FvH4_4g28451

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005153
Physical Location & Seq
Reverse (-)
29105044 .. 29105683
640 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g28451.t1

Sequence Viewer

Length: 477 bp
ATGATGGCGAAGCTCGAGACCGACCAGCTCAAACAGCTCAAAGACATCTTCATGAGGTTTGACATGGACTCCGACGGCAGCCTAACCCACCTGGAGCTCGCCGCGCTGCTCCGGTCCCTCGGCGTCAAGCTCCACGTTCTGCTCAACAACATGGACGCCAATGGAAACGGCGCCGTGGAGTTCGAGGAGTTGGTGAACGCCATCATGCCGAACATGAACGACGAGATTCTGGTCAACCAAGAGCAACTCATGGAGGTCTTCCGGTCGTTCGACCGCGACGGTAACGGCTACATAACGGCGGCTGAGCTAGCGGGGTCGATGGCCAAGATGGGGCACCCCTTGACGTACCGGGAGTTGTCTGATATGATGAGGGAGGCAGACACCAACGGTGACGGCGTTATTAGCTTCAACGAGTTTGCCACCATCATGTCTCGATCCGCCGCTAACTTTCTCGAATTTCAAGTGTACATTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

159

Amino Acids

17.75

Weight (kDa)

4.45

Isoelectric Point (pI)

24.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 11 - 68 7.4e-09 EF-hand domain pair
EF-hand_7 PF13499 81 - 144 2.7e-16 EF-hand domain pair
EF-hand_6 PF13405 82 - 110 9.5e-07 EF-hand domain
EF-hand_1 PF00036 83 - 109 3.1e-06 EF hand domain
EF-hand_8 PF13833 98 - 143 1.6e-08 EF-hand domain pair
EF-hand_1 PF00036 118 - 145 1.3e-08 EF hand domain
EF-hand_5 PF13202 121 - 143 6.5e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 170, 333
AccII CGCG 2 cut(s) 104, 276
AciI CCGC 6 cut(s) 102, 274, 299, 311, 438, 441
AclWI GGATC 1 cut(s) 429
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 1 cut(s) 455
AcyI GRCGYC 3 cut(s) 123, 156, 171
AfaI GTAC 2 cut(s) 347, 467
AfiI CCNNNNNNNGG 1 cut(s) 330
AgsI TTSAA 2 cut(s) 409, 461
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 7 cut(s) 13, 28, 37, 97, 130, 307, 405
AluI AGCT 7 cut(s) 13, 28, 37, 97, 130, 307, 405
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 2 cut(s) 11, 435
AlwI GGATC 1 cut(s) 429
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 1 cut(s) 321
ApeKI GCWGC 2 cut(s) 78, 106
ApoI RAATTY 1 cut(s) 455
AspLEI GCGC 2 cut(s) 106, 173
AspS9I GGNCC 1 cut(s) 114
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 2 cut(s) 205, 401
AsuNHI GCTAGC 1 cut(s) 307
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 1 cut(s) 114
BaeGI GKGCMC 1 cut(s) 336
BalI TGGCCA 1 cut(s) 323
BanI GGYRCC 2 cut(s) 170, 333
BanII GRGCYC 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 90, 93
BccI CCATC 4 cut(s) 209, 313, 322, 431
BceAI ACGGC 6 cut(s) 91, 158, 184, 301, 312, 409
BciT130I CCWGG 1 cut(s) 92
BcnI CCSGG 1 cut(s) 350
BcoDI GTCTC 2 cut(s) 11, 435
BfaI CTAG 1 cut(s) 308
BfoI RGCGCY 1 cut(s) 174
BisI GCNGC 5 cut(s) 79, 102, 107, 300, 441
BlpI GCTNAGC 1 cut(s) 303
BlsI GCNGC 5 cut(s) 80, 103, 108, 301, 442
Bme1390I CCNGG 2 cut(s) 92, 350
Bme18I GGWCC 1 cut(s) 114
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 3 cut(s) 116, 172, 335
BmrFI CCNGG 2 cut(s) 92, 350
BmtI GCTAGC 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 113
Bpu1102I GCTNAGC 1 cut(s) 303
BpuMI CCSGG 1 cut(s) 350
BsaHI GRCGYC 3 cut(s) 123, 156, 171
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 118, 174
BsaWI WCCGGW 2 cut(s) 111, 261
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bsc4I CCNNNNNNNGG 1 cut(s) 330
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 2 cut(s) 118, 174
BseLI CCNNNNNNNGG 1 cut(s) 330
BseMII CTCAG 1 cut(s) 294
BseRI GAGGAG 1 cut(s) 200
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 2 cut(s) 90, 93
Bsh1236I CGCG 2 cut(s) 104, 276
Bsh1285I CGRYCG 2 cut(s) 266, 274
BshFI GGCC 1 cut(s) 323
BshNI GGYRCC 2 cut(s) 170, 333
BsiEI CGRYCG 2 cut(s) 266, 274
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 3 cut(s) 112, 262, 349
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 330
BsmAI GTCTC 2 cut(s) 11, 435
BsmFI GGGAC 1 cut(s) 100
BsnI GGCC 1 cut(s) 323
Bso31I GGTCTC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 99, 336
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 1 cut(s) 434
Bsp1720I GCTNAGC 1 cut(s) 303
BspACI CCGC 6 cut(s) 102, 274, 299, 311, 438, 441
BspANI GGCC 1 cut(s) 323
BspCNI CTCAG 1 cut(s) 295
BspFNI CGCG 2 cut(s) 104, 276
BspHI TCATGA 1 cut(s) 51
BspLI GGNNCC 3 cut(s) 116, 172, 335
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 1 cut(s) 429
BspT107I GGYRCC 2 cut(s) 170, 333
BspTNI GGTCTC 1 cut(s) 11
BsrGI TGTACA 1 cut(s) 465
BssECI CCNNGG 2 cut(s) 118, 174
BssMI GATC 1 cut(s) 434
BssNI GRCGYC 3 cut(s) 123, 156, 171
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 2 cut(s) 281, 389
BstACI GRCGYC 3 cut(s) 123, 156, 171
BstAUI TGTACA 1 cut(s) 465
BstC8I GCNNGC 2 cut(s) 99, 309
BstDEI CTNAG 1 cut(s) 303
BstDSI CCRYGG 1 cut(s) 174
BstFNI CGCG 2 cut(s) 104, 276
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 2 cut(s) 106, 173
BstKTI GATC 1 cut(s) 437
BstMAI GTCTC 2 cut(s) 11, 435
BstMBI GATC 1 cut(s) 434
BstMCI CGRYCG 2 cut(s) 266, 274
BstMWI GCNNNNNNNGC 4 cut(s) 34, 103, 308, 402
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 2 cut(s) 90, 348
BstSLI GKGCMC 1 cut(s) 336
BstUI CGCG 2 cut(s) 104, 276
BstV1I GCAGC 2 cut(s) 90, 93
BstV2I GAAGAC 1 cut(s) 250
BsuRI GGCC 1 cut(s) 323
BtgI CCRYGG 1 cut(s) 174
Cac8I GCNNGC 2 cut(s) 99, 309
CciI TCATGA 1 cut(s) 51
CfoI GCGC 2 cut(s) 106, 173
Cfr13I GGNCC 1 cut(s) 114
CseI GACGC 2 cut(s) 112, 164
Csp6I GTAC 2 cut(s) 346, 466
CviAII CATG 7 cut(s) 52, 64, 151, 205, 214, 250, 427
CviQI GTAC 2 cut(s) 346, 466
DdeI CTNAG 1 cut(s) 303
DinI GGCGCC 1 cut(s) 172
DpnI GATC 1 cut(s) 436
DpnII GATC 1 cut(s) 434
EaeI YGGCCR 1 cut(s) 321
EciI GGCGGA 1 cut(s) 427
Ecl136II GAGCTC 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 114
Eco53kI GAGCTC 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 14
EcoICRI GAGCTC 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 99
EgeI GGCGCC 1 cut(s) 172
EheI GGCGCC 1 cut(s) 172
FaeI CATG 7 cut(s) 55, 67, 154, 208, 217, 253, 430
FaiI YATR 9 cut(s) 53, 65, 152, 206, 215, 251, 293, 365, 428
FaqI GGGAC 1 cut(s) 100
FatI CATG 7 cut(s) 51, 63, 150, 204, 213, 249, 426
FauI CCCGC 1 cut(s) 304
Fnu4HI GCNGC 5 cut(s) 79, 102, 107, 300, 441
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 5 cut(s) 79, 102, 107, 300, 441
FspBI CTAG 1 cut(s) 308
GlaI GCGC 2 cut(s) 105, 172
GluI GCNGC 5 cut(s) 79, 102, 107, 300, 441
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 1 cut(s) 323
HapII CCGG 3 cut(s) 112, 262, 349
HgaI GACGC 2 cut(s) 112, 164
HhaI GCGC 2 cut(s) 106, 173
Hin1I GRCGYC 3 cut(s) 123, 156, 171
Hin1II CATG 7 cut(s) 55, 67, 154, 208, 217, 253, 430
Hin6I GCGC 2 cut(s) 104, 171
HinP1I GCGC 2 cut(s) 104, 171
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 2 cut(s) 68, 226
HpaII CCGG 3 cut(s) 112, 262, 349
HphI GGTGA 2 cut(s) 205, 401
Hpy166II GTNNAC 3 cut(s) 196, 235, 466
Hpy188I TCNGA 2 cut(s) 73, 361
Hpy188III TCNNGA 4 cut(s) 16, 52, 432, 452
Hpy8I GTNNAC 3 cut(s) 196, 235, 466
Hpy99I CGWCG 3 cut(s) 77, 224, 281
HpyCH4III ACNGT 2 cut(s) 281, 389
HpyCH4IV ACGT 2 cut(s) 135, 344
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 103, 308, 402
HpyF3I CTNAG 1 cut(s) 303
HpySE526I ACGT 2 cut(s) 135, 344
Hsp92I GRCGYC 3 cut(s) 123, 156, 171
Hsp92II CATG 7 cut(s) 55, 67, 154, 208, 217, 253, 430
HspAI GCGC 2 cut(s) 104, 171
KasI GGCGCC 1 cut(s) 170
Kzo9I GATC 1 cut(s) 434
LmnI GCTCC 3 cut(s) 94, 114, 135
LpnPI CCDG 7 cut(s) 38, 77, 104, 125, 215, 275, 362
Lsp1109I GCAGC 2 cut(s) 90, 93
MaeI CTAG 1 cut(s) 308
MaeII ACGT 2 cut(s) 135, 344
MaeIII GTNAC 2 cut(s) 281, 389
MalI GATC 1 cut(s) 436
MboI GATC 1 cut(s) 434
MboII GAAGA 2 cut(s) 40, 250
MhlI GDGCHC 2 cut(s) 99, 336
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 1 cut(s) 455
MluNI TGGCCA 1 cut(s) 323
Mly113I GGCGCC 1 cut(s) 171
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 6 cut(s) 48, 128, 178, 247, 363, 367
Mox20I TGGCCA 1 cut(s) 323
MscI TGGCCA 1 cut(s) 323
MslI CAYNNNNRTG 2 cut(s) 50, 425
Msp20I TGGCCA 1 cut(s) 323
MspI CCGG 3 cut(s) 112, 262, 349
MspR9I CCNGG 2 cut(s) 92, 350
MvaI CCWGG 1 cut(s) 92
MvnI CGCG 2 cut(s) 104, 276
MwoI GCNNNNNNNGC 4 cut(s) 34, 103, 308, 402
NarI GGCGCC 1 cut(s) 171
NciI CCSGG 1 cut(s) 350
NdeII GATC 1 cut(s) 434
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 7 cut(s) 55, 67, 154, 208, 217, 253, 430
NlaIV GGNNCC 3 cut(s) 116, 172, 335
NmeAIII GCCGAG 1 cut(s) 99
NmuCI GTSAC 1 cut(s) 389
PaeR7I CTCGAG 1 cut(s) 14
PagI TCATGA 1 cut(s) 51
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 5 cut(s) 80, 103, 108, 301, 442
PleI GAGTC 1 cut(s) 62
PluTI GGCGCC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 3 cut(s) 116, 172, 335
PspPI GGNCC 1 cut(s) 114
RsaI GTAC 2 cut(s) 347, 467
RsaNI GTAC 2 cut(s) 346, 466
RseI CAYNNNNRTG 2 cut(s) 50, 425
SacI GAGCTC 1 cut(s) 99
SatI GCNGC 5 cut(s) 79, 102, 107, 300, 441
Sau3AI GATC 1 cut(s) 434
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 92, 350
SduI GDGCHC 2 cut(s) 99, 336
SfoI GGCGCC 1 cut(s) 172
Sfr274I CTCGAG 1 cut(s) 14
SinI GGWCC 1 cut(s) 114
SlaI CTCGAG 1 cut(s) 14
SmiMI CAYNNNNRTG 2 cut(s) 50, 425
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
Sse9I AATT 1 cut(s) 455
SsiI CCGC 6 cut(s) 102, 274, 299, 311, 438, 441
SspDI GGCGCC 1 cut(s) 170
SspMI CTAG 1 cut(s) 308
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 90, 348
TaaI ACNGT 2 cut(s) 281, 389
TaiI ACGT 2 cut(s) 138, 347
TaqI TCGA 6 cut(s) 15, 183, 270, 317, 433, 453
TaqII GACCGA 1 cut(s) 35
TasI AATT 1 cut(s) 455
TatI WGTACW 1 cut(s) 465
TauI GCSGC 3 cut(s) 104, 302, 443
TfiI GAWTC 1 cut(s) 226
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 2 cut(s) 78, 106
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 2 cut(s) 40, 230
VpaK11BI GGWCC 1 cut(s) 114
XapI RAATTY 1 cut(s) 455
XhoI CTCGAG 1 cut(s) 14
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.