Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0004196

Physical Location & Seq

Genomic Coordinates Forward (+)

89394240 .. 89399905

Gene Structure 5666 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG671000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGAAACCGGGCATCGGCGTCACCAGCTTTATTGATCGTCCTAACTTCTCAGGGATGGATTTGGTGAATAGTTACAAGGGGGTTGTAGGTCTCGTCTTCGGCAATGAGAAATCTTCTTCTTCAAATGAAGACAGTTATGTTGAGCGATTGCTGGACTGTATAAGCAATGGGAAGCTGGCAGAGGACAGGAGGACTGCTATGGTGGAGCTTCAGTCCGTTGTGGCAGAGAGCTCTGGTGCTCAGCTGGCATTTGGAGCAATGGGCTTTCCTGTGATGATGGGTGTATTGAGAGAAGAGCGCGACGATGTTGAAATGGTTCGAGGTGCCCTTGAAACTCTTGTAGGTGCATTGACTCCTATTGAACATGCAAAAATCCCAAAGAATGAGATTCAACCTGCTCTAATGAACACTGATTTGCTTTCCAGAGAAGCAGACAGCATTTCACTTCTCTTAAGTTTGTTGTCAGAGGAGGATTTCTATGTACGATATTACACTCTTCAACTTTTGACAGCTCTTCTCACAAATTCTCCAAATAGGTTACAGGAAGCCATTCTGACCATACCTCGTGGTATAACTCGCCTAATGGATATGCTTATGGATCGGGAGGTTATACGAAATGAGGCCTTATTACTTCTTACTTACTTGACACGTGAAGCTGAGGAAATTCAAAAAATTGTGGTCTTTGAAGGTGCTTTTGAAAAGATTTTCAGCATCATCAAAGAGGAAGGTGGCTCAGACGGTGGTGTTGTTGTCCAGGACTGTATTGAACTGTTAAACAACCTTATTCGGAAGAATGCATCTAATCAGATACTATTGAGGGAGACAATTGGATTTGACCCATTAATGTCAATTTTGAAGTTACGAGGAAGTGCTTACAGTTTCACTCAACAAAAGACAATTAATCTACTCAGTTCGTTGGAAACTATTAATTTGCTGATAATGGGAGGTTCAGAGGCTGATCCTGGAAAAGATGCAAATAAGCTGACAAACAAGACAACTCTTGTTCAGAAAAAAATCTTGGACCATCTTCTAATGCTGGGTGTAGAAAGCCAGTGGGCACCTGTAGCTGTCCGTTGTGCAGCATTACAATGCATCGGTAATCTAATTATCGGACATTCCAAGAATCTTGATGCCCTTGCAAGCAAAGTTCTCGGAGAGGGGCCACAGGAAGCTGCTCTGAACTCAATTCTACGAATCATCTTAAGGACATCTAGTGTACAAGAATTTGTTGCAGCTGATTATGTTTTTAAGAGCTTTTGTGAGAAAAATGCTGATGGCCAAACAATGTTAGCATCAACATTGATTCCTCAACCACATTCAATGACTCATGCACCCCTTGAGGAAGATGTGAACATGTCATTTGGAAGCATGCTGTTACAAGGTCTTACCTTGAATGAGAGTGAAGTCGATCTTGAGACATGTTGCAGAGCTGCCAGTGTTCTATCTCATGTCATGAAGGACAATATCCAGTGCAAGGAAAAGGTGTTGCATATTGACCTTGAGGCACCTACACCATCTTTAGGCGCTTCTGAGCCTCTAATGCATCGCGTGGTAAAATACTTGGCCCTTTCCTCTTCCATGAAAAGCAAAGATGGAAAGTCAGGTGGAAATGCATATGTTCAACCAATCCTCTTGAAAATGCTGGTGACCTGGCTAGCTGATTGCCCTAGTGCTGTTCATTGCTTCCTAGATTCACGTCCCCACATCACATATCTGCTTGAACTGGTATCCAGTTCATCCGCTACAGTGTTCGTAAAGGGATTGGCGGCTGTTCTTCTGGGAGAGTGTGTTATCTACAATAAATCAGGTGAAAGTGGGAAAGATGCGTTTACTGTAGTTGATTCCATAAGCCAGAAGGTTGGACTCACATCATACTTTCTGAAGTTTGATGAAATGAGAAAAAACTTCCTTTTCACCTCTGCAAGGTCTGCTGAGCCCCGTCAACAATTAACCAGATCTGCTTCTGCTGGTATGGTAGAGCCTGAAGATCTTGATGGGAACAATTTATCTGATCAGAAAGATGAGGATCTACCGGTTCTCTCCTCAATCTTTGATGCTGCATTTGTAAACCTTGTTAAGAGTTTAGAGGCAAACATCAGAGAAAAAATCGTTGAGGTTTACAGTAATCCAAAGAGCAAGGTGGCAGTGGTGCCAGCAGAATTGGAACAAAAGAGTGGGGAAAGTGATGGGGAGTATGTTAAGCGGCTGAAAGAATTTGTGGAAAAGCAGTGCTCTGAAATACAGGACCTCCTTGGCCGCAATGCAAGCTTGGCTGAGGACGTGGCAGCAACTGGTGGGGGAAGTCATTCTCGGTCTGAGCAAGGCACAGGCTCAGATAGAGTTCATGTAGAGACACTTAGACGAGATCTTCAAGAAGCATCCAGGCGGTTGGAGTTGCTCAAGGCAGAGAAAGCCAAGATTGAATCTGAGGCATCCATGTATAAAAATTTAGCTGGCAAGATGGAATCCGACCTGAAGAGTCTATCGGATGCTTACAACAGTCTTGAGCAGGCCAATTTCCAACTAGAAAAGGAGGTAAGAGGTCAAAAGGGTGTGGGGTCTTCAGCCTTTCCAGATGTGGATGCAATTAGAGCCCAGGCAAGGGAAGAAGCTCAGAAGGAGAGCGAGGCAGAACTGAATGATCTTCTCGTGTGCCTTGGACAGGAACAGAGCAAGGTCGAAAAGCTTAGTGCCAGGTTGTTGGAGTTGGGGGAGGACGTGGACAAGCTGCTTGAAGATATCGGAGATGATATGGGATTGCCTGGAGACGGTGAAGAGGAGGAAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005795 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0007275 GO:0008104 GO:0008150 GO:0008565 GO:0009791 GO:0009987 GO:0012505 GO:0012506 GO:0012507 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0022406 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032527 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045056 GO:0045184 GO:0046907 GO:0048193 GO:0048211 GO:0048278 GO:0048856 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0061024 GO:0061025 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0140056

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Uso1_p115_C Uso1_p115_head

Protein Analysis

929

Amino Acids

101.76

Weight (kDa)

4.86

Isoelectric Point (pI)

41.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Uso1_p115_head	PF04869	393 - 702	4.8e-25	Uso1 / p115 like vesicle tethering protein, head region
Uso1_p115_C	PF04871	803 - 927	9e-29	Uso1 / p115 like vesicle tethering protein, C terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004196)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27530
fragaria_vesca	FvH4_1g06690 FvH4_1g06690 FvH4_6g51710 FvH4_6g51710 FvH4_6g51710 FvH4_6g51710
malus_domestica	MD09G1023300.v1.1 MD17G1022800.v1.1
prunus_persica	Prupe.3G293500_v2.0.a1 Prupe.3G293600_v2.0.a1
pyrus_communis	pycom111g01940
rosa_chinensis	RchiOBHm_Chr2g0173241 RchiOBHm_Chr2g0173251
rosa_laevigata	RLG00000022173 RLG00000022175
rosa_multiflora	Rmu_sc0010961.1_g000008 Rmu_sc0010961.1_g000011
rosa_roxburghii	Rroxscaffold_2G00079090
rosa_rugosa	Rorug02G0566800 Rorug02G0566900 Rorug02G0566900 Rorug02G0566900
rosa_samantha	Rh2AG646700 Rh2BG657400 Rh2CG622600 Rh2CG623100 Rh2CG623200 Rh2DG671000 Rh2DG671500 Rh2DG671600
rosa_wichuraiana	Rw2G053120 Rw2G053130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	403
AccB1I	GGYRCC	4 cut(s)	323, 1057, 1504, 2149
AccII	CGCG	2 cut(s)	300, 1548
AciI	CCGC	5 cut(s)	1740, 1766, 2203, 2257, 2386
AclWI	GGATC	3 cut(s)	606, 953, 2034
AcoI	YGGCCR	2 cut(s)	1276, 2254
AcsI	RAATTY	5 cut(s)	523, 663, 1223, 2213, 2446
AcuI	CTGAAG	5 cut(s)	194, 1901, 2004, 2495, 2547
AcvI	CACGTG	1 cut(s)	650
AcyI	GRCGYC	1 cut(s)	18
AfaI	GTAC	2 cut(s)	483, 1218
AfiI	CCNNNNNNNGG	8 cut(s)	14, 1474, 1520, 1923, 2601, 2602, 2662, 2768
AflII	CTTAAG	2 cut(s)	451, 1201
AflIII	ACRYGT	3 cut(s)	647, 1353, 1418
AgeI	ACCGGT	1 cut(s)	2032
AjiI	CACGTC	3 cut(s)	1697, 2281, 2719
AjnI	CCWGG	7 cut(s)	753, 961, 1649, 2381, 2595, 2693, 2761
AjuI	GAANNNNNNNTTGG	2 cut(s)	1001, 1033
Alw21I	GWGCWC	3 cut(s)	233, 241, 2234
Alw26I	GTCTC	5 cut(s)	95, 815, 1409, 2345, 2760
AlwI	GGATC	3 cut(s)	606, 953, 2034
AlwNI	CAGNNNCTG	2 cut(s)	956, 2291
AoxI	GGCC	6 cut(s)	621, 1160, 1276, 1563, 2254, 2511
ApeKI	GCWGC	7 cut(s)	1079, 1172, 1232, 1430, 2057, 2285, 2728
ApoI	RAATTY	5 cut(s)	523, 663, 1223, 2213, 2446
AseI	ATTAAT	3 cut(s)	842, 900, 927
AsiGI	ACCGGT	1 cut(s)	2032
Asp700I	GAANNNNTTC	3 cut(s)	315, 549, 2305
AspLEI	GCGC	2 cut(s)	300, 1526
AspS9I	GGNCC	4 cut(s)	1021, 1160, 1564, 2245
AsuC2I	CCSGG	1 cut(s)	9
AsuHPI	GGTGA	6 cut(s)	13, 76, 1657, 1820, 1906, 2783
AsuNHI	GCTAGC	1 cut(s)	1654
AvaII	GGWCC	2 cut(s)	1021, 2245
BaeGI	GKGCMC	2 cut(s)	328, 1060
BalI	TGGCCA	1 cut(s)	1278
BanI	GGYRCC	4 cut(s)	323, 1057, 1504, 2149
BanII	GRGCYC	3 cut(s)	233, 1938, 2596
BauI	CACGAG	2 cut(s)	564, 2648
BbrPI	CACGTG	1 cut(s)	650
BbsI	GAAGAC	3 cut(s)	88, 135, 2553
Bbv12I	GWGCWC	3 cut(s)	233, 241, 2234
BbvCI	CCTCAGC	2 cut(s)	657, 2274
BbvI	GCAGC	7 cut(s)	1091, 1159, 1244, 1417, 2044, 2297, 2715
BccI	CCATC	9 cut(s)	49, 271, 1032, 1268, 1522, 1586, 1988, 2180, 2455
BcgI	CGANNNNNNTGC	2 cut(s)	1132, 1166
BciT130I	CCWGG	7 cut(s)	755, 963, 1651, 2383, 2597, 2695, 2763
BciVI	GTATCC	1 cut(s)	1738
BclI	TGATCA	1 cut(s)	2011
BcnI	CCSGG	1 cut(s)	9
BcoDI	GTCTC	5 cut(s)	95, 815, 1409, 2345, 2760
BfaI	CTAG	5 cut(s)	1212, 1655, 1668, 1688, 2525
BfmI	CTRYAG	3 cut(s)	1062, 1743, 1833
BfoI	RGCGCY	1 cut(s)	1527
BfrI	CTTAAG	2 cut(s)	451, 1201
BfuAI	ACCTGC	1 cut(s)	403
BfuI	GTATCC	1 cut(s)	1738
BglII	AGATCT	3 cut(s)	1955, 1987, 2365
BlpI	GCTNAGC	2 cut(s)	240, 1932
Bme1390I	CCNGG	8 cut(s)	9, 755, 963, 1651, 2383, 2597, 2695, 2763
Bme18I	GGWCC	2 cut(s)	1021, 2245
BmgBI	CACGTC	3 cut(s)	1697, 2281, 2719
BmgT120I	GGNCC	4 cut(s)	1021, 1160, 1564, 2245
BmiI	GGNNCC	5 cut(s)	325, 1059, 1161, 1506, 2151
BmrFI	CCNGG	8 cut(s)	9, 755, 963, 1651, 2383, 2597, 2695, 2763
BmtI	GCTAGC	1 cut(s)	1658
BpiI	GAAGAC	3 cut(s)	88, 135, 2553
BpmI	CTGGAG	1 cut(s)	2784
Bpu10I	CCTNAGC	2 cut(s)	657, 2274
Bpu1102I	GCTNAGC	2 cut(s)	240, 1932
BpuEI	CTTGAG	5 cut(s)	1358, 1433, 1520, 2384, 2525
BpuMI	CCSGG	1 cut(s)	9
BsaAI	YACGTR	1 cut(s)	650
BsaBI	GATNNNNATC	1 cut(s)	2025
BsaHI	GRCGYC	1 cut(s)	18
BsaI	GGTCTC	1 cut(s)	95
BsaJI	CCNNGG	3 cut(s)	2251, 2595, 2656
BsaWI	WCCGGW	1 cut(s)	2032
BsaXI	ACNNNNNCTCC	8 cut(s)	197, 227, 511, 541, 1773, 1803, 2232, 2262
Bsc4I	CCNNNNNNNGG	8 cut(s)	14, 1474, 1520, 1923, 2601, 2602, 2662, 2768
Bse118I	RCCGGY	1 cut(s)	2032
Bse1I	ACTGG	6 cut(s)	1051, 1434, 1468, 1728, 1731, 2296
Bse3DI	GCAATG	5 cut(s)	109, 172, 264, 1678, 2266
Bse8I	GATNNNNATC	1 cut(s)	2025
BseBI	CCWGG	7 cut(s)	755, 963, 1651, 2383, 2597, 2695, 2763
BseDI	CCNNGG	3 cut(s)	2251, 2595, 2656
BseGI	GGATG	6 cut(s)	60, 1736, 2378, 2432, 2494, 2587
BseJI	GATNNNNATC	1 cut(s)	2025
BseLI	CCNNNNNNNGG	8 cut(s)	14, 1474, 1520, 1923, 2601, 2602, 2662, 2768
BseMI	GCAATG	5 cut(s)	109, 172, 264, 1678, 2266
BseNI	ACTGG	6 cut(s)	1051, 1434, 1468, 1728, 1731, 2296
BseRI	GAGGAG	2 cut(s)	482, 2032
BseSI	GKGCMC	2 cut(s)	328, 1060
BseXI	GCAGC	7 cut(s)	1091, 1159, 1244, 1417, 2044, 2297, 2715
BseYI	CCCAGC	1 cut(s)	1036
BsgI	GTGCAG	1 cut(s)	1098
Bsh1236I	CGCG	2 cut(s)	300, 1548
BshFI	GGCC	6 cut(s)	623, 1162, 1278, 1565, 2256, 2513
BshNI	GGYRCC	4 cut(s)	323, 1057, 1504, 2149
BshTI	ACCGGT	1 cut(s)	2032
BsiHKAI	GWGCWC	3 cut(s)	233, 241, 2234
BsiSI	CCGG	2 cut(s)	8, 2033
BslFI	GGGAC	1 cut(s)	1683
BslI	CCNNNNNNNGG	8 cut(s)	14, 1474, 1520, 1923, 2601, 2602, 2662, 2768
BsmAI	GTCTC	5 cut(s)	95, 815, 1409, 2345, 2760
BsmBI	CGTCTC	1 cut(s)	2760
BsmFI	GGGAC	1 cut(s)	1683
BsmI	GAATGC	1 cut(s)	799
BsnI	GGCC	6 cut(s)	623, 1162, 1278, 1565, 2256, 2513
Bso31I	GGTCTC	1 cut(s)	95
Bsp1286I	GDGCHC	7 cut(s)	233, 241, 328, 1060, 1938, 2234, 2596
Bsp1407I	TGTACA	1 cut(s)	1216
Bsp1720I	GCTNAGC	2 cut(s)	240, 1932
BspACI	CCGC	5 cut(s)	1740, 1766, 2203, 2257, 2386
BspANI	GGCC	6 cut(s)	623, 1162, 1278, 1565, 2256, 2513
BspFNI	CGCG	2 cut(s)	300, 1548
BspHI	TCATGA	1 cut(s)	1452
BspLI	GGNNCC	5 cut(s)	325, 1059, 1161, 1506, 2151
BspMI	ACCTGC	1 cut(s)	403
BspOI	GCTAGC	1 cut(s)	1658
BspPI	GGATC	3 cut(s)	606, 953, 2034
BspQI	GCTCTTC	2 cut(s)	288, 519
BspT107I	GGYRCC	4 cut(s)	323, 1057, 1504, 2149
BspTI	CTTAAG	2 cut(s)	451, 1201
BspTNI	GGTCTC	1 cut(s)	95
BsrDI	GCAATG	5 cut(s)	109, 172, 264, 1678, 2266
BsrFI	RCCGGY	1 cut(s)	2032
BsrGI	TGTACA	1 cut(s)	1216
BsrI	ACTGG	6 cut(s)	1051, 1434, 1468, 1728, 1731, 2296
BssAI	RCCGGY	1 cut(s)	2032
BssECI	CCNNGG	3 cut(s)	2251, 2595, 2656
BssNI	GRCGYC	1 cut(s)	18
BssSI	CACGAG	2 cut(s)	564, 2648
BssT1I	CCWWGG	2 cut(s)	2251, 2656
Bst2BI	CACGAG	2 cut(s)	564, 2648
Bst2UI	CCWGG	7 cut(s)	755, 963, 1651, 2383, 2597, 2695, 2763
Bst6I	CTCTTC	6 cut(s)	288, 501, 519, 1579, 2471, 2769
BstACI	GRCGYC	1 cut(s)	18
BstAFI	CTTAAG	2 cut(s)	451, 1201
BstAPI	GCANNNNNTGC	1 cut(s)	1928
BstAUI	TGTACA	1 cut(s)	1216
BstBAI	YACGTR	1 cut(s)	650
BstC8I	GCNNGC	9 cut(s)	177, 246, 1141, 1370, 1656, 2154, 2266, 2455, 2511
BstEII	GGTNACC	1 cut(s)	1645
BstENI	CCTNNNNNAGG	2 cut(s)	1921, 2660
BstF5I	GGATG	6 cut(s)	60, 1736, 2378, 2432, 2494, 2587
BstFNI	CGCG	2 cut(s)	300, 1548
BstH2I	RGCGCY	1 cut(s)	1527
BstHHI	GCGC	2 cut(s)	300, 1526
BstMAI	GTCTC	5 cut(s)	95, 815, 1409, 2345, 2760
BstNI	CCWGG	7 cut(s)	755, 963, 1651, 2383, 2597, 2695, 2763
BstNSI	RCATGY	4 cut(s)	368, 1357, 1372, 1422
BstPI	GGTNACC	1 cut(s)	1645
BstSCI	CCNGG	8 cut(s)	7, 753, 961, 1649, 2381, 2595, 2693, 2761
BstSFI	CTRYAG	3 cut(s)	1062, 1743, 1833
BstSLI	GKGCMC	2 cut(s)	328, 1060
BstUI	CGCG	2 cut(s)	300, 1548
BstV1I	GCAGC	7 cut(s)	1091, 1159, 1244, 1417, 2044, 2297, 2715
BstV2I	GAAGAC	3 cut(s)	88, 135, 2553
BstX2I	RGATCY	4 cut(s)	1955, 1987, 2026, 2365
BstXI	CCANNNNNNTGG	3 cut(s)	1859, 2389, 2701
BstYI	RGATCY	4 cut(s)	1955, 1987, 2026, 2365
BsuI	GTATCC	1 cut(s)	1738
BsuRI	GGCC	6 cut(s)	623, 1162, 1278, 1565, 2256, 2513
BtgZI	GCGATG	1 cut(s)	1529
BtrI	CACGTC	3 cut(s)	1697, 2281, 2719
BtsCI	GGATG	6 cut(s)	60, 1736, 2378, 2432, 2494, 2587
BtsI	GCAGTG	2 cut(s)	2151, 2234
BtsIMutI	CAGTG	7 cut(s)	408, 1058, 1441, 1475, 1752, 2151, 2234
BveI	ACCTGC	1 cut(s)	403
Cac8I	GCNNGC	9 cut(s)	177, 246, 1141, 1370, 1656, 2154, 2266, 2455, 2511
CaiI	CAGNNNCTG	2 cut(s)	956, 2291
CciI	TCATGA	1 cut(s)	1452
CfoI	GCGC	2 cut(s)	300, 1526
Cfr10I	RCCGGY	1 cut(s)	2032
Cfr13I	GGNCC	4 cut(s)	1021, 1160, 1564, 2245
CseI	GACGC	1 cut(s)	7
Csp6I	GTAC	2 cut(s)	482, 1217
CspAI	ACCGGT	1 cut(s)	2032
CviQI	GTAC	2 cut(s)	482, 1217
EaeI	YGGCCR	2 cut(s)	1276, 2254
Eam1104I	CTCTTC	6 cut(s)	288, 501, 519, 1579, 2471, 2769
EarI	CTCTTC	6 cut(s)	288, 501, 519, 1579, 2471, 2769
Ecl136II	GAGCTC	1 cut(s)	231
Eco130I	CCWWGG	2 cut(s)	2251, 2656
Eco147I	AGGCCT	1 cut(s)	623
Eco24I	GRGCYC	3 cut(s)	233, 1938, 2596
Eco31I	GGTCTC	1 cut(s)	95
Eco32I	GATATC	1 cut(s)	2740
Eco47I	GGWCC	2 cut(s)	1021, 2245
Eco53kI	GAGCTC	1 cut(s)	231
Eco57I	CTGAAG	5 cut(s)	194, 1901, 2004, 2495, 2547
Eco72I	CACGTG	1 cut(s)	650
Eco91I	GGTNACC	1 cut(s)	1645
EcoICRI	GAGCTC	1 cut(s)	231
EcoNI	CCTNNNNNAGG	2 cut(s)	1921, 2660
EcoO109I	RGGNCCY	1 cut(s)	2245
EcoO65I	GGTNACC	1 cut(s)	1645
EcoRII	CCWGG	7 cut(s)	753, 961, 1649, 2381, 2595, 2693, 2761
EcoRV	GATATC	1 cut(s)	2740
EcoT14I	CCWWGG	2 cut(s)	2251, 2656
EcoT22I	ATGCAT	4 cut(s)	799, 1094, 1545, 1615
EcoT38I	GRGCYC	3 cut(s)	233, 1938, 2596
ErhI	CCWWGG	2 cut(s)	2251, 2656
Esp3I	CGTCTC	1 cut(s)	2760
FalI	AAGNNNNNCTT	2 cut(s)	1395, 1427
FaqI	GGGAC	1 cut(s)	1683
FauNDI	CATATG	1 cut(s)	1615
FbaI	TGATCA	1 cut(s)	2011
FokI	GGATG	6 cut(s)	67, 1723, 2365, 2419, 2501, 2594
FriOI	GRGCYC	3 cut(s)	233, 1938, 2596
FspBI	CTAG	5 cut(s)	1212, 1655, 1668, 1688, 2525
GlaI	GCGC	2 cut(s)	299, 1525
GsaI	CCCAGC	1 cut(s)	1040
GsuI	CTGGAG	1 cut(s)	2784
HaeII	RGCGCY	1 cut(s)	1527
HaeIII	GGCC	6 cut(s)	623, 1162, 1278, 1565, 2256, 2513
HapII	CCGG	2 cut(s)	8, 2033
HgaI	GACGC	1 cut(s)	7
HhaI	GCGC	2 cut(s)	300, 1526
Hin1I	GRCGYC	1 cut(s)	18
Hin6I	GCGC	2 cut(s)	298, 1524
HinP1I	GCGC	2 cut(s)	298, 1524
HincII	GTYRAC	1 cut(s)	1943
HindII	GTYRAC	1 cut(s)	1943
HindIII	AAGCTT	2 cut(s)	2266, 2684
HpaII	CCGG	2 cut(s)	8, 2033
HphI	GGTGA	6 cut(s)	13, 76, 1657, 1820, 1906, 2783
Hpy166II	GTNNAC	7 cut(s)	1217, 1351, 1830, 1943, 2068, 2119, 2722
Hpy8I	GTNNAC	7 cut(s)	1217, 1351, 1830, 1943, 2068, 2119, 2722
Hpy99I	CGWCG	1 cut(s)	305
HpyAV	CCTTC	5 cut(s)	680, 719, 1450, 1849, 2611
HpyCH4IV	ACGT	4 cut(s)	649, 1696, 2280, 2718
HpySE526I	ACGT	4 cut(s)	649, 1696, 2280, 2718
Hsp92I	GRCGYC	1 cut(s)	18
HspAI	GCGC	2 cut(s)	298, 1524
Ksp22I	TGATCA	1 cut(s)	2011
LguI	GCTCTTC	2 cut(s)	288, 519
LmnI	GCTCC	2 cut(s)	205, 254
Lsp1109I	GCAGC	7 cut(s)	1091, 1159, 1244, 1417, 2044, 2297, 2715
MaeI	CTAG	5 cut(s)	1212, 1655, 1668, 1688, 2525
MaeII	ACGT	4 cut(s)	649, 1696, 2280, 2718
MaeIII	GTNAC	6 cut(s)	19, 71, 537, 858, 1374, 1645
MfeI	CAATTG	1 cut(s)	825
MflI	RGATCY	4 cut(s)	1955, 1987, 2026, 2365
MhlI	GDGCHC	7 cut(s)	233, 241, 328, 1060, 1938, 2234, 2596
MlsI	TGGCCA	1 cut(s)	1278
MluNI	TGGCCA	1 cut(s)	1278
MlyI	GAGTC	4 cut(s)	346, 1318, 1857, 2488
MmeI	TCCRAC	6 cut(s)	897, 1840, 2370, 2493, 2545, 2682
Mox20I	TGGCCA	1 cut(s)	1278
Mph1103I	ATGCAT	4 cut(s)	799, 1094, 1545, 1615
MroXI	GAANNNNTTC	3 cut(s)	315, 549, 2305
MscI	TGGCCA	1 cut(s)	1278
MslI	CAYNNNNRTG	1 cut(s)	1087
Msp20I	TGGCCA	1 cut(s)	1278
MspA1I	CMGCKG	2 cut(s)	244, 1235
MspCI	CTTAAG	2 cut(s)	451, 1201
MspI	CCGG	2 cut(s)	8, 2033
MspR9I	CCNGG	8 cut(s)	9, 755, 963, 1651, 2383, 2597, 2695, 2763
MunI	CAATTG	1 cut(s)	825
Mva1269I	GAATGC	1 cut(s)	799
MvaI	CCWGG	7 cut(s)	755, 963, 1651, 2383, 2597, 2695, 2763
MvnI	CGCG	2 cut(s)	300, 1548
NciI	CCSGG	1 cut(s)	9
NdeI	CATATG	1 cut(s)	1615
NheI	GCTAGC	1 cut(s)	1654
NlaIV	GGNNCC	5 cut(s)	325, 1059, 1161, 1506, 2151
NmuCI	GTSAC	2 cut(s)	19, 1645
NsiI	ATGCAT	4 cut(s)	799, 1094, 1545, 1615
NspI	RCATGY	4 cut(s)	368, 1357, 1372, 1422
PaeI	GCATGC	1 cut(s)	1372
PagI	TCATGA	1 cut(s)	1452
PceI	AGGCCT	1 cut(s)	623
PciI	ACATGT	2 cut(s)	1353, 1418
PciSI	GCTCTTC	2 cut(s)	288, 519
PctI	GAATGC	1 cut(s)	799
PdmI	GAANNNNTTC	3 cut(s)	315, 549, 2305
PfeI	GAWTC	8 cut(s)	388, 1123, 1194, 1303, 1691, 1841, 2423, 2465
PfoI	TCCNGGA	2 cut(s)	753, 961
PinAI	ACCGGT	1 cut(s)	2032
PleI	GAGTC	4 cut(s)	346, 1318, 1857, 2487
PmaCI	CACGTG	1 cut(s)	650
PmlI	CACGTG	1 cut(s)	650
PpsI	GAGTC	4 cut(s)	346, 1318, 1857, 2487
Ppu21I	YACGTR	1 cut(s)	650
PpuMI	RGGWCCY	1 cut(s)	2245
PscI	ACATGT	2 cut(s)	1353, 1418
PshBI	ATTAAT	3 cut(s)	842, 900, 927
Psp124BI	GAGCTC	1 cut(s)	233
Psp5II	RGGWCCY	1 cut(s)	2245
Psp6I	CCWGG	7 cut(s)	753, 961, 1649, 2381, 2595, 2693, 2761
PspCI	CACGTG	1 cut(s)	650
PspEI	GGTNACC	1 cut(s)	1645
PspFI	CCCAGC	1 cut(s)	1036
PspGI	CCWGG	7 cut(s)	753, 961, 1649, 2381, 2595, 2693, 2761
PspN4I	GGNNCC	5 cut(s)	325, 1059, 1161, 1506, 2151
PspPI	GGNCC	4 cut(s)	1021, 1160, 1564, 2245
PspPPI	RGGWCCY	1 cut(s)	2245
PstNI	CAGNNNCTG	2 cut(s)	956, 2291
PsuI	RGATCY	4 cut(s)	1955, 1987, 2026, 2365
PvuII	CAGCTG	2 cut(s)	244, 1235
RsaI	GTAC	2 cut(s)	483, 1218
RsaNI	GTAC	2 cut(s)	482, 1217
RseI	CAYNNNNRTG	1 cut(s)	1087
SacI	GAGCTC	1 cut(s)	233
SapI	GCTCTTC	2 cut(s)	288, 519
Sau96I	GGNCC	4 cut(s)	1021, 1160, 1564, 2245
SchI	GAGTC	4 cut(s)	346, 1318, 1857, 2488
ScrFI	CCNGG	8 cut(s)	9, 755, 963, 1651, 2383, 2597, 2695, 2763
SduI	GDGCHC	7 cut(s)	233, 241, 328, 1060, 1938, 2234, 2596
SfcI	CTRYAG	3 cut(s)	1062, 1743, 1833
SinI	GGWCC	2 cut(s)	1021, 2245
SmiMI	CAYNNNNRTG	1 cut(s)	1087
SmlI	CTYRAG	7 cut(s)	451, 1201, 1337, 1412, 1499, 2399, 2504
SmoI	CTYRAG	7 cut(s)	451, 1201, 1337, 1412, 1499, 2399, 2504
SphI	GCATGC	1 cut(s)	1372
SseBI	AGGCCT	1 cut(s)	623
SsiI	CCGC	5 cut(s)	1740, 1766, 2203, 2257, 2386
SspMI	CTAG	5 cut(s)	1212, 1655, 1668, 1688, 2525
SstI	GAGCTC	1 cut(s)	233
StuI	AGGCCT	1 cut(s)	623
StyD4I	CCNGG	8 cut(s)	7, 753, 961, 1649, 2381, 2595, 2693, 2761
StyI	CCWWGG	2 cut(s)	2251, 2656
TaiI	ACGT	4 cut(s)	652, 1699, 2283, 2721
TaqI	TCGA	3 cut(s)	319, 1407, 2679
TaqII	GACCGA	1 cut(s)	2301
TatI	WGTACW	1 cut(s)	1216
TauI	GCSGC	3 cut(s)	1769, 2206, 2259
TfiI	GAWTC	8 cut(s)	388, 1123, 1194, 1303, 1691, 1841, 2423, 2465
TscAI	CASTG	7 cut(s)	415, 1058, 1441, 1475, 1752, 2151, 2234
TseFI	GTSAC	2 cut(s)	19, 1645
TseI	GCWGC	7 cut(s)	1079, 1172, 1232, 1430, 2057, 2285, 2728
Tsp45I	GTSAC	2 cut(s)	19, 1645
TspDTI	ATGAA	9 cut(s)	17, 141, 419, 1469, 1595, 1667, 1725, 1905, 2333
TspGWI	ACGGA	2 cut(s)	205, 1061
TspRI	CASTG	7 cut(s)	415, 1058, 1441, 1475, 1752, 2151, 2234
Vha464I	CTTAAG	2 cut(s)	451, 1201
VpaK11BI	GGWCC	2 cut(s)	1021, 2245
VspI	ATTAAT	3 cut(s)	842, 900, 927
XagI	CCTNNNNNAGG	2 cut(s)	1921, 2660
XapI	RAATTY	5 cut(s)	523, 663, 1223, 2213, 2446
XceI	RCATGY	4 cut(s)	368, 1357, 1372, 1422
XmnI	GAANNNNTTC	3 cut(s)	315, 549, 2305
XspI	CTAG	5 cut(s)	1212, 1655, 1668, 1688, 2525
Zsp2I	ATGCAT	4 cut(s)	799, 1094, 1545, 1615

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.