Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004196

Physical Location & Seq

Genomic Coordinates Reverse (-)

1665664 .. 1671731

Gene Structure 6068 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1022800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2727 bp

ATGGATTTGGTCTCTGGTTACAAGGGAGTTGTGGGGCTTGTTTTCGGCAACGAGAAATCGGGTTCTTCGAATGAAGATAGCTATGTCGAACGTTTGCTCGACTGTATAAGCAATGGTAAACTGGCAGAGGACAGGAGGACCGCTATGATCGAGCTTCAGTCTGTTGTGGCAGAAAGCTCTGCTGCCCAACTAGCATTTGGAGCAATGGGATTTCCTGTTATCATGGGCATATTAAAGGAAGAACGGGATGATGTTGAAATGGTCCGAGGTGCCCTAGAAACTCTTGTTAGTGCCTTGACTCCTATTGACCATGCAAAAGGCCCAAAGAATGAGATTCAACCAGCTATAATGAATACTGATTTGCTTTCCAGGGAAGCAGACAGCATTTCGCTTCTTCTAAGTTTGTTGTCAGAGGAGGATTTCTATGTACGATATTACACTCTTCAACTCTTGACAGCTCTTCTCACAAATTCTCGAAATAGGTTACAGGAAGCCATTCTGACCATACCTCGTGGTATAACTCGTTTAATGGATATGCTTATGGATCGTGAGGTTATACGAAATGAGGCCTTATTACTTCTTACTTACTTGACTCGCGAAGCTGAGGAAATTCAAAAAATTGTGGTCTTTGAAGGTGCTTTTGAAAAGATTTTCAGCATCATCAAAGAGGAAGGTGGTTCTGATGGTGGTGTTGTTGTGCAGGACTGTATTGAATTGTTGAACAATCTTATTCGGAACACTGCATCTAATCAGATACTACTGAGGGAGACAATGGGATTTGATCCCTTAATGTCAATTTTGAAGCTACGAGGGAGTACTTACAGTTTCACTCAACAAAAGACAATTAATCTACTCAGTGCATTGGAAACTCTTAATTTGCTAATATTGGGAGGGTCGGAGGCTGATCCTGGAAAAGATGCAAATAAGCTAACAAATAGAACCACCCTCGTTCAGAAAAAAGTCTTGGACCATCTTCTAATGCTGGGTGTGGAAAGCCAGTGGGCTCCTGTTGCTGTCCGTTGTTCAGCGCTGCAATGCATTGGTAATCTGATTGCTGGACATCCCAAGAATCTTGATGCGCTTGCAAGCAAATTTCTTGGAGAGGGGCCACAGGAACCTGCTTTGAACTCAATTCTTCGAATCATATTGCGGACATCCAGTATGCAAGAATTTGTTGCAGCAGATTATGTTTTTAAGAGATTTTGTGAGAATAATGTCGATGGCCAAACTATGTTAGCGTCAACATTGATTCCACAACCGCATTCCATGATTCATGCACCCGTTGAGGATGATGTGAACATGTCTTTTGGAAGCATGCTATTACAAGGTCTTAACTTCAGTGAGAAAGATGGTGATCTTGAGACATGCTGCAGAGCTGCTAGTGTTCTTTCTCATGTAATGAAGGACAATCTCCAGTGCAAGGAAAGGGTGTTGCGTATTGAACTTGAGGCACCTACTCCATCTCTAGGTGCACCAGAGCCTCTAATGCACCGTGTGGTAAAATACTTAGCTGTTACCTCTTCCATGCAAAATAAAGATGGAAAGTCAAGCAGAAACTCATATGTTCAACCAGTTATCTTGAAATTGCTGGTCACTTGGCTGGCCGATTGCCCTAGCGCTGTGAATTGCTTCCTAGATTCACGTCCCCACATCACTTATCTGCTTGAGTTGGTCTCCAATTCAACTGCTACAGTGTACATAAAGGGGTTGGCAGCTGTTCTTCTGGGAGAGTGTGTGATCTACAACAATTCAGTTGAAAGTGAGAAGGACGCCTTTACTATAGTTGATTCCATAAGCCAGAAGGTTGGGCTCACGTCATATTTTCTGAAGTTCGATGAGATGCAGAAAAGCTTCCTTTTCACCTCTTCAAAATCGGCTCAGCCTCGCAAAGAATTAACCAGATCCGCTTCTGCTAGTATGGTAGATGTTGATGAGAGTGATCTCTCTGATCAGAAAACTGAGGATCATCCGGTTCTCTCCTCAATCTTTGATGCTCCATTTCTAAACCTAGTTAAGAGTTTGGAGGCGAACATCAGAGAAAAAATCGTTGAGGTGTACAGTCAACCGAAGAGCAAGGTGGCAGTGGTGCCAGCAGAACTGGAACAGAAAAGTGGGGAAAGTGATGGAGAGTATATACAGCGGCTGAAAGCATTTGTGGAAAAGCAATGCTCGGAGATACAGGACCTCCTTGGCCGCAATGCAAGTTTAGCTGAGGATGTGGCAACGATTGGTGGGGGAAGTTCCAGGTCTGAGCAGGGTGCAGGCTCAGATAGAGTTCAGGTAGAGACGCTTAAACGAGATTTTCAAGAAGCATCTAAACGGTTGGAGTTGCTCAAGGCAGAGAAAGCCAAGCTTGAGTCCGAGGCATCCATGTATAAAAACTTAGCCGGAAAGATGGAATCTGATCTAAAGAGCTTATCCGATGCTTACAACAGTCTTGAGCAGGCGAATTTCCATCTAGAGAAGGAGGTCAGAGCTAAGAATGGTGGAGGAGGGTCTTCGTCCATTCCCGATGTGGATGCAATTAGAGCCGAGGCAAGGGAAGAAGCTCAGAAGGAGAGCGAGGCAGAACTGAATGATCTCCTCGTGTGCCTCGGACAAGAACAGAGCAAGGTGGAAAAACTAAGTGCAAGGTTGTTGGAGTTAGGAGAGGACGTCGACAAGCTGCTTGAAGATATCGGAGATGACGTGGGGTTGCCCGGAGACGGCGAGGAGGATGAAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005795 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0007275 GO:0008104 GO:0008150 GO:0008565 GO:0009791 GO:0009987 GO:0012505 GO:0012506 GO:0012507 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0022406 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032527 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045056 GO:0045184 GO:0046907 GO:0048193 GO:0048211 GO:0048278 GO:0048856 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0061024 GO:0061025 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0140056

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Uso1_p115_C Uso1_p115_head

Protein Analysis

909

Amino Acids

99.78

Weight (kDa)

4.8

Isoelectric Point (pI)

39.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Uso1_p115_head	PF04869	373 - 681	6.1e-24	Uso1 / p115 like vesicle tethering protein, head region
Uso1_p115_C	PF04871	781 - 906	8.6e-29	Uso1 / p115 like vesicle tethering protein, C terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004196)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27530
fragaria_vesca	FvH4_1g06690 FvH4_1g06690 FvH4_6g51710 FvH4_6g51710 FvH4_6g51710 FvH4_6g51710
malus_domestica	MD09G1023300.v1.1 MD17G1022800.v1.1
prunus_persica	Prupe.3G293500_v2.0.a1 Prupe.3G293600_v2.0.a1
pyrus_communis	pycom111g01940
rosa_chinensis	RchiOBHm_Chr2g0173241 RchiOBHm_Chr2g0173251
rosa_laevigata	RLG00000022173 RLG00000022175
rosa_multiflora	Rmu_sc0010961.1_g000008 Rmu_sc0010961.1_g000011
rosa_roxburghii	Rroxscaffold_2G00079090
rosa_rugosa	Rorug02G0566800 Rorug02G0566900 Rorug02G0566900 Rorug02G0566900
rosa_samantha	Rh2AG646700 Rh2BG657400 Rh2CG622600 Rh2CG623100 Rh2CG623200 Rh2DG671000 Rh2DG671500 Rh2DG671600
rosa_wichuraiana	Rw2G053120 Rw2G053130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2658
Acc36I	ACCTGC	1 cut(s)	1126
AccB1I	GGYRCC	3 cut(s)	269, 1450, 2086
AccI	GTMKAC	1 cut(s)	2658
AccII	CGCG	1 cut(s)	597
AciI	CCGC	6 cut(s)	141, 1150, 1259, 1905, 2140, 2194
AclI	AACGTT	1 cut(s)	91
AclWI	GGATC	5 cut(s)	552, 776, 899, 1896, 1971
AcoI	YGGCCR	3 cut(s)	1222, 1602, 2191
AcsI	RAATTY	5 cut(s)	469, 609, 1091, 1169, 2449
AcuI	CTGAAG	3 cut(s)	140, 1321, 1847
AcyI	GRCGYC	2 cut(s)	1770, 2655
AdeI	CACNNNGTG	1 cut(s)	1495
AfaI	GTAC	4 cut(s)	429, 817, 1697, 2057
AfeI	AGCGCT	2 cut(s)	1029, 1618
AfiI	CCNNNNNNNGG	4 cut(s)	1420, 1466, 2538, 2705
AflIII	ACRYGT	1 cut(s)	1299
AjiI	CACGTC	3 cut(s)	1643, 1815, 2689
AjnI	CCWGG	3 cut(s)	368, 907, 2243
AjuI	GAANNNNNNNTTGG	2 cut(s)	947, 979
Alw21I	GWGCWC	1 cut(s)	1474
Alw26I	GTCTC	6 cut(s)	16, 761, 1355, 1678, 2279, 2697
Alw44I	GTGCAC	1 cut(s)	1470
AlwI	GGATC	5 cut(s)	552, 776, 899, 1896, 1971
AlwNI	CAGNNNCTG	1 cut(s)	2143
Aor51HI	AGCGCT	2 cut(s)	1029, 1618
AoxI	GGCC	6 cut(s)	319, 567, 1106, 1222, 1602, 2191
ApaLI	GTGCAC	1 cut(s)	1470
ApeKI	GCWGC	7 cut(s)	182, 1030, 1178, 1368, 1376, 1712, 2665
ApoI	RAATTY	5 cut(s)	469, 609, 1091, 1169, 2449
AseI	ATTAAT	1 cut(s)	846
Asp700I	GAANNNNTTC	3 cut(s)	495, 1628, 1850
AspLEI	GCGC	3 cut(s)	1030, 1081, 1619
AspS9I	GGNCC	6 cut(s)	138, 262, 320, 967, 1106, 2182
AsuC2I	CCSGG	1 cut(s)	2700
AsuHPI	GGTGA	2 cut(s)	1364, 1852
AsuII	TTCGAA	2 cut(s)	68, 1138
AvaII	GGWCC	4 cut(s)	138, 262, 967, 2182
BaeGI	GKGCMC	2 cut(s)	274, 1474
BalI	TGGCCA	1 cut(s)	1224
BanI	GGYRCC	3 cut(s)	269, 1450, 2086
BanII	GRGCYC	2 cut(s)	1006, 1812
BauI	CACGAG	2 cut(s)	510, 2585
BbsI	GAAGAC	1 cut(s)	2490
Bbv12I	GWGCWC	1 cut(s)	1474
BbvCI	CCTCAGC	2 cut(s)	603, 2211
BbvI	GCAGC	7 cut(s)	169, 1017, 1190, 1355, 1363, 1724, 2652
BccI	CCATC	9 cut(s)	677, 978, 1214, 1343, 1468, 1532, 2117, 2389, 2463
BceAI	ACGGC	1 cut(s)	2722
BcgI	CGANNNNNNTGC	2 cut(s)	2501, 2535
BciT130I	CCWGG	3 cut(s)	370, 909, 2245
BclI	TGATCA	1 cut(s)	1948
BcnI	CCSGG	1 cut(s)	2700
BcoDI	GTCTC	6 cut(s)	16, 761, 1355, 1678, 2279, 2697
BfaI	CTAG	9 cut(s)	191, 275, 1380, 1466, 1614, 1634, 1914, 2009, 2459
BfmI	CTRYAG	3 cut(s)	1369, 1689, 1779
BfoI	RGCGCY	2 cut(s)	1031, 1620
BfuAI	ACCTGC	1 cut(s)	1126
BisI	GCNGC	9 cut(s)	183, 1031, 1179, 1369, 1377, 1713, 2141, 2194, 2666
BlpI	GCTNAGC	1 cut(s)	1878
BlsI	GCNGC	9 cut(s)	184, 1032, 1180, 1370, 1378, 1714, 2142, 2195, 2667
BmcAI	AGTACT	1 cut(s)	817
Bme1390I	CCNGG	4 cut(s)	370, 909, 2245, 2700
Bme18I	GGWCC	4 cut(s)	138, 262, 967, 2182
BmgBI	CACGTC	3 cut(s)	1643, 1815, 2689
BmgT120I	GGNCC	6 cut(s)	138, 262, 320, 967, 1106, 2182
BmiI	GGNNCC	6 cut(s)	271, 1005, 1107, 1116, 1452, 2088
BmrFI	CCNGG	4 cut(s)	370, 909, 2245, 2700
BpiI	GAAGAC	1 cut(s)	2490
BplI	GAGNNNNNCTC	2 cut(s)	1658, 1690
BpmI	CTGGAG	1 cut(s)	1397
Bpu10I	CCTNAGC	2 cut(s)	603, 2211
Bpu1102I	GCTNAGC	1 cut(s)	1878
Bpu14I	TTCGAA	2 cut(s)	68, 1138
BpuEI	CTTGAG	6 cut(s)	1379, 1466, 1685, 2318, 2375, 2459
BpuMI	CCSGG	1 cut(s)	2700
BsaBI	GATNNNNATC	1 cut(s)	1353
BsaHI	GRCGYC	2 cut(s)	1770, 2655
BsaI	GGTCTC	2 cut(s)	16, 1678
BsaJI	CCNNGG	6 cut(s)	265, 369, 2188, 2361, 2532, 2593
BsaWI	WCCGGW	1 cut(s)	1969
BsaXI	ACNNNNNCTCC	8 cut(s)	1719, 1749, 2169, 2199, 2640, 2670, 2673, 2703
Bsc4I	CCNNNNNNNGG	4 cut(s)	1420, 1466, 2538, 2705
Bse1I	ACTGG	6 cut(s)	126, 997, 1158, 1414, 1571, 2103
Bse3DI	GCAATG	5 cut(s)	118, 210, 1040, 2171, 2203
Bse8I	GATNNNNATC	1 cut(s)	1353
BseBI	CCWGG	3 cut(s)	370, 909, 2245
BseDI	CCNNGG	6 cut(s)	265, 369, 2188, 2361, 2532, 2593
BseGI	GGATG	9 cut(s)	253, 1060, 1154, 1294, 1966, 2221, 2366, 2524, 2722
BseJI	GATNNNNATC	1 cut(s)	1353
BseLI	CCNNNNNNNGG	4 cut(s)	1420, 1466, 2538, 2705
BseMI	GCAATG	5 cut(s)	118, 210, 1040, 2171, 2203
BseMII	CTCAG	9 cut(s)	594, 752, 868, 1892, 1950, 2202, 2241, 2280, 2564
BseNI	ACTGG	6 cut(s)	126, 997, 1158, 1414, 1571, 2103
BseRI	GAGGAG	5 cut(s)	428, 1969, 2505, 2573, 2726
BseSI	GKGCMC	2 cut(s)	274, 1474
BseXI	GCAGC	7 cut(s)	169, 1017, 1190, 1355, 1363, 1724, 2652
BseYI	CCCAGC	1 cut(s)	982
BsgI	GTGCAG	2 cut(s)	719, 2280
Bsh1236I	CGCG	1 cut(s)	597
BshFI	GGCC	6 cut(s)	321, 569, 1108, 1224, 1604, 2193
BshNI	GGYRCC	3 cut(s)	269, 1450, 2086
BsiHKAI	GWGCWC	1 cut(s)	1474
BsiSI	CCGG	3 cut(s)	1970, 2388, 2700
BslFI	GGGAC	1 cut(s)	1629
BslI	CCNNNNNNNGG	4 cut(s)	1420, 1466, 2538, 2705
BsmAI	GTCTC	6 cut(s)	16, 761, 1355, 1678, 2279, 2697
BsmBI	CGTCTC	2 cut(s)	2279, 2697
BsmFI	GGGAC	1 cut(s)	1629
BsmI	GAATGC	1 cut(s)	1261
BsnI	GGCC	6 cut(s)	321, 569, 1108, 1224, 1604, 2193
Bso31I	GGTCTC	2 cut(s)	16, 1678
Bsp119I	TTCGAA	2 cut(s)	68, 1138
Bsp1286I	GDGCHC	4 cut(s)	274, 1006, 1474, 1812
Bsp1407I	TGTACA	2 cut(s)	1695, 2055
Bsp1720I	GCTNAGC	1 cut(s)	1878
Bsp68I	TCGCGA	1 cut(s)	597
BspACI	CCGC	6 cut(s)	141, 1150, 1259, 1905, 2140, 2194
BspANI	GGCC	6 cut(s)	321, 569, 1108, 1224, 1604, 2193
BspCNI	CTCAG	9 cut(s)	595, 753, 867, 1891, 1951, 2203, 2242, 2279, 2563
BspFNI	CGCG	1 cut(s)	597
BspLI	GGNNCC	6 cut(s)	271, 1005, 1107, 1116, 1452, 2088
BspMAI	CTGCAG	1 cut(s)	1373
BspMI	ACCTGC	1 cut(s)	1126
BspPI	GGATC	5 cut(s)	552, 776, 899, 1896, 1971
BspQI	GCTCTTC	2 cut(s)	465, 2063
BspT104I	TTCGAA	2 cut(s)	68, 1138
BspT107I	GGYRCC	3 cut(s)	269, 1450, 2086
BspTNI	GGTCTC	2 cut(s)	16, 1678
BsrDI	GCAATG	5 cut(s)	118, 210, 1040, 2171, 2203
BsrGI	TGTACA	2 cut(s)	1695, 2055
BsrI	ACTGG	6 cut(s)	126, 997, 1158, 1414, 1571, 2103
BssECI	CCNNGG	6 cut(s)	265, 369, 2188, 2361, 2532, 2593
BssNI	GRCGYC	2 cut(s)	1770, 2655
BssSI	CACGAG	2 cut(s)	510, 2585
BssT1I	CCWWGG	1 cut(s)	2188
Bst2BI	CACGAG	2 cut(s)	510, 2585
Bst2UI	CCWGG	3 cut(s)	370, 909, 2245
Bst4CI	ACNGT	8 cut(s)	104, 707, 824, 1493, 1693, 2060, 2322, 2435
Bst6I	CTCTTC	5 cut(s)	447, 465, 1525, 1870, 2063
BstACI	GRCGYC	2 cut(s)	1770, 2655
BstAUI	TGTACA	2 cut(s)	1695, 2055
BstBI	TTCGAA	2 cut(s)	68, 1138
BstC8I	GCNNGC	7 cut(s)	1083, 1087, 1316, 1602, 2091, 2263, 2445
BstF5I	GGATG	9 cut(s)	253, 1060, 1154, 1294, 1966, 2221, 2366, 2524, 2722
BstFNI	CGCG	1 cut(s)	597
BstH2I	RGCGCY	2 cut(s)	1031, 1620
BstHHI	GCGC	3 cut(s)	1030, 1081, 1619
BstMAI	GTCTC	6 cut(s)	16, 761, 1355, 1678, 2279, 2697
BstMWI	GCNNNNNNNGC	7 cut(s)	191, 200, 1010, 1486, 2207, 2345, 2528
BstNI	CCWGG	3 cut(s)	370, 909, 2245
BstNSI	RCATGY	3 cut(s)	1303, 1318, 1368
BstSCI	CCNGG	4 cut(s)	368, 907, 2243, 2698
BstSFI	CTRYAG	3 cut(s)	1369, 1689, 1779
BstSLI	GKGCMC	2 cut(s)	274, 1474
BstUI	CGCG	1 cut(s)	597
BstV1I	GCAGC	7 cut(s)	169, 1017, 1190, 1355, 1363, 1724, 2652
BstV2I	GAAGAC	1 cut(s)	2490
BstX2I	RGATCY	1 cut(s)	1901
BstXI	CCANNNNNNTGG	1 cut(s)	1805
BstYI	RGATCY	1 cut(s)	1901
BsuRI	GGCC	6 cut(s)	321, 569, 1108, 1224, 1604, 2193
BtrI	CACGTC	3 cut(s)	1643, 1815, 2689
BtsCI	GGATG	9 cut(s)	253, 1060, 1154, 1294, 1966, 2221, 2366, 2524, 2722
BtsI	GCAGTG	2 cut(s)	738, 2088
BtsIMutI	CAGTG	7 cut(s)	738, 862, 1004, 1345, 1421, 1698, 2088
BtuMI	TCGCGA	1 cut(s)	597
BveI	ACCTGC	1 cut(s)	1126
Cac8I	GCNNGC	7 cut(s)	1083, 1087, 1316, 1602, 2091, 2263, 2445
CaiI	CAGNNNCTG	1 cut(s)	2143
CfoI	GCGC	3 cut(s)	1030, 1081, 1619
Cfr13I	GGNCC	6 cut(s)	138, 262, 320, 967, 1106, 2182
CseI	GACGC	3 cut(s)	1227, 1778, 2296
Csp6I	GTAC	4 cut(s)	428, 816, 1696, 2056
CviQI	GTAC	4 cut(s)	428, 816, 1696, 2056
DraIII	CACNNNGTG	1 cut(s)	1495
EaeI	YGGCCR	3 cut(s)	1222, 1602, 2191
Eam1104I	CTCTTC	5 cut(s)	447, 465, 1525, 1870, 2063
EarI	CTCTTC	5 cut(s)	447, 465, 1525, 1870, 2063
Eco130I	CCWWGG	1 cut(s)	2188
Eco147I	AGGCCT	1 cut(s)	569
Eco24I	GRGCYC	2 cut(s)	1006, 1812
Eco31I	GGTCTC	2 cut(s)	16, 1678
Eco32I	GATATC	1 cut(s)	2677
Eco47I	GGWCC	4 cut(s)	138, 262, 967, 2182
Eco47III	AGCGCT	2 cut(s)	1029, 1618
Eco57I	CTGAAG	3 cut(s)	140, 1321, 1847
EcoO109I	RGGNCCY	1 cut(s)	2182
EcoRII	CCWGG	3 cut(s)	368, 907, 2243
EcoRV	GATATC	1 cut(s)	2677
EcoT14I	CCWWGG	1 cut(s)	2188
EcoT22I	ATGCAT	1 cut(s)	1040
EcoT38I	GRGCYC	2 cut(s)	1006, 1812
ErhI	CCWWGG	1 cut(s)	2188
Esp3I	CGTCTC	2 cut(s)	2279, 2697
FalI	AAGNNNNNCTT	4 cut(s)	1757, 1789, 2337, 2369
FaqI	GGGAC	1 cut(s)	1629
FauNDI	CATATG	1 cut(s)	1561
FbaI	TGATCA	1 cut(s)	1948
FblI	GTMKAC	1 cut(s)	2658
Fnu4HI	GCNGC	9 cut(s)	183, 1031, 1179, 1369, 1377, 1713, 2141, 2194, 2666
FokI	GGATG	8 cut(s)	260, 1047, 1141, 1301, 1953, 2228, 2353, 2531
FriOI	GRGCYC	2 cut(s)	1006, 1812
Fsp4HI	GCNGC	9 cut(s)	183, 1031, 1179, 1369, 1377, 1713, 2141, 2194, 2666
FspBI	CTAG	9 cut(s)	191, 275, 1380, 1466, 1614, 1634, 1914, 2009, 2459
GlaI	GCGC	3 cut(s)	1029, 1080, 1618
GluI	GCNGC	9 cut(s)	183, 1031, 1179, 1369, 1377, 1713, 2141, 2194, 2666
GsaI	CCCAGC	1 cut(s)	986
GsuI	CTGGAG	1 cut(s)	1397
HaeII	RGCGCY	2 cut(s)	1031, 1620
HaeIII	GGCC	6 cut(s)	321, 569, 1108, 1224, 1604, 2193
HapII	CCGG	3 cut(s)	1970, 2388, 2700
HgaI	GACGC	3 cut(s)	1227, 1778, 2296
HhaI	GCGC	3 cut(s)	1030, 1081, 1619
Hin1I	GRCGYC	2 cut(s)	1770, 2655
Hin6I	GCGC	3 cut(s)	1028, 1079, 1617
HinP1I	GCGC	3 cut(s)	1028, 1079, 1617
HincII	GTYRAC	3 cut(s)	1242, 2063, 2659
HindII	GTYRAC	3 cut(s)	1242, 2063, 2659
HindIII	AAGCTT	2 cut(s)	1849, 2351
HpaII	CCGG	3 cut(s)	1970, 2388, 2700
HphI	GGTGA	2 cut(s)	1364, 1852
Hpy166II	GTNNAC	8 cut(s)	119, 1242, 1297, 1472, 1696, 2056, 2063, 2659
Hpy8I	GTNNAC	8 cut(s)	119, 1242, 1297, 1472, 1696, 2056, 2063, 2659
Hpy99I	CGWCG	1 cut(s)	2660
HpyAV	CCTTC	7 cut(s)	626, 665, 1396, 1759, 1795, 2458, 2548
HpyCH4III	ACNGT	8 cut(s)	104, 707, 824, 1493, 1693, 2060, 2322, 2435
HpyCH4IV	ACGT	5 cut(s)	91, 1642, 1814, 2655, 2688
HpyF10VI	GCNNNNNNNGC	7 cut(s)	191, 200, 1010, 1486, 2207, 2345, 2528
HpySE526I	ACGT	5 cut(s)	91, 1642, 1814, 2655, 2688
Hsp92I	GRCGYC	2 cut(s)	1770, 2655
HspAI	GCGC	3 cut(s)	1028, 1079, 1617
Ksp22I	TGATCA	1 cut(s)	1948
LguI	GCTCTTC	2 cut(s)	465, 2063
LmnI	GCTCC	3 cut(s)	200, 1009, 1999
Lsp1109I	GCAGC	7 cut(s)	169, 1017, 1190, 1355, 1363, 1724, 2652
MaeI	CTAG	9 cut(s)	191, 275, 1380, 1466, 1614, 1634, 1914, 2009, 2459
MaeII	ACGT	5 cut(s)	91, 1642, 1814, 2655, 2688
MaeIII	GTNAC	4 cut(s)	17, 483, 1513, 1591
MflI	RGATCY	1 cut(s)	1901
MhlI	GDGCHC	4 cut(s)	274, 1006, 1474, 1812
MlsI	TGGCCA	1 cut(s)	1224
MluNI	TGGCCA	1 cut(s)	1224
MlyI	GAGTC	3 cut(s)	292, 586, 2366
MmeI	TCCRAC	3 cut(s)	876, 2304, 2619
Mox20I	TGGCCA	1 cut(s)	1224
Mph1103I	ATGCAT	1 cut(s)	1040
MroXI	GAANNNNTTC	3 cut(s)	495, 1628, 1850
MscI	TGGCCA	1 cut(s)	1224
Msp20I	TGGCCA	1 cut(s)	1224
MspA1I	CMGCKG	2 cut(s)	1715, 2140
MspI	CCGG	3 cut(s)	1970, 2388, 2700
MspR9I	CCNGG	4 cut(s)	370, 909, 2245, 2700
Mva1269I	GAATGC	1 cut(s)	1261
MvaI	CCWGG	3 cut(s)	370, 909, 2245
MvnI	CGCG	1 cut(s)	597
MwoI	GCNNNNNNNGC	7 cut(s)	191, 200, 1010, 1486, 2207, 2345, 2528
NciI	CCSGG	1 cut(s)	2700
NdeI	CATATG	1 cut(s)	1561
NlaIV	GGNNCC	6 cut(s)	271, 1005, 1107, 1116, 1452, 2088
NmeAIII	GCCGAG	1 cut(s)	2557
NmuCI	GTSAC	1 cut(s)	1591
NruI	TCGCGA	1 cut(s)	597
NsiI	ATGCAT	1 cut(s)	1040
NspI	RCATGY	3 cut(s)	1303, 1318, 1368
NspV	TTCGAA	2 cut(s)	68, 1138
PaeI	GCATGC	1 cut(s)	1318
PceI	AGGCCT	1 cut(s)	569
PciI	ACATGT	1 cut(s)	1299
PciSI	GCTCTTC	2 cut(s)	465, 2063
PcsI	WCGNNNNNNNCGW	2 cut(s)	65, 2685
PctI	GAATGC	1 cut(s)	1261
PdmI	GAANNNNTTC	3 cut(s)	495, 1628, 1850
PfeI	GAWTC	8 cut(s)	334, 1069, 1140, 1249, 1270, 1637, 1787, 2399
PfoI	TCCNGGA	1 cut(s)	907
PkrI	GCNGC	9 cut(s)	184, 1032, 1180, 1370, 1378, 1714, 2142, 2195, 2667
PleI	GAGTC	3 cut(s)	292, 586, 2365
PpsI	GAGTC	3 cut(s)	292, 586, 2365
PpuMI	RGGWCCY	1 cut(s)	2182
PscI	ACATGT	1 cut(s)	1299
PshBI	ATTAAT	1 cut(s)	846
Psp1406I	AACGTT	1 cut(s)	91
Psp5II	RGGWCCY	1 cut(s)	2182
Psp6I	CCWGG	3 cut(s)	368, 907, 2243
PspFI	CCCAGC	1 cut(s)	982
PspGI	CCWGG	3 cut(s)	368, 907, 2243
PspN4I	GGNNCC	6 cut(s)	271, 1005, 1107, 1116, 1452, 2088
PspPI	GGNCC	6 cut(s)	138, 262, 320, 967, 1106, 2182
PspPPI	RGGWCCY	1 cut(s)	2182
PstI	CTGCAG	1 cut(s)	1373
PstNI	CAGNNNCTG	1 cut(s)	2143
PsuI	RGATCY	1 cut(s)	1901
PvuII	CAGCTG	1 cut(s)	1715
RruI	TCGCGA	1 cut(s)	597
RsaI	GTAC	4 cut(s)	429, 817, 1697, 2057
RsaNI	GTAC	4 cut(s)	428, 816, 1696, 2056
SalI	GTCGAC	1 cut(s)	2657
SapI	GCTCTTC	2 cut(s)	465, 2063
SatI	GCNGC	9 cut(s)	183, 1031, 1179, 1369, 1377, 1713, 2141, 2194, 2666
Sau96I	GGNCC	6 cut(s)	138, 262, 320, 967, 1106, 2182
ScaI	AGTACT	1 cut(s)	817
SchI	GAGTC	3 cut(s)	292, 586, 2366
ScrFI	CCNGG	4 cut(s)	370, 909, 2245, 2700
SduI	GDGCHC	4 cut(s)	274, 1006, 1474, 1812
SfcI	CTRYAG	3 cut(s)	1369, 1689, 1779
SfuI	TTCGAA	2 cut(s)	68, 1138
SinI	GGWCC	4 cut(s)	138, 262, 967, 2182
SmlI	CTYRAG	6 cut(s)	1358, 1445, 1664, 2333, 2354, 2438
SmoI	CTYRAG	6 cut(s)	1358, 1445, 1664, 2333, 2354, 2438
SphI	GCATGC	1 cut(s)	1318
SseBI	AGGCCT	1 cut(s)	569
SsiI	CCGC	6 cut(s)	141, 1150, 1259, 1905, 2140, 2194
SspI	AATATT	1 cut(s)	885
SspMI	CTAG	9 cut(s)	191, 275, 1380, 1466, 1614, 1634, 1914, 2009, 2459
StuI	AGGCCT	1 cut(s)	569
StyD4I	CCNGG	4 cut(s)	368, 907, 2243, 2698
StyI	CCWWGG	1 cut(s)	2188
TaaI	ACNGT	8 cut(s)	104, 707, 824, 1493, 1693, 2060, 2322, 2435
TaiI	ACGT	5 cut(s)	94, 1645, 1817, 2658, 2691
TaqI	TCGA	9 cut(s)	68, 87, 99, 150, 475, 1138, 1218, 1833, 2658
TatI	WGTACW	3 cut(s)	815, 1695, 2055
TauI	GCSGC	2 cut(s)	2143, 2196
TfiI	GAWTC	8 cut(s)	334, 1069, 1140, 1249, 1270, 1637, 1787, 2399
TscAI	CASTG	7 cut(s)	745, 862, 1004, 1345, 1421, 1698, 2088
TseFI	GTSAC	1 cut(s)	1591
TseI	GCWGC	7 cut(s)	182, 1030, 1178, 1368, 1376, 1712, 2665
Tsp45I	GTSAC	1 cut(s)	1591
TspDTI	ATGAA	4 cut(s)	87, 365, 1262, 1415
TspGWI	ACGGA	1 cut(s)	1007
TspRI	CASTG	7 cut(s)	745, 862, 1004, 1345, 1421, 1698, 2088
VneI	GTGCAC	1 cut(s)	1470
VpaK11BI	GGWCC	4 cut(s)	138, 262, 967, 2182
VspI	ATTAAT	1 cut(s)	846
XapI	RAATTY	5 cut(s)	469, 609, 1091, 1169, 2449
XbaI	TCTAGA	1 cut(s)	2458
XceI	RCATGY	3 cut(s)	1303, 1318, 1368
XcmI	CCANNNNNNNNNTGG	2 cut(s)	194, 2512
XmiI	GTMKAC	1 cut(s)	2658
XmnI	GAANNNNTTC	3 cut(s)	495, 1628, 1850
XspI	CTAG	9 cut(s)	191, 275, 1380, 1466, 1614, 1634, 1914, 2009, 2459
ZraI	GACGTC	1 cut(s)	2656
ZrmI	AGTACT	1 cut(s)	817
Zsp2I	ATGCAT	1 cut(s)	1040

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.