AT3G25940

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008445
Physical Location & Seq
Forward (+)
9494626 .. 9496092
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25940.1

Sequence Viewer

Length: 360 bp
ATGGAGAAATCTAGAGAAAGCGAGTTCTTGTTCTGTAATTTGTGTGGGACTATGCTTGTCTTGAAATCAACCAAGTATGCAGAATGTCCACATTGCAAAACAACACGGAATGCAAAAGATATCATCGACAAGGAAATAGCTTACACAGTTTCTGCTGAGGATATCAGAAGAGAACTAGGAATATCATTGTTTGGTGAAAAAACGCAGGCAGAAGCTGAGCTACCAAAGATCAAAAAGGCATGCGAGAAATGCCAGCATCCTGAGCTTGTATACACAACCAGACAGACGAGATCAGCTGATGAAGGACAGACAACATATTACACTTGCCCCAATTGTGCTCATAGATTCACAGAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020
Pfam Domains
Protein Families
TFIIS_C

Protein Analysis

119

Amino Acids

13.58

Weight (kDa)

6.81

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_TFIIS PF01096 80 - 117 2.6e-16 Transcription factor S-II (TFIIS)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 270
AgsI TTSAA 1 cut(s) 64
AluBI AGCT 5 cut(s) 140, 215, 220, 265, 296
AluI AGCT 5 cut(s) 140, 215, 220, 265, 296
Alw21I GWGCWC 1 cut(s) 340
AlwNI CAGNNNCTG 2 cut(s) 152, 215
AsuHPI GGTGA 1 cut(s) 206
BarI GAAGNNNNNNTAC 2 cut(s) 204, 236
Bbv12I GWGCWC 1 cut(s) 340
BbvCI CCTCAGC 1 cut(s) 156
BfaI CTAG 2 cut(s) 12, 176
BlpI GCTNAGC 1 cut(s) 216
BmsI GCATC 1 cut(s) 265
Bpu10I CCTNAGC 2 cut(s) 156, 261
Bpu1102I GCTNAGC 1 cut(s) 216
Bse3DI GCAATG 1 cut(s) 91
BseGI GGATG 1 cut(s) 256
BseMI GCAATG 1 cut(s) 91
BseMII CTCAG 3 cut(s) 147, 207, 252
BsiHKAI GWGCWC 1 cut(s) 340
BslFI GGGAC 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 61
BsmI GAATGC 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 340
Bsp143I GATC 2 cut(s) 228, 290
Bsp1720I GCTNAGC 1 cut(s) 216
BspCNI CTCAG 3 cut(s) 148, 208, 253
BsrDI GCAATG 1 cut(s) 91
BssMI GATC 2 cut(s) 228, 290
BssNAI GTATAC 1 cut(s) 271
Bst1107I GTATAC 1 cut(s) 271
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 1 cut(s) 163
BstC8I GCNNGC 3 cut(s) 207, 241, 254
BstDEI CTNAG 3 cut(s) 156, 216, 261
BstF5I GGATG 1 cut(s) 256
BstKTI GATC 2 cut(s) 231, 293
BstMBI GATC 2 cut(s) 228, 290
BstMWI GCNNNNNNNGC 2 cut(s) 249, 262
BstNSI RCATGY 1 cut(s) 243
BstZ17I GTATAC 1 cut(s) 271
BtsCI GGATG 1 cut(s) 256
Cac8I GCNNGC 3 cut(s) 207, 241, 254
CaiI CAGNNNCTG 2 cut(s) 152, 215
CviAII CATG 1 cut(s) 240
CviJI RGCY 5 cut(s) 140, 215, 220, 265, 296
CviKI_1 RGCY 5 cut(s) 140, 215, 220, 265, 296
DdeI CTNAG 3 cut(s) 156, 216, 261
DpnI GATC 2 cut(s) 230, 292
DpnII GATC 2 cut(s) 228, 290
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco32I GATATC 2 cut(s) 121, 163
EcoRV GATATC 2 cut(s) 121, 163
FaeI CATG 1 cut(s) 243
FaiI YATR 6 cut(s) 53, 78, 241, 271, 316, 342
FaqI GGGAC 1 cut(s) 61
FatI CATG 1 cut(s) 239
FblI GTMKAC 1 cut(s) 270
FokI GGATG 1 cut(s) 243
FspBI CTAG 2 cut(s) 12, 176
Hin1II CATG 1 cut(s) 243
HinfI GANTC 1 cut(s) 345
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 2 cut(s) 89, 271
Hpy188I TCNGA 1 cut(s) 167
Hpy188III TCNNGA 3 cut(s) 12, 61, 260
Hpy8I GTNNAC 2 cut(s) 89, 271
HpyAV CCTTC 2 cut(s) 296, 347
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4V TGCA 3 cut(s) 80, 96, 113
HpyF10VI GCNNNNNNNGC 2 cut(s) 249, 262
HpyF3I CTNAG 3 cut(s) 156, 216, 261
Hsp92II CATG 1 cut(s) 243
Kzo9I GATC 2 cut(s) 228, 290
LpnPI CCDG 4 cut(s) 191, 266, 273, 292
LweI GCATC 1 cut(s) 265
MaeI CTAG 2 cut(s) 12, 176
MalI GATC 2 cut(s) 230, 292
MboI GATC 2 cut(s) 228, 290
MboII GAAGA 1 cut(s) 180
MfeI CAATTG 1 cut(s) 331
MhlI GDGCHC 1 cut(s) 340
MluCI AATT 2 cut(s) 37, 331
MnlI CCTC 1 cut(s) 151
MspA1I CMGCKG 1 cut(s) 296
MunI CAATTG 1 cut(s) 331
Mva1269I GAATGC 1 cut(s) 115
MwoI GCNNNNNNNGC 2 cut(s) 249, 262
NdeII GATC 2 cut(s) 228, 290
NlaIII CATG 1 cut(s) 243
NspI RCATGY 1 cut(s) 243
PaeI GCATGC 1 cut(s) 243
PctI GAATGC 1 cut(s) 115
PfeI GAWTC 1 cut(s) 345
PstNI CAGNNNCTG 2 cut(s) 152, 215
PvuII CAGCTG 1 cut(s) 296
Sau3AI GATC 2 cut(s) 228, 290
SduI GDGCHC 1 cut(s) 340
SetI ASST 6 cut(s) 142, 217, 222, 267, 298, 358
SfaNI GCATC 1 cut(s) 265
SphI GCATGC 1 cut(s) 243
Sse9I AATT 2 cut(s) 37, 331
SspMI CTAG 2 cut(s) 12, 176
TaaI ACNGT 1 cut(s) 148
TaqI TCGA 1 cut(s) 126
TasI AATT 2 cut(s) 37, 331
TfiI GAWTC 1 cut(s) 345
TspDTI ATGAA 1 cut(s) 315
TspGWI ACGGA 1 cut(s) 121
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 1 cut(s) 243
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 2 cut(s) 12, 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.