Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0004196

Physical Location & Seq

Genomic Coordinates Forward (+)

81506098 .. 81511514

Gene Structure 5417 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022173

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2736 bp

ATGGATTTGGTGAATAGTTACAAGGGGGTTGTAGGTCTCGTCTTCGGTAATGAGAAATCTTCTTCTTCAAATGAAGATAGTTATGTTGAGCGATTGCTGGACTGTATAAGCAATGGGAAGCTGGCAGAGGACAGGAGGACTGCTATGTTGGAGCTTCAGTCTGTTGTGGCAGAGAGCTCTGGTGCTCAGCTGGCATTTGGAGCAATGGGCTTTCCTGTGATGATGGGTGTATTGAGAGAAGAGCGCGACGATGTTGAAATGGTCCGAGGTGCCCTAGAAACTCTTGTAGGTGCCTTGACTCCTATTGAACATGCAAAAGTCCCAAAGAATGAGATTCAACCAGCTCTAATGAACACTGATTTGCTTTCCAGAGAAGCAGACAGCATTTCACTTCTCTTAAGTTTGTTGTCAGAGGAGGATTTCTATGTGCGATATTACACTCTTCAACTTTTGACAGCTCTTCTCACAAATTCTCCAAATAGGTTACAGGAAGCCATTCTGACCATACCTCGTGGTATAACTCGCCTAATGGATATGCTTATGGACCGTGAGGTTATACGAAATGAGGCCTTATTACTTCTTACTTACTTGACACGTGAAGCTGAGGAAATTCAAAAAATTGTGGTCTTTGAAGGTGCTTTTGAAAAGATTTTCAGCATCATCAAAGAGGAAGGTGGCTCAGACGGTGGTGTTGTTGTCCAGGACTGTATCGAACTGTTAAACAACCTTATTCGGAAGAATGCATCTAATCAGATACTACTGAGGGAGACAATTGGATTTGACCCATTAATGTCAATTTTGAAATTACGAGGAAGTACTTACAGTTTCACTCAACAAAAGACAATTAATCTACTCAGTTCGTTGGAAACTATTAATTTGCTGATAATGGGAGGTTCAGAGGCTGATTCTGGAAAAGATGCAAATAAGCTGACAAACAAGACAACTCTTGTTCAGAAAAAAATCTTGGACCTTCTTCTAATGCTGGGTGTAGAAAGCCAGTGGGCACCTGCTGCTGTCCGTTGTGCAGCATTACAATGCATCGGTAATCTAATTATCGGACATTCCAAGAATCTTGATGCCCTTGCAAACAAAGTTCTCGGAGAGGGGCCACAGGAACCTGCTTTGAACTCAATTCTACGAATCATCTTAAGGACATCTAGCGTGCAAGAATTTGTTGCAGCTGATTATGTTTTTAAGAGCTTTTGTGAGAAAAATGCTGATGGCCAAACAATGTTAGCGTCAACATTGATTCCTCAACCACATTCAATGACTCATGCACCCCTTGAGGAAGATGTGAACATGTCATTTGGAAGCATGCTGTTACAAGGTCTTACCTTGAGTGAGAGTGAAGTTGATTTTGAGACTTGTTGCAGAGCTGCCAGTGTTCTATCTTATGTCATGAAGGACAATATCCAGTGCAAGGAAAAGGTGTTGCATATTGAACTTGAGGCACCCACACCATCTTTAGGCGCTTCTGAGCCTCTAATGCATCGCGTGGTAAAATACTTGGCCCTTTCCTCTTCCATGAAAAACAAAGATGGAAAGTCAAGTGGAAATGCATATATTCAACCAATCCTCTTGAAAATGCTGGTGACTTGGCTAGCTGATTGCCCTAGTGCTGTTCATTGCTTCCTAGATTCACGTCCCCACATCACATATCTGCTTGAACTGGTATCCAGTTCATCCGCTACAGTGTTCGTAAAGGGATTGGCGGCTGTTCTTCTGGGAGAGTGCATTATCTACAATAAATCAGGTGAAAGTGGGAAAGATGCTTTTACTGTAGTTGATTCCATAAGCCAGAAGGTTGGACTCACATCGTACTTTCTGAAGTTTGATGAAATGAGAAAAAACTTCCTTTTCACATCTGCAAGGTCTGCTGAGCCCCGCCAACAATTAACCAGATCTGCTTCTGCTGGTATGGTAAATCCCGAAGATGTTGACGAGAACAATTTATCTGATCAGAAAGATGAGGATGTACCGGTTCTCTCCTCAATCTTTGATGCTGCATTTGTAAACCTGGTTAAGAGTTTAGAGGCAAACATCAGAGAAAAAATCGTTGAGGTTTACAGTCATCCAAAGAGCAAGGTGGCAGTGGTGCCAGCAGAATTGGAACAAAAGAGTGGGGAAAGTGATGGGGAGTATGTTAAGCGGTTGAAAGAATTTGTGGAAAAACAATGCTCTGAAATACAGGACCTCCTCGTTCGCAATGCAAGCTTGGCTGAGGACGTGGCAGCAACTGGTGGGGGAAGTCATTCTCAGTCTGAGCGAGGCACAGGCTCAGATAGAGTTCATGTAGAGACACTTAGACGAGATCTTCAAGAAGCATCTAGGCGGTTGGAGTTGCTCAAGGCAGAGAAAGCCAAGATTGAATCTGAGGCATCCATGTATAAAAATTTAGCTGGCAAGATGGAATCCGACCTGAAGAGTCTATCGGATGCTTACAACAGTCTTGAGCAGGCCAATTTCCAACTAGAAAAGGAGGTAAGAGGTCAAAAGAGTGTGGGGTCTTCAGCCTCTCCAGATGTGGATGCAATTAGAGCTCAGGCAAAGGAAGAAGCTCAGAAGGAGAGCGAGGCAGAACTGAATGATCTTCTTGTGTGCCTTGGACAAGAACAGAGCAAGGTCGAAAAGCTTAGTGCCAGGTTGTTGGAGTTGGGGGAGGACGTGGACAAGCTGCTTGAAGATATCGGAGATGATATGGGATTGCCTGGAGATGGTGAAGAGGAGGAAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005795 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0007275 GO:0008104 GO:0008150 GO:0008565 GO:0009791 GO:0009987 GO:0012505 GO:0012506 GO:0012507 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0022406 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032527 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045056 GO:0045184 GO:0046907 GO:0048193 GO:0048211 GO:0048278 GO:0048856 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0061024 GO:0061025 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0140056

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Uso1_p115_C Uso1_p115_head

Protein Analysis

912

Amino Acids

100.04

Weight (kDa)

4.82

Isoelectric Point (pI)

44.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Uso1_p115_head	PF04869	373 - 684	1.7e-25	Uso1 / p115 like vesicle tethering protein, head region
Uso1_p115_C	PF04871	785 - 909	2.2e-28	Uso1 / p115 like vesicle tethering protein, C terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004196)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27530
fragaria_vesca	FvH4_1g06690 FvH4_1g06690 FvH4_6g51710 FvH4_6g51710 FvH4_6g51710 FvH4_6g51710
malus_domestica	MD09G1023300.v1.1 MD17G1022800.v1.1
prunus_persica	Prupe.3G293500_v2.0.a1 Prupe.3G293600_v2.0.a1
pyrus_communis	pycom111g01940
rosa_chinensis	RchiOBHm_Chr2g0173241 RchiOBHm_Chr2g0173251
rosa_laevigata	RLG00000022173 RLG00000022175
rosa_multiflora	Rmu_sc0010961.1_g000008 Rmu_sc0010961.1_g000011
rosa_roxburghii	Rroxscaffold_2G00079090
rosa_rugosa	Rorug02G0566800 Rorug02G0566900 Rorug02G0566900 Rorug02G0566900
rosa_samantha	Rh2AG646700 Rh2BG657400 Rh2CG622600 Rh2CG623100 Rh2CG623200 Rh2DG671000 Rh2DG671500 Rh2DG671600
rosa_wichuraiana	Rw2G053120 Rw2G053130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1015
Acc36I	ACCTGC	2 cut(s)	1015, 1126
AccB1I	GGYRCC	5 cut(s)	269, 290, 1003, 1450, 2095
AccII	CGCG	2 cut(s)	246, 1494
AciI	CCGC	5 cut(s)	1686, 1712, 1885, 2149, 2332
AcoI	YGGCCR	1 cut(s)	1222
AcsI	RAATTY	5 cut(s)	469, 609, 1169, 2159, 2392
AcuI	CTGAAG	4 cut(s)	140, 1847, 2441, 2493
AcvI	CACGTG	1 cut(s)	596
AfaI	GTAC	3 cut(s)	817, 1820, 1977
AfiI	CCNNNNNNNGG	3 cut(s)	1420, 1466, 2714
AflII	CTTAAG	2 cut(s)	397, 1147
AflIII	ACRYGT	2 cut(s)	593, 1299
AgeI	ACCGGT	1 cut(s)	1978
AjiI	CACGTC	3 cut(s)	1643, 2227, 2665
AjnI	CCWGG	4 cut(s)	699, 2016, 2639, 2707
AjuI	GAANNNNNNNTTGG	2 cut(s)	947, 979
Alw21I	GWGCWC	3 cut(s)	179, 187, 2542
Alw26I	GTCTC	4 cut(s)	41, 761, 1355, 2291
AlwNI	CAGNNNCTG	2 cut(s)	902, 2237
AoxI	GGCC	5 cut(s)	567, 1106, 1222, 1509, 2457
ApeKI	GCWGC	7 cut(s)	1010, 1025, 1178, 1376, 2003, 2231, 2674
ApoI	RAATTY	5 cut(s)	469, 609, 1169, 2159, 2392
AseI	ATTAAT	3 cut(s)	788, 846, 873
AsiGI	ACCGGT	1 cut(s)	1978
Asp700I	GAANNNNTTC	2 cut(s)	495, 2251
AspLEI	GCGC	2 cut(s)	246, 1472
AspS9I	GGNCC	6 cut(s)	262, 544, 967, 1106, 1510, 2191
AsuHPI	GGTGA	4 cut(s)	22, 1603, 1766, 2729
AsuNHI	GCTAGC	1 cut(s)	1600
AvaII	GGWCC	4 cut(s)	262, 544, 967, 2191
BaeGI	GKGCMC	2 cut(s)	274, 1006
BalI	TGGCCA	1 cut(s)	1224
BanI	GGYRCC	5 cut(s)	269, 290, 1003, 1450, 2095
BanII	GRGCYC	3 cut(s)	179, 1884, 2542
BauI	CACGAG	1 cut(s)	510
BbrPI	CACGTG	1 cut(s)	596
BbsI	GAAGAC	2 cut(s)	34, 2499
Bbv12I	GWGCWC	3 cut(s)	179, 187, 2542
BbvCI	CCTCAGC	2 cut(s)	603, 2220
BbvI	GCAGC	7 cut(s)	997, 1037, 1190, 1363, 1990, 2243, 2661
BccI	CCATC	7 cut(s)	217, 1214, 1468, 1532, 2126, 2401, 2708
BciT130I	CCWGG	4 cut(s)	701, 2018, 2641, 2709
BciVI	GTATCC	1 cut(s)	1684
BclI	TGATCA	1 cut(s)	1957
BcoDI	GTCTC	4 cut(s)	41, 761, 1355, 2291
BfaI	CTAG	7 cut(s)	275, 1158, 1601, 1614, 1634, 2328, 2471
BfmI	CTRYAG	2 cut(s)	1689, 1779
BfoI	RGCGCY	1 cut(s)	1473
BfrI	CTTAAG	2 cut(s)	397, 1147
BfuAI	ACCTGC	2 cut(s)	1015, 1126
BfuI	GTATCC	1 cut(s)	1684
BglII	AGATCT	2 cut(s)	1901, 2311
BisI	GCNGC	8 cut(s)	1011, 1026, 1179, 1377, 1713, 2004, 2232, 2675
BlpI	GCTNAGC	2 cut(s)	186, 1878
BlsI	GCNGC	8 cut(s)	1012, 1027, 1180, 1378, 1714, 2005, 2233, 2676
BmcAI	AGTACT	1 cut(s)	817
Bme1390I	CCNGG	4 cut(s)	701, 2018, 2641, 2709
Bme18I	GGWCC	4 cut(s)	262, 544, 967, 2191
BmgBI	CACGTC	3 cut(s)	1643, 2227, 2665
BmgT120I	GGNCC	6 cut(s)	262, 544, 967, 1106, 1510, 2191
BmiI	GGNNCC	7 cut(s)	271, 292, 1005, 1107, 1116, 1452, 2097
BmrFI	CCNGG	4 cut(s)	701, 2018, 2641, 2709
BmtI	GCTAGC	1 cut(s)	1604
BpiI	GAAGAC	2 cut(s)	34, 2499
BpmI	CTGGAG	2 cut(s)	2502, 2730
Bpu10I	CCTNAGC	3 cut(s)	603, 2220, 2541
Bpu1102I	GCTNAGC	2 cut(s)	186, 1878
BpuEI	CTTGAG	5 cut(s)	1304, 1357, 1466, 2330, 2471
BsaAI	YACGTR	1 cut(s)	596
BsaI	GGTCTC	1 cut(s)	41
BsaJI	CCNNGG	2 cut(s)	265, 2602
BsaWI	WCCGGW	1 cut(s)	1978
BsaXI	ACNNNNNCTCC	6 cut(s)	143, 173, 457, 487, 2178, 2208
Bsc4I	CCNNNNNNNGG	3 cut(s)	1420, 1466, 2714
Bse118I	RCCGGY	1 cut(s)	1978
Bse1I	ACTGG	6 cut(s)	997, 1380, 1414, 1674, 1677, 2242
Bse3DI	GCAATG	4 cut(s)	118, 210, 1624, 2212
BseBI	CCWGG	4 cut(s)	701, 2018, 2641, 2709
BseDI	CCNNGG	2 cut(s)	265, 2602
BseGI	GGATG	6 cut(s)	1682, 1978, 2071, 2378, 2440, 2533
BseLI	CCNNNNNNNGG	3 cut(s)	1420, 1466, 2714
BseMI	GCAATG	4 cut(s)	118, 210, 1624, 2212
BseNI	ACTGG	6 cut(s)	997, 1380, 1414, 1674, 1677, 2242
BseRI	GAGGAG	3 cut(s)	428, 1978, 2186
BseSI	GKGCMC	2 cut(s)	274, 1006
BseXI	GCAGC	7 cut(s)	997, 1037, 1190, 1363, 1990, 2243, 2661
BseYI	CCCAGC	1 cut(s)	982
BsgI	GTGCAG	1 cut(s)	1044
Bsh1236I	CGCG	2 cut(s)	246, 1494
BshFI	GGCC	5 cut(s)	569, 1108, 1224, 1511, 2459
BshNI	GGYRCC	5 cut(s)	269, 290, 1003, 1450, 2095
BshTI	ACCGGT	1 cut(s)	1978
BsiHKAI	GWGCWC	3 cut(s)	179, 187, 2542
BsiSI	CCGG	1 cut(s)	1979
BslFI	GGGAC	2 cut(s)	305, 1629
BslI	CCNNNNNNNGG	3 cut(s)	1420, 1466, 2714
BsmAI	GTCTC	4 cut(s)	41, 761, 1355, 2291
BsmFI	GGGAC	2 cut(s)	305, 1629
BsmI	GAATGC	1 cut(s)	745
BsnI	GGCC	5 cut(s)	569, 1108, 1224, 1511, 2459
Bso31I	GGTCTC	1 cut(s)	41
Bsp1286I	GDGCHC	6 cut(s)	179, 187, 274, 1006, 1884, 2542
Bsp143I	GATC	4 cut(s)	1901, 1957, 2311, 2587
Bsp1720I	GCTNAGC	2 cut(s)	186, 1878
BspACI	CCGC	5 cut(s)	1686, 1712, 1885, 2149, 2332
BspANI	GGCC	5 cut(s)	569, 1108, 1224, 1511, 2459
BspFNI	CGCG	2 cut(s)	246, 1494
BspHI	TCATGA	1 cut(s)	1398
BspLI	GGNNCC	7 cut(s)	271, 292, 1005, 1107, 1116, 1452, 2097
BspMI	ACCTGC	2 cut(s)	1015, 1126
BspOI	GCTAGC	1 cut(s)	1604
BspQI	GCTCTTC	2 cut(s)	234, 465
BspT107I	GGYRCC	5 cut(s)	269, 290, 1003, 1450, 2095
BspTI	CTTAAG	2 cut(s)	397, 1147
BspTNI	GGTCTC	1 cut(s)	41
BsrDI	GCAATG	4 cut(s)	118, 210, 1624, 2212
BsrFI	RCCGGY	1 cut(s)	1978
BsrI	ACTGG	6 cut(s)	997, 1380, 1414, 1674, 1677, 2242
BssAI	RCCGGY	1 cut(s)	1978
BssECI	CCNNGG	2 cut(s)	265, 2602
BssMI	GATC	4 cut(s)	1901, 1957, 2311, 2587
BssSI	CACGAG	1 cut(s)	510
BssT1I	CCWWGG	1 cut(s)	2602
Bst2BI	CACGAG	1 cut(s)	510
Bst2UI	CCWGG	4 cut(s)	701, 2018, 2641, 2709
Bst6I	CTCTTC	6 cut(s)	234, 447, 465, 1525, 2417, 2715
BstAFI	CTTAAG	2 cut(s)	397, 1147
BstAPI	GCANNNNNTGC	2 cut(s)	1010, 1874
BstBAI	YACGTR	1 cut(s)	596
BstC8I	GCNNGC	9 cut(s)	123, 192, 1163, 1316, 1602, 2100, 2212, 2401, 2457
BstF5I	GGATG	6 cut(s)	1682, 1978, 2071, 2378, 2440, 2533
BstFNI	CGCG	2 cut(s)	246, 1494
BstH2I	RGCGCY	1 cut(s)	1473
BstHHI	GCGC	2 cut(s)	246, 1472
BstKTI	GATC	4 cut(s)	1904, 1960, 2314, 2590
BstMAI	GTCTC	4 cut(s)	41, 761, 1355, 2291
BstMBI	GATC	4 cut(s)	1901, 1957, 2311, 2587
BstMWI	GCNNNNNNNGC	9 cut(s)	191, 200, 1010, 1486, 1874, 2211, 2216, 2357, 2537
BstNI	CCWGG	4 cut(s)	701, 2018, 2641, 2709
BstNSI	RCATGY	3 cut(s)	314, 1303, 1318
BstSCI	CCNGG	4 cut(s)	699, 2016, 2639, 2707
BstSFI	CTRYAG	2 cut(s)	1689, 1779
BstSLI	GKGCMC	2 cut(s)	274, 1006
BstUI	CGCG	2 cut(s)	246, 1494
BstV1I	GCAGC	7 cut(s)	997, 1037, 1190, 1363, 1990, 2243, 2661
BstV2I	GAAGAC	2 cut(s)	34, 2499
BstX2I	RGATCY	2 cut(s)	1901, 2311
BstXI	CCANNNNNNTGG	2 cut(s)	1805, 2647
BstYI	RGATCY	2 cut(s)	1901, 2311
BsuI	GTATCC	1 cut(s)	1684
BsuRI	GGCC	5 cut(s)	569, 1108, 1224, 1511, 2459
BtgZI	GCGATG	1 cut(s)	1475
BtrI	CACGTC	3 cut(s)	1643, 2227, 2665
BtsCI	GGATG	6 cut(s)	1682, 1978, 2071, 2378, 2440, 2533
BtsI	GCAGTG	1 cut(s)	2097
BtsIMutI	CAGTG	6 cut(s)	354, 1004, 1387, 1421, 1698, 2097
BveI	ACCTGC	2 cut(s)	1015, 1126
Cac8I	GCNNGC	9 cut(s)	123, 192, 1163, 1316, 1602, 2100, 2212, 2401, 2457
CaiI	CAGNNNCTG	2 cut(s)	902, 2237
CciI	TCATGA	1 cut(s)	1398
CfoI	GCGC	2 cut(s)	246, 1472
Cfr10I	RCCGGY	1 cut(s)	1978
Cfr13I	GGNCC	6 cut(s)	262, 544, 967, 1106, 1510, 2191
CseI	GACGC	1 cut(s)	1227
CsiI	ACCWGGT	1 cut(s)	2016
Csp6I	GTAC	3 cut(s)	816, 1819, 1976
CspAI	ACCGGT	1 cut(s)	1978
CviAII	CATG	8 cut(s)	311, 1274, 1300, 1315, 1399, 1525, 2291, 2383
CviQI	GTAC	3 cut(s)	816, 1819, 1976
DpnI	GATC	4 cut(s)	1903, 1959, 2313, 2589
DpnII	GATC	4 cut(s)	1901, 1957, 2311, 2587
EaeI	YGGCCR	1 cut(s)	1222
Eam1104I	CTCTTC	6 cut(s)	234, 447, 465, 1525, 2417, 2715
EarI	CTCTTC	6 cut(s)	234, 447, 465, 1525, 2417, 2715
Ecl136II	GAGCTC	2 cut(s)	177, 2540
Eco130I	CCWWGG	1 cut(s)	2602
Eco147I	AGGCCT	1 cut(s)	569
Eco24I	GRGCYC	3 cut(s)	179, 1884, 2542
Eco31I	GGTCTC	1 cut(s)	41
Eco32I	GATATC	1 cut(s)	2686
Eco47I	GGWCC	4 cut(s)	262, 544, 967, 2191
Eco53kI	GAGCTC	2 cut(s)	177, 2540
Eco57I	CTGAAG	4 cut(s)	140, 1847, 2441, 2493
Eco72I	CACGTG	1 cut(s)	596
EcoICRI	GAGCTC	2 cut(s)	177, 2540
EcoO109I	RGGNCCY	1 cut(s)	2191
EcoRII	CCWGG	4 cut(s)	699, 2016, 2639, 2707
EcoRV	GATATC	1 cut(s)	2686
EcoT14I	CCWWGG	1 cut(s)	2602
EcoT22I	ATGCAT	4 cut(s)	745, 1040, 1491, 1561
EcoT38I	GRGCYC	3 cut(s)	179, 1884, 2542
ErhI	CCWWGG	1 cut(s)	2602
FaeI	CATG	8 cut(s)	314, 1277, 1303, 1318, 1402, 1528, 2294, 2386
FaqI	GGGAC	2 cut(s)	305, 1629
FatI	CATG	8 cut(s)	310, 1273, 1299, 1314, 1398, 1524, 2290, 2382
FauI	CCCGC	1 cut(s)	1892
FbaI	TGATCA	1 cut(s)	1957
Fnu4HI	GCNGC	8 cut(s)	1011, 1026, 1179, 1377, 1713, 2004, 2232, 2675
FokI	GGATG	6 cut(s)	1669, 1985, 2058, 2365, 2447, 2540
FriOI	GRGCYC	3 cut(s)	179, 1884, 2542
Fsp4HI	GCNGC	8 cut(s)	1011, 1026, 1179, 1377, 1713, 2004, 2232, 2675
FspBI	CTAG	7 cut(s)	275, 1158, 1601, 1614, 1634, 2328, 2471
GlaI	GCGC	2 cut(s)	245, 1471
GluI	GCNGC	8 cut(s)	1011, 1026, 1179, 1377, 1713, 2004, 2232, 2675
GsaI	CCCAGC	1 cut(s)	986
GsuI	CTGGAG	2 cut(s)	2502, 2730
HaeII	RGCGCY	1 cut(s)	1473
HaeIII	GGCC	5 cut(s)	569, 1108, 1224, 1511, 2459
HapII	CCGG	1 cut(s)	1979
HgaI	GACGC	1 cut(s)	1227
HhaI	GCGC	2 cut(s)	246, 1472
Hin1II	CATG	8 cut(s)	314, 1277, 1303, 1318, 1402, 1528, 2294, 2386
Hin6I	GCGC	2 cut(s)	244, 1470
HinP1I	GCGC	2 cut(s)	244, 1470
HincII	GTYRAC	2 cut(s)	1242, 1939
HindII	GTYRAC	2 cut(s)	1242, 1939
HindIII	AAGCTT	2 cut(s)	2212, 2630
HpaII	CCGG	1 cut(s)	1979
HphI	GGTGA	4 cut(s)	22, 1603, 1766, 2729
Hpy166II	GTNNAC	6 cut(s)	1242, 1297, 1939, 2014, 2065, 2668
Hpy188III	TCNNGA	9 cut(s)	369, 909, 1073, 1399, 1579, 1928, 2318, 2450, 2519
Hpy8I	GTNNAC	6 cut(s)	1242, 1297, 1939, 2014, 2065, 2668
Hpy99I	CGWCG	1 cut(s)	251
HpyAV	CCTTC	6 cut(s)	626, 665, 980, 1396, 1795, 2557
HpyCH4IV	ACGT	4 cut(s)	595, 1642, 2226, 2664
HpyF10VI	GCNNNNNNNGC	9 cut(s)	191, 200, 1010, 1486, 1874, 2211, 2216, 2357, 2537
HpySE526I	ACGT	4 cut(s)	595, 1642, 2226, 2664
Hsp92II	CATG	8 cut(s)	314, 1277, 1303, 1318, 1402, 1528, 2294, 2386
HspAI	GCGC	2 cut(s)	244, 1470
Ksp22I	TGATCA	1 cut(s)	1957
Kzo9I	GATC	4 cut(s)	1901, 1957, 2311, 2587
LguI	GCTCTTC	2 cut(s)	234, 465
LmnI	GCTCC	2 cut(s)	151, 200
Lsp1109I	GCAGC	7 cut(s)	997, 1037, 1190, 1363, 1990, 2243, 2661
MabI	ACCWGGT	1 cut(s)	2016
MaeI	CTAG	7 cut(s)	275, 1158, 1601, 1614, 1634, 2328, 2471
MaeII	ACGT	4 cut(s)	595, 1642, 2226, 2664
MaeIII	GTNAC	4 cut(s)	17, 483, 1320, 1591
MalI	GATC	4 cut(s)	1903, 1959, 2313, 2589
MboI	GATC	4 cut(s)	1901, 1957, 2311, 2587
MfeI	CAATTG	1 cut(s)	771
MflI	RGATCY	2 cut(s)	1901, 2311
MhlI	GDGCHC	6 cut(s)	179, 187, 274, 1006, 1884, 2542
MlsI	TGGCCA	1 cut(s)	1224
MluNI	TGGCCA	1 cut(s)	1224
MlyI	GAGTC	4 cut(s)	292, 1264, 1803, 2434
MmeI	TCCRAC	7 cut(s)	129, 843, 1786, 2316, 2439, 2491, 2628
Mox20I	TGGCCA	1 cut(s)	1224
Mph1103I	ATGCAT	4 cut(s)	745, 1040, 1491, 1561
MroXI	GAANNNNTTC	2 cut(s)	495, 2251
MscI	TGGCCA	1 cut(s)	1224
MslI	CAYNNNNRTG	1 cut(s)	1033
Msp20I	TGGCCA	1 cut(s)	1224
MspA1I	CMGCKG	2 cut(s)	190, 1181
MspCI	CTTAAG	2 cut(s)	397, 1147
MspI	CCGG	1 cut(s)	1979
MspR9I	CCNGG	4 cut(s)	701, 2018, 2641, 2709
MunI	CAATTG	1 cut(s)	771
Mva1269I	GAATGC	1 cut(s)	745
MvaI	CCWGG	4 cut(s)	701, 2018, 2641, 2709
MvnI	CGCG	2 cut(s)	246, 1494
MwoI	GCNNNNNNNGC	9 cut(s)	191, 200, 1010, 1486, 1874, 2211, 2216, 2357, 2537
NdeII	GATC	4 cut(s)	1901, 1957, 2311, 2587
NheI	GCTAGC	1 cut(s)	1600
NlaIII	CATG	8 cut(s)	314, 1277, 1303, 1318, 1402, 1528, 2294, 2386
NlaIV	GGNNCC	7 cut(s)	271, 292, 1005, 1107, 1116, 1452, 2097
NmuCI	GTSAC	1 cut(s)	1591
NsiI	ATGCAT	4 cut(s)	745, 1040, 1491, 1561
NspI	RCATGY	3 cut(s)	314, 1303, 1318
PaeI	GCATGC	1 cut(s)	1318
PagI	TCATGA	1 cut(s)	1398
PaqCI	CACCTGC	1 cut(s)	1015
PceI	AGGCCT	1 cut(s)	569
PciI	ACATGT	1 cut(s)	1299
PciSI	GCTCTTC	2 cut(s)	234, 465
PctI	GAATGC	1 cut(s)	745
PdmI	GAANNNNTTC	2 cut(s)	495, 2251
PfeI	GAWTC	9 cut(s)	334, 905, 1069, 1140, 1249, 1637, 1787, 2369, 2411
PfoI	TCCNGGA	1 cut(s)	699
PinAI	ACCGGT	1 cut(s)	1978
PkrI	GCNGC	8 cut(s)	1012, 1027, 1180, 1378, 1714, 2005, 2233, 2676
PleI	GAGTC	4 cut(s)	292, 1264, 1803, 2433
PmaCI	CACGTG	1 cut(s)	596
PmlI	CACGTG	1 cut(s)	596
PpsI	GAGTC	4 cut(s)	292, 1264, 1803, 2433
Ppu21I	YACGTR	1 cut(s)	596
PpuMI	RGGWCCY	1 cut(s)	2191
PscI	ACATGT	1 cut(s)	1299
PshBI	ATTAAT	3 cut(s)	788, 846, 873
Psp124BI	GAGCTC	2 cut(s)	179, 2542
Psp5II	RGGWCCY	1 cut(s)	2191
Psp6I	CCWGG	4 cut(s)	699, 2016, 2639, 2707
PspCI	CACGTG	1 cut(s)	596
PspFI	CCCAGC	1 cut(s)	982
PspGI	CCWGG	4 cut(s)	699, 2016, 2639, 2707
PspN4I	GGNNCC	7 cut(s)	271, 292, 1005, 1107, 1116, 1452, 2097
PspPI	GGNCC	6 cut(s)	262, 544, 967, 1106, 1510, 2191
PspPPI	RGGWCCY	1 cut(s)	2191
PstNI	CAGNNNCTG	2 cut(s)	902, 2237
PsuI	RGATCY	2 cut(s)	1901, 2311
PvuII	CAGCTG	2 cut(s)	190, 1181
RsaI	GTAC	3 cut(s)	817, 1820, 1977
RsaNI	GTAC	3 cut(s)	816, 1819, 1976
RseI	CAYNNNNRTG	1 cut(s)	1033
SacI	GAGCTC	2 cut(s)	179, 2542
SapI	GCTCTTC	2 cut(s)	234, 465
SatI	GCNGC	8 cut(s)	1011, 1026, 1179, 1377, 1713, 2004, 2232, 2675
Sau3AI	GATC	4 cut(s)	1901, 1957, 2311, 2587
Sau96I	GGNCC	6 cut(s)	262, 544, 967, 1106, 1510, 2191
ScaI	AGTACT	1 cut(s)	817
SchI	GAGTC	4 cut(s)	292, 1264, 1803, 2434
ScrFI	CCNGG	4 cut(s)	701, 2018, 2641, 2709
SduI	GDGCHC	6 cut(s)	179, 187, 274, 1006, 1884, 2542
SexAI	ACCWGGT	1 cut(s)	2016
SfcI	CTRYAG	2 cut(s)	1689, 1779
SinI	GGWCC	4 cut(s)	262, 544, 967, 2191
SmiMI	CAYNNNNRTG	1 cut(s)	1033
SmlI	CTYRAG	7 cut(s)	397, 1147, 1283, 1336, 1445, 2345, 2450
SmoI	CTYRAG	7 cut(s)	397, 1147, 1283, 1336, 1445, 2345, 2450
SphI	GCATGC	1 cut(s)	1318
SseBI	AGGCCT	1 cut(s)	569
SsiI	CCGC	5 cut(s)	1686, 1712, 1885, 2149, 2332
SspMI	CTAG	7 cut(s)	275, 1158, 1601, 1614, 1634, 2328, 2471
SstI	GAGCTC	2 cut(s)	179, 2542
StuI	AGGCCT	1 cut(s)	569
StyD4I	CCNGG	4 cut(s)	699, 2016, 2639, 2707
StyI	CCWWGG	1 cut(s)	2602
TaiI	ACGT	4 cut(s)	598, 1645, 2229, 2667
TaqI	TCGA	2 cut(s)	711, 2625
TatI	WGTACW	1 cut(s)	815
TauI	GCSGC	1 cut(s)	1715
TfiI	GAWTC	9 cut(s)	334, 905, 1069, 1140, 1249, 1637, 1787, 2369, 2411
TscAI	CASTG	6 cut(s)	361, 1004, 1387, 1421, 1698, 2097
TseFI	GTSAC	1 cut(s)	1591
TseI	GCWGC	7 cut(s)	1010, 1025, 1178, 1376, 2003, 2231, 2674
Tsp45I	GTSAC	1 cut(s)	1591
TspDTI	ATGAA	8 cut(s)	87, 365, 1415, 1541, 1613, 1671, 1851, 2279
TspGWI	ACGGA	1 cut(s)	1007
TspRI	CASTG	6 cut(s)	361, 1004, 1387, 1421, 1698, 2097
Vha464I	CTTAAG	2 cut(s)	397, 1147
VpaK11BI	GGWCC	4 cut(s)	262, 544, 967, 2191
VspI	ATTAAT	3 cut(s)	788, 846, 873
XapI	RAATTY	5 cut(s)	469, 609, 1169, 2159, 2392
XceI	RCATGY	3 cut(s)	314, 1303, 1318
XmnI	GAANNNNTTC	2 cut(s)	495, 2251
XspI	CTAG	7 cut(s)	275, 1158, 1601, 1614, 1634, 2328, 2471
ZrmI	AGTACT	1 cut(s)	817
Zsp2I	ATGCAT	4 cut(s)	745, 1040, 1491, 1561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.