MD16G1051200.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005153
Physical Location & Seq
Reverse (-)
3663933 .. 3664409
477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1051200.v1.1.491

Sequence Viewer

Length: 477 bp
ATGATGGCCGCGCTCGGATCCGATCAGCTGAAGCAGCTGAAGGACATCTTCATGCGGTTCGACATGGACTCCGACGGGAGCCTCACCCAGCTCGAACTCGCCGCCCTCCTCCGCTCCCTCGGCCTCAAGCCCACCGGCGACCAGCTCCACGTCCTCCTCTCCAACATCGACGCCAACGGCAACGGCGCCATTGAGTTCGACGAGCTCGTCACCGCCATCCTCCCCGACATGAACGAGGAGATTTTGATCAACCAGGAGCAGCTCATGGAGGTCTTCCGATCCTTCGATCGCGACGGCAACGGCTACATCACCGCCGCCGAGCTCGCCGGTTCCATGGCCAAAATGGGCCACCCCTTGACGTACAGGGAGCTCTCCGATATGATGATGGAGGCCGACACGAACGGCGACGGCGTCATTAGTTTCAACGAATTCGCGACGATCATGTCTCGGTCCGCCGCTGATTTTCTTGGGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

159

Amino Acids

17.32

Weight (kDa)

4.16

Isoelectric Point (pI)

30.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 11 - 73 2.9e-11 EF-hand domain pair
EF-hand_6 PF13405 12 - 41 9e-06 EF-hand domain
EF-hand_7 PF13499 86 - 149 4.7e-15 EF-hand domain pair
EF-hand_6 PF13405 87 - 115 9.5e-07 EF-hand domain
EF-hand_1 PF00036 88 - 114 3.1e-06 EF hand domain
EF-hand_8 PF13833 103 - 148 4.2e-07 EF-hand domain pair
EF-hand_1 PF00036 123 - 150 4.4e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 206, 442
AccB1I GGYRCC 1 cut(s) 185
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 3 cut(s) 11, 291, 434
AciI CCGC 9 cut(s) 9, 55, 102, 112, 213, 312, 315, 453, 456
AclWI GGATC 3 cut(s) 12, 25, 273
AcoI YGGCCR 2 cut(s) 6, 336
AcsI RAATTY 1 cut(s) 428
AcuI CTGAAG 2 cut(s) 50, 59
AcyI GRCGYC 3 cut(s) 171, 186, 411
AfaI GTAC 1 cut(s) 362
AgsI TTSAA 1 cut(s) 424
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 8 cut(s) 28, 37, 91, 145, 205, 262, 322, 370
AluI AGCT 8 cut(s) 28, 37, 91, 145, 205, 262, 322, 370
Alw21I GWGCWC 3 cut(s) 207, 324, 372
Alw26I GTCTC 1 cut(s) 450
AlwI GGATC 3 cut(s) 12, 25, 273
AoxI GGCC 5 cut(s) 6, 121, 336, 346, 390
ApeKI GCWGC 2 cut(s) 34, 259
ApoI RAATTY 1 cut(s) 428
AspLEI GCGC 2 cut(s) 13, 188
AspS9I GGNCC 2 cut(s) 346, 450
AsuHPI GGTGA 3 cut(s) 76, 202, 301
AvaII GGWCC 1 cut(s) 450
BalI TGGCCA 1 cut(s) 338
BamHI GGATCC 1 cut(s) 17
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 4 cut(s) 207, 324, 372, 474
BbsI GAAGAC 1 cut(s) 265
Bbv12I GWGCWC 3 cut(s) 207, 324, 372
BbvI GCAGC 2 cut(s) 46, 271
BccI CCATC 2 cut(s) 224, 379
BceAI ACGGC 6 cut(s) 193, 199, 310, 316, 418, 424
BciT130I CCWGG 1 cut(s) 254
BclI TGATCA 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 450
BfoI RGCGCY 1 cut(s) 189
BisI GCNGC 6 cut(s) 9, 35, 102, 260, 315, 456
BlsI GCNGC 6 cut(s) 10, 36, 103, 261, 316, 457
Bme1390I CCNGG 1 cut(s) 254
Bme18I GGWCC 1 cut(s) 450
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 346, 450
BmiI GGNNCC 4 cut(s) 19, 80, 187, 331
BmrFI CCNGG 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 265
BpuEI CTTGAG 1 cut(s) 110
BsaBI GATNNNNATC 1 cut(s) 245
BsaHI GRCGYC 3 cut(s) 171, 186, 411
BsaJI CCNNGG 2 cut(s) 118, 333
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bse118I RCCGGY 2 cut(s) 134, 326
Bse8I GATNNNNATC 1 cut(s) 245
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 2 cut(s) 118, 333
BseGI GGATG 1 cut(s) 216
BseJI GATNNNNATC 1 cut(s) 245
BseRI GAGGAG 3 cut(s) 98, 146, 251
BseXI GCAGC 2 cut(s) 46, 271
BseYI CCCAGC 1 cut(s) 87
Bsh1236I CGCG 3 cut(s) 11, 291, 434
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 5 cut(s) 8, 123, 338, 348, 392
BshNI GGYRCC 1 cut(s) 185
BsiEI CGRYCG 1 cut(s) 289
BsiHKAI GWGCWC 3 cut(s) 207, 324, 372
BsiSI CCGG 2 cut(s) 135, 327
BsmAI GTCTC 1 cut(s) 450
BsnI GGCC 5 cut(s) 8, 123, 338, 348, 392
Bsp1286I GDGCHC 4 cut(s) 207, 324, 372, 474
Bsp143I GATC 6 cut(s) 17, 22, 246, 278, 286, 438
Bsp19I CCATGG 1 cut(s) 333
Bsp68I TCGCGA 2 cut(s) 291, 434
BspACI CCGC 9 cut(s) 9, 55, 102, 112, 213, 312, 315, 453, 456
BspANI GGCC 5 cut(s) 8, 123, 338, 348, 392
BspFNI CGCG 3 cut(s) 11, 291, 434
BspLI GGNNCC 4 cut(s) 19, 80, 187, 331
BspPI GGATC 3 cut(s) 12, 25, 273
BspT107I GGYRCC 1 cut(s) 185
BsrBI CCGCTC 1 cut(s) 114
BsrFI RCCGGY 2 cut(s) 134, 326
BssAI RCCGGY 2 cut(s) 134, 326
BssECI CCNNGG 2 cut(s) 118, 333
BssMI GATC 6 cut(s) 17, 22, 246, 278, 286, 438
BssNI GRCGYC 3 cut(s) 171, 186, 411
BssT1I CCWWGG 1 cut(s) 333
Bst2UI CCWGG 1 cut(s) 254
BstACI GRCGYC 3 cut(s) 171, 186, 411
BstC8I GCNNGC 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 216
BstFNI CGCG 3 cut(s) 11, 291, 434
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 2 cut(s) 13, 188
BstKTI GATC 6 cut(s) 20, 25, 249, 281, 289, 441
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 6 cut(s) 17, 22, 246, 278, 286, 438
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 3 cut(s) 34, 120, 323
BstNI CCWGG 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 252
BstUI CGCG 3 cut(s) 11, 291, 434
BstV1I GCAGC 2 cut(s) 46, 271
BstV2I GAAGAC 1 cut(s) 265
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 5 cut(s) 8, 123, 338, 348, 392
BtgI CCRYGG 1 cut(s) 333
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 1 cut(s) 216
BtuMI TCGCGA 2 cut(s) 291, 434
Cac8I GCNNGC 1 cut(s) 324
CfoI GCGC 2 cut(s) 13, 188
Cfr10I RCCGGY 2 cut(s) 134, 326
Cfr13I GGNCC 2 cut(s) 346, 450
CpoI CGGWCCG 1 cut(s) 450
CseI GACGC 2 cut(s) 179, 400
Csp6I GTAC 1 cut(s) 361
CspI CGGWCCG 1 cut(s) 450
CviAII CATG 6 cut(s) 52, 64, 229, 265, 334, 442
CviQI GTAC 1 cut(s) 361
DinI GGCGCC 1 cut(s) 187
DpnI GATC 6 cut(s) 19, 24, 248, 280, 288, 440
DpnII GATC 6 cut(s) 17, 22, 246, 278, 286, 438
DrdI GACNNNNNNGTC 2 cut(s) 206, 442
DseDI GACNNNNNNGTC 2 cut(s) 206, 442
EaeI YGGCCR 2 cut(s) 6, 336
EciI GGCGGA 1 cut(s) 442
Ecl136II GAGCTC 3 cut(s) 205, 322, 370
Eco130I CCWWGG 1 cut(s) 333
Eco24I GRGCYC 4 cut(s) 207, 324, 372, 474
Eco47I GGWCC 1 cut(s) 450
Eco53kI GAGCTC 3 cut(s) 205, 322, 370
Eco57I CTGAAG 2 cut(s) 50, 59
EcoICRI GAGCTC 3 cut(s) 205, 322, 370
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 333
EcoT38I GRGCYC 4 cut(s) 207, 324, 372, 474
EgeI GGCGCC 1 cut(s) 187
EheI GGCGCC 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 6 cut(s) 55, 67, 232, 268, 337, 445
FaiI YATR 7 cut(s) 53, 65, 230, 266, 335, 380, 443
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FatI CATG 6 cut(s) 51, 63, 228, 264, 333, 441
FbaI TGATCA 1 cut(s) 246
Fnu4HI GCNGC 6 cut(s) 9, 35, 102, 260, 315, 456
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 4 cut(s) 207, 324, 372, 474
Fsp4HI GCNGC 6 cut(s) 9, 35, 102, 260, 315, 456
GlaI GCGC 2 cut(s) 12, 187
GluI GCNGC 6 cut(s) 9, 35, 102, 260, 315, 456
GsaI CCCAGC 1 cut(s) 91
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 5 cut(s) 8, 123, 338, 348, 392
HapII CCGG 2 cut(s) 135, 327
HgaI GACGC 2 cut(s) 179, 400
HhaI GCGC 2 cut(s) 13, 188
Hin1I GRCGYC 3 cut(s) 171, 186, 411
Hin1II CATG 6 cut(s) 55, 67, 232, 268, 337, 445
Hin6I GCGC 2 cut(s) 11, 186
HinP1I GCGC 2 cut(s) 11, 186
HinfI GANTC 1 cut(s) 68
HpaII CCGG 2 cut(s) 135, 327
HphI GGTGA 3 cut(s) 76, 202, 301
Hpy188I TCNGA 6 cut(s) 17, 22, 73, 278, 376, 476
Hpy188III TCNNGA 2 cut(s) 290, 433
Hpy99I CGWCG 6 cut(s) 77, 173, 203, 296, 410, 439
HpyAV CCTTC 2 cut(s) 34, 292
HpyCH4IV ACGT 2 cut(s) 150, 359
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 120, 323
HpySE526I ACGT 2 cut(s) 150, 359
Hsp92I GRCGYC 3 cut(s) 171, 186, 411
Hsp92II CATG 6 cut(s) 55, 67, 232, 268, 337, 445
HspAI GCGC 2 cut(s) 11, 186
KasI GGCGCC 1 cut(s) 185
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 6 cut(s) 17, 22, 246, 278, 286, 438
LmnI GCTCC 5 cut(s) 78, 119, 150, 256, 367
LpnPI CCDG 7 cut(s) 101, 148, 155, 239, 266, 340, 349
Lsp1109I GCAGC 2 cut(s) 46, 271
MaeII ACGT 2 cut(s) 150, 359
MaeIII GTNAC 1 cut(s) 208
MalI GATC 6 cut(s) 19, 24, 248, 280, 288, 440
MbiI CCGCTC 1 cut(s) 114
MboI GATC 6 cut(s) 17, 22, 246, 278, 286, 438
MboII GAAGA 2 cut(s) 40, 265
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 4 cut(s) 207, 324, 372, 474
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 338
Mly113I GGCGCC 1 cut(s) 186
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 2 cut(s) 96, 186
Mox20I TGGCCA 1 cut(s) 338
MscI TGGCCA 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 338
MspA1I CMGCKG 3 cut(s) 28, 37, 458
MspI CCGG 2 cut(s) 135, 327
MspR9I CCNGG 1 cut(s) 254
MvaI CCWGG 1 cut(s) 254
MvnI CGCG 3 cut(s) 11, 291, 434
MwoI GCNNNNNNNGC 3 cut(s) 34, 120, 323
NarI GGCGCC 1 cut(s) 186
NcoI CCATGG 1 cut(s) 333
NdeII GATC 6 cut(s) 17, 22, 246, 278, 286, 438
NlaIII CATG 6 cut(s) 55, 67, 232, 268, 337, 445
NlaIV GGNNCC 4 cut(s) 19, 80, 187, 331
NmeAIII GCCGAG 2 cut(s) 99, 343
NmuCI GTSAC 1 cut(s) 208
NruI TCGCGA 2 cut(s) 291, 434
PcsI WCGNNNNNNNCGW 2 cut(s) 204, 408
PflFI GACNNNGTC 1 cut(s) 410
PkrI GCNGC 6 cut(s) 10, 36, 103, 261, 316, 457
Ple19I CGATCG 1 cut(s) 289
PleI GAGTC 1 cut(s) 62
PluTI GGCGCC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 62
Psp124BI GAGCTC 3 cut(s) 207, 324, 372
Psp6I CCWGG 1 cut(s) 252
PspFI CCCAGC 1 cut(s) 87
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 4 cut(s) 19, 80, 187, 331
PspPI GGNCC 2 cut(s) 346, 450
PsuI RGATCY 1 cut(s) 17
PsyI GACNNNGTC 1 cut(s) 410
PvuI CGATCG 1 cut(s) 289
PvuII CAGCTG 2 cut(s) 28, 37
RruI TCGCGA 2 cut(s) 291, 434
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 1 cut(s) 50
Rsr2I CGGWCCG 1 cut(s) 450
RsrII CGGWCCG 1 cut(s) 450
SacI GAGCTC 3 cut(s) 207, 324, 372
SatI GCNGC 6 cut(s) 9, 35, 102, 260, 315, 456
Sau3AI GATC 6 cut(s) 17, 22, 246, 278, 286, 438
Sau96I GGNCC 2 cut(s) 346, 450
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 254
SduI GDGCHC 4 cut(s) 207, 324, 372, 474
SfoI GGCGCC 1 cut(s) 187
SgrAI CRCCGGYG 1 cut(s) 134
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 1 cut(s) 428
SsiI CCGC 9 cut(s) 9, 55, 102, 112, 213, 312, 315, 453, 456
SspDI GGCGCC 1 cut(s) 185
SstI GAGCTC 3 cut(s) 207, 324, 372
StyD4I CCNGG 1 cut(s) 252
StyI CCWWGG 1 cut(s) 333
TaiI ACGT 2 cut(s) 153, 362
TaqI TCGA 5 cut(s) 60, 93, 168, 198, 285
TaqII GACCGA 1 cut(s) 438
TasI AATT 1 cut(s) 428
TauI GCSGC 4 cut(s) 11, 104, 317, 458
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 2 cut(s) 34, 259
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 40, 245
Tth111I GACNNNGTC 1 cut(s) 410
VpaK11BI GGWCC 1 cut(s) 450
XapI RAATTY 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.