Rh2DG331300

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008445
Physical Location & Seq
Reverse (-)
42301364 .. 42302707
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG331300.1

Sequence Viewer

Length: 444 bp
ATGCTCCAGAGAATTCATGCAGCAAAATATCTAATGGATTTTACTTTTGTGAATGCCCCTATAGGTTTACTTTCAACAATGTCGTCCATGTCAGAGTCTCGTGCTTTCCTGTTCTGCAACAGCTGTGGGTGTATGCTATCTTTGACGACAACTCCTTATGCTAAACCTGGCCGATGCCCCTATTGCGACCATCCGCGACCTATAGAAGGGCTCTCCAAATGTAAATATTCGTACACAGTCAGTAAAGAGGATATTCAAAGGGAGCTAGGCAAATCAACAATTAAGGAGGAAAAGACTGAATTGGCAAAGACGGACATGAAAAAATGTGAAAAGTGTGGCCACAATGAGCATACATATTTTTCTAGACAGATGAGATCAGCTGATGAGGGGGCAACTACTTTCTACACTTGCACCAAGTGCAATAATAGTTTCAGTGAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020
Pfam Domains
Protein Families
TFIIS_C

Protein Analysis

147

Amino Acids

16.68

Weight (kDa)

8.5

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_TFIIS PF01096 107 - 144 4.3e-17 Transcription factor S-II (TFIIS)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 196
AciI CCGC 1 cut(s) 194
AcoI YGGCCR 2 cut(s) 169, 337
AcsI RAATTY 1 cut(s) 12
AdeI CACNNNGTG 1 cut(s) 417
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 206
AgsI TTSAA 2 cut(s) 75, 257
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 3 cut(s) 123, 265, 380
AluI AGCT 3 cut(s) 123, 265, 380
Alw26I GTCTC 1 cut(s) 102
AoxI GGCC 2 cut(s) 169, 337
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 1 cut(s) 12
BalI TGGCCA 1 cut(s) 339
BanII GRGCYC 1 cut(s) 213
BauI CACGAG 1 cut(s) 99
BbvI GCAGC 1 cut(s) 32
BccI CCATC 1 cut(s) 198
BciT130I CCWGG 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 102
BfaI CTAG 2 cut(s) 266, 363
BfmI CTRYAG 2 cut(s) 60, 201
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
Bme1390I CCNGG 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 164
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bsc4I CCNNNNNNNGG 1 cut(s) 206
BseBI CCWGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 32
Bsh1236I CGCG 1 cut(s) 196
BshFI GGCC 2 cut(s) 171, 339
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 102
BsmI GAATGC 1 cut(s) 58
BsnI GGCC 2 cut(s) 171, 339
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 1 cut(s) 374
BspACI CCGC 1 cut(s) 194
BspANI GGCC 2 cut(s) 171, 339
BspFNI CGCG 1 cut(s) 196
BssMI GATC 1 cut(s) 374
BssSI CACGAG 1 cut(s) 99
Bst2BI CACGAG 1 cut(s) 99
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 238
BstAPI GCANNNNNTGC 1 cut(s) 417
BstENI CCTNNNNNAGG 1 cut(s) 204
BstF5I GGATG 1 cut(s) 190
BstFNI CGCG 1 cut(s) 196
BstKTI GATC 1 cut(s) 377
BstMAI GTCTC 1 cut(s) 102
BstMBI GATC 1 cut(s) 374
BstMWI GCNNNNNNNGC 2 cut(s) 183, 417
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 2 cut(s) 60, 201
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 32
BsuRI GGCC 2 cut(s) 171, 339
BtsCI GGATG 1 cut(s) 190
BtsIMutI CAGTG 1 cut(s) 439
Csp6I GTAC 1 cut(s) 232
CspCI CAANNNNNGTGG 2 cut(s) 106, 141
CviAII CATG 3 cut(s) 17, 88, 316
CviJI RGCY 6 cut(s) 123, 171, 211, 265, 339, 380
CviKI_1 RGCY 6 cut(s) 123, 171, 211, 265, 339, 380
CviQI GTAC 1 cut(s) 232
DpnI GATC 1 cut(s) 376
DpnII GATC 1 cut(s) 374
DraIII CACNNNGTG 1 cut(s) 417
EaeI YGGCCR 2 cut(s) 169, 337
Eco24I GRGCYC 1 cut(s) 213
EcoNI CCTNNNNNAGG 1 cut(s) 204
EcoRI GAATTC 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 166
EcoT38I GRGCYC 1 cut(s) 213
FaeI CATG 3 cut(s) 20, 91, 319
FaiI YATR 9 cut(s) 18, 62, 89, 134, 159, 203, 317, 351, 355
FatI CATG 3 cut(s) 16, 87, 315
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 1 cut(s) 177
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 2 cut(s) 266, 363
GluI GCNGC 1 cut(s) 21
HaeIII GGCC 2 cut(s) 171, 339
Hin1II CATG 3 cut(s) 20, 91, 319
HinfI GANTC 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 68, 234
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 2 cut(s) 7, 363
Hpy8I GTNNAC 2 cut(s) 68, 234
HpyAV CCTTC 1 cut(s) 200
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4V TGCA 4 cut(s) 20, 117, 411, 420
HpyF10VI GCNNNNNNNGC 2 cut(s) 183, 417
Hsp92II CATG 3 cut(s) 20, 91, 319
Kzo9I GATC 1 cut(s) 374
LmnI GCTCC 2 cut(s) 9, 262
LpnPI CCDG 4 cut(s) 20, 122, 153, 180
Lsp1109I GCAGC 1 cut(s) 32
LweI GCATC 1 cut(s) 164
MaeI CTAG 2 cut(s) 266, 363
MalI GATC 1 cut(s) 376
MboI GATC 1 cut(s) 374
MhlI GDGCHC 1 cut(s) 213
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 4 cut(s) 12, 279, 299, 439
MluNI TGGCCA 1 cut(s) 339
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 3 cut(s) 241, 280, 379
Mox20I TGGCCA 1 cut(s) 339
MscI TGGCCA 1 cut(s) 339
MseI TTAA 2 cut(s) 282, 442
Msp20I TGGCCA 1 cut(s) 339
MspA1I CMGCKG 2 cut(s) 123, 380
MspR9I CCNGG 1 cut(s) 168
Mva1269I GAATGC 1 cut(s) 58
MvaI CCWGG 1 cut(s) 168
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 2 cut(s) 183, 417
NdeII GATC 1 cut(s) 374
NlaIII CATG 3 cut(s) 20, 91, 319
PctI GAATGC 1 cut(s) 58
PkrI GCNGC 1 cut(s) 22
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PvuII CAGCTG 2 cut(s) 123, 380
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
SaqAI TTAA 2 cut(s) 282, 442
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 1 cut(s) 374
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 213
SetI ASST 6 cut(s) 67, 125, 169, 202, 267, 382
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 2 cut(s) 60, 201
Sse9I AATT 4 cut(s) 12, 279, 299, 439
SsiI CCGC 1 cut(s) 194
SspI AATATT 1 cut(s) 227
SspMI CTAG 2 cut(s) 266, 363
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 1 cut(s) 238
TasI AATT 4 cut(s) 12, 279, 299, 439
Tru1I TTAA 2 cut(s) 282, 442
Tru9I TTAA 2 cut(s) 282, 442
TscAI CASTG 1 cut(s) 439
TseI GCWGC 1 cut(s) 20
TspDTI ATGAA 2 cut(s) 5, 332
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 1 cut(s) 439
XagI CCTNNNNNAGG 1 cut(s) 204
XapI RAATTY 1 cut(s) 12
XbaI TCTAGA 1 cut(s) 362
XspI CTAG 2 cut(s) 266, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.