1 cut(s) 113
Bpu1102I GCTNAGC 1 cut(s) 155
BsaI GGTCTC 1 cut(s) 479
BsaJI CCNNGG 2 cut(s) 115, 469
BsaWI WCCGGW 1 cut(s) 108
BsaXI ACNNNNNCTCC 2 cut(s) 393, 423
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse21I CCTNAGG 2 cut(s) 130, 454
Bse3DI GCAATG 2 cut(s) 184, 301
BseDI CCNNGG 2 cut(s) 115, 469
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMI GCAATG 2 cut(s) 184, 301
BseMII CTCAG 4 cut(s) 81, 136, 144, 468
BseXI GCAGC 3 cut(s) 87, 265, 323
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 6 cut(s) 41, 127, 212, 335, 345, 468
BsiHKAI GWGCWC 2 cut(s) 96, 369
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 1 cut(s) 109
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 6 cut(s) 41, 127, 212, 335, 345, 468
Bso31I GGTCTC 1 cut(s) 479
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 96, 369
Bsp143I GATC 5 cut(s) 182, 237, 283, 378, 411
Bsp1720I GCTNAGC 1 cut(s) 155
Bsp68I TCGCGA 1 cut(s) 288
BspANI GGCC 6 cut(s) 41, 127, 212, 335, 345, 468
BspCNI CTCAG 4 cut(s) 80, 135, 143, 467
BspFNI CGCG 1 cut(s) 288
BspHI TCATGA 1 cut(s) 479
BspLI GGNNCC 1 cut(s) 113
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 1 cut(s) 190
BspTNI GGTCTC 1 cut(s) 479
BsrDI GCAATG 2 cut(s) 184, 301
BssECI CCNNGG 2 cut(s) 115, 469
BssMI GATC 5 cut(s) 182, 237, 283, 378, 411
BssT1I CCWWGG 2 cut(s) 115, 469
Bst4CI ACNGT 1 cut(s) 351
Bst6I CTCTTC 1 cut(s) 228
BstAPI GCANNNNNTGC 2 cut(s) 151, 449
BstC8I GCNNGC 2 cut(s) 6, 321
BstDEI CTNAG 6 cut(s) 35, 67, 122, 130, 155, 454
BstEII GGTNACC 1 cut(s) 134
BstFNI CGCG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 1 cut(s) 103
BstKTI GATC 5 cut(s) 185, 240, 286, 381, 414
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 5 cut(s) 182, 237, 283, 378, 411
BstMWI GCNNNNNNNGC 5 cut(s) 47, 100, 151, 320, 449
BstNSI RCATGY 1 cut(s) 149
BstPI GGTNACC 1 cut(s) 134
BstSFI CTRYAG 1 cut(s) 309
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 3 cut(s) 87, 265, 323
BstX2I RGATCY 2 cut(s) 182, 237
BstYI RGATCY 2 cut(s) 182, 237
Bsu36I CCTNAGG 2 cut(s) 130, 454
BsuRI GGCC 6 cut(s) 41, 127, 212, 335, 345, 468
BtuMI TCGCGA 1 cut(s) 288
Cac8I GCNNGC 2 cut(s) 6, 321
CciI TCATGA 1 cut(s) 479
CfoI GCGC 1 cut(s) 103
Cfr13I GGNCC 4 cut(s) 39, 111, 126, 343
CseI GACGC 1 cut(s) 176
CviAII CATG 5 cut(s) 61, 146, 226, 262, 480
DdeI CTNAG 6 cut(s) 35, 67, 122, 130, 155, 454
DpnI GATC 5 cut(s) 184, 239, 285, 380, 413
DpnII GATC 5 cut(s) 182, 237, 283, 378, 411
EaeI YGGCCR 1 cut(s) 333
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Ecl136II GAGCTC 2 cut(s) 94, 367
Eco130I CCWWGG 2 cut(s) 115, 469
Eco147I AGGCCT 1 cut(s) 468
Eco24I GRGCYC 2 cut(s) 96, 369
Eco31I GGTCTC 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 111
Eco53kI GAGCTC 2 cut(s) 94, 367
Eco81I CCTNAGG 2 cut(s) 130, 454
Eco88I CYCGRG 1 cut(s) 95
Eco91I GGTNACC 1 cut(s) 134
EcoICRI GAGCTC 2 cut(s) 94, 367
EcoO109I RGGNCCY 1 cut(s) 126
EcoO65I GGTNACC 1 cut(s) 134
EcoT14I CCWWGG 2 cut(s) 115, 469
EcoT22I ATGCAT 1 cut(s) 147
EcoT38I GRGCYC 2 cut(s) 96, 369
ErhI CCWWGG 2 cut(s) 115, 469
FaeI CATG 5 cut(s) 64, 149, 229, 265, 483
FaiI YATR 8 cut(s) 62, 147, 227, 245, 263, 360, 437, 481
FaqI GGGAC 1 cut(s) 97
FatI CATG 5 cut(s) 60, 145, 225, 261, 479
FbaI TGATCA 2 cut(s) 378, 411
Fnu4HI GCNGC 3 cut(s) 76, 254, 312
FriOI GRGCYC 2 cut(s) 96, 369
Fsp4HI GCNGC 3 cut(s) 76, 254, 312
FspBI CTAG 3 cut(s) 89, 116, 464
GlaI GCGC 1 cut(s) 102
GluI GCNGC 3 cut(s) 76, 254, 312
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 6 cut(s) 41, 127, 212, 335, 345, 468
HapII CCGG 1 cut(s) 109
HgaI GACGC 1 cut(s) 176
HhaI GCGC 1 cut(s) 103
Hin1II CATG 5 cut(s) 64, 149, 229, 265, 483
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HinfI GANTC 3 cut(s) 54, 65, 120
HpaII CCGG 1 cut(s) 109
HphI GGTGA 2 cut(s) 73, 146
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 3 cut(s) 89, 287, 480
Hpy99I CGWCG 1 cut(s) 293
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4V TGCA 4 cut(s) 8, 145, 311, 431
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 100, 151, 320, 449
HpyF3I CTNAG 6 cut(s) 35, 67, 122, 130, 155, 454
Hsp92II CATG 5 cut(s) 64, 149, 229, 265, 483
HspAI GCGC 1 cut(s) 101
Ksp22I TGATCA 2 cut(s) 378, 411
Kzo9I GATC 5 cut(s) 182, 237, 283, 378, 411
LmnI GCTCC 1 cut(s) 364
LpnPI CCDG 8 cut(s) 18, 109, 117, 122, 234, 263, 305, 441
Lsp1109I GCAGC 3 cut(s) 87, 265, 323
MaeI CTAG 3 cut(s) 89, 116, 464
MaeIII GTNAC 1 cut(s) 134
MalI GATC 5 cut(s) 184, 239, 285, 380, 413
MboI GATC 5 cut(s) 182, 237, 283, 378, 411
MboII GAAGA 2 cut(s) 37, 245
MflI RGATCY 2 cut(s) 182, 237
MhlI GDGCHC 2 cut(s) 96, 369
MlsI TGGCCA 1 cut(s) 335
MluCI AATT 2 cut(s) 191, 425
MluNI TGGCCA 1 cut(s) 335
MlyI GAGTC 2 cut(s) 59, 114
MnlI CCTC 5 cut(s) 89, 139, 259, 379, 463
Mox20I TGGCCA 1 cut(s) 335
Mph1103I ATGCAT 1 cut(s) 147
MscI TGGCCA 1 cut(s) 335
MseI TTAA 1 cut(s) 420
MslI CAYNNNNRTG 1 cut(s) 374
Msp20I TGGCCA 1 cut(s) 335
MspI CCGG 1 cut(s) 109
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 5 cut(s) 47, 100, 151, 320, 449
NdeII GATC 5 cut(s) 182, 237, 283, 378, 411
NlaIII CATG 5 cut(s) 64, 149, 229, 265, 483
NlaIV GGNNCC 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 134
NruI TCGCGA 1 cut(s) 288
NsiI ATGCAT 1 cut(s) 147
NspI RCATGY 1 cut(s) 149
PagI TCATGA 1 cut(s) 479
PceI AGGCCT 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 1 cut(s) 54
PkrI GCNGC 3 cut(s) 77, 255, 313
PleI GAGTC 2 cut(s) 59, 114
PpsI GAGTC 2 cut(s) 59, 114
PsiI TTATAA 1 cut(s) 245
Psp124BI GAGCTC 2 cut(s) 96, 369
PspEI GGTNACC 1 cut(s) 134
PspN4I GGNNCC 1 cut(s) 113
PspPI GGNCC 4 cut(s) 39, 111, 126, 343
PstI CTGCAG 1 cut(s) 313
PsuI RGATCY 2 cut(s) 182, 237
RruI TCGCGA 1 cut(s) 288
RseI CAYNNNNRTG 1 cut(s) 374
SacI GAGCTC 2 cut(s) 96, 369
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 3 cut(s) 76, 254, 312
Sau3AI GATC 5 cut(s) 182, 237, 283, 378, 411
Sau96I GGNCC 4 cut(s) 39, 111, 126, 343
SchI GAGTC 2 cut(s) 59, 114
SduI GDGCHC 2 cut(s) 96, 369
SetI ASST 7 cut(s) 96, 136, 270, 321, 369, 419, 460
SfcI CTRYAG 1 cut(s) 309
SinI GGWCC 1 cut(s) 111
SmiMI CAYNNNNRTG 1 cut(s) 374
Sse9I AATT 2 cut(s) 191, 425
SseBI AGGCCT 1 cut(s) 468
SspMI CTAG 3 cut(s) 89, 116, 464
SstI GAGCTC 2 cut(s) 96, 369
StuI AGGCCT 1 cut(s) 468
StyI CCWWGG 2 cut(s) 115, 469
TaaI ACNGT 1 cut(s) 351
TaqI TCGA 1 cut(s) 57
TasI AATT 2 cut(s) 191, 425
TfiI GAWTC 1 cut(s) 54
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 3 cut(s) 75, 253, 311
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 3 cut(s) 242, 246, 438
VpaK11BI GGWCC 1 cut(s) 111
XapI RAATTY 2 cut(s) 191, 425
XbaI TCTAGA 1 cut(s) 88
XceI RCATGY 1 cut(s) 149
XmaJI CCTAGG 1 cut(s) 115
XspI CTAG 3 cut(s) 89, 116, 464
Zsp2I ATGCAT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.