MD08G1010500.v1.1

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008445
Physical Location & Seq
Forward (+)
774300 .. 776131
1832 bp
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UTR
Exon/CDS
Intron
MD08G1010500.v1.1.491

Sequence Viewer

Length: 354 bp
ATGGCATATTCCCAAGGTCGCAATTTCTTGTTCTGCGACTTGTGTGGGAGTATGCTAAATTTGAATTCAACTAAGTTTGCTCAATGCCCTTTGTGCAAGTTAAAGCGAAATGTGAAAGAGATATCCGGAAGAGAAATATCTTACAAAGTCACTGCTGAGGATATCAGAAGAGATCTAGGCATATCAATAATTCCTGAAGAGAAGGTGCAATTGCAAAAGACAGATGCTAAAACATGTGAAAAATGTGGCCACAACGAGCATACATATTACTCCCGACAGATGAGATCGGCAGATGAAGGGGCAACTACTTTCTATGTGTGCACCAATTGCCAGCACCAGTTTACTGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020
Pfam Domains
Protein Families
TFIIS_C

Protein Analysis

118

Amino Acids

13.43

Weight (kDa)

8.11

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_TFIIS PF01096 78 - 115 3.2e-17 Transcription factor S-II (TFIIS)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 125
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 2 cut(s) 58, 64
AcuI CTGAAG 1 cut(s) 216
AflIII ACRYGT 1 cut(s) 233
AgsI TTSAA 2 cut(s) 64, 69
Alw21I GWGCWC 1 cut(s) 323
Alw44I GTGCAC 1 cut(s) 319
Aor13HI TCCGGA 1 cut(s) 125
AoxI GGCC 1 cut(s) 247
ApaLI GTGCAC 1 cut(s) 319
ApoI RAATTY 2 cut(s) 58, 64
BaeGI GKGCMC 1 cut(s) 323
BalI TGGCCA 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 323
BbvCI CCTCAGC 1 cut(s) 156
BfaI CTAG 1 cut(s) 176
BglII AGATCT 1 cut(s) 172
BmsI GCATC 1 cut(s) 214
Bpu10I CCTNAGC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 13
BsaWI WCCGGW 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 337
BseAI TCCGGA 1 cut(s) 125
BseDI CCNNGG 1 cut(s) 13
BseMII CTCAG 2 cut(s) 147, 336
BseNI ACTGG 1 cut(s) 337
BseSI GKGCMC 1 cut(s) 323
BshFI GGCC 1 cut(s) 249
BsiHKAI GWGCWC 1 cut(s) 323
BsiSI CCGG 1 cut(s) 126
BsnI GGCC 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 323
Bsp13I TCCGGA 1 cut(s) 125
Bsp143I GATC 2 cut(s) 172, 284
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 2 cut(s) 148, 337
BspEI TCCGGA 1 cut(s) 125
BsrI ACTGG 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 13
BssMI GATC 2 cut(s) 172, 284
BssT1I CCWWGG 1 cut(s) 13
Bst6I CTCTTC 3 cut(s) 124, 163, 192
BstAPI GCANNNNNTGC 1 cut(s) 327
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 3 cut(s) 72, 156, 345
BstKTI GATC 2 cut(s) 175, 287
BstMBI GATC 2 cut(s) 172, 284
BstMWI GCNNNNNNNGC 2 cut(s) 93, 327
BstNSI RCATGY 1 cut(s) 237
BstSLI GKGCMC 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
BsuRI GGCC 1 cut(s) 249
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 1 cut(s) 150
Cac8I GCNNGC 1 cut(s) 332
CviAII CATG 1 cut(s) 234
CviJI RGCY 1 cut(s) 249
CviKI_1 RGCY 1 cut(s) 249
DdeI CTNAG 3 cut(s) 72, 156, 345
DpnI GATC 2 cut(s) 174, 286
DpnII GATC 2 cut(s) 172, 284
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 3 cut(s) 124, 163, 192
EarI CTCTTC 3 cut(s) 124, 163, 192
Eco130I CCWWGG 1 cut(s) 13
Eco32I GATATC 2 cut(s) 123, 163
Eco57I CTGAAG 1 cut(s) 216
EcoRI GAATTC 1 cut(s) 64
EcoRV GATATC 2 cut(s) 123, 163
EcoT14I CCWWGG 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 13
FaeI CATG 1 cut(s) 237
FaiI YATR 7 cut(s) 7, 53, 182, 235, 261, 265, 315
FatI CATG 1 cut(s) 233
FspBI CTAG 1 cut(s) 176
HaeIII GGCC 1 cut(s) 249
HapII CCGG 1 cut(s) 126
Hin1II CATG 1 cut(s) 237
HpaII CCGG 1 cut(s) 126
Hpy166II GTNNAC 2 cut(s) 321, 342
Hpy188I TCNGA 1 cut(s) 167
Hpy188III TCNNGA 3 cut(s) 126, 194, 273
Hpy8I GTNNAC 2 cut(s) 321, 342
HpyAV CCTTC 2 cut(s) 196, 290
HpyCH4V TGCA 4 cut(s) 96, 208, 214, 321
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 327
HpyF3I CTNAG 3 cut(s) 72, 156, 345
Hsp92II CATG 1 cut(s) 237
Kpn2I TCCGGA 1 cut(s) 125
Kzo9I GATC 2 cut(s) 172, 284
LpnPI CCDG 4 cut(s) 139, 207, 344, 350
LweI GCATC 1 cut(s) 214
MaeI CTAG 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 148
MalI GATC 2 cut(s) 174, 286
MboI GATC 2 cut(s) 172, 284
MboII GAAGA 3 cut(s) 141, 180, 209
MfeI CAATTG 2 cut(s) 209, 325
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 1 cut(s) 323
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 7 cut(s) 22, 58, 64, 189, 209, 325, 349
MluNI TGGCCA 1 cut(s) 249
MnlI CCTC 1 cut(s) 151
Mox20I TGGCCA 1 cut(s) 249
MroI TCCGGA 1 cut(s) 125
MscI TGGCCA 1 cut(s) 249
MseI TTAA 1 cut(s) 101
Msp20I TGGCCA 1 cut(s) 249
MspI CCGG 1 cut(s) 126
MunI CAATTG 2 cut(s) 209, 325
MwoI GCNNNNNNNGC 2 cut(s) 93, 327
NdeII GATC 2 cut(s) 172, 284
NlaIII CATG 1 cut(s) 237
NmuCI GTSAC 1 cut(s) 148
NspI RCATGY 1 cut(s) 237
PciI ACATGT 1 cut(s) 233
PscI ACATGT 1 cut(s) 233
PsuI RGATCY 1 cut(s) 172
SaqAI TTAA 1 cut(s) 101
Sau3AI GATC 2 cut(s) 172, 284
SduI GDGCHC 1 cut(s) 323
SetI ASST 2 cut(s) 19, 207
SfaNI GCATC 1 cut(s) 214
Sse9I AATT 7 cut(s) 22, 58, 64, 189, 209, 325, 349
SspMI CTAG 1 cut(s) 176
StyI CCWWGG 1 cut(s) 13
TasI AATT 7 cut(s) 22, 58, 64, 189, 209, 325, 349
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 309
TspRI CASTG 1 cut(s) 157
VneI GTGCAC 1 cut(s) 319
XapI RAATTY 2 cut(s) 58, 64
XceI RCATGY 1 cut(s) 237
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.