Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004196

Physical Location & Seq

Genomic Coordinates Reverse (-)

10193477 .. 10200046

Gene Structure 6570 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G27530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2745 bp

ATGGATTTGGCATCCCGTTATAAGGGGGTTGTTGGAATGGTTTTTGGCGACAATCAATCTTCTAATGAAGACAGTTACATTCAACGCCTGCTGGATCGTATAAGTAATGGTACCTTGCCTGACGATAGGAGAACTGCTATAGTAGAACTTCAGTCTGTTGTCGCAGAAAGTAATGCTGCTCAATTAGCTTTTGGAGCAGCCGGATTTCCTGTGATAGTGGGCATCCTAAAGGATCAACGAGATGATCTTGAAATGGTTCGAGGTGCTTTAGAAACTCTTCTTGGCGCTCTAACGCCAATCGATCATGCAAGGGCACAGAAGACTGAAGTCCAGGCAGCTCTTATGAACTCCGACTTGCTCTCCCGTGAAGCTGAAAATATCACTCTTCTTTTGAGTTTGCTGGAGGAAGAAGACTTTTATGTTCGATATTACACTCTTCAGATTTTGACAGCTCTGCTTATGAACTCTCAAAACAGGTTGCAGGAAGCTATTTTGACCACTCCTCGCGGTATTACTCGGCTCATGGATATGCTAATGGATAGAGAGGTAATCCGGAACGAGGCTTTGTTGCTTCTTACGCACTTGACTCGTGAAGCAGAGGAGATCCAAAAAATTGTTGTCTTTGAAGGTGCTTTTGAAAAGATTTTTAGCATCATTAAGGAGGAAGGGGGGTCAGATGGCGATGTGGTTGTGCAGGACTGTCTGGAGTTGTTGAACAATCTTCTTCGCAGCAGTTCATCTAACCAGATATTACTAAGGGAAACCATGGGTTTTGAACCTATCATTTCAATTCTAAAACTGCGAGGGATTACATATAAGTTCACCCAGCAAAAGACTGTTAATCTGCTTAGTGCACTGGAGACCATCAATATGCTGATCATGGGACGTGCAGACACGGAGCCTGGAAAAGATTCAAACAAGCTGGCAAATCGAACAGTTTTAGTTCAGAAAAAACTGCTAGACTACCTACTGATGTTGGGTGTTGAAAGCCAATGGGCGCCTGTTGCTGTTCGCTGCATGACATTTAAATGCATTGGAGATCTAATTGATGGACATCCTAAGAACCGTGATATCCTTGCTAGCAAAGTTCTTGGTGAAGATCGGCAAGTAGAACCTGCACTCAATTCTATCCTCCGGATCATTTTACAGACGTCCAGTATTCAAGAGTTTGTTGCAGCTGATTACGTTTTCAAGACATTCTGTGAGAAAAATACTGAGGGTCAAACAATGCTAGCTTCGACTTTAATACCTCAACCACATCCCACATCCCGAGATCATCTAGAAGATGATGTTCACATGTCATTTGGAAGTATGTTGTTACGAGGCCTTTGTTCTGGTGAAGCTGATGGTGATCTTGAGACATGTTGCAGAGCTGCAAGCATTCTTTCTCATGTTGTCAAGGACAATCTTCGGTGCAAGGAAAAGGCTTTGAAAATCGTACTTGAATCACCGATGCCGTCCATGGGAACTCCAGAGCCTCTCTTTCAGAGGATTGTCAGATACCTGGCTGTTGCTTCTTCCATGAAAAGCAAAGAGAAATCAAGCACACTGGGGAAATCATATATTCAACAGATCATTTTAAAACTGCTGGTGACATGGACTGTTGATTGTCCGACTGCAGTTCAGTGCTTTTTAGATTCCCGCCACCACCTAACGTTTTTGCTCGAGCTGGTAACAGACCCAGCTGCAACAGTTTGCATAAGGGGATTGGCGTCTATTCTATTGGGAGAATGCGTCATTTACAATAAATCAATTGAGAATGGTAAAGATGCTTTTTCTGTAGTTGATGCTGTGGGCCAGAAGATGGGGCTGACATCATACTTCTCAAAGTTTGAAGAGATGCAGAACAGCTTCATCTTCTCTCCCTCCAAGAAACCTCCACAGGGTTATAAACCGTTGACAAGAACACCCACGCCGAGTGAAGCCGAGATTAATGAGGTGGATGAGGTAGATGAAATGGTTAAAGGGAATGAGGATCACCCAATGCTCTTATCTTTGTTTGATGCTAGTTTCATAGGCTTAGTGAAGAGCTTGGAAGGTAACATCAGAGAAAGAATCGTGGATGTGTATAGTCGGCCAAAAAGTGAGGTGGCTGTAGTACCAGCAGATTTGGAGCAGAAGAGCGGAGAAAATGAGAAAGACTACATCAACCGCTTGAAAGCCTTTATAGAGAAACAGTGCTCGGAGATTCAGAACCTTCTTGCTCGAAACGCTGCTTTGGCAGAAGACGTAGCTAGCTCTGGCAGGAACGAGCAATCTCAAGGATCAGAGCAAAGAGCAAGTACAGTAATGGACAAAGTTCAGATGGAAAGCATCAGACGAGAACTCCAAGAAACATCTCAGCGTTTAGAGACAGTGAAGGCTGAAAAAGCCAAGATCGAGTCTGAGGCATCAAGTAACAAGAACATGGCTGCAAAACTTGAGTTCGACCTAAAAAGCTTATCGGATGCATACAACAGTCTAGAACAGGCGAATTACCATCTTGAGCAGGAAGTGAAATCCTTGAAAGGAGGAGAGAGTCCCATGCAGTTTCCTGATATAGAAGCCATCAAGGAAGAAGTGAGAAAAGAAGCTCAGAAAGAGAGCGAAGACGAGTTAAATGACTTGTTAGTATGTTTGGGACAAGAGGAAAGCAAAGTCGAGAAACTAAGCGCGAAGCTGATAGAATTAGGAGTTGATGTTGATAAGCTTTTAGAGGATATTGGAGACGAATCAGAAGCTCAAGCGGAATCTGAAGAAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005795 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0007275 GO:0008104 GO:0008150 GO:0008565 GO:0009791 GO:0009987 GO:0012505 GO:0012506 GO:0012507 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0022406 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032527 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045056 GO:0045184 GO:0046907 GO:0048193 GO:0048211 GO:0048278 GO:0048856 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0061024 GO:0061025 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0140056

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Uso1_p115_C Uso1_p115_head

Protein Analysis

914

Amino Acids

101.83

Weight (kDa)

4.86

Isoelectric Point (pI)

39.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Uso1_p115_head	PF04869	371 - 684	5.4e-21	Uso1 / p115 like vesicle tethering protein, head region
Uso1_p115_C	PF04871	789 - 914	9.9e-30	Uso1 / p115 like vesicle tethering protein, C terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004196)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27530
fragaria_vesca	FvH4_1g06690 FvH4_1g06690 FvH4_6g51710 FvH4_6g51710 FvH4_6g51710 FvH4_6g51710
malus_domestica	MD09G1023300.v1.1 MD17G1022800.v1.1
prunus_persica	Prupe.3G293500_v2.0.a1 Prupe.3G293600_v2.0.a1
pyrus_communis	pycom111g01940
rosa_chinensis	RchiOBHm_Chr2g0173241 RchiOBHm_Chr2g0173251
rosa_laevigata	RLG00000022173 RLG00000022175
rosa_multiflora	Rmu_sc0010961.1_g000008 Rmu_sc0010961.1_g000011
rosa_roxburghii	Rroxscaffold_2G00079090
rosa_rugosa	Rorug02G0566800 Rorug02G0566900 Rorug02G0566900 Rorug02G0566900
rosa_samantha	Rh2AG646700 Rh2BG657400 Rh2CG622600 Rh2CG623100 Rh2CG623200 Rh2DG671000 Rh2DG671500 Rh2DG671600
rosa_wichuraiana	Rw2G053120 Rw2G053130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	21, 1890
AatII	GACGTC	1 cut(s)	1154
Acc36I	ACCTGC	1 cut(s)	1123
Acc65I	GGTACC	1 cut(s)	110
AccB1I	GGYRCC	2 cut(s)	110, 997
AccB7I	CCANNNNNTGG	1 cut(s)	1804
AccBSI	CCGCTC	1 cut(s)	2124
AccII	CGCG	2 cut(s)	507, 2654
AccIII	TCCGGA	2 cut(s)	552, 1134
AciI	CCGC	5 cut(s)	507, 1642, 2124, 2152, 2726
AclI	AACGTT	1 cut(s)	1655
AclWI	GGATC	6 cut(s)	102, 240, 598, 1145, 1983, 2272
AcoI	YGGCCR	1 cut(s)	2075
AcuI	CTGAAG	3 cut(s)	134, 345, 422
AcyI	GRCGYC	3 cut(s)	998, 1151, 1712
AfaI	GTAC	4 cut(s)	112, 1440, 2100, 2284
AfiI	CCNNNNNNNGG	5 cut(s)	22, 559, 1463, 1804, 1883
AflIII	ACRYGT	2 cut(s)	1296, 1361
AjiI	CACGTC	1 cut(s)	887
AjnI	CCWGG	3 cut(s)	330, 901, 1503
AjuI	GAANNNNNNNTTGG	2 cut(s)	264, 296
Alw21I	GWGCWC	2 cut(s)	856, 2183
Alw26I	GTCTC	4 cut(s)	854, 1352, 2345, 2700
Alw44I	GTGCAC	1 cut(s)	852
AlwI	GGATC	6 cut(s)	102, 240, 598, 1145, 1983, 2272
Ama87I	CYCGRG	2 cut(s)	1269, 1664
Aor13HI	TCCGGA	2 cut(s)	552, 1134
AoxI	GGCC	3 cut(s)	1324, 1795, 2075
ApaLI	GTGCAC	1 cut(s)	852
AseI	ATTAAT	1 cut(s)	1932
Asp700I	GAANNNNTTC	4 cut(s)	255, 276, 910, 1850
Asp718I	GGTACC	1 cut(s)	110
AspLEI	GCGC	3 cut(s)	287, 1000, 2654
AspS9I	GGNCC	1 cut(s)	1795
AsuHPI	GGTGA	7 cut(s)	814, 1106, 1349, 1361, 1440, 1603, 1970
AsuNHI	GCTAGC	3 cut(s)	1079, 1231, 2234
AvaI	CYCGRG	2 cut(s)	1269, 1664
BaeGI	GKGCMC	2 cut(s)	316, 856
BanI	GGYRCC	2 cut(s)	110, 997
BauI	CACGAG	1 cut(s)	588
BbsI	GAAGAC	5 cut(s)	75, 326, 417, 2232, 2595
Bbv12I	GWGCWC	2 cut(s)	856, 2183
BccI	CCATC	8 cut(s)	671, 872, 1043, 1340, 1798, 2299, 2487, 2555
BceAI	ACGGC	1 cut(s)	1441
BcgI	CGANNNNNNTGC	2 cut(s)	569, 603
BciT130I	CCWGG	3 cut(s)	332, 903, 1505
BclI	TGATCA	1 cut(s)	876
BcoDI	GTCTC	4 cut(s)	854, 1352, 2345, 2700
BfaI	CTAG	7 cut(s)	959, 1080, 1232, 1280, 2007, 2235, 2462
BfmI	CTRYAG	4 cut(s)	138, 1617, 1779, 2094
BfoI	RGCGCY	2 cut(s)	288, 1001
BfuAI	ACCTGC	1 cut(s)	1123
BglII	AGATCT	1 cut(s)	1039
Bme1390I	CCNGG	3 cut(s)	332, 903, 1505
BmeT110I	CYCGRG	2 cut(s)	1269, 1664
BmgBI	CACGTC	1 cut(s)	887
BmgT120I	GGNCC	1 cut(s)	1795
BmiI	GGNNCC	3 cut(s)	112, 900, 999
BmrFI	CCNGG	3 cut(s)	332, 903, 1505
BmrI	ACTGGG	1 cut(s)	1559
BmtI	GCTAGC	3 cut(s)	1083, 1235, 2238
BmuI	ACTGGG	1 cut(s)	1559
BoxI	GACNNNNGTC	1 cut(s)	326
BpiI	GAAGAC	5 cut(s)	75, 326, 417, 2232, 2595
BpmI	CTGGAG	4 cut(s)	422, 725, 878, 1455
BpuEI	CTTGAG	5 cut(s)	1376, 2244, 2441, 2504, 2706
Bsa29I	ATCGAT	1 cut(s)	300
BsaBI	GATNNNNATC	2 cut(s)	1053, 1350
BsaHI	GRCGYC	3 cut(s)	998, 1151, 1712
BsaI	GGTCTC	1 cut(s)	854
BsaJI	CCNNGG	2 cut(s)	765, 1461
BsaWI	WCCGGW	2 cut(s)	552, 1134
BsaXI	ACNNNNNCTCC	8 cut(s)	344, 374, 1719, 1749, 2310, 2340, 2664, 2694
Bsc4I	CCNNNNNNNGG	5 cut(s)	22, 559, 1463, 1804, 1883
Bse1I	ACTGG	3 cut(s)	861, 1155, 1554
Bse8I	GATNNNNATC	2 cut(s)	1053, 1350
BseAI	TCCGGA	2 cut(s)	552, 1134
BseBI	CCWGG	3 cut(s)	332, 903, 1505
BseCI	ATCGAT	1 cut(s)	300
BseDI	CCNNGG	2 cut(s)	765, 1461
BseGI	GGATG	8 cut(s)	11, 222, 1054, 1258, 1265, 1948, 2068, 2452
BseJI	GATNNNNATC	2 cut(s)	1053, 1350
BseLI	CCNNNNNNNGG	5 cut(s)	22, 559, 1463, 1804, 1883
BseMII	CTCAG	4 cut(s)	1206, 2354, 2376, 2588
BseNI	ACTGG	3 cut(s)	861, 1155, 1554
BseRI	GAGGAG	3 cut(s)	492, 614, 2526
BseSI	GKGCMC	2 cut(s)	316, 856
BseYI	CCCAGC	2 cut(s)	825, 1681
BsgI	GTGCAG	3 cut(s)	713, 909, 1101
Bsh1236I	CGCG	2 cut(s)	507, 2654
BshFI	GGCC	3 cut(s)	1326, 1797, 2077
BshNI	GGYRCC	2 cut(s)	110, 997
BshVI	ATCGAT	1 cut(s)	300
BsiHKAI	GWGCWC	2 cut(s)	856, 2183
BsiHKCI	CYCGRG	2 cut(s)	1269, 1664
BsiSI	CCGG	3 cut(s)	201, 553, 1135
BslFI	GGGAC	3 cut(s)	897, 2505, 2634
BslI	CCNNNNNNNGG	5 cut(s)	22, 559, 1463, 1804, 1883
BsmAI	GTCTC	4 cut(s)	854, 1352, 2345, 2700
BsmBI	CGTCTC	1 cut(s)	2700
BsmFI	GGGAC	3 cut(s)	897, 2505, 2634
BsmI	GAATGC	2 cut(s)	1380, 1736
BsnI	GGCC	3 cut(s)	1326, 1797, 2077
Bso31I	GGTCTC	1 cut(s)	854
BsoBI	CYCGRG	2 cut(s)	1269, 1664
Bsp1286I	GDGCHC	3 cut(s)	316, 856, 2183
Bsp13I	TCCGGA	2 cut(s)	552, 1134
Bsp19I	CCATGG	2 cut(s)	765, 1461
BspACI	CCGC	5 cut(s)	507, 1642, 2124, 2152, 2726
BspANI	GGCC	3 cut(s)	1326, 1797, 2077
BspCNI	CTCAG	4 cut(s)	1207, 2353, 2377, 2587
BspDI	ATCGAT	1 cut(s)	300
BspEI	TCCGGA	2 cut(s)	552, 1134
BspFNI	CGCG	2 cut(s)	507, 2654
BspLI	GGNNCC	3 cut(s)	112, 900, 999
BspMAI	CTGCAG	1 cut(s)	1621
BspMI	ACCTGC	1 cut(s)	1123
BspOI	GCTAGC	3 cut(s)	1083, 1235, 2238
BspPI	GGATC	6 cut(s)	102, 240, 598, 1145, 1983, 2272
BspQI	GCTCTTC	2 cut(s)	2021, 2114
BspT107I	GGYRCC	2 cut(s)	110, 997
BspTNI	GGTCTC	1 cut(s)	854
BsrBI	CCGCTC	1 cut(s)	2124
BsrI	ACTGG	3 cut(s)	861, 1155, 1554
BssECI	CCNNGG	2 cut(s)	765, 1461
BssNI	GRCGYC	3 cut(s)	998, 1151, 1712
BssSI	CACGAG	1 cut(s)	588
BssT1I	CCWWGG	2 cut(s)	765, 1461
Bst2BI	CACGAG	1 cut(s)	588
Bst2UI	CCWGG	3 cut(s)	332, 903, 1505
Bst6I	CTCTTC	6 cut(s)	282, 390, 441, 1830, 2021, 2114
BstACI	GRCGYC	3 cut(s)	998, 1151, 1712
BstC8I	GCNNGC	6 cut(s)	89, 924, 1081, 1233, 1378, 2236
BstDEI	CTNAG	9 cut(s)	755, 848, 1059, 1215, 2020, 2340, 2385, 2574, 2648
BstDSI	CCRYGG	2 cut(s)	765, 1461
BstF5I	GGATG	8 cut(s)	11, 222, 1054, 1258, 1265, 1948, 2068, 2452
BstFNI	CGCG	2 cut(s)	507, 2654
BstH2I	RGCGCY	2 cut(s)	288, 1001
BstHHI	GCGC	3 cut(s)	287, 1000, 2654
BstMAI	GTCTC	4 cut(s)	854, 1352, 2345, 2700
BstMWI	GCNNNNNNNGC	7 cut(s)	185, 194, 577, 1004, 2210, 2219, 2369
BstNI	CCWGG	3 cut(s)	332, 903, 1505
BstNSI	RCATGY	2 cut(s)	1300, 1365
BstPAI	GACNNNNGTC	1 cut(s)	326
BstSCI	CCNGG	3 cut(s)	330, 901, 1503
BstSFI	CTRYAG	4 cut(s)	138, 1617, 1779, 2094
BstSLI	GKGCMC	2 cut(s)	316, 856
BstUI	CGCG	2 cut(s)	507, 2654
BstV2I	GAAGAC	5 cut(s)	75, 326, 417, 2232, 2595
BstX2I	RGATCY	2 cut(s)	603, 1039
BstYI	RGATCY	2 cut(s)	603, 1039
Bsu15I	ATCGAT	1 cut(s)	300
BsuRI	GGCC	3 cut(s)	1326, 1797, 2077
BsuTUI	ATCGAT	1 cut(s)	300
BtgI	CCRYGG	2 cut(s)	765, 1461
BtgZI	GCGATG	1 cut(s)	696
BtrI	CACGTC	1 cut(s)	887
BtsCI	GGATG	8 cut(s)	11, 222, 1054, 1258, 1265, 1948, 2068, 2452
BtsIMutI	CAGTG	5 cut(s)	854, 1547, 1631, 2183, 2361
BveI	ACCTGC	1 cut(s)	1123
Cac8I	GCNNGC	6 cut(s)	89, 924, 1081, 1233, 1378, 2236
CfoI	GCGC	3 cut(s)	287, 1000, 2654
Cfr13I	GGNCC	1 cut(s)	1795
ClaI	ATCGAT	1 cut(s)	300
CseI	GACGC	2 cut(s)	1701, 1723
Csp6I	GTAC	4 cut(s)	111, 1439, 2099, 2283
CviQI	GTAC	4 cut(s)	111, 1439, 2099, 2283
DdeI	CTNAG	9 cut(s)	755, 848, 1059, 1215, 2020, 2340, 2385, 2574, 2648
DinI	GGCGCC	1 cut(s)	999
DraI	TTTAAA	2 cut(s)	1027, 1581
EaeI	YGGCCR	1 cut(s)	2075
Eam1104I	CTCTTC	6 cut(s)	282, 390, 441, 1830, 2021, 2114
EarI	CTCTTC	6 cut(s)	282, 390, 441, 1830, 2021, 2114
Eco130I	CCWWGG	2 cut(s)	765, 1461
Eco147I	AGGCCT	1 cut(s)	1326
Eco31I	GGTCTC	1 cut(s)	854
Eco32I	GATATC	1 cut(s)	1072
Eco57I	CTGAAG	3 cut(s)	134, 345, 422
Eco88I	CYCGRG	2 cut(s)	1269, 1664
EcoRII	CCWGG	3 cut(s)	330, 901, 1503
EcoRV	GATATC	1 cut(s)	1072
EcoT14I	CCWWGG	2 cut(s)	765, 1461
EcoT22I	ATGCAT	2 cut(s)	1034, 2452
EgeI	GGCGCC	1 cut(s)	999
EheI	GGCGCC	1 cut(s)	999
ErhI	CCWWGG	2 cut(s)	765, 1461
Esp3I	CGTCTC	1 cut(s)	2700
FaqI	GGGAC	3 cut(s)	897, 2505, 2634
FauI	CCCGC	1 cut(s)	1649
FbaI	TGATCA	1 cut(s)	876
FokI	GGATG	7 cut(s)	209, 1041, 1245, 1252, 1955, 2075, 2459
FspBI	CTAG	7 cut(s)	959, 1080, 1232, 1280, 2007, 2235, 2462
GlaI	GCGC	3 cut(s)	286, 999, 2653
GsaI	CCCAGC	2 cut(s)	829, 1685
GsuI	CTGGAG	4 cut(s)	422, 725, 878, 1455
HaeII	RGCGCY	2 cut(s)	288, 1001
HaeIII	GGCC	3 cut(s)	1326, 1797, 2077
HapII	CCGG	3 cut(s)	201, 553, 1135
HgaI	GACGC	2 cut(s)	1701, 1723
HhaI	GCGC	3 cut(s)	287, 1000, 2654
Hin1I	GRCGYC	3 cut(s)	998, 1151, 1712
Hin6I	GCGC	3 cut(s)	285, 998, 2652
HinP1I	GCGC	3 cut(s)	285, 998, 2652
HincII	GTYRAC	1 cut(s)	1899
HindII	GTYRAC	1 cut(s)	1899
HindIII	AAGCTT	2 cut(s)	2437, 2687
HpaII	CCGG	3 cut(s)	201, 553, 1135
HphI	GGTGA	7 cut(s)	814, 1106, 1349, 1361, 1440, 1603, 1970
Hpy166II	GTNNAC	4 cut(s)	822, 854, 1294, 1899
Hpy8I	GTNNAC	4 cut(s)	822, 854, 1294, 1899
HpyAV	CCTTC	5 cut(s)	620, 659, 2030, 2207, 2353
HpyCH4IV	ACGT	5 cut(s)	886, 1151, 1185, 1655, 2229
HpyF10VI	GCNNNNNNNGC	7 cut(s)	185, 194, 577, 1004, 2210, 2219, 2369
HpyF3I	CTNAG	9 cut(s)	755, 848, 1059, 1215, 2020, 2340, 2385, 2574, 2648
HpySE526I	ACGT	5 cut(s)	886, 1151, 1185, 1655, 2229
Hsp92I	GRCGYC	3 cut(s)	998, 1151, 1712
HspAI	GCGC	3 cut(s)	285, 998, 2652
KasI	GGCGCC	1 cut(s)	997
Kpn2I	TCCGGA	2 cut(s)	552, 1134
KpnI	GGTACC	1 cut(s)	114
Ksp22I	TGATCA	1 cut(s)	876
LguI	GCTCTTC	2 cut(s)	2021, 2114
LmnI	GCTCC	3 cut(s)	194, 898, 2113
MaeI	CTAG	7 cut(s)	959, 1080, 1232, 1280, 2007, 2235, 2462
MaeII	ACGT	5 cut(s)	886, 1151, 1185, 1655, 2229
MaeIII	GTNAC	6 cut(s)	74, 1317, 1591, 1672, 2039, 2396
MbiI	CCGCTC	1 cut(s)	2124
MfeI	CAATTG	1 cut(s)	1752
MflI	RGATCY	2 cut(s)	603, 1039
MhlI	GDGCHC	3 cut(s)	316, 856, 2183
MluCI	AATT	8 cut(s)	182, 612, 789, 1044, 1123, 1752, 2473, 2666
Mly113I	GGCGCC	1 cut(s)	998
MlyI	GAGTC	3 cut(s)	580, 2390, 2527
MmeI	TCCRAC	3 cut(s)	13, 375, 1637
Mph1103I	ATGCAT	2 cut(s)	1034, 2452
MroI	TCCGGA	2 cut(s)	552, 1134
MroXI	GAANNNNTTC	4 cut(s)	255, 276, 910, 1850
MseI	TTAA	8 cut(s)	657, 840, 1026, 1244, 1580, 1932, 1962, 2597
MslI	CAYNNNNRTG	3 cut(s)	527, 869, 1027
MspA1I	CMGCKG	2 cut(s)	1178, 1685
MspI	CCGG	3 cut(s)	201, 553, 1135
MspR9I	CCNGG	3 cut(s)	332, 903, 1505
MunI	CAATTG	1 cut(s)	1752
Mva1269I	GAATGC	2 cut(s)	1380, 1736
MvaI	CCWGG	3 cut(s)	332, 903, 1505
MvnI	CGCG	2 cut(s)	507, 2654
MwoI	GCNNNNNNNGC	7 cut(s)	185, 194, 577, 1004, 2210, 2219, 2369
NarI	GGCGCC	1 cut(s)	998
NcoI	CCATGG	2 cut(s)	765, 1461
NheI	GCTAGC	3 cut(s)	1079, 1231, 2234
NlaIV	GGNNCC	3 cut(s)	112, 900, 999
NmeAIII	GCCGAG	3 cut(s)	496, 1941, 1951
NmuCI	GTSAC	1 cut(s)	1591
NsiI	ATGCAT	2 cut(s)	1034, 2452
NspI	RCATGY	2 cut(s)	1300, 1365
PaeR7I	CTCGAG	1 cut(s)	1664
PceI	AGGCCT	1 cut(s)	1326
PciI	ACATGT	2 cut(s)	1296, 1361
PciSI	GCTCTTC	2 cut(s)	2021, 2114
PctI	GAATGC	2 cut(s)	1380, 1736
PdmI	GAANNNNTTC	4 cut(s)	255, 276, 910, 1850
PfeI	GAWTC	7 cut(s)	911, 1445, 1637, 2055, 2188, 2711, 2729
PflMI	CCANNNNNTGG	1 cut(s)	1804
PleI	GAGTC	3 cut(s)	580, 2389, 2526
PluTI	GGCGCC	1 cut(s)	1001
PpsI	GAGTC	3 cut(s)	580, 2389, 2526
PscI	ACATGT	2 cut(s)	1296, 1361
PshAI	GACNNNNGTC	1 cut(s)	326
PshBI	ATTAAT	1 cut(s)	1932
PsiI	TTATAA	2 cut(s)	21, 1890
Psp1406I	AACGTT	1 cut(s)	1655
Psp6I	CCWGG	3 cut(s)	330, 901, 1503
PspFI	CCCAGC	2 cut(s)	825, 1681
PspGI	CCWGG	3 cut(s)	330, 901, 1503
PspN4I	GGNNCC	3 cut(s)	112, 900, 999
PspPI	GGNCC	1 cut(s)	1795
PspXI	VCTCGAGB	1 cut(s)	1664
PstI	CTGCAG	1 cut(s)	1621
PsuI	RGATCY	2 cut(s)	603, 1039
PvuII	CAGCTG	2 cut(s)	1178, 1685
RsaI	GTAC	4 cut(s)	112, 1440, 2100, 2284
RsaNI	GTAC	4 cut(s)	111, 1439, 2099, 2283
RseI	CAYNNNNRTG	3 cut(s)	527, 869, 1027
SapI	GCTCTTC	2 cut(s)	2021, 2114
SaqAI	TTAA	8 cut(s)	657, 840, 1026, 1244, 1580, 1932, 1962, 2597
Sau96I	GGNCC	1 cut(s)	1795
SchI	GAGTC	3 cut(s)	580, 2390, 2527
ScrFI	CCNGG	3 cut(s)	332, 903, 1505
SduI	GDGCHC	3 cut(s)	316, 856, 2183
SfcI	CTRYAG	4 cut(s)	138, 1617, 1779, 2094
SfoI	GGCGCC	1 cut(s)	999
Sfr274I	CTCGAG	1 cut(s)	1664
SlaI	CTCGAG	1 cut(s)	1664
SmiI	ATTTAAAT	1 cut(s)	1027
SmiMI	CAYNNNNRTG	3 cut(s)	527, 869, 1027
SmlI	CTYRAG	6 cut(s)	1355, 1664, 2259, 2420, 2483, 2721
SmoI	CTYRAG	6 cut(s)	1355, 1664, 2259, 2420, 2483, 2721
Sse9I	AATT	8 cut(s)	182, 612, 789, 1044, 1123, 1752, 2473, 2666
SseBI	AGGCCT	1 cut(s)	1326
SsiI	CCGC	5 cut(s)	507, 1642, 2124, 2152, 2726
SspDI	GGCGCC	1 cut(s)	997
SspMI	CTAG	7 cut(s)	959, 1080, 1232, 1280, 2007, 2235, 2462
StuI	AGGCCT	1 cut(s)	1326
StyD4I	CCNGG	3 cut(s)	330, 901, 1503
StyI	CCWWGG	2 cut(s)	765, 1461
SwaI	ATTTAAAT	1 cut(s)	1027
TaiI	ACGT	5 cut(s)	889, 1154, 1188, 1658, 2232
TasI	AATT	8 cut(s)	182, 612, 789, 1044, 1123, 1752, 2473, 2666
TatI	WGTACW	1 cut(s)	2282
TfiI	GAWTC	7 cut(s)	911, 1445, 1637, 2055, 2188, 2711, 2729
Tru1I	TTAA	8 cut(s)	657, 840, 1026, 1244, 1580, 1932, 1962, 2597
Tru9I	TTAA	8 cut(s)	657, 840, 1026, 1244, 1580, 1932, 1962, 2597
TscAI	CASTG	5 cut(s)	861, 1554, 1631, 2183, 2361
TseFI	GTSAC	1 cut(s)	1591
Tsp45I	GTSAC	1 cut(s)	1591
TspDTI	ATGAA	8 cut(s)	81, 359, 476, 726, 1538, 1843, 1968, 2002
TspGWI	ACGGA	1 cut(s)	911
TspRI	CASTG	5 cut(s)	861, 1554, 1631, 2183, 2361
Van91I	CCANNNNNTGG	1 cut(s)	1804
VneI	GTGCAC	1 cut(s)	852
VspI	ATTAAT	1 cut(s)	1932
XbaI	TCTAGA	2 cut(s)	1279, 2461
XceI	RCATGY	2 cut(s)	1300, 1365
XhoI	CTCGAG	1 cut(s)	1664
XmnI	GAANNNNTTC	4 cut(s)	255, 276, 910, 1850
XspI	CTAG	7 cut(s)	959, 1080, 1232, 1280, 2007, 2235, 2462
ZraI	GACGTC	1 cut(s)	1152
Zsp2I	ATGCAT	2 cut(s)	1034, 2452

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.