RLG00000018781

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008445
Physical Location & Seq
Reverse (-)
35989253 .. 35992252
3000 bp
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UTR
Exon/CDS
Intron
RLM00000018781

Sequence Viewer

Length: 630 bp
ATGTCTGAAGATACCTGGCCAAAAAATAATTGTAGAGGTTGCATTGATTTGTGTCCTGCTCCGTTTAGCCAACATTCTGATGATATCCTCATGATTGACCTCTCTGAGAACTTTCCATGGAGCAATTTATGCTTTGATTTTATCATTCATATTCACCTCATCATCCTAATAAAGTATATCATCACAATCTTTGGGTTGCCCGCCGCTCTCTCTTCTTCACTGCTCTGTCTCTTCCCGAGACTCTATAGAGGTTTACTTTCAACAATGTCGTCCATGTCAGAGTCTCGTGCTTTCCTGTTCTGCAACAGCTGTGGGTATATGCTATCTTTGACGACAACTCCTTATGCTAAACCTGGCCGATGCCCCTATTGCGATCATCCGCGACCTATAGAAGGGCTCTCCAAATGTAAATATTCGTACACAGTCAGTAAAGAGGATATTCAGAGGGAGCTAGGCAAATCAACAATTAAGGAGGAAAAGACTGAATTGGCAAAGACGGACATGAAAAAATGTGAAAAGTGTGGCCACAATGAGCATACATATTTTTCTAGACAGATGAGATCAGCTGATGAGGGGGCAACTACTTTCTACACTTGCACCAAGTGCAATAATAGTTTCAGTGAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020
Pfam Domains
Protein Families
TFIIS_C

Protein Analysis

210

Amino Acids

23.83

Weight (kDa)

6.96

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_TFIIS PF01096 169 - 206 7.9e-17 Transcription factor S-II (TFIIS)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 206
AccII CGCG 1 cut(s) 382
AciI CCGC 3 cut(s) 201, 204, 380
AcoI YGGCCR 3 cut(s) 17, 355, 523
AcuI CTGAAG 1 cut(s) 27
AdeI CACNNNGTG 1 cut(s) 603
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 1 cut(s) 392
AgsI TTSAA 1 cut(s) 261
AjnI CCWGG 2 cut(s) 14, 352
AluBI AGCT 3 cut(s) 309, 451, 566
AluI AGCT 3 cut(s) 309, 451, 566
Alw26I GTCTC 3 cut(s) 232, 233, 288
Ama87I CYCGRG 1 cut(s) 235
AoxI GGCC 3 cut(s) 17, 355, 523
AsuHPI GGTGA 1 cut(s) 146
AvaI CYCGRG 1 cut(s) 235
BalI TGGCCA 2 cut(s) 19, 525
BanII GRGCYC 1 cut(s) 399
BauI CACGAG 1 cut(s) 285
BciT130I CCWGG 2 cut(s) 16, 354
BcoDI GTCTC 3 cut(s) 232, 233, 288
BfaI CTAG 2 cut(s) 452, 549
BfmI CTRYAG 2 cut(s) 244, 387
BisI GCNGC 1 cut(s) 204
BlsI GCNGC 1 cut(s) 205
Bme1390I CCNGG 2 cut(s) 16, 354
BmeT110I CYCGRG 1 cut(s) 235
BmrFI CCNGG 2 cut(s) 16, 354
BmsI GCATC 1 cut(s) 350
BsaJI CCNNGG 1 cut(s) 116
BsaXI ACNNNNNCTCC 2 cut(s) 322, 352
Bsc4I CCNNNNNNNGG 1 cut(s) 392
BseBI CCWGG 2 cut(s) 16, 354
BseDI CCNNGG 1 cut(s) 116
BseGI GGATG 2 cut(s) 162, 376
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMII CTCAG 1 cut(s) 96
Bsh1236I CGCG 1 cut(s) 382
BshFI GGCC 3 cut(s) 19, 357, 525
BsiHKCI CYCGRG 1 cut(s) 235
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 3 cut(s) 232, 233, 288
BsnI GGCC 3 cut(s) 19, 357, 525
BsoBI CYCGRG 1 cut(s) 235
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 2 cut(s) 373, 560
Bsp19I CCATGG 1 cut(s) 116
BspACI CCGC 3 cut(s) 201, 204, 380
BspANI GGCC 3 cut(s) 19, 357, 525
BspCNI CTCAG 1 cut(s) 97
BspFNI CGCG 1 cut(s) 382
BspHI TCATGA 1 cut(s) 90
BsrBI CCGCTC 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 116
BssMI GATC 2 cut(s) 373, 560
BssSI CACGAG 1 cut(s) 285
BssT1I CCWWGG 1 cut(s) 116
Bst2BI CACGAG 1 cut(s) 285
Bst2UI CCWGG 2 cut(s) 16, 354
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 2 cut(s) 217, 236
BstAPI GCANNNNNTGC 2 cut(s) 129, 603
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 105
BstDSI CCRYGG 1 cut(s) 116
BstENI CCTNNNNNAGG 1 cut(s) 390
BstF5I GGATG 2 cut(s) 162, 376
BstFNI CGCG 1 cut(s) 382
BstKTI GATC 2 cut(s) 376, 563
BstMAI GTCTC 3 cut(s) 232, 233, 288
BstMBI GATC 2 cut(s) 373, 560
BstMWI GCNNNNNNNGC 3 cut(s) 129, 369, 603
BstNI CCWGG 2 cut(s) 16, 354
BstSCI CCNGG 2 cut(s) 14, 352
BstSFI CTRYAG 2 cut(s) 244, 387
BstUI CGCG 1 cut(s) 382
BsuRI GGCC 3 cut(s) 19, 357, 525
BtgI CCRYGG 1 cut(s) 116
BtsCI GGATG 2 cut(s) 162, 376
BtsI GCAGTG 1 cut(s) 218
BtsIMutI CAGTG 2 cut(s) 218, 625
Cac8I GCNNGC 1 cut(s) 201
CciI TCATGA 1 cut(s) 90
Csp6I GTAC 1 cut(s) 418
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviAII CATG 4 cut(s) 91, 117, 274, 502
CviJI RGCY 8 cut(s) 19, 69, 309, 357, 397, 451, 525, 566
CviKI_1 RGCY 8 cut(s) 19, 69, 309, 357, 397, 451, 525, 566
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 1 cut(s) 105
DpnI GATC 2 cut(s) 375, 562
DpnII GATC 2 cut(s) 373, 560
DraIII CACNNNGTG 1 cut(s) 603
EaeI YGGCCR 3 cut(s) 17, 355, 523
Eam1104I CTCTTC 2 cut(s) 217, 236
EarI CTCTTC 2 cut(s) 217, 236
Eco130I CCWWGG 1 cut(s) 116
Eco24I GRGCYC 1 cut(s) 399
Eco32I GATATC 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 390
EcoRII CCWGG 2 cut(s) 14, 352
EcoRV GATATC 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 116
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 116
FaeI CATG 4 cut(s) 94, 120, 277, 505
FatI CATG 4 cut(s) 90, 116, 273, 501
FauI CCCGC 1 cut(s) 208
Fnu4HI GCNGC 1 cut(s) 204
FokI GGATG 2 cut(s) 149, 363
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 1 cut(s) 204
FspBI CTAG 2 cut(s) 452, 549
GluI GCNGC 1 cut(s) 204
HaeIII GGCC 3 cut(s) 19, 357, 525
Hin1II CATG 4 cut(s) 94, 120, 277, 505
HinfI GANTC 2 cut(s) 240, 281
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 2 cut(s) 254, 420
Hpy188I TCNGA 5 cut(s) 7, 79, 106, 280, 444
Hpy188III TCNNGA 3 cut(s) 91, 235, 549
Hpy8I GTNNAC 2 cut(s) 254, 420
HpyAV CCTTC 1 cut(s) 386
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4V TGCA 4 cut(s) 42, 303, 597, 606
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 369, 603
HpyF3I CTNAG 1 cut(s) 105
Hsp92II CATG 4 cut(s) 94, 120, 277, 505
Kzo9I GATC 2 cut(s) 373, 560
LmnI GCTCC 3 cut(s) 64, 120, 448
LpnPI CCDG 5 cut(s) 28, 69, 308, 339, 366
LweI GCATC 1 cut(s) 350
MaeI CTAG 2 cut(s) 452, 549
MalI GATC 2 cut(s) 375, 562
MbiI CCGCTC 1 cut(s) 206
MboI GATC 2 cut(s) 373, 560
MboII GAAGA 4 cut(s) 20, 204, 207, 223
MhlI GDGCHC 1 cut(s) 399
MlsI TGGCCA 2 cut(s) 19, 525
MluCI AATT 5 cut(s) 28, 124, 465, 485, 625
MluNI TGGCCA 2 cut(s) 19, 525
MlyI GAGTC 2 cut(s) 234, 290
MnlI CCTC 9 cut(s) 29, 98, 110, 167, 242, 427, 438, 466, 565
Mox20I TGGCCA 2 cut(s) 19, 525
MscI TGGCCA 2 cut(s) 19, 525
MseI TTAA 2 cut(s) 468, 628
MslI CAYNNNNRTG 1 cut(s) 78
Msp20I TGGCCA 2 cut(s) 19, 525
MspA1I CMGCKG 2 cut(s) 309, 566
MspR9I CCNGG 2 cut(s) 16, 354
MvaI CCWGG 2 cut(s) 16, 354
MvnI CGCG 1 cut(s) 382
MwoI GCNNNNNNNGC 3 cut(s) 129, 369, 603
NcoI CCATGG 1 cut(s) 116
NdeII GATC 2 cut(s) 373, 560
NlaIII CATG 4 cut(s) 94, 120, 277, 505
PagI TCATGA 1 cut(s) 90
PkrI GCNGC 1 cut(s) 205
PleI GAGTC 2 cut(s) 234, 289
PpsI GAGTC 2 cut(s) 234, 289
Psp6I CCWGG 2 cut(s) 14, 352
PspGI CCWGG 2 cut(s) 14, 352
PvuII CAGCTG 2 cut(s) 309, 566
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 1 cut(s) 78
SaqAI TTAA 2 cut(s) 468, 628
SatI GCNGC 1 cut(s) 204
Sau3AI GATC 2 cut(s) 373, 560
SchI GAGTC 2 cut(s) 234, 290
ScrFI CCNGG 2 cut(s) 16, 354
SduI GDGCHC 1 cut(s) 399
SfaNI GCATC 1 cut(s) 350
SfcI CTRYAG 2 cut(s) 244, 387
SmiMI CAYNNNNRTG 1 cut(s) 78
Sse9I AATT 5 cut(s) 28, 124, 465, 485, 625
SsiI CCGC 3 cut(s) 201, 204, 380
SspI AATATT 1 cut(s) 413
SspMI CTAG 2 cut(s) 452, 549
StyD4I CCNGG 2 cut(s) 14, 352
StyI CCWWGG 1 cut(s) 116
TaaI ACNGT 1 cut(s) 424
TasI AATT 5 cut(s) 28, 124, 465, 485, 625
TauI GCSGC 1 cut(s) 206
Tru1I TTAA 2 cut(s) 468, 628
Tru9I TTAA 2 cut(s) 468, 628
TscAI CASTG 2 cut(s) 225, 625
TspDTI ATGAA 2 cut(s) 137, 518
TspGWI ACGGA 2 cut(s) 51, 512
TspRI CASTG 2 cut(s) 225, 625
XagI CCTNNNNNAGG 1 cut(s) 390
XbaI TCTAGA 1 cut(s) 548
XspI CTAG 2 cut(s) 452, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.